Rmu_sc0028145.1_g000001

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0028145.1
OG0000953
Physical Location & Seq
Reverse (-)
2182 .. 10797
8616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0028145.1_g000001.1.cds

Sequence Viewer

Length: 1617 bp
atgaatatcctgcttctgcggaagtctttgcttttcttgcttgtaattgtgttccccagtattgttgaaccaattagcaaagcaggcgatgatcaggactgcccagttcagaggtgtaggcatgatggtccccttatccagtttccatttcgtctacaacactatccccttcactgtggccatccagagtttgaggtttcctgcagcagcttcaccaacatgaccatgatgaagctctcctcatcatcaggattctttctaatacaaacaattgattacgagagacaacagtttcgtgtgtatgatgagggtggttgcctcccgagactccttcttaatcttactgctgtctccttctcgccctttcaactagcaccttatgacctgactttctgcggttcgtctggctatgggaacagccaactgatgctaaactcgacgttgttaaattgtaccacggcacaaaatttgagcgaatccatcacaccagatgacacctacttctggaaccggaagtgcgtggcgatcaaatgtctcagtgtcccagggcattatcaagttctggctactactgagtattttacaatgtctgacttgccagtagacagtccttgttctactttgtcagaactctatcttccaattcgacgtataaatgcagagtctagttgtgaaccagacactagtttattgctggaatggagtgaacggaatttcccagtatgtcaaatccccagacacaaaggtctatcaactaggtttattgtcatggcagtgagcgtatgtggttttgttctcttgtcaaccacagcggtagccatcggttaccatttaaagcaatcaaagaggagagaggaaaaggagaatcaaagaaagattgaaaagttccttgatgatcacaagtcccatgtaccaacaagatactcctacgctgatataaagaagatgacaaatgggttcaagaagaagttgggtgaaggaggttttggaagtgttttctgtggaaagcttcccactaatggagtgccagtggctgtaaaagtcctccaggattctaaaggaagtggagaagattttgttaacgaagtgggaaccattggcagaattcaccatgtcaacgtggttcgactccttgggttttcagctgaaggagggaagcgtgctcttatttatgagttcatgtcaaacaggtccctagagaagttcatcaaatctagaggatcaaataatagcagcagttcatttaactgggagaaactttgcaacatcatcacaggtatagcaaagggaatcgagtaccttccccaagggtgtgaccagaggattctccactttgatatcaagcctcacaatatcttgttagaccatgcctttaatccaaaaatttccgattttggtctggctaaactctgttccaacgaagatagtgtcatatctatgactgctgccagaggcactgtaggctacattgcacctgaagtatttaatggcaattttggaactgtcacctacaaatctgatgtgtttagtttcgggatgttggttcttgaacaatcagggggagatatcgacatcagggggagttatgatgtctacatgttcgatctcgaagagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

538

Amino Acids

60.19

Weight (kDa)

6.71

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 744
AccI GTMKAC 3 cut(s) 154, 603, 1593
AciI CCGC 3 cut(s) 19, 396, 812
AclWI GGATC 1 cut(s) 1228
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 4 cut(s) 466, 712, 1104, 1383
AcuI CTGAAG 2 cut(s) 1167, 1497
AfaI GTAC 3 cut(s) 454, 912, 1298
AfiI CCNNNNNNNGG 2 cut(s) 504, 1019
AflIII ACRYGT 1 cut(s) 1596
AgsI TTSAA 5 cut(s) 68, 368, 881, 961, 1550
AhlI ACTAGT 1 cut(s) 683
AjnI CCWGG 2 cut(s) 544, 1047
AjuI GAANNNNNNNTTGG 2 cut(s) 969, 1001
AloI GAACNNNNNNTCC 2 cut(s) 699, 731
AluBI AGCT 4 cut(s) 210, 235, 1009, 1145
AluI AGCT 4 cut(s) 210, 235, 1009, 1145
Alw21I GWGCWC 1 cut(s) 1165
Alw26I GTCTC 4 cut(s) 277, 319, 355, 539
AlwI GGATC 1 cut(s) 1228
AlwNI CAGNNNCTG 1 cut(s) 1034
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 4 cut(s) 204, 207, 1233, 1445
ApoI RAATTY 4 cut(s) 466, 712, 1104, 1383
ArsI GACNNNNNNTTYG 4 cut(s) 276, 308, 1413, 1445
AspS9I GGNCC 2 cut(s) 128, 1191
AsuHPI GGTGA 4 cut(s) 205, 986, 1100, 1498
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 2 cut(s) 128, 1191
BalI TGGCCA 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 1165
BbvI GCAGC 4 cut(s) 216, 219, 1245, 1432
BccI CCATC 4 cut(s) 119, 189, 488, 827
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 2 cut(s) 546, 1049
BclI TGATCA 2 cut(s) 91, 895
BcoDI GTCTC 4 cut(s) 277, 319, 355, 539
BcuI ACTAGT 1 cut(s) 683
BfaI CTAG 6 cut(s) 371, 666, 684, 756, 1196, 1215
BfmI CTRYAG 2 cut(s) 202, 1458
BisI GCNGC 4 cut(s) 205, 208, 1234, 1446
BlsI GCNGC 4 cut(s) 206, 209, 1235, 1447
Bme1390I CCNGG 2 cut(s) 546, 1049
Bme18I GGWCC 2 cut(s) 128, 1191
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 128, 1191
BmiI GGNNCC 4 cut(s) 130, 509, 1093, 1193
BmrFI CCNGG 2 cut(s) 546, 1049
BmrI ACTGGG 4 cut(s) 51, 98, 713, 1258
BmsI GCATC 1 cut(s) 417
BmuI ACTGGG 4 cut(s) 51, 98, 713, 1258
BpmI CTGGAG 1 cut(s) 1031
BsaJI CCNNGG 5 cut(s) 456, 544, 545, 1132, 1306
BsaWI WCCGGW 1 cut(s) 510
BsaXI ACNNNNNCTCC 2 cut(s) 1014, 1044
Bsc4I CCNNNNNNNGG 2 cut(s) 504, 1019
Bse1I ACTGG 7 cut(s) 57, 104, 139, 599, 719, 1028, 1253
Bse3DI GCAATG 1 cut(s) 1467
BseBI CCWGG 2 cut(s) 546, 1049
BseDI CCNNGG 5 cut(s) 456, 544, 545, 1132, 1306
BseGI GGATG 2 cut(s) 181, 1542
BseLI CCNNNNNNNGG 2 cut(s) 504, 1019
BseMI GCAATG 1 cut(s) 1467
BseMII CTCAG 2 cut(s) 550, 564
BseNI ACTGG 7 cut(s) 57, 104, 139, 599, 719, 1028, 1253
BseRI GAGGAG 2 cut(s) 229, 862
BseXI GCAGC 4 cut(s) 216, 219, 1245, 1432
BshFI GGCC 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 1165
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 1 cut(s) 511
BslFI GGGAC 4 cut(s) 114, 527, 889, 1177
BslI CCNNNNNNNGG 2 cut(s) 504, 1019
BsmAI GTCTC 4 cut(s) 277, 319, 355, 539
BsmFI GGGAC 4 cut(s) 114, 527, 889, 1177
BsnI GGCC 1 cut(s) 180
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 1165
Bsp143I GATC 5 cut(s) 91, 525, 895, 1220, 1603
BspACI CCGC 3 cut(s) 19, 396, 812
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 2 cut(s) 549, 565
BspLI GGNNCC 4 cut(s) 130, 509, 1093, 1193
BspMAI CTGCAG 1 cut(s) 206
BspPI GGATC 1 cut(s) 1228
BsrDI GCAATG 1 cut(s) 1467
BsrI ACTGG 7 cut(s) 57, 104, 139, 599, 719, 1028, 1253
BssECI CCNNGG 5 cut(s) 456, 544, 545, 1132, 1306
BssMI GATC 5 cut(s) 91, 525, 895, 1220, 1603
BssT1I CCWWGG 2 cut(s) 1132, 1306
Bst2UI CCWGG 2 cut(s) 546, 1049
Bst4CI ACNGT 5 cut(s) 176, 291, 608, 1459, 1504
Bst6I CTCTTC 1 cut(s) 1605
BstC8I GCNNGC 2 cut(s) 85, 1161
BstDEI CTNAG 2 cut(s) 536, 573
BstDSI CCRYGG 1 cut(s) 456
BstEII GGTNACC 1 cut(s) 824
BstF5I GGATG 2 cut(s) 181, 1542
BstKTI GATC 5 cut(s) 94, 528, 898, 1223, 1606
BstMAI GTCTC 4 cut(s) 277, 319, 355, 539
BstMBI GATC 5 cut(s) 91, 525, 895, 1220, 1603
BstMWI GCNNNNNNNGC 3 cut(s) 37, 84, 1461
BstNI CCWGG 2 cut(s) 546, 1049
BstNSI RCATGY 1 cut(s) 1600
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 2 cut(s) 544, 1047
BstSFI CTRYAG 2 cut(s) 202, 1458
BstV1I GCAGC 4 cut(s) 216, 219, 1245, 1432
BsuRI GGCC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 456
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 2 cut(s) 181, 1542
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 5 cut(s) 172, 544, 780, 1035, 1455
Cac8I GCNNGC 2 cut(s) 85, 1161
CaiI CAGNNNCTG 1 cut(s) 1034
Cfr13I GGNCC 2 cut(s) 128, 1191
Csp6I GTAC 3 cut(s) 453, 911, 1297
CviAII CATG 9 cut(s) 122, 220, 226, 769, 908, 1112, 1180, 1367, 1597
CviQI GTAC 3 cut(s) 453, 911, 1297
DdeI CTNAG 2 cut(s) 536, 573
DpnI GATC 5 cut(s) 93, 527, 897, 1222, 1605
DpnII GATC 5 cut(s) 91, 525, 895, 1220, 1603
DraI TTTAAA 1 cut(s) 834
DrdI GACNNNNNNGTC 1 cut(s) 744
DseDI GACNNNNNNGTC 1 cut(s) 744
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 1605
EarI CTCTTC 1 cut(s) 1605
Eco130I CCWWGG 2 cut(s) 1132, 1306
Eco32I GATATC 2 cut(s) 1339, 1567
Eco47I GGWCC 2 cut(s) 128, 1191
Eco57I CTGAAG 2 cut(s) 1167, 1497
Eco88I CYCGRG 1 cut(s) 322
Eco91I GGTNACC 1 cut(s) 824
EcoO109I RGGNCCY 1 cut(s) 1191
EcoO65I GGTNACC 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 1104
EcoRII CCWGG 2 cut(s) 544, 1047
EcoRV GATATC 2 cut(s) 1339, 1567
EcoT14I CCWWGG 2 cut(s) 1132, 1306
ErhI CCWWGG 2 cut(s) 1132, 1306
FaeI CATG 9 cut(s) 125, 223, 229, 772, 911, 1115, 1183, 1370, 1600
FaqI GGGAC 4 cut(s) 114, 527, 889, 1177
FatI CATG 9 cut(s) 121, 219, 225, 768, 907, 1111, 1179, 1366, 1596
FbaI TGATCA 2 cut(s) 91, 895
FblI GTMKAC 3 cut(s) 154, 603, 1593
Fnu4HI GCNGC 4 cut(s) 205, 208, 1234, 1446
FokI GGATG 2 cut(s) 168, 1549
Fsp4HI GCNGC 4 cut(s) 205, 208, 1234, 1446
FspBI CTAG 6 cut(s) 371, 666, 684, 756, 1196, 1215
GluI GCNGC 4 cut(s) 205, 208, 1234, 1446
GsuI CTGGAG 1 cut(s) 1031
HaeIII GGCC 1 cut(s) 180
HapII CCGG 1 cut(s) 511
Hin1II CATG 9 cut(s) 125, 223, 229, 772, 911, 1115, 1183, 1370, 1600
HincII GTYRAC 3 cut(s) 804, 1081, 1117
HindII GTYRAC 3 cut(s) 804, 1081, 1117
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 9 cut(s) 252, 327, 476, 662, 865, 1052, 1128, 1290, 1324
HpaI GTTAAC 1 cut(s) 1081
HpaII CCGG 1 cut(s) 511
HphI GGTGA 4 cut(s) 205, 986, 1100, 1498
Hpy166II GTNNAC 8 cut(s) 155, 604, 674, 707, 804, 1081, 1117, 1594
Hpy188I TCNGA 5 cut(s) 111, 592, 628, 1390, 1519
Hpy8I GTNNAC 8 cut(s) 155, 604, 674, 707, 804, 1081, 1117, 1594
Hpy99I CGWCG 2 cut(s) 442, 651
HpyAV CCTTC 6 cut(s) 179, 341, 364, 971, 1142, 1310
HpyCH4III ACNGT 5 cut(s) 176, 291, 608, 1459, 1504
HpyCH4IV ACGT 3 cut(s) 440, 649, 1119
HpyCH4V TGCA 4 cut(s) 204, 659, 1263, 1472
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 84, 1461
HpyF3I CTNAG 2 cut(s) 536, 573
HpySE526I ACGT 3 cut(s) 440, 649, 1119
Hsp92II CATG 9 cut(s) 125, 223, 229, 772, 911, 1115, 1183, 1370, 1600
Ksp22I TGATCA 2 cut(s) 91, 895
KspAI GTTAAC 1 cut(s) 1081
Kzo9I GATC 5 cut(s) 91, 525, 895, 1220, 1603
Lsp1109I GCAGC 4 cut(s) 216, 219, 1245, 1432
LweI GCATC 1 cut(s) 417
MaeI CTAG 6 cut(s) 371, 666, 684, 756, 1196, 1215
MaeII ACGT 3 cut(s) 440, 649, 1119
MaeIII GTNAC 3 cut(s) 824, 1313, 1504
MalI GATC 5 cut(s) 93, 527, 897, 1222, 1605
MboI GATC 5 cut(s) 91, 525, 895, 1220, 1603
MboII GAAGA 5 cut(s) 629, 955, 976, 1082, 1433
MfeI CAATTG 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 1165
MlsI TGGCCA 1 cut(s) 180
MluNI TGGCCA 1 cut(s) 180
MlyI GAGTC 3 cut(s) 321, 671, 1122
MmeI TCCRAC 1 cut(s) 1440
Mox20I TGGCCA 1 cut(s) 180
MscI TGGCCA 1 cut(s) 180
MseI TTAA 7 cut(s) 336, 446, 833, 1080, 1245, 1374, 1485
MslI CAYNNNNRTG 4 cut(s) 218, 224, 773, 1436
Msp20I TGGCCA 1 cut(s) 180
MspA1I CMGCKG 2 cut(s) 812, 1145
MspI CCGG 1 cut(s) 511
MspR9I CCNGG 2 cut(s) 546, 1049
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 2 cut(s) 546, 1049
MwoI GCNNNNNNNGC 3 cut(s) 37, 84, 1461
NdeII GATC 5 cut(s) 91, 525, 895, 1220, 1603
NlaIII CATG 9 cut(s) 125, 223, 229, 772, 911, 1115, 1183, 1370, 1600
NlaIV GGNNCC 4 cut(s) 130, 509, 1093, 1193
NmuCI GTSAC 2 cut(s) 1313, 1504
NspI RCATGY 1 cut(s) 1600
PasI CCCWGGG 1 cut(s) 545
PciI ACATGT 1 cut(s) 1596
PfeI GAWTC 6 cut(s) 252, 476, 865, 1052, 1290, 1324
PfoI TCCNGGA 1 cut(s) 1047
PkrI GCNGC 4 cut(s) 206, 209, 1235, 1447
PleI GAGTC 3 cut(s) 321, 670, 1122
PpsI GAGTC 3 cut(s) 321, 670, 1122
PpuMI RGGWCCY 1 cut(s) 1191
PscI ACATGT 1 cut(s) 1596
Psp5II RGGWCCY 1 cut(s) 1191
Psp6I CCWGG 2 cut(s) 544, 1047
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 2 cut(s) 544, 1047
PspN4I GGNNCC 4 cut(s) 130, 509, 1093, 1193
PspPI GGNCC 2 cut(s) 128, 1191
PspPPI RGGWCCY 1 cut(s) 1191
PstI CTGCAG 1 cut(s) 206
PstNI CAGNNNCTG 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 3 cut(s) 454, 912, 1298
RsaNI GTAC 3 cut(s) 453, 911, 1297
RseI CAYNNNNRTG 4 cut(s) 218, 224, 773, 1436
SaqAI TTAA 7 cut(s) 336, 446, 833, 1080, 1245, 1374, 1485
SatI GCNGC 4 cut(s) 205, 208, 1234, 1446
Sau3AI GATC 5 cut(s) 91, 525, 895, 1220, 1603
Sau96I GGNCC 2 cut(s) 128, 1191
SchI GAGTC 3 cut(s) 321, 671, 1122
ScrFI CCNGG 2 cut(s) 546, 1049
SduI GDGCHC 1 cut(s) 1165
SfaNI GCATC 1 cut(s) 417
SfcI CTRYAG 2 cut(s) 202, 1458
SinI GGWCC 2 cut(s) 128, 1191
SmiMI CAYNNNNRTG 4 cut(s) 218, 224, 773, 1436
SpeI ACTAGT 1 cut(s) 683
SsiI CCGC 3 cut(s) 19, 396, 812
SspMI CTAG 6 cut(s) 371, 666, 684, 756, 1196, 1215
StyD4I CCNGG 2 cut(s) 544, 1047
StyI CCWWGG 2 cut(s) 1132, 1306
TaaI ACNGT 5 cut(s) 176, 291, 608, 1459, 1504
TaiI ACGT 3 cut(s) 443, 652, 1122
TaqI TCGA 7 cut(s) 437, 646, 1126, 1293, 1569, 1602, 1608
TfiI GAWTC 6 cut(s) 252, 476, 865, 1052, 1290, 1324
Tru1I TTAA 7 cut(s) 336, 446, 833, 1080, 1245, 1374, 1485
Tru9I TTAA 7 cut(s) 336, 446, 833, 1080, 1245, 1374, 1485
TscAI CASTG 5 cut(s) 179, 544, 780, 1035, 1462
TseFI GTSAC 2 cut(s) 1313, 1504
TseI GCWGC 4 cut(s) 204, 207, 1233, 1445
Tsp45I GTSAC 2 cut(s) 1313, 1504
TspDTI ATGAA 5 cut(s) 17, 245, 1168, 1195, 1230
TspGWI ACGGA 1 cut(s) 724
TspRI CASTG 5 cut(s) 179, 544, 780, 1035, 1462
VpaK11BI GGWCC 2 cut(s) 128, 1191
XapI RAATTY 4 cut(s) 466, 712, 1104, 1383
XbaI TCTAGA 1 cut(s) 1214
XceI RCATGY 1 cut(s) 1600
XmiI GTMKAC 3 cut(s) 154, 603, 1593
XspI CTAG 6 cut(s) 371, 666, 684, 756, 1196, 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.