Rroxscaffold_2G00086280

Pollen Ole e 1 allergen and extensin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001665
Physical Location & Seq
Forward (+)
8509663 .. 8511018
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086280.1

Sequence Viewer

Length: 1017 bp
ATGTCTTGGTTTCTAACAATATTCTTCCTCATTAGTTTCACATTTAGTACTCCTTCAGAGGCTAACCATGAGAAGAAGATTCAACCTGCTGTGGTTATAGGTAAAGTTTACTGTGAGACATGTTTCCAAGATGGGTTCTCAAAATCCAGCCACTTCATTTCAGGGGCTACTGTTGGTGTGGAGTGCAAAGATCAGACTTCATCAAAACCCAGCTTTCAAGCCCAAGTGAAGACAGATAACCAAGGAGTGTTCAAAGTCCAATTGCCTTTCTCAGTGAGCAAACATGTGAAGAAAATTGAAGGGTGTTCTGTGAAGCTGATTCGCAGCAGCGAACCGTATTGCGCTGTGGCCTCAGCAGCAGCTTCATCTTCACTTCACCTCAAATCAAGGAAGCAAGGAACTCACATTTTCTCAGCTGGGTTTTTCACCTTCAAGCCCCTAAACCAACCCAACCTATGTAACCAAAAACCAAGCCTCATCCAAAACAACAAGAAGGAAGTTATCACTAATTCCAAGAAACTCTCATTTTCTCCAACTGAGGATTTGACATTTCCCTCACCAGTTCAAGAACCCAGCTTTCCTGGTGTTCCTTCTCTCCCTGTACTTCCTTATCTTCCACCACTACCACAACTCCCTCCTCTCCCAGGACTCCCAGGTCTTCCAGGAATGGGAATTCCAACTTTACCCCCATCCCAGGAAAAGCACCTAACTGAAGAAGAAAAACTAGCCCGCCCAGATTTTTTCTTCCCACCGAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTTCTGCCAAGCCCACCTCCACTTATCCCAAACCCATTCCAGCCACCACCAGCACCATTGATTCCACATTTGCCACCATTGCCTAGCTTGCCACCACTACCAAGTTTAACTCCACCATCTCCACCACCACCATCATTCCCAACAATCCCTCCATTGCTTCCGTTCCCACTCCCACCTGTACCTCTTATTCCTGGAATCCCCCTGCTTCTTCCTCTCCTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

338

Amino Acids

36.58

Weight (kDa)

8.97

Isoelectric Point (pI)

68.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 32 - 120 3e-20 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 654
Acc36I ACCTGC 1 cut(s) 94
AciI CCGC 1 cut(s) 730
AcsI RAATTY 1 cut(s) 672
AcuI CTGAAG 2 cut(s) 39, 732
AfaI GTAC 3 cut(s) 49, 603, 972
AfiI CCNNNNNNNGG 3 cut(s) 644, 668, 694
AflIII ACRYGT 2 cut(s) 119, 283
AgsI TTSAA 6 cut(s) 83, 218, 253, 299, 433, 566
AjnI CCWGG 6 cut(s) 580, 643, 652, 661, 693, 982
AluBI AGCT 6 cut(s) 213, 316, 362, 416, 576, 879
AluI AGCT 6 cut(s) 213, 316, 362, 416, 576, 879
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 4 cut(s) 324, 327, 356, 359
ApoI RAATTY 1 cut(s) 672
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 3 cut(s) 368, 418, 549
BbsI GAAGAC 2 cut(s) 236, 650
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 4 cut(s) 336, 339, 368, 371
BccI CCATC 5 cut(s) 125, 697, 778, 916, 931
BciT130I CCWGG 6 cut(s) 582, 645, 654, 663, 695, 984
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 725, 876
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 4 cut(s) 325, 328, 357, 360
BlsI GCNGC 4 cut(s) 326, 329, 358, 361
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 6 cut(s) 582, 645, 654, 663, 695, 984
BmrFI CCNGG 6 cut(s) 582, 645, 654, 663, 695, 984
BpiI GAAGAC 2 cut(s) 236, 650
Bpu10I CCTNAGC 1 cut(s) 352
BsaJI CCNNGG 4 cut(s) 241, 643, 652, 693
BsaXI ACNNNNNCTCC 4 cut(s) 615, 645, 925, 955
Bsc4I CCNNNNNNNGG 3 cut(s) 644, 668, 694
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 2 cut(s) 869, 944
BseBI CCWGG 6 cut(s) 582, 645, 654, 663, 695, 984
BseDI CCNNGG 4 cut(s) 241, 643, 652, 693
BseGI GGATG 2 cut(s) 477, 689
BseLI CCNNNNNNNGG 3 cut(s) 644, 668, 694
BseMI GCAATG 2 cut(s) 869, 944
BseMII CTCAG 4 cut(s) 285, 366, 426, 528
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 4 cut(s) 336, 339, 368, 371
BseYI CCCAGC 3 cut(s) 209, 416, 572
BshFI GGCC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 644, 668, 694
BsmAI GTCTC 1 cut(s) 110
BsnI GGCC 1 cut(s) 350
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 730
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 4 cut(s) 284, 365, 425, 529
BspMI ACCTGC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 869, 944
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 4 cut(s) 241, 643, 652, 693
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 6 cut(s) 582, 645, 654, 663, 695, 984
Bst4CI ACNGT 3 cut(s) 113, 172, 336
Bst6I CTCTTC 1 cut(s) 798
BstC8I GCNNGC 2 cut(s) 730, 881
BstDEI CTNAG 4 cut(s) 271, 352, 412, 537
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 2 cut(s) 477, 689
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 356, 871, 880
BstNI CCWGG 6 cut(s) 582, 645, 654, 663, 695, 984
BstNSI RCATGY 2 cut(s) 123, 287
BstSCI CCNGG 6 cut(s) 580, 643, 652, 661, 693, 982
BstV1I GCAGC 4 cut(s) 336, 339, 368, 371
BstV2I GAAGAC 2 cut(s) 236, 650
BsuRI GGCC 1 cut(s) 350
BtsCI GGATG 2 cut(s) 477, 689
BtsIMutI CAGTG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 730, 881
CfoI GCGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 48, 602, 971
CviAII CATG 3 cut(s) 68, 120, 284
CviQI GTAC 3 cut(s) 48, 602, 971
DdeI CTNAG 4 cut(s) 271, 352, 412, 537
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DrdI GACNNNNNNGTC 1 cut(s) 654
DseDI GACNNNNNNGTC 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Eco130I CCWWGG 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 39, 732
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 6 cut(s) 580, 643, 652, 661, 693, 982
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 3 cut(s) 71, 123, 287
FaiI YATR 5 cut(s) 69, 98, 121, 285, 457
FatI CATG 3 cut(s) 67, 119, 283
FauI CCCGC 1 cut(s) 737
Fnu4HI GCNGC 4 cut(s) 325, 328, 357, 360
FokI GGATG 2 cut(s) 464, 676
Fsp4HI GCNGC 4 cut(s) 325, 328, 357, 360
FspBI CTAG 2 cut(s) 725, 876
GlaI GCGC 1 cut(s) 343
GluI GCNGC 4 cut(s) 325, 328, 357, 360
GsaI CCCAGC 3 cut(s) 213, 420, 576
HaeIII GGCC 1 cut(s) 350
HhaI GCGC 1 cut(s) 344
Hin1II CATG 3 cut(s) 71, 123, 287
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 5 cut(s) 79, 319, 648, 853, 987
HphI GGTGA 3 cut(s) 368, 418, 549
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 58, 195
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 5 cut(s) 63, 293, 439, 487, 600
HpyCH4III ACNGT 3 cut(s) 113, 172, 336
HpyCH4V TGCA 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 871, 880
HpyF3I CTNAG 4 cut(s) 271, 352, 412, 537
Hsp92II CATG 3 cut(s) 71, 123, 287
HspAI GCGC 1 cut(s) 342
Kzo9I GATC 1 cut(s) 190
Lsp1109I GCAGC 4 cut(s) 336, 339, 368, 371
MaeI CTAG 2 cut(s) 725, 876
MaeIII GTNAC 1 cut(s) 458
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MfeI CAATTG 1 cut(s) 260
MluCI AATT 5 cut(s) 260, 294, 508, 672, 774
MlyI GAGTC 1 cut(s) 642
MmeI TCCRAC 2 cut(s) 557, 701
MseI TTAA 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 6 cut(s) 582, 645, 654, 663, 695, 984
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 6 cut(s) 582, 645, 654, 663, 695, 984
MwoI GCNNNNNNNGC 3 cut(s) 356, 871, 880
NdeII GATC 1 cut(s) 190
NlaIII CATG 3 cut(s) 71, 123, 287
NspI RCATGY 2 cut(s) 123, 287
PciI ACATGT 2 cut(s) 119, 283
PfeI GAWTC 4 cut(s) 79, 319, 853, 987
PfoI TCCNGGA 2 cut(s) 661, 982
PkrI GCNGC 4 cut(s) 326, 329, 358, 361
PleI GAGTC 1 cut(s) 642
PpsI GAGTC 1 cut(s) 642
PscI ACATGT 2 cut(s) 119, 283
Psp6I CCWGG 6 cut(s) 580, 643, 652, 661, 693, 982
PspFI CCCAGC 3 cut(s) 209, 416, 572
PspGI CCWGG 6 cut(s) 580, 643, 652, 661, 693, 982
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 3 cut(s) 49, 603, 972
RsaNI GTAC 3 cut(s) 48, 602, 971
SaqAI TTAA 1 cut(s) 899
SatI GCNGC 4 cut(s) 325, 328, 357, 360
Sau3AI GATC 1 cut(s) 190
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 6 cut(s) 582, 645, 654, 663, 695, 984
Sse9I AATT 5 cut(s) 260, 294, 508, 672, 774
SsiI CCGC 1 cut(s) 730
SspI AATATT 1 cut(s) 21
SspMI CTAG 2 cut(s) 725, 876
StyD4I CCNGG 6 cut(s) 580, 643, 652, 661, 693, 982
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 3 cut(s) 113, 172, 336
TasI AATT 5 cut(s) 260, 294, 508, 672, 774
TatI WGTACW 2 cut(s) 47, 601
TfiI GAWTC 4 cut(s) 79, 319, 853, 987
Tru1I TTAA 1 cut(s) 899
Tru9I TTAA 1 cut(s) 899
TscAI CASTG 1 cut(s) 279
TseI GCWGC 4 cut(s) 324, 327, 356, 359
TspDTI ATGAA 3 cut(s) 145, 189, 354
TspGWI ACGGA 1 cut(s) 942
TspRI CASTG 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 1 cut(s) 672
XceI RCATGY 2 cut(s) 123, 287
XspI CTAG 2 cut(s) 725, 876
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.