Rh2DG600200

Pollen Ole e 1 allergen and extensin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001665
Physical Location & Seq
Reverse (-)
83209534 .. 83211818
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG600200.1

Sequence Viewer

Length: 1140 bp
ATGTCTTGGTTTCTAACAATATTCTTCCTCATTAGTTTCACATTTAGTACTCCTTCAGAGGCTAACCATGAGAAGAAGCTTCAACCTGCTGTGGTTATAGGCAAAGTTTACTGTGAGACATGTTTCCAAGATGGGTTCTCAAAATCCAGCCACTTCATTTCAGGGGCTTCTGTTGGTGTGGAGTGCAAAGATCAGACTTCATCAAAACCCAGCTTTCAAGCCCAAGTGAAGACAGATAACCAAGGAGTGTTCAAAGTCCAATTGCCTTTCTCAGTGAGCAAACATGTGAAGAAAATTGAAGGGTGTTCTGTGAAGCTAATTCGCAGCAGCGAACCGTATTGTGCTGTGGCCTCAGCAGCATCTTCATCTTCACTTCACCTCAAATCAAGGAAGCAAGGAACTCACATTTTCTCAGCTGGGTTTTTCACCTTCAAGCCCCTAAACCAACCCAACCTATGTAACCAAAAACCAAGCCTCGTCCAAAATAACAAGAAGGAAGTTATCACTAATTCCAAGAAACTCTCATTTTCTCCAACTGAGGATTTGACATTTCCCTCACCAGTTCAAGAACCCAGCTTTCCTGGTGTTCCTTCTCTCCCTAGACTTCCTTATCTTCCACCATTACCACAACTCCCTCCTCTCCCAGGCCTGCCAGGACTCCCAGGTCTTCCAGGACTTCCAGGAATGGGAATTCCAACTTTACCCCCCATCCCAGGAAAAGTAACAGAGTCCCATTCCAATACCAAAACTACAACTACCTCTTCAAAGACAGAGAAGCACCTAACTGAAGAAGAAAAACTAGCCCGCCCAGATTTTTTCTTCCCACCGAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTTCTGCCAAGCCCACCTCCACTTATCCCAAACCCATTTCAGCCTCCACCAGCACCATTGATTCCAAATTTGCCACCATTGCCTAGCTTGCCACCACTGCCTAGCTTGCCACCACTACCAAGTTTAACTCCACCATCTCCACCACCACCATCATTCCCAACAATCCCTCCATTGCCTCCATTCCCATTCCCACCTGTACCTCTCATCCCTGGAATCCCCCCTGCTTCTTCCTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

379

Amino Acids

40.62

Weight (kDa)

9.33

Isoelectric Point (pI)

71.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 32 - 121 3.8e-20 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 94
AciI CCGC 1 cut(s) 805
AcsI RAATTY 2 cut(s) 690, 967
AcuI CTGAAG 2 cut(s) 39, 807
AfaI GTAC 2 cut(s) 49, 1098
AfiI CCNNNNNNNGG 3 cut(s) 644, 686, 713
AflIII ACRYGT 2 cut(s) 119, 283
AgsI TTSAA 7 cut(s) 83, 218, 253, 299, 433, 566, 765
AjnI CCWGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1108
AluBI AGCT 7 cut(s) 79, 213, 316, 416, 576, 987, 1005
AluI AGCT 7 cut(s) 79, 213, 316, 416, 576, 987, 1005
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 2 cut(s) 348, 646
ApeKI GCWGC 3 cut(s) 324, 327, 356
ApoI RAATTY 2 cut(s) 690, 967
AsuHPI GGTGA 3 cut(s) 368, 418, 549
BbsI GAAGAC 2 cut(s) 236, 659
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 3 cut(s) 336, 339, 368
BccI CCATC 6 cut(s) 125, 716, 853, 886, 1042, 1057
BciT130I CCWGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 4 cut(s) 600, 800, 984, 1002
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 3 cut(s) 325, 328, 357
BlsI GCNGC 3 cut(s) 326, 329, 358
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
BmrFI CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
BmsI GCATC 1 cut(s) 368
BpiI GAAGAC 2 cut(s) 236, 659
Bpu10I CCTNAGC 1 cut(s) 352
BsaJI CCNNGG 5 cut(s) 241, 643, 661, 712, 1108
BsaXI ACNNNNNCTCC 4 cut(s) 615, 645, 1051, 1081
Bsc4I CCNNNNNNNGG 3 cut(s) 644, 686, 713
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 2 cut(s) 977, 1070
BseBI CCWGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
BseDI CCNNGG 5 cut(s) 241, 643, 661, 712, 1108
BseGI GGATG 2 cut(s) 708, 1104
BseLI CCNNNNNNNGG 3 cut(s) 644, 686, 713
BseMI GCAATG 2 cut(s) 977, 1070
BseMII CTCAG 4 cut(s) 285, 366, 426, 528
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 3 cut(s) 336, 339, 368
BseYI CCCAGC 3 cut(s) 209, 416, 572
BshFI GGCC 2 cut(s) 350, 648
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 3 cut(s) 644, 686, 713
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 715
BsnI GGCC 2 cut(s) 350, 648
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 805
BspANI GGCC 2 cut(s) 350, 648
BspCNI CTCAG 4 cut(s) 284, 365, 425, 529
BspMI ACCTGC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 977, 1070
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 5 cut(s) 241, 643, 661, 712, 1108
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
Bst4CI ACNGT 2 cut(s) 113, 336
Bst6I CTCTTC 2 cut(s) 766, 906
BstC8I GCNNGC 4 cut(s) 650, 805, 989, 1007
BstDEI CTNAG 4 cut(s) 271, 352, 412, 537
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 2 cut(s) 708, 1104
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 5 cut(s) 356, 979, 988, 997, 1006
BstNI CCWGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
BstNSI RCATGY 2 cut(s) 123, 287
BstSCI CCNGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1108
BstV1I GCAGC 3 cut(s) 336, 339, 368
BstV2I GAAGAC 2 cut(s) 236, 659
BsuRI GGCC 2 cut(s) 350, 648
BtsCI GGATG 2 cut(s) 708, 1104
BtsI GCAGTG 1 cut(s) 995
BtsIMutI CAGTG 2 cut(s) 279, 995
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 4 cut(s) 650, 805, 989, 1007
Csp6I GTAC 2 cut(s) 48, 1097
CviAII CATG 3 cut(s) 68, 120, 284
CviQI GTAC 2 cut(s) 48, 1097
DdeI CTNAG 4 cut(s) 271, 352, 412, 537
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DrdI GACNNNNNNGTC 1 cut(s) 663
DseDI GACNNNNNNGTC 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 766, 906
EarI CTCTTC 2 cut(s) 766, 906
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 39, 807
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 690
EcoRII CCWGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1108
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 3 cut(s) 71, 123, 287
FaiI YATR 5 cut(s) 69, 98, 121, 285, 457
FaqI GGGAC 1 cut(s) 715
FatI CATG 3 cut(s) 67, 119, 283
FauI CCCGC 1 cut(s) 812
Fnu4HI GCNGC 3 cut(s) 325, 328, 357
FokI GGATG 2 cut(s) 695, 1091
Fsp4HI GCNGC 3 cut(s) 325, 328, 357
FspBI CTAG 4 cut(s) 600, 800, 984, 1002
GluI GCNGC 3 cut(s) 325, 328, 357
GsaI CCCAGC 3 cut(s) 213, 420, 576
HaeIII GGCC 2 cut(s) 350, 648
Hin1II CATG 3 cut(s) 71, 123, 287
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 4 cut(s) 657, 728, 961, 1113
HphI GGTGA 3 cut(s) 368, 418, 549
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 58, 195
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 5 cut(s) 63, 293, 439, 487, 600
HpyCH4III ACNGT 2 cut(s) 113, 336
HpyCH4V TGCA 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 979, 988, 997, 1006
HpyF3I CTNAG 4 cut(s) 271, 352, 412, 537
Hsp92II CATG 3 cut(s) 71, 123, 287
Kzo9I GATC 1 cut(s) 190
Lsp1109I GCAGC 3 cut(s) 336, 339, 368
LweI GCATC 1 cut(s) 368
MaeI CTAG 4 cut(s) 600, 800, 984, 1002
MaeIII GTNAC 2 cut(s) 458, 721
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MfeI CAATTG 1 cut(s) 260
MluCI AATT 8 cut(s) 260, 294, 318, 508, 690, 849, 882, 967
MlyI GAGTC 2 cut(s) 651, 737
MmeI TCCRAC 2 cut(s) 557, 719
MseI TTAA 1 cut(s) 1025
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
MwoI GCNNNNNNNGC 5 cut(s) 356, 979, 988, 997, 1006
NdeII GATC 1 cut(s) 190
NlaIII CATG 3 cut(s) 71, 123, 287
NspI RCATGY 2 cut(s) 123, 287
PceI AGGCCT 1 cut(s) 648
PciI ACATGT 2 cut(s) 119, 283
PfeI GAWTC 2 cut(s) 961, 1113
PfoI TCCNGGA 2 cut(s) 670, 679
PkrI GCNGC 3 cut(s) 326, 329, 358
PleI GAGTC 2 cut(s) 651, 736
PpsI GAGTC 2 cut(s) 651, 736
PscI ACATGT 2 cut(s) 119, 283
Psp6I CCWGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1108
PspFI CCCAGC 3 cut(s) 209, 416, 572
PspGI CCWGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1108
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 49, 1098
RsaNI GTAC 2 cut(s) 48, 1097
SaqAI TTAA 1 cut(s) 1025
SatI GCNGC 3 cut(s) 325, 328, 357
Sau3AI GATC 1 cut(s) 190
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 2 cut(s) 651, 737
ScrFI CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1110
SfaNI GCATC 1 cut(s) 368
Sse9I AATT 8 cut(s) 260, 294, 318, 508, 690, 849, 882, 967
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 1 cut(s) 805
SspI AATATT 1 cut(s) 21
SspMI CTAG 4 cut(s) 600, 800, 984, 1002
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1108
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 113, 336
TasI AATT 8 cut(s) 260, 294, 318, 508, 690, 849, 882, 967
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 2 cut(s) 961, 1113
Tru1I TTAA 1 cut(s) 1025
Tru9I TTAA 1 cut(s) 1025
TscAI CASTG 2 cut(s) 279, 1002
TseI GCWGC 3 cut(s) 324, 327, 356
TspDTI ATGAA 3 cut(s) 145, 189, 354
TspRI CASTG 2 cut(s) 279, 1002
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 2 cut(s) 690, 967
XceI RCATGY 2 cut(s) 123, 287
XspI CTAG 4 cut(s) 600, 800, 984, 1002
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.