pycom12g23110

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000953
Physical Location & Seq
Forward (+)
23770345 .. 23771181
837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23110.1

Sequence Viewer

Length: 837 bp
ATGGATACTCTGCTTCTGCCACTATTTTTCTTGCTTATAATTGTCTTCCCAATGGCCATGATTGTACCCAGCAGAGCAGCTGATCCTCATGACTGCCCGGTTACAAGGTGTAGGCCTGATGGTCCAGTTATCCGGTTTCCATTCCGTCTAAAACACCATCCTCCTTACTGTGGCCATCCAGAGTTTGAAGTTTTCTGCACACAAAATATGACCATGATCAAGCTCTCGCCGTCTTCGGCACCCTTTCCTATACAATCTATTGATTACCAAAAAAATCAGTTTCAGGTCTACGATGCGGATGGTTGCCTCCCGAAACACCTTCTTAATTTTACCATCTCCATATACTCACTCTTTCAACCAGTACGCAATTTTGTGTTTTACGACAGTTGCGGTTTTGATCTCGAATATTATCCAGATTTATTGAGCGTAACACTTTTAAATTGTAGCACAGCACAAAGTTCTCGCACAGAGAGCTCCCGTCCGTACTTCATGCCGATTAAATGTCTAAGCGTCCCGGGGAATCATCAAGTTCTAGCTGCTCTACAGACTACTACTGTAGATAAACTGCCGGTGTCTACTTACACTCTTCTGAGACAGCTTGATCTTCCAATACGAAATGTGGACGGTTCTATGCAAAACTGTGAACCGGGCAAGGTTTTATTCCTGCAATGGAAGGATTCATATTATCTGCGATTTACCTCATTGTCTATCCCTATCCCAGCATGTCAAAACTGCAGTACCAGTGAAGGCTGCTTTATTTATACCGACACAAATCAAATGCAGTGTTCTACTTATGACAGCCTTGAAATAGTCCCGAAAGGTACGGAGTTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

279

Amino Acids

31.61

Weight (kDa)

5.84

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 32 - 98 4.4e-15 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 238
AccI GTMKAC 2 cut(s) 288, 575
AciI CCGC 2 cut(s) 296, 390
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 2 cut(s) 54, 172
AfaI GTAC 5 cut(s) 66, 363, 485, 739, 823
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 3 cut(s) 188, 356, 806
AluBI AGCT 5 cut(s) 80, 223, 474, 536, 598
AluI AGCT 5 cut(s) 80, 223, 474, 536, 598
Alw21I GWGCWC 1 cut(s) 476
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 514
AoxI GGCC 3 cut(s) 54, 113, 172
ApeKI GCWGC 3 cut(s) 77, 536, 750
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 4 cut(s) 98, 515, 516, 648
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 1 cut(s) 122
BaeI ACNNNNGTAYC 2 cut(s) 721, 754
BalI TGGCCA 2 cut(s) 56, 174
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 476
BbsI GAAGAC 2 cut(s) 37, 225
Bbv12I GWGCWC 1 cut(s) 476
BbvI GCAGC 3 cut(s) 89, 523, 737
BccI CCATC 5 cut(s) 113, 165, 183, 293, 341
BceAI ACGGC 1 cut(s) 214
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 4 cut(s) 98, 515, 516, 648
BcoDI GTCTC 1 cut(s) 586
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 3 cut(s) 542, 555, 733
BisI GCNGC 3 cut(s) 78, 537, 751
BlsI GCNGC 3 cut(s) 79, 538, 752
Bme1390I CCNGG 4 cut(s) 98, 515, 516, 648
Bme18I GGWCC 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 4 cut(s) 98, 515, 516, 648
BmsI GCATC 1 cut(s) 283
BpiI GAAGAC 2 cut(s) 37, 225
BpuMI CCSGG 4 cut(s) 98, 515, 516, 648
BsaJI CCNNGG 2 cut(s) 514, 515
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 3 cut(s) 125, 359, 741
Bse3DI GCAATG 1 cut(s) 674
BseDI CCNNGG 2 cut(s) 514, 515
BseGI GGATG 3 cut(s) 157, 175, 304
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 3 cut(s) 125, 359, 741
BseXI GCAGC 3 cut(s) 89, 523, 737
BseYI CCCAGC 2 cut(s) 68, 718
BsgI GTGCAG 1 cut(s) 181
BshFI GGCC 3 cut(s) 56, 115, 174
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 476
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 5 cut(s) 98, 133, 515, 569, 647
BslFI GGGAC 2 cut(s) 497, 797
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 586
BsmFI GGGAC 2 cut(s) 497, 797
BsnI GGCC 3 cut(s) 56, 115, 174
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 4 cut(s) 82, 216, 397, 601
BspACI CCGC 2 cut(s) 296, 390
BspANI GGCC 3 cut(s) 56, 115, 174
BspCNI CTCAG 1 cut(s) 582
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 737
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 568
BsrI ACTGG 3 cut(s) 125, 359, 741
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 2 cut(s) 514, 515
BssMI GATC 4 cut(s) 82, 216, 397, 601
Bst4CI ACNGT 5 cut(s) 170, 386, 556, 626, 641
Bst6I CTCTTC 1 cut(s) 591
BstDEI CTNAG 2 cut(s) 506, 590
BstF5I GGATG 3 cut(s) 157, 175, 304
BstKTI GATC 4 cut(s) 85, 219, 400, 604
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 4 cut(s) 82, 216, 397, 601
BstMWI GCNNNNNNNGC 1 cut(s) 471
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 4 cut(s) 96, 513, 514, 646
BstSFI CTRYAG 3 cut(s) 542, 555, 733
BstV1I GCAGC 3 cut(s) 89, 523, 737
BstV2I GAAGAC 2 cut(s) 37, 225
BsuRI GGCC 3 cut(s) 56, 115, 174
BtsCI GGATG 3 cut(s) 157, 175, 304
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 2 cut(s) 748, 788
CciI TCATGA 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 122
Cfr9I CCCGGG 1 cut(s) 514
CseI GACGC 1 cut(s) 499
Csp6I GTAC 5 cut(s) 65, 362, 484, 738, 822
CviAII CATG 5 cut(s) 58, 89, 214, 490, 723
CviQI GTAC 5 cut(s) 65, 362, 484, 738, 822
DdeI CTNAG 2 cut(s) 506, 590
DpnI GATC 4 cut(s) 84, 218, 399, 603
DpnII GATC 4 cut(s) 82, 216, 397, 601
DraI TTTAAA 1 cut(s) 438
EaeI YGGCCR 2 cut(s) 54, 172
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Ecl136II GAGCTC 1 cut(s) 474
Eco147I AGGCCT 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 476
FaeI CATG 5 cut(s) 61, 92, 217, 493, 726
FaqI GGGAC 2 cut(s) 497, 797
FatI CATG 5 cut(s) 57, 88, 213, 489, 722
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 2 cut(s) 288, 575
Fnu4HI GCNGC 3 cut(s) 78, 537, 751
FokI GGATG 3 cut(s) 144, 162, 311
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 3 cut(s) 78, 537, 751
FspBI CTAG 1 cut(s) 533
GluI GCNGC 3 cut(s) 78, 537, 751
GsaI CCCAGC 2 cut(s) 72, 722
HaeIII GGCC 3 cut(s) 56, 115, 174
HapII CCGG 5 cut(s) 98, 133, 515, 569, 647
HgaI GACGC 1 cut(s) 499
Hin1II CATG 5 cut(s) 61, 92, 217, 493, 726
HinfI GANTC 2 cut(s) 520, 677
HpaII CCGG 5 cut(s) 98, 133, 515, 569, 647
Hpy166II GTNNAC 4 cut(s) 289, 576, 622, 644
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 6 cut(s) 89, 179, 310, 401, 413, 814
Hpy8I GTNNAC 4 cut(s) 289, 576, 622, 644
HpyAV CCTTC 3 cut(s) 329, 667, 740
HpyCH4III ACNGT 5 cut(s) 170, 386, 556, 626, 641
HpyCH4V TGCA 5 cut(s) 198, 634, 667, 735, 781
HpyF10VI GCNNNNNNNGC 1 cut(s) 471
HpyF3I CTNAG 2 cut(s) 506, 590
Hsp92II CATG 5 cut(s) 61, 92, 217, 493, 726
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 82, 216, 397, 601
LmnI GCTCC 1 cut(s) 479
Lsp1109I GCAGC 3 cut(s) 89, 523, 737
LweI GCATC 1 cut(s) 283
MaeI CTAG 1 cut(s) 533
MaeIII GTNAC 2 cut(s) 100, 427
MalI GATC 4 cut(s) 84, 218, 399, 603
MboI GATC 4 cut(s) 82, 216, 397, 601
MboII GAAGA 4 cut(s) 37, 225, 578, 596
MhlI GDGCHC 1 cut(s) 476
MlsI TGGCCA 2 cut(s) 56, 174
MluCI AATT 4 cut(s) 39, 325, 367, 439
MluNI TGGCCA 2 cut(s) 56, 174
MnlI CCTC 4 cut(s) 96, 171, 317, 709
Mox20I TGGCCA 2 cut(s) 56, 174
MscI TGGCCA 2 cut(s) 56, 174
MseI TTAA 3 cut(s) 324, 437, 498
Msp20I TGGCCA 2 cut(s) 56, 174
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 5 cut(s) 98, 133, 515, 569, 647
MspR9I CCNGG 4 cut(s) 98, 515, 516, 648
MwoI GCNNNNNNNGC 1 cut(s) 471
NciI CCSGG 4 cut(s) 98, 515, 516, 648
NdeII GATC 4 cut(s) 82, 216, 397, 601
NlaIII CATG 5 cut(s) 61, 92, 217, 493, 726
NlaIV GGNNCC 1 cut(s) 240
NspI RCATGY 1 cut(s) 726
PagI TCATGA 1 cut(s) 88
PceI AGGCCT 1 cut(s) 115
PfeI GAWTC 2 cut(s) 520, 677
PkrI GCNGC 3 cut(s) 79, 538, 752
PsiI TTATAA 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 476
PspFI CCCAGC 2 cut(s) 68, 718
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 1 cut(s) 737
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 5 cut(s) 66, 363, 485, 739, 823
RsaNI GTAC 5 cut(s) 65, 362, 484, 738, 822
SacI GAGCTC 1 cut(s) 476
SaqAI TTAA 3 cut(s) 324, 437, 498
SatI GCNGC 3 cut(s) 78, 537, 751
Sau3AI GATC 4 cut(s) 82, 216, 397, 601
Sau96I GGNCC 1 cut(s) 122
ScrFI CCNGG 4 cut(s) 98, 515, 516, 648
SduI GDGCHC 1 cut(s) 476
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 3 cut(s) 542, 555, 733
SinI GGWCC 1 cut(s) 122
SmaI CCCGGG 1 cut(s) 516
Sse9I AATT 4 cut(s) 39, 325, 367, 439
SseBI AGGCCT 1 cut(s) 115
SsiI CCGC 2 cut(s) 296, 390
SspI AATATT 1 cut(s) 407
SspMI CTAG 1 cut(s) 533
SstI GAGCTC 1 cut(s) 476
StuI AGGCCT 1 cut(s) 115
StyD4I CCNGG 4 cut(s) 96, 513, 514, 646
TaaI ACNGT 5 cut(s) 170, 386, 556, 626, 641
TaqI TCGA 1 cut(s) 402
TasI AATT 4 cut(s) 39, 325, 367, 439
TfiI GAWTC 2 cut(s) 520, 677
Tru1I TTAA 3 cut(s) 324, 437, 498
Tru9I TTAA 3 cut(s) 324, 437, 498
TscAI CASTG 2 cut(s) 748, 788
TseI GCWGC 3 cut(s) 77, 536, 750
TspDTI ATGAA 2 cut(s) 478, 669
TspGWI ACGGA 2 cut(s) 134, 471
TspMI CCCGGG 1 cut(s) 514
TspRI CASTG 2 cut(s) 748, 788
VpaK11BI GGWCC 1 cut(s) 122
XceI RCATGY 1 cut(s) 726
XmaI CCCGGG 1 cut(s) 514
XmiI GTMKAC 2 cut(s) 288, 575
XspI CTAG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.