Rroxscaffold_3G00248810

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000953
Physical Location & Seq
Forward (+)
42191644 .. 42205042
13399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248810.1

Sequence Viewer

Length: 2067 bp
ATGGGAATTCTGCAGTTGCTGCTACCTTTGTTTTTCTGCTTCATTGTTCTTGTACCCAGCAAAGCAGCTGATCATGACTGTCCAGTTTCAAAGTGTAGTCCTGATGGTCCCCTTATCAAGTTTCCATTCCGTCTAAAGCATCAACCCCTTCACTGTGGCAATCCAGAGTTTGAGGTTTCCTGCAGCAGCTTCAGCAATATGAGCATGATTCAGCTCTCCACATCTTCAGGACTCTTCACCATCCAAACAATTGATTATGAAAACCAATACTTCAACGTCTTCGATGCAGATGGCTGCCTTCCAGGACGGCTTCCCGAGCTAGCCGAGCCTTCGCTACAGCCCGCGCGCCCGTGCGCTTGCAACCTTGCACAGCAGCCCCTGCTGCCCCCTCCCTGCAGCCCTGCGTTCCAGCCTGCTGCCACCGAAGCATCCCTGCTGCGCAAACAAGCCAACGCACACCCACTGCATCTTTTTCCCGTTCCTGTGCACATCCGTCTTCTTTCAAGCCTCGAAACGTCTAGTTCGGAAGCTCAGATCGAAAAATTTTCTTCATCAAATTTGATTGGACCAATCTATCTTCCACTACGAAGAGTGGATGGTAATCTGCACAACTGTGTACCGGATAGTGTATTAATTCTGAAATGGGACTTGGGCAATTTCCCAGGATGTCAAAACTGTAAAGCGGGAAGGTGCCAATTCAATTATAACAGCAATCAATTGGAGTGTCATCCTGAACTCAGCCCTCCACCTCCACCTCCATCTCCACCTCCACCTTCACCTGGTGTATCTAACTCAAAAAGGCCCAGGTATATCATAGGAGTGAGCATAGCTTGTTTTGTTCTCATCTCAGTAGCATCGGTAGCAATTTTCTTCCATGTCACGCAAACAAAAAGGATAGAGGAAAAGGAGAATCAAATGAAAATTGAAAGGTTCCTAAATGATCACAAGTCTCATGTACCCACTAGATACTCCAGTGCTAGTATAAAGAAGATGACAGATGGCTATAAGAAAAAGTTAGGTGAAGGAGGTTTTGGAAGTGTTTTCTTGGGAAAGCTACCCAACGGTGTTCAAGTCGCCATAAAAGTCCTCAATGGTTCTAAAAGAAGTGGAGAAGATTTTATCAATGAAGTGGGAACCATTGGTAGAATTCACCATGTTAATGTGGTTCGCTTACTTGGGTTCTCTGCTGAAGGATGCAAGCGTGCACTCATTTATGAGTTCATGCCAAACAAGTCCTTAGAAAAGTTCATCACATCTAGAGAATCTAATAATAATGACACTACATATGATTGGAAGAAACTTCAGCACATTATCACGGGTATAGGAAAGGGGATTGAATACCTTCACCAAGGGTGTGATCAGAGGATCCTCCACTTTGATATCAAGCCTCAGAATATCTTGTTAGACCATGACTTCAACCCTAAGATCTCTGATTTTGGTCTTGCAAAACTCTGTTCCAAGGAAGAAAGTATTATATCAATGACTGCTGCTAGAGGCACAGTAGGCTACATCGCACCCGAAGTGTTCATTAGGAACTTCGCAAACGTGTCCTACAAATCAGATGTATATAGTTTTGGGATGCTAGTGCTTGAAATGATAGGAGCTAGAAGGGAACCTCCTCTTGGAACTATAAACAGTGAGGCGTATTTTCCTGAATGGATTTATAAGCGTCTAATTCAGGGAGGAACATTGGATTTGAAGCTAGATACTGATGAAGATGCTCATGTTGCAAAGAAAATAGTGATTGTTGCACTATGGTGCATCCAGTGGCACCCGGTGAATCGCCCATCCATGAAATCTGTTGTTAGAATGCTAGAAGGAGATTCTGAAAGCTTGATCATGCCACCAAATCCATTCACCTTCACAAGTACTCAGACAATACGAAGCCCACCTCTCCTTCCTCCCCTTCCTACCATATCTCCCCTTCCTGTCTCTCTTTCTATCCCTCCGAAACTGTTCCATCTACTCCCACCGGCGTATGACACACGCGTCGCTCATGGAGAAGATCTACTCCCACCGTCGCTCGTCACCGTCTTCCGACGATACTCACCGCCGGTGGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

688

Amino Acids

76.45

Weight (kDa)

8.29

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 94 5.6e-13 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 336 - 603 5.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 336 - 605 1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 705, 1665
AasI GACNNNNNNGTC 1 cut(s) 1988
Acc16I TGCGCA 1 cut(s) 440
AccB1I GGYRCC 2 cut(s) 690, 1770
AccII CGCG 3 cut(s) 344, 346, 1989
AciI CCGC 3 cut(s) 342, 683, 2051
AclWI GGATC 2 cut(s) 1360, 1373
AcsI RAATTY 4 cut(s) 6, 542, 556, 1146
AcuI CTGAAG 4 cut(s) 175, 210, 1209, 1286
AdeI CACNNNGTG 2 cut(s) 782, 1777
AfaI GTAC 4 cut(s) 54, 618, 957, 1870
AfiI CCNNNNNNNGG 2 cut(s) 779, 1622
AflIII ACRYGT 2 cut(s) 1545, 1987
AjnI CCWGG 4 cut(s) 301, 661, 778, 803
AjuI GAANNNNNNNTTGG 6 cut(s) 632, 664, 1014, 1046, 1605, 1637
AleI CACNNNNGTG 2 cut(s) 612, 1756
Alw21I GWGCWC 2 cut(s) 489, 1207
Alw26I GTCTC 2 cut(s) 954, 1936
Alw44I GTGCAC 2 cut(s) 485, 1203
AlwI GGATC 2 cut(s) 1360, 1373
AlwNI CAGNNNCTG 2 cut(s) 19, 379
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 1 cut(s) 800
ApaLI GTGCAC 2 cut(s) 485, 1203
ApoI RAATTY 4 cut(s) 6, 542, 556, 1146
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 2 cut(s) 1341, 1955
AspLEI GCGC 4 cut(s) 346, 348, 356, 441
AspS9I GGNCC 3 cut(s) 107, 566, 801
AsuC2I CCSGG 1 cut(s) 1775
AsuHPI GGTGA 9 cut(s) 229, 768, 1031, 1142, 1337, 1789, 1849, 2020, 2040
AsuNHI GCTAGC 1 cut(s) 319
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 2 cut(s) 107, 566
BaeGI GKGCMC 2 cut(s) 489, 1207
BaeI ACNNNNGTAYC 2 cut(s) 958, 991
BamHI GGATCC 1 cut(s) 1365
BanI GGYRCC 2 cut(s) 690, 1770
BbsI GAAGAC 3 cut(s) 271, 488, 2026
Bbv12I GWGCWC 2 cut(s) 489, 1207
BccI CCATC 8 cut(s) 98, 248, 284, 590, 766, 992, 1795, 1968
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 4 cut(s) 303, 663, 780, 805
BclI TGATCA 4 cut(s) 70, 940, 1357, 1836
BcnI CCSGG 1 cut(s) 1775
BcoDI GTCTC 2 cut(s) 954, 1936
BfmI CTRYAG 4 cut(s) 11, 181, 335, 394
BglII AGATCT 2 cut(s) 1425, 2005
BmcAI AGTACT 1 cut(s) 1870
Bme1390I CCNGG 5 cut(s) 303, 663, 780, 805, 1775
Bme18I GGWCC 2 cut(s) 107, 566
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 3 cut(s) 107, 566, 801
BmiI GGNNCC 7 cut(s) 109, 692, 932, 1135, 1367, 1614, 1772
BmrFI CCNGG 5 cut(s) 303, 663, 780, 805, 1775
BmsI GCATC 9 cut(s) 148, 274, 437, 475, 863, 1184, 1569, 1708, 1770
BmtI GCTAGC 1 cut(s) 323
BpiI GAAGAC 3 cut(s) 271, 488, 2026
BpmI CTGGAG 1 cut(s) 955
BpuMI CCSGG 1 cut(s) 1775
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 661, 803, 1348, 1458
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 1903, 1933
Bsc4I CCNNNNNNNGG 2 cut(s) 779, 1622
Bse118I RCCGGY 2 cut(s) 1972, 2053
Bse1I ACTGG 3 cut(s) 83, 972, 1765
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 4 cut(s) 303, 663, 780, 805
BseDI CCNNGG 4 cut(s) 661, 803, 1348, 1458
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 2 cut(s) 779, 1622
BseMII CTCAG 5 cut(s) 545, 751, 861, 1403, 1886
BseNI ACTGG 3 cut(s) 83, 972, 1765
BsePI GCGCGC 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 1608
BseSI GKGCMC 2 cut(s) 489, 1207
BseYI CCCAGC 1 cut(s) 56
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 3 cut(s) 344, 346, 1989
BshFI GGCC 1 cut(s) 802
BshNI GGYRCC 2 cut(s) 690, 1770
BsiHKAI GWGCWC 2 cut(s) 489, 1207
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 4 cut(s) 620, 1775, 1973, 2054
BslFI GGGAC 2 cut(s) 93, 659
BslI CCNNNNNNNGG 2 cut(s) 779, 1622
BsmAI GTCTC 2 cut(s) 954, 1936
BsmFI GGGAC 2 cut(s) 93, 659
BsmI GAATGC 1 cut(s) 1815
BsnI GGCC 1 cut(s) 802
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 489, 1207
Bsp143I GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
BspACI CCGC 3 cut(s) 342, 683, 2051
BspANI GGCC 1 cut(s) 802
BspCNI CTCAG 5 cut(s) 544, 750, 860, 1402, 1885
BspFNI CGCG 3 cut(s) 344, 346, 1989
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 7 cut(s) 109, 692, 932, 1135, 1367, 1614, 1772
BspMAI CTGCAG 3 cut(s) 15, 185, 398
BspOI GCTAGC 1 cut(s) 323
BspPI GGATC 2 cut(s) 1360, 1373
BspT107I GGYRCC 2 cut(s) 690, 1770
BsrFI RCCGGY 2 cut(s) 1972, 2053
BsrI ACTGG 3 cut(s) 83, 972, 1765
BssAI RCCGGY 2 cut(s) 1972, 2053
BssECI CCNNGG 4 cut(s) 661, 803, 1348, 1458
BssHII GCGCGC 1 cut(s) 344
BssMI GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
BssT1I CCWWGG 2 cut(s) 1348, 1458
Bst2UI CCWGG 4 cut(s) 303, 663, 780, 805
Bst6I CTCTTC 2 cut(s) 239, 583
BstAPI GCANNNNNTGC 2 cut(s) 19, 379
BstC8I GCNNGC 7 cut(s) 321, 342, 346, 358, 414, 1199, 1203
BstDEI CTNAG 7 cut(s) 531, 737, 847, 1237, 1389, 1422, 1872
BstFNI CGCG 3 cut(s) 344, 346, 1989
BstHHI GCGC 4 cut(s) 346, 348, 356, 441
BstKTI GATC 8 cut(s) 73, 537, 943, 1360, 1368, 1428, 1839, 2008
BstMAI GTCTC 2 cut(s) 954, 1936
BstMBI GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
BstNI CCWGG 4 cut(s) 303, 663, 780, 805
BstSCI CCNGG 5 cut(s) 301, 661, 778, 803, 1773
BstSFI CTRYAG 4 cut(s) 11, 181, 335, 394
BstSLI GKGCMC 2 cut(s) 489, 1207
BstUI CGCG 3 cut(s) 344, 346, 1989
BstV2I GAAGAC 3 cut(s) 271, 488, 2026
BstX2I RGATCY 3 cut(s) 1365, 1425, 2005
BstYI RGATCY 3 cut(s) 1365, 1425, 2005
BsuRI GGCC 1 cut(s) 802
BtgZI GCGATG 1 cut(s) 1495
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 5 cut(s) 151, 461, 979, 1642, 1772
Cac8I GCNNGC 7 cut(s) 321, 342, 346, 358, 414, 1199, 1203
CaiI CAGNNNCTG 2 cut(s) 19, 379
CciI TCATGA 1 cut(s) 73
CfoI GCGC 4 cut(s) 346, 348, 356, 441
Cfr10I RCCGGY 2 cut(s) 1972, 2053
Cfr13I GGNCC 3 cut(s) 107, 566, 801
CseI GACGC 2 cut(s) 1658, 1978
CsiI ACCWGGT 1 cut(s) 778
Csp6I GTAC 4 cut(s) 53, 617, 956, 1869
CviQI GTAC 4 cut(s) 53, 617, 956, 1869
DdeI CTNAG 7 cut(s) 531, 737, 847, 1237, 1389, 1422, 1872
DpnI GATC 8 cut(s) 72, 536, 942, 1359, 1367, 1427, 1838, 2007
DpnII GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
DraIII CACNNNGTG 2 cut(s) 782, 1777
DrdI GACNNNNNNGTC 1 cut(s) 1988
DseDI GACNNNNNNGTC 1 cut(s) 1988
Eam1104I CTCTTC 2 cut(s) 239, 583
EarI CTCTTC 2 cut(s) 239, 583
Eco130I CCWWGG 2 cut(s) 1348, 1458
Eco32I GATATC 1 cut(s) 1381
Eco47I GGWCC 2 cut(s) 107, 566
Eco57I CTGAAG 4 cut(s) 175, 210, 1209, 1286
Eco88I CYCGRG 1 cut(s) 314
EcoRI GAATTC 2 cut(s) 6, 1146
EcoRII CCWGG 4 cut(s) 301, 661, 778, 803
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 2 cut(s) 1348, 1458
ErhI CCWWGG 2 cut(s) 1348, 1458
FaqI GGGAC 2 cut(s) 93, 659
FauI CCCGC 2 cut(s) 349, 676
FauNDI CATATG 1 cut(s) 1285
FbaI TGATCA 4 cut(s) 70, 940, 1357, 1836
FspI TGCGCA 1 cut(s) 440
GlaI GCGC 4 cut(s) 345, 347, 355, 440
GsaI CCCAGC 1 cut(s) 60
GsuI CTGGAG 1 cut(s) 955
HaeIII GGCC 1 cut(s) 802
HapII CCGG 4 cut(s) 620, 1775, 1973, 2054
HgaI GACGC 2 cut(s) 1658, 1978
HhaI GCGC 4 cut(s) 346, 348, 356, 441
Hin6I GCGC 4 cut(s) 344, 346, 354, 439
HinP1I GCGC 4 cut(s) 344, 346, 354, 439
HindIII AAGCTT 1 cut(s) 1831
HinfI GANTC 6 cut(s) 208, 231, 910, 1262, 1780, 1823
HpaII CCGG 4 cut(s) 620, 1775, 1973, 2054
HphI GGTGA 9 cut(s) 229, 768, 1031, 1142, 1337, 1789, 1849, 2020, 2040
Hpy166II GTNNAC 3 cut(s) 487, 617, 1205
Hpy188III TCNNGA 8 cut(s) 74, 101, 164, 228, 314, 731, 1257, 1652
Hpy8I GTNNAC 3 cut(s) 487, 617, 1205
Hpy99I CGWCG 3 cut(s) 1994, 2023, 2043
HpyCH4IV ACGT 3 cut(s) 276, 515, 1545
HpyF3I CTNAG 7 cut(s) 531, 737, 847, 1237, 1389, 1422, 1872
HpySE526I ACGT 3 cut(s) 276, 515, 1545
HspAI GCGC 4 cut(s) 344, 346, 354, 439
Ksp22I TGATCA 4 cut(s) 70, 940, 1357, 1836
Kzo9I GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
LmnI GCTCC 1 cut(s) 1601
LweI GCATC 9 cut(s) 148, 274, 437, 475, 863, 1184, 1569, 1708, 1770
MabI ACCWGGT 1 cut(s) 778
MaeII ACGT 3 cut(s) 276, 515, 1545
MaeIII GTNAC 2 cut(s) 877, 2026
MalI GATC 8 cut(s) 72, 536, 942, 1359, 1367, 1427, 1838, 2007
MboI GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
MfeI CAATTG 2 cut(s) 249, 716
MflI RGATCY 3 cut(s) 1365, 1425, 2005
MhlI GDGCHC 2 cut(s) 489, 1207
MluI ACGCGT 1 cut(s) 1987
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 1 cut(s) 2062
MroXI GAANNNNTTC 2 cut(s) 1341, 1955
MseI TTAA 2 cut(s) 632, 1158
MslI CAYNNNNRTG 4 cut(s) 612, 818, 1158, 1756
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 4 cut(s) 620, 1775, 1973, 2054
MspR9I CCNGG 5 cut(s) 303, 663, 780, 805, 1775
MunI CAATTG 2 cut(s) 249, 716
Mva1269I GAATGC 1 cut(s) 1815
MvaI CCWGG 4 cut(s) 303, 663, 780, 805
MvnI CGCG 3 cut(s) 344, 346, 1989
NciI CCSGG 1 cut(s) 1775
NdeI CATATG 1 cut(s) 1285
NdeII GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
NheI GCTAGC 1 cut(s) 319
NlaIV GGNNCC 7 cut(s) 109, 692, 932, 1135, 1367, 1614, 1772
NmeAIII GCCGAG 1 cut(s) 349
NmuCI GTSAC 2 cut(s) 877, 2026
NsbI TGCGCA 1 cut(s) 440
OliI CACNNNNGTG 2 cut(s) 612, 1756
PagI TCATGA 1 cut(s) 73
PauI GCGCGC 1 cut(s) 344
PctI GAATGC 1 cut(s) 1815
PdmI GAANNNNTTC 2 cut(s) 1341, 1955
PfeI GAWTC 5 cut(s) 208, 910, 1262, 1780, 1823
PfoI TCCNGGA 1 cut(s) 301
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PshBI ATTAAT 1 cut(s) 632
PsiI TTATAA 2 cut(s) 705, 1665
Psp6I CCWGG 4 cut(s) 301, 661, 778, 803
PspFI CCCAGC 1 cut(s) 56
PspGI CCWGG 4 cut(s) 301, 661, 778, 803
PspN4I GGNNCC 7 cut(s) 109, 692, 932, 1135, 1367, 1614, 1772
PspPI GGNCC 3 cut(s) 107, 566, 801
PstI CTGCAG 3 cut(s) 15, 185, 398
PstNI CAGNNNCTG 2 cut(s) 19, 379
PsuI RGATCY 3 cut(s) 1365, 1425, 2005
PteI GCGCGC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 4 cut(s) 54, 618, 957, 1870
RsaNI GTAC 4 cut(s) 53, 617, 956, 1869
RseI CAYNNNNRTG 4 cut(s) 612, 818, 1158, 1756
SaqAI TTAA 2 cut(s) 632, 1158
Sau3AI GATC 8 cut(s) 70, 534, 940, 1357, 1365, 1425, 1836, 2005
Sau96I GGNCC 3 cut(s) 107, 566, 801
ScaI AGTACT 1 cut(s) 1870
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 5 cut(s) 303, 663, 780, 805, 1775
SduI GDGCHC 2 cut(s) 489, 1207
SexAI ACCWGGT 1 cut(s) 778
SfaNI GCATC 9 cut(s) 148, 274, 437, 475, 863, 1184, 1569, 1708, 1770
SfcI CTRYAG 4 cut(s) 11, 181, 335, 394
SgrAI CRCCGGYG 2 cut(s) 1972, 2053
SinI GGWCC 2 cut(s) 107, 566
SmiMI CAYNNNNRTG 4 cut(s) 612, 818, 1158, 1756
SsiI CCGC 3 cut(s) 342, 683, 2051
StyD4I CCNGG 5 cut(s) 301, 661, 778, 803, 1773
StyI CCWWGG 2 cut(s) 1348, 1458
TaiI ACGT 3 cut(s) 279, 518, 1548
TaqI TCGA 3 cut(s) 282, 510, 537
TatI WGTACW 1 cut(s) 1868
TfiI GAWTC 5 cut(s) 208, 910, 1262, 1780, 1823
Tru1I TTAA 2 cut(s) 632, 1158
Tru9I TTAA 2 cut(s) 632, 1158
TscAI CASTG 5 cut(s) 158, 468, 979, 1642, 1772
TseFI GTSAC 2 cut(s) 877, 2026
Tsp45I GTSAC 2 cut(s) 877, 2026
TspGWI ACGGA 2 cut(s) 119, 482
TspRI CASTG 5 cut(s) 158, 468, 979, 1642, 1772
VneI GTGCAC 2 cut(s) 485, 1203
VpaK11BI GGWCC 2 cut(s) 107, 566
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 4 cut(s) 6, 542, 556, 1146
XbaI TCTAGA 1 cut(s) 1256
XmnI GAANNNNTTC 2 cut(s) 1341, 1955
ZrmI AGTACT 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.