Rorug02G0620300

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000953
Physical Location & Seq
Reverse (-)
73751389 .. 73752809
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0620300.1

Sequence Viewer

Length: 939 bp
ATGTTGCCTGTTGAATCTGCAAAGAAGAAGCTATCTTTCACAAGATGTATTCAAGATGGGGATTTGGTTATTGTGTATGAGAGATATGATAACATGAAGGCTGTTAAAGTGTGTGAGGGTTCTGTGCTTGAGAATCGGTTTGGTGTGTTTAAGCATTCGGATTGGATAGGGAAGCAATTTGGGTCCAAAGTTTTCAGCAGCAAAGGTGGATTTGTTTACTTGTTGGCTCCCACTCCTGAGCTTTGGACTTTGGTTCTAAGTCACAGGACTCAGATTCTCTACATTGCTGATATTAGCTTTGTGATCATGTTCTTGGAGATAGTTCCTGGCTGTGTGGTTCTGGAGTCGGGGACTGGAAGTGGGTCTTTAACTACGTCACTTGCAAGGGCTGTAGCTCCTAAGGGACATGTGTATACATTTGATTTCCATGAGCAAAGGGCGGCCTCGGCTAGGGAGGACTTTGAAAAGACGGGGATAAGCAACACGGTCACAGTAGGAGTTAGAGATATTCAGGGTGAAGGCTTTCCAGATGAGTTTTCTGGGAGGGCAGATTCTGTCTTCCTGGACCTTCCCCAACCCTGGCTAGCCATCCCTTCAGTTGAGAAAATGTTAAAACAAGATGGGATTGTATGCTCCTTTTCCCCGTGCATTGAGCAGGTGCAGCGAGCATCGGAGACTTTGAGATCAAAGTTTACAGATATAAGGACATTTGAGATACTCCTTCGCACATATGAAGTTCGAGAAGGGAAAATGGATAGCTTCCAGGTCGAGGAAAGTGGTTCTGTTGGATCTCTACCATGTAAGAGGCGGCAACGATCGAGTGAAGGAAGTAATGAGCAGGAAACTACAAGCGCTTCTGTAGTCATGGCTAGGCCATGTAGTGAGGCCAAAGGCCACACTGGCTATTTGACATTTGCAAGGCTCAAATGCCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

312

Amino Acids

34.63

Weight (kDa)

6.55

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCD14 PF08704 70 - 307 4.2e-101 tRNA methyltransferase complex GCD14 subunit
PCMT PF01135 99 - 167 5.8e-06 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_31 PF13847 109 - 231 1.3e-10 Methyltransferase domain
Methyltransf_25 PF13649 113 - 188 2.7e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 646
Acc36I ACCTGC 1 cut(s) 646
AccI GTMKAC 1 cut(s) 413
AciI CCGC 2 cut(s) 440, 808
AclWI GGATC 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 579
AfeI AGCGCT 1 cut(s) 853
AfiI CCNNNNNNNGG 3 cut(s) 450, 579, 769
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 3 cut(s) 14, 53, 464
AjnI CCWGG 4 cut(s) 325, 561, 578, 762
AluBI AGCT 5 cut(s) 31, 241, 297, 395, 759
AluI AGCT 5 cut(s) 31, 241, 297, 395, 759
Alw26I GTCTC 1 cut(s) 668
AlwI GGATC 1 cut(s) 796
AlwNI CAGNNNCTG 1 cut(s) 554
Aor51HI AGCGCT 1 cut(s) 853
AoxI GGCC 4 cut(s) 441, 872, 885, 892
ApeKI GCWGC 2 cut(s) 198, 661
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 1 cut(s) 854
AspS9I GGNCC 2 cut(s) 183, 565
AsuHPI GGTGA 1 cut(s) 527
AsuNHI GCTAGC 1 cut(s) 583
AvaII GGWCC 2 cut(s) 183, 565
AxyI CCTNAGG 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 210, 673
BccI CCATC 3 cut(s) 50, 596, 614
BciT130I CCWGG 4 cut(s) 327, 563, 580, 764
BclI TGATCA 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 668
BfaI CTAG 3 cut(s) 450, 584, 870
BfmI CTRYAG 2 cut(s) 390, 858
BfoI RGCGCY 1 cut(s) 855
BfuAI ACCTGC 1 cut(s) 646
BglI GCCNNNNNGGC 1 cut(s) 900
BisI GCNGC 4 cut(s) 199, 441, 662, 809
BlsI GCNGC 4 cut(s) 200, 442, 663, 810
Bme1390I CCNGG 4 cut(s) 327, 563, 580, 764
Bme18I GGWCC 2 cut(s) 183, 565
BmgT120I GGNCC 2 cut(s) 183, 565
BmiI GGNNCC 2 cut(s) 184, 228
BmrFI CCNGG 4 cut(s) 327, 563, 580, 764
BmsI GCATC 1 cut(s) 677
BmtI GCTAGC 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 550
BpmI CTGGAG 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 444, 578
Bsc4I CCNNNNNNNGG 3 cut(s) 450, 579, 769
Bse1I ACTGG 2 cut(s) 358, 904
Bse21I CCTNAGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 4 cut(s) 327, 563, 580, 764
BseDI CCNNGG 2 cut(s) 444, 578
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 450, 579, 769
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 2 cut(s) 228, 284
BseNI ACTGG 2 cut(s) 358, 904
BseXI GCAGC 2 cut(s) 210, 673
BsgI GTGCAG 1 cut(s) 680
Bsh1285I CGRYCG 1 cut(s) 818
BshFI GGCC 4 cut(s) 443, 874, 887, 894
BsiEI CGRYCG 1 cut(s) 818
BslFI GGGAC 2 cut(s) 364, 417
BslI CCNNNNNNNGG 3 cut(s) 450, 579, 769
BsmAI GTCTC 1 cut(s) 668
BsmFI GGGAC 2 cut(s) 364, 417
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 4 cut(s) 443, 874, 887, 894
Bsp143I GATC 4 cut(s) 303, 683, 788, 815
BspACI CCGC 2 cut(s) 440, 808
BspANI GGCC 4 cut(s) 443, 874, 887, 894
BspCNI CTCAG 2 cut(s) 229, 283
BspLI GGNNCC 2 cut(s) 184, 228
BspMI ACCTGC 1 cut(s) 646
BspOI GCTAGC 1 cut(s) 587
BspPI GGATC 1 cut(s) 796
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 2 cut(s) 358, 904
BssECI CCNNGG 2 cut(s) 444, 578
BssMI GATC 4 cut(s) 303, 683, 788, 815
BssNAI GTATAC 1 cut(s) 414
Bst1107I GTATAC 1 cut(s) 414
Bst2UI CCWGG 4 cut(s) 327, 563, 580, 764
Bst4CI ACNGT 2 cut(s) 487, 493
BstC8I GCNNGC 2 cut(s) 585, 666
BstDEI CTNAG 4 cut(s) 237, 257, 270, 399
BstENI CCTNNNNNAGG 1 cut(s) 448
BstF5I GGATG 1 cut(s) 588
BstH2I RGCGCY 1 cut(s) 855
BstHHI GCGC 1 cut(s) 854
BstKTI GATC 4 cut(s) 306, 686, 791, 818
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 4 cut(s) 303, 683, 788, 815
BstMCI CGRYCG 1 cut(s) 818
BstMWI GCNNNNNNNGC 3 cut(s) 446, 661, 900
BstNI CCWGG 4 cut(s) 327, 563, 580, 764
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 4 cut(s) 325, 561, 578, 762
BstSFI CTRYAG 2 cut(s) 390, 858
BstV1I GCAGC 2 cut(s) 210, 673
BstV2I GAAGAC 1 cut(s) 550
BstX2I RGATCY 1 cut(s) 788
BstYI RGATCY 1 cut(s) 788
BstZ17I GTATAC 1 cut(s) 414
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 4 cut(s) 443, 874, 887, 894
BtsCI GGATG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 897
BveI ACCTGC 1 cut(s) 646
Cac8I GCNNGC 2 cut(s) 585, 666
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 1 cut(s) 854
Cfr13I GGNCC 2 cut(s) 183, 565
CviAII CATG 7 cut(s) 94, 307, 407, 428, 798, 865, 876
DdeI CTNAG 4 cut(s) 237, 257, 270, 399
DpnI GATC 4 cut(s) 305, 685, 790, 817
DpnII GATC 4 cut(s) 303, 683, 788, 815
Eco47I GGWCC 2 cut(s) 183, 565
Eco47III AGCGCT 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 579
Eco81I CCTNAGG 1 cut(s) 399
EcoNI CCTNNNNNAGG 1 cut(s) 448
EcoRII CCWGG 4 cut(s) 325, 561, 578, 762
FaeI CATG 7 cut(s) 97, 310, 410, 431, 801, 868, 879
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 2 cut(s) 364, 417
FatI CATG 7 cut(s) 93, 306, 406, 427, 797, 864, 875
FauNDI CATATG 1 cut(s) 730
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 4 cut(s) 199, 441, 662, 809
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 4 cut(s) 199, 441, 662, 809
FspBI CTAG 3 cut(s) 450, 584, 870
GlaI GCGC 1 cut(s) 853
GluI GCNGC 4 cut(s) 199, 441, 662, 809
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 855
HaeIII GGCC 4 cut(s) 443, 874, 887, 894
HhaI GCGC 1 cut(s) 854
Hin1II CATG 7 cut(s) 97, 310, 410, 431, 801, 868, 879
Hin6I GCGC 1 cut(s) 852
HinP1I GCGC 1 cut(s) 852
HinfI GANTC 6 cut(s) 14, 133, 268, 274, 344, 551
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 3 cut(s) 217, 414, 693
Hpy188I TCNGA 3 cut(s) 160, 273, 673
Hpy188III TCNNGA 5 cut(s) 53, 236, 341, 527, 740
Hpy8I GTNNAC 3 cut(s) 217, 414, 693
HpyAV CCTTC 7 cut(s) 91, 512, 578, 603, 731, 737, 818
HpyCH4III ACNGT 2 cut(s) 487, 493
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 5 cut(s) 20, 383, 648, 661, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 661, 900
HpyF3I CTNAG 4 cut(s) 237, 257, 270, 399
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 7 cut(s) 97, 310, 410, 431, 801, 868, 879
HspAI GCGC 1 cut(s) 852
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 4 cut(s) 303, 683, 788, 815
LmnI GCTCC 3 cut(s) 232, 400, 638
Lsp1109I GCAGC 2 cut(s) 210, 673
LweI GCATC 1 cut(s) 677
MaeI CTAG 3 cut(s) 450, 584, 870
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 3 cut(s) 260, 375, 487
MalI GATC 4 cut(s) 305, 685, 790, 817
MboI GATC 4 cut(s) 303, 683, 788, 815
MboII GAAGA 2 cut(s) 37, 550
MflI RGATCY 1 cut(s) 788
MluCI AATT 1 cut(s) 176
MlyI GAGTC 2 cut(s) 262, 353
MmeI TCCRAC 1 cut(s) 766
MnlI CCTC 7 cut(s) 109, 448, 454, 537, 763, 798, 877
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 4 cut(s) 105, 150, 368, 611
MspR9I CCNGG 4 cut(s) 327, 563, 580, 764
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 4 cut(s) 327, 563, 580, 764
MwoI GCNNNNNNNGC 3 cut(s) 446, 661, 900
NdeI CATATG 1 cut(s) 730
NdeII GATC 4 cut(s) 303, 683, 788, 815
NheI GCTAGC 1 cut(s) 583
NlaIII CATG 7 cut(s) 97, 310, 410, 431, 801, 868, 879
NlaIV GGNNCC 2 cut(s) 184, 228
NmeAIII GCCGAG 1 cut(s) 425
NmuCI GTSAC 3 cut(s) 260, 375, 487
NspI RCATGY 1 cut(s) 410
PaqCI CACCTGC 1 cut(s) 646
PciI ACATGT 1 cut(s) 406
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 4 cut(s) 14, 133, 274, 551
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 4 cut(s) 200, 442, 663, 810
Ple19I CGATCG 1 cut(s) 818
PleI GAGTC 2 cut(s) 262, 352
PpsI GAGTC 2 cut(s) 262, 352
PscI ACATGT 1 cut(s) 406
Psp6I CCWGG 4 cut(s) 325, 561, 578, 762
PspGI CCWGG 4 cut(s) 325, 561, 578, 762
PspN4I GGNNCC 2 cut(s) 184, 228
PspPI GGNCC 2 cut(s) 183, 565
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 788
PvuI CGATCG 1 cut(s) 818
SaqAI TTAA 4 cut(s) 105, 150, 368, 611
SatI GCNGC 4 cut(s) 199, 441, 662, 809
Sau3AI GATC 4 cut(s) 303, 683, 788, 815
Sau96I GGNCC 2 cut(s) 183, 565
SchI GAGTC 2 cut(s) 262, 353
ScrFI CCNGG 4 cut(s) 327, 563, 580, 764
SfaNI GCATC 1 cut(s) 677
SfcI CTRYAG 2 cut(s) 390, 858
SinI GGWCC 2 cut(s) 183, 565
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 176
SsiI CCGC 2 cut(s) 440, 808
SspMI CTAG 3 cut(s) 450, 584, 870
StyD4I CCNGG 4 cut(s) 325, 561, 578, 762
TaaI ACNGT 2 cut(s) 487, 493
TaiI ACGT 1 cut(s) 377
TaqI TCGA 3 cut(s) 739, 768, 818
TasI AATT 1 cut(s) 176
TauI GCSGC 2 cut(s) 443, 811
TfiI GAWTC 4 cut(s) 14, 133, 274, 551
Tru1I TTAA 4 cut(s) 105, 150, 368, 611
Tru9I TTAA 4 cut(s) 105, 150, 368, 611
TscAI CASTG 1 cut(s) 904
TseFI GTSAC 3 cut(s) 260, 375, 487
TseI GCWGC 2 cut(s) 198, 661
Tsp45I GTSAC 3 cut(s) 260, 375, 487
TspDTI ATGAA 3 cut(s) 110, 747, 924
TspRI CASTG 1 cut(s) 904
VpaK11BI GGWCC 2 cut(s) 183, 565
XagI CCTNNNNNAGG 1 cut(s) 448
XceI RCATGY 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 3 cut(s) 450, 584, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.