Rmu_sc0003776.1_g000032

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003776.1
OG0000953
Physical Location & Seq
Reverse (-)
163281 .. 165340
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003776.1_g000032.1.cds

Sequence Viewer

Length: 1893 bp
atgggaattctgcagctgctgctacctttgtttttctgctccattgttcttatacccagcaaagcagctgatcatgactgtccagtttcaaagtgtagtcctgatggtccccttatcaagtttcccttccgtctaaagcatcaaccccttcactgtggcaatccagagtttgaggtttcctgcagcagcttcagcaatatgaccatgattcagctctcctcatcttcaggactcttcaccatccaaacaattgattatgaaaaccaatacttcgacgtctacaacagagatggctgccttcctagacggcttgtaaactttaccatctccagcagctcactctttcaaccagcacgtttatttgggaatgactactgcatcttttctgggaaatattttcccgacttaaaattactgaattgttccacaccagaaaactttagcacagggaatttcaacaagaagtacattcccatcacatgtcttagtataccagggcatcaggttgtggctactctaccctcagcttctttatctgacttgccggtgccaagatgccgtagtttgattggaccaatctatcttccactacgaagagtgggtgataatccggacaactgtgtaccagataatgtattaattctgaaatggaacttgagcgatttcccaggatgtcaaaactgtaaagcgggaaggtgccaattcaattataacagcaatcaattggagtgtcatcctgaactcagccctccacctccacctccatctccacctccaccttcacctggtgaatctaactcaaaaaggcgcaggtatatcataggagtgagcatagcttgttttgtgctcatctcggtagcatcggtagcaattttcttccatgtcacacaaacaaaaaggatagaggaaaaggagaatcaaatgaaaattgaaaggttcctaaatgatcacaagtctcatgtacccactagatactcctgtgctagtatacagaagatgacagatggctttaagaaaaagttaggtgaaggaggttttggaagtgttttcttgggaaagctacccaacggtgttcaagtcgccgtaaaagtcctcaatggttctaaaagaagtggagaagattttatcaatgaagtgggaaccattggtagaattcaccatgttaatgtggttcgcttacttgggttctctgctgaaggatgcaaccgtgctctcatttatgagttcatgccaaacaagtccttagaaaagttcatcacatctagagaatctaataataatgacactacatttgattggaagaaacttcagcacattatcacgggtataggaaaggggattgaataccttcaccaagggtgtgatcagaggatcctccactttgatatcaagcctcagaatatcttgctagaccatgacttcaaccctaagatctctgattttggtcttgcaaaactctgttccaaggatgaaagtataatatcaatgactgctgctagaggcacagtaggctacatagcacccgaagtgttcattaggaacttcacaaatgtgtcctacaaatcagatgtatatagttttgggatgctagtgcttgaaatgataggagctagaagggaacctcctcttggaactattaataacggtgaggtgtattttcctgaatggatttataagcgtctaattcagggaggagcattggatttgaagatagatactgatgaagatgctcatgttgcaaagaaactagtgattgttgcaatatggtgcatccagtggcacccggtgaatcgcccatccatgaaatctgttgttagaatgctagaaggagattctgaaagcttgatcatgccaccaaatccattcaccttcacaagtactcagacaagttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

630

Amino Acids

70.48

Weight (kDa)

8.53

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 711, 1674
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 801
AccB1I GGYRCC 3 cut(s) 547, 696, 1779
AccI GTMKAC 3 cut(s) 279, 490, 988
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 1 cut(s) 689
AclWI GGATC 2 cut(s) 1366, 1379
AcsI RAATTY 3 cut(s) 6, 451, 1152
AcuI CTGAAG 4 cut(s) 175, 210, 1215, 1292
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 2 cut(s) 788, 1786
AfaI GTAC 4 cut(s) 467, 624, 963, 1879
AfiI CCNNNNNNNGG 2 cut(s) 785, 1628
AflIII ACRYGT 1 cut(s) 479
AhlI ACTAGT 1 cut(s) 1747
AjnI CCWGG 3 cut(s) 493, 667, 784
AjuI GAANNNNNNNTTGG 4 cut(s) 1020, 1052, 1611, 1643
AleI CACNNNNGTG 1 cut(s) 1550
Alw21I GWGCWC 2 cut(s) 849, 1213
Alw26I GTCTC 1 cut(s) 960
AlwI GGATC 2 cut(s) 1366, 1379
AlwNI CAGNNNCTG 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 610
ApeKI GCWGC 9 cut(s) 13, 16, 19, 65, 183, 186, 294, 333, 1493
ApoI RAATTY 3 cut(s) 6, 451, 1152
ArsI GACNNNNNNTTYG 2 cut(s) 1274, 1306
AseI ATTAAT 2 cut(s) 638, 1638
Asp700I GAANNNNTTC 1 cut(s) 1347
AspLEI GCGC 1 cut(s) 810
AspS9I GGNCC 2 cut(s) 107, 572
AsuC2I CCSGG 1 cut(s) 1784
AvaII GGWCC 2 cut(s) 107, 572
BaeI ACNNNNGTAYC 2 cut(s) 964, 997
BamHI GGATCC 1 cut(s) 1371
BanI GGYRCC 3 cut(s) 547, 696, 1779
Bbv12I GWGCWC 2 cut(s) 849, 1213
BbvCI CCTCAGC 1 cut(s) 523
BbvI GCAGC 9 cut(s) 3, 6, 25, 77, 195, 198, 281, 345, 1480
BccI CCATC 8 cut(s) 98, 248, 284, 332, 482, 772, 998, 1804
BceAI ACGGC 3 cut(s) 323, 543, 1067
BciT130I CCWGG 3 cut(s) 495, 669, 786
BclI TGATCA 4 cut(s) 70, 946, 1363, 1845
BcnI CCSGG 1 cut(s) 1784
BcoDI GTCTC 1 cut(s) 960
BcuI ACTAGT 1 cut(s) 1747
BfmI CTRYAG 2 cut(s) 11, 181
BfuAI ACCTGC 1 cut(s) 801
BglII AGATCT 1 cut(s) 1431
BisI GCNGC 9 cut(s) 14, 17, 20, 66, 184, 187, 295, 334, 1494
BlsI GCNGC 9 cut(s) 15, 18, 21, 67, 185, 188, 296, 335, 1495
BmcAI AGTACT 1 cut(s) 1879
Bme1390I CCNGG 4 cut(s) 495, 669, 786, 1784
Bme18I GGWCC 2 cut(s) 107, 572
BmgT120I GGNCC 2 cut(s) 107, 572
BmiI GGNNCC 8 cut(s) 109, 549, 698, 938, 1141, 1373, 1620, 1781
BmrFI CCNGG 4 cut(s) 495, 669, 786, 1784
BmsI GCATC 9 cut(s) 148, 387, 508, 545, 869, 1190, 1575, 1717, 1779
BpmI CTGGAG 1 cut(s) 313
Bpu10I CCTNAGC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 676
BpuMI CCSGG 1 cut(s) 1784
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 494, 667, 1354, 1464
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 2 cut(s) 785, 1628
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 2 cut(s) 83, 1774
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 3 cut(s) 495, 669, 786
BseDI CCNNGG 4 cut(s) 494, 667, 1354, 1464
BseGI GGATG 8 cut(s) 240, 677, 733, 1205, 1474, 1590, 1770, 1796
BseLI CCNNNNNNNGG 2 cut(s) 785, 1628
BseMII CTCAG 3 cut(s) 537, 757, 1409
BseNI ACTGG 2 cut(s) 83, 1774
BseRI GAGGAG 3 cut(s) 208, 1614, 1707
BseXI GCAGC 9 cut(s) 3, 6, 25, 77, 195, 198, 281, 345, 1480
BseYI CCCAGC 1 cut(s) 56
BshNI GGYRCC 3 cut(s) 547, 696, 1779
BsiHKAI GWGCWC 2 cut(s) 849, 1213
BsiSI CCGG 3 cut(s) 545, 611, 1784
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 785, 1628
BsmAI GTCTC 1 cut(s) 960
BsmFI GGGAC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 1824
Bsp1286I GDGCHC 2 cut(s) 849, 1213
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
BspACI CCGC 1 cut(s) 689
BspCNI CTCAG 3 cut(s) 536, 756, 1408
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 8 cut(s) 109, 549, 698, 938, 1141, 1373, 1620, 1781
BspMAI CTGCAG 2 cut(s) 15, 185
BspMI ACCTGC 1 cut(s) 801
BspPI GGATC 2 cut(s) 1366, 1379
BspT107I GGYRCC 3 cut(s) 547, 696, 1779
BsrFI RCCGGY 1 cut(s) 544
BsrI ACTGG 2 cut(s) 83, 1774
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 4 cut(s) 494, 667, 1354, 1464
BssMI GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
BssNAI GTATAC 2 cut(s) 491, 989
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 1354, 1464
Bst1107I GTATAC 2 cut(s) 491, 989
Bst2UI CCWGG 3 cut(s) 495, 669, 786
Bst4CI ACNGT 8 cut(s) 80, 155, 620, 683, 1070, 1208, 1507, 1646
Bst6I CTCTTC 2 cut(s) 239, 589
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 19
BstDEI CTNAG 7 cut(s) 485, 523, 743, 1243, 1395, 1428, 1881
BstF5I GGATG 8 cut(s) 240, 677, 733, 1205, 1474, 1590, 1770, 1796
BstHHI GCGC 1 cut(s) 810
BstKTI GATC 6 cut(s) 73, 949, 1366, 1374, 1434, 1848
BstMAI GTCTC 1 cut(s) 960
BstMBI GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
BstMWI GCNNNNNNNGC 5 cut(s) 19, 192, 866, 1509, 1736
BstNI CCWGG 3 cut(s) 495, 669, 786
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 4 cut(s) 493, 667, 784, 1782
BstSFI CTRYAG 2 cut(s) 11, 181
BstV1I GCAGC 9 cut(s) 3, 6, 25, 77, 195, 198, 281, 345, 1480
BstX2I RGATCY 2 cut(s) 1371, 1431
BstYI RGATCY 2 cut(s) 1371, 1431
BstZ17I GTATAC 2 cut(s) 491, 989
BtsCI GGATG 8 cut(s) 240, 677, 733, 1205, 1474, 1590, 1770, 1796
BtsIMutI CAGTG 2 cut(s) 151, 1781
BveI ACCTGC 1 cut(s) 801
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 810
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 2 cut(s) 107, 572
CseI GACGC 1 cut(s) 1667
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 4 cut(s) 466, 623, 962, 1878
CviQI GTAC 4 cut(s) 466, 623, 962, 1878
DdeI CTNAG 7 cut(s) 485, 523, 743, 1243, 1395, 1428, 1881
DpnI GATC 6 cut(s) 72, 948, 1365, 1373, 1433, 1847
DpnII GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
DraIII CACNNNGTG 2 cut(s) 788, 1786
Eam1104I CTCTTC 2 cut(s) 239, 589
EarI CTCTTC 2 cut(s) 239, 589
Eco130I CCWWGG 2 cut(s) 1354, 1464
Eco32I GATATC 1 cut(s) 1387
Eco47I GGWCC 2 cut(s) 107, 572
Eco57I CTGAAG 4 cut(s) 175, 210, 1215, 1292
EcoRI GAATTC 2 cut(s) 6, 1152
EcoRII CCWGG 3 cut(s) 493, 667, 784
EcoRV GATATC 1 cut(s) 1387
EcoT14I CCWWGG 2 cut(s) 1354, 1464
ErhI CCWWGG 2 cut(s) 1354, 1464
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 93
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 4 cut(s) 70, 946, 1363, 1845
FblI GTMKAC 3 cut(s) 279, 490, 988
Fnu4HI GCNGC 9 cut(s) 14, 17, 20, 66, 184, 187, 295, 334, 1494
FokI GGATG 8 cut(s) 227, 684, 720, 1212, 1481, 1597, 1757, 1783
Fsp4HI GCNGC 9 cut(s) 14, 17, 20, 66, 184, 187, 295, 334, 1494
GlaI GCGC 1 cut(s) 809
GluI GCNGC 9 cut(s) 14, 17, 20, 66, 184, 187, 295, 334, 1494
GsaI CCCAGC 1 cut(s) 60
GsuI CTGGAG 1 cut(s) 313
HapII CCGG 3 cut(s) 545, 611, 1784
HgaI GACGC 1 cut(s) 1667
HhaI GCGC 1 cut(s) 810
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 1 cut(s) 808
HinP1I GCGC 1 cut(s) 808
HindIII AAGCTT 1 cut(s) 1840
HinfI GANTC 7 cut(s) 208, 231, 791, 916, 1268, 1789, 1832
HpaII CCGG 3 cut(s) 545, 611, 1784
Hpy166II GTNNAC 5 cut(s) 280, 316, 491, 623, 989
Hpy188I TCNGA 8 cut(s) 538, 645, 1368, 1398, 1438, 1567, 1837, 1884
Hpy188III TCNNGA 9 cut(s) 74, 101, 164, 228, 401, 611, 737, 1263, 1661
Hpy8I GTNNAC 5 cut(s) 280, 316, 491, 623, 989
Hpy99I CGWCG 1 cut(s) 278
HpyCH4III ACNGT 8 cut(s) 80, 155, 620, 683, 1070, 1208, 1507, 1646
HpyCH4IV ACGT 2 cut(s) 276, 355
HpyCH4V TGCA 8 cut(s) 13, 183, 378, 1203, 1451, 1739, 1760, 1770
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 192, 866, 1509, 1736
HpyF3I CTNAG 7 cut(s) 485, 523, 743, 1243, 1395, 1428, 1881
HpySE526I ACGT 2 cut(s) 276, 355
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 1 cut(s) 808
Kpn2I TCCGGA 1 cut(s) 610
Ksp22I TGATCA 4 cut(s) 70, 946, 1363, 1845
Kzo9I GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
LmnI GCTCC 3 cut(s) 44, 1607, 1694
Lsp1109I GCAGC 9 cut(s) 3, 6, 25, 77, 195, 198, 281, 345, 1480
LweI GCATC 9 cut(s) 148, 387, 508, 545, 869, 1190, 1575, 1717, 1779
MabI ACCWGGT 1 cut(s) 784
MaeII ACGT 2 cut(s) 276, 355
MaeIII GTNAC 1 cut(s) 883
MalI GATC 6 cut(s) 72, 948, 1365, 1373, 1433, 1847
MboI GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
MfeI CAATTG 2 cut(s) 249, 722
MflI RGATCY 2 cut(s) 1371, 1431
MhlI GDGCHC 2 cut(s) 849, 1213
MlyI GAGTC 1 cut(s) 225
MroI TCCGGA 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 1347
MseI TTAA 5 cut(s) 407, 638, 1011, 1164, 1638
MslI CAYNNNNRTG 3 cut(s) 824, 1164, 1550
MspA1I CMGCKG 2 cut(s) 16, 68
MspI CCGG 3 cut(s) 545, 611, 1784
MspR9I CCNGG 4 cut(s) 495, 669, 786, 1784
MunI CAATTG 2 cut(s) 249, 722
Mva1269I GAATGC 1 cut(s) 1824
MvaI CCWGG 3 cut(s) 495, 669, 786
MwoI GCNNNNNNNGC 5 cut(s) 19, 192, 866, 1509, 1736
NciI CCSGG 1 cut(s) 1784
NdeII GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
NlaIV GGNNCC 8 cut(s) 109, 549, 698, 938, 1141, 1373, 1620, 1781
NmuCI GTSAC 1 cut(s) 883
NspI RCATGY 1 cut(s) 483
OliI CACNNNNGTG 1 cut(s) 1550
PagI TCATGA 1 cut(s) 73
PciI ACATGT 1 cut(s) 479
PctI GAATGC 1 cut(s) 1824
PdmI GAANNNNTTC 1 cut(s) 1347
PfeI GAWTC 6 cut(s) 208, 791, 916, 1268, 1789, 1832
PkrI GCNGC 9 cut(s) 15, 18, 21, 67, 185, 188, 296, 335, 1495
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PscI ACATGT 1 cut(s) 479
PshBI ATTAAT 2 cut(s) 638, 1638
PsiI TTATAA 2 cut(s) 711, 1674
Psp6I CCWGG 3 cut(s) 493, 667, 784
PspFI CCCAGC 1 cut(s) 56
PspGI CCWGG 3 cut(s) 493, 667, 784
PspN4I GGNNCC 8 cut(s) 109, 549, 698, 938, 1141, 1373, 1620, 1781
PspPI GGNCC 2 cut(s) 107, 572
PstI CTGCAG 2 cut(s) 15, 185
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 2 cut(s) 1371, 1431
PvuII CAGCTG 2 cut(s) 16, 68
RsaI GTAC 4 cut(s) 467, 624, 963, 1879
RsaNI GTAC 4 cut(s) 466, 623, 962, 1878
RseI CAYNNNNRTG 3 cut(s) 824, 1164, 1550
SaqAI TTAA 5 cut(s) 407, 638, 1011, 1164, 1638
SatI GCNGC 9 cut(s) 14, 17, 20, 66, 184, 187, 295, 334, 1494
Sau3AI GATC 6 cut(s) 70, 946, 1363, 1371, 1431, 1845
Sau96I GGNCC 2 cut(s) 107, 572
ScaI AGTACT 1 cut(s) 1879
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 4 cut(s) 495, 669, 786, 1784
SduI GDGCHC 2 cut(s) 849, 1213
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 9 cut(s) 148, 387, 508, 545, 869, 1190, 1575, 1717, 1779
SfcI CTRYAG 2 cut(s) 11, 181
SinI GGWCC 2 cut(s) 107, 572
SmiMI CAYNNNNRTG 3 cut(s) 824, 1164, 1550
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
SpeI ACTAGT 1 cut(s) 1747
SsiI CCGC 1 cut(s) 689
SspI AATATT 1 cut(s) 395
StyD4I CCNGG 4 cut(s) 493, 667, 784, 1782
StyI CCWWGG 2 cut(s) 1354, 1464
TaaI ACNGT 8 cut(s) 80, 155, 620, 683, 1070, 1208, 1507, 1646
TaiI ACGT 2 cut(s) 279, 358
TaqI TCGA 1 cut(s) 273
TatI WGTACW 2 cut(s) 465, 1877
TfiI GAWTC 6 cut(s) 208, 791, 916, 1268, 1789, 1832
Tru1I TTAA 5 cut(s) 407, 638, 1011, 1164, 1638
Tru9I TTAA 5 cut(s) 407, 638, 1011, 1164, 1638
TscAI CASTG 2 cut(s) 158, 1781
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 9 cut(s) 13, 16, 19, 65, 183, 186, 294, 333, 1493
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 9 cut(s) 273, 938, 1146, 1216, 1243, 1485, 1522, 1737, 1817
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 2 cut(s) 158, 1781
VpaK11BI GGWCC 2 cut(s) 107, 572
VspI ATTAAT 2 cut(s) 638, 1638
XapI RAATTY 3 cut(s) 6, 451, 1152
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 1 cut(s) 483
XmiI GTMKAC 3 cut(s) 279, 490, 988
XmnI GAANNNNTTC 1 cut(s) 1347
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 1879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.