RchiOBHm_Chr3g0449371

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000953
Physical Location & Seq
Forward (+)
1175459 .. 1177501
2043 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41673

Sequence Viewer

Length: 1911 bp
ATGGATATCCTGCCTCAGCTGAAGCCTCTGCTTTTCTTGCTTGTATTTGTGTTCCCCATTACTGTTGAACCAATTAGCAAATCAGCCGATGATCATGACTGCCCAGTTCAGAGGTGTAGGCATGATGGTCCCCTTATCCAGTTTCCCTTCCGTTTACAACACTATCCCCTTCACTGTGGCCATCCAGAGTTTGAGGTTTTCTGCAGCAGATTCACCAACACGACCATGATGAAGCTTTCCTCATCATCAGGATTCTTTCCAATACAAACAATCGATTACGAGGGACAACAGTTTCGTGTGCATGATGAGGATGGTTGCCTCCCGAGACGCCTTCTTAATTTTACTCTCTCCTCCTCGCTCTTTCAACCAGCACTTTTGACTGAAGATTCCGTTTGCAGTTACTCTTACATGACGTTGAGGAATATGACGTTGTTAAATTGTACGACGGCACAAAATTTTAGCACATTCACCACACCTGAGACCTACGACTGGAAGTACATCGCGATCAAATGTCTCAGTGTCCCAGGGCATCAAGTTCTGGCTACTACTCCGCACTTTACTATAAGTGACTTGCCGGTAGACGCTTGCTCTACTTTGTCAGAACTCTTTCTTCCATTTCAACCTATACAGGGTGATTATATGTATGCTTGTGAACTAGACACTGGTTTATTTCTGACATGGAGTGAAGGGAAATTCCCAGAATGTCAGAACTGCAGTGGAAGGTGCTATATCAATTACAGCAGCAATCAAGTCTTGGAGTGTTATCCTCCCATTCCCAGACTAAAACACAAAGGTCTATCAACTAGTTTTATTGTCATGGTAGTGAGTGTATGTGGGTTTGTTGTCTTGTCAACTACGGCGGCATCCATCTTTTACCATTTAAAGCAATCAATAAAGAGGAAAGAGGAAAAGGAGAATCAAAGAAAGATTGAAAAGTTCCTAGATGATCACAAGTCCCATGTACCAACAAGATACTCCTATGCTGATATAAAGAAGATGACAAATGGATTCAAGAAGAAGTTGGGTGAAGGAGGTTTTGGAAGTGTTTTCAGTGGAAAGCTTCCCACTAATGGAGTTCCGGTAGCCATAAAAGTCCTCAAGGATTCTGAAGGAAATGGAGATGATTTTGTTAATGAAGTGGGAACCATTGGTAGGATTCACCATGTAAACGTGGTCCGCCTACTTGGGTTTTCCGCTGAAGGAGGCAAACGTGCTCTTATTTATGAGCTCATGCCAAACAGTTCTCTGGGGAAGTTTATCACATCTAGAGGATCAAATAAGAGCAGCAGTTCATTTAACTGGGAGAAACTTTGCAACATCATCACAGGTATAGCAAAGGGAATCGAGTACCTTCACCAAGGGTGTGACCAGAGGATTCTCCACTTTGATATCAAGCCTCACAATATCTTGTTAGACCATGCCTTTAATCCGAAGATTTCTGATTTTGGTATGGCTAAACTCTGTTCCAAGGAAGATAGTGTTATATCTATGACTGCTGCCAGAGGCACTATGGGCTACATTGCGCCTGAAGTATTTAATGGCAACTTTGGAACTGTCTCCTACAAATCGGATGTGTATAGTTTCGGGATGCTAGTTCTTGAAGTTATTGGAGCCATGAAGGAAACTGGTCTTACATCTAGCGACAATGAGGTGTATTTTCCGGAATTGATTTATAAGTGTCTGATCCAAGGAGAAGAGTTGGATTTGGAGCTAGCTGATGATGGAGATGCTCAGATTGCTAAGAGATTAGCAATTGTGGCACTTTGGTGCATCCAGTGGTACCCGGTGAGTCGCCCTTCCATGAAAGCAGTTGTTAGAATGCTGGAAGGAACCTTTGAAAGCTTGATCATGCCACCCAATCCGTTTGCTTCCACAACTCAGATGGAACCAACAACAACCCCATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

636

Amino Acids

71.24

Weight (kDa)

6.24

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 102 1.5e-13 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 337 - 608 6.5e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 338 - 606 1.5e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1674
Acc65I GGTACC 1 cut(s) 1779
AccB1I GGYRCC 1 cut(s) 1779
AccI GTMKAC 1 cut(s) 579
AccII CGCG 1 cut(s) 503
AccIII TCCGGA 1 cut(s) 1660
AciI CCGC 4 cut(s) 551, 860, 1177, 1194
AclWI GGATC 2 cut(s) 1279, 1678
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 2 cut(s) 454, 692
AcuI CTGAAG 5 cut(s) 41, 402, 1128, 1218, 1548
AcyI GRCGYC 1 cut(s) 328
AfaI GTAC 5 cut(s) 442, 497, 963, 1349, 1781
AfiI CCNNNNNNNGG 4 cut(s) 489, 629, 1070, 1152
AgsI TTSAA 7 cut(s) 68, 365, 620, 932, 1012, 1601, 1838
AhlI ACTAGT 1 cut(s) 803
AjnI CCWGG 1 cut(s) 523
AjuI GAANNNNNNNTTGG 2 cut(s) 1020, 1052
AleI CACNNNNGTG 1 cut(s) 1765
AluBI AGCT 7 cut(s) 19, 235, 1060, 1228, 1711, 1715, 1842
AluI AGCT 7 cut(s) 19, 235, 1060, 1228, 1711, 1715, 1842
Alw21I GWGCWC 2 cut(s) 1216, 1230
Alw26I GTCTC 4 cut(s) 319, 473, 518, 1561
AlwI GGATC 2 cut(s) 1279, 1678
Ama87I CYCGRG 1 cut(s) 322
Aor13HI TCCGGA 1 cut(s) 1660
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 4 cut(s) 204, 741, 1284, 1496
ApoI RAATTY 2 cut(s) 454, 692
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 1 cut(s) 606
Asp718I GGTACC 1 cut(s) 1779
AspLEI GCGC 1 cut(s) 1525
AspS9I GGNCC 2 cut(s) 128, 1174
AsuC2I CCSGG 1 cut(s) 1784
AsuHPI GGTGA 7 cut(s) 205, 460, 644, 1037, 1151, 1346, 1798
AsuNHI GCTAGC 1 cut(s) 1711
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 2 cut(s) 128, 1174
BalI TGGCCA 1 cut(s) 180
BanI GGYRCC 1 cut(s) 1779
BanII GRGCYC 1 cut(s) 1230
Bbv12I GWGCWC 2 cut(s) 1216, 1230
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 4 cut(s) 216, 753, 1296, 1483
BccI CCATC 7 cut(s) 119, 189, 305, 875, 1715, 1876, 1909
BceAI ACGGC 2 cut(s) 462, 873
BciT130I CCWGG 1 cut(s) 525
BclI TGATCA 3 cut(s) 91, 946, 1845
BcnI CCSGG 1 cut(s) 1784
BcoDI GTCTC 4 cut(s) 319, 473, 518, 1561
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 7 cut(s) 656, 804, 941, 1266, 1592, 1638, 1712
BfmI CTRYAG 2 cut(s) 202, 712
BisI GCNGC 5 cut(s) 205, 742, 861, 1285, 1497
BlsI GCNGC 5 cut(s) 206, 743, 862, 1286, 1498
Bme1390I CCNGG 2 cut(s) 525, 1784
Bme18I GGWCC 2 cut(s) 128, 1174
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 128, 1174
BmiI GGNNCC 6 cut(s) 130, 1144, 1612, 1781, 1831, 1887
BmrFI CCNGG 2 cut(s) 525, 1784
BmrI ACTGGG 2 cut(s) 98, 1309
BmsI GCATC 5 cut(s) 538, 872, 1578, 1717, 1779
BmtI GCTAGC 1 cut(s) 1715
BmuI ACTGGG 2 cut(s) 98, 1309
Bpu10I CCTNAGC 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 1 cut(s) 1784
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 328
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 5 cut(s) 523, 524, 1357, 1467, 1687
BsaWI WCCGGW 2 cut(s) 1078, 1660
BsaXI ACNNNNNCTCC 2 cut(s) 1065, 1095
Bsc4I CCNNNNNNNGG 4 cut(s) 489, 629, 1070, 1152
Bse118I RCCGGY 1 cut(s) 574
Bse1I ACTGG 7 cut(s) 104, 139, 494, 667, 1304, 1630, 1774
Bse3DI GCAATG 1 cut(s) 1518
BseAI TCCGGA 1 cut(s) 1660
BseBI CCWGG 1 cut(s) 525
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 5 cut(s) 523, 524, 1357, 1467, 1687
BseGI GGATG 6 cut(s) 181, 316, 863, 1576, 1593, 1770
BseLI CCNNNNNNNGG 4 cut(s) 489, 629, 1070, 1152
BseMI GCAATG 1 cut(s) 1518
BseMII CTCAG 5 cut(s) 29, 468, 529, 1745, 1892
BseNI ACTGG 7 cut(s) 104, 139, 494, 667, 1304, 1630, 1774
BseRI GAGGAG 2 cut(s) 340, 343
BseXI GCAGC 4 cut(s) 216, 753, 1296, 1483
Bsh1236I CGCG 1 cut(s) 503
BshFI GGCC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 1779
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 1216, 1230
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 4 cut(s) 575, 1079, 1661, 1784
BslFI GGGAC 4 cut(s) 114, 297, 506, 940
BslI CCNNNNNNNGG 4 cut(s) 489, 629, 1070, 1152
BsmAI GTCTC 4 cut(s) 319, 473, 518, 1561
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 4 cut(s) 114, 297, 506, 940
BsmI GAATGC 1 cut(s) 1824
BsnI GGCC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 1216, 1230
Bsp13I TCCGGA 1 cut(s) 1660
Bsp143I GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
Bsp68I TCGCGA 1 cut(s) 503
BspACI CCGC 4 cut(s) 551, 860, 1177, 1194
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 5 cut(s) 28, 469, 528, 1744, 1891
BspDI ATCGAT 1 cut(s) 273
BspEI TCCGGA 1 cut(s) 1660
BspFNI CGCG 1 cut(s) 503
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 6 cut(s) 130, 1144, 1612, 1781, 1831, 1887
BspMAI CTGCAG 2 cut(s) 206, 716
BspOI GCTAGC 1 cut(s) 1715
BspPI GGATC 2 cut(s) 1279, 1678
BspT107I GGYRCC 1 cut(s) 1779
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 1518
BsrFI RCCGGY 1 cut(s) 574
BsrI ACTGG 7 cut(s) 104, 139, 494, 667, 1304, 1630, 1774
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 5 cut(s) 523, 524, 1357, 1467, 1687
BssMI GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
BssNI GRCGYC 1 cut(s) 328
BssT1I CCWWGG 3 cut(s) 1357, 1467, 1687
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 5 cut(s) 64, 176, 291, 1241, 1555
Bst6I CTCTTC 1 cut(s) 1689
BstACI GRCGYC 1 cut(s) 328
BstC8I GCNNGC 2 cut(s) 586, 1713
BstDEI CTNAG 6 cut(s) 15, 477, 515, 1731, 1740, 1878
BstF5I GGATG 6 cut(s) 181, 316, 863, 1576, 1593, 1770
BstFNI CGCG 1 cut(s) 503
BstHHI GCGC 1 cut(s) 1525
BstKTI GATC 6 cut(s) 94, 507, 949, 1274, 1686, 1848
BstMAI GTCTC 4 cut(s) 319, 473, 518, 1561
BstMBI GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
BstMWI GCNNNNNNNGC 4 cut(s) 37, 1512, 1736, 1757
BstNI CCWGG 1 cut(s) 525
BstSCI CCNGG 2 cut(s) 523, 1782
BstSFI CTRYAG 2 cut(s) 202, 712
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 4 cut(s) 216, 753, 1296, 1483
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 1 cut(s) 180
BsuTUI ATCGAT 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 484
BtsCI GGATG 6 cut(s) 181, 316, 863, 1576, 1593, 1770
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 6 cut(s) 172, 523, 660, 721, 1057, 1781
BtuMI TCGCGA 1 cut(s) 503
Cac8I GCNNGC 2 cut(s) 586, 1713
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 1525
Cfr10I RCCGGY 1 cut(s) 574
Cfr13I GGNCC 2 cut(s) 128, 1174
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 2 cut(s) 336, 590
Csp6I GTAC 5 cut(s) 441, 496, 962, 1348, 1780
CviQI GTAC 5 cut(s) 441, 496, 962, 1348, 1780
DdeI CTNAG 6 cut(s) 15, 477, 515, 1731, 1740, 1878
DpnI GATC 6 cut(s) 93, 506, 948, 1273, 1685, 1847
DpnII GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
DraI TTTAAA 1 cut(s) 882
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 1689
EarI CTCTTC 1 cut(s) 1689
EciI GGCGGA 1 cut(s) 1166
Ecl136II GAGCTC 1 cut(s) 1228
Eco130I CCWWGG 3 cut(s) 1357, 1467, 1687
Eco24I GRGCYC 1 cut(s) 1230
Eco31I GGTCTC 1 cut(s) 473
Eco32I GATATC 2 cut(s) 7, 1390
Eco47I GGWCC 2 cut(s) 128, 1174
Eco53kI GAGCTC 1 cut(s) 1228
Eco57I CTGAAG 5 cut(s) 41, 402, 1128, 1218, 1548
Eco88I CYCGRG 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 1228
EcoRII CCWGG 1 cut(s) 523
EcoRV GATATC 2 cut(s) 7, 1390
EcoT14I CCWWGG 3 cut(s) 1357, 1467, 1687
EcoT38I GRGCYC 1 cut(s) 1230
ErhI CCWWGG 3 cut(s) 1357, 1467, 1687
Esp3I CGTCTC 1 cut(s) 319
FaqI GGGAC 4 cut(s) 114, 297, 506, 940
FbaI TGATCA 3 cut(s) 91, 946, 1845
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 5 cut(s) 205, 742, 861, 1285, 1497
FokI GGATG 6 cut(s) 168, 323, 850, 1583, 1600, 1757
FriOI GRGCYC 1 cut(s) 1230
Fsp4HI GCNGC 5 cut(s) 205, 742, 861, 1285, 1497
FspBI CTAG 7 cut(s) 656, 804, 941, 1266, 1592, 1638, 1712
GlaI GCGC 1 cut(s) 1524
GluI GCNGC 5 cut(s) 205, 742, 861, 1285, 1497
HaeIII GGCC 1 cut(s) 180
HapII CCGG 4 cut(s) 575, 1079, 1661, 1784
HgaI GACGC 2 cut(s) 336, 590
HhaI GCGC 1 cut(s) 1525
Hin1I GRCGYC 1 cut(s) 328
Hin6I GCGC 1 cut(s) 1523
HinP1I GCGC 1 cut(s) 1523
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HindIII AAGCTT 3 cut(s) 233, 1058, 1840
HpaII CCGG 4 cut(s) 575, 1079, 1661, 1784
HphI GGTGA 7 cut(s) 205, 460, 644, 1037, 1151, 1346, 1798
Hpy166II GTNNAC 5 cut(s) 155, 580, 653, 852, 1168
Hpy8I GTNNAC 5 cut(s) 155, 580, 653, 852, 1168
Hpy99I CGWCG 1 cut(s) 448
HpyCH4III ACNGT 5 cut(s) 64, 176, 291, 1241, 1555
HpyCH4IV ACGT 4 cut(s) 413, 428, 1170, 1210
HpyCH4V TGCA 6 cut(s) 204, 301, 396, 714, 1314, 1770
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 1512, 1736, 1757
HpyF3I CTNAG 6 cut(s) 15, 477, 515, 1731, 1740, 1878
HpySE526I ACGT 4 cut(s) 413, 428, 1170, 1210
Hsp92I GRCGYC 1 cut(s) 328
HspAI GCGC 1 cut(s) 1523
Kpn2I TCCGGA 1 cut(s) 1660
KpnI GGTACC 1 cut(s) 1783
Ksp22I TGATCA 3 cut(s) 91, 946, 1845
Kzo9I GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
LmnI GCTCC 2 cut(s) 1610, 1708
Lsp1109I GCAGC 4 cut(s) 216, 753, 1296, 1483
LweI GCATC 5 cut(s) 538, 872, 1578, 1717, 1779
MaeI CTAG 7 cut(s) 656, 804, 941, 1266, 1592, 1638, 1712
MaeII ACGT 4 cut(s) 413, 428, 1170, 1210
MaeIII GTNAC 3 cut(s) 398, 566, 1364
MalI GATC 6 cut(s) 93, 506, 948, 1273, 1685, 1847
MboI GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
MboII GAAGA 7 cut(s) 395, 602, 1006, 1027, 1444, 1484, 1706
MfeI CAATTG 1 cut(s) 1752
MhlI GDGCHC 2 cut(s) 1216, 1230
MlsI TGGCCA 1 cut(s) 180
MluCI AATT 8 cut(s) 72, 337, 436, 454, 692, 733, 1664, 1752
MluNI TGGCCA 1 cut(s) 180
MlyI GAGTC 1 cut(s) 1798
MmeI TCCRAC 1 cut(s) 1680
Mox20I TGGCCA 1 cut(s) 180
MroI TCCGGA 1 cut(s) 1660
MroXI GAANNNNTTC 1 cut(s) 606
MscI TGGCCA 1 cut(s) 180
MseI TTAA 8 cut(s) 336, 434, 881, 1131, 1296, 1425, 1536, 1909
MslI CAYNNNNRTG 3 cut(s) 224, 821, 1765
Msp20I TGGCCA 1 cut(s) 180
MspA1I CMGCKG 2 cut(s) 19, 1196
MspI CCGG 4 cut(s) 575, 1079, 1661, 1784
MspR9I CCNGG 2 cut(s) 525, 1784
MunI CAATTG 1 cut(s) 1752
Mva1269I GAATGC 1 cut(s) 1824
MvaI CCWGG 1 cut(s) 525
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 4 cut(s) 37, 1512, 1736, 1757
NciI CCSGG 1 cut(s) 1784
NdeII GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
NheI GCTAGC 1 cut(s) 1711
NlaIV GGNNCC 6 cut(s) 130, 1144, 1612, 1781, 1831, 1887
NmuCI GTSAC 2 cut(s) 566, 1364
NruI TCGCGA 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 1765
PagI TCATGA 1 cut(s) 94
PasI CCCWGGG 1 cut(s) 524
PctI GAATGC 1 cut(s) 1824
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 9 cut(s) 210, 252, 386, 916, 1008, 1103, 1156, 1341, 1375
PkrI GCNGC 5 cut(s) 206, 743, 862, 1286, 1498
PleI GAGTC 1 cut(s) 1797
PpsI GAGTC 1 cut(s) 1797
PsiI TTATAA 1 cut(s) 1674
Psp124BI GAGCTC 1 cut(s) 1230
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 6 cut(s) 130, 1144, 1612, 1781, 1831, 1887
PspPI GGNCC 2 cut(s) 128, 1174
PstI CTGCAG 2 cut(s) 206, 716
PvuII CAGCTG 1 cut(s) 19
RruI TCGCGA 1 cut(s) 503
RsaI GTAC 5 cut(s) 442, 497, 963, 1349, 1781
RsaNI GTAC 5 cut(s) 441, 496, 962, 1348, 1780
RseI CAYNNNNRTG 3 cut(s) 224, 821, 1765
SacI GAGCTC 1 cut(s) 1230
SaqAI TTAA 8 cut(s) 336, 434, 881, 1131, 1296, 1425, 1536, 1909
SatI GCNGC 5 cut(s) 205, 742, 861, 1285, 1497
Sau3AI GATC 6 cut(s) 91, 504, 946, 1271, 1683, 1845
Sau96I GGNCC 2 cut(s) 128, 1174
SchI GAGTC 1 cut(s) 1798
ScrFI CCNGG 2 cut(s) 525, 1784
SduI GDGCHC 2 cut(s) 1216, 1230
SfaNI GCATC 5 cut(s) 538, 872, 1578, 1717, 1779
SfcI CTRYAG 2 cut(s) 202, 712
SinI GGWCC 2 cut(s) 128, 1174
SmiMI CAYNNNNRTG 3 cut(s) 224, 821, 1765
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
SpeI ACTAGT 1 cut(s) 803
Sse9I AATT 8 cut(s) 72, 337, 436, 454, 692, 733, 1664, 1752
SsiI CCGC 4 cut(s) 551, 860, 1177, 1194
SspMI CTAG 7 cut(s) 656, 804, 941, 1266, 1592, 1638, 1712
SstI GAGCTC 1 cut(s) 1230
StyD4I CCNGG 2 cut(s) 523, 1782
StyI CCWWGG 3 cut(s) 1357, 1467, 1687
TaaI ACNGT 5 cut(s) 64, 176, 291, 1241, 1555
TaiI ACGT 4 cut(s) 416, 431, 1173, 1213
TaqI TCGA 2 cut(s) 273, 1344
TasI AATT 8 cut(s) 72, 337, 436, 454, 692, 733, 1664, 1752
TatI WGTACW 1 cut(s) 495
TauI GCSGC 1 cut(s) 863
TfiI GAWTC 9 cut(s) 210, 252, 386, 916, 1008, 1103, 1156, 1341, 1375
Tru1I TTAA 8 cut(s) 336, 434, 881, 1131, 1296, 1425, 1536, 1909
Tru9I TTAA 8 cut(s) 336, 434, 881, 1131, 1296, 1425, 1536, 1909
TscAI CASTG 6 cut(s) 179, 523, 667, 721, 1057, 1781
TseFI GTSAC 2 cut(s) 566, 1364
TseI GCWGC 4 cut(s) 204, 741, 1284, 1496
Tsp45I GTSAC 2 cut(s) 566, 1364
TspDTI ATGAA 5 cut(s) 245, 1149, 1281, 1631, 1817
TspGWI ACGGA 3 cut(s) 140, 379, 1851
TspRI CASTG 6 cut(s) 179, 523, 667, 721, 1057, 1781
VpaK11BI GGWCC 2 cut(s) 128, 1174
XapI RAATTY 2 cut(s) 454, 692
XbaI TCTAGA 1 cut(s) 1265
XcmI CCANNNNNNNNNTGG 2 cut(s) 1507, 1879
XmiI GTMKAC 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 7 cut(s) 656, 804, 941, 1266, 1592, 1638, 1712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.