pycom09g00240

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001665
Physical Location & Seq
Forward (+)
217505 .. 218774
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00240.2

Sequence Viewer

Length: 804 bp
ATGTCTTGGTTTCTTACGGTTATCTTTCTGGTTACTACTCTTACATTTCATACTCCTGCAGAGGCTAGTTATGAGGAGATCCAACCTTCTGCAGTAGTTGTTGGCACTGTCTACTGTGACACATGTTTCCAAGAGGATTTCTCAAATACCAGCCACTTCATTTCAGGGGCTTCTGTTGGTGTCGAATGCAAAGATGGGACTTCAACAAAACCAAGTTTCAACGCACAAGTGAAAACAAACAGCCATGGGCAATTCAAAGTCCATCTGCCCTTCTCAGTGAGCAAAGATGTCAAGAAAATTGAAGGATGCTCCGTGAAACTAATCCGCAGCAGTGAACCCTACTGTGCTGTGGCCTCATCAGCCACCTCATCTTCCCTACACCTCAAGGCAAGAAAGCAAGGGACTCACATATTCTCTGCTGGTTTCTTCACCTTCAAGCCTCTCAAGCAACCCAGCTTATGCAACCAGAAACCCAGCATTCAGAATTCCAAGAAATTAAATTCCCAGGAGTTCTCAGAGATGGCATTCCCATCCTCCTCTCAGGTGAACCCCAAAATGCCTGATCCCTCTCTCAACCAATCTGATCTCCTAGGTCTCCCAATCGATCTCCCAGGACTCCCAGGTCTCCCAGGACTCCCAGGACTCCCAGGTCTCCCAGGCCTCCCAGGACTTCCAGGGCTGCCCCTTGCTGCCGAAAAAACTACATCTAAGTCCTCCAACTCCAAAACTTCAAAGAATGGGCAACTAGGTGATAATAAGGAAGTAGCCCATCCAGATTCTCCACCAGCAGGTTCAGCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

268

Amino Acids

28.28

Weight (kDa)

8.47

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 621, 648
Acc36I ACCTGC 1 cut(s) 779
AccI GTMKAC 1 cut(s) 111
AciI CCGC 1 cut(s) 325
AclWI GGATC 2 cut(s) 73, 557
AcsI RAATTY 2 cut(s) 484, 499
AfiI CCNNNNNNNGG 1 cut(s) 788
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 6 cut(s) 204, 220, 256, 302, 436, 732
AjnI CCWGG 9 cut(s) 504, 610, 619, 628, 637, 646, 655, 664, 673
AluBI AGCT 1 cut(s) 456
AluI AGCT 1 cut(s) 456
Alw26I GTCTC 3 cut(s) 599, 629, 656
AlwI GGATC 2 cut(s) 73, 557
AoxI GGCC 2 cut(s) 351, 658
ApeKI GCWGC 3 cut(s) 327, 679, 689
ApoI RAATTY 2 cut(s) 484, 499
AspA2I CCTAGG 1 cut(s) 589
AsuHPI GGTGA 3 cut(s) 421, 556, 761
AvrII CCTAGG 1 cut(s) 589
BbvI GCAGC 3 cut(s) 339, 666, 676
BccI CCATC 5 cut(s) 188, 270, 514, 538, 777
BciT130I CCWGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
BcoDI GTCTC 3 cut(s) 599, 629, 656
BfaI CTAG 3 cut(s) 66, 590, 746
BfmI CTRYAG 2 cut(s) 57, 90
BfuAI ACCTGC 1 cut(s) 779
BisI GCNGC 3 cut(s) 328, 680, 690
BlnI CCTAGG 1 cut(s) 589
BlsI GCNGC 3 cut(s) 329, 681, 691
Bme1390I CCNGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
BmrFI CCNGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
BmsI GCATC 1 cut(s) 296
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpuEI CTTGAG 2 cut(s) 368, 428
Bsa29I ATCGAT 1 cut(s) 603
BsaI GGTCTC 3 cut(s) 599, 629, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 788
BseBI CCWGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
BseCI ATCGAT 1 cut(s) 603
BseGI GGATG 3 cut(s) 311, 530, 769
BseLI CCNNNNNNNGG 1 cut(s) 788
BseMII CTCAG 3 cut(s) 288, 528, 554
BseRI GAGGAG 2 cut(s) 89, 526
BseXI GCAGC 3 cut(s) 339, 666, 676
BseYI CCCAGC 2 cut(s) 452, 473
BshFI GGCC 2 cut(s) 353, 660
BshVI ATCGAT 1 cut(s) 603
BslFI GGGAC 2 cut(s) 211, 415
BslI CCNNNNNNNGG 1 cut(s) 788
BsmAI GTCTC 3 cut(s) 599, 629, 656
BsmFI GGGAC 2 cut(s) 211, 415
BsmI GAATGC 3 cut(s) 191, 477, 524
BsnI GGCC 2 cut(s) 353, 660
Bso31I GGTCTC 3 cut(s) 599, 629, 656
Bsp143I GATC 4 cut(s) 78, 562, 583, 604
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 325
BspANI GGCC 2 cut(s) 353, 660
BspCNI CTCAG 3 cut(s) 287, 527, 553
BspDI ATCGAT 1 cut(s) 603
BspMAI CTGCAG 2 cut(s) 61, 94
BspMI ACCTGC 1 cut(s) 779
BspPI GGATC 2 cut(s) 73, 557
BspTNI GGTCTC 3 cut(s) 599, 629, 656
BssMI GATC 4 cut(s) 78, 562, 583, 604
BssT1I CCWWGG 2 cut(s) 244, 589
Bst2UI CCWGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
Bst4CI ACNGT 4 cut(s) 19, 109, 116, 344
BstDEI CTNAG 4 cut(s) 274, 514, 540, 708
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 3 cut(s) 311, 530, 769
BstKTI GATC 4 cut(s) 81, 565, 586, 607
BstMAI GTCTC 3 cut(s) 599, 629, 656
BstMBI GATC 4 cut(s) 78, 562, 583, 604
BstMWI GCNNNNNNNGC 3 cut(s) 359, 445, 794
BstNI CCWGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 9 cut(s) 504, 610, 619, 628, 637, 646, 655, 664, 673
BstSFI CTRYAG 2 cut(s) 57, 90
BstV1I GCAGC 3 cut(s) 339, 666, 676
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 603
BsuRI GGCC 2 cut(s) 353, 660
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 311, 530, 769
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 3 cut(s) 105, 282, 337
BveI ACCTGC 1 cut(s) 779
ClaI ATCGAT 1 cut(s) 603
CviAII CATG 3 cut(s) 123, 245, 801
DdeI CTNAG 4 cut(s) 274, 514, 540, 708
DpnI GATC 4 cut(s) 80, 564, 585, 606
DpnII GATC 4 cut(s) 78, 562, 583, 604
DrdI GACNNNNNNGTC 2 cut(s) 621, 648
DseDI GACNNNNNNGTC 2 cut(s) 621, 648
Eco130I CCWWGG 2 cut(s) 244, 589
Eco147I AGGCCT 1 cut(s) 660
Eco31I GGTCTC 3 cut(s) 599, 629, 656
EcoRI GAATTC 1 cut(s) 484
EcoRII CCWGG 9 cut(s) 504, 610, 619, 628, 637, 646, 655, 664, 673
EcoT14I CCWWGG 2 cut(s) 244, 589
ErhI CCWWGG 2 cut(s) 244, 589
FaeI CATG 3 cut(s) 126, 248, 804
FaiI YATR 7 cut(s) 51, 72, 124, 246, 410, 460, 802
FaqI GGGAC 2 cut(s) 211, 415
FatI CATG 3 cut(s) 122, 244, 800
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 328, 680, 690
FokI GGATG 3 cut(s) 318, 517, 756
Fsp4HI GCNGC 3 cut(s) 328, 680, 690
FspBI CTAG 3 cut(s) 66, 590, 746
GluI GCNGC 3 cut(s) 328, 680, 690
GsaI CCCAGC 2 cut(s) 456, 477
HaeIII GGCC 2 cut(s) 353, 660
Hin1II CATG 3 cut(s) 126, 248, 804
HinfI GANTC 5 cut(s) 403, 615, 633, 642, 776
HphI GGTGA 3 cut(s) 421, 556, 761
Hpy166II GTNNAC 3 cut(s) 112, 335, 547
Hpy188I TCNGA 3 cut(s) 483, 517, 583
Hpy188III TCNNGA 2 cut(s) 292, 773
Hpy8I GTNNAC 3 cut(s) 112, 335, 547
HpyAV CCTTC 4 cut(s) 96, 280, 296, 442
HpyCH4III ACNGT 4 cut(s) 19, 109, 116, 344
HpyCH4V TGCA 4 cut(s) 59, 92, 189, 462
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 445, 794
HpyF3I CTNAG 4 cut(s) 274, 514, 540, 708
Hsp92II CATG 3 cut(s) 126, 248, 804
Kzo9I GATC 4 cut(s) 78, 562, 583, 604
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 3 cut(s) 339, 666, 676
LweI GCATC 1 cut(s) 296
MaeI CTAG 3 cut(s) 66, 590, 746
MaeIII GTNAC 2 cut(s) 31, 116
MalI GATC 4 cut(s) 80, 564, 585, 606
MboI GATC 4 cut(s) 78, 562, 583, 604
MboII GAAGA 2 cut(s) 363, 418
MflI RGATCY 1 cut(s) 78
MluCI AATT 5 cut(s) 251, 297, 484, 494, 499
MlyI GAGTC 4 cut(s) 397, 609, 627, 636
MmeI TCCRAC 2 cut(s) 106, 741
MseI TTAA 1 cut(s) 497
MspR9I CCNGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
Mva1269I GAATGC 3 cut(s) 191, 477, 524
MvaI CCWGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
MwoI GCNNNNNNNGC 3 cut(s) 359, 445, 794
NcoI CCATGG 1 cut(s) 244
NdeII GATC 4 cut(s) 78, 562, 583, 604
NlaIII CATG 3 cut(s) 126, 248, 804
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 126
PceI AGGCCT 1 cut(s) 660
PciI ACATGT 1 cut(s) 122
PctI GAATGC 3 cut(s) 191, 477, 524
PfeI GAWTC 1 cut(s) 776
PkrI GCNGC 3 cut(s) 329, 681, 691
PleI GAGTC 4 cut(s) 397, 609, 627, 636
PpsI GAGTC 4 cut(s) 397, 609, 627, 636
PscI ACATGT 1 cut(s) 122
Psp6I CCWGG 9 cut(s) 504, 610, 619, 628, 637, 646, 655, 664, 673
PspFI CCCAGC 2 cut(s) 452, 473
PspGI CCWGG 9 cut(s) 504, 610, 619, 628, 637, 646, 655, 664, 673
PstI CTGCAG 2 cut(s) 61, 94
PsuI RGATCY 1 cut(s) 78
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 3 cut(s) 328, 680, 690
Sau3AI GATC 4 cut(s) 78, 562, 583, 604
SchI GAGTC 4 cut(s) 397, 609, 627, 636
ScrFI CCNGG 9 cut(s) 506, 612, 621, 630, 639, 648, 657, 666, 675
SfaNI GCATC 1 cut(s) 296
SfcI CTRYAG 2 cut(s) 57, 90
SmlI CTYRAG 2 cut(s) 383, 443
SmoI CTYRAG 2 cut(s) 383, 443
Sse9I AATT 5 cut(s) 251, 297, 484, 494, 499
SseBI AGGCCT 1 cut(s) 660
SsiI CCGC 1 cut(s) 325
SspMI CTAG 3 cut(s) 66, 590, 746
StuI AGGCCT 1 cut(s) 660
StyD4I CCNGG 9 cut(s) 504, 610, 619, 628, 637, 646, 655, 664, 673
StyI CCWWGG 2 cut(s) 244, 589
TaaI ACNGT 4 cut(s) 19, 109, 116, 344
TaqI TCGA 2 cut(s) 183, 603
TasI AATT 5 cut(s) 251, 297, 484, 494, 499
TfiI GAWTC 1 cut(s) 776
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 3 cut(s) 112, 282, 337
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 3 cut(s) 327, 679, 689
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 2 cut(s) 38, 148
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 3 cut(s) 112, 282, 337
XapI RAATTY 2 cut(s) 484, 499
XceI RCATGY 1 cut(s) 126
XmaJI CCTAGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 3 cut(s) 66, 590, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.