Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001101

Physical Location & Seq

Genomic Coordinates Forward (+)

25964710 .. 25970203

Gene Structure 5494 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1166000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGATGGATGGGCGGCGACATTCTGTTGATATTCCTATCTCGAAAACTCTCGTGCAACTGAGGAGGGTGAGGTCTCTAAGGGATCCTGATACTAATTCTATGAGCAAGTTCTCTGCTTTGGTTGAGAATGTGAACTGGGAAGCGAATTCGAGTAACGACATTTCTGTGCGGTTCATGAATAGTTTCCAAGAAGCTGGCTCTGGCAAACATCATTCTTTTAGATCAAAGAATCCAGGTCTGTATGGACAAGGAGGAGATTGCATAGATGATTTTGAATTAGATCGTGGTTTAGGAAAATCTAGGTTGATCTTGCATGAGAATTCCGAGTGGATTGGAAGCACAGGGAGTCGTCCAATTAGGAGCAAACAAGCTGAAGAATTTGATTTTTCAGTATCAGATAAGGAAGATGTTTATGGAAATAAATCACTCAGCGATAGGTACTGCGGCAGTCAAATGGATAAAGGATTGGCCTTGACAGGCGTCAATCGTTTGGAGGATGGGGATTATGAAGCATCTGTTAGGTCATCAAATTTAGAAAGATTAGATCAGATTGCATCAAAGAGACAATCACAACGCAAAAATAATGTAAATTCGTCCAGGAAAGTTGGTAATTTTAGTCAAGTTTGCAGTCCGTGCCGCTCAGCAAGTGATGCTCTTTCAAGCCATAGTGCATCTTTATTTGTAAATGAAGAGGCTGATGCTGTGGATCACAATCGTCCTAGTTGCGAGGTCAGCTGCTGCTGGTCAAGAACCCCTCGATTCAGAGAAGCTAATTTTCCTTTCAATGTAGATGAATACCCTCTGCTATACAAGAACGTAGATGAGAGTGCGTTTTATGACCAGAGGAGCTCAAAACACATTCGTAATGAAATGAGTCCACGATCAGAAAACCCTAGGAGTCTGAGTCAGAAATTCAGGCCAAAATCTTTTAATGAATTAGTGGGACAAAATGTAGTAGCAATGTCTCTTATGGGAGCTATCGCTAGAGGGAGAATAACATCATTCTATCTATTCCATGGCCCTCGTGGTACAGGAAAGACATCTGCCTCAAGGATTTTTGCTGCAGCACTAAATTGCCTTTCACATGAGGAGCATAGGCCATGTGGTCTCTGCTGTGAATGTGTTTTGTATTTTTCTGGAAGGAGCAGGGACATCAAGGAAGTTGATTCTGTGAGAATCAATCGGAGAGACAGGGTTAGATCCCTTATTAAGAATGCAGCTATTCCTCCACTTTCGTCACGGTTTAAGGTTTTCATTATTGATGAGTGCCACCTGATGCGCGGGGAAACTTGGGCAACTGTTTTGAATAGCATAGATAACCTTTCTCAACATGTTGTCTTCGTGATGCTCACTCCTGATGTCGATGAGCTTCCCCGAAGTGCAGTGTCACGGTCTCAAAGGTTTCACTTTCCAAGGATTAAGGACGCTGATATTGCAAGCAGATTGGGGAGAATCTGTGTTGAAGAGGGTCTTGAATTTGATCAGGGTGCTGTAGACTTCATTGCTGCCAAGTCAAACGGTTCACTTAGGGATGCAGAGATGATGCTTGATCAGCTAAGTTTGCTTGGTAAAAAAGTCACAATGGGCCTGGCATACGAGCTTATTGGAGTTGTCTCGGATGATGAATTACTCGGTTTGCTAGATCTGGCATTGTCATCTGACACTTCTAATACAGTAATAAGGGCCAGGGAGTTGATGAGATCTCGGATTGATCCCATGCAACTTATATCACAGTTGGCAAATCTTGTTATGGACATTCTTGCTGGTAAATGTCAGGACAGTGCTTCTGAAGTCCGAAAAAAGTTCGGCAGTAGACACACGTCTGAAACGGACTTGCAGAAACTGAGCTATGGATTGAGAATATTATCTGAAACTGAGAAGCAATTAAGGGTTTCCAAAAATCAAGCGACATGGCTTACTGCAGCTCTGCTGCAGCTAAGCTCCGTGGAGTCTTCGTCCTTGGATGGAAATGGTACAAAATTGTGTTTGAGAAGTACACAAGAGAGAGCGACAGCAGAGAGCTTCAAGAATCGTGCCACTTGTTCATGCAATTTAGAGACACCAGACAAGTTGGGCATGCAGAAGGATAGTGATGGAAAGTTGGAAGCCATATGGAAGAGAACTACAGATTTATGCCGATCCAATTCACTCAAGAATTTTCTTAAGAAACAGGGAAAATTGTCTTCCTTGCTTGTTGGTCAAGGTTTAGCAATAGCTGAATTGGAATTCTGCCATCCCGATTTTGTATCTAAGGCTGAGAAGTCATGGAAGGTCATTGCAAATTCACTTCAATCTATATGTGGTTGCAATGTGGAGATCAGGATCAATCTTGTACCTTGTGCTTCTGATTCCAAGTGTGCGAAAGTGAAGAAGTCATCTTTTACGTTGTTCAGCTGTTCCCGCAGAATGCAGCAGAAATCACAATCATCTACAGAACATGGAACTGAGTCAGACTATTCTGAGCACACTTCTGAAAAGCCTATGTTAAGTGACAGACCTACTTTACCTTGCTCGTCTGAGTGTTCTTATCAAGTGCCACATAACTGCTGTGACAAAATGGTGGTGGTAAGTACTTTGAGAAATAGCGAAGGAAATATTCTCAGCACAAGGACTGCCTCATCCCGCAGATCATTTGAAGACAACGCATCAGAGGCACCTGGATTAGTGGTTGATTCTTCGAAGGACGATGGAAGCAATCATGAATGCCATGCTTTATCTTTTGTAGAACCTGAACATCAACCAAACTGTTTTCCTAGAACCTTGAGGCTTCAAAAGAAGTTCCGTTCATCAGATGCTTCTCAGACAACCTGTTGCACCAAACGACAAAACACGATTGCATTATCTAGCCCAAGCAAGACATCTTTTGGCACATGTCTCGTGGGAAATGATTCATATGTTTTCTGCAGTGGCGCTTGTAACAACAGTGGCAGCTGTATAGATGAGAATGCATTGAAGGAGAACTCAGGCGTGCTTTGTTGGAGAACCCCAACGCTTCACTTGGGAAAGGCTTGGCAGTTGAGACATAATCGACAAAACTCGAACCTAGGGTGTTGGGTTCTTCCCTGTGCAACTGCTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

1027

Amino Acids

114.09

Weight (kDa)

8.56

Isoelectric Point (pI)

46.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	317 - 473	1.5e-21	DNA polymerase III, delta subunit
AAA	PF00004	336 - 467	3.1e-08	ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid	PF22608	478 - 518	9.7e-07	DNA polymerase III clamp loader subunit, ATPase lid domain
DnaA_N-like_STI	PF23007	694 - 777	3.1e-31	STICHEL, DnaA_N-like alpha-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001101)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24790 AT4G24790 AT4G24790 AT4G24790 AT4G24790
fragaria_vesca	FvH4_5g06841
malus_domestica	MD06G1160000.v1.1 MD14G1166000.v1.1
prunus_persica	Prupe.5G160500_v2.0.a1
pyrus_communis	pycom06g14310 pycom14g13860 pycom14g13870
rosa_chinensis	RchiOBHm_Chr7g0191751 RchiOBHm_Chr7g0232131
rosa_laevigata	RLG00000001389 RLG00000001431 RLG00000004429
rosa_multiflora	Rmu_co8050558.1_g000001 Rmu_co8237971.1_g000001 Rmu_co8286355.1_g000001 Rmu_co8458339.1_g000001 Rmu_sc0000616.1_g000013 Rmu_sc0003901.1_g000007 Rmu_sc0012403.1_g000007
rosa_roxburghii	Rroxscaffold_3G00228760 Rroxscaffold_3G00263560 Rroxscaffold_3G00263570 Rroxscaffold_3G00263580
rosa_rugosa	Rorug06G0511100 Rorug07G0265100 Rorug07G0265200 Rorug07G0269600
rosa_samantha	Rh7AG120700 Rh7AG417900 Rh7AG418000 Rh7AG418100 Rh7AG418200 Rh7AG424100 Rh7AG424200 Rh7BG122500 Rh7BG397900 Rh7BG398000 Rh7BG398100 Rh7CG125300 Rh7CG437800 Rh7CG437900 Rh7CG444400 Rh7CG444500 Rh7DG124000 Rh7DG411900 Rh7DG412000 Rh7DG412100 Rh7DG412200 Rh7DG416000 Rh7DG416100 Rh7DG416200
rosa_wichuraiana	Rw7G010290 Rw7G034600 Rw7G035070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2653
AccBSI	CCGCTC	1 cut(s)	639
AccI	GTMKAC	2 cut(s)	1494, 1813
AccII	CGCG	1 cut(s)	1281
AciI	CCGC	7 cut(s)	13, 169, 444, 637, 1281, 2401, 2623
AclWI	GGATC	7 cut(s)	77, 90, 714, 1194, 1706, 2133, 2330
AcuI	CTGAAG	2 cut(s)	393, 1809
AcyI	GRCGYC	1 cut(s)	480
AfaI	GTAC	6 cut(s)	440, 1030, 1975, 1996, 2334, 2572
AflII	CTTAAG	1 cut(s)	2162
AflIII	ACRYGT	3 cut(s)	1330, 1818, 2870
AjiI	CACGTC	1 cut(s)	1821
AjnI	CCWGG	5 cut(s)	232, 596, 1587, 1685, 2656
Alw21I	GWGCWC	2 cut(s)	851, 2466
AlwI	GGATC	7 cut(s)	77, 90, 714, 1194, 1706, 2133, 2330
AlwNI	CAGNNNCTG	2 cut(s)	738, 1843
AoxI	GGCC	6 cut(s)	468, 917, 1018, 1097, 1585, 1683
Asp700I	GAANNNNTTC	2 cut(s)	182, 2887
AspA2I	CCTAGG	2 cut(s)	893, 3043
AspLEI	GCGC	2 cut(s)	1281, 2912
AspS9I	GGNCC	3 cut(s)	1019, 1585, 1683
AsuHPI	GGTGA	1 cut(s)	79
AsuII	TTCGAA	1 cut(s)	2678
AvrII	CCTAGG	2 cut(s)	893, 3043
BamHI	GGATCC	1 cut(s)	82
BanI	GGYRCC	1 cut(s)	2653
BanII	GRGCYC	1 cut(s)	851
BauI	CACGAG	3 cut(s)	50, 1023, 2876
BbsI	GAAGAC	4 cut(s)	1330, 1944, 2175, 2643
Bbv12I	GWGCWC	2 cut(s)	851, 2466
BccI	CCATC	6 cut(s)	2, 491, 1958, 2087, 2241, 2681
BcgI	CGANNNNNNTGC	2 cut(s)	2857, 2891
BciT130I	CCWGG	5 cut(s)	234, 598, 1589, 1687, 2658
BclI	TGATCA	2 cut(s)	1480, 1549
BfaI	CTAG	8 cut(s)	300, 720, 894, 984, 1640, 2754, 2844, 3044
BfmI	CTRYAG	7 cut(s)	1062, 1491, 1920, 1931, 2124, 2430, 2902
BfoI	RGCGCY	1 cut(s)	2913
BfrI	CTTAAG	1 cut(s)	2162
BglII	AGATCT	2 cut(s)	1642, 1700
BlnI	CCTAGG	2 cut(s)	893, 3043
BlpI	GCTNAGC	2 cut(s)	640, 1937
BmcAI	AGTACT	1 cut(s)	2572
Bme1390I	CCNGG	5 cut(s)	234, 598, 1589, 1687, 2658
BmgBI	CACGTC	1 cut(s)	1821
BmgT120I	GGNCC	3 cut(s)	1019, 1585, 1683
BmiI	GGNNCC	2 cut(s)	84, 2655
BmrFI	CCNGG	5 cut(s)	234, 598, 1589, 1687, 2658
BmrI	ACTGGG	1 cut(s)	145
BmuI	ACTGGG	1 cut(s)	145
BoxI	GACNNNNGTC	2 cut(s)	479, 1819
BpiI	GAAGAC	4 cut(s)	1330, 1944, 2175, 2643
Bpu1102I	GCTNAGC	2 cut(s)	640, 1937
Bpu14I	TTCGAA	1 cut(s)	2678
BpuEI	CTTGAG	3 cut(s)	1033, 2135, 2782
BsaBI	GATNNNNATC	2 cut(s)	711, 2321
BsaHI	GRCGYC	1 cut(s)	480
BsaI	GGTCTC	3 cut(s)	78, 1112, 1398
BsaJI	CCNNGG	7 cut(s)	893, 1015, 1412, 1686, 1944, 1959, 3043
BsaXI	ACNNNNNCTCC	6 cut(s)	889, 919, 1441, 1471, 1940, 1970
Bse1I	ACTGG	1 cut(s)	140
Bse3DI	GCAATG	4 cut(s)	966, 1500, 2274, 2314
Bse8I	GATNNNNATC	2 cut(s)	711, 2321
BseBI	CCWGG	5 cut(s)	234, 598, 1589, 1687, 2658
BseDI	CCNNGG	7 cut(s)	893, 1015, 1412, 1686, 1944, 1959, 3043
BseGI	GGATG	7 cut(s)	13, 502, 1537, 1624, 1969, 2233, 2618
BseJI	GATNNNNATC	2 cut(s)	711, 2321
BseMI	GCAATG	4 cut(s)	966, 1500, 2274, 2314
BseNI	ACTGG	1 cut(s)	140
BseRI	GAGGAG	4 cut(s)	76, 267, 859, 1103
BsgI	GTGCAG	1 cut(s)	1401
Bsh1236I	CGCG	1 cut(s)	1281
BshFI	GGCC	6 cut(s)	470, 919, 1020, 1099, 1587, 1685
BshNI	GGYRCC	1 cut(s)	2653
BsiHKAI	GWGCWC	2 cut(s)	851, 2466
BslFI	GGGAC	2 cut(s)	957, 1163
BsmFI	GGGAC	2 cut(s)	957, 1163
BsmI	GAATGC	4 cut(s)	1219, 2412, 2708, 2950
BsnI	GGCC	6 cut(s)	470, 919, 1020, 1099, 1587, 1685
Bso31I	GGTCTC	3 cut(s)	78, 1112, 1398
Bsp119I	TTCGAA	1 cut(s)	2678
Bsp1286I	GDGCHC	2 cut(s)	851, 2466
Bsp1720I	GCTNAGC	2 cut(s)	640, 1937
Bsp19I	CCATGG	1 cut(s)	1015
BspACI	CCGC	7 cut(s)	13, 169, 444, 637, 1281, 2401, 2623
BspANI	GGCC	6 cut(s)	470, 919, 1020, 1099, 1587, 1685
BspFNI	CGCG	1 cut(s)	1281
BspHI	TCATGA	2 cut(s)	174, 2698
BspLI	GGNNCC	2 cut(s)	84, 2655
BspMAI	CTGCAG	4 cut(s)	1066, 1924, 1935, 2906
BspPI	GGATC	7 cut(s)	77, 90, 714, 1194, 1706, 2133, 2330
BspT104I	TTCGAA	1 cut(s)	2678
BspT107I	GGYRCC	1 cut(s)	2653
BspTI	CTTAAG	1 cut(s)	2162
BspTNI	GGTCTC	3 cut(s)	78, 1112, 1398
BsrBI	CCGCTC	1 cut(s)	639
BsrDI	GCAATG	4 cut(s)	966, 1500, 2274, 2314
BsrI	ACTGG	1 cut(s)	140
BssECI	CCNNGG	7 cut(s)	893, 1015, 1412, 1686, 1944, 1959, 3043
BssNI	GRCGYC	1 cut(s)	480
BssSI	CACGAG	3 cut(s)	50, 1023, 2876
BssT1I	CCWWGG	5 cut(s)	893, 1015, 1412, 1959, 3043
Bst2BI	CACGAG	3 cut(s)	50, 1023, 2876
Bst2UI	CCWGG	5 cut(s)	234, 598, 1589, 1687, 2658
Bst4CI	ACNGT	9 cut(s)	1242, 1300, 1392, 1520, 1675, 1734, 1781, 2747, 2924
Bst6I	CTCTTC	3 cut(s)	684, 1458, 2111
BstACI	GRCGYC	1 cut(s)	480
BstAFI	CTTAAG	1 cut(s)	2162
BstAPI	GCANNNNNTGC	2 cut(s)	633, 650
BstBI	TTCGAA	1 cut(s)	2678
BstC8I	GCNNGC	4 cut(s)	196, 1438, 2078, 2969
BstDSI	CCRYGG	2 cut(s)	1015, 1944
BstF5I	GGATG	7 cut(s)	13, 502, 1537, 1624, 1969, 2233, 2618
BstFNI	CGCG	1 cut(s)	1281
BstH2I	RGCGCY	1 cut(s)	2913
BstHHI	GCGC	2 cut(s)	1281, 2912
BstMWI	GCNNNNNNNGC	8 cut(s)	633, 650, 732, 1433, 1552, 1561, 2400, 2651
BstNI	CCWGG	5 cut(s)	234, 598, 1589, 1687, 2658
BstNSI	RCATGY	3 cut(s)	1334, 2080, 2874
BstPAI	GACNNNNGTC	2 cut(s)	479, 1819
BstSCI	CCNGG	5 cut(s)	232, 596, 1587, 1685, 2656
BstSFI	CTRYAG	7 cut(s)	1062, 1491, 1920, 1931, 2124, 2430, 2902
BstUI	CGCG	1 cut(s)	1281
BstV2I	GAAGAC	4 cut(s)	1330, 1944, 2175, 2643
BstX2I	RGATCY	4 cut(s)	82, 1199, 1642, 1700
BstXI	CCANNNNNNTGG	1 cut(s)	194
BstYI	RGATCY	4 cut(s)	82, 1199, 1642, 1700
BsuRI	GGCC	6 cut(s)	470, 919, 1020, 1099, 1587, 1685
BtgI	CCRYGG	2 cut(s)	1015, 1944
BtrI	CACGTC	1 cut(s)	1821
BtsCI	GGATG	7 cut(s)	13, 502, 1537, 1624, 1969, 2233, 2618
BtsI	GCAGTG	2 cut(s)	1389, 2911
BtsIMutI	CAGTG	4 cut(s)	1389, 1786, 2911, 2929
Cac8I	GCNNGC	4 cut(s)	196, 1438, 2078, 2969
CaiI	CAGNNNCTG	2 cut(s)	738, 1843
CciI	TCATGA	2 cut(s)	174, 2698
CfoI	GCGC	2 cut(s)	1281, 2912
Cfr13I	GGNCC	3 cut(s)	1019, 1585, 1683
CseI	GACGC	2 cut(s)	469, 1433
Csp6I	GTAC	6 cut(s)	439, 1029, 1974, 1995, 2333, 2571
CviQI	GTAC	6 cut(s)	439, 1029, 1974, 1995, 2333, 2571
Eam1104I	CTCTTC	3 cut(s)	684, 1458, 2111
EarI	CTCTTC	3 cut(s)	684, 1458, 2111
Ecl136II	GAGCTC	1 cut(s)	849
Eco130I	CCWWGG	5 cut(s)	893, 1015, 1412, 1959, 3043
Eco24I	GRGCYC	1 cut(s)	851
Eco31I	GGTCTC	3 cut(s)	78, 1112, 1398
Eco53kI	GAGCTC	1 cut(s)	849
Eco57I	CTGAAG	2 cut(s)	393, 1809
EcoICRI	GAGCTC	1 cut(s)	849
EcoRI	GAATTC	3 cut(s)	145, 319, 2225
EcoRII	CCWGG	5 cut(s)	232, 596, 1587, 1685, 2656
EcoT14I	CCWWGG	5 cut(s)	893, 1015, 1412, 1959, 3043
EcoT22I	ATGCAT	1 cut(s)	2950
EcoT38I	GRGCYC	1 cut(s)	851
ErhI	CCWWGG	5 cut(s)	893, 1015, 1412, 1959, 3043
FalI	AAGNNNNNCTT	2 cut(s)	1455, 1487
FaqI	GGGAC	2 cut(s)	957, 1163
FauI	CCCGC	3 cut(s)	1274, 2408, 2630
FauNDI	CATATG	2 cut(s)	2111, 2893
FbaI	TGATCA	2 cut(s)	1480, 1549
FblI	GTMKAC	2 cut(s)	1494, 1813
FokI	GGATG	7 cut(s)	20, 509, 1544, 1631, 1976, 2220, 2605
FriOI	GRGCYC	1 cut(s)	851
FspBI	CTAG	8 cut(s)	300, 720, 894, 984, 1640, 2754, 2844, 3044
GlaI	GCGC	2 cut(s)	1280, 2911
HaeII	RGCGCY	1 cut(s)	2913
HaeIII	GGCC	6 cut(s)	470, 919, 1020, 1099, 1587, 1685
HgaI	GACGC	2 cut(s)	469, 1433
HhaI	GCGC	2 cut(s)	1281, 2912
Hin1I	GRCGYC	1 cut(s)	480
Hin6I	GCGC	2 cut(s)	1279, 2910
HinP1I	GCGC	2 cut(s)	1279, 2910
HphI	GGTGA	1 cut(s)	79
Hpy166II	GTNNAC	6 cut(s)	133, 878, 1495, 1523, 1814, 1997
Hpy8I	GTNNAC	6 cut(s)	133, 878, 1495, 1523, 1814, 1997
HpyAV	CCTTC	6 cut(s)	1134, 2077, 2263, 2582, 2674, 2947
HpyCH4III	ACNGT	9 cut(s)	1242, 1300, 1392, 1520, 1675, 1734, 1781, 2747, 2924
HpyCH4IV	ACGT	3 cut(s)	816, 1820, 2384
HpyF10VI	GCNNNNNNNGC	8 cut(s)	633, 650, 732, 1433, 1552, 1561, 2400, 2651
HpySE526I	ACGT	3 cut(s)	816, 1820, 2384
Hsp92I	GRCGYC	1 cut(s)	480
HspAI	GCGC	2 cut(s)	1279, 2910
Ksp22I	TGATCA	2 cut(s)	1480, 1549
LmnI	GCTCC	6 cut(s)	360, 846, 974, 1090, 1143, 1946
MaeI	CTAG	8 cut(s)	300, 720, 894, 984, 1640, 2754, 2844, 3044
MaeII	ACGT	3 cut(s)	816, 1820, 2384
MaeIII	GTNAC	7 cut(s)	152, 1236, 1386, 1576, 2489, 2549, 2915
MbiI	CCGCTC	1 cut(s)	639
MflI	RGATCY	4 cut(s)	82, 1199, 1642, 1700
MhlI	GDGCHC	2 cut(s)	851, 2466
MlyI	GAGTC	6 cut(s)	355, 883, 907, 913, 1958, 2456
MmeI	TCCRAC	2 cut(s)	2082, 2957
Mph1103I	ATGCAT	1 cut(s)	2950
MroXI	GAANNNNTTC	2 cut(s)	182, 2887
MseI	TTAA	7 cut(s)	930, 1209, 1245, 1419, 1886, 2163, 2486
MslI	CAYNNNNRTG	1 cut(s)	164
MspA1I	CMGCKG	3 cut(s)	735, 2394, 2931
MspCI	CTTAAG	1 cut(s)	2162
MspR9I	CCNGG	5 cut(s)	234, 598, 1589, 1687, 2658
Mva1269I	GAATGC	4 cut(s)	1219, 2412, 2708, 2950
MvaI	CCWGG	5 cut(s)	234, 598, 1589, 1687, 2658
MvnI	CGCG	1 cut(s)	1281
MwoI	GCNNNNNNNGC	8 cut(s)	633, 650, 732, 1433, 1552, 1561, 2400, 2651
NcoI	CCATGG	1 cut(s)	1015
NdeI	CATATG	2 cut(s)	2111, 2893
NlaIV	GGNNCC	2 cut(s)	84, 2655
NmuCI	GTSAC	5 cut(s)	1236, 1386, 1576, 2489, 2549
NsiI	ATGCAT	1 cut(s)	2950
NspI	RCATGY	3 cut(s)	1334, 2080, 2874
NspV	TTCGAA	1 cut(s)	2678
PaeI	GCATGC	1 cut(s)	2080
PagI	TCATGA	2 cut(s)	174, 2698
PciI	ACATGT	2 cut(s)	1330, 2870
PctI	GAATGC	4 cut(s)	1219, 2412, 2708, 2950
PdmI	GAANNNNTTC	2 cut(s)	182, 2887
PfeI	GAWTC	9 cut(s)	229, 759, 1166, 1176, 1452, 2029, 2348, 2672, 2888
PfoI	TCCNGGA	1 cut(s)	596
PleI	GAGTC	6 cut(s)	354, 882, 906, 912, 1957, 2455
PpsI	GAGTC	6 cut(s)	354, 882, 906, 912, 1957, 2455
PscI	ACATGT	2 cut(s)	1330, 2870
PshAI	GACNNNNGTC	2 cut(s)	479, 1819
Psp124BI	GAGCTC	1 cut(s)	851
Psp6I	CCWGG	5 cut(s)	232, 596, 1587, 1685, 2656
PspGI	CCWGG	5 cut(s)	232, 596, 1587, 1685, 2656
PspN4I	GGNNCC	2 cut(s)	84, 2655
PspPI	GGNCC	3 cut(s)	1019, 1585, 1683
PstI	CTGCAG	4 cut(s)	1066, 1924, 1935, 2906
PstNI	CAGNNNCTG	2 cut(s)	738, 1843
PsuI	RGATCY	4 cut(s)	82, 1199, 1642, 1700
PvuII	CAGCTG	3 cut(s)	735, 2394, 2931
RsaI	GTAC	6 cut(s)	440, 1030, 1975, 1996, 2334, 2572
RsaNI	GTAC	6 cut(s)	439, 1029, 1974, 1995, 2333, 2571
RseI	CAYNNNNRTG	1 cut(s)	164
SacI	GAGCTC	1 cut(s)	851
SaqAI	TTAA	7 cut(s)	930, 1209, 1245, 1419, 1886, 2163, 2486
Sau96I	GGNCC	3 cut(s)	1019, 1585, 1683
ScaI	AGTACT	1 cut(s)	2572
SchI	GAGTC	6 cut(s)	355, 883, 907, 913, 1958, 2456
ScrFI	CCNGG	5 cut(s)	234, 598, 1589, 1687, 2658
SduI	GDGCHC	2 cut(s)	851, 2466
SfcI	CTRYAG	7 cut(s)	1062, 1491, 1920, 1931, 2124, 2430, 2902
SfuI	TTCGAA	1 cut(s)	2678
SmiMI	CAYNNNNRTG	1 cut(s)	164
SmlI	CTYRAG	4 cut(s)	1048, 2150, 2162, 2761
SmoI	CTYRAG	4 cut(s)	1048, 2150, 2162, 2761
SphI	GCATGC	1 cut(s)	2080
SsiI	CCGC	7 cut(s)	13, 169, 444, 637, 1281, 2401, 2623
SspI	AATATT	2 cut(s)	1863, 2596
SspMI	CTAG	8 cut(s)	300, 720, 894, 984, 1640, 2754, 2844, 3044
SstI	GAGCTC	1 cut(s)	851
StyD4I	CCNGG	5 cut(s)	232, 596, 1587, 1685, 2656
StyI	CCWWGG	5 cut(s)	893, 1015, 1412, 1959, 3043
TaaI	ACNGT	9 cut(s)	1242, 1300, 1392, 1520, 1675, 1734, 1781, 2747, 2924
TaiI	ACGT	3 cut(s)	819, 1823, 2387
TaqI	TCGA	7 cut(s)	41, 149, 757, 1362, 2678, 3028, 3038
TatI	WGTACW	2 cut(s)	1994, 2570
TauI	GCSGC	3 cut(s)	16, 447, 639
TfiI	GAWTC	9 cut(s)	229, 759, 1166, 1176, 1452, 2029, 2348, 2672, 2888
Tru1I	TTAA	7 cut(s)	930, 1209, 1245, 1419, 1886, 2163, 2486
Tru9I	TTAA	7 cut(s)	930, 1209, 1245, 1419, 1886, 2163, 2486
TscAI	CASTG	4 cut(s)	1389, 1786, 2911, 2929
TseFI	GTSAC	5 cut(s)	1236, 1386, 1576, 2489, 2549
Tsp45I	GTSAC	5 cut(s)	1236, 1386, 1576, 2489, 2549
TspGWI	ACGGA	4 cut(s)	621, 1844, 1933, 2771
TspRI	CASTG	4 cut(s)	1389, 1786, 2911, 2929
Vha464I	CTTAAG	1 cut(s)	2162
XceI	RCATGY	3 cut(s)	1334, 2080, 2874
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1022
XmaJI	CCTAGG	2 cut(s)	893, 3043
XmiI	GTMKAC	2 cut(s)	1494, 1813
XmnI	GAANNNNTTC	2 cut(s)	182, 2887
XspI	CTAG	8 cut(s)	300, 720, 894, 984, 1640, 2754, 2844, 3044
ZrmI	AGTACT	1 cut(s)	2572
Zsp2I	ATGCAT	1 cut(s)	2950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.