RLG00000025852

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000953
Physical Location & Seq
Forward (+)
48686653 .. 48687969
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025852

Sequence Viewer

Length: 1155 bp
ATGGAGTGTTACAATTATTCTAGTTATCTGTATCCCCCACAAAAGCTGAAAGGTACTAGGTCAATTGCCATAGGGGTGACCACAGGTTGCTTTCTTCTCGTATCCACAGCGGTAGTAGCCATCTTCTACTACATGAGACAAACAAACAGAACAGAGCAAAAGGAAAATCAACAAAAGATTGAAAAGTTCCTAGATGATCACAAGTCCCATGTACCAACAAGATACTCCTATGCTGATATAAAGAAGATGACAAATGGATTCAAGAAGAAATTGGGTGAAGGAGGTTTTGGAAGTGTTTTCAGTGGAAAGCTTCCCAGTAATGGAGTTTTGGTCGCCGTAAAAGTCCTCAAGGATTCTAAAGGAAATGGAGATGATTTTGTTAATGAAATGGGAACCATTGGCAGAATTCACCATGTCAATGTGGTTCGCCTACTTGGCTTTTCTGCTGAAGGAGGGAAGCGTGCTCTTATTTATGAGCTCATGCCAAACAGCTCCCTAGAGAAGTTTATATCTTCTGCACGATCCAACGATACTCCATTTGATTGGGAGAAACTTCGCAACATCATCATCGGTATAGCAAAGGGAATTGAGTATCTACACCAAGGGTGCGACCAAAGGATCCTCCATTTTGATATCAAGCCTCATAATATCTTGTTAGACCATGACTTCAATCCAAAAATCTCGGATTTTGGTCTCGCTAAACTCTACTCCAAGGAAGAGAGTATGATATCTATGACCGCTGCAAGAGGCACTGTAGGCTACATTGCCCCAGAAGTGTTCAATGGCTACTTTGGAACCGTCTCCTACAAATCGGATGTGTATAGTTTTGGGATGCTAGTGCTCGAAATCATTGGAGCTAGAAAGGAATCTGCTCTTACATCTAACAACAGCCAGGTGTATTTTCCTGAATTGATCTATGAGTGTCTAAATCAAGGGGAGAAACTGGATTTAGAGCTAGGTGACGATGGAGATGCTCAGATTGCTAAGCGACTAGCGATTGTTGCACTTTGGTGTATTCAGTGGTACCCAGTGAATCGACCTTCCATGAAAGGGGTTGTGAGAATGCTAGCAGGGGCATCTGAAAACTTAATGATGCCACCCAATCCGTTCGCTTCCACAACTAAAGCGGAACCAACAACAGCACTTCCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

385

Amino Acids

42.81

Weight (kDa)

8.57

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 87 - 356 3.6e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 88 - 353 3e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1023
AccB1I GGYRCC 1 cut(s) 1023
AciI CCGC 3 cut(s) 110, 738, 1127
AclWI GGATC 3 cut(s) 516, 613, 626
AcsI RAATTY 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 3 cut(s) 55, 213, 1025
AfiI CCNNNNNNNGG 2 cut(s) 320, 1050
AgsI TTSAA 4 cut(s) 182, 262, 670, 781
AjnI CCWGG 1 cut(s) 891
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AleI CACNNNNGTG 1 cut(s) 1009
AluBI AGCT 6 cut(s) 46, 310, 478, 492, 857, 955
AluI AGCT 6 cut(s) 46, 310, 478, 492, 857, 955
Alw21I GWGCWC 3 cut(s) 466, 480, 843
Alw26I GTCTC 3 cut(s) 130, 698, 805
AlwI GGATC 3 cut(s) 516, 613, 626
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 1 cut(s) 405
Asp718I GGTACC 1 cut(s) 1023
AsuHPI GGTGA 4 cut(s) 88, 287, 401, 971
AsuNHI GCTAGC 1 cut(s) 1066
BamHI GGATCC 1 cut(s) 618
BanI GGYRCC 1 cut(s) 1023
BanII GRGCYC 1 cut(s) 480
Bbv12I GWGCWC 3 cut(s) 466, 480, 843
BbvI GCAGC 1 cut(s) 727
BccI CCATC 2 cut(s) 128, 959
BceAI ACGGC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 1 cut(s) 893
BciVI GTATCC 2 cut(s) 42, 112
BclI TGATCA 1 cut(s) 196
BcoDI GTCTC 3 cut(s) 130, 698, 805
BfaI CTAG 9 cut(s) 21, 57, 191, 497, 836, 858, 956, 992, 1067
BfmI CTRYAG 1 cut(s) 753
BfuI GTATCC 2 cut(s) 42, 112
BglI GCCNNNNNGGC 1 cut(s) 435
BisI GCNGC 1 cut(s) 741
BlpI GCTNAGC 1 cut(s) 984
BlsI GCNGC 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 893
BmiI GGNNCC 5 cut(s) 394, 620, 796, 1025, 1131
BmrFI CCNGG 1 cut(s) 893
BmrI ACTGGG 2 cut(s) 309, 1022
BmsI GCATC 4 cut(s) 822, 961, 1083, 1085
BmtI GCTAGC 1 cut(s) 1070
BmuI ACTGGG 2 cut(s) 309, 1022
Bpu1102I GCTNAGC 1 cut(s) 984
BpuEI CTTGAG 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 2 cut(s) 601, 711
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 1050
Bse1I ACTGG 3 cut(s) 315, 948, 1028
Bse3DI GCAATG 1 cut(s) 762
BseBI CCWGG 1 cut(s) 893
BseDI CCNNGG 2 cut(s) 601, 711
BseGI GGATG 2 cut(s) 820, 837
BseLI CCNNNNNNNGG 2 cut(s) 320, 1050
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 1 cut(s) 989
BseNI ACTGG 3 cut(s) 315, 948, 1028
BseXI GCAGC 1 cut(s) 727
BsgI GTGCAG 1 cut(s) 501
BshNI GGYRCC 1 cut(s) 1023
BsiHKAI GWGCWC 3 cut(s) 466, 480, 843
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 2 cut(s) 320, 1050
BsmAI GTCTC 3 cut(s) 130, 698, 805
BsmBI CGTCTC 1 cut(s) 805
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 1068
Bso31I GGTCTC 1 cut(s) 698
Bsp1286I GDGCHC 3 cut(s) 466, 480, 843
Bsp143I GATC 4 cut(s) 196, 521, 618, 912
Bsp1720I GCTNAGC 1 cut(s) 984
BspACI CCGC 3 cut(s) 110, 738, 1127
BspCNI CTCAG 1 cut(s) 988
BspLI GGNNCC 5 cut(s) 394, 620, 796, 1025, 1131
BspOI GCTAGC 1 cut(s) 1070
BspPI GGATC 3 cut(s) 516, 613, 626
BspT107I GGYRCC 1 cut(s) 1023
BspTNI GGTCTC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 3 cut(s) 315, 948, 1028
BssECI CCNNGG 2 cut(s) 601, 711
BssMI GATC 4 cut(s) 196, 521, 618, 912
BssT1I CCWWGG 2 cut(s) 601, 711
Bst2UI CCWGG 1 cut(s) 893
Bst4CI ACNGT 2 cut(s) 754, 799
Bst6I CTCTTC 1 cut(s) 711
BstC8I GCNNGC 2 cut(s) 462, 1068
BstDEI CTNAG 2 cut(s) 975, 984
BstEII GGTNACC 1 cut(s) 76
BstF5I GGATG 2 cut(s) 820, 837
BstKTI GATC 4 cut(s) 199, 524, 621, 915
BstMAI GTCTC 3 cut(s) 130, 698, 805
BstMBI GATC 4 cut(s) 196, 521, 618, 912
BstMWI GCNNNNNNNGC 5 cut(s) 116, 435, 756, 980, 1001
BstNI CCWGG 1 cut(s) 893
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 891
BstSFI CTRYAG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 727
BstX2I RGATCY 1 cut(s) 618
BstXI CCANNNNNNTGG 1 cut(s) 543
BstYI RGATCY 1 cut(s) 618
BsuI GTATCC 2 cut(s) 42, 112
BtsCI GGATG 2 cut(s) 820, 837
BtsIMutI CAGTG 4 cut(s) 307, 750, 1025, 1035
Cac8I GCNNGC 2 cut(s) 462, 1068
Csp6I GTAC 3 cut(s) 54, 212, 1024
CviAII CATG 6 cut(s) 133, 209, 413, 481, 662, 1045
CviQI GTAC 3 cut(s) 54, 212, 1024
DdeI CTNAG 2 cut(s) 975, 984
DpnI GATC 4 cut(s) 198, 523, 620, 914
DpnII GATC 4 cut(s) 196, 521, 618, 912
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Ecl136II GAGCTC 1 cut(s) 478
Eco130I CCWWGG 2 cut(s) 601, 711
Eco24I GRGCYC 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 698
Eco32I GATATC 2 cut(s) 634, 729
Eco53kI GAGCTC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 468
Eco91I GGTNACC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 478
EcoO65I GGTNACC 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 891
EcoRV GATATC 2 cut(s) 634, 729
EcoT14I CCWWGG 2 cut(s) 601, 711
EcoT38I GRGCYC 1 cut(s) 480
ErhI CCWWGG 2 cut(s) 601, 711
Esp3I CGTCTC 1 cut(s) 805
FaeI CATG 6 cut(s) 136, 212, 416, 484, 665, 1048
FaqI GGGAC 1 cut(s) 190
FatI CATG 6 cut(s) 132, 208, 412, 480, 661, 1044
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 2 cut(s) 827, 844
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 9 cut(s) 21, 57, 191, 497, 836, 858, 956, 992, 1067
GluI GCNGC 1 cut(s) 741
Hin1II CATG 6 cut(s) 136, 212, 416, 484, 665, 1048
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 4 cut(s) 258, 353, 866, 1033
HphI GGTGA 4 cut(s) 88, 287, 401, 971
Hpy188I TCNGA 4 cut(s) 685, 814, 978, 1081
Hpy188III TCNNGA 2 cut(s) 262, 905
HpyAV CCTTC 3 cut(s) 272, 443, 1050
HpyCH4III ACNGT 2 cut(s) 754, 799
HpyCH4V TGCA 3 cut(s) 518, 743, 1004
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 435, 756, 980, 1001
HpyF3I CTNAG 2 cut(s) 975, 984
Hsp92II CATG 6 cut(s) 136, 212, 416, 484, 665, 1048
KpnI GGTACC 1 cut(s) 1027
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 4 cut(s) 196, 521, 618, 912
LmnI GCTCC 2 cut(s) 497, 854
LpnPI CCDG 9 cut(s) 69, 328, 783, 878, 905, 918, 929, 1041, 1056
Lsp1109I GCAGC 1 cut(s) 727
LweI GCATC 4 cut(s) 822, 961, 1083, 1085
MaeI CTAG 9 cut(s) 21, 57, 191, 497, 836, 858, 956, 992, 1067
MaeIII GTNAC 3 cut(s) 8, 76, 959
MalI GATC 4 cut(s) 198, 523, 620, 914
MboI GATC 4 cut(s) 196, 521, 618, 912
MboII GAAGA 6 cut(s) 86, 115, 256, 277, 504, 728
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 1 cut(s) 618
MhlI GDGCHC 3 cut(s) 466, 480, 843
MluCI AATT 6 cut(s) 13, 63, 269, 405, 585, 908
MmeI TCCRAC 1 cut(s) 549
MnlI CCTC 6 cut(s) 275, 356, 446, 632, 651, 740
MseI TTAA 3 cut(s) 381, 1088, 1153
MslI CAYNNNNRTG 3 cut(s) 74, 417, 1009
MspA1I CMGCKG 2 cut(s) 110, 740
MspR9I CCNGG 1 cut(s) 893
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 1068
MvaI CCWGG 1 cut(s) 893
MwoI GCNNNNNNNGC 5 cut(s) 116, 435, 756, 980, 1001
NdeII GATC 4 cut(s) 196, 521, 618, 912
NheI GCTAGC 1 cut(s) 1066
NlaIII CATG 6 cut(s) 136, 212, 416, 484, 665, 1048
NlaIV GGNNCC 5 cut(s) 394, 620, 796, 1025, 1131
NmuCI GTSAC 2 cut(s) 76, 959
OliI CACNNNNGTG 1 cut(s) 1009
PctI GAATGC 1 cut(s) 1068
PfeI GAWTC 4 cut(s) 258, 353, 866, 1033
PkrI GCNGC 1 cut(s) 742
Psp124BI GAGCTC 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 891
PspEI GGTNACC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 891
PspN4I GGNNCC 5 cut(s) 394, 620, 796, 1025, 1131
PsuI RGATCY 1 cut(s) 618
RsaI GTAC 3 cut(s) 55, 213, 1025
RsaNI GTAC 3 cut(s) 54, 212, 1024
RseI CAYNNNNRTG 3 cut(s) 74, 417, 1009
SacI GAGCTC 1 cut(s) 480
SaqAI TTAA 3 cut(s) 381, 1088, 1153
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 4 cut(s) 196, 521, 618, 912
ScrFI CCNGG 1 cut(s) 893
SduI GDGCHC 3 cut(s) 466, 480, 843
SfaNI GCATC 4 cut(s) 822, 961, 1083, 1085
SfcI CTRYAG 1 cut(s) 753
SmiMI CAYNNNNRTG 3 cut(s) 74, 417, 1009
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 6 cut(s) 13, 63, 269, 405, 585, 908
SsiI CCGC 3 cut(s) 110, 738, 1127
SspMI CTAG 9 cut(s) 21, 57, 191, 497, 836, 858, 956, 992, 1067
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 891
StyI CCWWGG 2 cut(s) 601, 711
TaaI ACNGT 2 cut(s) 754, 799
TaqI TCGA 2 cut(s) 843, 1036
TasI AATT 6 cut(s) 13, 63, 269, 405, 585, 908
TfiI GAWTC 4 cut(s) 258, 353, 866, 1033
Tru1I TTAA 3 cut(s) 381, 1088, 1153
Tru9I TTAA 3 cut(s) 381, 1088, 1153
TscAI CASTG 4 cut(s) 307, 757, 1025, 1035
TseFI GTSAC 2 cut(s) 76, 959
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 2 cut(s) 76, 959
TspDTI ATGAA 2 cut(s) 399, 1061
TspGWI ACGGA 1 cut(s) 1095
TspRI CASTG 4 cut(s) 307, 757, 1025, 1035
XapI RAATTY 1 cut(s) 405
XspI CTAG 9 cut(s) 21, 57, 191, 497, 836, 858, 956, 992, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.