Rh7BG245800

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000953
Physical Location & Seq
Reverse (-)
21900348 .. 21902598
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG245800.1

Sequence Viewer

Length: 1674 bp
ATGATTCAGCTCTCCTCATCTTCAGGACTCTTCACCATCCAAACAATTGATTATGAAAACCAATACTTCGACGTCTACAACAGAGATGGCTGCCTTCCTAGACGGCTTGTAAACTTTACCATCTCCAGCAGCTCACTCTTTCAACCAGCACGTTTATTTGGGAATGACTACTGCATCTTTTCTGGGAAATATTTTCCCGACTTAAAATTACTGAATTGTTCCACACCAGAAAACTTTAGCACGGGGAATTTCAACAAGAAGTACATTCCCATCACATGTCTTAGTATACCAGGGTATCAGGTTGTGGCTACTCTACCCTCAACTTCTTTCCTTGACTTGCCGGTGCCAACTTGCCGTAGTTTGATTGGACCAATCTATCTTCCACTACGAAGAGTGGATGGTAATCCGGACAACTGTGTACCGGATAGTGTATTAATTCTGAAATGGGACTTGGGGAATTTCACAGGATGTCAAAACTGTAAAGCGGGAAGGTGCCAATTCAATTATAACAGCAATCAATTGGAGTGTCATCCTGAACTCAGCCCTCCATCTCCACCTCCACCTTCACCTGGTGTATCTAACTCAAAAAGGCCCAGGTATATAATAGGAGTGAGCATAGCTTGTTTTGTTCTCATCTCAGTAGCATCGGTAGCAATTTTCTTCCATGTCACACAAACAAAAAGGATAGAGGAAAAGGAGAATCAAATGAAAATTGAAAGGTTCCTAAATGATCACAAGTCTCATGTACCCACTAGATACTCCTGTGCTAGTATAAAGAAGATGACAGTTGGCTTTAAGAAAAAGTTAGGTGAAGGAGGTTTTGGAAGTGTTTTCTTGGGAAAGCTACCCAACGGTGTTCAAGTCGCCGTAAAAGTCCTCAATGGTTCTAAAAGAAGTGGAGAAGATTTTATCAACGAAGTGGGAACCATTGGTAGAATTCACCATGTTAATGTGGTTCGCTTACTTGGGTTCTCTGCTGAAGGATGCAAGCGTGCACTCATTTATGAGTTCATGCCAAACAAGTCCTTAGAAAAGTTCATCACATCTAGAGAATCTAATAATAATGACACTACATTTGATTGGAAGAAACTTCAGCACATTATCACGGGTATAGGAAAGGGGATTGAATACCTTCACCAAGGGTGTGATCAGAGGATCCTCCACTTTGATATCAAGCCTCATAATATCTTGTTAGACCATGACTTCAACCCTAAGATCTCTGATTTTGGTCTTGCAAAACTCTGTTCCAAGGAAGAAAGTATTATATCAATGACTGCTGCTAGAGGCACAGTCGGCTACATCGCACCCGAAGTGTTCATTAGGAACTTCGCAAACGTGTCCTACAAATCAGATGTATATAGTTTTGGGATGCTAGTGCTTGAAATGATAGGAGCTAGAAGGGAACCTGCTCTTGGAACTATTAACAGTGAGGTGTATTTTCCTGAATGGATTTATAAGCGTCTAATTCAGGGAGGAACATTGGATTTGAAGCTAGATACTGATGAAGATGCTCATGTTGCAAAGAAACTAGTGATTGTTGCACTATGGTGCATTCAGTGGCACCCGGTGAATCGCCCATCCATGAAATCTGTTGTTAGAATGCTGGAAGGAGATTCTGAAAGCTTGATCATGCCACCAAATCCATTCACCTTCACAAGTACTCAGACAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

557

Amino Acids

62.43

Weight (kDa)

8.78

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 265 - 535 1.7e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 266 - 533 1e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 507, 1455
AatII GACGTC 1 cut(s) 75
Acc36I ACCTGC 1 cut(s) 1414
AccB1I GGYRCC 3 cut(s) 343, 492, 1560
AccI GTMKAC 2 cut(s) 75, 286
AccIII TCCGGA 1 cut(s) 406
AciI CCGC 1 cut(s) 485
AclWI GGATC 2 cut(s) 1150, 1163
AcsI RAATTY 3 cut(s) 247, 457, 936
AcuI CTGAAG 3 cut(s) 6, 999, 1076
AcyI GRCGYC 1 cut(s) 72
AdeI CACNNNGTG 2 cut(s) 572, 1567
AfaI GTAC 4 cut(s) 263, 420, 747, 1660
AfiI CCNNNNNNNGG 2 cut(s) 569, 1412
AflIII ACRYGT 2 cut(s) 275, 1335
AgsI TTSAA 9 cut(s) 143, 253, 502, 716, 860, 1127, 1207, 1382, 1489
AhlI ACTAGT 1 cut(s) 1528
AjnI CCWGG 3 cut(s) 289, 568, 593
AjuI GAANNNNNNNTTGG 6 cut(s) 434, 466, 804, 836, 1395, 1427
AleI CACNNNNGTG 1 cut(s) 1546
AluBI AGCT 7 cut(s) 10, 132, 620, 844, 1394, 1492, 1623
AluI AGCT 7 cut(s) 10, 132, 620, 844, 1394, 1492, 1623
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 1 cut(s) 744
Alw44I GTGCAC 1 cut(s) 993
AlwI GGATC 2 cut(s) 1150, 1163
Aor13HI TCCGGA 1 cut(s) 406
AoxI GGCC 1 cut(s) 590
ApaLI GTGCAC 1 cut(s) 993
ApeKI GCWGC 3 cut(s) 90, 129, 1277
ApoI RAATTY 3 cut(s) 247, 457, 936
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
AseI ATTAAT 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 1131
AspS9I GGNCC 2 cut(s) 368, 591
AsuC2I CCSGG 1 cut(s) 1565
AsuHPI GGTGA 7 cut(s) 25, 558, 821, 932, 1127, 1579, 1639
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 997
BaeI ACNNNNGTAYC 4 cut(s) 278, 311, 748, 781
BamHI GGATCC 1 cut(s) 1155
BanI GGYRCC 3 cut(s) 343, 492, 1560
Bbv12I GWGCWC 1 cut(s) 997
BbvI GCAGC 3 cut(s) 77, 141, 1264
BccI CCATC 7 cut(s) 44, 80, 128, 278, 392, 556, 1585
BceAI ACGGC 3 cut(s) 119, 339, 851
BciT130I CCWGG 3 cut(s) 291, 570, 595
BclI TGATCA 3 cut(s) 730, 1147, 1626
BcnI CCSGG 1 cut(s) 1565
BcoDI GTCTC 1 cut(s) 744
BcuI ACTAGT 1 cut(s) 1528
BfaI CTAG 9 cut(s) 99, 753, 768, 1047, 1281, 1373, 1395, 1493, 1529
BfuAI ACCTGC 1 cut(s) 1414
BglII AGATCT 1 cut(s) 1215
BisI GCNGC 3 cut(s) 91, 130, 1278
BlsI GCNGC 3 cut(s) 92, 131, 1279
BmcAI AGTACT 1 cut(s) 1660
Bme1390I CCNGG 4 cut(s) 291, 570, 595, 1565
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 368, 591
BmiI GGNNCC 7 cut(s) 345, 494, 722, 925, 1157, 1404, 1562
BmrFI CCNGG 4 cut(s) 291, 570, 595, 1565
BmsI GCATC 5 cut(s) 183, 653, 974, 1359, 1498
BpmI CTGGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 1565
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 290, 593, 1138, 1248
BsaWI WCCGGW 2 cut(s) 406, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 569, 1412
Bse118I RCCGGY 1 cut(s) 340
Bse8I GATNNNNATC 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 406
BseBI CCWGG 3 cut(s) 291, 570, 595
BseDI CCNNGG 4 cut(s) 290, 593, 1138, 1248
BseGI GGATG 7 cut(s) 36, 403, 473, 529, 989, 1374, 1577
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 2 cut(s) 569, 1412
BseMII CTCAG 2 cut(s) 553, 651
BseRI GAGGAG 1 cut(s) 4
BseSI GKGCMC 1 cut(s) 997
BseXI GCAGC 3 cut(s) 77, 141, 1264
BshFI GGCC 1 cut(s) 592
BshNI GGYRCC 3 cut(s) 343, 492, 1560
BsiHKAI GWGCWC 1 cut(s) 997
BsiSI CCGG 4 cut(s) 341, 407, 422, 1565
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 2 cut(s) 569, 1412
BsmAI GTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 461
BsmI GAATGC 2 cut(s) 1551, 1605
BsnI GGCC 1 cut(s) 592
Bsp1286I GDGCHC 1 cut(s) 997
Bsp13I TCCGGA 1 cut(s) 406
Bsp143I GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
BspACI CCGC 1 cut(s) 485
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 2 cut(s) 552, 650
BspEI TCCGGA 1 cut(s) 406
BspLI GGNNCC 7 cut(s) 345, 494, 722, 925, 1157, 1404, 1562
BspMI ACCTGC 1 cut(s) 1414
BspPI GGATC 2 cut(s) 1150, 1163
BspT107I GGYRCC 3 cut(s) 343, 492, 1560
BsrFI RCCGGY 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 4 cut(s) 290, 593, 1138, 1248
BssMI GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
BssNAI GTATAC 1 cut(s) 287
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 1138, 1248
Bst1107I GTATAC 1 cut(s) 287
Bst2UI CCWGG 3 cut(s) 291, 570, 595
Bst4CI ACNGT 6 cut(s) 416, 479, 787, 854, 1291, 1427
Bst6I CTCTTC 2 cut(s) 35, 385
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 2 cut(s) 989, 993
BstDEI CTNAG 6 cut(s) 281, 539, 637, 1027, 1212, 1662
BstF5I GGATG 7 cut(s) 36, 403, 473, 529, 989, 1374, 1577
BstKTI GATC 5 cut(s) 733, 1150, 1158, 1218, 1629
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
BstMWI GCNNNNNNNGC 3 cut(s) 650, 1293, 1517
BstNI CCWGG 3 cut(s) 291, 570, 595
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 4 cut(s) 289, 568, 593, 1563
BstSLI GKGCMC 1 cut(s) 997
BstV1I GCAGC 3 cut(s) 77, 141, 1264
BstX2I RGATCY 2 cut(s) 1155, 1215
BstYI RGATCY 2 cut(s) 1155, 1215
BstZ17I GTATAC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 592
BtgZI GCGATG 1 cut(s) 1285
BtsCI GGATG 7 cut(s) 36, 403, 473, 529, 989, 1374, 1577
BtsIMutI CAGTG 2 cut(s) 1432, 1562
BveI ACCTGC 1 cut(s) 1414
Cac8I GCNNGC 2 cut(s) 989, 993
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 368, 591
CseI GACGC 1 cut(s) 1448
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 4 cut(s) 262, 419, 746, 1659
CviAII CATG 9 cut(s) 276, 665, 743, 944, 1012, 1199, 1514, 1582, 1630
CviQI GTAC 4 cut(s) 262, 419, 746, 1659
DdeI CTNAG 6 cut(s) 281, 539, 637, 1027, 1212, 1662
DpnI GATC 5 cut(s) 732, 1149, 1157, 1217, 1628
DpnII GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
DraIII CACNNNGTG 2 cut(s) 572, 1567
Eam1104I CTCTTC 2 cut(s) 35, 385
EarI CTCTTC 2 cut(s) 35, 385
Eco130I CCWWGG 2 cut(s) 1138, 1248
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 3 cut(s) 6, 999, 1076
EcoRI GAATTC 1 cut(s) 936
EcoRII CCWGG 3 cut(s) 289, 568, 593
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 2 cut(s) 1138, 1248
ErhI CCWWGG 2 cut(s) 1138, 1248
FaeI CATG 9 cut(s) 279, 668, 746, 947, 1015, 1202, 1517, 1585, 1633
FaqI GGGAC 1 cut(s) 461
FatI CATG 9 cut(s) 275, 664, 742, 943, 1011, 1198, 1513, 1581, 1629
FauI CCCGC 1 cut(s) 478
FbaI TGATCA 3 cut(s) 730, 1147, 1626
FblI GTMKAC 2 cut(s) 75, 286
Fnu4HI GCNGC 3 cut(s) 91, 130, 1278
FokI GGATG 7 cut(s) 23, 410, 480, 516, 996, 1381, 1564
Fsp4HI GCNGC 3 cut(s) 91, 130, 1278
FspBI CTAG 9 cut(s) 99, 753, 768, 1047, 1281, 1373, 1395, 1493, 1529
GluI GCNGC 3 cut(s) 91, 130, 1278
GsuI CTGGAG 1 cut(s) 109
HaeIII GGCC 1 cut(s) 592
HapII CCGG 4 cut(s) 341, 407, 422, 1565
HgaI GACGC 1 cut(s) 1448
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 9 cut(s) 279, 668, 746, 947, 1015, 1202, 1517, 1585, 1633
HindIII AAGCTT 1 cut(s) 1621
HinfI GANTC 6 cut(s) 4, 27, 700, 1052, 1570, 1613
HpaII CCGG 4 cut(s) 341, 407, 422, 1565
HphI GGTGA 7 cut(s) 25, 558, 821, 932, 1127, 1579, 1639
Hpy166II GTNNAC 5 cut(s) 76, 112, 287, 419, 995
Hpy188I TCNGA 6 cut(s) 441, 1152, 1222, 1351, 1618, 1665
Hpy188III TCNNGA 6 cut(s) 24, 197, 407, 533, 1047, 1442
Hpy8I GTNNAC 5 cut(s) 76, 112, 287, 419, 995
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 9 cut(s) 104, 483, 573, 806, 974, 1142, 1392, 1601, 1660
HpyCH4III ACNGT 6 cut(s) 416, 479, 787, 854, 1291, 1427
HpyCH4IV ACGT 3 cut(s) 72, 151, 1335
HpyCH4V TGCA 7 cut(s) 174, 987, 995, 1235, 1520, 1541, 1551
HpyF10VI GCNNNNNNNGC 3 cut(s) 650, 1293, 1517
HpyF3I CTNAG 6 cut(s) 281, 539, 637, 1027, 1212, 1662
HpySE526I ACGT 3 cut(s) 72, 151, 1335
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 9 cut(s) 279, 668, 746, 947, 1015, 1202, 1517, 1585, 1633
Kpn2I TCCGGA 1 cut(s) 406
Ksp22I TGATCA 3 cut(s) 730, 1147, 1626
Kzo9I GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
LmnI GCTCC 1 cut(s) 1391
Lsp1109I GCAGC 3 cut(s) 77, 141, 1264
LweI GCATC 5 cut(s) 183, 653, 974, 1359, 1498
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 9 cut(s) 99, 753, 768, 1047, 1281, 1373, 1395, 1493, 1529
MaeII ACGT 3 cut(s) 72, 151, 1335
MaeIII GTNAC 1 cut(s) 667
MalI GATC 5 cut(s) 732, 1149, 1157, 1217, 1628
MboI GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
MfeI CAATTG 2 cut(s) 45, 518
MflI RGATCY 2 cut(s) 1155, 1215
MhlI GDGCHC 1 cut(s) 997
MlyI GAGTC 1 cut(s) 21
MroI TCCGGA 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 1131
MseI TTAA 5 cut(s) 203, 434, 795, 948, 1422
MslI CAYNNNNRTG 2 cut(s) 948, 1546
MspI CCGG 4 cut(s) 341, 407, 422, 1565
MspR9I CCNGG 4 cut(s) 291, 570, 595, 1565
MunI CAATTG 2 cut(s) 45, 518
Mva1269I GAATGC 2 cut(s) 1551, 1605
MvaI CCWGG 3 cut(s) 291, 570, 595
MwoI GCNNNNNNNGC 3 cut(s) 650, 1293, 1517
NciI CCSGG 1 cut(s) 1565
NdeII GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
NlaIII CATG 9 cut(s) 279, 668, 746, 947, 1015, 1202, 1517, 1585, 1633
NlaIV GGNNCC 7 cut(s) 345, 494, 722, 925, 1157, 1404, 1562
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 279
OliI CACNNNNGTG 1 cut(s) 1546
PciI ACATGT 1 cut(s) 275
PctI GAATGC 2 cut(s) 1551, 1605
PdmI GAANNNNTTC 1 cut(s) 1131
PfeI GAWTC 5 cut(s) 4, 700, 1052, 1570, 1613
PkrI GCNGC 3 cut(s) 92, 131, 1279
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
PscI ACATGT 1 cut(s) 275
PshBI ATTAAT 1 cut(s) 434
PsiI TTATAA 2 cut(s) 507, 1455
Psp6I CCWGG 3 cut(s) 289, 568, 593
PspGI CCWGG 3 cut(s) 289, 568, 593
PspN4I GGNNCC 7 cut(s) 345, 494, 722, 925, 1157, 1404, 1562
PspPI GGNCC 2 cut(s) 368, 591
PsuI RGATCY 2 cut(s) 1155, 1215
RsaI GTAC 4 cut(s) 263, 420, 747, 1660
RsaNI GTAC 4 cut(s) 262, 419, 746, 1659
RseI CAYNNNNRTG 2 cut(s) 948, 1546
SaqAI TTAA 5 cut(s) 203, 434, 795, 948, 1422
SatI GCNGC 3 cut(s) 91, 130, 1278
Sau3AI GATC 5 cut(s) 730, 1147, 1155, 1215, 1626
Sau96I GGNCC 2 cut(s) 368, 591
ScaI AGTACT 1 cut(s) 1660
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 4 cut(s) 291, 570, 595, 1565
SduI GDGCHC 1 cut(s) 997
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 5 cut(s) 183, 653, 974, 1359, 1498
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 2 cut(s) 948, 1546
SpeI ACTAGT 1 cut(s) 1528
SsiI CCGC 1 cut(s) 485
SspI AATATT 1 cut(s) 191
SspMI CTAG 9 cut(s) 99, 753, 768, 1047, 1281, 1373, 1395, 1493, 1529
StyD4I CCNGG 4 cut(s) 289, 568, 593, 1563
StyI CCWWGG 2 cut(s) 1138, 1248
TaaI ACNGT 6 cut(s) 416, 479, 787, 854, 1291, 1427
TaiI ACGT 3 cut(s) 75, 154, 1338
TaqI TCGA 1 cut(s) 69
TatI WGTACW 2 cut(s) 261, 1658
TfiI GAWTC 5 cut(s) 4, 700, 1052, 1570, 1613
Tru1I TTAA 5 cut(s) 203, 434, 795, 948, 1422
Tru9I TTAA 5 cut(s) 203, 434, 795, 948, 1422
TscAI CASTG 2 cut(s) 1432, 1562
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 3 cut(s) 90, 129, 1277
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 7 cut(s) 69, 722, 1000, 1027, 1306, 1518, 1598
TspRI CASTG 2 cut(s) 1432, 1562
VneI GTGCAC 1 cut(s) 993
VpaK11BI GGWCC 1 cut(s) 368
VspI ATTAAT 1 cut(s) 434
XapI RAATTY 3 cut(s) 247, 457, 936
XbaI TCTAGA 1 cut(s) 1046
XceI RCATGY 1 cut(s) 279
XmiI GTMKAC 2 cut(s) 75, 286
XmnI GAANNNNTTC 1 cut(s) 1131
XspI CTAG 9 cut(s) 99, 753, 768, 1047, 1281, 1373, 1395, 1493, 1529
ZraI GACGTC 1 cut(s) 73
ZrmI AGTACT 1 cut(s) 1660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.