MD12G1250800.v1.1

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000953
Physical Location & Seq
Forward (+)
32080855 .. 32083055
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1250800.v1.1.491

Sequence Viewer

Length: 2109 bp
ATGGTGGATAGCCTGCTCCTTCTACCTGCGTTTTCCTTGCTAGTAATCGTCTTCTCCATGGCGATTAACGTCGTTCCAAGCGAAGCAGCAGCTTATGATCGTGTCGCTGACTGCCCGGTTTCAAACTGTAGCGACGCCGGCCCCCTCATCAAGTTTCCCTTCCGTCTCAAAGACCACCCCCTTCAATGTGGCCATCCAGAGTTTGAGGTTTCTTGCTTAAAAAACAAGACAATGATTCAGCTCTCCTCATCATCAGGACTGTTTCCCATCCAAAGTGTCAATTACCGCAGGCGAACTTTCAGCGTCTACGATGATGAAGAGGGTTGCTTTCCGAGACGCCTTCTTAATTTTACAATCTCCACCACCTCGCTCTTCGAACCAATACCTTCTCAGGAACCAATACCTTCTAGCGATCCCAGGATGGTCAATTCCGGGATGTACAGCTACCGTTTCAACTGTGCCGAATATAAAATTCATCCTAGCGATCTTCCTTACCCAGATGGGTACCCATATGACCCTTACCCATATGGGTACCCATATGACCCTTACCCATATGGCATGTATTATCCCCAGTTGGAGAAATTTACGCTCTTGAATTGTTCCACGACACAAACCTTGAACACGGGGAGCTCCTACATACCAATCACATGTCTTAGTGTCGGACATCATCAAGTTCTGGCTGTTTCGCCATCAGCTTCTGTCACGAAGCTGCCGTTCAGAACTTGCAGTACCTTGAAACAACTAGAGCTTCCGCTACGAAGAGTGGATGATGGCTATAGTACTGGGAATGACTGTGAGCCGGACAATGTTTTACTGCTGAAATGGAAATTAAATAGTTATTTCCCGCAATGTCAAAACTGTACAGAAGGAACAGGATCGTGTGAATTCAATGACACCAGCAACCGGATGGAGTGTCATCCTTACCCTATCGTTCGAGATCCCCCACAAGCGCTACCATTCCCAAAAGCGCCAAAACCAAAAGGTTGTCTATCAACTCGGAGGTCGATTATTGTGGGAGCAAGCTTAGCTTCTTTTGTTCTAATATCGGCAATGGCAGCAGTAGCAGTTTTCTTCTATGTAAAACAATCAAAGAGAAAACTGGAAAAGGAGAATCAACTGAAGGTTGAAAGGTTCCTAAAGGATCACAAATCTCACGTACCAACAAGATACTCCTATGCCAATATTAAAAAGATGACAAATGGATTCAAGAAGAAGTTAGGTGAAGGAAGTTTTGGAAGTGTTTACAGCGGAGAGCTTCCAACTAATGGAGTTCCAGTCGCCGTAAAAGTCCTCAGTGATTCGAAAGGAAACGGAGAAGATTTTGTTAATGAAGTGGGAACCATTGGCAGAATTCACCATGTCAATGTGGTTCGCCTACTTGGGTTTTCTGCTGAAGGAGGCAAGCGTGCGCTTATTTACGGGCTCATGCCAAACGGGTCCCTAGAGAACTTCATCACATATAAAGATCGATCCAACAACACTTCGTTTGATTGGCAGAAACTTCACAACATTGTCAATGGTACAGCGAAGGGAATCGAGTATCTTCACCAAGGGTGCGACCGCATGATCCTCCACTTTGATATCAAACCTCATAACATACTGTTAGACCATGATTTCAATCCTAAGATCTCGGATTTTGGTCTTGCTAAACTCTGTTCCAAGGAAGACAGTATCATATCTATGACAGCTGCTAGAGGCACCGTGGGCTACATTGCACCTGAAGTGTTCAATGGGAACTTCGGGAGCGTGTCCCACAAGTCAGATGTGTACAGTTTCGGTATGCTAGTGCTTGAAATTGTTGGATCCAGAAAACAACCTGCTCTTACATCTGGAACCAGTGAGGTCTACTTTCCGGAATTGGTTTACAATCGTCTAGTTCAAGGAGAAGCGTTGGATTTGAAGCTAGATACTGATGAAGATGCTCAGATTGCTAAGAAACTAGTGACTGTGGCATTTTGGTGCATCCAGTGGTACCCGGTGAATCGCCCTTCCATGAAAGCGGTTGTTAGAATGCTAGAAGGAGGCCTTGAAAACTTGATGATGCCACCAAATCCATTTGCTTCCACGAGTACTCAGACAGATCAACCAAGAACAACACTGTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

703

Amino Acids

78.27

Weight (kDa)

7.98

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 104 1.2e-14 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 402 - 672 2.3e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 403 - 670 1.8e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 34, 1825
Acc65I GGTACC 3 cut(s) 504, 531, 1971
AccB1I GGYRCC 4 cut(s) 504, 531, 1697, 1971
AccB7I CCANNNNNTGG 1 cut(s) 1265
AccI GTMKAC 2 cut(s) 306, 1845
AccIII TCCGGA 1 cut(s) 1852
AciI CCGC 6 cut(s) 286, 752, 845, 1248, 1561, 2000
AclWI GGATC 8 cut(s) 407, 883, 932, 1149, 1464, 1561, 1797, 1810
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 4 cut(s) 471, 581, 884, 1350
AcuI CTGAAG 3 cut(s) 1139, 1413, 1740
AcyI GRCGYC 2 cut(s) 135, 337
AfeI AGCGCT 1 cut(s) 951
AfiI CCNNNNNNNGG 2 cut(s) 903, 1265
AflIII ACRYGT 1 cut(s) 647
AhlI ACTAGT 1 cut(s) 1939
AjnI CCWGG 1 cut(s) 416
AjuI GAANNNNNNNTTGG 2 cut(s) 1215, 1247
Alw21I GWGCWC 1 cut(s) 632
Alw26I GTCTC 2 cut(s) 170, 328
AlwI GGATC 8 cut(s) 407, 883, 932, 1149, 1464, 1561, 1797, 1810
AlwNI CAGNNNCTG 1 cut(s) 698
Aor13HI TCCGGA 1 cut(s) 1852
Aor51HI AGCGCT 1 cut(s) 951
AoxI GGCC 3 cut(s) 139, 190, 2023
ApeKI GCWGC 5 cut(s) 86, 89, 709, 1055, 1688
ApoI RAATTY 4 cut(s) 471, 581, 884, 1350
Asp718I GGTACC 3 cut(s) 504, 531, 1971
AspLEI GCGC 3 cut(s) 952, 970, 1411
AspS9I GGNCC 2 cut(s) 140, 1437
AsuC2I CCSGG 3 cut(s) 116, 433, 1976
AsuHPI GGTGA 4 cut(s) 1232, 1346, 1538, 1990
AsuII TTCGAA 2 cut(s) 375, 1301
AvaII GGWCC 1 cut(s) 1437
BalI TGGCCA 1 cut(s) 192
BamHI GGATCC 1 cut(s) 1802
BanI GGYRCC 4 cut(s) 504, 531, 1697, 1971
BanII GRGCYC 2 cut(s) 632, 1425
BauI CACGAG 1 cut(s) 2065
BbsI GAAGAC 2 cut(s) 43, 1671
Bbv12I GWGCWC 1 cut(s) 632
BbvI GCAGC 5 cut(s) 98, 101, 696, 1067, 1675
BccI CCATC 7 cut(s) 201, 275, 415, 494, 697, 764, 901
BceAI ACGGC 2 cut(s) 697, 1265
BciT130I CCWGG 1 cut(s) 418
BcnI CCSGG 3 cut(s) 116, 433, 1976
BcoDI GTCTC 2 cut(s) 170, 328
BcuI ACTAGT 1 cut(s) 1939
BfmI CTRYAG 2 cut(s) 127, 775
BfoI RGCGCY 2 cut(s) 953, 971
BfuAI ACCTGC 2 cut(s) 34, 1825
BglII AGATCT 1 cut(s) 1626
BisI GCNGC 5 cut(s) 87, 90, 710, 1056, 1689
BlpI GCTNAGC 1 cut(s) 1024
BlsI GCNGC 5 cut(s) 88, 91, 711, 1057, 1690
BmcAI AGTACT 2 cut(s) 781, 2071
Bme1390I CCNGG 4 cut(s) 116, 418, 433, 1976
Bme18I GGWCC 1 cut(s) 1437
BmgT120I GGNCC 2 cut(s) 140, 1437
BmrFI CCNGG 4 cut(s) 116, 418, 433, 1976
BmrI ACTGGG 2 cut(s) 565, 792
BmsI GCATC 3 cut(s) 1909, 1971, 2031
BmuI ACTGGG 2 cut(s) 565, 792
BpiI GAAGAC 2 cut(s) 43, 1671
Bpu1102I GCTNAGC 1 cut(s) 1024
Bpu14I TTCGAA 2 cut(s) 375, 1301
BpuMI CCSGG 3 cut(s) 116, 433, 1976
Bsa29I ATCGAT 1 cut(s) 1468
BsaAI YACGTR 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 1617
BsaHI GRCGYC 2 cut(s) 135, 337
BsaJI CCNNGG 5 cut(s) 57, 416, 1549, 1659, 1701
BsaWI WCCGGW 2 cut(s) 903, 1852
BsaXI ACNNNNNCTCC 2 cut(s) 1260, 1290
Bsc4I CCNNNNNNNGG 2 cut(s) 903, 1265
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 6 cut(s) 571, 787, 1104, 1274, 1836, 1966
Bse3DI GCAATG 3 cut(s) 854, 1056, 1710
Bse8I GATNNNNATC 1 cut(s) 1617
BseAI TCCGGA 1 cut(s) 1852
BseBI CCWGG 1 cut(s) 418
BseCI ATCGAT 1 cut(s) 1468
BseDI CCNNGG 5 cut(s) 57, 416, 1549, 1659, 1701
BseGI GGATG 9 cut(s) 193, 267, 426, 441, 475, 772, 912, 916, 1962
BseJI GATNNNNATC 1 cut(s) 1617
BseLI CCNNNNNNNGG 2 cut(s) 903, 1265
BseMI GCAATG 3 cut(s) 854, 1056, 1710
BseMII CTCAG 4 cut(s) 404, 1306, 1937, 2087
BseNI ACTGG 6 cut(s) 571, 787, 1104, 1274, 1836, 1966
BseRI GAGGAG 1 cut(s) 235
BseXI GCAGC 5 cut(s) 98, 101, 696, 1067, 1675
Bsh1285I CGRYCG 1 cut(s) 1561
BshFI GGCC 3 cut(s) 141, 192, 2025
BshNI GGYRCC 4 cut(s) 504, 531, 1697, 1971
BshVI ATCGAT 1 cut(s) 1468
BsiEI CGRYCG 1 cut(s) 1561
BsiHKAI GWGCWC 1 cut(s) 632
BsiSI CCGG 7 cut(s) 116, 138, 432, 800, 904, 1853, 1976
BslFI GGGAC 2 cut(s) 1423, 1735
BslI CCNNNNNNNGG 2 cut(s) 903, 1265
BsmAI GTCTC 2 cut(s) 170, 328
BsmBI CGTCTC 2 cut(s) 170, 328
BsmFI GGGAC 2 cut(s) 1423, 1735
BsmI GAATGC 1 cut(s) 2016
BsnI GGCC 3 cut(s) 141, 192, 2025
Bsp119I TTCGAA 2 cut(s) 375, 1301
Bsp1286I GDGCHC 2 cut(s) 632, 1425
Bsp13I TCCGGA 1 cut(s) 1852
Bsp1407I TGTACA 3 cut(s) 438, 860, 1767
Bsp1720I GCTNAGC 1 cut(s) 1024
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 6 cut(s) 286, 752, 845, 1248, 1561, 2000
BspANI GGCC 3 cut(s) 141, 192, 2025
BspCNI CTCAG 4 cut(s) 403, 1305, 1936, 2086
BspDI ATCGAT 1 cut(s) 1468
BspEI TCCGGA 1 cut(s) 1852
BspMI ACCTGC 2 cut(s) 34, 1825
BspPI GGATC 8 cut(s) 407, 883, 932, 1149, 1464, 1561, 1797, 1810
BspQI GCTCTTC 1 cut(s) 377
BspT104I TTCGAA 2 cut(s) 375, 1301
BspT107I GGYRCC 4 cut(s) 504, 531, 1697, 1971
BsrDI GCAATG 3 cut(s) 854, 1056, 1710
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 3 cut(s) 438, 860, 1767
BsrI ACTGG 6 cut(s) 571, 787, 1104, 1274, 1836, 1966
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 5 cut(s) 57, 416, 1549, 1659, 1701
BssNI GRCGYC 2 cut(s) 135, 337
BssSI CACGAG 1 cut(s) 2065
BssT1I CCWWGG 3 cut(s) 57, 1549, 1659
Bst2BI CACGAG 1 cut(s) 2065
Bst2UI CCWGG 1 cut(s) 418
Bst6I CTCTTC 3 cut(s) 312, 377, 754
BstACI GRCGYC 2 cut(s) 135, 337
BstAUI TGTACA 3 cut(s) 438, 860, 1767
BstBAI YACGTR 1 cut(s) 1156
BstBI TTCGAA 2 cut(s) 375, 1301
BstC8I GCNNGC 6 cut(s) 14, 139, 290, 1021, 1403, 1407
BstDEI CTNAG 8 cut(s) 390, 653, 1024, 1292, 1623, 1923, 1932, 2073
BstDSI CCRYGG 2 cut(s) 57, 1701
BstF5I GGATG 9 cut(s) 193, 267, 426, 441, 475, 772, 912, 916, 1962
BstH2I RGCGCY 2 cut(s) 953, 971
BstHHI GCGC 3 cut(s) 952, 970, 1411
BstMAI GTCTC 2 cut(s) 170, 328
BstMCI CGRYCG 1 cut(s) 1561
BstMWI GCNNNNNNNGC 6 cut(s) 138, 1025, 1055, 1061, 1704, 1928
BstNI CCWGG 1 cut(s) 418
BstNSI RCATGY 2 cut(s) 562, 651
BstSCI CCNGG 4 cut(s) 114, 416, 431, 1974
BstSFI CTRYAG 2 cut(s) 127, 775
BstV1I GCAGC 5 cut(s) 98, 101, 696, 1067, 1675
BstV2I GAAGAC 2 cut(s) 43, 1671
BstX2I RGATCY 3 cut(s) 937, 1626, 1802
BstYI RGATCY 3 cut(s) 937, 1626, 1802
Bsu15I ATCGAT 1 cut(s) 1468
BsuRI GGCC 3 cut(s) 141, 192, 2025
BsuTUI ATCGAT 1 cut(s) 1468
BtgI CCRYGG 2 cut(s) 57, 1701
BtsCI GGATG 9 cut(s) 193, 267, 426, 441, 475, 772, 912, 916, 1962
BtsIMutI CAGTG 4 cut(s) 1300, 1843, 1973, 2096
BveI ACCTGC 2 cut(s) 34, 1825
Cac8I GCNNGC 6 cut(s) 14, 139, 290, 1021, 1403, 1407
CaiI CAGNNNCTG 1 cut(s) 698
CfoI GCGC 3 cut(s) 952, 970, 1411
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 140, 1437
ClaI ATCGAT 1 cut(s) 1468
CseI GACGC 3 cut(s) 143, 292, 345
CviAII CATG 8 cut(s) 58, 559, 648, 1358, 1426, 1564, 1610, 1993
DdeI CTNAG 8 cut(s) 390, 653, 1024, 1292, 1623, 1923, 1932, 2073
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 3 cut(s) 312, 377, 754
EarI CTCTTC 3 cut(s) 312, 377, 754
Ecl136II GAGCTC 1 cut(s) 630
Eco130I CCWWGG 3 cut(s) 57, 1549, 1659
Eco147I AGGCCT 1 cut(s) 2025
Eco24I GRGCYC 2 cut(s) 632, 1425
Eco32I GATATC 1 cut(s) 1582
Eco47I GGWCC 1 cut(s) 1437
Eco47III AGCGCT 1 cut(s) 951
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 3 cut(s) 1139, 1413, 1740
EcoICRI GAGCTC 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 1437
EcoRI GAATTC 2 cut(s) 884, 1350
EcoRII CCWGG 1 cut(s) 416
EcoRV GATATC 1 cut(s) 1582
EcoT14I CCWWGG 3 cut(s) 57, 1549, 1659
EcoT38I GRGCYC 2 cut(s) 632, 1425
ErhI CCWWGG 3 cut(s) 57, 1549, 1659
Esp3I CGTCTC 2 cut(s) 170, 328
FaeI CATG 8 cut(s) 61, 562, 651, 1361, 1429, 1567, 1613, 1996
FalI AAGNNNNNCTT 6 cut(s) 143, 175, 1012, 1044, 2010, 2042
FaqI GGGAC 2 cut(s) 1423, 1735
FatI CATG 8 cut(s) 57, 558, 647, 1357, 1425, 1563, 1609, 1992
FauI CCCGC 1 cut(s) 852
FauNDI CATATG 4 cut(s) 511, 526, 538, 553
FblI GTMKAC 2 cut(s) 306, 1845
Fnu4HI GCNGC 5 cut(s) 87, 90, 710, 1056, 1689
FokI GGATG 9 cut(s) 180, 254, 433, 448, 462, 779, 903, 919, 1949
FriOI GRGCYC 2 cut(s) 632, 1425
Fsp4HI GCNGC 5 cut(s) 87, 90, 710, 1056, 1689
GlaI GCGC 3 cut(s) 951, 969, 1410
GluI GCNGC 5 cut(s) 87, 90, 710, 1056, 1689
HaeII RGCGCY 2 cut(s) 953, 971
HaeIII GGCC 3 cut(s) 141, 192, 2025
HapII CCGG 7 cut(s) 116, 138, 432, 800, 904, 1853, 1976
HgaI GACGC 3 cut(s) 143, 292, 345
HhaI GCGC 3 cut(s) 952, 970, 1411
Hin1I GRCGYC 2 cut(s) 135, 337
Hin1II CATG 8 cut(s) 61, 562, 651, 1361, 1429, 1567, 1613, 1996
Hin6I GCGC 3 cut(s) 950, 968, 1409
HinP1I GCGC 3 cut(s) 950, 968, 1409
HindIII AAGCTT 1 cut(s) 1021
HinfI GANTC 6 cut(s) 235, 1111, 1203, 1298, 1533, 1981
HpaII CCGG 7 cut(s) 116, 138, 432, 800, 904, 1853, 1976
HphI GGTGA 4 cut(s) 1232, 1346, 1538, 1990
Hpy166II GTNNAC 5 cut(s) 307, 1243, 1768, 1846, 1864
Hpy188I TCNGA 8 cut(s) 333, 662, 719, 999, 1633, 1762, 1926, 2076
Hpy8I GTNNAC 5 cut(s) 307, 1243, 1768, 1846, 1864
Hpy99I CGWCG 2 cut(s) 74, 137
HpyCH4IV ACGT 2 cut(s) 69, 1155
HpyCH4V TGCA 3 cut(s) 726, 1715, 1962
HpyF10VI GCNNNNNNNGC 6 cut(s) 138, 1025, 1055, 1061, 1704, 1928
HpyF3I CTNAG 8 cut(s) 390, 653, 1024, 1292, 1623, 1923, 1932, 2073
HpySE526I ACGT 2 cut(s) 69, 1155
Hsp92I GRCGYC 2 cut(s) 135, 337
Hsp92II CATG 8 cut(s) 61, 562, 651, 1361, 1429, 1567, 1613, 1996
HspAI GCGC 3 cut(s) 950, 968, 1409
KflI GGGWCCC 1 cut(s) 1437
Kpn2I TCCGGA 1 cut(s) 1852
KpnI GGTACC 3 cut(s) 508, 535, 1975
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 5 cut(s) 21, 627, 635, 1016, 1743
Lsp1109I GCAGC 5 cut(s) 98, 101, 696, 1067, 1675
LweI GCATC 3 cut(s) 1909, 1971, 2031
MaeII ACGT 2 cut(s) 69, 1155
MaeIII GTNAC 2 cut(s) 700, 1942
MflI RGATCY 3 cut(s) 937, 1626, 1802
MhlI GDGCHC 2 cut(s) 632, 1425
MlsI TGGCCA 1 cut(s) 192
MluNI TGGCCA 1 cut(s) 192
MmeI TCCRAC 6 cut(s) 555, 640, 1283, 1497, 1780, 1872
Mox20I TGGCCA 1 cut(s) 192
MroI TCCGGA 1 cut(s) 1852
MroNI GCCGGC 1 cut(s) 137
MscI TGGCCA 1 cut(s) 192
MseI TTAA 7 cut(s) 66, 218, 345, 830, 1185, 1326, 2107
MslI CAYNNNNRTG 3 cut(s) 1362, 1679, 1957
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 2 cut(s) 1248, 1688
MspI CCGG 7 cut(s) 116, 138, 432, 800, 904, 1853, 1976
MspR9I CCNGG 4 cut(s) 116, 418, 433, 1976
Mva1269I GAATGC 1 cut(s) 2016
MvaI CCWGG 1 cut(s) 418
MwoI GCNNNNNNNGC 6 cut(s) 138, 1025, 1055, 1061, 1704, 1928
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 3 cut(s) 116, 433, 1976
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 4 cut(s) 511, 526, 538, 553
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 8 cut(s) 61, 562, 651, 1361, 1429, 1567, 1613, 1996
NmuCI GTSAC 2 cut(s) 700, 1942
NspI RCATGY 2 cut(s) 562, 651
NspV TTCGAA 2 cut(s) 375, 1301
PceI AGGCCT 1 cut(s) 2025
PciI ACATGT 1 cut(s) 647
PciSI GCTCTTC 1 cut(s) 377
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 710
PctI GAATGC 1 cut(s) 2016
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 6 cut(s) 235, 1111, 1203, 1298, 1533, 1981
PflMI CCANNNNNTGG 1 cut(s) 1265
PfoI TCCNGGA 1 cut(s) 431
PkrI GCNGC 5 cut(s) 88, 91, 711, 1057, 1690
Ppu21I YACGTR 1 cut(s) 1156
PpuMI RGGWCCY 1 cut(s) 1437
PscI ACATGT 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 1437
Psp6I CCWGG 1 cut(s) 416
PspGI CCWGG 1 cut(s) 416
PspPI GGNCC 2 cut(s) 140, 1437
PspPPI RGGWCCY 1 cut(s) 1437
PsrI GAACNNNNNNTAC 2 cut(s) 712, 744
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 3 cut(s) 937, 1626, 1802
PvuII CAGCTG 1 cut(s) 1688
RseI CAYNNNNRTG 3 cut(s) 1362, 1679, 1957
SacI GAGCTC 1 cut(s) 632
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 7 cut(s) 66, 218, 345, 830, 1185, 1326, 2107
SatI GCNGC 5 cut(s) 87, 90, 710, 1056, 1689
Sau96I GGNCC 2 cut(s) 140, 1437
ScaI AGTACT 2 cut(s) 781, 2071
ScrFI CCNGG 4 cut(s) 116, 418, 433, 1976
SduI GDGCHC 2 cut(s) 632, 1425
SfaNI GCATC 3 cut(s) 1909, 1971, 2031
SfcI CTRYAG 2 cut(s) 127, 775
SfuI TTCGAA 2 cut(s) 375, 1301
SinI GGWCC 1 cut(s) 1437
SmiMI CAYNNNNRTG 3 cut(s) 1362, 1679, 1957
SpeI ACTAGT 1 cut(s) 1939
SseBI AGGCCT 1 cut(s) 2025
SsiI CCGC 6 cut(s) 286, 752, 845, 1248, 1561, 2000
SspI AATATT 1 cut(s) 1183
SstI GAGCTC 1 cut(s) 632
StuI AGGCCT 1 cut(s) 2025
StyD4I CCNGG 4 cut(s) 114, 416, 431, 1974
StyI CCWWGG 3 cut(s) 57, 1549, 1659
TaiI ACGT 2 cut(s) 72, 1158
TaqI TCGA 6 cut(s) 375, 934, 1004, 1301, 1468, 1536
TatI WGTACW 5 cut(s) 438, 779, 860, 1767, 2069
TfiI GAWTC 6 cut(s) 235, 1111, 1203, 1298, 1533, 1981
Tru1I TTAA 7 cut(s) 66, 218, 345, 830, 1185, 1326, 2107
Tru9I TTAA 7 cut(s) 66, 218, 345, 830, 1185, 1326, 2107
TscAI CASTG 4 cut(s) 1300, 1843, 1973, 2103
TseFI GTSAC 2 cut(s) 700, 1942
TseI GCWGC 5 cut(s) 86, 89, 709, 1055, 1688
Tsp45I GTSAC 2 cut(s) 700, 1942
TspDTI ATGAA 6 cut(s) 330, 464, 1344, 1441, 1929, 2009
TspGWI ACGGA 2 cut(s) 152, 1326
TspRI CASTG 4 cut(s) 1300, 1843, 1973, 2103
Van91I CCANNNNNTGG 1 cut(s) 1265
VpaK11BI GGWCC 1 cut(s) 1437
XapI RAATTY 4 cut(s) 471, 581, 884, 1350
XceI RCATGY 2 cut(s) 562, 651
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XmiI GTMKAC 2 cut(s) 306, 1845
ZrmI AGTACT 2 cut(s) 781, 2071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.