Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2340, 2418 |
| Acc16I | TGCGCA | 1 cut(s) | 1426 |
| Acc36I | ACCTGC | 1 cut(s) | 1909 |
| Acc65I | GGTACC | 1 cut(s) | 2940 |
| AccB1I | GGYRCC | 2 cut(s) | 1782, 2940 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2096 |
| AccII | CGCG | 4 cut(s) | 85, 87, 89, 1647 |
| AciI | CCGC | 1 cut(s) | 89 |
| AclWI | GGATC | 8 cut(s) | 300, 536, 630, 836, 969, 1961, 1974, 2688 |
| AcoI | YGGCCR | 2 cut(s) | 603, 1296 |
| AcsI | RAATTY | 8 cut(s) | 302, 1099, 1388, 1564, 2422, 2737, 2930, 3251 |
| AcuI | CTGAAG | 3 cut(s) | 938, 1255, 2252 |
| AcyI | GRCGYC | 1 cut(s) | 2272 |
| AfaI | GTAC | 7 cut(s) | 1728, 1880, 2297, 2942, 2972, 3119, 3294 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 575, 957, 1923, 2096 |
| AflIII | ACRYGT | 2 cut(s) | 1240, 1852 |
| AhlI | ACTAGT | 1 cut(s) | 2053 |
| AjnI | CCWGG | 2 cut(s) | 1666, 1996 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 387, 419 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 333, 365, 3082, 3114 |
| Alw21I | GWGCWC | 5 cut(s) | 243, 1638, 2177, 2471, 2853 |
| Alw26I | GTCTC | 3 cut(s) | 1163, 2015, 3191 |
| Alw44I | GTGCAC | 1 cut(s) | 2173 |
| AlwI | GGATC | 8 cut(s) | 300, 536, 630, 836, 969, 1961, 1974, 2688 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2683 |
| Ama87I | CYCGRG | 1 cut(s) | 1166 |
| AoxI | GGCC | 7 cut(s) | 205, 603, 1296, 1664, 2098, 2261, 3000 |
| ApaLI | GTGCAC | 1 cut(s) | 2173 |
| ApoI | RAATTY | 8 cut(s) | 302, 1099, 1388, 1564, 2422, 2737, 2930, 3251 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 541, 573, 2763, 2795 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 55, 1242, 2809, 3065 |
| Asp718I | GGTACC | 1 cut(s) | 2940 |
| AspLEI | GCGC | 6 cut(s) | 85, 87, 89, 1427, 1647, 2577 |
| AspS9I | GGNCC | 7 cut(s) | 563, 779, 1028, 1229, 2261, 2491, 2650 |
| AsuC2I | CCSGG | 1 cut(s) | 3073 |
| AsuHPI | GGTGA | 4 cut(s) | 224, 1025, 2359, 2968 |
| AsuNHI | GCTAGC | 1 cut(s) | 796 |
| AvaI | CYCGRG | 1 cut(s) | 1166 |
| AvaII | GGWCC | 6 cut(s) | 563, 779, 1028, 1229, 2491, 2650 |
| AxyI | CCTNAGG | 2 cut(s) | 1620, 1640 |
| BaeGI | GKGCMC | 2 cut(s) | 1785, 2177 |
| BamHI | GGATCC | 1 cut(s) | 1966 |
| BanI | GGYRCC | 2 cut(s) | 1782, 2940 |
| BanII | GRGCYC | 3 cut(s) | 243, 418, 2853 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2963, 2995 |
| BauI | CACGAG | 1 cut(s) | 668 |
| BbsI | GAAGAC | 3 cut(s) | 421, 587, 3058 |
| Bbv12I | GWGCWC | 5 cut(s) | 243, 1638, 2177, 2471, 2853 |
| BbvCI | CCTCAGC | 1 cut(s) | 285 |
| BccI | CCATC | 8 cut(s) | 68, 1112, 1202, 1942, 2327, 2624, 2986, 3108 |
| BciT130I | CCWGG | 2 cut(s) | 1668, 1998 |
| BciVI | GTATCC | 1 cut(s) | 1814 |
| BcnI | CCSGG | 1 cut(s) | 3073 |
| BcoDI | GTCTC | 3 cut(s) | 1163, 2015, 3191 |
| BcuI | ACTAGT | 1 cut(s) | 2053 |
| BfaI | CTAG | 8 cut(s) | 272, 797, 1730, 2054, 2102, 3039, 3093, 3257 |
| BfmI | CTRYAG | 3 cut(s) | 1154, 1902, 3182 |
| BfuAI | ACCTGC | 1 cut(s) | 1909 |
| BfuI | GTATCC | 1 cut(s) | 1814 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2269 |
| BglII | AGATCT | 2 cut(s) | 6, 3178 |
| BmcAI | AGTACT | 2 cut(s) | 2297, 3294 |
| Bme1390I | CCNGG | 3 cut(s) | 1668, 1998, 3073 |
| Bme18I | GGWCC | 6 cut(s) | 563, 779, 1028, 1229, 2491, 2650 |
| BmeT110I | CYCGRG | 1 cut(s) | 1166 |
| BmgT120I | GGNCC | 7 cut(s) | 563, 779, 1028, 1229, 2261, 2491, 2650 |
| BmiI | GGNNCC | 7 cut(s) | 564, 1231, 1784, 1968, 2651, 2659, 2942 |
| BmrFI | CCNGG | 3 cut(s) | 1668, 1998, 3073 |
| BmrI | ACTGGG | 1 cut(s) | 1755 |
| BmsI | GCATC | 7 cut(s) | 110, 238, 755, 783, 814, 884, 2095 |
| BmtI | GCTAGC | 1 cut(s) | 800 |
| BmuI | ACTGGG | 1 cut(s) | 1755 |
| BpiI | GAAGAC | 3 cut(s) | 421, 587, 3058 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2458, 2490 |
| BpmI | CTGGAG | 2 cut(s) | 1005, 2463 |
| Bpu10I | CCTNAGC | 2 cut(s) | 285, 1035 |
| BpuEI | CTTGAG | 5 cut(s) | 257, 302, 611, 1522, 3215 |
| BpuMI | CCSGG | 1 cut(s) | 3073 |
| Bsa29I | ATCGAT | 2 cut(s) | 330, 1911 |
| BsaAI | YACGTR | 1 cut(s) | 1417 |
| BsaBI | GATNNNNATC | 3 cut(s) | 11, 228, 621 |
| BsaHI | GRCGYC | 1 cut(s) | 2272 |
| BsaI | GGTCTC | 1 cut(s) | 2015 |
| BsaJI | CCNNGG | 8 cut(s) | 747, 769, 951, 1129, 1395, 1609, 2487, 2653 |
| BsaWI | WCCGGW | 1 cut(s) | 2156 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 2461, 2491, 2642, 2672 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 575, 957, 1923, 2096 |
| Bse118I | RCCGGY | 1 cut(s) | 32 |
| Bse1I | ACTGG | 5 cut(s) | 1015, 1069, 1750, 2370, 2756 |
| Bse21I | CCTNAGG | 2 cut(s) | 1620, 1640 |
| Bse3DI | GCAATG | 3 cut(s) | 551, 2040, 2907 |
| Bse8I | GATNNNNATC | 3 cut(s) | 11, 228, 621 |
| BseBI | CCWGG | 2 cut(s) | 1668, 1998 |
| BseCI | ATCGAT | 2 cut(s) | 330, 1911 |
| BseDI | CCNNGG | 8 cut(s) | 747, 769, 951, 1129, 1395, 1609, 2487, 2653 |
| BseGI | GGATG | 6 cut(s) | 552, 1729, 1839, 2089, 2338, 2361 |
| BseJI | GATNNNNATC | 3 cut(s) | 11, 228, 621 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 575, 957, 1923, 2096 |
| BseMI | GCAATG | 3 cut(s) | 551, 2040, 2907 |
| BseNI | ACTGG | 5 cut(s) | 1015, 1069, 1750, 2370, 2756 |
| BsePI | GCGCGC | 2 cut(s) | 83, 85 |
| BseRI | GAGGAG | 3 cut(s) | 302, 1628, 2466 |
| BseSI | GKGCMC | 2 cut(s) | 1785, 2177 |
| BsgI | GTGCAG | 1 cut(s) | 458 |
| Bsh1236I | CGCG | 4 cut(s) | 85, 87, 89, 1647 |
| BshFI | GGCC | 7 cut(s) | 207, 605, 1298, 1666, 2100, 2263, 3002 |
| BshNI | GGYRCC | 2 cut(s) | 1782, 2940 |
| BshVI | ATCGAT | 2 cut(s) | 330, 1911 |
| BsiHKAI | GWGCWC | 5 cut(s) | 243, 1638, 2177, 2471, 2853 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1166 |
| BsiSI | CCGG | 5 cut(s) | 33, 606, 2157, 2345, 3072 |
| BslFI | GGGAC | 4 cut(s) | 1147, 1215, 2650, 2663 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 575, 957, 1923, 2096 |
| BsmAI | GTCTC | 3 cut(s) | 1163, 2015, 3191 |
| BsmBI | CGTCTC | 1 cut(s) | 3191 |
| BsmFI | GGGAC | 4 cut(s) | 1147, 1215, 2650, 2663 |
| BsmI | GAATGC | 2 cut(s) | 1139, 1400 |
| BsnI | GGCC | 7 cut(s) | 207, 605, 1298, 1666, 2100, 2263, 3002 |
| Bso31I | GGTCTC | 1 cut(s) | 2015 |
| BsoBI | CYCGRG | 1 cut(s) | 1166 |
| Bsp1286I | GDGCHC | 7 cut(s) | 243, 418, 1638, 1785, 2177, 2471, 2853 |
| BspACI | CCGC | 1 cut(s) | 89 |
| BspANI | GGCC | 7 cut(s) | 207, 605, 1298, 1666, 2100, 2263, 3002 |
| BspDI | ATCGAT | 2 cut(s) | 330, 1911 |
| BspFNI | CGCG | 4 cut(s) | 85, 87, 89, 1647 |
| BspHI | TCATGA | 2 cut(s) | 295, 1554 |
| BspLI | GGNNCC | 7 cut(s) | 564, 1231, 1784, 1968, 2651, 2659, 2942 |
| BspMAI | CTGCAG | 2 cut(s) | 1158, 1906 |
| BspMI | ACCTGC | 1 cut(s) | 1909 |
| BspOI | GCTAGC | 1 cut(s) | 800 |
| BspPI | GGATC | 8 cut(s) | 300, 536, 630, 836, 969, 1961, 1974, 2688 |
| BspQI | GCTCTTC | 1 cut(s) | 423 |
| BspT107I | GGYRCC | 2 cut(s) | 1782, 2940 |
| BspTNI | GGTCTC | 1 cut(s) | 2015 |
| BsrDI | GCAATG | 3 cut(s) | 551, 2040, 2907 |
| BsrFI | RCCGGY | 1 cut(s) | 32 |
| BsrI | ACTGG | 5 cut(s) | 1015, 1069, 1750, 2370, 2756 |
| BssAI | RCCGGY | 1 cut(s) | 32 |
| BssECI | CCNNGG | 8 cut(s) | 747, 769, 951, 1129, 1395, 1609, 2487, 2653 |
| BssHII | GCGCGC | 2 cut(s) | 83, 85 |
| BssNI | GRCGYC | 1 cut(s) | 2272 |
| BssSI | CACGAG | 1 cut(s) | 668 |
| BssT1I | CCWWGG | 7 cut(s) | 747, 769, 951, 1129, 1609, 2487, 2653 |
| Bst2BI | CACGAG | 1 cut(s) | 668 |
| Bst2UI | CCWGG | 2 cut(s) | 1668, 1998 |
| Bst6I | CTCTTC | 3 cut(s) | 423, 808, 1553 |
| BstACI | GRCGYC | 1 cut(s) | 2272 |
| BstAPI | GCANNNNNTGC | 6 cut(s) | 2114, 2544, 2591, 2840, 3050, 3278 |
| BstBAI | YACGTR | 1 cut(s) | 1417 |
| BstDSI | CCRYGG | 1 cut(s) | 1395 |
| BstF5I | GGATG | 6 cut(s) | 552, 1729, 1839, 2089, 2338, 2361 |
| BstFNI | CGCG | 4 cut(s) | 85, 87, 89, 1647 |
| BstHHI | GCGC | 6 cut(s) | 85, 87, 89, 1427, 1647, 2577 |
| BstMAI | GTCTC | 3 cut(s) | 1163, 2015, 3191 |
| BstNI | CCWGG | 2 cut(s) | 1668, 1998 |
| BstNSI | RCATGY | 4 cut(s) | 667, 1244, 1856, 3132 |
| BstSCI | CCNGG | 3 cut(s) | 1666, 1996, 3071 |
| BstSFI | CTRYAG | 3 cut(s) | 1154, 1902, 3182 |
| BstSLI | GKGCMC | 2 cut(s) | 1785, 2177 |
| BstUI | CGCG | 4 cut(s) | 85, 87, 89, 1647 |
| BstV2I | GAAGAC | 3 cut(s) | 421, 587, 3058 |
| BstX2I | RGATCY | 6 cut(s) | 6, 622, 841, 1966, 2680, 3178 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3141 |
| BstYI | RGATCY | 6 cut(s) | 6, 622, 841, 1966, 2680, 3178 |
| Bsu15I | ATCGAT | 2 cut(s) | 330, 1911 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1620, 1640 |
| BsuI | GTATCC | 1 cut(s) | 1814 |
| BsuRI | GGCC | 7 cut(s) | 207, 605, 1298, 1666, 2100, 2263, 3002 |
| BsuTUI | ATCGAT | 2 cut(s) | 330, 1911 |
| BtgI | CCRYGG | 1 cut(s) | 1395 |
| BtsCI | GGATG | 6 cut(s) | 552, 1729, 1839, 2089, 2338, 2361 |
| BtsIMutI | CAGTG | 1 cut(s) | 529 |
| BveI | ACCTGC | 1 cut(s) | 1909 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2683 |
| CciI | TCATGA | 2 cut(s) | 295, 1554 |
| CfoI | GCGC | 6 cut(s) | 85, 87, 89, 1427, 1647, 2577 |
| Cfr10I | RCCGGY | 1 cut(s) | 32 |
| Cfr13I | GGNCC | 7 cut(s) | 563, 779, 1028, 1229, 2261, 2491, 2650 |
| ClaI | ATCGAT | 2 cut(s) | 330, 1911 |
| CseI | GACGC | 1 cut(s) | 2261 |
| Csp6I | GTAC | 7 cut(s) | 1727, 1879, 2296, 2941, 2971, 3118, 3293 |
| CviQI | GTAC | 7 cut(s) | 1727, 1879, 2296, 2941, 2971, 3118, 3293 |
| EaeI | YGGCCR | 2 cut(s) | 603, 1296 |
| Eam1104I | CTCTTC | 3 cut(s) | 423, 808, 1553 |
| EarI | CTCTTC | 3 cut(s) | 423, 808, 1553 |
| Ecl136II | GAGCTC | 2 cut(s) | 241, 2851 |
| Eco130I | CCWWGG | 7 cut(s) | 747, 769, 951, 1129, 1609, 2487, 2653 |
| Eco24I | GRGCYC | 3 cut(s) | 243, 418, 2853 |
| Eco31I | GGTCTC | 1 cut(s) | 2015 |
| Eco32I | GATATC | 2 cut(s) | 856, 2200 |
| Eco47I | GGWCC | 6 cut(s) | 563, 779, 1028, 1229, 2491, 2650 |
| Eco53kI | GAGCTC | 2 cut(s) | 241, 2851 |
| Eco57I | CTGAAG | 3 cut(s) | 938, 1255, 2252 |
| Eco81I | CCTNAGG | 2 cut(s) | 1620, 1640 |
| Eco88I | CYCGRG | 1 cut(s) | 1166 |
| EcoICRI | GAGCTC | 2 cut(s) | 241, 2851 |
| EcoO109I | RGGNCCY | 4 cut(s) | 563, 1229, 2491, 2650 |
| EcoRI | GAATTC | 3 cut(s) | 1099, 1564, 2737 |
| EcoRII | CCWGG | 2 cut(s) | 1666, 1996 |
| EcoRV | GATATC | 2 cut(s) | 856, 2200 |
| EcoT14I | CCWWGG | 7 cut(s) | 747, 769, 951, 1129, 1609, 2487, 2653 |
| EcoT22I | ATGCAT | 2 cut(s) | 2540, 2830 |
| EcoT38I | GRGCYC | 3 cut(s) | 243, 418, 2853 |
| ErhI | CCWWGG | 7 cut(s) | 747, 769, 951, 1129, 1609, 2487, 2653 |
| Esp3I | CGTCTC | 1 cut(s) | 3191 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 242, 274, 631, 663 |
| FaqI | GGGAC | 4 cut(s) | 1147, 1215, 2650, 2663 |
| FokI | GGATG | 6 cut(s) | 559, 1736, 1826, 2096, 2345, 2368 |
| FriOI | GRGCYC | 3 cut(s) | 243, 418, 2853 |
| FspBI | CTAG | 8 cut(s) | 272, 797, 1730, 2054, 2102, 3039, 3093, 3257 |
| FspI | TGCGCA | 1 cut(s) | 1426 |
| GlaI | GCGC | 6 cut(s) | 84, 86, 88, 1426, 1646, 2576 |
| GsuI | CTGGAG | 2 cut(s) | 1005, 2463 |
| HaeIII | GGCC | 7 cut(s) | 207, 605, 1298, 1666, 2100, 2263, 3002 |
| HapII | CCGG | 5 cut(s) | 33, 606, 2157, 2345, 3072 |
| HgaI | GACGC | 1 cut(s) | 2261 |
| HhaI | GCGC | 6 cut(s) | 85, 87, 89, 1427, 1647, 2577 |
| Hin1I | GRCGYC | 1 cut(s) | 2272 |
| Hin6I | GCGC | 6 cut(s) | 83, 85, 87, 1425, 1645, 2575 |
| HinP1I | GCGC | 6 cut(s) | 83, 85, 87, 1425, 1645, 2575 |
| HincII | GTYRAC | 1 cut(s) | 707 |
| HindII | GTYRAC | 1 cut(s) | 707 |
| HindIII | AAGCTT | 2 cut(s) | 1527, 1586 |
| HinfI | GANTC | 9 cut(s) | 327, 1002, 1259, 1799, 1868, 1913, 2207, 2483, 3097 |
| HpaII | CCGG | 5 cut(s) | 33, 606, 2157, 2345, 3072 |
| HphI | GGTGA | 4 cut(s) | 224, 1025, 2359, 2968 |
| Hpy166II | GTNNAC | 7 cut(s) | 707, 1238, 1576, 1898, 2152, 2175, 2919 |
| Hpy188I | TCNGA | 9 cut(s) | 684, 779, 880, 918, 1207, 1274, 1812, 1918, 2428 |
| Hpy8I | GTNNAC | 7 cut(s) | 707, 1238, 1576, 1898, 2152, 2175, 2919 |
| HpyAV | CCTTC | 7 cut(s) | 45, 175, 432, 487, 859, 913, 2989 |
| HpyCH4IV | ACGT | 4 cut(s) | 477, 1416, 2256, 3021 |
| HpySE526I | ACGT | 4 cut(s) | 477, 1416, 2256, 3021 |
| Hsp92I | GRCGYC | 1 cut(s) | 2272 |
| HspAI | GCGC | 6 cut(s) | 83, 85, 87, 1425, 1645, 2575 |
| KpnI | GGTACC | 1 cut(s) | 2944 |
| LguI | GCTCTTC | 1 cut(s) | 423 |
| LmnI | GCTCC | 6 cut(s) | 246, 1336, 1641, 2663, 2856, 3144 |
| LweI | GCATC | 7 cut(s) | 110, 238, 755, 783, 814, 884, 2095 |
| MaeI | CTAG | 8 cut(s) | 272, 797, 1730, 2054, 2102, 3039, 3093, 3257 |
| MaeII | ACGT | 4 cut(s) | 477, 1416, 2256, 3021 |
| MaeIII | GTNAC | 8 cut(s) | 17, 643, 901, 1003, 1315, 1855, 2365, 2923 |
| MauBI | CGCGCGCG | 1 cut(s) | 85 |
| MfeI | CAATTG | 5 cut(s) | 357, 965, 2121, 2694, 2844 |
| MflI | RGATCY | 6 cut(s) | 6, 622, 841, 1966, 2680, 3178 |
| MhlI | GDGCHC | 7 cut(s) | 243, 418, 1638, 1785, 2177, 2471, 2853 |
| MlyI | GAGTC | 2 cut(s) | 1011, 1268 |
| MmeI | TCCRAC | 5 cut(s) | 319, 385, 757, 1944, 2340 |
| Mph1103I | ATGCAT | 2 cut(s) | 2540, 2830 |
| MroXI | GAANNNNTTC | 4 cut(s) | 55, 1242, 2809, 3065 |
| MspA1I | CMGCKG | 2 cut(s) | 392, 1478 |
| MspI | CCGG | 5 cut(s) | 33, 606, 2157, 2345, 3072 |
| MspR9I | CCNGG | 3 cut(s) | 1668, 1998, 3073 |
| MunI | CAATTG | 5 cut(s) | 357, 965, 2121, 2694, 2844 |
| Mva1269I | GAATGC | 2 cut(s) | 1139, 1400 |
| MvaI | CCWGG | 2 cut(s) | 1668, 1998 |
| MvnI | CGCG | 4 cut(s) | 85, 87, 89, 1647 |
| NciI | CCSGG | 1 cut(s) | 3073 |
| NheI | GCTAGC | 1 cut(s) | 796 |
| NlaIV | GGNNCC | 7 cut(s) | 564, 1231, 1784, 1968, 2651, 2659, 2942 |
| NmuCI | GTSAC | 7 cut(s) | 17, 643, 901, 1003, 1315, 2365, 2923 |
| NsbI | TGCGCA | 1 cut(s) | 1426 |
| NsiI | ATGCAT | 2 cut(s) | 2540, 2830 |
| NspI | RCATGY | 4 cut(s) | 667, 1244, 1856, 3132 |
| PaeI | GCATGC | 1 cut(s) | 667 |
| PagI | TCATGA | 2 cut(s) | 295, 1554 |
| PauI | GCGCGC | 2 cut(s) | 83, 85 |
| PciI | ACATGT | 2 cut(s) | 1240, 1852 |
| PciSI | GCTCTTC | 1 cut(s) | 423 |
| PctI | GAATGC | 2 cut(s) | 1139, 1400 |
| PdmI | GAANNNNTTC | 4 cut(s) | 55, 1242, 2809, 3065 |
| PfeI | GAWTC | 7 cut(s) | 327, 1799, 1868, 1913, 2207, 2483, 3097 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2096 |
| PleI | GAGTC | 2 cut(s) | 1010, 1267 |
| PpsI | GAGTC | 2 cut(s) | 1010, 1267 |
| Ppu21I | YACGTR | 1 cut(s) | 1417 |
| PpuMI | RGGWCCY | 4 cut(s) | 563, 1229, 2491, 2650 |
| PscI | ACATGT | 2 cut(s) | 1240, 1852 |
| PsiI | TTATAA | 2 cut(s) | 2340, 2418 |
| Psp124BI | GAGCTC | 2 cut(s) | 243, 2853 |
| Psp5II | RGGWCCY | 4 cut(s) | 563, 1229, 2491, 2650 |
| Psp6I | CCWGG | 2 cut(s) | 1666, 1996 |
| PspGI | CCWGG | 2 cut(s) | 1666, 1996 |
| PspN4I | GGNNCC | 7 cut(s) | 564, 1231, 1784, 1968, 2651, 2659, 2942 |
| PspPI | GGNCC | 7 cut(s) | 563, 779, 1028, 1229, 2261, 2491, 2650 |
| PspPPI | RGGWCCY | 4 cut(s) | 563, 1229, 2491, 2650 |
| PstI | CTGCAG | 2 cut(s) | 1158, 1906 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2683 |
| PsuI | RGATCY | 6 cut(s) | 6, 622, 841, 1966, 2680, 3178 |
| PteI | GCGCGC | 2 cut(s) | 83, 85 |
| PvuII | CAGCTG | 2 cut(s) | 392, 1478 |
| RsaI | GTAC | 7 cut(s) | 1728, 1880, 2297, 2942, 2972, 3119, 3294 |
| RsaNI | GTAC | 7 cut(s) | 1727, 1879, 2296, 2941, 2971, 3118, 3293 |
| SacI | GAGCTC | 2 cut(s) | 243, 2853 |
| SapI | GCTCTTC | 1 cut(s) | 423 |
| Sau96I | GGNCC | 7 cut(s) | 563, 779, 1028, 1229, 2261, 2491, 2650 |
| ScaI | AGTACT | 2 cut(s) | 2297, 3294 |
| SchI | GAGTC | 2 cut(s) | 1011, 1268 |
| ScrFI | CCNGG | 3 cut(s) | 1668, 1998, 3073 |
| SduI | GDGCHC | 7 cut(s) | 243, 418, 1638, 1785, 2177, 2471, 2853 |
| SfaNI | GCATC | 7 cut(s) | 110, 238, 755, 783, 814, 884, 2095 |
| SfcI | CTRYAG | 3 cut(s) | 1154, 1902, 3182 |
| SinI | GGWCC | 6 cut(s) | 563, 779, 1028, 1229, 2491, 2650 |
| SmlI | CTYRAG | 5 cut(s) | 236, 317, 626, 1537, 3194 |
| SmoI | CTYRAG | 5 cut(s) | 236, 317, 626, 1537, 3194 |
| SpeI | ACTAGT | 1 cut(s) | 2053 |
| SphI | GCATGC | 1 cut(s) | 667 |
| SsiI | CCGC | 1 cut(s) | 89 |
| SspI | AATATT | 1 cut(s) | 2513 |
| SspMI | CTAG | 8 cut(s) | 272, 797, 1730, 2054, 2102, 3039, 3093, 3257 |
| SstI | GAGCTC | 2 cut(s) | 243, 2853 |
| StyD4I | CCNGG | 3 cut(s) | 1666, 1996, 3071 |
| StyI | CCWWGG | 7 cut(s) | 747, 769, 951, 1129, 1609, 2487, 2653 |
| TaiI | ACGT | 4 cut(s) | 480, 1419, 2259, 3024 |
| TatI | WGTACW | 5 cut(s) | 1726, 1878, 2295, 2970, 3292 |
| TfiI | GAWTC | 7 cut(s) | 327, 1799, 1868, 1913, 2207, 2483, 3097 |
| TscAI | CASTG | 1 cut(s) | 529 |
| TseFI | GTSAC | 7 cut(s) | 17, 643, 901, 1003, 1315, 2365, 2923 |
| Tsp45I | GTSAC | 7 cut(s) | 17, 643, 901, 1003, 1315, 2365, 2923 |
| TspGWI | ACGGA | 2 cut(s) | 1384, 1959 |
| TspRI | CASTG | 1 cut(s) | 529 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2096 |
| VneI | GTGCAC | 1 cut(s) | 2173 |
| VpaK11BI | GGWCC | 6 cut(s) | 563, 779, 1028, 1229, 2491, 2650 |
| XapI | RAATTY | 8 cut(s) | 302, 1099, 1388, 1564, 2422, 2737, 2930, 3251 |
| XceI | RCATGY | 4 cut(s) | 667, 1244, 1856, 3132 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 592, 2077 |
| XmnI | GAANNNNTTC | 4 cut(s) | 55, 1242, 2809, 3065 |
| XspI | CTAG | 8 cut(s) | 272, 797, 1730, 2054, 2102, 3039, 3093, 3257 |
| ZrmI | AGTACT | 2 cut(s) | 2297, 3294 |
| Zsp2I | ATGCAT | 2 cut(s) | 2540, 2830 |