pycom09g00210

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001665
Physical Location & Seq
Forward (+)
196382 .. 197638
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00210.2

Sequence Viewer

Length: 786 bp
ATGTCTTGGTTTCTTACAGTTATCTTTCTGGTCACTACTCTTACATTTCATACTCCTTCAGAGGCTAGCTATGAGGAGATCCGGCCTTCTGCAGTAGTTGTTGGCACTGTCTACTGTGACACATGTTTCCAAGAGGATTTCTCAAATACCAGCCATTTCATTTCAGGGGCTTCTGTTGGTGTGGAATGCAAAGATGGGACTTCAACAAAACCAAGTTTCAACGCACAAGTGAAAACAAACAGCCATGGACAATTCAAAGTCCATCTGCCCTTCTCAGTGAGCAATGATGTCAAGAAAATTGAAGGATGCTCCGTGAAACTAATCCGCAGCAGTGAACCCTACTGTGCTGTGGCCTCATCAGCCACCTCATCTTCCCTACACCTCAAGGCAAGAAAGCAAGGGACCCACATATTCTCTGCTGGTTTCTTCATCTTCAAGCCTCTCAAGCAACCCAGCTTATGCAACCAGAAACCCAACATTCAGAATTCCAAGAAATTAAATTCCCAAAAGTTCTCAGAGATGGCATTCCCATCATCCTCTCAGGTGGACCCCAAAATGCCTGATCCCTCTCTCAACGAATCCGATCTCCTAGGTCTCCCAATCGGTCTACCAGGACTCCCAGGTCTCCCAGGACTCCCAGGCCTCCCAGGACTTCCAGGCCTGCCCCTTGCTGCTGAAAAAACTTCATCCAAGTCCTCCAACTCCAAAACTTCGAAGAATTGGCAACTAGGTGATAATAAAGAAGTAGCCCATCCAGATTCTTCACCAGCAGGTTCAGCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

262

Amino Acids

27.86

Weight (kDa)

8.68

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 761
AccI GTMKAC 2 cut(s) 111, 607
AciI CCGC 1 cut(s) 325
AclWI GGATC 2 cut(s) 73, 557
AcsI RAATTY 2 cut(s) 484, 499
AcuI CTGAAG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 5 cut(s) 204, 220, 256, 302, 436
AjnI CCWGG 6 cut(s) 610, 619, 628, 637, 646, 655
AluBI AGCT 2 cut(s) 69, 456
AluI AGCT 2 cut(s) 69, 456
Alw26I GTCTC 2 cut(s) 599, 629
AlwI GGATC 2 cut(s) 73, 557
AoxI GGCC 4 cut(s) 83, 351, 640, 658
ApeKI GCWGC 2 cut(s) 327, 671
ApoI RAATTY 2 cut(s) 484, 499
AspA2I CCTAGG 1 cut(s) 589
AspS9I GGNCC 2 cut(s) 402, 547
AsuHPI GGTGA 2 cut(s) 743, 756
AsuII TTCGAA 1 cut(s) 713
AsuNHI GCTAGC 1 cut(s) 65
AvaII GGWCC 2 cut(s) 402, 547
AvrII CCTAGG 1 cut(s) 589
BbvI GCAGC 2 cut(s) 339, 658
BccI CCATC 5 cut(s) 188, 270, 514, 538, 759
BciT130I CCWGG 6 cut(s) 612, 621, 630, 639, 648, 657
BcoDI GTCTC 2 cut(s) 599, 629
BfaI CTAG 3 cut(s) 66, 590, 728
BfmI CTRYAG 1 cut(s) 90
BfuAI ACCTGC 1 cut(s) 761
BisI GCNGC 2 cut(s) 328, 672
BlnI CCTAGG 1 cut(s) 589
BlsI GCNGC 2 cut(s) 329, 673
Bme1390I CCNGG 6 cut(s) 612, 621, 630, 639, 648, 657
Bme18I GGWCC 2 cut(s) 402, 547
BmgT120I GGNCC 2 cut(s) 402, 547
BmiI GGNNCC 3 cut(s) 403, 404, 549
BmrFI CCNGG 6 cut(s) 612, 621, 630, 639, 648, 657
BmsI GCATC 1 cut(s) 296
BmtI GCTAGC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 125, 157
Bpu14I TTCGAA 1 cut(s) 713
BpuEI CTTGAG 2 cut(s) 368, 428
BsaI GGTCTC 2 cut(s) 599, 629
BsaJI CCNNGG 6 cut(s) 244, 589, 619, 628, 637, 646
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 6 cut(s) 612, 621, 630, 639, 648, 657
BseDI CCNNGG 6 cut(s) 244, 589, 619, 628, 637, 646
BseGI GGATG 4 cut(s) 311, 533, 686, 751
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 3 cut(s) 288, 528, 554
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 2 cut(s) 339, 658
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 4 cut(s) 85, 353, 642, 660
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 2 cut(s) 211, 415
BsmAI GTCTC 2 cut(s) 599, 629
BsmFI GGGAC 2 cut(s) 211, 415
BsmI GAATGC 2 cut(s) 191, 524
BsnI GGCC 4 cut(s) 85, 353, 642, 660
Bso31I GGTCTC 2 cut(s) 599, 629
Bsp119I TTCGAA 1 cut(s) 713
Bsp143I GATC 3 cut(s) 78, 562, 583
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 325
BspANI GGCC 4 cut(s) 85, 353, 642, 660
BspCNI CTCAG 3 cut(s) 287, 527, 553
BspLI GGNNCC 3 cut(s) 403, 404, 549
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 761
BspOI GCTAGC 1 cut(s) 69
BspPI GGATC 2 cut(s) 73, 557
BspT104I TTCGAA 1 cut(s) 713
BspTNI GGTCTC 2 cut(s) 599, 629
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 6 cut(s) 244, 589, 619, 628, 637, 646
BssMI GATC 3 cut(s) 78, 562, 583
BssT1I CCWWGG 2 cut(s) 244, 589
Bst2UI CCWGG 6 cut(s) 612, 621, 630, 639, 648, 657
Bst4CI ACNGT 4 cut(s) 19, 109, 116, 344
BstBI TTCGAA 1 cut(s) 713
BstC8I GCNNGC 2 cut(s) 67, 662
BstDEI CTNAG 3 cut(s) 274, 514, 540
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 4 cut(s) 311, 533, 686, 751
BstKTI GATC 3 cut(s) 81, 565, 586
BstMAI GTCTC 2 cut(s) 599, 629
BstMBI GATC 3 cut(s) 78, 562, 583
BstMWI GCNNNNNNNGC 3 cut(s) 359, 445, 776
BstNI CCWGG 6 cut(s) 612, 621, 630, 639, 648, 657
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 6 cut(s) 610, 619, 628, 637, 646, 655
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 339, 658
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BsuRI GGCC 4 cut(s) 85, 353, 642, 660
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 4 cut(s) 311, 533, 686, 751
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 3 cut(s) 105, 282, 337
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 2 cut(s) 67, 662
Cfr13I GGNCC 2 cut(s) 402, 547
CviAII CATG 3 cut(s) 123, 245, 783
DdeI CTNAG 3 cut(s) 274, 514, 540
DpnI GATC 3 cut(s) 80, 564, 585
DpnII GATC 3 cut(s) 78, 562, 583
DrdI GACNNNNNNGTC 1 cut(s) 621
DseDI GACNNNNNNGTC 1 cut(s) 621
Eco130I CCWWGG 2 cut(s) 244, 589
Eco147I AGGCCT 2 cut(s) 642, 660
Eco31I GGTCTC 2 cut(s) 599, 629
Eco47I GGWCC 2 cut(s) 402, 547
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 484
EcoRII CCWGG 6 cut(s) 610, 619, 628, 637, 646, 655
EcoT14I CCWWGG 2 cut(s) 244, 589
ErhI CCWWGG 2 cut(s) 244, 589
FaeI CATG 3 cut(s) 126, 248, 786
FaiI YATR 7 cut(s) 51, 72, 124, 246, 410, 460, 784
FaqI GGGAC 2 cut(s) 211, 415
FatI CATG 3 cut(s) 122, 244, 782
FblI GTMKAC 2 cut(s) 111, 607
Fnu4HI GCNGC 2 cut(s) 328, 672
FokI GGATG 4 cut(s) 318, 520, 673, 738
Fsp4HI GCNGC 2 cut(s) 328, 672
FspBI CTAG 3 cut(s) 66, 590, 728
GluI GCNGC 2 cut(s) 328, 672
GsaI CCCAGC 1 cut(s) 456
HaeIII GGCC 4 cut(s) 85, 353, 642, 660
HapII CCGG 1 cut(s) 82
Hin1II CATG 3 cut(s) 126, 248, 786
HinfI GANTC 4 cut(s) 578, 615, 633, 758
HpaII CCGG 1 cut(s) 82
HphI GGTGA 2 cut(s) 743, 756
Hpy166II GTNNAC 4 cut(s) 112, 335, 547, 608
Hpy188I TCNGA 4 cut(s) 61, 483, 517, 583
Hpy188III TCNNGA 2 cut(s) 292, 755
Hpy8I GTNNAC 4 cut(s) 112, 335, 547, 608
HpyAV CCTTC 4 cut(s) 66, 96, 280, 296
HpyCH4III ACNGT 4 cut(s) 19, 109, 116, 344
HpyCH4V TGCA 3 cut(s) 92, 189, 462
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 445, 776
HpyF3I CTNAG 3 cut(s) 274, 514, 540
Hsp92II CATG 3 cut(s) 126, 248, 786
KflI GGGWCCC 1 cut(s) 402
Kzo9I GATC 3 cut(s) 78, 562, 583
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 2 cut(s) 339, 658
LweI GCATC 1 cut(s) 296
MaeI CTAG 3 cut(s) 66, 590, 728
MaeIII GTNAC 2 cut(s) 31, 116
MalI GATC 3 cut(s) 80, 564, 585
MboI GATC 3 cut(s) 78, 562, 583
MboII GAAGA 5 cut(s) 363, 418, 424, 727, 753
MflI RGATCY 1 cut(s) 78
MluCI AATT 6 cut(s) 251, 297, 484, 494, 499, 718
MlyI GAGTC 2 cut(s) 609, 627
MmeI TCCRAC 1 cut(s) 723
MseI TTAA 1 cut(s) 497
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 6 cut(s) 612, 621, 630, 639, 648, 657
Mva1269I GAATGC 2 cut(s) 191, 524
MvaI CCWGG 6 cut(s) 612, 621, 630, 639, 648, 657
MwoI GCNNNNNNNGC 3 cut(s) 359, 445, 776
NcoI CCATGG 1 cut(s) 244
NdeII GATC 3 cut(s) 78, 562, 583
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 3 cut(s) 126, 248, 786
NlaIV GGNNCC 3 cut(s) 403, 404, 549
NmuCI GTSAC 2 cut(s) 31, 116
NspI RCATGY 1 cut(s) 126
NspV TTCGAA 1 cut(s) 713
PceI AGGCCT 2 cut(s) 642, 660
PciI ACATGT 1 cut(s) 122
PctI GAATGC 2 cut(s) 191, 524
PfeI GAWTC 2 cut(s) 578, 758
PkrI GCNGC 2 cut(s) 329, 673
PleI GAGTC 2 cut(s) 609, 627
PpsI GAGTC 2 cut(s) 609, 627
PpuMI RGGWCCY 1 cut(s) 402
PscI ACATGT 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 6 cut(s) 610, 619, 628, 637, 646, 655
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 6 cut(s) 610, 619, 628, 637, 646, 655
PspN4I GGNNCC 3 cut(s) 403, 404, 549
PspPI GGNCC 2 cut(s) 402, 547
PspPPI RGGWCCY 1 cut(s) 402
PstI CTGCAG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 78
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 2 cut(s) 328, 672
Sau3AI GATC 3 cut(s) 78, 562, 583
Sau96I GGNCC 2 cut(s) 402, 547
SchI GAGTC 2 cut(s) 609, 627
ScrFI CCNGG 6 cut(s) 612, 621, 630, 639, 648, 657
SetI ASST 9 cut(s) 71, 368, 384, 458, 546, 595, 625, 733, 775
SfaNI GCATC 1 cut(s) 296
SfcI CTRYAG 1 cut(s) 90
SfuI TTCGAA 1 cut(s) 713
SinI GGWCC 2 cut(s) 402, 547
SmlI CTYRAG 2 cut(s) 383, 443
SmoI CTYRAG 2 cut(s) 383, 443
Sse9I AATT 6 cut(s) 251, 297, 484, 494, 499, 718
SseBI AGGCCT 2 cut(s) 642, 660
SsiI CCGC 1 cut(s) 325
SspMI CTAG 3 cut(s) 66, 590, 728
StuI AGGCCT 2 cut(s) 642, 660
StyD4I CCNGG 6 cut(s) 610, 619, 628, 637, 646, 655
StyI CCWWGG 2 cut(s) 244, 589
TaaI ACNGT 4 cut(s) 19, 109, 116, 344
TaqI TCGA 1 cut(s) 713
TaqII GACCGA 1 cut(s) 593
TasI AATT 6 cut(s) 251, 297, 484, 494, 499, 718
TfiI GAWTC 2 cut(s) 578, 758
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 3 cut(s) 112, 282, 337
TseFI GTSAC 2 cut(s) 31, 116
TseI GCWGC 2 cut(s) 327, 671
Tsp45I GTSAC 2 cut(s) 31, 116
TspDTI ATGAA 4 cut(s) 38, 148, 418, 675
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 3 cut(s) 112, 282, 337
VpaK11BI GGWCC 2 cut(s) 402, 547
XapI RAATTY 2 cut(s) 484, 499
XceI RCATGY 1 cut(s) 126
XmaJI CCTAGG 1 cut(s) 589
XmiI GTMKAC 2 cut(s) 111, 607
XspI CTAG 3 cut(s) 66, 590, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.