MD17G1066000.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001665
Physical Location & Seq
Forward (+)
5334595 .. 5336282
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1066000.v1.1.491

Sequence Viewer

Length: 960 bp
ATGTTTCCAAGGGATTTCTCACATACCAGCCATTTCATTTCAGGGGCTTCTGTTGGTGTGGAATGCAAAGATGGGAGTTCAACAGAACAAAGTTTCAAGGCACAAGTGAAAACAAATAGCCATGGACAATTCAAAGTCCTTCTGCCATTCTCGGTAAGCAAACATGTCAAGAAAATTGAAGGATGCTCAGTGAAACTAATCAGCAGTAGTCAACCCTACTGTGCTGTGGCCTCATCAGCCACCTCCTCTTCCTTACACCTCAAGGCAAGAAAGCAAGGGACTCACATATTCTCTGCTGGTTTCTTCACCTTCAAGCCTCTCAAACAACCCACCTTATGCAACCAGAAACCCAGCATTCAGAATTCCAAGAAATTAAATTCGCAGAAGTTCTCATTCCCTCATACTGATGAGCTGGAATTCCCATCACCCTCTCAAGTGGACCCCACAATTCCTGATCTACCTCCCGTCCCTAAGATCCCTTATCTTCCACCGCTGCCACAACTCCCTCCTCTCCCTCCCATCCCAATTGGTGTCCCAGGACTCCCAGGACTTCCTGGAATTCCAGGTTCGCCCCCTGTTGCTGTAAAAACTTCATCTGTGTCCTCCAACTCCAAAACTTCAAAGAATGAGCAACTAGGTGATAATAAAGAAGTAGCGCATCCAGATACTATATTTCCACCTTTATTTCCACCAATTATCCCTAATCCATTTCAGCCCCCACCATCTCCATTAATCCCTAATTTGCCCCCTCTTCCTGGTTCTCCACCAGCAGGTCCAGCACCATTGATTCCCAACCTGCCAATTCCCAACCTGCCACCTATATCTGGTTTGACTCCATCGCCGTCACCACCACCACCAATCCTCCCATTCCTCCCTCCACTCCCCTTCCCACTGCCACCTGTGCCTCGGATCCCCGGAATTCCCCCAGCAGCTTCTTCAAAACAAAGTACTTCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

320

Amino Acids

33.67

Weight (kDa)

9.6

Isoelectric Point (pI)

66.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 7 - 81 2e-15 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 761, 804, 819
AciI CCGC 1 cut(s) 491
AclWI GGATC 3 cut(s) 469, 904, 917
AcsI RAATTY 5 cut(s) 361, 376, 416, 558, 918
AfaI GTAC 1 cut(s) 949
AfiI CCNNNNNNNGG 3 cut(s) 755, 770, 824
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 7 cut(s) 81, 97, 133, 179, 313, 621, 939
AjnI CCWGG 5 cut(s) 535, 544, 553, 562, 754
AluBI AGCT 2 cut(s) 412, 932
AluI AGCT 2 cut(s) 412, 932
AlwI GGATC 3 cut(s) 469, 904, 917
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 493, 929
ApoI RAATTY 5 cut(s) 361, 376, 416, 558, 918
AseI ATTAAT 1 cut(s) 731
AspLEI GCGC 1 cut(s) 658
AspS9I GGNCC 2 cut(s) 439, 773
AsuC2I CCSGG 1 cut(s) 915
AsuHPI GGTGA 5 cut(s) 298, 417, 650, 837, 945
AvaII GGWCC 2 cut(s) 439, 773
BamHI GGATCC 1 cut(s) 909
BbvI GCAGC 2 cut(s) 480, 941
BccI CCATC 5 cut(s) 65, 430, 527, 730, 844
BceAI ACGGC 1 cut(s) 826
BciT130I CCWGG 5 cut(s) 537, 546, 555, 564, 756
BcnI CCSGG 1 cut(s) 915
BfaI CTAG 1 cut(s) 635
BfuAI ACCTGC 3 cut(s) 761, 804, 819
BisI GCNGC 2 cut(s) 494, 930
BlsI GCNGC 2 cut(s) 495, 931
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 6 cut(s) 537, 546, 555, 564, 756, 915
Bme18I GGWCC 2 cut(s) 439, 773
BmgT120I GGNCC 2 cut(s) 439, 773
BmiI GGNNCC 2 cut(s) 441, 911
BmrFI CCNGG 6 cut(s) 537, 546, 555, 564, 756, 915
BmsI GCATC 2 cut(s) 173, 667
BpuEI CTTGAG 2 cut(s) 245, 417
BpuMI CCSGG 1 cut(s) 915
BsaJI CCNNGG 6 cut(s) 8, 121, 535, 544, 905, 913
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 3 cut(s) 755, 770, 824
BseBI CCWGG 5 cut(s) 537, 546, 555, 564, 756
BseDI CCNNGG 6 cut(s) 8, 121, 535, 544, 905, 913
BseGI GGATG 3 cut(s) 188, 519, 658
BseLI CCNNNNNNNGG 3 cut(s) 755, 770, 824
BseMII CTCAG 1 cut(s) 201
BseRI GAGGAG 2 cut(s) 235, 498
BseXI GCAGC 2 cut(s) 480, 941
BseYI CCCAGC 2 cut(s) 350, 925
BshFI GGCC 1 cut(s) 230
BsiSI CCGG 1 cut(s) 915
BslFI GGGAC 3 cut(s) 292, 452, 518
BslI CCNNNNNNNGG 3 cut(s) 755, 770, 824
BsmFI GGGAC 3 cut(s) 292, 452, 518
BsmI GAATGC 2 cut(s) 68, 354
BsnI GGCC 1 cut(s) 230
Bsp143I GATC 3 cut(s) 454, 474, 909
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 1 cut(s) 491
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 200
BspLI GGNNCC 2 cut(s) 441, 911
BspMI ACCTGC 3 cut(s) 761, 804, 819
BspPI GGATC 3 cut(s) 469, 904, 917
BssECI CCNNGG 6 cut(s) 8, 121, 535, 544, 905, 913
BssMI GATC 3 cut(s) 454, 474, 909
BssT1I CCWWGG 2 cut(s) 8, 121
Bst2UI CCWGG 5 cut(s) 537, 546, 555, 564, 756
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 2 cut(s) 253, 756
BstDEI CTNAG 2 cut(s) 187, 471
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 3 cut(s) 188, 519, 658
BstHHI GCGC 1 cut(s) 658
BstKTI GATC 3 cut(s) 457, 477, 912
BstMBI GATC 3 cut(s) 454, 474, 909
BstMWI GCNNNNNNNGC 3 cut(s) 236, 776, 901
BstNI CCWGG 5 cut(s) 537, 546, 555, 564, 756
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 6 cut(s) 535, 544, 553, 562, 754, 913
BstV1I GCAGC 2 cut(s) 480, 941
BstX2I RGATCY 2 cut(s) 474, 909
BstYI RGATCY 2 cut(s) 474, 909
BsuRI GGCC 1 cut(s) 230
BtgI CCRYGG 1 cut(s) 121
BtgZI GCGATG 1 cut(s) 822
BtsCI GGATG 3 cut(s) 188, 519, 658
BtsI GCAGTG 1 cut(s) 890
BtsIMutI CAGTG 2 cut(s) 195, 890
BveI ACCTGC 3 cut(s) 761, 804, 819
CfoI GCGC 1 cut(s) 658
Cfr13I GGNCC 2 cut(s) 439, 773
Csp6I GTAC 1 cut(s) 948
CviAII CATG 2 cut(s) 122, 164
CviJI RGCY 9 cut(s) 30, 47, 120, 230, 239, 316, 412, 715, 932
CviKI_1 RGCY 9 cut(s) 30, 47, 120, 230, 239, 316, 412, 715, 932
CviQI GTAC 1 cut(s) 948
DdeI CTNAG 2 cut(s) 187, 471
DpnI GATC 3 cut(s) 456, 476, 911
DpnII GATC 3 cut(s) 454, 474, 909
Eam1104I CTCTTC 2 cut(s) 253, 756
EarI CTCTTC 2 cut(s) 253, 756
Eco130I CCWWGG 2 cut(s) 8, 121
Eco47I GGWCC 2 cut(s) 439, 773
EcoRI GAATTC 4 cut(s) 361, 416, 558, 918
EcoRII CCWGG 5 cut(s) 535, 544, 553, 562, 754
EcoT14I CCWWGG 2 cut(s) 8, 121
ErhI CCWWGG 2 cut(s) 8, 121
FaeI CATG 2 cut(s) 125, 167
FaiI YATR 8 cut(s) 24, 123, 165, 287, 337, 402, 671, 821
FaqI GGGAC 3 cut(s) 292, 452, 518
FatI CATG 2 cut(s) 121, 163
Fnu4HI GCNGC 2 cut(s) 494, 930
FokI GGATG 3 cut(s) 195, 506, 645
Fsp4HI GCNGC 2 cut(s) 494, 930
FspBI CTAG 1 cut(s) 635
GlaI GCGC 1 cut(s) 657
GluI GCNGC 2 cut(s) 494, 930
GsaI CCCAGC 2 cut(s) 354, 929
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 915
HhaI GCGC 1 cut(s) 658
Hin1II CATG 2 cut(s) 125, 167
Hin6I GCGC 1 cut(s) 656
HinP1I GCGC 1 cut(s) 656
HincII GTYRAC 1 cut(s) 212
HindII GTYRAC 1 cut(s) 212
HinfI GANTC 4 cut(s) 280, 540, 787, 832
HpaII CCGG 1 cut(s) 915
HphI GGTGA 5 cut(s) 298, 417, 650, 837, 945
Hpy166II GTNNAC 2 cut(s) 212, 439
Hpy188I TCNGA 2 cut(s) 360, 909
Hpy188III TCNNGA 3 cut(s) 169, 452, 662
Hpy8I GTNNAC 2 cut(s) 212, 439
HpyAV CCTTC 4 cut(s) 149, 173, 319, 895
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 2 cut(s) 66, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 776, 901
HpyF3I CTNAG 2 cut(s) 187, 471
Hsp92II CATG 2 cut(s) 125, 167
HspAI GCGC 1 cut(s) 656
Kzo9I GATC 3 cut(s) 454, 474, 909
Lsp1109I GCAGC 2 cut(s) 480, 941
LweI GCATC 2 cut(s) 173, 667
MaeI CTAG 1 cut(s) 635
MaeIII GTNAC 1 cut(s) 843
MalI GATC 3 cut(s) 456, 476, 911
MboI GATC 3 cut(s) 454, 474, 909
MboII GAAGA 5 cut(s) 240, 295, 476, 743, 927
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 2 cut(s) 474, 909
MlyI GAGTC 3 cut(s) 274, 534, 826
MmeI TCCRAC 1 cut(s) 630
MseI TTAA 2 cut(s) 374, 731
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 1 cut(s) 915
MspR9I CCNGG 6 cut(s) 537, 546, 555, 564, 756, 915
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 2 cut(s) 68, 354
MvaI CCWGG 5 cut(s) 537, 546, 555, 564, 756
MwoI GCNNNNNNNGC 3 cut(s) 236, 776, 901
NciI CCSGG 1 cut(s) 915
NcoI CCATGG 1 cut(s) 121
NdeII GATC 3 cut(s) 454, 474, 909
NlaIII CATG 2 cut(s) 125, 167
NlaIV GGNNCC 2 cut(s) 441, 911
NmuCI GTSAC 1 cut(s) 843
NspI RCATGY 1 cut(s) 167
PciI ACATGT 1 cut(s) 163
PctI GAATGC 2 cut(s) 68, 354
PfeI GAWTC 1 cut(s) 787
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 2 cut(s) 495, 931
PleI GAGTC 3 cut(s) 274, 534, 826
PpsI GAGTC 3 cut(s) 274, 534, 826
PscI ACATGT 1 cut(s) 163
PshBI ATTAAT 1 cut(s) 731
Psp6I CCWGG 5 cut(s) 535, 544, 553, 562, 754
PspFI CCCAGC 2 cut(s) 350, 925
PspGI CCWGG 5 cut(s) 535, 544, 553, 562, 754
PspN4I GGNNCC 2 cut(s) 441, 911
PspPI GGNCC 2 cut(s) 439, 773
PsuI RGATCY 2 cut(s) 474, 909
RsaI GTAC 1 cut(s) 949
RsaNI GTAC 1 cut(s) 948
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 2 cut(s) 374, 731
SatI GCNGC 2 cut(s) 494, 930
Sau3AI GATC 3 cut(s) 454, 474, 909
Sau96I GGNCC 2 cut(s) 439, 773
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 3 cut(s) 274, 534, 826
ScrFI CCNGG 6 cut(s) 537, 546, 555, 564, 756, 915
SfaNI GCATC 2 cut(s) 173, 667
SinI GGWCC 2 cut(s) 439, 773
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 2 cut(s) 260, 432
SmoI CTYRAG 2 cut(s) 260, 432
SsiI CCGC 1 cut(s) 491
SspMI CTAG 1 cut(s) 635
StyD4I CCNGG 6 cut(s) 535, 544, 553, 562, 754, 913
StyI CCWWGG 2 cut(s) 8, 121
TaaI ACNGT 1 cut(s) 221
TatI WGTACW 1 cut(s) 947
TfiI GAWTC 1 cut(s) 787
Tru1I TTAA 2 cut(s) 374, 731
Tru9I TTAA 2 cut(s) 374, 731
TscAI CASTG 2 cut(s) 195, 897
TseFI GTSAC 1 cut(s) 843
TseI GCWGC 2 cut(s) 493, 929
Tsp45I GTSAC 1 cut(s) 843
TspDTI ATGAA 2 cut(s) 25, 582
TspRI CASTG 2 cut(s) 195, 897
VpaK11BI GGWCC 2 cut(s) 439, 773
VspI ATTAAT 1 cut(s) 731
XapI RAATTY 5 cut(s) 361, 376, 416, 558, 918
XceI RCATGY 1 cut(s) 167
XspI CTAG 1 cut(s) 635
ZrmI AGTACT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.