MD09G1075500.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001665
Physical Location & Seq
Forward (+)
5312905 .. 5314783
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1075500.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGTCTTGGCTTACAGTTATCTTTCTGGTTATTACTCTTACATTTCATACTCCTTCAGAGGCTAGCCATGAGAAGAACGATCCTTCTGCAGTTGTTGTAGGCACTGTCTACTGTGACACATGTTTCCAAGAGGATTTCTCACATACCAGCCATTTCATTTCAGGGGCTTTTGTTGGTGTGGAATGCAATGATGGGAGTTCAACGAAACCAAGTTTTAAGGCACAAGTGAAAACAAATAGCCATGGACAATTCAAAGTCCATCTGCCCTTCTCAGTGAGCAAACATGTGAAGAAAATTGAAGGATGCTCTGTGAAACTAATCAGCAGCAGTGAACCCTACTGTGCTGTTGCCTCATCAGCCACCTCATCTTCCCTACACCTCAAGGCAAGAAAGCAAGGGACCCACATATTCTCTGCTGGTTTCTTCACCTTCAAGCCTCTCAAGCAACCCGGTTTATGCAACCAGAAACCCAGCATTCAGAATTCCAAGAAATTAAATTCCCAGAAGTTCTCATTCCCTCCTACTGATGAGCTGGCATTCCCATCCCCCTCTCAGGCGGACCCCACAATGACTGATCTCCCTCCCGTCCCTAGGATCCCTTATCTTCCACCACTGCCACAGCTTCCTCCTCTCTCTCCCACCCCAACTGGTCTCCCAGGAGGTCTCCCAGGACTCCCAGGTCTCCCAGGCCTCCCAGGCCTCCCTGGACTCCCAGGACTCCCAGGACTCCCAGGACTCCCAGGCCTCCCAGGTCTCCCAGGCCTCCCAGGACTTCCTGGAATTCCAGGTTCTCCCCTTGCTGCTGGAAAAACTTCATCTGGGTCGTCCAACTCCAAAACTTCAAAGAATGGGCAACTAGGTGATAATAAAGAAGTAGTCCATCCAGATACTTTCTTTCCACCAATTATCCCTAATCCATTTCAGCCCCCACCGGCTCCATTAATTCCTAATTTGCCTCCTCTTCCAGGTTCTCCACCAGCAGGTCCCGCACCATTGATTCCCAACCTGCCACCTATACCTGGTTTCACTCCATCGCCGTCACCGCCACCACCAACCCTCCCATTCCTCCCTCCATTCCCATTCCCACTCCCACCTACGCCTCGGATCCCTGGAATCCCCCCAGCTTCTTCTTCAAAACAAAGCACTTCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

385

Amino Acids

39.91

Weight (kDa)

9.19

Isoelectric Point (pI)

61.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 32 - 121 9.4e-21 Pollen protein Ole e 1 like
Collagen PF01391 221 - 269 7.8e-06 Collagen triple helix repeat (20 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 678
Acc36I ACCTGC 2 cut(s) 971, 1014
AccI GTMKAC 1 cut(s) 108
AciI CCGC 3 cut(s) 557, 987, 1043
AclWI GGATC 5 cut(s) 74, 589, 602, 1099, 1112
AcsI RAATTY 3 cut(s) 481, 496, 780
AcuI CTGAAG 1 cut(s) 39
AfiI CCNNNNNNNGG 5 cut(s) 553, 591, 965, 980, 1019
AflIII ACRYGT 2 cut(s) 119, 283
AgsI TTSAA 6 cut(s) 201, 253, 299, 433, 843, 1134
AluBI AGCT 3 cut(s) 532, 622, 1124
AluI AGCT 3 cut(s) 532, 622, 1124
Alw26I GTCTC 4 cut(s) 656, 668, 686, 758
AlwI GGATC 5 cut(s) 74, 589, 602, 1099, 1112
AoxI GGCC 4 cut(s) 688, 697, 742, 760
ApeKI GCWGC 2 cut(s) 324, 800
ApoI RAATTY 3 cut(s) 481, 496, 780
AseI ATTAAT 1 cut(s) 941
Asp700I GAANNNNTTC 1 cut(s) 811
AspA2I CCTAGG 1 cut(s) 590
AspS9I GGNCC 3 cut(s) 399, 559, 983
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 4 cut(s) 418, 872, 1032, 1140
AsuNHI GCTAGC 1 cut(s) 62
AvaII GGWCC 3 cut(s) 399, 559, 983
AvrII CCTAGG 1 cut(s) 590
BamHI GGATCC 2 cut(s) 594, 1104
BbvI GCAGC 2 cut(s) 336, 787
BccI CCATC 5 cut(s) 185, 267, 550, 888, 1039
BceAI ACGGC 1 cut(s) 1021
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcnI CCSGG 1 cut(s) 450
BcoDI GTCTC 4 cut(s) 656, 668, 686, 758
BfaI CTAG 3 cut(s) 63, 591, 857
BfmI CTRYAG 1 cut(s) 87
BfuAI ACCTGC 2 cut(s) 971, 1014
BglI GCCNNNNNGGC 1 cut(s) 696
BisI GCNGC 2 cut(s) 325, 801
BlnI CCTAGG 1 cut(s) 590
BlsI GCNGC 2 cut(s) 326, 802
Bme18I GGWCC 3 cut(s) 399, 559, 983
BmgT120I GGNCC 3 cut(s) 399, 559, 983
BmiI GGNNCC 7 cut(s) 400, 401, 561, 596, 936, 985, 1106
BmsI GCATC 1 cut(s) 293
BmtI GCTAGC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpuEI CTTGAG 2 cut(s) 365, 425
BpuMI CCSGG 1 cut(s) 450
BsaI GGTCTC 4 cut(s) 656, 668, 686, 758
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 5 cut(s) 553, 591, 965, 980, 1019
Bse118I RCCGGY 1 cut(s) 931
Bse1I ACTGG 1 cut(s) 652
Bse3DI GCAATG 1 cut(s) 193
BseGI GGATG 3 cut(s) 308, 542, 880
BseLI CCNNNNNNNGG 5 cut(s) 553, 591, 965, 980, 1019
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 2 cut(s) 285, 566
BseNI ACTGG 1 cut(s) 652
BseRI GAGGAG 2 cut(s) 618, 948
BseXI GCAGC 2 cut(s) 336, 787
BseYI CCCAGC 2 cut(s) 470, 1120
BshFI GGCC 4 cut(s) 690, 699, 744, 762
BsiSI CCGG 2 cut(s) 450, 932
BslFI GGGAC 3 cut(s) 412, 572, 969
BslI CCNNNNNNNGG 5 cut(s) 553, 591, 965, 980, 1019
BsmAI GTCTC 4 cut(s) 656, 668, 686, 758
BsmFI GGGAC 3 cut(s) 412, 572, 969
BsmI GAATGC 3 cut(s) 188, 474, 536
BsnI GGCC 4 cut(s) 690, 699, 744, 762
Bso31I GGTCTC 4 cut(s) 656, 668, 686, 758
Bsp143I GATC 4 cut(s) 79, 574, 594, 1104
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 3 cut(s) 557, 987, 1043
BspANI GGCC 4 cut(s) 690, 699, 744, 762
BspCNI CTCAG 2 cut(s) 284, 565
BspLI GGNNCC 7 cut(s) 400, 401, 561, 596, 936, 985, 1106
BspMAI CTGCAG 1 cut(s) 91
BspMI ACCTGC 2 cut(s) 971, 1014
BspOI GCTAGC 1 cut(s) 66
BspPI GGATC 5 cut(s) 74, 589, 602, 1099, 1112
BspTNI GGTCTC 4 cut(s) 656, 668, 686, 758
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 931
BsrI ACTGG 1 cut(s) 652
BssAI RCCGGY 1 cut(s) 931
BssMI GATC 4 cut(s) 79, 574, 594, 1104
BssT1I CCWWGG 2 cut(s) 241, 590
Bst4CI ACNGT 4 cut(s) 16, 106, 113, 341
Bst6I CTCTTC 1 cut(s) 966
BstC8I GCNNGC 2 cut(s) 64, 534
BstDEI CTNAG 2 cut(s) 271, 552
BstDSI CCRYGG 1 cut(s) 241
BstENI CCTNNNNNAGG 1 cut(s) 963
BstF5I GGATG 3 cut(s) 308, 542, 880
BstKTI GATC 4 cut(s) 82, 577, 597, 1107
BstMAI GTCTC 4 cut(s) 656, 668, 686, 758
BstMBI GATC 4 cut(s) 79, 574, 594, 1104
BstMWI GCNNNNNNNGC 5 cut(s) 356, 442, 696, 986, 1042
BstNSI RCATGY 2 cut(s) 123, 287
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 336, 787
BstX2I RGATCY 2 cut(s) 594, 1104
BstYI RGATCY 2 cut(s) 594, 1104
BsuRI GGCC 4 cut(s) 690, 699, 744, 762
BtgI CCRYGG 1 cut(s) 241
BtgZI GCGATG 1 cut(s) 1017
BtsCI GGATG 3 cut(s) 308, 542, 880
BtsI GCAGTG 2 cut(s) 334, 611
BtsIMutI CAGTG 4 cut(s) 102, 279, 334, 611
BveI ACCTGC 2 cut(s) 971, 1014
Cac8I GCNNGC 2 cut(s) 64, 534
Cfr10I RCCGGY 1 cut(s) 931
Cfr13I GGNCC 3 cut(s) 399, 559, 983
CsiI ACCWGGT 1 cut(s) 1018
CviAII CATG 4 cut(s) 68, 120, 242, 284
DdeI CTNAG 2 cut(s) 271, 552
DpnI GATC 4 cut(s) 81, 576, 596, 1106
DpnII GATC 4 cut(s) 79, 574, 594, 1104
DrdI GACNNNNNNGTC 1 cut(s) 678
DseDI GACNNNNNNGTC 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
EciI GGCGGA 1 cut(s) 572
Eco130I CCWWGG 2 cut(s) 241, 590
Eco147I AGGCCT 4 cut(s) 690, 699, 744, 762
Eco31I GGTCTC 4 cut(s) 656, 668, 686, 758
Eco47I GGWCC 3 cut(s) 399, 559, 983
Eco57I CTGAAG 1 cut(s) 39
EcoNI CCTNNNNNAGG 1 cut(s) 963
EcoO109I RGGNCCY 2 cut(s) 399, 983
EcoRI GAATTC 2 cut(s) 481, 780
EcoT14I CCWWGG 2 cut(s) 241, 590
ErhI CCWWGG 2 cut(s) 241, 590
FaeI CATG 4 cut(s) 71, 123, 245, 287
FaiI YATR 9 cut(s) 48, 69, 121, 144, 243, 285, 407, 457, 1016
FaqI GGGAC 3 cut(s) 412, 572, 969
FatI CATG 4 cut(s) 67, 119, 241, 283
FauI CCCGC 1 cut(s) 994
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 325, 801
FokI GGATG 3 cut(s) 315, 529, 867
Fsp4HI GCNGC 2 cut(s) 325, 801
FspBI CTAG 3 cut(s) 63, 591, 857
GluI GCNGC 2 cut(s) 325, 801
GsaI CCCAGC 2 cut(s) 474, 1124
HaeIII GGCC 4 cut(s) 690, 699, 744, 762
HapII CCGG 2 cut(s) 450, 932
Hin1II CATG 4 cut(s) 71, 123, 245, 287
HinfI GANTC 7 cut(s) 672, 708, 717, 726, 735, 997, 1113
HpaII CCGG 2 cut(s) 450, 932
HphI GGTGA 4 cut(s) 418, 872, 1032, 1140
Hpy166II GTNNAC 2 cut(s) 109, 332
Hpy188I TCNGA 3 cut(s) 58, 480, 1104
Hpy188III TCNNGA 1 cut(s) 884
Hpy8I GTNNAC 2 cut(s) 109, 332
HpyAV CCTTC 5 cut(s) 63, 93, 277, 293, 439
HpyCH4III ACNGT 4 cut(s) 16, 106, 113, 341
HpyCH4V TGCA 3 cut(s) 89, 186, 459
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 442, 696, 986, 1042
HpyF3I CTNAG 2 cut(s) 271, 552
Hsp92II CATG 4 cut(s) 71, 123, 245, 287
KflI GGGWCCC 1 cut(s) 399
Kzo9I GATC 4 cut(s) 79, 574, 594, 1104
LmnI GCTCC 1 cut(s) 940
Lsp1109I GCAGC 2 cut(s) 336, 787
LweI GCATC 1 cut(s) 293
MabI ACCWGGT 1 cut(s) 1018
MaeI CTAG 3 cut(s) 63, 591, 857
MaeIII GTNAC 2 cut(s) 113, 1038
MalI GATC 4 cut(s) 81, 576, 596, 1106
MboI GATC 4 cut(s) 79, 574, 594, 1104
MboII GAAGA 8 cut(s) 85, 301, 360, 415, 596, 953, 1119, 1122
MflI RGATCY 2 cut(s) 594, 1104
MluCI AATT 9 cut(s) 248, 294, 481, 491, 496, 780, 903, 942, 949
MlyI GAGTC 5 cut(s) 666, 702, 711, 720, 729
MmeI TCCRAC 1 cut(s) 852
MroXI GAANNNNTTC 1 cut(s) 811
MseI TTAA 3 cut(s) 216, 494, 941
MspI CCGG 2 cut(s) 450, 932
Mva1269I GAATGC 3 cut(s) 188, 474, 536
MwoI GCNNNNNNNGC 5 cut(s) 356, 442, 696, 986, 1042
NciI CCSGG 1 cut(s) 450
NcoI CCATGG 1 cut(s) 241
NdeII GATC 4 cut(s) 79, 574, 594, 1104
NheI GCTAGC 1 cut(s) 62
NlaIII CATG 4 cut(s) 71, 123, 245, 287
NlaIV GGNNCC 7 cut(s) 400, 401, 561, 596, 936, 985, 1106
NmuCI GTSAC 2 cut(s) 113, 1038
NspI RCATGY 2 cut(s) 123, 287
PceI AGGCCT 4 cut(s) 690, 699, 744, 762
PciI ACATGT 2 cut(s) 119, 283
PctI GAATGC 3 cut(s) 188, 474, 536
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 2 cut(s) 997, 1113
PfoI TCCNGGA 1 cut(s) 775
PkrI GCNGC 2 cut(s) 326, 802
PleI GAGTC 5 cut(s) 666, 702, 711, 720, 729
PpsI GAGTC 5 cut(s) 666, 702, 711, 720, 729
PpuMI RGGWCCY 2 cut(s) 399, 983
PscI ACATGT 2 cut(s) 119, 283
PshBI ATTAAT 1 cut(s) 941
Psp5II RGGWCCY 2 cut(s) 399, 983
PspFI CCCAGC 2 cut(s) 470, 1120
PspN4I GGNNCC 7 cut(s) 400, 401, 561, 596, 936, 985, 1106
PspPI GGNCC 3 cut(s) 399, 559, 983
PspPPI RGGWCCY 2 cut(s) 399, 983
PstI CTGCAG 1 cut(s) 91
PsuI RGATCY 2 cut(s) 594, 1104
SaqAI TTAA 3 cut(s) 216, 494, 941
SatI GCNGC 2 cut(s) 325, 801
Sau3AI GATC 4 cut(s) 79, 574, 594, 1104
Sau96I GGNCC 3 cut(s) 399, 559, 983
SchI GAGTC 5 cut(s) 666, 702, 711, 720, 729
SexAI ACCWGGT 1 cut(s) 1018
SfaNI GCATC 1 cut(s) 293
SfcI CTRYAG 1 cut(s) 87
SfiI GGCCNNNNNGGCC 1 cut(s) 696
SinI GGWCC 3 cut(s) 399, 559, 983
SmlI CTYRAG 2 cut(s) 380, 440
SmoI CTYRAG 2 cut(s) 380, 440
Sse9I AATT 9 cut(s) 248, 294, 481, 491, 496, 780, 903, 942, 949
SseBI AGGCCT 4 cut(s) 690, 699, 744, 762
SsiI CCGC 3 cut(s) 557, 987, 1043
SspMI CTAG 3 cut(s) 63, 591, 857
StuI AGGCCT 4 cut(s) 690, 699, 744, 762
StyI CCWWGG 2 cut(s) 241, 590
TaaI ACNGT 4 cut(s) 16, 106, 113, 341
TasI AATT 9 cut(s) 248, 294, 481, 491, 496, 780, 903, 942, 949
TfiI GAWTC 2 cut(s) 997, 1113
Tru1I TTAA 3 cut(s) 216, 494, 941
Tru9I TTAA 3 cut(s) 216, 494, 941
TscAI CASTG 4 cut(s) 109, 279, 334, 618
TseFI GTSAC 2 cut(s) 113, 1038
TseI GCWGC 2 cut(s) 324, 800
Tsp45I GTSAC 2 cut(s) 113, 1038
TspDTI ATGAA 3 cut(s) 35, 145, 804
TspRI CASTG 4 cut(s) 109, 279, 334, 618
VpaK11BI GGWCC 3 cut(s) 399, 559, 983
VspI ATTAAT 1 cut(s) 941
XagI CCTNNNNNAGG 1 cut(s) 963
XapI RAATTY 3 cut(s) 481, 496, 780
XceI RCATGY 2 cut(s) 123, 287
XmaJI CCTAGG 1 cut(s) 590
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 3 cut(s) 63, 591, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.