Rh2BG590000

Pollen Ole e 1 allergen and extensin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001665
Physical Location & Seq
Reverse (-)
80676082 .. 80678349
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG590000.1

Sequence Viewer

Length: 1113 bp
ATGTCTTGGTTTCTAACAATATTCTTCCTCATTAGTTTCACATTTAGTACTCCTTCAGAGGCTAACCATGAGAAGAAGCTTCAACCTGCTGTGGTTATAGGTAAAGTTTACTGTGAGACATGTTTCCAAGATGGGTTCTCAAAATCCAGCCACTTCATTTCAGGGGCTTCTGTTGGTGTGGAGTGCAAAGATCAGACTTCATCAAAACCCAGCTTTCAAGCCCAAGTGAAGACAGATAACCAAGGAGTGTTCAAAGTCCAATTGCCTTTCTCAGTGAGCAAACATGTGAAGAAAATTGAAGGGTGTTCTGTGAAGCTGATTCGCAGCAGCGAACCGTATTGTGCTGTGGCCTCAGCAGCAGCTTCATCTTCACTTCACCTCAAATCAAGGAAGCAAGGAACTCACATTTTCTCAGCTGGGTTTTTCACCTTCAAGCCCCTAAACCAACCCAACTTATGTAACCAAAAACCAAGCCTCATCCAAAACAACAAGAAGGAAGTTATCACTAATTCCAAGAAACTCTCATTTTCTCCAACTGAGGATTTGACATTTCCCTCACCAGTTCAAGAACCCAGCTTTCCTGGTGTTCCTTCTCTCCCTAGACTTCCTTATCTTCCACCACTACCACAACTCCCTCCTCTCCCAGGCCTTCCAGGACTCCCAGGTCTTCCAGGAATGGGAATTCCAACTTTACCCCCCATCCCAGGAAAAGTAACAGAGTCCCATTCCAATACCAAAACTACAACTACCTCTTCAAAGACAGAGAGGCACCTAACTGAAGAAGAAAAACTAGCCCGCCCAGATTTTTTCTTCCCACCGAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTTCTGCCAAGCCCACCTCCACTTATCCCAAACCCATTTCAGCCTCCACCAGCACCATTGATTCCAAATTTGCCACCATTGCCTAGCTTGCCACCACTACCAAGTTTAACTCCACCATCTCCACCACCACCATCATTCCCAACAATCCCTCCATTGCCTCCATTCCCATTCCCACCTGTACCTCTCATCCCTGGAATCCCCCCTGCTTCTTCCTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

370

Amino Acids

39.77

Weight (kDa)

9.34

Isoelectric Point (pI)

70.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 32 - 120 3.8e-20 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 768
AciI CCGC 1 cut(s) 796
AcsI RAATTY 2 cut(s) 681, 958
AcuI CTGAAG 2 cut(s) 39, 798
AfaI GTAC 2 cut(s) 49, 1071
AfiI CCNNNNNNNGG 3 cut(s) 644, 677, 704
AflIII ACRYGT 2 cut(s) 119, 283
AgsI TTSAA 7 cut(s) 83, 218, 253, 299, 433, 566, 756
AjnI CCWGG 7 cut(s) 580, 643, 652, 661, 670, 703, 1081
AluBI AGCT 7 cut(s) 79, 213, 316, 362, 416, 576, 978
AluI AGCT 7 cut(s) 79, 213, 316, 362, 416, 576, 978
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 2 cut(s) 348, 646
ApeKI GCWGC 4 cut(s) 324, 327, 356, 359
ApoI RAATTY 2 cut(s) 681, 958
AsuHPI GGTGA 3 cut(s) 368, 418, 549
BanI GGYRCC 1 cut(s) 768
BbsI GAAGAC 2 cut(s) 236, 659
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 4 cut(s) 336, 339, 368, 371
BccI CCATC 6 cut(s) 125, 707, 844, 877, 1015, 1030
BciT130I CCWGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 3 cut(s) 600, 791, 975
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 4 cut(s) 325, 328, 357, 360
BlsI GCNGC 4 cut(s) 326, 329, 358, 361
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
BmiI GGNNCC 1 cut(s) 770
BmrFI CCNGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
BpiI GAAGAC 2 cut(s) 236, 659
Bpu10I CCTNAGC 1 cut(s) 352
BsaJI CCNNGG 5 cut(s) 241, 643, 661, 703, 1081
BsaXI ACNNNNNCTCC 4 cut(s) 615, 645, 1024, 1054
Bsc4I CCNNNNNNNGG 3 cut(s) 644, 677, 704
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 2 cut(s) 968, 1043
BseBI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
BseDI CCNNGG 5 cut(s) 241, 643, 661, 703, 1081
BseGI GGATG 3 cut(s) 477, 699, 1077
BseLI CCNNNNNNNGG 3 cut(s) 644, 677, 704
BseMI GCAATG 2 cut(s) 968, 1043
BseMII CTCAG 4 cut(s) 285, 366, 426, 528
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 4 cut(s) 336, 339, 368, 371
BseYI CCCAGC 3 cut(s) 209, 416, 572
BshFI GGCC 2 cut(s) 350, 648
BshNI GGYRCC 1 cut(s) 768
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 3 cut(s) 644, 677, 704
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 706
BsnI GGCC 2 cut(s) 350, 648
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 796
BspANI GGCC 2 cut(s) 350, 648
BspCNI CTCAG 4 cut(s) 284, 365, 425, 529
BspLI GGNNCC 1 cut(s) 770
BspMI ACCTGC 1 cut(s) 94
BspT107I GGYRCC 1 cut(s) 768
BsrDI GCAATG 2 cut(s) 968, 1043
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 5 cut(s) 241, 643, 661, 703, 1081
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
Bst4CI ACNGT 2 cut(s) 113, 336
Bst6I CTCTTC 2 cut(s) 757, 897
BstC8I GCNNGC 2 cut(s) 796, 980
BstDEI CTNAG 4 cut(s) 271, 352, 412, 537
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 3 cut(s) 477, 699, 1077
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 356, 970, 979
BstNI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
BstNSI RCATGY 2 cut(s) 123, 287
BstSCI CCNGG 7 cut(s) 580, 643, 652, 661, 670, 703, 1081
BstV1I GCAGC 4 cut(s) 336, 339, 368, 371
BstV2I GAAGAC 2 cut(s) 236, 659
BsuRI GGCC 2 cut(s) 350, 648
BtsCI GGATG 3 cut(s) 477, 699, 1077
BtsIMutI CAGTG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 796, 980
Csp6I GTAC 2 cut(s) 48, 1070
CviAII CATG 3 cut(s) 68, 120, 284
CviQI GTAC 2 cut(s) 48, 1070
DdeI CTNAG 4 cut(s) 271, 352, 412, 537
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DrdI GACNNNNNNGTC 1 cut(s) 663
DseDI GACNNNNNNGTC 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 757, 897
EarI CTCTTC 2 cut(s) 757, 897
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 39, 798
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 681
EcoRII CCWGG 7 cut(s) 580, 643, 652, 661, 670, 703, 1081
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 3 cut(s) 71, 123, 287
FaiI YATR 5 cut(s) 69, 98, 121, 285, 457
FaqI GGGAC 1 cut(s) 706
FatI CATG 3 cut(s) 67, 119, 283
FauI CCCGC 1 cut(s) 803
Fnu4HI GCNGC 4 cut(s) 325, 328, 357, 360
FokI GGATG 3 cut(s) 464, 686, 1064
Fsp4HI GCNGC 4 cut(s) 325, 328, 357, 360
FspBI CTAG 3 cut(s) 600, 791, 975
GluI GCNGC 4 cut(s) 325, 328, 357, 360
GsaI CCCAGC 3 cut(s) 213, 420, 576
HaeIII GGCC 2 cut(s) 350, 648
Hin1II CATG 3 cut(s) 71, 123, 287
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 5 cut(s) 319, 657, 719, 952, 1086
HphI GGTGA 3 cut(s) 368, 418, 549
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 58, 195
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 6 cut(s) 63, 293, 439, 487, 600, 659
HpyCH4III ACNGT 2 cut(s) 113, 336
HpyCH4V TGCA 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 970, 979
HpyF3I CTNAG 4 cut(s) 271, 352, 412, 537
Hsp92II CATG 3 cut(s) 71, 123, 287
Kzo9I GATC 1 cut(s) 190
Lsp1109I GCAGC 4 cut(s) 336, 339, 368, 371
MaeI CTAG 3 cut(s) 600, 791, 975
MaeIII GTNAC 2 cut(s) 458, 712
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MfeI CAATTG 1 cut(s) 260
MluCI AATT 7 cut(s) 260, 294, 508, 681, 840, 873, 958
MlyI GAGTC 2 cut(s) 651, 728
MmeI TCCRAC 2 cut(s) 557, 710
MseI TTAA 1 cut(s) 998
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
MwoI GCNNNNNNNGC 3 cut(s) 356, 970, 979
NdeII GATC 1 cut(s) 190
NlaIII CATG 3 cut(s) 71, 123, 287
NlaIV GGNNCC 1 cut(s) 770
NspI RCATGY 2 cut(s) 123, 287
PceI AGGCCT 1 cut(s) 648
PciI ACATGT 2 cut(s) 119, 283
PfeI GAWTC 3 cut(s) 319, 952, 1086
PfoI TCCNGGA 2 cut(s) 652, 670
PkrI GCNGC 4 cut(s) 326, 329, 358, 361
PleI GAGTC 2 cut(s) 651, 727
PpsI GAGTC 2 cut(s) 651, 727
PscI ACATGT 2 cut(s) 119, 283
Psp6I CCWGG 7 cut(s) 580, 643, 652, 661, 670, 703, 1081
PspFI CCCAGC 3 cut(s) 209, 416, 572
PspGI CCWGG 7 cut(s) 580, 643, 652, 661, 670, 703, 1081
PspN4I GGNNCC 1 cut(s) 770
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 49, 1071
RsaNI GTAC 2 cut(s) 48, 1070
SaqAI TTAA 1 cut(s) 998
SatI GCNGC 4 cut(s) 325, 328, 357, 360
Sau3AI GATC 1 cut(s) 190
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 2 cut(s) 651, 728
ScrFI CCNGG 7 cut(s) 582, 645, 654, 663, 672, 705, 1083
Sse9I AATT 7 cut(s) 260, 294, 508, 681, 840, 873, 958
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 1 cut(s) 796
SspI AATATT 1 cut(s) 21
SspMI CTAG 3 cut(s) 600, 791, 975
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 7 cut(s) 580, 643, 652, 661, 670, 703, 1081
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 113, 336
TasI AATT 7 cut(s) 260, 294, 508, 681, 840, 873, 958
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 3 cut(s) 319, 952, 1086
Tru1I TTAA 1 cut(s) 998
Tru9I TTAA 1 cut(s) 998
TscAI CASTG 1 cut(s) 279
TseI GCWGC 4 cut(s) 324, 327, 356, 359
TspDTI ATGAA 3 cut(s) 145, 189, 354
TspRI CASTG 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 2 cut(s) 681, 958
XceI RCATGY 2 cut(s) 123, 287
XspI CTAG 3 cut(s) 600, 791, 975
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.