Rroxscaffold_7G00182370
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000843
Physical Location & Seq
Reverse (-)
21328469 .. 21335108
6640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182370.1

Sequence Viewer

Length: 4254 bp
ATGGAGATCATCAGCTGTGACCTTGAAAAAGCCGGTCACAGCTTCAACAGAAGGGGTTCGTTTTGGACTGACGATGGCAGGGCGGTTTTCTCAGCATCTTCAGTTGGAGAGGACGACGAGGAAGCTCTCAAATGGGCAGCTTTGCAGAGACTTCCTACATTCACTCGATTGAAAAAAGGTCTGCTCACTACTCCTCAAGGCCATGCCAACGAAGTCGATGTGCATCACCTTGAGCTGCAAGAAAAGAAAGACTTGATCGAGAGGCTAGTGGGAATTGCTGAGGAGGATCATGAGAAATTTTTGTTGAGGCTTAAGAGCCGAATCAATAGAGTTGGAATTAGTCTTCCTACAATTGAAGTTAGGTTCGAGCATTTGAAAATTGCAGCTGAAACTCATGTTGGAGGCAGGGCTCTGCCTTCTGTCTTCAATTATTGTGTTAATGTAGTGGAGGGTTTCTTGAATTGCCTGCACATTCTTCCTGAAAAGAAGGAACATTTGTCTATACTGAAAGATGTCAGTGGGATCATCAAGCCTTGCAGGATGACATTGCTTTTGGGTCCTCCAAGTTCGGGGAAGACCACGCTCTTGCTGGCTTTGGCCGGAGAGCTTGATAAGGATCTCAAGGTTGTATTAAATGAAATGGGCCTTTTAACTAAGGTTTCAGGGAGTGTGACTTACAATGGTCATGGCATGCACGAGTTTGTGCCTCAGAGAAGTGCTGCATATATCAGTCAACATGATGTTCATATTGGAGAAATGACAGTTGCAGAAACCTTGGCTTTTTCTGCAAGATGCCAAGGGGTCGGACCTCGTTATGAGATGCTAGCAGAGCTAAGTAGAAGAGAAAAAGATGCAAGTATTAAGCCAGATCCAGACTTGGATATCTACATGAAGGGAATGGCATCAGAAAGTGAGAGAGCAGCATTTGTGACAAATTATATTCTGAAGGTTTTGGGATTGGAGGGCTGTGCAAATACCTTGGTAGGGGATCAATTGATAAGGGGTATCTCTGGAGGACAAAAGAAGCGAGTCACAACTGGTGAGATGTTAGTTGGTCCTGCTAAGGCACTATTCATGGACGAAATATCGACTGGTTTGGATAGTTCAACAACTTATCAAATAGTGAATTCCATCAAGCAATATGTTCACATTCTCCAAGGGACTGCATTCATCTCACTACTGCAGCCAGCACCCGAGACTTATGATCTATTTGATGACATTATTCTCCTTTCTGATGGTCAAATTGTGTATCAAGGTCCCCGTGAACATGTTCTCGATTTTTTTGAGTCTATGGGCTTCAGATGTCCACAGAGAAAAGGTGTGGCCGATTTCCTGCAAGAAGTGACATCAAGGAAAGATCAGGAGCAGTATTGGTTTAGCAGAGATGAACCTTACAGGTTCATCACAGTCGAAGAATTTTCCGTGGCATTCCAATCTTTCCACGTAGGGCGCGCAATTACACATGAACTTGCTACTCCATTTGACAAGACCAAGAGCCACTCAGCTGCATTAACGACAAAAAAATATGGTGTTAAAAGAGCAGAACTGCTGAAAGCTTGTTTCTCAAGGGAGTTCTTGCTCATGAAGAGGAATTCATTTGTTTACCTTTTCAAGCTTATCCAACTCTCAATATTGTCTCTGATTACAATGACACTGTTCCTACGAACTGAGATGCACCGCGATTCAGTAATTAATGGAGGAATTTATGCTGGCGCATTATTCTTCTCCTTGGTTGCTGTTATGTTCAATGGAATGGCAGAGCTTTCGATGACTATAGCCAAGCTTCCAGTGTTTTATAAGCACAGAAAGCTGCTCTTCTTTCCACCATGGGCATATGCACTTCCGGCATGGCTCCTCAAGATCCCTCTCACCTGTGTTGAAGTTGGTGTTTGGGTATTTATCACCTATTATGTCATTGGCTTTGATCCAAATGTTGGAAGACTGTTTAAGCAATACCTTCTCCTCCTACTCATGAATCAAATGGCTTCTGGATTATTCCGATTCATTGCTGGAGTAGGTAGAAGCTTGACTGTTGCTAATACATTTGGCTCATTTGCACTAGTCATGCTTTTCGCTTTGGGGGCTTTGTCCTGTCAAGAGAATGCAATTGTAGTTAATGAATTCCTTGGCAAGAGTTGGTCACATGTTCTTCCAAACTCAAACGAATCATTAGGAATTGAAATTCTGAAATCTCGTGGATTTCGTACACATGCATATTGGTATTGGATTGGCGCAGGAGCATTGGCTGGATACATGCTCTTATTCAACATTTGTTACACTCTGGCTCTCACTTATCTAAACCCTTTGGACAAGCCACAGGGTGTTAAATCGGAAGAATCTCAAGGAAATAAAGATGATGGCAAAAGCTTGACTCCTCAAATCAGCAAAGAGAAAGGAGATGGAAGTACTACGCATAACACGAAAAGAGGAATGGTTCTTCCATTTGAACCTTATTCCATCACCTTTGATGAAATCTCATACTCGGTGGACATGCCACAGGAAATGAAGAACCAAGGTGTTCCTGAGGATAAATTGGTGCTCCTCAGGCGCGTGAGTGGTGCTTTCAGGCCAGGCGTTCTCACAGCTCTTATGGGAGTGAGTGGTGCTGGCAAGACAACTCTAATGGATGTACTAGCTGGTAGAAAAACTGGGGGATATATTGAGGGAAATATCACAATTTCTGGGCACCCCAAGAAGCAAGAATCATTTGCTCGGATTTCTGGATACTGTGAGCAGAATGACATCCACTCTCCACATGTTACTGTTTATGAATCCTTGATGTACTCAGCATGGTTGCGTTTACCTGCAGAAATCGATTCTGAAATGAGGAAGATGTTTGTCGAGGAAGTGATGGCACTTGTGGAGTTGGATCCGTTAAGGCAAGCACTTGTAGGGTTGCCTGGTGCAAATGGTCTCTCAACTGAGCAAAGAAAGAGGCTGACCATTGCAGTTGAACTAGTGGCTAACCCCTCCATAATTTTCATGGATGAACCAACTTCTGGCCTAGATGCAAGAGCTGCTGCAATTGTTATGAGAACAGTTAGGAATACTGTGGACACCGGAAGAACAGTTGTGTGCACCATTCATCAGCCAAGCATTGATATCTTTGAATCTTTTGATGAGTTATTCCTACTGAAGAAAGGAGGACAAGAGCTATACGTTGGGCCATTGGGGCGTCATTCTTGCCATTTAATCAAGTACTTTGAGGGAATTGATAATGTCAGTAAGATTAAGGATGGTTATAATCCGGCAACTTGGATGTTGGAAGTCACCAGTTCTGCGAAAGAAATAGCTTCTGGTGTTGATTTTGCTGAGATTTATAAAAATTCAGAGCTATACAGGAGAAACAAAACACTCATTGAAGAACTGAGCACCCCTCCTCCAGATTCCAAGGACCTGTATTTCCCTACTCAATATTCTCAGCCATTTTTAACACAATGCATGGCTTGCTTATGGAAACAACATTGGTCGTATTGGCGCAATCCAGCATATACTGCCATAAAGTTGATATACACAACCATCATAGCCTTGATGTTGGGGACAATGTTCTGGGACCTTGGCTCCAAAACGAAAAAACAACAGGATCTGTTCAATGCAATTGGTTCTATGTATGCTGCTGTTCTGTTTCTTGGTATTAAGAATTCAACAACAGTTCAGCCAGTAGTGTCTGTCGAACGAACAGTCTTTTATAGAGAGCGAGCTGCTGGAATGTATTCTCCATTGGCTTATGCATTTGCACAGGTTGCAATTGAGCTCCCATATATTTTTGCCCAAACAATTCTCTATGGTGTCATAGTCTATGCAATGATCGGTTTTGAGTGGACTGCAACTAAATTCTTATGGTACCTATTTTTCATGTATTTCACATTTCTGTACTTCACCTTCTATGGTATGATGGGAGTGGCCTTTTCACCGAACCAGCACGTTGCTGCTATAAGCTCTAGTGCATTTTATGCAGTATGGAATGTCTTCTCCGGGTTCTTAATCCCACGAACTAGGATTCCTATATGGTGGAGATGGTACTATTGGGCATGTCCAATGGCTTGGAGCTTGTATGGATTGGCTACATCACAGTTTGGAGATCTACAGAACGAGCTTGAGACAGGCGAAACTGTGGAACAATTTATGAGGACTTACTTTGGCTTCAAACATGAATTTCTAGGAGTGGTTGCAGCAGTAGTTGCTGGATTTGCTGTACTCTTTGCATTTGTCTTTGCCTTTTCAATTAAGATGTTGAACTTCCAGAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1417

Amino Acids

159.43

Weight (kDa)

7.37

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 169 - 361 3.9e-13 ABC transporter
ABC2_membrane PF01061 516 - 694 1.5e-35 ABC-2 type transporter
PDR_assoc PF08370 713 - 774 1.9e-28 Plant PDR ABC transporter associated
ABC_tran PF00005 848 - 1000 3.8e-21 ABC transporter
ABC2_membrane PF01061 1145 - 1359 1.1e-58 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1797, 3243, 3321
Acc36I ACCTGC 1 cut(s) 2812
Acc65I GGTACC 1 cut(s) 3843
AccB1I GGYRCC 2 cut(s) 2685, 3843
AccB7I CCANNNNNTGG 2 cut(s) 1934, 2999
AccII CGCG 3 cut(s) 1452, 1680, 2550
AciI CCGC 2 cut(s) 83, 1678
AcoI YGGCCR 2 cut(s) 597, 1323
AcuI CTGAAG 4 cut(s) 84, 965, 1282, 3155
AcyI GRCGYC 1 cut(s) 3175
AdeI CACNNNGTG 1 cut(s) 2321
AfaI GTAC 9 cut(s) 2206, 2407, 2631, 2783, 3200, 3845, 3875, 4022, 4197
AfiI CCNNNNNNNGG 4 cut(s) 569, 984, 1934, 2999
AflII CTTAAG 1 cut(s) 311
AflIII ACRYGT 3 cut(s) 1267, 2143, 2755
AhlI ACTAGT 2 cut(s) 2059, 2956
AjnI CCWGG 2 cut(s) 2569, 2899
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AloI GAACNNNNNNTCC 2 cut(s) 3985, 4017
Alw21I GWGCWC 4 cut(s) 2541, 3080, 3374, 3756
Alw26I GTCTC 5 cut(s) 142, 1190, 1641, 2918, 4094
Alw44I GTGCAC 1 cut(s) 3076
AlwNI CAGNNNCTG 1 cut(s) 3586
Ama87I CYCGRG 1 cut(s) 1193
AoxI GGCC 8 cut(s) 199, 597, 643, 1323, 2567, 3001, 3164, 3903
ApaLI GTGCAC 1 cut(s) 3076
ArsI GACNNNNNNTTYG 4 cut(s) 535, 567, 3666, 3698
AseI ATTAAT 1 cut(s) 1692
Asp700I GAANNNNTTC 4 cut(s) 55, 1269, 3712, 3968
Asp718I GGTACC 1 cut(s) 3843
AspLEI GCGC 6 cut(s) 1452, 1454, 1715, 2234, 2550, 3480
AspS9I GGNCC 8 cut(s) 557, 643, 806, 1055, 1256, 3164, 3394, 3553
AsuC2I CCSGG 1 cut(s) 3976
AsuHPI GGTGA 8 cut(s) 218, 1052, 1861, 1894, 2452, 3262, 3871, 3903
AsuNHI GCTAGC 1 cut(s) 823
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 6 cut(s) 557, 806, 1055, 1256, 3394, 3553
AxyI CCTNAGG 2 cut(s) 2523, 2543
BaeGI GKGCMC 2 cut(s) 2688, 3080
BamHI GGATCC 1 cut(s) 2869
BanI GGYRCC 2 cut(s) 2685, 3843
BanII GRGCYC 2 cut(s) 412, 3756
BarI GAAGNNNNNNTAC 2 cut(s) 3866, 3898
BauI CACGAG 2 cut(s) 695, 2193
BbsI GAAGAC 5 cut(s) 335, 415, 581, 1945, 3961
Bbv12I GWGCWC 4 cut(s) 2541, 3080, 3374, 3756
BbvCI CCTCAGC 1 cut(s) 279
BcgI CGANNNNNNTGC 2 cut(s) 1746, 1780
BciT130I CCWGG 2 cut(s) 2571, 2901
BciVI GTATCC 2 cut(s) 2243, 2717
BcnI CCSGG 1 cut(s) 3976
BcoDI GTCTC 5 cut(s) 142, 1190, 1641, 2918, 4094
BcuI ACTAGT 2 cut(s) 2059, 2956
BfaI CTAG 9 cut(s) 266, 824, 2060, 2633, 2957, 3005, 3942, 3996, 4160
BfmI CTRYAG 4 cut(s) 1181, 1773, 2805, 4085
BfrI CTTAAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 2812
BfuI GTATCC 2 cut(s) 2243, 2717
BglI GCCNNNNNGGC 1 cut(s) 3172
BglII AGATCT 1 cut(s) 4081
BmcAI AGTACT 2 cut(s) 2407, 3200
Bme1390I CCNGG 3 cut(s) 2571, 2901, 3976
Bme18I GGWCC 6 cut(s) 557, 806, 1055, 1256, 3394, 3553
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 8 cut(s) 557, 643, 806, 1055, 1256, 3164, 3394, 3553
BmiI GGNNCC 8 cut(s) 558, 1258, 1853, 2687, 2871, 3554, 3562, 3845
BmrFI CCNGG 3 cut(s) 2571, 2901, 3976
BmrI ACTGGG 1 cut(s) 2658
BmsI GCATC 8 cut(s) 104, 232, 782, 810, 841, 911, 1662, 2998
BmtI GCTAGC 1 cut(s) 827
BmuI ACTGGG 1 cut(s) 2658
BpiI GAAGAC 5 cut(s) 335, 415, 581, 1945, 3961
BplI GAGNNNNNCTC 2 cut(s) 3361, 3393
BpmI CTGGAG 3 cut(s) 1032, 2031, 3366
Bpu10I CCTNAGC 2 cut(s) 279, 1062
BpuEI CTTGAG 7 cut(s) 180, 251, 605, 1549, 1841, 2325, 4118
BpuMI CCSGG 1 cut(s) 3976
Bsa29I ATCGAT 1 cut(s) 2814
BsaAI YACGTR 1 cut(s) 1444
BsaBI GATNNNNATC 2 cut(s) 222, 615
BsaHI GRCGYC 1 cut(s) 3175
BsaI GGTCTC 1 cut(s) 2918
BsaWI WCCGGW 1 cut(s) 3059
BsaXI ACNNNNNCTCC 4 cut(s) 3364, 3394, 3545, 3575
Bsc4I CCNNNNNNNGG 4 cut(s) 569, 984, 1934, 2999
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 6 cut(s) 1042, 1096, 1787, 2653, 3273, 3659
Bse21I CCTNAGG 2 cut(s) 2523, 2543
Bse3DI GCAATG 4 cut(s) 545, 2004, 2943, 3810
Bse8I GATNNNNATC 2 cut(s) 222, 615
BseBI CCWGG 2 cut(s) 2571, 2901
BseCI ATCGAT 1 cut(s) 2814
BseGI GGATG 6 cut(s) 546, 2632, 2742, 2992, 3241, 3264
BseJI GATNNNNATC 2 cut(s) 222, 615
BseLI CCNNNNNNNGG 4 cut(s) 569, 984, 1934, 2999
BseMI GCAATG 4 cut(s) 545, 2004, 2943, 3810
BseNI ACTGG 6 cut(s) 1042, 1096, 1787, 2653, 3273, 3659
BsePI GCGCGC 1 cut(s) 1450
BseRI GAGGAG 7 cut(s) 183, 296, 1844, 1952, 2364, 2531, 3369
BseSI GKGCMC 2 cut(s) 2688, 3080
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 3 cut(s) 1452, 1680, 2550
BshFI GGCC 8 cut(s) 201, 599, 645, 1325, 2569, 3003, 3166, 3905
BshNI GGYRCC 2 cut(s) 2685, 3843
BshVI ATCGAT 1 cut(s) 2814
BsiHKAI GWGCWC 4 cut(s) 2541, 3080, 3374, 3756
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 6 cut(s) 33, 600, 1844, 3060, 3248, 3975
BslFI GGGAC 4 cut(s) 1174, 1242, 3553, 3566
BslI CCNNNNNNNGG 4 cut(s) 569, 984, 1934, 2999
BsmAI GTCTC 5 cut(s) 142, 1190, 1641, 2918, 4094
BsmFI GGGAC 4 cut(s) 1174, 1242, 3553, 3566
BsmI GAATGC 3 cut(s) 1166, 1427, 2107
BsnI GGCC 8 cut(s) 201, 599, 645, 1325, 2569, 3003, 3166, 3905
Bso31I GGTCTC 1 cut(s) 2918
BsoBI CYCGRG 1 cut(s) 1193
Bsp1286I GDGCHC 6 cut(s) 412, 2541, 2688, 3080, 3374, 3756
Bsp19I CCATGG 1 cut(s) 1826
BspACI CCGC 2 cut(s) 83, 1678
BspANI GGCC 8 cut(s) 201, 599, 645, 1325, 2569, 3003, 3166, 3905
BspDI ATCGAT 1 cut(s) 2814
BspFNI CGCG 3 cut(s) 1452, 1680, 2550
BspHI TCATGA 3 cut(s) 289, 1581, 1971
BspLI GGNNCC 8 cut(s) 558, 1258, 1853, 2687, 2871, 3554, 3562, 3845
BspMAI CTGCAG 2 cut(s) 1185, 2809
BspMI ACCTGC 1 cut(s) 2812
BspOI GCTAGC 1 cut(s) 827
BspQI GCTCTTC 1 cut(s) 1820
BspT107I GGYRCC 2 cut(s) 2685, 3843
BspTI CTTAAG 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 2918
BsrDI GCAATG 4 cut(s) 545, 2004, 2943, 3810
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 6 cut(s) 1042, 1096, 1787, 2653, 3273, 3659
BssAI RCCGGY 1 cut(s) 32
BssHII GCGCGC 1 cut(s) 1450
BssNI GRCGYC 1 cut(s) 3175
BssSI CACGAG 2 cut(s) 695, 2193
Bst2BI CACGAG 2 cut(s) 695, 2193
Bst2UI CCWGG 2 cut(s) 2571, 2901
Bst6I CTCTTC 3 cut(s) 835, 1580, 1820
BstACI GRCGYC 1 cut(s) 3175
BstAFI CTTAAG 1 cut(s) 311
BstAPI GCANNNNNTGC 6 cut(s) 3017, 3447, 3494, 3743, 3953, 4181
BstBAI YACGTR 1 cut(s) 1444
BstDSI CCRYGG 2 cut(s) 1422, 1826
BstF5I GGATG 6 cut(s) 546, 2632, 2742, 2992, 3241, 3264
BstFNI CGCG 3 cut(s) 1452, 1680, 2550
BstHHI GCGC 6 cut(s) 1452, 1454, 1715, 2234, 2550, 3480
BstMAI GTCTC 5 cut(s) 142, 1190, 1641, 2918, 4094
BstNI CCWGG 2 cut(s) 2571, 2901
BstNSI RCATGY 8 cut(s) 694, 1271, 2147, 2213, 2257, 2494, 2759, 4035
BstSCI CCNGG 3 cut(s) 2569, 2899, 3974
BstSFI CTRYAG 4 cut(s) 1181, 1773, 2805, 4085
BstSLI GKGCMC 2 cut(s) 2688, 3080
BstUI CGCG 3 cut(s) 1452, 1680, 2550
BstV2I GAAGAC 5 cut(s) 335, 415, 581, 1945, 3961
BstX2I RGATCY 6 cut(s) 616, 868, 1860, 2869, 3583, 4081
BstXI CCANNNNNNTGG 1 cut(s) 4044
BstYI RGATCY 6 cut(s) 616, 868, 1860, 2869, 3583, 4081
Bsu15I ATCGAT 1 cut(s) 2814
Bsu36I CCTNAGG 2 cut(s) 2523, 2543
BsuI GTATCC 2 cut(s) 2243, 2717
BsuRI GGCC 8 cut(s) 201, 599, 645, 1325, 2569, 3003, 3166, 3905
BsuTUI ATCGAT 1 cut(s) 2814
BtgI CCRYGG 2 cut(s) 1422, 1826
BtsCI GGATG 6 cut(s) 546, 2632, 2742, 2992, 3241, 3264
BtsIMutI CAGTG 3 cut(s) 523, 1652, 1794
BveI ACCTGC 1 cut(s) 2812
CaiI CAGNNNCTG 1 cut(s) 3586
CciI TCATGA 3 cut(s) 289, 1581, 1971
CfoI GCGC 6 cut(s) 1452, 1454, 1715, 2234, 2550, 3480
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 8 cut(s) 557, 643, 806, 1055, 1256, 3164, 3394, 3553
ClaI ATCGAT 1 cut(s) 2814
CseI GACGC 1 cut(s) 3164
Csp6I GTAC 9 cut(s) 2205, 2406, 2630, 2782, 3199, 3844, 3874, 4021, 4196
CviQI GTAC 9 cut(s) 2205, 2406, 2630, 2782, 3199, 3844, 3874, 4021, 4196
DraIII CACNNNGTG 1 cut(s) 2321
EaeI YGGCCR 2 cut(s) 597, 1323
Eam1104I CTCTTC 3 cut(s) 835, 1580, 1820
EarI CTCTTC 3 cut(s) 835, 1580, 1820
Ecl136II GAGCTC 1 cut(s) 3754
Eco24I GRGCYC 2 cut(s) 412, 3756
Eco31I GGTCTC 1 cut(s) 2918
Eco32I GATATC 2 cut(s) 883, 3103
Eco47I GGWCC 6 cut(s) 557, 806, 1055, 1256, 3394, 3553
Eco53kI GAGCTC 1 cut(s) 3754
Eco57I CTGAAG 4 cut(s) 84, 965, 1282, 3155
Eco81I CCTNAGG 2 cut(s) 2523, 2543
Eco88I CYCGRG 1 cut(s) 1193
EcoICRI GAGCTC 1 cut(s) 3754
EcoO109I RGGNCCY 4 cut(s) 557, 1256, 3394, 3553
EcoRI GAATTC 4 cut(s) 1126, 1591, 2120, 3640
EcoRII CCWGG 2 cut(s) 2569, 2899
EcoRV GATATC 2 cut(s) 883, 3103
EcoT22I ATGCAT 3 cut(s) 2215, 3443, 3733
EcoT38I GRGCYC 2 cut(s) 412, 3756
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 1799, 1831
FaqI GGGAC 4 cut(s) 1174, 1242, 3553, 3566
FauNDI CATATG 1 cut(s) 1834
FokI GGATG 6 cut(s) 553, 2639, 2729, 2999, 3248, 3271
FriOI GRGCYC 2 cut(s) 412, 3756
FspBI CTAG 9 cut(s) 266, 824, 2060, 2633, 2957, 3005, 3942, 3996, 4160
GlaI GCGC 6 cut(s) 1451, 1453, 1714, 2233, 2549, 3479
GsuI CTGGAG 3 cut(s) 1032, 2031, 3366
HaeIII GGCC 8 cut(s) 201, 599, 645, 1325, 2569, 3003, 3166, 3905
HapII CCGG 6 cut(s) 33, 600, 1844, 3060, 3248, 3975
HgaI GACGC 1 cut(s) 3164
HhaI GCGC 6 cut(s) 1452, 1454, 1715, 2234, 2550, 3480
Hin1I GRCGYC 1 cut(s) 3175
Hin6I GCGC 6 cut(s) 1450, 1452, 1713, 2232, 2548, 3478
HinP1I GCGC 6 cut(s) 1450, 1452, 1713, 2232, 2548, 3478
HincII GTYRAC 1 cut(s) 734
HindII GTYRAC 1 cut(s) 734
HindIII AAGCTT 5 cut(s) 1554, 1613, 1781, 2023, 2365
HpaII CCGG 6 cut(s) 33, 600, 1844, 3060, 3248, 3975
HphI GGTGA 8 cut(s) 218, 1052, 1861, 1894, 2452, 3262, 3871, 3903
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 7 cut(s) 45, 426, 481, 886, 940, 1967, 3892
HpyCH4IV ACGT 3 cut(s) 1443, 3159, 3924
HpySE526I ACGT 3 cut(s) 1443, 3159, 3924
Hsp92I GRCGYC 1 cut(s) 3175
HspAI GCGC 6 cut(s) 1450, 1452, 1713, 2232, 2548, 3478
KpnI GGTACC 1 cut(s) 3847
LguI GCTCTTC 1 cut(s) 1820
LmnI GCTCC 7 cut(s) 1363, 1857, 2237, 2544, 3566, 3759, 4047
LweI GCATC 8 cut(s) 104, 232, 782, 810, 841, 911, 1662, 2998
MaeI CTAG 9 cut(s) 266, 824, 2060, 2633, 2957, 3005, 3942, 3996, 4160
MaeII ACGT 3 cut(s) 1443, 3159, 3924
MfeI CAATTG 6 cut(s) 351, 992, 2106, 3024, 3597, 3747
MflI RGATCY 6 cut(s) 616, 868, 1860, 2869, 3583, 4081
MhlI GDGCHC 6 cut(s) 412, 2541, 2688, 3080, 3374, 3756
MlyI GAGTC 3 cut(s) 1038, 1295, 2365
MmeI TCCRAC 8 cut(s) 85, 313, 379, 784, 1645, 1915, 2847, 3243
Mph1103I ATGCAT 3 cut(s) 2215, 3443, 3733
MroXI GAANNNNTTC 4 cut(s) 55, 1269, 3712, 3968
MspA1I CMGCKG 3 cut(s) 15, 386, 1505
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 6 cut(s) 33, 600, 1844, 3060, 3248, 3975
MspR9I CCNGG 3 cut(s) 2571, 2901, 3976
MunI CAATTG 6 cut(s) 351, 992, 2106, 3024, 3597, 3747
Mva1269I GAATGC 3 cut(s) 1166, 1427, 2107
MvaI CCWGG 2 cut(s) 2571, 2901
MvnI CGCG 3 cut(s) 1452, 1680, 2550
NciI CCSGG 1 cut(s) 3976
NcoI CCATGG 1 cut(s) 1826
NdeI CATATG 1 cut(s) 1834
NheI GCTAGC 1 cut(s) 823
NlaIV GGNNCC 8 cut(s) 558, 1258, 1853, 2687, 2871, 3554, 3562, 3845
NmuCI GTSAC 8 cut(s) 17, 35, 670, 928, 1030, 1342, 2139, 3268
NsiI ATGCAT 3 cut(s) 2215, 3443, 3733
NspI RCATGY 8 cut(s) 694, 1271, 2147, 2213, 2257, 2494, 2759, 4035
PaeI GCATGC 1 cut(s) 694
PagI TCATGA 3 cut(s) 289, 1581, 1971
PauI GCGCGC 1 cut(s) 1450
PciI ACATGT 3 cut(s) 1267, 2143, 2755
PciSI GCTCTTC 1 cut(s) 1820
PcsI WCGNNNNNNNCGW 2 cut(s) 2200, 2417
PctI GAATGC 3 cut(s) 1166, 1427, 2107
PdmI GAANNNNTTC 4 cut(s) 55, 1269, 3712, 3968
PflMI CCANNNNNTGG 2 cut(s) 1934, 2999
PleI GAGTC 3 cut(s) 1037, 1294, 2365
PpsI GAGTC 3 cut(s) 1037, 1294, 2365
Ppu21I YACGTR 1 cut(s) 1444
PpuMI RGGWCCY 4 cut(s) 557, 1256, 3394, 3553
PscI ACATGT 3 cut(s) 1267, 2143, 2755
PshBI ATTAAT 1 cut(s) 1692
PsiI TTATAA 3 cut(s) 1797, 3243, 3321
Psp124BI GAGCTC 1 cut(s) 3756
Psp5II RGGWCCY 4 cut(s) 557, 1256, 3394, 3553
Psp6I CCWGG 2 cut(s) 2569, 2899
PspGI CCWGG 2 cut(s) 2569, 2899
PspN4I GGNNCC 8 cut(s) 558, 1258, 1853, 2687, 2871, 3554, 3562, 3845
PspPI GGNCC 8 cut(s) 557, 643, 806, 1055, 1256, 3164, 3394, 3553
PspPPI RGGWCCY 4 cut(s) 557, 1256, 3394, 3553
PstI CTGCAG 2 cut(s) 1185, 2809
PstNI CAGNNNCTG 1 cut(s) 3586
PsuI RGATCY 6 cut(s) 616, 868, 1860, 2869, 3583, 4081
PteI GCGCGC 1 cut(s) 1450
PvuII CAGCTG 3 cut(s) 15, 386, 1505
RsaI GTAC 9 cut(s) 2206, 2407, 2631, 2783, 3200, 3845, 3875, 4022, 4197
RsaNI GTAC 9 cut(s) 2205, 2406, 2630, 2782, 3199, 3844, 3874, 4021, 4196
SacI GAGCTC 1 cut(s) 3756
SapI GCTCTTC 1 cut(s) 1820
Sau96I GGNCC 8 cut(s) 557, 643, 806, 1055, 1256, 3164, 3394, 3553
ScaI AGTACT 2 cut(s) 2407, 3200
SchI GAGTC 3 cut(s) 1038, 1295, 2365
ScrFI CCNGG 3 cut(s) 2571, 2901, 3976
SduI GDGCHC 6 cut(s) 412, 2541, 2688, 3080, 3374, 3756
SfaNI GCATC 8 cut(s) 104, 232, 782, 810, 841, 911, 1662, 2998
SfcI CTRYAG 4 cut(s) 1181, 1773, 2805, 4085
SinI GGWCC 6 cut(s) 557, 806, 1055, 1256, 3394, 3553
SmlI CTYRAG 8 cut(s) 195, 230, 311, 620, 1564, 1856, 2340, 4097
SmoI CTYRAG 8 cut(s) 195, 230, 311, 620, 1564, 1856, 2340, 4097
SpeI ACTAGT 2 cut(s) 2059, 2956
SphI GCATGC 1 cut(s) 694
SsiI CCGC 2 cut(s) 83, 1678
SspI AATATT 2 cut(s) 1632, 3416
SspMI CTAG 9 cut(s) 266, 824, 2060, 2633, 2957, 3005, 3942, 3996, 4160
SstI GAGCTC 1 cut(s) 3756
StyD4I CCNGG 3 cut(s) 2569, 2899, 3974
TaiI ACGT 3 cut(s) 1446, 3162, 3927
TatI WGTACW 6 cut(s) 2405, 2629, 2781, 3198, 3873, 4195
TscAI CASTG 3 cut(s) 523, 1659, 1794
TseFI GTSAC 8 cut(s) 17, 35, 670, 928, 1030, 1342, 2139, 3268
Tsp45I GTSAC 8 cut(s) 17, 35, 670, 928, 1030, 1342, 2139, 3268
TspGWI ACGGA 2 cut(s) 1411, 2862
TspRI CASTG 3 cut(s) 523, 1659, 1794
Van91I CCANNNNNTGG 2 cut(s) 1934, 2999
Vha464I CTTAAG 1 cut(s) 311
VneI GTGCAC 1 cut(s) 3076
VpaK11BI GGWCC 6 cut(s) 557, 806, 1055, 1256, 3394, 3553
VspI ATTAAT 1 cut(s) 1692
XceI RCATGY 8 cut(s) 694, 1271, 2147, 2213, 2257, 2494, 2759, 4035
XcmI CCANNNNNNNNNTGG 2 cut(s) 586, 2980
XmnI GAANNNNTTC 4 cut(s) 55, 1269, 3712, 3968
XspI CTAG 9 cut(s) 266, 824, 2060, 2633, 2957, 3005, 3942, 3996, 4160
ZrmI AGTACT 2 cut(s) 2407, 3200
Zsp2I ATGCAT 3 cut(s) 2215, 3443, 3733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.