RchiOBHm_Chr3g0449341

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000953
Physical Location & Seq
Forward (+)
1166717 .. 1167631
915 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41670

Sequence Viewer

Length: 915 bp
ATGACAAATGAATTCAAGAAGAAGTTAGGTGAAGGCGGTTTTGGAAGTGTTTTCAGTGGAAAGCTTCCCAATAATGGAGTGCCAGTTGCAGTAAAAGTCCTCAAGGATTCTAAAGGAAGTGGAGAAGATTTTGTTAACGAAGTGGGAACCATTGGCAGGATTCACCATGTAAACGTGGTTCGCCTACTTGGGTTTTCAGCTGAAGGAGGGAACCGTGCTCTTATTTTTGAGCTCATGCCAAACAGGTCCCTAGAGAAGTTCATCAAATCTACAGGATCAAATAAGAGCAGGAGTTCATTTAACTGGGAGAAACTTTGCAACATCATCACAGGTATAGCAAAGGGAATCGAGTACCTTCACCAAGGGTGTGACCAGAGGATTCTCCACTTTGATATCAAGCCTCACAATATCTTGTTAGACCATGCCTTTAATCCGAAAATTTCTGATTTTGGTATGGCTAAACTCTGTTCCAAGGAAGACAGTGCTATATCTATGACTGCTGCCAGAGGCACTATAGGCTACATTGCACCTGAAGTATTTAATGGAAACTTTGGAACTGTCTCCCACAAATCGGATGTGTATAGTTTCGGGATGCTTGTTCTTGAAGTTATTGGAGCTAGGAGGGAAAATGCGGTTACATCTAGTGACAATGAGGTGTATTTTCCTGAATTGATTTACAAGTGTCTGATCCAAGGAGAAGAGTTGGATTTGGAGCTAGCTGATGATGGAGATGCTCAGATTGCTAAGAGATTAGCAATTGTGGCACTCTGGTGCATCCAGTGGTACCCGGTGAATCGCCCTTCCATGAAAGAAGTTGTTATAATGCTGGAAGGACCCTCCGAAAGCTTGATCATGCCACCCAATCCGTTTGCTTCCACAACTCAGATGGATCCAACAACAACCCCATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

304

Amino Acids

33.36

Weight (kDa)

6.01

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 4 - 274 7.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 5 - 273 1.3e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
Acc65I GGTACC 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 783
AciI CCGC 2 cut(s) 36, 632
AclWI GGATC 4 cut(s) 283, 682, 884, 897
AcsI RAATTY 2 cut(s) 11, 438
AcuI CTGAAG 2 cut(s) 222, 552
AfaI GTAC 2 cut(s) 353, 785
AfiI CCNNNNNNNGG 3 cut(s) 74, 156, 571
AgsI TTSAA 2 cut(s) 16, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AleI CACNNNNGTG 1 cut(s) 769
AluBI AGCT 7 cut(s) 64, 200, 232, 617, 715, 719, 846
AluI AGCT 7 cut(s) 64, 200, 232, 617, 715, 719, 846
Alw21I GWGCWC 2 cut(s) 220, 234
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 4 cut(s) 283, 682, 884, 897
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 2 cut(s) 11, 438
Asp718I GGTACC 1 cut(s) 783
AspS9I GGNCC 2 cut(s) 246, 833
AsuC2I CCSGG 1 cut(s) 788
AsuHPI GGTGA 4 cut(s) 41, 155, 350, 802
AsuNHI GCTAGC 1 cut(s) 715
AvaII GGWCC 2 cut(s) 246, 833
BamHI GGATCC 1 cut(s) 889
BanI GGYRCC 1 cut(s) 783
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 2 cut(s) 220, 234
BbvI GCAGC 1 cut(s) 487
BccI CCATC 3 cut(s) 719, 880, 913
BclI TGATCA 1 cut(s) 849
BcnI CCSGG 1 cut(s) 788
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 4 cut(s) 251, 618, 642, 716
BfmI CTRYAG 2 cut(s) 270, 513
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 1 cut(s) 788
Bme18I GGWCC 2 cut(s) 246, 833
BmgT120I GGNCC 2 cut(s) 246, 833
BmiI GGNNCC 6 cut(s) 148, 212, 248, 785, 835, 891
BmrFI CCNGG 1 cut(s) 788
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 3 cut(s) 582, 721, 783
BmtI GCTAGC 1 cut(s) 719
BmuI ACTGGG 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 788
BsaJI CCNNGG 3 cut(s) 361, 471, 691
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 156, 571
Bse1I ACTGG 3 cut(s) 83, 308, 778
Bse3DI GCAATG 1 cut(s) 522
BseDI CCNNGG 3 cut(s) 361, 471, 691
BseGI GGATG 3 cut(s) 580, 597, 774
BseLI CCNNNNNNNGG 3 cut(s) 74, 156, 571
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 2 cut(s) 749, 896
BseNI ACTGG 3 cut(s) 83, 308, 778
BseXI GCAGC 1 cut(s) 487
BshNI GGYRCC 1 cut(s) 783
BsiHKAI GWGCWC 2 cut(s) 220, 234
BsiSI CCGG 1 cut(s) 788
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 3 cut(s) 74, 156, 571
BsmAI GTCTC 1 cut(s) 565
BsmFI GGGAC 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 220, 234
Bsp143I GATC 4 cut(s) 275, 687, 849, 889
BspACI CCGC 2 cut(s) 36, 632
BspCNI CTCAG 2 cut(s) 748, 895
BspLI GGNNCC 6 cut(s) 148, 212, 248, 785, 835, 891
BspOI GCTAGC 1 cut(s) 719
BspPI GGATC 4 cut(s) 283, 682, 884, 897
BspT107I GGYRCC 1 cut(s) 783
BsrDI GCAATG 1 cut(s) 522
BsrI ACTGG 3 cut(s) 83, 308, 778
BssECI CCNNGG 3 cut(s) 361, 471, 691
BssMI GATC 4 cut(s) 275, 687, 849, 889
BssT1I CCWWGG 3 cut(s) 361, 471, 691
Bst4CI ACNGT 3 cut(s) 215, 482, 559
Bst6I CTCTTC 1 cut(s) 693
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 3 cut(s) 735, 744, 882
BstF5I GGATG 3 cut(s) 580, 597, 774
BstKTI GATC 4 cut(s) 278, 690, 852, 892
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 4 cut(s) 275, 687, 849, 889
BstMWI GCNNNNNNNGC 3 cut(s) 516, 740, 761
BstSCI CCNGG 1 cut(s) 786
BstSFI CTRYAG 2 cut(s) 270, 513
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BtsCI GGATG 3 cut(s) 580, 597, 774
BtsIMutI CAGTG 3 cut(s) 61, 487, 785
Cac8I GCNNGC 1 cut(s) 717
Cfr13I GGNCC 2 cut(s) 246, 833
Csp6I GTAC 2 cut(s) 352, 784
CviAII CATG 5 cut(s) 167, 235, 422, 805, 853
CviQI GTAC 2 cut(s) 352, 784
DdeI CTNAG 3 cut(s) 735, 744, 882
DpnI GATC 4 cut(s) 277, 689, 851, 891
DpnII GATC 4 cut(s) 275, 687, 849, 889
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 361, 471, 691
Eco24I GRGCYC 1 cut(s) 234
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 2 cut(s) 246, 833
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 222, 552
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 2 cut(s) 246, 833
EcoRI GAATTC 1 cut(s) 11
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 3 cut(s) 361, 471, 691
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 3 cut(s) 361, 471, 691
FaeI CATG 5 cut(s) 170, 238, 425, 808, 856
FaqI GGGAC 1 cut(s) 232
FatI CATG 5 cut(s) 166, 234, 421, 804, 852
FbaI TGATCA 1 cut(s) 849
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 3 cut(s) 587, 604, 761
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 4 cut(s) 251, 618, 642, 716
GluI GCNGC 1 cut(s) 501
HapII CCGG 1 cut(s) 788
Hin1II CATG 5 cut(s) 170, 238, 425, 808, 856
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 62, 844
HinfI GANTC 5 cut(s) 107, 160, 345, 379, 793
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 1 cut(s) 788
HphI GGTGA 4 cut(s) 41, 155, 350, 802
Hpy166II GTNNAC 2 cut(s) 136, 172
Hpy188I TCNGA 7 cut(s) 435, 445, 574, 687, 738, 841, 885
Hpy188III TCNNGA 4 cut(s) 16, 589, 602, 665
Hpy8I GTNNAC 2 cut(s) 136, 172
HpyAV CCTTC 5 cut(s) 26, 197, 365, 810, 824
HpyCH4III ACNGT 3 cut(s) 215, 482, 559
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 89, 318, 527, 774
HpyF10VI GCNNNNNNNGC 3 cut(s) 516, 740, 761
HpyF3I CTNAG 3 cut(s) 735, 744, 882
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 5 cut(s) 170, 238, 425, 808, 856
KpnI GGTACC 1 cut(s) 787
Ksp22I TGATCA 1 cut(s) 849
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 4 cut(s) 275, 687, 849, 889
LmnI GCTCC 2 cut(s) 614, 712
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 3 cut(s) 582, 721, 783
MaeI CTAG 4 cut(s) 251, 618, 642, 716
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 368, 634, 644
MalI GATC 4 cut(s) 277, 689, 851, 891
MboI GATC 4 cut(s) 275, 687, 849, 889
MboII GAAGA 4 cut(s) 31, 137, 488, 710
MfeI CAATTG 1 cut(s) 756
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 2 cut(s) 220, 234
MluCI AATT 4 cut(s) 11, 438, 668, 756
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 8 cut(s) 110, 200, 369, 411, 500, 615, 646, 847
MseI TTAA 5 cut(s) 135, 300, 429, 540, 913
MslI CAYNNNNRTG 1 cut(s) 769
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 788
MspR9I CCNGG 1 cut(s) 788
MunI CAATTG 1 cut(s) 756
MwoI GCNNNNNNNGC 3 cut(s) 516, 740, 761
NciI CCSGG 1 cut(s) 788
NdeII GATC 4 cut(s) 275, 687, 849, 889
NheI GCTAGC 1 cut(s) 715
NlaIII CATG 5 cut(s) 170, 238, 425, 808, 856
NlaIV GGNNCC 6 cut(s) 148, 212, 248, 785, 835, 891
NmuCI GTSAC 2 cut(s) 368, 644
OliI CACNNNNGTG 1 cut(s) 769
PfeI GAWTC 5 cut(s) 107, 160, 345, 379, 793
PkrI GCNGC 1 cut(s) 502
PpuMI RGGWCCY 2 cut(s) 246, 833
PsiI TTATAA 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 2 cut(s) 246, 833
PspN4I GGNNCC 6 cut(s) 148, 212, 248, 785, 835, 891
PspPI GGNCC 2 cut(s) 246, 833
PspPPI RGGWCCY 2 cut(s) 246, 833
PsrI GAACNNNNNNTAC 2 cut(s) 162, 194
PsuI RGATCY 1 cut(s) 889
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 2 cut(s) 353, 785
RsaNI GTAC 2 cut(s) 352, 784
RseI CAYNNNNRTG 1 cut(s) 769
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 5 cut(s) 135, 300, 429, 540, 913
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 4 cut(s) 275, 687, 849, 889
Sau96I GGNCC 2 cut(s) 246, 833
ScrFI CCNGG 1 cut(s) 788
SduI GDGCHC 2 cut(s) 220, 234
SfaNI GCATC 3 cut(s) 582, 721, 783
SfcI CTRYAG 2 cut(s) 270, 513
SinI GGWCC 2 cut(s) 246, 833
SmiMI CAYNNNNRTG 1 cut(s) 769
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 4 cut(s) 11, 438, 668, 756
SsiI CCGC 2 cut(s) 36, 632
SspMI CTAG 4 cut(s) 251, 618, 642, 716
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 786
StyI CCWWGG 3 cut(s) 361, 471, 691
TaaI ACNGT 3 cut(s) 215, 482, 559
TaiI ACGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 348
TasI AATT 4 cut(s) 11, 438, 668, 756
TfiI GAWTC 5 cut(s) 107, 160, 345, 379, 793
Tru1I TTAA 5 cut(s) 135, 300, 429, 540, 913
Tru9I TTAA 5 cut(s) 135, 300, 429, 540, 913
TscAI CASTG 3 cut(s) 61, 487, 785
TseFI GTSAC 2 cut(s) 368, 644
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 2 cut(s) 368, 644
TspDTI ATGAA 4 cut(s) 24, 250, 285, 821
TspGWI ACGGA 1 cut(s) 855
TspRI CASTG 3 cut(s) 61, 487, 785
VpaK11BI GGWCC 2 cut(s) 246, 833
XapI RAATTY 2 cut(s) 11, 438
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XspI CTAG 4 cut(s) 251, 618, 642, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.