MD12G1251200.v1.1

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000953
Physical Location & Seq
Forward (+)
32094501 .. 32096617
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1251200.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGATACTCTGCTTCTGCCACTATTTTTCTTGCTTATAATTGTCTTCCCAATGGCCATGATTGTACCCAGCAGAGCAGCTGATCCTCATGACTGCCCGGTTACAAGGTGTAGGCCTGATGGTCCAGTTATCCGGTTTCCATTCCGTCTAAAACACCATCCTCCTTACTGTGGCCATCCAGAGTTCGAAGTTTTCTGCTCACAAAATATGACCATGATTAAGCTCTTGCCGCTTTCGGGAACCTTTCCTATACAATCTATTGATTACCAAAAAGATCAGTTTCAGATCTACGATGCGGATGGTTGCCTCCCGAAACACCTTCTTAATTCTACCATCTCCACGTACTCACTCTTTCAACCAGTAAACAATTTTGTGTTTTACAACAGTTGCGGTTTTGATCTCGAATATTATCCAGACTTATTGAACGTAACACTTTTAAATTGTAGCACAGCACAAAGTTTTAGCACAGAGAGCTCCCATGTGTACTTCATGCCGATTAAATGTCTCAGCGTCCCGGGGAGTCATCAAGTTCTAGCTGCTCTACAGAGTACTACTGCAGATAAACTGCCGATGTCTACTTGCACTCTTTTGAGACAGCTTGATCTTCCAATACGATATGTGGACGGTTCTATGCACGACTGTGAACCGAGCAAGGTTTTATTCCTGCAATGGAAGAAGTCATATTATCTGCGATCTACCTCATTGTCTATCCCTATCCCAGCATGTCAAAACTGCAGTACCTATGAAGACTGCGATATCATTACCGACACAAATCAAATGCAGTGTTTTCCTTATGATAGCGTGGCAGCAGTCCCGAAAGGTGTATCAACTGGATCTATTATCATAGGAGTAAGCATAGCTTGTTTTGGTCTCCTATCGTTGACAGCGGCAGCCATCCTCTACCATGTAAAAAAATCAAAAGGGAGAGAGGAAAAGGAGAATGAAATCGAGATTGAGAGGTTCCTAAAAGATCACAAATCTCATGTACCAACAAGATACACACATGGTGATATAAAGAGGATGACAAACGGATTCAAGAAGAAGTTAGGCGAAGGAGGTTTCGGAAGTGTTTACAGTGGAGAGCTTCCAAATGGAGTTCCGGTTGCCATAAAAGTCCTCAATGATTCTAAAGGAAAGGGGGAAGATTTTATTAATGAAGTGGGAACAATTGGCAGGATTCACCATGTTAACGTGGTTCGTCTACTTGGGTTTGCTGCAGGAGGAGGCAAGCGTGCTGTCATTTATGAGCTCATGCCGAACGGGTCTCTAGAGAAGTTTATCTCTACTACAGAACCCAACAAGAACCCACTAGATTGGGAGAAAATTCACAGCATCGTCGCTGGTATAGCAAAGGGAATCGAGTATCTTCACCAAGGGTGTGACCAGAGGATCCTCCACTTTGACATCAAGCCACGTAACATACTGTTAGACGATGACTTCAATCCTAAGATCTCGGATTTTGGTGTTGCTAAACTGTGTTCCAAGGGAGACAGTATCATATCCATGACGGCTGCTAGGGGCACAGTAGGGTATATTGCACCAGAAGTGTTTAATGGGAACTTCGGGAGCGTGTCCTACAAATCAGATGTCTATAGTTTTGGGATGCTAGTGTTTGAAATTGTTGGAGCCAAAAAACAACCTGCTCTTACATCTGGAAACAATGAGGTCTACTTTCCTGAATTGGTTTACAACCGTCTAGTTCAAGGAGAAGCTTTGGATTTGAAGCTAGATACCGATGAAGATGCTCAGATTGCTAAGAAACTAGTGATTGTGGCACTTTGGTGCATCCAGTGGTACCCGGTAAATCGCCCTTCCATGAAAGCGGTTGTTAGAATGCTAGAAGGAGGCCTTGAAAACTTGATGATGCCACCAAATCCATTTCCTTCCACAAGTACTCAGACAGATCAACCAAGAACAACACTGTTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

644

Amino Acids

71.51

Weight (kDa)

6.5

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 32 - 98 1.6e-14 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 345 - 613 9.5e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 346 - 611 8.7e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 1648
Acc65I GGTACC 1 cut(s) 1794
AccB1I GGYRCC 1 cut(s) 1794
AccI GTMKAC 3 cut(s) 575, 1201, 1668
AciI CCGC 5 cut(s) 230, 296, 390, 887, 1823
AclWI GGATC 4 cut(s) 77, 841, 1384, 1397
AcoI YGGCCR 2 cut(s) 54, 172
AcsI RAATTY 1 cut(s) 1323
AdeI CACNNNGTG 1 cut(s) 1007
AfaI GTAC 8 cut(s) 66, 344, 485, 550, 739, 987, 1796, 1894
AfiI CCNNNNNNNGG 2 cut(s) 170, 236
AgsI TTSAA 8 cut(s) 356, 424, 1036, 1441, 1616, 1703, 1723, 1853
AhlI ACTAGT 1 cut(s) 1762
AleI CACNNNNGTG 2 cut(s) 639, 1780
Alw21I GWGCWC 2 cut(s) 476, 1251
Alw26I GTCTC 5 cut(s) 509, 586, 875, 1269, 1482
AlwI GGATC 4 cut(s) 77, 841, 1384, 1397
Ama87I CYCGRG 1 cut(s) 514
AoxI GGCC 4 cut(s) 54, 113, 172, 1846
ApeKI GCWGC 6 cut(s) 77, 536, 806, 890, 1214, 1511
ApoI RAATTY 1 cut(s) 1323
ArsI GACNNNNNNTTYG 2 cut(s) 1572, 1604
AseI ATTAAT 1 cut(s) 1152
Asp718I GGTACC 1 cut(s) 1794
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 4 cut(s) 98, 515, 516, 1799
AsuHPI GGTGA 3 cut(s) 1019, 1172, 1361
AsuII TTCGAA 1 cut(s) 186
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 1523
BaeI ACNNNNGTAYC 2 cut(s) 721, 754
BalI TGGCCA 2 cut(s) 56, 174
BamHI GGATCC 1 cut(s) 1389
BanI GGYRCC 1 cut(s) 1794
BanII GRGCYC 2 cut(s) 476, 1251
BbsI GAAGAC 2 cut(s) 37, 753
Bbv12I GWGCWC 2 cut(s) 476, 1251
BbvI GCAGC 6 cut(s) 89, 523, 818, 902, 1201, 1498
BccI CCATC 6 cut(s) 113, 165, 183, 293, 341, 902
BceAI ACGGC 1 cut(s) 1524
BcgI CGANNNNNNTGC 2 cut(s) 1724, 1758
BcnI CCSGG 4 cut(s) 98, 515, 516, 1799
BcoDI GTCTC 5 cut(s) 509, 586, 875, 1269, 1482
BcuI ACTAGT 1 cut(s) 1762
BfaI CTAG 9 cut(s) 533, 1268, 1310, 1515, 1607, 1697, 1727, 1763, 1838
BfmI CTRYAG 6 cut(s) 542, 555, 733, 1215, 1287, 1591
BfuAI ACCTGC 1 cut(s) 1648
BglII AGATCT 2 cut(s) 285, 1449
BisI GCNGC 8 cut(s) 78, 230, 537, 807, 888, 891, 1215, 1512
BlsI GCNGC 8 cut(s) 79, 231, 538, 808, 889, 892, 1216, 1513
BmcAI AGTACT 2 cut(s) 550, 1894
Bme1390I CCNGG 4 cut(s) 98, 515, 516, 1799
Bme18I GGWCC 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 5 cut(s) 241, 962, 1391, 1627, 1796
BmrFI CCNGG 4 cut(s) 98, 515, 516, 1799
BmsI GCATC 6 cut(s) 283, 1341, 1593, 1732, 1794, 1854
BpiI GAAGAC 2 cut(s) 37, 753
Bpu14I TTCGAA 1 cut(s) 186
BpuMI CCSGG 4 cut(s) 98, 515, 516, 1799
BsaAI YACGTR 2 cut(s) 342, 1415
BsaI GGTCTC 2 cut(s) 875, 1269
BsaJI CCNNGG 4 cut(s) 514, 515, 1372, 1482
BsaWI WCCGGW 2 cut(s) 132, 1099
BsaXI ACNNNNNCTCC 2 cut(s) 1086, 1116
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 236
Bse1I ACTGG 4 cut(s) 125, 359, 835, 1789
Bse3DI GCAATG 1 cut(s) 674
BseDI CCNNGG 4 cut(s) 514, 515, 1372, 1482
BseGI GGATG 7 cut(s) 157, 175, 304, 894, 1026, 1608, 1785
BseLI CCNNNNNNNGG 2 cut(s) 170, 236
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 3 cut(s) 520, 1760, 1910
BseNI ACTGG 4 cut(s) 125, 359, 835, 1789
BseRI GAGGAG 1 cut(s) 1236
BseSI GKGCMC 1 cut(s) 1523
BseXI GCAGC 6 cut(s) 89, 523, 818, 902, 1201, 1498
BseYI CCCAGC 2 cut(s) 68, 718
BshFI GGCC 4 cut(s) 56, 115, 174, 1848
BshNI GGYRCC 1 cut(s) 1794
BsiHKAI GWGCWC 2 cut(s) 476, 1251
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 5 cut(s) 98, 133, 515, 1100, 1799
BslFI GGGAC 2 cut(s) 497, 797
BslI CCNNNNNNNGG 2 cut(s) 170, 236
BsmAI GTCTC 5 cut(s) 509, 586, 875, 1269, 1482
BsmFI GGGAC 2 cut(s) 497, 797
BsmI GAATGC 1 cut(s) 1839
BsnI GGCC 4 cut(s) 56, 115, 174, 1848
Bso31I GGTCTC 2 cut(s) 875, 1269
BsoBI CYCGRG 1 cut(s) 514
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 3 cut(s) 476, 1251, 1523
BspACI CCGC 5 cut(s) 230, 296, 390, 887, 1823
BspANI GGCC 4 cut(s) 56, 115, 174, 1848
BspCNI CTCAG 3 cut(s) 519, 1759, 1909
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 5 cut(s) 241, 962, 1391, 1627, 1796
BspMAI CTGCAG 3 cut(s) 559, 737, 1219
BspMI ACCTGC 1 cut(s) 1648
BspPI GGATC 4 cut(s) 77, 841, 1384, 1397
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 1794
BspTNI GGTCTC 2 cut(s) 875, 1269
BsrDI GCAATG 1 cut(s) 674
BsrI ACTGG 4 cut(s) 125, 359, 835, 1789
BssECI CCNNGG 4 cut(s) 514, 515, 1372, 1482
BssT1I CCWWGG 2 cut(s) 1372, 1482
BstBAI YACGTR 2 cut(s) 342, 1415
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 1229, 1233
BstDEI CTNAG 5 cut(s) 506, 1446, 1746, 1755, 1896
BstF5I GGATG 7 cut(s) 157, 175, 304, 894, 1026, 1608, 1785
BstMAI GTCTC 5 cut(s) 509, 586, 875, 1269, 1482
BstMWI GCNNNNNNNGC 4 cut(s) 229, 471, 1346, 1751
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 4 cut(s) 96, 513, 514, 1797
BstSFI CTRYAG 6 cut(s) 542, 555, 733, 1215, 1287, 1591
BstSLI GKGCMC 1 cut(s) 1523
BstV1I GCAGC 6 cut(s) 89, 523, 818, 902, 1201, 1498
BstV2I GAAGAC 2 cut(s) 37, 753
BstX2I RGATCY 4 cut(s) 285, 833, 1389, 1449
BstXI CCANNNNNNTGG 1 cut(s) 1314
BstYI RGATCY 4 cut(s) 285, 833, 1389, 1449
BsuRI GGCC 4 cut(s) 56, 115, 174, 1848
BtsCI GGATG 7 cut(s) 157, 175, 304, 894, 1026, 1608, 1785
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 4 cut(s) 788, 1081, 1796, 1919
BveI ACCTGC 1 cut(s) 1648
Cac8I GCNNGC 2 cut(s) 1229, 1233
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 122
Cfr9I CCCGGG 1 cut(s) 514
CseI GACGC 1 cut(s) 499
Csp6I GTAC 8 cut(s) 65, 343, 484, 549, 738, 986, 1795, 1893
CviQI GTAC 8 cut(s) 65, 343, 484, 549, 738, 986, 1795, 1893
DdeI CTNAG 5 cut(s) 506, 1446, 1746, 1755, 1896
DraI TTTAAA 1 cut(s) 438
DraIII CACNNNGTG 1 cut(s) 1007
EaeI YGGCCR 2 cut(s) 54, 172
Ecl136II GAGCTC 2 cut(s) 474, 1249
Eco130I CCWWGG 2 cut(s) 1372, 1482
Eco147I AGGCCT 2 cut(s) 115, 1848
Eco24I GRGCYC 2 cut(s) 476, 1251
Eco31I GGTCTC 2 cut(s) 875, 1269
Eco32I GATATC 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 2 cut(s) 474, 1249
Eco88I CYCGRG 1 cut(s) 514
EcoICRI GAGCTC 2 cut(s) 474, 1249
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 1372, 1482
EcoT38I GRGCYC 2 cut(s) 476, 1251
ErhI CCWWGG 2 cut(s) 1372, 1482
FalI AAGNNNNNCTT 4 cut(s) 844, 876, 1833, 1865
FaqI GGGAC 2 cut(s) 497, 797
FblI GTMKAC 3 cut(s) 575, 1201, 1668
Fnu4HI GCNGC 8 cut(s) 78, 230, 537, 807, 888, 891, 1215, 1512
FokI GGATG 7 cut(s) 144, 162, 311, 881, 1033, 1615, 1772
FriOI GRGCYC 2 cut(s) 476, 1251
Fsp4HI GCNGC 8 cut(s) 78, 230, 537, 807, 888, 891, 1215, 1512
FspBI CTAG 9 cut(s) 533, 1268, 1310, 1515, 1607, 1697, 1727, 1763, 1838
GluI GCNGC 8 cut(s) 78, 230, 537, 807, 888, 891, 1215, 1512
GsaI CCCAGC 2 cut(s) 72, 722
HaeIII GGCC 4 cut(s) 56, 115, 174, 1848
HapII CCGG 5 cut(s) 98, 133, 515, 1100, 1799
HgaI GACGC 1 cut(s) 499
HincII GTYRAC 2 cut(s) 882, 1189
HindII GTYRAC 2 cut(s) 882, 1189
HindIII AAGCTT 1 cut(s) 1710
HinfI GANTC 5 cut(s) 520, 1032, 1124, 1177, 1356
HpaI GTTAAC 1 cut(s) 1189
HpaII CCGG 5 cut(s) 98, 133, 515, 1100, 1799
HphI GGTGA 3 cut(s) 1019, 1172, 1361
Hpy188I TCNGA 6 cut(s) 285, 1064, 1456, 1585, 1749, 1899
Hpy99I CGWCG 1 cut(s) 1340
HpyAV CCTTC 5 cut(s) 329, 1046, 1821, 1835, 1893
HpyCH4IV ACGT 4 cut(s) 341, 426, 1191, 1414
HpyCH4V TGCA 9 cut(s) 557, 582, 634, 667, 735, 781, 1217, 1538, 1785
HpyF10VI GCNNNNNNNGC 4 cut(s) 229, 471, 1346, 1751
HpyF3I CTNAG 5 cut(s) 506, 1446, 1746, 1755, 1896
HpySE526I ACGT 4 cut(s) 341, 426, 1191, 1414
KpnI GGTACC 1 cut(s) 1798
KspAI GTTAAC 1 cut(s) 1189
LmnI GCTCC 3 cut(s) 479, 1566, 1625
Lsp1109I GCAGC 6 cut(s) 89, 523, 818, 902, 1201, 1498
LweI GCATC 6 cut(s) 283, 1341, 1593, 1732, 1794, 1854
MaeI CTAG 9 cut(s) 533, 1268, 1310, 1515, 1607, 1697, 1727, 1763, 1838
MaeII ACGT 4 cut(s) 341, 426, 1191, 1414
MaeIII GTNAC 4 cut(s) 100, 427, 1379, 1415
MboII GAAGA 8 cut(s) 37, 596, 685, 758, 1051, 1154, 1358, 1751
MfeI CAATTG 1 cut(s) 1167
MflI RGATCY 4 cut(s) 285, 833, 1389, 1449
MhlI GDGCHC 3 cut(s) 476, 1251, 1523
MlsI TGGCCA 2 cut(s) 56, 174
MluCI AATT 8 cut(s) 39, 325, 367, 439, 1167, 1323, 1617, 1679
MluNI TGGCCA 2 cut(s) 56, 174
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 1603
Mox20I TGGCCA 2 cut(s) 56, 174
MscI TGGCCA 2 cut(s) 56, 174
MseI TTAA 9 cut(s) 219, 324, 437, 498, 1152, 1188, 1551, 1925, 1930
MslI CAYNNNNRTG 3 cut(s) 639, 1502, 1780
Msp20I TGGCCA 2 cut(s) 56, 174
MspA1I CMGCKG 2 cut(s) 80, 887
MspI CCGG 5 cut(s) 98, 133, 515, 1100, 1799
MspR9I CCNGG 4 cut(s) 98, 515, 516, 1799
MunI CAATTG 1 cut(s) 1167
Mva1269I GAATGC 1 cut(s) 1839
MwoI GCNNNNNNNGC 4 cut(s) 229, 471, 1346, 1751
NciI CCSGG 4 cut(s) 98, 515, 516, 1799
NlaIV GGNNCC 5 cut(s) 241, 962, 1391, 1627, 1796
NmuCI GTSAC 1 cut(s) 1379
NspI RCATGY 1 cut(s) 726
NspV TTCGAA 1 cut(s) 186
OliI CACNNNNGTG 2 cut(s) 639, 1780
PagI TCATGA 1 cut(s) 88
PceI AGGCCT 2 cut(s) 115, 1848
PctI GAATGC 1 cut(s) 1839
PfeI GAWTC 4 cut(s) 1032, 1124, 1177, 1356
PkrI GCNGC 8 cut(s) 79, 231, 538, 808, 889, 892, 1216, 1513
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
Ppu21I YACGTR 2 cut(s) 342, 1415
PshBI ATTAAT 1 cut(s) 1152
PsiI TTATAA 1 cut(s) 38
Psp124BI GAGCTC 2 cut(s) 476, 1251
PspFI CCCAGC 2 cut(s) 68, 718
PspN4I GGNNCC 5 cut(s) 241, 962, 1391, 1627, 1796
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 3 cut(s) 559, 737, 1219
PsuI RGATCY 4 cut(s) 285, 833, 1389, 1449
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 8 cut(s) 66, 344, 485, 550, 739, 987, 1796, 1894
RsaNI GTAC 8 cut(s) 65, 343, 484, 549, 738, 986, 1795, 1893
RseI CAYNNNNRTG 3 cut(s) 639, 1502, 1780
SacI GAGCTC 2 cut(s) 476, 1251
SaqAI TTAA 9 cut(s) 219, 324, 437, 498, 1152, 1188, 1551, 1925, 1930
SatI GCNGC 8 cut(s) 78, 230, 537, 807, 888, 891, 1215, 1512
Sau96I GGNCC 1 cut(s) 122
ScaI AGTACT 2 cut(s) 550, 1894
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 4 cut(s) 98, 515, 516, 1799
SduI GDGCHC 3 cut(s) 476, 1251, 1523
SfaNI GCATC 6 cut(s) 283, 1341, 1593, 1732, 1794, 1854
SfcI CTRYAG 6 cut(s) 542, 555, 733, 1215, 1287, 1591
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 122
SmaI CCCGGG 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 639, 1502, 1780
SpeI ACTAGT 1 cut(s) 1762
Sse9I AATT 8 cut(s) 39, 325, 367, 439, 1167, 1323, 1617, 1679
SseBI AGGCCT 2 cut(s) 115, 1848
SsiI CCGC 5 cut(s) 230, 296, 390, 887, 1823
SspI AATATT 1 cut(s) 407
SspMI CTAG 9 cut(s) 533, 1268, 1310, 1515, 1607, 1697, 1727, 1763, 1838
SstI GAGCTC 2 cut(s) 476, 1251
StuI AGGCCT 2 cut(s) 115, 1848
StyD4I CCNGG 4 cut(s) 96, 513, 514, 1797
StyI CCWWGG 2 cut(s) 1372, 1482
TaiI ACGT 4 cut(s) 344, 429, 1194, 1417
TaqI TCGA 4 cut(s) 186, 402, 948, 1359
TasI AATT 8 cut(s) 39, 325, 367, 439, 1167, 1323, 1617, 1679
TatI WGTACW 3 cut(s) 483, 548, 1892
TauI GCSGC 2 cut(s) 232, 890
TfiI GAWTC 4 cut(s) 1032, 1124, 1177, 1356
Tru1I TTAA 9 cut(s) 219, 324, 437, 498, 1152, 1188, 1551, 1925, 1930
Tru9I TTAA 9 cut(s) 219, 324, 437, 498, 1152, 1188, 1551, 1925, 1930
TscAI CASTG 4 cut(s) 788, 1081, 1796, 1926
TseFI GTSAC 1 cut(s) 1379
TseI GCWGC 6 cut(s) 77, 536, 806, 890, 1214, 1511
Tsp45I GTSAC 1 cut(s) 1379
TspDTI ATGAA 6 cut(s) 478, 759, 957, 1170, 1752, 1832
TspGWI ACGGA 2 cut(s) 134, 1044
TspMI CCCGGG 1 cut(s) 514
TspRI CASTG 4 cut(s) 788, 1081, 1796, 1926
VpaK11BI GGWCC 1 cut(s) 122
VspI ATTAAT 1 cut(s) 1152
XapI RAATTY 1 cut(s) 1323
XbaI TCTAGA 1 cut(s) 1267
XceI RCATGY 1 cut(s) 726
XmaI CCCGGG 1 cut(s) 514
XmiI GTMKAC 3 cut(s) 575, 1201, 1668
XspI CTAG 9 cut(s) 533, 1268, 1310, 1515, 1607, 1697, 1727, 1763, 1838
ZrmI AGTACT 2 cut(s) 550, 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.