MD09G1075000.v1.1

Pollen Ole e 1 allergen and extensin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001665
Physical Location & Seq
Forward (+)
5226047 .. 5228362
2316 bp
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UTR
Exon/CDS
Intron
MD09G1075000.v1.1.491

Sequence Viewer

Length: 768 bp
ATGAGGAGATTCCAGCCTTCTGCAGTAGTTGTGGGAACTGTCTACTGTGACACATGTTTCCAAGAGGATTTCTCAAATACCAGCCATTTCATTTCAGGGGCTTCTGTTGGTGTGGAATGCAAAGATGGGACTTTAACAAAACAAAGTTTCAACGCACAAGTGAAAACAAACAGCCATGGGCAGTTCAAAGTCCATCTGCCCTTCTCAGTGAGCAAAGATGTCAAGAAAATTGAAGGATGCTCCGTGAAACTAATCCGCAGCAGTGAACCCTACTGTGCTGTGGCCTCATCAGCCACCTCATCTTCCCTACACCTCAAGGCAACAAAACAAGGGACTTACATATTCTCTGCTGGTTTCTTCACCTTCAAGCCTCTCAAGCAACCCAGCTTATGCAACCAGAAACCCAACATTCAGAATTCCAAGAAATTAAATTCCCAGAAGTTCTCAGAGATGGCATTCCCATCCTCCTCTCAGGTGGACCCCAAAATGCCTGATCCCTCTCTCAACCAATCTGATCTCCTAGGTCTCCCAGGTCTCCCAGGTCTCCCAGGTCTCCCAGGACTCCCAGGTCTCCCAGGTCTCCCAGGACTCCCAGGACTCCCAGGACTCCCAGGTCTCCCAGGCCTCCCAGGACTTCCAGGCCTGCCCCTTGCTGCTGAAAAAACTTCATCCAAGTCCTCCAACTCCAAAACTTCAAGGAATGGGCAACTAGGTGATAATAAAGAAGTAGCCCATCCAGATTCACCACCAGCAAGTTCAGCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

256

Amino Acids

26.74

Weight (kDa)

9.3

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 10 - 99 8e-21 Pollen protein Ole e 1 like
Collagen PF01391 176 - 214 9e-08 Collagen triple helix repeat (20 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 567, 612
AccI GTMKAC 1 cut(s) 42
AciI CCGC 1 cut(s) 256
AclWI GGATC 1 cut(s) 488
AcsI RAATTY 2 cut(s) 415, 430
AflIII ACRYGT 1 cut(s) 53
AgsI TTSAA 5 cut(s) 151, 187, 233, 367, 696
AluBI AGCT 1 cut(s) 387
AluI AGCT 1 cut(s) 387
Alw26I GTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
AlwI GGATC 1 cut(s) 488
AoxI GGCC 3 cut(s) 282, 622, 640
ApeKI GCWGC 2 cut(s) 258, 653
ApoI RAATTY 2 cut(s) 415, 430
AspA2I CCTAGG 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 478
AsuHPI GGTGA 3 cut(s) 352, 725, 735
AvaII GGWCC 1 cut(s) 478
AvrII CCTAGG 1 cut(s) 520
BbvI GCAGC 2 cut(s) 270, 640
BccI CCATC 5 cut(s) 119, 201, 445, 469, 741
BcoDI GTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
BfaI CTAG 2 cut(s) 521, 710
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 2 cut(s) 259, 654
BlnI CCTAGG 1 cut(s) 520
BlsI GCNGC 2 cut(s) 260, 655
Bme18I GGWCC 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 480
BmsI GCATC 1 cut(s) 227
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BpuEI CTTGAG 2 cut(s) 299, 359
BsaI GGTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
BseGI GGATG 4 cut(s) 242, 461, 668, 733
BseMII CTCAG 3 cut(s) 219, 459, 485
BseRI GAGGAG 2 cut(s) 19, 457
BseXI GCAGC 2 cut(s) 270, 640
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 3 cut(s) 284, 624, 642
BslFI GGGAC 2 cut(s) 142, 346
BsmAI GTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
BsmFI GGGAC 2 cut(s) 142, 346
BsmI GAATGC 2 cut(s) 122, 455
BsnI GGCC 3 cut(s) 284, 624, 642
Bso31I GGTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
Bsp143I GATC 2 cut(s) 493, 514
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 256
BspANI GGCC 3 cut(s) 284, 624, 642
BspCNI CTCAG 3 cut(s) 218, 458, 484
BspLI GGNNCC 1 cut(s) 480
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 1 cut(s) 488
BspTNI GGTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
BssMI GATC 2 cut(s) 493, 514
BssT1I CCWWGG 2 cut(s) 175, 520
Bst4CI ACNGT 3 cut(s) 40, 47, 275
BstC8I GCNNGC 1 cut(s) 644
BstDEI CTNAG 3 cut(s) 205, 445, 471
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 4 cut(s) 242, 461, 668, 733
BstKTI GATC 2 cut(s) 496, 517
BstMAI GTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
BstMBI GATC 2 cut(s) 493, 514
BstMWI GCNNNNNNNGC 3 cut(s) 290, 376, 758
BstNSI RCATGY 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 270, 640
BsuRI GGCC 3 cut(s) 284, 624, 642
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 4 cut(s) 242, 461, 668, 733
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 213, 268
Cac8I GCNNGC 1 cut(s) 644
Cfr13I GGNCC 1 cut(s) 478
CviAII CATG 3 cut(s) 54, 176, 765
DdeI CTNAG 3 cut(s) 205, 445, 471
DpnI GATC 2 cut(s) 495, 516
DpnII GATC 2 cut(s) 493, 514
DrdI GACNNNNNNGTC 2 cut(s) 567, 612
DseDI GACNNNNNNGTC 2 cut(s) 567, 612
Eco130I CCWWGG 2 cut(s) 175, 520
Eco147I AGGCCT 2 cut(s) 624, 642
Eco31I GGTCTC 7 cut(s) 530, 539, 548, 557, 575, 584, 620
Eco47I GGWCC 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 415
EcoT14I CCWWGG 2 cut(s) 175, 520
ErhI CCWWGG 2 cut(s) 175, 520
FaeI CATG 3 cut(s) 57, 179, 768
FaiI YATR 5 cut(s) 55, 177, 341, 391, 766
FaqI GGGAC 2 cut(s) 142, 346
FatI CATG 3 cut(s) 53, 175, 764
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 259, 654
FokI GGATG 4 cut(s) 249, 448, 655, 720
Fsp4HI GCNGC 2 cut(s) 259, 654
FspBI CTAG 2 cut(s) 521, 710
GluI GCNGC 2 cut(s) 259, 654
GsaI CCCAGC 1 cut(s) 387
HaeIII GGCC 3 cut(s) 284, 624, 642
Hin1II CATG 3 cut(s) 57, 179, 768
HinfI GANTC 6 cut(s) 9, 561, 588, 597, 606, 740
HphI GGTGA 3 cut(s) 352, 725, 735
Hpy166II GTNNAC 3 cut(s) 43, 266, 478
Hpy188I TCNGA 3 cut(s) 414, 448, 514
Hpy188III TCNNGA 2 cut(s) 223, 737
Hpy8I GTNNAC 3 cut(s) 43, 266, 478
HpyAV CCTTC 4 cut(s) 27, 211, 227, 373
HpyCH4III ACNGT 3 cut(s) 40, 47, 275
HpyCH4V TGCA 3 cut(s) 23, 120, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 376, 758
HpyF3I CTNAG 3 cut(s) 205, 445, 471
Hsp92II CATG 3 cut(s) 57, 179, 768
Kzo9I GATC 2 cut(s) 493, 514
LmnI GCTCC 1 cut(s) 245
Lsp1109I GCAGC 2 cut(s) 270, 640
LweI GCATC 1 cut(s) 227
MaeI CTAG 2 cut(s) 521, 710
MaeIII GTNAC 1 cut(s) 47
MalI GATC 2 cut(s) 495, 516
MboI GATC 2 cut(s) 493, 514
MboII GAAGA 2 cut(s) 294, 349
MluCI AATT 4 cut(s) 228, 415, 425, 430
MlyI GAGTC 4 cut(s) 555, 582, 591, 600
MmeI TCCRAC 1 cut(s) 705
MseI TTAA 2 cut(s) 134, 428
Mva1269I GAATGC 2 cut(s) 122, 455
MwoI GCNNNNNNNGC 3 cut(s) 290, 376, 758
NcoI CCATGG 1 cut(s) 175
NdeII GATC 2 cut(s) 493, 514
NlaIII CATG 3 cut(s) 57, 179, 768
NlaIV GGNNCC 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 47
NspI RCATGY 1 cut(s) 57
PceI AGGCCT 2 cut(s) 624, 642
PciI ACATGT 1 cut(s) 53
PctI GAATGC 2 cut(s) 122, 455
PfeI GAWTC 2 cut(s) 9, 740
PkrI GCNGC 2 cut(s) 260, 655
PleI GAGTC 4 cut(s) 555, 582, 591, 600
PpsI GAGTC 4 cut(s) 555, 582, 591, 600
PscI ACATGT 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 383
PspN4I GGNNCC 1 cut(s) 480
PspPI GGNCC 1 cut(s) 478
PstI CTGCAG 1 cut(s) 25
SaqAI TTAA 2 cut(s) 134, 428
SatI GCNGC 2 cut(s) 259, 654
Sau3AI GATC 2 cut(s) 493, 514
Sau96I GGNCC 1 cut(s) 478
SchI GAGTC 4 cut(s) 555, 582, 591, 600
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 478
SmlI CTYRAG 2 cut(s) 314, 374
SmoI CTYRAG 2 cut(s) 314, 374
Sse9I AATT 4 cut(s) 228, 415, 425, 430
SseBI AGGCCT 2 cut(s) 624, 642
SsiI CCGC 1 cut(s) 256
SspMI CTAG 2 cut(s) 521, 710
StuI AGGCCT 2 cut(s) 624, 642
StyI CCWWGG 2 cut(s) 175, 520
TaaI ACNGT 3 cut(s) 40, 47, 275
TasI AATT 4 cut(s) 228, 415, 425, 430
TfiI GAWTC 2 cut(s) 9, 740
Tru1I TTAA 2 cut(s) 134, 428
Tru9I TTAA 2 cut(s) 134, 428
TscAI CASTG 2 cut(s) 213, 268
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 2 cut(s) 258, 653
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 2 cut(s) 79, 657
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 2 cut(s) 213, 268
VpaK11BI GGWCC 1 cut(s) 478
XapI RAATTY 2 cut(s) 415, 430
XceI RCATGY 1 cut(s) 57
XmaJI CCTAGG 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 2 cut(s) 521, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.