Rorug02G0618000

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000953
Physical Location & Seq
Forward (+)
73589616 .. 73591522
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0618000.1

Sequence Viewer

Length: 531 bp
ATGGCGGGTGCAGCACCGACTCGGGTACTGATGGGAGTGAACGAGTCGACGATCAAGGGCTACCCGCACGCCTCCATAAGCAGCCGCAAAGCCTTCGAGTGGACTCTCGATAAGATCGTCCGCTCCAACACCTCCGGCTTCAAGCTTCTCTTCCTCCATGTGCAAGTTCCTGACGAAGACGGTTTTGACGACATGGACAGTATCTATGCGTCACCGGAGGATTTTAAAAGCTTGAAGCATAGAGACCAGGCAAGAGGGGCTCATCTGCTGGAGTTCTTTGTTGAGAGATGTCATGCAATTGGGGTTGCTTGTGAAGCATGGATCAAGAAAGGTGATCCAAAGGAAGTAATCTGCCATGAGGTGAAACGATTGCAGCCAGATCTTCTAGTTGTTGGCTGCCGCGGTCTTGGTCCTTTCCAGAGGGTTTTTGTGGGGACTGTGAGTGAATTTTGCGTCAAGCATGCCGAATGCCCTGTCATCACAATCAAGCGCAGTGCCGAAGAGACACCTCAGGATCCAGTCGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

176

Amino Acids

19.61

Weight (kDa)

5.97

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 25 - 163 3.4e-20 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 47
AccII CGCG 1 cut(s) 402
AciI CCGC 6 cut(s) 5, 65, 85, 121, 400, 402
AclWI GGATC 4 cut(s) 329, 329, 509, 522
AcsI RAATTY 1 cut(s) 446
AfaI GTAC 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 99
AgsI TTSAA 2 cut(s) 142, 235
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 2 cut(s) 145, 231
AluI AGCT 2 cut(s) 145, 231
Alw26I GTCTC 2 cut(s) 237, 497
AlwI GGATC 4 cut(s) 329, 329, 509, 522
Ama87I CYCGRG 1 cut(s) 21
ApeKI GCWGC 4 cut(s) 11, 81, 373, 396
ApoI RAATTY 1 cut(s) 446
ArsI GACNNNNNNTTYG 2 cut(s) 459, 491
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 3 cut(s) 204, 344, 373
AvaI CYCGRG 1 cut(s) 21
AvaII GGWCC 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 510
BamHI GGATCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 4 cut(s) 23, 93, 383, 385
BccI CCATC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 248
BcoDI GTCTC 2 cut(s) 237, 497
BfaI CTAG 1 cut(s) 386
BglII AGATCT 1 cut(s) 379
BisI GCNGC 6 cut(s) 12, 82, 85, 374, 397, 400
BlsI GCNGC 6 cut(s) 13, 83, 86, 375, 398, 401
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 410
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 290
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 400
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse1I ACTGG 1 cut(s) 518
Bse21I CCTNAGG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 1 cut(s) 518
BseXI GCAGC 4 cut(s) 23, 93, 383, 385
BsgI GTGCAG 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 2 cut(s) 135, 215
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 237, 497
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 1 cut(s) 473
Bso31I GGTCTC 1 cut(s) 237
BsoBI CYCGRG 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 262
Bsp143I GATC 6 cut(s) 51, 114, 321, 334, 379, 514
BspACI CCGC 6 cut(s) 5, 65, 85, 121, 400, 402
BspCNI CTCAG 1 cut(s) 523
BspFNI CGCG 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 4 cut(s) 329, 329, 509, 522
BspTNI GGTCTC 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 123
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 6 cut(s) 51, 114, 321, 334, 379, 514
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 3 cut(s) 182, 200, 439
Bst6I CTCTTC 2 cut(s) 155, 495
BstC8I GCNNGC 2 cut(s) 69, 462
BstDEI CTNAG 1 cut(s) 510
BstDSI CCRYGG 1 cut(s) 400
BstFNI CGCG 1 cut(s) 402
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 6 cut(s) 54, 117, 324, 337, 382, 517
BstMAI GTCTC 2 cut(s) 237, 497
BstMBI GATC 6 cut(s) 51, 114, 321, 334, 379, 514
BstMWI GCNNNNNNNGC 3 cut(s) 11, 257, 314
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 246
BstUI CGCG 1 cut(s) 402
BstV1I GCAGC 4 cut(s) 23, 93, 383, 385
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 2 cut(s) 379, 514
BstYI RGATCY 2 cut(s) 379, 514
Bsu36I CCTNAGG 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 400
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 69, 462
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 410
Cfr42I CCGCGG 1 cut(s) 403
CseI GACGC 2 cut(s) 198, 442
Csp6I GTAC 1 cut(s) 26
CviAII CATG 6 cut(s) 158, 193, 293, 318, 356, 461
CviJI RGCY 9 cut(s) 60, 84, 92, 138, 145, 231, 260, 376, 396
CviKI_1 RGCY 9 cut(s) 60, 84, 92, 138, 145, 231, 260, 376, 396
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 1 cut(s) 510
DpnI GATC 6 cut(s) 53, 116, 323, 336, 381, 516
DpnII GATC 6 cut(s) 51, 114, 321, 334, 379, 514
DraI TTTAAA 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 155, 495
EarI CTCTTC 2 cut(s) 155, 495
Eco24I GRGCYC 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 410
Eco81I CCTNAGG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 262
FaeI CATG 6 cut(s) 161, 196, 296, 321, 359, 464
FaiI YATR 9 cut(s) 77, 159, 194, 207, 240, 294, 319, 357, 462
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 448
FatI CATG 6 cut(s) 157, 192, 292, 317, 355, 460
FauI CCCGC 1 cut(s) 72
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 6 cut(s) 12, 82, 85, 374, 397, 400
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 6 cut(s) 12, 82, 85, 374, 397, 400
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 491
GluI GCNGC 6 cut(s) 12, 82, 85, 374, 397, 400
GsuI CTGGAG 1 cut(s) 290
HapII CCGG 2 cut(s) 135, 215
HgaI GACGC 2 cut(s) 198, 442
HhaI GCGC 1 cut(s) 492
Hin1II CATG 6 cut(s) 161, 196, 296, 321, 359, 464
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 2 cut(s) 143, 229
HinfI GANTC 3 cut(s) 19, 44, 103
HpaII CCGG 2 cut(s) 135, 215
HphI GGTGA 3 cut(s) 204, 344, 373
Hpy166II GTNNAC 3 cut(s) 40, 48, 102
Hpy188III TCNNGA 5 cut(s) 107, 170, 325, 418, 512
Hpy8I GTNNAC 3 cut(s) 40, 48, 102
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 3 cut(s) 182, 200, 439
HpyCH4V TGCA 4 cut(s) 11, 163, 296, 373
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 257, 314
HpyF3I CTNAG 1 cut(s) 510
Hsp92II CATG 6 cut(s) 161, 196, 296, 321, 359, 464
HspAI GCGC 1 cut(s) 490
KspI CCGCGG 1 cut(s) 403
Kzo9I GATC 6 cut(s) 51, 114, 321, 334, 379, 514
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 4 cut(s) 23, 93, 383, 385
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 210
MalI GATC 6 cut(s) 53, 116, 323, 336, 381, 516
MbiI CCGCTC 1 cut(s) 123
MboI GATC 6 cut(s) 51, 114, 321, 334, 379, 514
MboII GAAGA 4 cut(s) 142, 188, 374, 512
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 2 cut(s) 379, 514
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 2 cut(s) 297, 446
MlyI GAGTC 3 cut(s) 13, 53, 97
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 82, 142, 164, 211, 248, 352, 414, 519
MseI TTAA 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 2 cut(s) 135, 215
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 402
MwoI GCNNNNNNNGC 3 cut(s) 11, 257, 314
NdeII GATC 6 cut(s) 51, 114, 321, 334, 379, 514
NlaIII CATG 6 cut(s) 161, 196, 296, 321, 359, 464
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 210
NspI RCATGY 1 cut(s) 464
PaeI GCATGC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 186
PctI GAATGC 1 cut(s) 473
PkrI GCNGC 6 cut(s) 13, 83, 86, 375, 398, 401
PleI GAGTC 3 cut(s) 13, 52, 97
PpsI GAGTC 3 cut(s) 13, 52, 97
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 516
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 2 cut(s) 379, 514
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
SacII CCGCGG 1 cut(s) 403
SalI GTCGAC 1 cut(s) 46
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 6 cut(s) 12, 82, 85, 374, 397, 400
Sau3AI GATC 6 cut(s) 51, 114, 321, 334, 379, 514
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 3 cut(s) 13, 53, 97
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 1 cut(s) 262
SetI ASST 6 cut(s) 134, 147, 233, 334, 363, 511
Sfr303I CCGCGG 1 cut(s) 403
SgrBI CCGCGG 1 cut(s) 403
SinI GGWCC 1 cut(s) 410
SphI GCATGC 1 cut(s) 464
Sse9I AATT 2 cut(s) 297, 446
SsiI CCGC 6 cut(s) 5, 65, 85, 121, 400, 402
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 182, 200, 439
TaqI TCGA 4 cut(s) 47, 96, 108, 522
TasI AATT 2 cut(s) 297, 446
TauI GCSGC 2 cut(s) 87, 402
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 1 cut(s) 210
TseI GCWGC 4 cut(s) 11, 81, 373, 396
Tsp45I GTSAC 1 cut(s) 210
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.