pycom05g17180
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000843
Physical Location & Seq
Forward (+)
20407666 .. 20408704
1039 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17180.1

Sequence Viewer

Length: 723 bp
ATGTTTGGGACAATGTTCTGGAACCTTGGCTCCAAAACAAAAAAGCAAAAGGATCTCTTCAATGCAATTGGTTCGATGTACGCTGCTGTTCTCTTTCTCGGTATCAAAAATTCTACAACTGTGCAGCCTGTTGTGGATGTCGAAAGAACAGTCTTCTACAGAGAACGAGCTGCTGGAATGTATTCTGCCTTGGCATACGCCTTTGCGCAGGTTACAATTGAGATCCCGTATGTTTTTGTACAAGCTCTTTTCTACAGTGTCATAGTTTACGCAACGATTGGGTTTGAATGGACCGTGGCTAAATTCTTGTGGTACCTATTCTTCATGTACTTCACATTTTTGTACTTCACCTACTATGGCATGATGGGTGTGGCCTTGACGCCGAACCAGCACATTGCCGCTATAAGTTCCAGCGCATTTTACGCAATATGGAATCTCTTCTCAGGGTTCATAATCCCACGAACTAGGATTCCTGTATGGTGGAGGTGGTACTACTGGGCGTGCCCGATGGCTTGGACCTTGTACGGACTGGCTACATCGCAGTTTGGAGATATACAGGACAAGCTCGAGACGGGTGAAACGGTGGAAGAATTCATGAGGCAATACTTCGGCTTCAAGCAAGAATTTATCGGAGTGGTTGCAGCTGTGGTTGTTGGATTCACTCTACTCTTTGCACTCATCTTTGCCTTGTCCATCAAGAAGTTGAGCTTCCAGAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

241

Amino Acids

27.88

Weight (kDa)

9.28

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC2_membrane PF01061 1 - 182 2e-50 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 199
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AciI CCGC 1 cut(s) 399
AclWI GGATC 2 cut(s) 60, 217
AcsI RAATTY 4 cut(s) 109, 302, 590, 623
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 7 cut(s) 80, 240, 314, 329, 344, 491, 524
AgsI TTSAA 3 cut(s) 61, 287, 616
AloI GAACNNNNNNTCC 4 cut(s) 14, 46, 454, 486
AluBI AGCT 5 cut(s) 170, 245, 565, 644, 708
AluI AGCT 5 cut(s) 170, 245, 565, 644, 708
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 2 cut(s) 60, 217
Ama87I CYCGRG 1 cut(s) 566
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 4 cut(s) 83, 124, 170, 641
ApoI RAATTY 4 cut(s) 109, 302, 590, 623
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
Asp700I GAANNNNTTC 2 cut(s) 181, 437
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 208, 416
AspS9I GGNCC 2 cut(s) 291, 516
AsuHPI GGTGA 2 cut(s) 340, 587
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 2 cut(s) 291, 516
BaeGI GKGCMC 1 cut(s) 506
BanI GGYRCC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 305, 337
BbsI GAAGAC 1 cut(s) 145
BbvI GCAGC 4 cut(s) 70, 136, 157, 653
BccI CCATC 3 cut(s) 358, 502, 701
BcgI CGANNNNNNTGC 2 cut(s) 54, 88
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 2 cut(s) 157, 253
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 5 cut(s) 84, 125, 171, 399, 642
BlsI GCNGC 5 cut(s) 85, 126, 172, 400, 643
Bme18I GGWCC 2 cut(s) 291, 516
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 2 cut(s) 291, 516
BmiI GGNNCC 3 cut(s) 23, 31, 314
BmrI ACTGGG 1 cut(s) 505
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 25, 189, 294
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 2 cut(s) 500, 534
Bse3DI GCAATG 1 cut(s) 393
BseDI CCNNGG 3 cut(s) 25, 189, 294
BseGI GGATG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 2 cut(s) 500, 534
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 4 cut(s) 70, 136, 157, 653
BsgI GTGCAG 1 cut(s) 143
BshFI GGCC 1 cut(s) 374
BshNI GGYRCC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 566
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 563
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 506
Bsp1407I TGTACA 1 cut(s) 238
Bsp143I GATC 2 cut(s) 52, 222
BspACI CCGC 1 cut(s) 399
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 455
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 3 cut(s) 23, 31, 314
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 2 cut(s) 60, 217
BspT107I GGYRCC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 393
BsrGI TGTACA 1 cut(s) 238
BsrI ACTGG 2 cut(s) 500, 534
BssECI CCNNGG 3 cut(s) 25, 189, 294
BssMI GATC 2 cut(s) 52, 222
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 2 cut(s) 25, 189
Bst4CI ACNGT 5 cut(s) 121, 151, 257, 295, 583
Bst6I CTCTTC 2 cut(s) 62, 443
BstACI GRCGYC 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 1 cut(s) 442
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 142
BstHHI GCGC 2 cut(s) 208, 416
BstKTI GATC 2 cut(s) 55, 225
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 2 cut(s) 52, 222
BstMWI GCNNNNNNNGC 2 cut(s) 388, 422
BstSFI CTRYAG 2 cut(s) 157, 253
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 4 cut(s) 70, 136, 157, 653
BstV2I GAAGAC 1 cut(s) 145
BstX2I RGATCY 2 cut(s) 52, 222
BstYI RGATCY 2 cut(s) 52, 222
BsuRI GGCC 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 294
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 262
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 502
CciI TCATGA 1 cut(s) 594
CfoI GCGC 2 cut(s) 208, 416
Cfr13I GGNCC 2 cut(s) 291, 516
CseI GACGC 1 cut(s) 388
Csp6I GTAC 7 cut(s) 79, 239, 313, 328, 343, 490, 523
CviAII CATG 3 cut(s) 325, 361, 595
CviQI GTAC 7 cut(s) 79, 239, 313, 328, 343, 490, 523
DdeI CTNAG 1 cut(s) 442
DpnI GATC 2 cut(s) 54, 224
DpnII GATC 2 cut(s) 52, 222
Eam1104I CTCTTC 2 cut(s) 62, 443
EarI CTCTTC 2 cut(s) 62, 443
Eco130I CCWWGG 2 cut(s) 25, 189
Eco47I GGWCC 2 cut(s) 291, 516
Eco88I CYCGRG 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 590
EcoT14I CCWWGG 2 cut(s) 25, 189
ErhI CCWWGG 2 cut(s) 25, 189
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 3 cut(s) 328, 364, 598
FalI AAGNNNNNCTT 3 cut(s) 41, 73, 692
FaqI GGGAC 1 cut(s) 22
FatI CATG 3 cut(s) 324, 360, 594
Fnu4HI GCNGC 5 cut(s) 84, 125, 171, 399, 642
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 5 cut(s) 84, 125, 171, 399, 642
FspBI CTAG 1 cut(s) 465
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 2 cut(s) 207, 415
GluI GCNGC 5 cut(s) 84, 125, 171, 399, 642
HaeIII GGCC 1 cut(s) 374
HgaI GACGC 1 cut(s) 388
HhaI GCGC 2 cut(s) 208, 416
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 3 cut(s) 328, 364, 598
Hin6I GCGC 2 cut(s) 206, 414
HinP1I GCGC 2 cut(s) 206, 414
HinfI GANTC 3 cut(s) 433, 469, 657
HphI GGTGA 2 cut(s) 340, 587
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 632
Hpy188III TCNNGA 5 cut(s) 19, 568, 595, 697, 712
Hpy8I GTNNAC 1 cut(s) 268
HpyCH4III ACNGT 5 cut(s) 121, 151, 257, 295, 583
HpyCH4V TGCA 4 cut(s) 65, 124, 641, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 388, 422
HpyF3I CTNAG 1 cut(s) 442
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 3 cut(s) 328, 364, 598
HspAI GCGC 2 cut(s) 206, 414
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 52, 222
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 4 cut(s) 70, 136, 157, 653
MaeI CTAG 1 cut(s) 465
MaeIII GTNAC 1 cut(s) 211
MalI GATC 2 cut(s) 54, 224
MboI GATC 2 cut(s) 52, 222
MboII GAAGA 5 cut(s) 49, 145, 313, 430, 599
MfeI CAATTG 2 cut(s) 66, 216
MflI RGATCY 2 cut(s) 52, 222
MhlI GDGCHC 1 cut(s) 506
MluCI AATT 6 cut(s) 66, 109, 216, 302, 590, 623
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 3 cut(s) 477, 591, 708
MroXI GAANNNNTTC 2 cut(s) 181, 437
MspA1I CMGCKG 1 cut(s) 644
MunI CAATTG 2 cut(s) 66, 216
MwoI GCNNNNNNNGC 2 cut(s) 388, 422
NdeII GATC 2 cut(s) 52, 222
NlaIII CATG 3 cut(s) 328, 364, 598
NlaIV GGNNCC 3 cut(s) 23, 31, 314
NsbI TGCGCA 1 cut(s) 207
PaeR7I CTCGAG 1 cut(s) 566
PagI TCATGA 1 cut(s) 594
PdmI GAANNNNTTC 2 cut(s) 181, 437
PfeI GAWTC 3 cut(s) 433, 469, 657
PkrI GCNGC 5 cut(s) 85, 126, 172, 400, 643
PspN4I GGNNCC 3 cut(s) 23, 31, 314
PspPI GGNCC 2 cut(s) 291, 516
PsuI RGATCY 2 cut(s) 52, 222
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 7 cut(s) 80, 240, 314, 329, 344, 491, 524
RsaNI GTAC 7 cut(s) 79, 239, 313, 328, 343, 490, 523
SatI GCNGC 5 cut(s) 84, 125, 171, 399, 642
Sau3AI GATC 2 cut(s) 52, 222
Sau96I GGNCC 2 cut(s) 291, 516
SduI GDGCHC 1 cut(s) 506
SfcI CTRYAG 2 cut(s) 157, 253
Sfr274I CTCGAG 1 cut(s) 566
SinI GGWCC 2 cut(s) 291, 516
SlaI CTCGAG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 6 cut(s) 66, 109, 216, 302, 590, 623
SsiI CCGC 1 cut(s) 399
SspMI CTAG 1 cut(s) 465
StyI CCWWGG 2 cut(s) 25, 189
TaaI ACNGT 5 cut(s) 121, 151, 257, 295, 583
TaqI TCGA 3 cut(s) 74, 141, 567
TasI AATT 6 cut(s) 66, 109, 216, 302, 590, 623
TatI WGTACW 3 cut(s) 238, 327, 342
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 3 cut(s) 433, 469, 657
TscAI CASTG 1 cut(s) 262
TseI GCWGC 4 cut(s) 83, 124, 170, 641
TspDTI ATGAA 3 cut(s) 313, 439, 583
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 291, 516
XapI RAATTY 4 cut(s) 109, 302, 590, 623
XhoI CTCGAG 1 cut(s) 566
XmnI GAANNNNTTC 2 cut(s) 181, 437
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.