Rw7G021310

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000953
Physical Location & Seq
Reverse (-)
24660004 .. 24662059
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021310.1

Sequence Viewer

Length: 1851 bp
ATGGGAATTCTGCAGCTGCTGCTACCTTTGTTTTTCTGCTCCATTGTTCTTGTACCGAGCAAAGCAGCTGATCATGACTGTCCAGTTTCAAAGTGTAGTCCTGATGGTCCCCTTATCAAGTTTCCATTCCGTCTAAAGCATCAACCCCTTCACTGTGGCAATCCAGAGTTTGAGGTTTCCTGCAGCAGCTTCAGCAATACGACCATGATTCAGCTCTCCTCATCTTCAGGACTCTTCACCATCCAAACAATTGATTATGAAAACCAATACTTCGACGTCTACAACAGAGATGGCTGCCTTCCTAGACGGCTTGTAAACTTTACCATCTCCAGCAGCTCACTCTTTCAACCAGCACGTTTATTTGGGAATGACTACTGCATCTTTTCTGGGAAATATTTTCCCGACTTAAAATTACTGAATTGTTCCACACCAGAAAACTTTAGCACAGGGAGTTTCAACAAGAAGTACATTCCCATCACATGTCTTAGTATACCAGGGCATCAGGTTGTGGCTGCTCTACCCTCAACTTCTTTATCTGACTTGCCGGTGCCAAGATGCCGTAGTTTGATTGGACCAATCTATCTTCCACTACGAAGAGTGGATGGTAATCCGGACAACTGTGTACCAGATAATGTATTAATTCTGAAATGGGACTTGGGCGATTTCCCAGGATGTCAAAACTGTAAAGCGGGAAGGTGCCAATTCAATTATAACAGCAATCAATTGGAGTGTCATCCTGAACTCAGCCCTCCACCTCCACCTCCATCTCCACCTCCACCTTCACCTGGTGAATCTAACTCAAAAAGGCCCAGGTATATCATAGGAGTGAGCATAGCTTGTTTTGTTCTCATCTCGGTAGCATCGGTAGCAATTTTCTTCCATGTCACACAAACAAAAAGGATAGAGGAAAAGGAGAATCAAATGAAAATTGAAAGGTTCCTAAATGATCACAAGTCTCATGTACCCACTAGATACTCCTGTGCTAGTGAAGGAGGTTTTGGAAGTGTTTTCTTGGGAAAGCTACCCAACGGTGTTCAAGTCGCCGTAAAAGTCCTCAATGGTTCTAAAAGAAGTGGAGAAGATTTTATCAATGAAGTGGGAACCATTGGTAGAATTCACCATGTTAATGTGGTTCGCTTACTTGGGTTCTCTGCTGAAGGATGCAACCGTGCTCTCATTTATGAGTTCATGCCAAACAAGTCCTTAGAAAAGTTCATCACATCTAGAGAATCTAATAATAATGACACTACATTTGATTGGAAGAAACTTCAGCACATTATCACGGGTATAGGAAAGGGGATTGAATACCTTCACCAAGGGTGTGATCAGAGGATCCTCCACTTTGATATCAAGCCTCAGAATATCTTGCTAGACCATGATTTCAACCCTAAGATCTCTGATTTTGGTCTTGCAAAACTCTGTTCCAAGGGAGAAAGCATAATATCAATGACTGCTGCTAGAGGCACAGTAGGCTACATCGCACCCGAAGTGTTGATTAGGAACTTCACAAACGTGTCCTACAAATCAGATGTATATAGTTTTGGGATGCTAGTGCTTGAAATGATAGGAGCTAGAAGGGAACCTCCTCTTGGAACTATTAACAGTGAGGTGTATTTTCCTGAATGGATTTATAAGCGTCTAATTCAGGGAGGAACATTGGATTTGAAGCTAGATACTGATGAAGATGCTCATGTTGCGAAGAAACTAGTGATTGTTGCAATATGGTGCATCCAGTGGCACCCGGTGAATCGCCCATCCATGAAATCTGTTGTTAGAATGCTAGAAGGAGATTCTGAAAGCTTGATCATGCCACCAAATCCATTCACCTTCACAAGTACTCAGACAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

616

Amino Acids

68.7

Weight (kDa)

8.13

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 94 3.2e-14 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 330 - 592 2.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 330 - 594 5e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 711, 1632
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 3 cut(s) 547, 696, 1737
AccI GTMKAC 2 cut(s) 279, 490
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 1 cut(s) 689
AclWI GGATC 2 cut(s) 1327, 1340
AcsI RAATTY 2 cut(s) 6, 1113
AcuI CTGAAG 4 cut(s) 175, 210, 1176, 1253
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 2 cut(s) 788, 1744
AfaI GTAC 5 cut(s) 54, 467, 624, 963, 1837
AfiI CCNNNNNNNGG 2 cut(s) 785, 1589
AflIII ACRYGT 2 cut(s) 479, 1512
AhlI ACTAGT 1 cut(s) 1705
AjnI CCWGG 4 cut(s) 493, 667, 784, 809
AjuI GAANNNNNNNTTGG 6 cut(s) 638, 670, 981, 1013, 1572, 1604
AleI CACNNNNGTG 1 cut(s) 1511
Alw21I GWGCWC 1 cut(s) 1174
Alw26I GTCTC 1 cut(s) 960
AlwI GGATC 2 cut(s) 1327, 1340
AlwNI CAGNNNCTG 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 1 cut(s) 806
ApoI RAATTY 2 cut(s) 6, 1113
ArsI GACNNNNNNTTYG 2 cut(s) 1235, 1267
AseI ATTAAT 1 cut(s) 638
Asp700I GAANNNNTTC 1 cut(s) 1308
AspS9I GGNCC 3 cut(s) 107, 572, 807
AsuC2I CCSGG 1 cut(s) 1742
AsuHPI GGTGA 7 cut(s) 229, 774, 800, 1109, 1304, 1756, 1816
AvaII GGWCC 2 cut(s) 107, 572
BaeI ACNNNNGTAYC 2 cut(s) 964, 997
BamHI GGATCC 1 cut(s) 1332
BanI GGYRCC 3 cut(s) 547, 696, 1737
Bbv12I GWGCWC 1 cut(s) 1174
BccI CCATC 8 cut(s) 98, 248, 284, 332, 482, 596, 772, 1762
BceAI ACGGC 3 cut(s) 323, 543, 1028
BciT130I CCWGG 4 cut(s) 495, 669, 786, 811
BclI TGATCA 4 cut(s) 70, 946, 1324, 1803
BcnI CCSGG 1 cut(s) 1742
BcoDI GTCTC 1 cut(s) 960
BcuI ACTAGT 1 cut(s) 1705
BfmI CTRYAG 2 cut(s) 11, 181
BglII AGATCT 1 cut(s) 1392
BmcAI AGTACT 1 cut(s) 1837
Bme1390I CCNGG 5 cut(s) 495, 669, 786, 811, 1742
Bme18I GGWCC 2 cut(s) 107, 572
BmgT120I GGNCC 3 cut(s) 107, 572, 807
BmiI GGNNCC 8 cut(s) 109, 549, 698, 938, 1102, 1334, 1581, 1739
BmrFI CCNGG 5 cut(s) 495, 669, 786, 811, 1742
BmsI GCATC 9 cut(s) 148, 387, 508, 545, 869, 1151, 1536, 1675, 1737
BpmI CTGGAG 1 cut(s) 313
BpuMI CCSGG 1 cut(s) 1742
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 5 cut(s) 494, 667, 809, 1315, 1425
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 2 cut(s) 785, 1589
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 2 cut(s) 83, 1732
Bse8I GATNNNNATC 1 cut(s) 606
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 4 cut(s) 495, 669, 786, 811
BseDI CCNNGG 5 cut(s) 494, 667, 809, 1315, 1425
BseGI GGATG 8 cut(s) 240, 607, 677, 733, 1166, 1551, 1728, 1754
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 2 cut(s) 785, 1589
BseMII CTCAG 2 cut(s) 757, 1370
BseNI ACTGG 2 cut(s) 83, 1732
BseRI GAGGAG 2 cut(s) 208, 1575
BshFI GGCC 1 cut(s) 808
BshNI GGYRCC 3 cut(s) 547, 696, 1737
BsiHKAI GWGCWC 1 cut(s) 1174
BsiSI CCGG 3 cut(s) 545, 611, 1742
BslFI GGGAC 2 cut(s) 93, 665
BslI CCNNNNNNNGG 2 cut(s) 785, 1589
BsmAI GTCTC 1 cut(s) 960
BsmFI GGGAC 2 cut(s) 93, 665
BsmI GAATGC 1 cut(s) 1782
BsnI GGCC 1 cut(s) 808
Bsp1286I GDGCHC 1 cut(s) 1174
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
BspACI CCGC 1 cut(s) 689
BspANI GGCC 1 cut(s) 808
BspCNI CTCAG 2 cut(s) 756, 1369
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 8 cut(s) 109, 549, 698, 938, 1102, 1334, 1581, 1739
BspMAI CTGCAG 2 cut(s) 15, 185
BspPI GGATC 2 cut(s) 1327, 1340
BspT107I GGYRCC 3 cut(s) 547, 696, 1737
BsrFI RCCGGY 1 cut(s) 544
BsrI ACTGG 2 cut(s) 83, 1732
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 5 cut(s) 494, 667, 809, 1315, 1425
BssMI GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
BssNAI GTATAC 1 cut(s) 491
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 1315, 1425
Bst1107I GTATAC 1 cut(s) 491
Bst2UI CCWGG 4 cut(s) 495, 669, 786, 811
Bst4CI ACNGT 8 cut(s) 80, 155, 620, 683, 1031, 1169, 1468, 1604
Bst6I CTCTTC 2 cut(s) 239, 589
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 19
BstDEI CTNAG 6 cut(s) 485, 743, 1204, 1356, 1389, 1839
BstF5I GGATG 8 cut(s) 240, 607, 677, 733, 1166, 1551, 1728, 1754
BstKTI GATC 6 cut(s) 73, 949, 1327, 1335, 1395, 1806
BstMAI GTCTC 1 cut(s) 960
BstMBI GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
BstMWI GCNNNNNNNGC 5 cut(s) 19, 192, 866, 1470, 1694
BstNI CCWGG 4 cut(s) 495, 669, 786, 811
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 5 cut(s) 493, 667, 784, 809, 1740
BstSFI CTRYAG 2 cut(s) 11, 181
BstX2I RGATCY 2 cut(s) 1332, 1392
BstYI RGATCY 2 cut(s) 1332, 1392
BstZ17I GTATAC 1 cut(s) 491
BsuRI GGCC 1 cut(s) 808
BtgZI GCGATG 1 cut(s) 1462
BtsCI GGATG 8 cut(s) 240, 607, 677, 733, 1166, 1551, 1728, 1754
BtsIMutI CAGTG 3 cut(s) 151, 1609, 1739
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 3 cut(s) 107, 572, 807
CseI GACGC 1 cut(s) 1625
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 5 cut(s) 53, 466, 623, 962, 1836
CviQI GTAC 5 cut(s) 53, 466, 623, 962, 1836
DdeI CTNAG 6 cut(s) 485, 743, 1204, 1356, 1389, 1839
DpnI GATC 6 cut(s) 72, 948, 1326, 1334, 1394, 1805
DpnII GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
DraIII CACNNNGTG 2 cut(s) 788, 1744
Eam1104I CTCTTC 2 cut(s) 239, 589
EarI CTCTTC 2 cut(s) 239, 589
Eco130I CCWWGG 2 cut(s) 1315, 1425
Eco32I GATATC 1 cut(s) 1348
Eco47I GGWCC 2 cut(s) 107, 572
Eco57I CTGAAG 4 cut(s) 175, 210, 1176, 1253
EcoRI GAATTC 2 cut(s) 6, 1113
EcoRII CCWGG 4 cut(s) 493, 667, 784, 809
EcoRV GATATC 1 cut(s) 1348
EcoT14I CCWWGG 2 cut(s) 1315, 1425
ErhI CCWWGG 2 cut(s) 1315, 1425
FaqI GGGAC 2 cut(s) 93, 665
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 4 cut(s) 70, 946, 1324, 1803
FblI GTMKAC 2 cut(s) 279, 490
FokI GGATG 8 cut(s) 227, 614, 684, 720, 1173, 1558, 1715, 1741
GsuI CTGGAG 1 cut(s) 313
HaeIII GGCC 1 cut(s) 808
HapII CCGG 3 cut(s) 545, 611, 1742
HgaI GACGC 1 cut(s) 1625
Hin1I GRCGYC 1 cut(s) 276
HindIII AAGCTT 1 cut(s) 1798
HinfI GANTC 7 cut(s) 208, 231, 791, 916, 1229, 1747, 1790
HpaII CCGG 3 cut(s) 545, 611, 1742
HphI GGTGA 7 cut(s) 229, 774, 800, 1109, 1304, 1756, 1816
Hpy166II GTNNAC 4 cut(s) 280, 316, 491, 623
Hpy188I TCNGA 8 cut(s) 538, 645, 1329, 1359, 1399, 1528, 1795, 1842
Hpy188III TCNNGA 9 cut(s) 74, 101, 164, 228, 401, 611, 737, 1224, 1619
Hpy8I GTNNAC 4 cut(s) 280, 316, 491, 623
Hpy99I CGWCG 1 cut(s) 278
HpyCH4III ACNGT 8 cut(s) 80, 155, 620, 683, 1031, 1169, 1468, 1604
HpyCH4IV ACGT 3 cut(s) 276, 355, 1512
HpyCH4V TGCA 7 cut(s) 13, 183, 378, 1164, 1412, 1718, 1728
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 192, 866, 1470, 1694
HpyF3I CTNAG 6 cut(s) 485, 743, 1204, 1356, 1389, 1839
HpySE526I ACGT 3 cut(s) 276, 355, 1512
Hsp92I GRCGYC 1 cut(s) 276
Kpn2I TCCGGA 1 cut(s) 610
Ksp22I TGATCA 4 cut(s) 70, 946, 1324, 1803
Kzo9I GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
LmnI GCTCC 2 cut(s) 44, 1568
LweI GCATC 9 cut(s) 148, 387, 508, 545, 869, 1151, 1536, 1675, 1737
MabI ACCWGGT 1 cut(s) 784
MaeII ACGT 3 cut(s) 276, 355, 1512
MaeIII GTNAC 1 cut(s) 883
MalI GATC 6 cut(s) 72, 948, 1326, 1334, 1394, 1805
MboI GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
MboII GAAGA 9 cut(s) 216, 226, 575, 606, 868, 1091, 1273, 1694, 1711
MfeI CAATTG 2 cut(s) 249, 722
MflI RGATCY 2 cut(s) 1332, 1392
MhlI GDGCHC 1 cut(s) 1174
MlyI GAGTC 1 cut(s) 225
MroI TCCGGA 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 1308
MseI TTAA 4 cut(s) 407, 638, 1125, 1599
MslI CAYNNNNRTG 3 cut(s) 824, 1125, 1511
MspA1I CMGCKG 2 cut(s) 16, 68
MspI CCGG 3 cut(s) 545, 611, 1742
MspR9I CCNGG 5 cut(s) 495, 669, 786, 811, 1742
MunI CAATTG 2 cut(s) 249, 722
Mva1269I GAATGC 1 cut(s) 1782
MvaI CCWGG 4 cut(s) 495, 669, 786, 811
MwoI GCNNNNNNNGC 5 cut(s) 19, 192, 866, 1470, 1694
NciI CCSGG 1 cut(s) 1742
NdeII GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
NlaIV GGNNCC 8 cut(s) 109, 549, 698, 938, 1102, 1334, 1581, 1739
NmuCI GTSAC 1 cut(s) 883
NspI RCATGY 1 cut(s) 483
OliI CACNNNNGTG 1 cut(s) 1511
PagI TCATGA 1 cut(s) 73
PciI ACATGT 1 cut(s) 479
PctI GAATGC 1 cut(s) 1782
PdmI GAANNNNTTC 1 cut(s) 1308
PfeI GAWTC 6 cut(s) 208, 791, 916, 1229, 1747, 1790
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PscI ACATGT 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 638
PsiI TTATAA 2 cut(s) 711, 1632
Psp6I CCWGG 4 cut(s) 493, 667, 784, 809
PspGI CCWGG 4 cut(s) 493, 667, 784, 809
PspN4I GGNNCC 8 cut(s) 109, 549, 698, 938, 1102, 1334, 1581, 1739
PspPI GGNCC 3 cut(s) 107, 572, 807
PstI CTGCAG 2 cut(s) 15, 185
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 2 cut(s) 1332, 1392
PvuII CAGCTG 2 cut(s) 16, 68
RsaI GTAC 5 cut(s) 54, 467, 624, 963, 1837
RsaNI GTAC 5 cut(s) 53, 466, 623, 962, 1836
RseI CAYNNNNRTG 3 cut(s) 824, 1125, 1511
SaqAI TTAA 4 cut(s) 407, 638, 1125, 1599
Sau3AI GATC 6 cut(s) 70, 946, 1324, 1332, 1392, 1803
Sau96I GGNCC 3 cut(s) 107, 572, 807
ScaI AGTACT 1 cut(s) 1837
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 5 cut(s) 495, 669, 786, 811, 1742
SduI GDGCHC 1 cut(s) 1174
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 9 cut(s) 148, 387, 508, 545, 869, 1151, 1536, 1675, 1737
SfcI CTRYAG 2 cut(s) 11, 181
SinI GGWCC 2 cut(s) 107, 572
SmiMI CAYNNNNRTG 3 cut(s) 824, 1125, 1511
SpeI ACTAGT 1 cut(s) 1705
SsiI CCGC 1 cut(s) 689
SspI AATATT 1 cut(s) 395
StyD4I CCNGG 5 cut(s) 493, 667, 784, 809, 1740
StyI CCWWGG 2 cut(s) 1315, 1425
TaaI ACNGT 8 cut(s) 80, 155, 620, 683, 1031, 1169, 1468, 1604
TaiI ACGT 3 cut(s) 279, 358, 1515
TaqI TCGA 1 cut(s) 273
TatI WGTACW 2 cut(s) 465, 1835
TfiI GAWTC 6 cut(s) 208, 791, 916, 1229, 1747, 1790
Tru1I TTAA 4 cut(s) 407, 638, 1125, 1599
Tru9I TTAA 4 cut(s) 407, 638, 1125, 1599
TscAI CASTG 3 cut(s) 158, 1609, 1739
TseFI GTSAC 1 cut(s) 883
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 7 cut(s) 273, 938, 1107, 1177, 1204, 1695, 1775
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 3 cut(s) 158, 1609, 1739
VpaK11BI GGWCC 2 cut(s) 107, 572
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 2 cut(s) 6, 1113
XbaI TCTAGA 1 cut(s) 1223
XceI RCATGY 1 cut(s) 483
XmiI GTMKAC 2 cut(s) 279, 490
XmnI GAANNNNTTC 1 cut(s) 1308
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 1837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.