pycom04g20720

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000953
Physical Location & Seq
Forward (+)
22136078 .. 22138136
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20720.2

Sequence Viewer

Length: 1944 bp
ATGAATATCGATAGCCAACTCCTCCAACCTCTGTTTTTCTTGCTTGTAATTTTCTTCGCCACGGCCATTAATGTTGTACCAAGCAAAGCAGCAGCCGATGATCATGGCGCTGACTGCCCAATTTCACAGTGTAGGGATGATGGTCCCGTTATCAAGTTTCCCTTTCGCCTAAAAGATGATCCCCCACACTGTGGCCATCCACAGTTTGAGGTTTCTTGCTTGCAAAACAAGACAATGATTCAGCTCTCATCGTCATCGGGACTGTTTCCCATACGAACAATAGATTACAAACGGAAACAGTTTCGCGTCTACGATGCAAATGGTTGCCTCCCCAGACGCCTTCTTAACTTTACCATCTCCAGCAACTCATCTCAGCTCTTTCATATCTCCCATGATTACGGGTCCTCTGATTACCTGTGTTTCGACTGGGATTCATTAACTAATCCTCTGATAGTAAACCTTACATTATTAAACTGTTCCACAGATCAAAACTTGACCAGAACCTTCAATGCGAACTACTTTCCTATCAGCTGTCTCAGTGTCCCAGGGCACCAAGTTCTGGCTTTTCCACCCCATGCTTCTGTCGCTGAATTGCCTGTGCCAACTTGTAGCATTTGGAGAAAACTCCAATATCTTCCGTTACGAAGCGTGGATGCTAATAACAACGGATACGAGTGTGACTTGGACAGTTTTCTGTGGCTGGAATGGGAACCATGGGCTAGTTTCCCATCATGCCAATACTGTGGAGGATATTGCGAGCTCAATAGCAGCAATCAGTTGGGGTGCTATTTTCAGGATCCTCTACAACAGCAAAAAGGCCATGCATCAAGTAGGAGGTCGATTATCTTGGGAGCGAGCGTAGCTTCAATTGCTCTTATATCAGCGGTGGCAGCAGTAGTATTTTTTTACCATGTAAAACGATCAAAGAGCGTACAGGAGAAAGAGAATCAACTCAAAGTTGAAAGGTTCCTGAAGGATCACAAATCTCACGTACCAACGAGATACTCCTACGCCGATATTAAAAAGATGACAAATGGATTCAAGAAAAAGTTAGGTGAAGGAGGTTTCGGAAGCGTTTACAGTGGAGAGCTTCCAATTAATGGAGTTCCAGTCGCCGTAAAAGTCCTCAGTGACTCGAAAGGAAATGGAGAAGATTTTGTTAATGAAGTGGGAACCATTGGCAGAATTCATCATGTCAATGTGGTTCGTCTACTAGGTTTTTCTGCCGAAGTAGGCAAACGTGCGCTTATTTACGAGCTCATGCCAAACAGGTCCCTAGAGAACTTCATCACATCTAAAGATCAATCCAATAACACTTTGTTTGATTGGCAGAAACTTCATAACATCGTCAATGGTATAGCGAAGGGAATCGAGTATCTTCACCAAGGGTGCGACCGCATGATCCTCCACTTTGATATCAAGCCTCGTAACATACTGTTAGACCATGATTTCAGTCCTAAGATCTCGGATTTTGGTCTGGCTAAACTCTGTTCCAAAGAAGACAGTATCGTATCTATGACAGCTGCTAGAGGCACCGTGGGCTACATTGCACCAGAAGTGTTTAACGGGAACTTTGGGAACGTGTCACACAAGTCAGATGTGTACAGTTTCGGGATGCTGGTGCTTGAGATTGTTGGAGCTAGGAAGGAAACTGCTCTTGCTTCTGGCAACAATGAGGTCTACTTTCCAGAGTTGGTCTACAATCGTCTCGTTCAAGGAGAAGAGTTGGATTTGAAGCTAGATACCGATGAGGACACTCAGATTGCTAAGAAACTAGTGATTGTGGCACTTTGGTGCATCCAGTGGTACCCGGTGAATCGCCCTCCCATGAAAGCAGTTGTTAGAATGCTAGAAGGAGGCTCTGAAAACTTGAGCATGCCACCAAATCCATTTGCTTCCACAAGTGCACAGACAGATCAACCGAGAACAACACTGTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

648

Amino Acids

72.25

Weight (kDa)

6.65

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1806
AccB1I GGYRCC 3 cut(s) 549, 1532, 1806
AccB7I CCANNNNNTGG 3 cut(s) 191, 559, 1100
AccI GTMKAC 4 cut(s) 309, 1210, 1680, 1698
AccII CGCG 1 cut(s) 306
AciI CCGC 2 cut(s) 884, 1396
AclWI GGATC 5 cut(s) 173, 791, 804, 984, 1396
AcoI YGGCCR 2 cut(s) 63, 193
AcsI RAATTY 1 cut(s) 1185
AcuI CTGAAG 1 cut(s) 992
AcyI GRCGYC 1 cut(s) 337
AdeI CACNNNGTG 1 cut(s) 191
AfaI GTAC 5 cut(s) 78, 933, 993, 1604, 1808
AfiI CCNNNNNNNGG 3 cut(s) 191, 559, 1100
AflIII ACRYGT 1 cut(s) 1581
AgsI TTSAA 6 cut(s) 508, 867, 962, 1042, 1715, 1735
AhlI ACTAGT 1 cut(s) 1774
AjnI CCWGG 1 cut(s) 544
AleI CACNNNNGTG 1 cut(s) 1792
Alw21I GWGCWC 3 cut(s) 762, 1260, 1909
Alw26I GTCTC 2 cut(s) 539, 1712
Alw44I GTGCAC 1 cut(s) 1905
AlwI GGATC 5 cut(s) 173, 791, 804, 984, 1396
AoxI GGCC 3 cut(s) 63, 193, 817
ApaLI GTGCAC 1 cut(s) 1905
ApeKI GCWGC 5 cut(s) 89, 92, 768, 890, 1523
ApoI RAATTY 1 cut(s) 1185
AseI ATTAAT 2 cut(s) 69, 1098
Asp718I GGTACC 1 cut(s) 1806
AspLEI GCGC 2 cut(s) 110, 1246
AspS9I GGNCC 3 cut(s) 143, 402, 1272
AsuC2I CCSGG 1 cut(s) 1811
AsuHPI GGTGA 3 cut(s) 1067, 1373, 1825
AvaII GGWCC 3 cut(s) 143, 402, 1272
BaeGI GKGCMC 2 cut(s) 552, 1909
BaeI ACNNNNGTAYC 2 cut(s) 661, 694
BalI TGGCCA 1 cut(s) 195
BamHI GGATCC 1 cut(s) 796
BanI GGYRCC 3 cut(s) 549, 1532, 1806
BanII GRGCYC 2 cut(s) 762, 1260
BbsI GAAGAC 1 cut(s) 1506
Bbv12I GWGCWC 3 cut(s) 762, 1260, 1909
BbvI GCAGC 5 cut(s) 101, 104, 780, 902, 1510
BccI CCATC 4 cut(s) 134, 204, 362, 736
BceAI ACGGC 2 cut(s) 78, 1100
BciT130I CCWGG 1 cut(s) 546
BciVI GTATCC 1 cut(s) 662
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 1 cut(s) 1811
BcoDI GTCTC 2 cut(s) 539, 1712
BcuI ACTAGT 1 cut(s) 1774
BfaI CTAG 8 cut(s) 720, 1214, 1277, 1527, 1641, 1739, 1775, 1850
BfoI RGCGCY 1 cut(s) 111
BfuI GTATCC 1 cut(s) 662
BglII AGATCT 1 cut(s) 1461
BisI GCNGC 5 cut(s) 90, 93, 769, 891, 1524
BlsI GCNGC 5 cut(s) 91, 94, 770, 892, 1525
Bme1390I CCNGG 2 cut(s) 546, 1811
Bme18I GGWCC 3 cut(s) 143, 402, 1272
BmgT120I GGNCC 3 cut(s) 143, 402, 1272
BmrFI CCNGG 2 cut(s) 546, 1811
BmrI ACTGGG 1 cut(s) 436
BmsI GCATC 5 cut(s) 304, 643, 833, 1605, 1806
BmuI ACTGGG 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 1506
BpmI CTGGAG 1 cut(s) 343
BpuEI CTTGAG 2 cut(s) 1646, 1891
BpuMI CCSGG 1 cut(s) 1811
Bsa29I ATCGAT 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 991
BsaHI GRCGYC 1 cut(s) 337
BsaJI CCNNGG 6 cut(s) 60, 544, 545, 713, 1384, 1536
BsaXI ACNNNNNCTCC 4 cut(s) 843, 873, 1095, 1125
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 559, 1100
Bse1I ACTGG 3 cut(s) 431, 1109, 1801
Bse3DI GCAATG 1 cut(s) 1545
BseBI CCWGG 1 cut(s) 546
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 6 cut(s) 60, 544, 545, 713, 1384, 1536
BseGI GGATG 5 cut(s) 142, 196, 658, 1620, 1797
BseLI CCNNNNNNNGG 3 cut(s) 191, 559, 1100
BseMI GCAATG 1 cut(s) 1545
BseMII CTCAG 4 cut(s) 386, 550, 1141, 1772
BseNI ACTGG 3 cut(s) 431, 1109, 1801
BseRI GAGGAG 1 cut(s) 11
BseSI GKGCMC 2 cut(s) 552, 1909
BseXI GCAGC 5 cut(s) 101, 104, 780, 902, 1510
Bsh1236I CGCG 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 1396
BshFI GGCC 3 cut(s) 65, 195, 819
BshNI GGYRCC 3 cut(s) 549, 1532, 1806
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 1396
BsiHKAI GWGCWC 3 cut(s) 762, 1260, 1909
BsiSI CCGG 1 cut(s) 1811
BslFI GGGAC 4 cut(s) 129, 273, 527, 1258
BslI CCNNNNNNNGG 3 cut(s) 191, 559, 1100
BsmAI GTCTC 2 cut(s) 539, 1712
BsmBI CGTCTC 1 cut(s) 1712
BsmFI GGGAC 4 cut(s) 129, 273, 527, 1258
BsmI GAATGC 1 cut(s) 1851
BsnI GGCC 3 cut(s) 65, 195, 819
Bsp1286I GDGCHC 4 cut(s) 552, 762, 1260, 1909
Bsp1407I TGTACA 1 cut(s) 1602
Bsp19I CCATGG 1 cut(s) 713
BspACI CCGC 2 cut(s) 884, 1396
BspANI GGCC 3 cut(s) 65, 195, 819
BspCNI CTCAG 4 cut(s) 385, 549, 1140, 1771
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 1 cut(s) 306
BspPI GGATC 5 cut(s) 173, 791, 804, 984, 1396
BspT107I GGYRCC 3 cut(s) 549, 1532, 1806
BsrDI GCAATG 1 cut(s) 1545
BsrGI TGTACA 1 cut(s) 1602
BsrI ACTGG 3 cut(s) 431, 1109, 1801
BssECI CCNNGG 6 cut(s) 60, 544, 545, 713, 1384, 1536
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 2 cut(s) 713, 1384
Bst2UI CCWGG 1 cut(s) 546
Bst6I CTCTTC 1 cut(s) 1716
BstACI GRCGYC 1 cut(s) 337
BstAUI TGTACA 1 cut(s) 1602
BstBAI YACGTR 1 cut(s) 991
BstC8I GCNNGC 4 cut(s) 221, 758, 856, 1877
BstDEI CTNAG 6 cut(s) 372, 536, 1127, 1458, 1758, 1767
BstDSI CCRYGG 3 cut(s) 60, 713, 1536
BstF5I GGATG 5 cut(s) 142, 196, 658, 1620, 1797
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 1246
BstMAI GTCTC 2 cut(s) 539, 1712
BstMCI CGRYCG 1 cut(s) 1396
BstMWI GCNNNNNNNGC 6 cut(s) 114, 584, 860, 869, 890, 1539
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 1879
BstSCI CCNGG 2 cut(s) 544, 1809
BstSLI GKGCMC 2 cut(s) 552, 1909
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 5 cut(s) 101, 104, 780, 902, 1510
BstV2I GAAGAC 1 cut(s) 1506
BstX2I RGATCY 2 cut(s) 796, 1461
BstXI CCANNNNNNTGG 1 cut(s) 743
BstYI RGATCY 2 cut(s) 796, 1461
Bsu15I ATCGAT 1 cut(s) 9
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 3 cut(s) 65, 195, 819
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 3 cut(s) 60, 713, 1536
BtsCI GGATG 5 cut(s) 142, 196, 658, 1620, 1797
BtsIMutI CAGTG 7 cut(s) 134, 187, 544, 1087, 1135, 1808, 1931
Cac8I GCNNGC 4 cut(s) 221, 758, 856, 1877
CfoI GCGC 2 cut(s) 110, 1246
Cfr13I GGNCC 3 cut(s) 143, 402, 1272
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 2 cut(s) 295, 345
Csp6I GTAC 5 cut(s) 77, 932, 992, 1603, 1807
CviQI GTAC 5 cut(s) 77, 932, 992, 1603, 1807
DdeI CTNAG 6 cut(s) 372, 536, 1127, 1458, 1758, 1767
DraIII CACNNNGTG 1 cut(s) 191
EaeI YGGCCR 2 cut(s) 63, 193
Eam1104I CTCTTC 1 cut(s) 1716
EarI CTCTTC 1 cut(s) 1716
Ecl136II GAGCTC 2 cut(s) 760, 1258
Eco130I CCWWGG 2 cut(s) 713, 1384
Eco24I GRGCYC 2 cut(s) 762, 1260
Eco32I GATATC 1 cut(s) 1417
Eco47I GGWCC 3 cut(s) 143, 402, 1272
Eco53kI GAGCTC 2 cut(s) 760, 1258
Eco57I CTGAAG 1 cut(s) 992
EcoICRI GAGCTC 2 cut(s) 760, 1258
EcoO109I RGGNCCY 2 cut(s) 402, 1272
EcoRI GAATTC 1 cut(s) 1185
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 1 cut(s) 1417
EcoT14I CCWWGG 2 cut(s) 713, 1384
EcoT22I ATGCAT 1 cut(s) 826
EcoT38I GRGCYC 2 cut(s) 762, 1260
ErhI CCWWGG 2 cut(s) 713, 1384
Esp3I CGTCTC 1 cut(s) 1712
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 4 cut(s) 129, 273, 527, 1258
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 4 cut(s) 309, 1210, 1680, 1698
Fnu4HI GCNGC 5 cut(s) 90, 93, 769, 891, 1524
FokI GGATG 5 cut(s) 149, 183, 665, 1627, 1784
FriOI GRGCYC 2 cut(s) 762, 1260
Fsp4HI GCNGC 5 cut(s) 90, 93, 769, 891, 1524
FspBI CTAG 8 cut(s) 720, 1214, 1277, 1527, 1641, 1739, 1775, 1850
GlaI GCGC 2 cut(s) 109, 1245
GluI GCNGC 5 cut(s) 90, 93, 769, 891, 1524
GsuI CTGGAG 1 cut(s) 343
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 3 cut(s) 65, 195, 819
HapII CCGG 1 cut(s) 1811
HgaI GACGC 2 cut(s) 295, 345
HhaI GCGC 2 cut(s) 110, 1246
Hin1I GRCGYC 1 cut(s) 337
Hin6I GCGC 2 cut(s) 108, 1244
HinP1I GCGC 2 cut(s) 108, 1244
HinfI GANTC 7 cut(s) 238, 431, 946, 1038, 1133, 1368, 1816
HpaII CCGG 1 cut(s) 1811
HphI GGTGA 3 cut(s) 1067, 1373, 1825
Hpy166II GTNNAC 8 cut(s) 310, 457, 1078, 1211, 1603, 1681, 1699, 1907
Hpy188I TCNGA 7 cut(s) 409, 450, 1070, 1468, 1597, 1761, 1864
Hpy188III TCNNGA 6 cut(s) 258, 794, 970, 1042, 1612, 1688
Hpy8I GTNNAC 8 cut(s) 310, 457, 1078, 1211, 1603, 1681, 1699, 1907
HpyAV CCTTC 7 cut(s) 350, 514, 967, 1052, 1357, 1639, 1847
HpyCH4IV ACGT 3 cut(s) 990, 1240, 1581
HpyCH4V TGCA 6 cut(s) 223, 317, 824, 1550, 1797, 1907
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 584, 860, 869, 890, 1539
HpyF3I CTNAG 6 cut(s) 372, 536, 1127, 1458, 1758, 1767
HpySE526I ACGT 3 cut(s) 990, 1240, 1581
Hsp92I GRCGYC 1 cut(s) 337
HspAI GCGC 2 cut(s) 108, 1244
KpnI GGTACC 1 cut(s) 1810
Ksp22I TGATCA 1 cut(s) 100
LmnI GCTCC 2 cut(s) 851, 1637
Lsp1109I GCAGC 5 cut(s) 101, 104, 780, 902, 1510
LweI GCATC 5 cut(s) 304, 643, 833, 1605, 1806
MaeI CTAG 8 cut(s) 720, 1214, 1277, 1527, 1641, 1739, 1775, 1850
MaeII ACGT 3 cut(s) 990, 1240, 1581
MaeIII GTNAC 5 cut(s) 639, 677, 1130, 1427, 1584
MboII GAAGA 6 cut(s) 46, 626, 1163, 1370, 1511, 1733
MfeI CAATTG 1 cut(s) 867
MflI RGATCY 2 cut(s) 796, 1461
MhlI GDGCHC 4 cut(s) 552, 762, 1260, 1909
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 6 cut(s) 48, 120, 590, 867, 1095, 1185
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 1 cut(s) 1127
MmeI TCCRAC 3 cut(s) 49, 1615, 1707
Mox20I TGGCCA 1 cut(s) 195
Mph1103I ATGCAT 1 cut(s) 826
MscI TGGCCA 1 cut(s) 195
MseI TTAA 9 cut(s) 69, 345, 437, 470, 1020, 1098, 1161, 1563, 1942
MslI CAYNNNNRTG 2 cut(s) 1197, 1792
Msp20I TGGCCA 1 cut(s) 195
MspA1I CMGCKG 3 cut(s) 531, 884, 1523
MspI CCGG 1 cut(s) 1811
MspR9I CCNGG 2 cut(s) 546, 1811
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 1 cut(s) 1851
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 6 cut(s) 114, 584, 860, 869, 890, 1539
NciI CCSGG 1 cut(s) 1811
NcoI CCATGG 1 cut(s) 713
NmuCI GTSAC 3 cut(s) 677, 1130, 1584
NsiI ATGCAT 1 cut(s) 826
NspI RCATGY 1 cut(s) 1879
OliI CACNNNNGTG 1 cut(s) 1792
PaeI GCATGC 1 cut(s) 1879
PasI CCCWGGG 1 cut(s) 545
PctI GAATGC 1 cut(s) 1851
PfeI GAWTC 6 cut(s) 238, 431, 946, 1038, 1368, 1816
PflMI CCANNNNNTGG 3 cut(s) 191, 559, 1100
PkrI GCNGC 5 cut(s) 91, 94, 770, 892, 1525
PleI GAGTC 1 cut(s) 1127
PpsI GAGTC 1 cut(s) 1127
Ppu21I YACGTR 1 cut(s) 991
PpuMI RGGWCCY 2 cut(s) 402, 1272
PshBI ATTAAT 2 cut(s) 69, 1098
Psp124BI GAGCTC 2 cut(s) 762, 1260
Psp5II RGGWCCY 2 cut(s) 402, 1272
Psp6I CCWGG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 544
PspPI GGNCC 3 cut(s) 143, 402, 1272
PspPPI RGGWCCY 2 cut(s) 402, 1272
PsuI RGATCY 2 cut(s) 796, 1461
PvuII CAGCTG 2 cut(s) 531, 1523
RsaI GTAC 5 cut(s) 78, 933, 993, 1604, 1808
RsaNI GTAC 5 cut(s) 77, 932, 992, 1603, 1807
RseI CAYNNNNRTG 2 cut(s) 1197, 1792
SacI GAGCTC 2 cut(s) 762, 1260
SaqAI TTAA 9 cut(s) 69, 345, 437, 470, 1020, 1098, 1161, 1563, 1942
SatI GCNGC 5 cut(s) 90, 93, 769, 891, 1524
Sau96I GGNCC 3 cut(s) 143, 402, 1272
SchI GAGTC 1 cut(s) 1127
ScrFI CCNGG 2 cut(s) 546, 1811
SduI GDGCHC 4 cut(s) 552, 762, 1260, 1909
SfaNI GCATC 5 cut(s) 304, 643, 833, 1605, 1806
SinI GGWCC 3 cut(s) 143, 402, 1272
SmiMI CAYNNNNRTG 2 cut(s) 1197, 1792
SmlI CTYRAG 2 cut(s) 1625, 1870
SmoI CTYRAG 2 cut(s) 1625, 1870
SpeI ACTAGT 1 cut(s) 1774
SphI GCATGC 1 cut(s) 1879
Sse9I AATT 6 cut(s) 48, 120, 590, 867, 1095, 1185
SsiI CCGC 2 cut(s) 884, 1396
SspMI CTAG 8 cut(s) 720, 1214, 1277, 1527, 1641, 1739, 1775, 1850
SstI GAGCTC 2 cut(s) 762, 1260
StyD4I CCNGG 2 cut(s) 544, 1809
StyI CCWWGG 2 cut(s) 713, 1384
TaiI ACGT 3 cut(s) 993, 1243, 1584
TaqI TCGA 5 cut(s) 9, 423, 839, 1136, 1371
TasI AATT 6 cut(s) 48, 120, 590, 867, 1095, 1185
TatI WGTACW 1 cut(s) 1602
TfiI GAWTC 6 cut(s) 238, 431, 946, 1038, 1368, 1816
Tru1I TTAA 9 cut(s) 69, 345, 437, 470, 1020, 1098, 1161, 1563, 1942
Tru9I TTAA 9 cut(s) 69, 345, 437, 470, 1020, 1098, 1161, 1563, 1942
TscAI CASTG 7 cut(s) 134, 194, 544, 1087, 1135, 1808, 1938
TseFI GTSAC 3 cut(s) 677, 1130, 1584
TseI GCWGC 5 cut(s) 89, 92, 768, 890, 1523
Tsp45I GTSAC 3 cut(s) 677, 1130, 1584
TspDTI ATGAA 8 cut(s) 17, 371, 423, 1178, 1179, 1276, 1328, 1844
TspGWI ACGGA 3 cut(s) 307, 627, 681
TspRI CASTG 7 cut(s) 134, 194, 544, 1087, 1135, 1808, 1938
Van91I CCANNNNNTGG 3 cut(s) 191, 559, 1100
VneI GTGCAC 1 cut(s) 1905
VpaK11BI GGWCC 3 cut(s) 143, 402, 1272
VspI ATTAAT 2 cut(s) 69, 1098
XapI RAATTY 1 cut(s) 1185
XceI RCATGY 1 cut(s) 1879
XmiI GTMKAC 4 cut(s) 309, 1210, 1680, 1698
XspI CTAG 8 cut(s) 720, 1214, 1277, 1527, 1641, 1739, 1775, 1850
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.