pycom05g17210
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000843
Physical Location & Seq
Forward (+)
20417334 .. 20420353
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17210.11

Sequence Viewer

Length: 1383 bp
ATGACTATTAATCGAAGATTATTTGTGGTTGGTTTAAATTGTGGTGACATCCTAGTAGAGTTAAGCAGACGAGAGAAAGAAGATAATATCAAGCCGGATGCAGACATAGATATCTACGTGAAGGTGAGAAATAAGAAAAGCTACTTAGCAGAACTTAGATATCTACTTGAGAGTTCTACTAGCAGTGGCATCAGAAAGCCAGAGGGCGCAAGTGGTGACAGAATATATACTAAAGATTTTGGGATTAGATGCGAGATGCTTGTTGGACCGGCTAAGGCGTTATTCATGGACGAAATATCTACGGCACCTGAGACTTATGACCTGTTTGATGACATTGTTCCGCTATCGGGTCACATTGTGTACCAAGGTCCTCGTGAAAATGCTCTTGAATTTTTCGAATCATGGGGTTCAGATGTCCCAAGAGGAAAGGTGACATCAAGGAAAGATCAGGACCAGTATTGGGCAAGCAGAGATGAGCCTTACAGGTTCATTACACATGAACTCGCAATTCCATTTGACAAGGCCAAGAGCCACCCAGCTGCATTAACAACGAAAGAGTATGGCGTTAGTAAAACTGAACTGTTGAAAGCTTGCTTTGCAAGGGAGTTCTTACTCATGAAGAGGAACTCATTTTTCTACCCTTTCAAGCTTGCCCAACTCTCAATCATGGCTATGATTACAATGACATTGTTCCTACGAACTGAGATGCATCGCGATTCAGTAGATGACGGGGTAGTTTATACTGGTGTATTGTTCTTCTCCATGGTCGTTGTTTTGTTCAATGGACTGGCAGATCTTTCGATGACTGTTACAAGGCTCCCGATACATGCTCCGTTCGTACCACATTCCATCACCTTTGATGAAATCACATACTCCGTTGACGTGCCACAGAAGGAAATGAAGAATGAAGGTGTTCCCGAGGATAAACTGGTGCTCCTTAGGCGCGTGGGTGGTGCTTTTAGGCCAGGTGTTTTGACAGCTCTAATGGGAGTGAGTGGGGCTGGTAAAACAACTCTAATGGACGTACTGGCTGGTATAAAAACTGGTGGATATATTGAGGGAAATATCACAATTTCCGGGTACCATAAAAAGCAAGAATCGTTTGCCCGGATTTCTGGATATGACATGTTCGTTGAGGAAGTGATGGGACTTGTGGAGCTGAATCCGTTGAGGCAAGCATTAGTAGGGCTGACCATTGCGGTTGAACTAGTGGCTAACCCCTCCATAATATTCATGGATGAACTGACTTCAGGCTTGGATGCAAGTGCTGCGGCCATTGTTATGAGAGCAGTTAGGAATTTGGTTGATAGTGGATTATTGGGTGAGCTCCTACTGCTCCGAGGACGTACTCCAGTTACACTGACTGTTGAGGAACCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

461

Amino Acids

51.27

Weight (kDa)

5.68

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1080
AccB1I GGYRCC 2 cut(s) 304, 1080
AccII CGCG 2 cut(s) 714, 945
AciI CCGC 3 cut(s) 341, 1199, 1271
AcoI YGGCCR 1 cut(s) 1272
AcsI RAATTY 2 cut(s) 389, 1297
AcuI CTGAAG 1 cut(s) 1233
AdeI CACNNNGTG 1 cut(s) 358
AfaI GTAC 5 cut(s) 362, 840, 1026, 1082, 1348
AfiI CCNNNNNNNGG 2 cut(s) 347, 460
AflIII ACRYGT 1 cut(s) 1125
AgsI TTSAA 5 cut(s) 389, 586, 646, 781, 1205
AhlI ACTAGT 1 cut(s) 1207
AjiI CACGTC 1 cut(s) 883
AjnI CCWGG 1 cut(s) 964
AluBI AGCT 7 cut(s) 141, 539, 590, 649, 980, 1159, 1327
AluI AGCT 7 cut(s) 141, 539, 590, 649, 980, 1159, 1327
Alw21I GWGCWC 2 cut(s) 936, 1329
Alw26I GTCTC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 917
AoxI GGCC 3 cut(s) 522, 962, 1272
ApeKI GCWGC 2 cut(s) 539, 1268
ApoI RAATTY 2 cut(s) 389, 1297
AseI ATTAAT 1 cut(s) 9
Asp700I GAANNNNTTC 1 cut(s) 912
Asp718I GGTACC 1 cut(s) 1080
AspLEI GCGC 2 cut(s) 209, 945
AspS9I GGNCC 3 cut(s) 266, 368, 451
AsuC2I CCSGG 2 cut(s) 1078, 1108
AsuHPI GGTGA 6 cut(s) 56, 136, 227, 442, 844, 1334
AsuII TTCGAA 1 cut(s) 396
AvaI CYCGRG 1 cut(s) 917
AvaII GGWCC 3 cut(s) 266, 368, 451
AxyI CCTNAGG 1 cut(s) 938
BanI GGYRCC 2 cut(s) 304, 1080
BanII GRGCYC 1 cut(s) 1329
BauI CACGAG 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 936, 1329
BbvI GCAGC 2 cut(s) 526, 1255
BccI CCATC 2 cut(s) 857, 1138
BceAI ACGGC 1 cut(s) 318
BcgI CGANNNNNNTGC 2 cut(s) 780, 814
BciT130I CCWGG 1 cut(s) 966
BcnI CCSGG 2 cut(s) 1078, 1108
BcoDI GTCTC 1 cut(s) 305
BcuI ACTAGT 1 cut(s) 1207
BfaI CTAG 3 cut(s) 53, 180, 1208
BglII AGATCT 1 cut(s) 793
BisI GCNGC 3 cut(s) 540, 1269, 1272
BlsI GCNGC 3 cut(s) 541, 1270, 1273
Bme1390I CCNGG 3 cut(s) 966, 1078, 1108
Bme18I GGWCC 3 cut(s) 266, 368, 451
BmeT110I CYCGRG 1 cut(s) 917
BmgBI CACGTC 1 cut(s) 883
BmgT120I GGNCC 3 cut(s) 266, 368, 451
BmiI GGNNCC 4 cut(s) 306, 818, 1082, 1374
BmrFI CCNGG 3 cut(s) 966, 1078, 1108
BmsI GCATC 7 cut(s) 88, 198, 239, 246, 696, 718, 1249
BpmI CTGGAG 1 cut(s) 1335
Bpu10I CCTNAGC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 2 cut(s) 1078, 1108
BsaAI YACGTR 1 cut(s) 118
BsaJI CCNNGG 4 cut(s) 364, 762, 918, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 460
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 7 cut(s) 454, 748, 792, 933, 1032, 1048, 1352
Bse21I CCTNAGG 1 cut(s) 938
Bse3DI GCAATG 1 cut(s) 1194
BseBI CCWGG 1 cut(s) 966
BseDI CCNNGG 4 cut(s) 364, 762, 918, 1339
BseGI GGATG 4 cut(s) 48, 103, 1243, 1264
BseLI CCNNNNNNNGG 2 cut(s) 347, 460
BseMI GCAATG 1 cut(s) 1194
BseMII CTCAG 2 cut(s) 300, 693
BseNI ACTGG 7 cut(s) 454, 748, 792, 933, 1032, 1048, 1352
BseXI GCAGC 2 cut(s) 526, 1255
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 2 cut(s) 714, 945
BshFI GGCC 3 cut(s) 524, 964, 1274
BshNI GGYRCC 2 cut(s) 304, 1080
BsiHKAI GWGCWC 2 cut(s) 936, 1329
BsiHKCI CYCGRG 1 cut(s) 917
BsiSI CCGG 4 cut(s) 95, 269, 1077, 1108
BslFI GGGAC 2 cut(s) 401, 1161
BslI CCNNNNNNNGG 2 cut(s) 347, 460
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 2 cut(s) 401, 1161
BsnI GGCC 3 cut(s) 524, 964, 1274
BsoBI CYCGRG 1 cut(s) 917
Bsp119I TTCGAA 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 936, 1329
Bsp143I GATC 2 cut(s) 445, 793
Bsp19I CCATGG 1 cut(s) 762
Bsp68I TCGCGA 1 cut(s) 714
BspACI CCGC 3 cut(s) 341, 1199, 1271
BspANI GGCC 3 cut(s) 524, 964, 1274
BspCNI CTCAG 2 cut(s) 301, 694
BspFNI CGCG 2 cut(s) 714, 945
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 4 cut(s) 306, 818, 1082, 1374
BspT104I TTCGAA 1 cut(s) 396
BspT107I GGYRCC 2 cut(s) 304, 1080
BsrDI GCAATG 1 cut(s) 1194
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 7 cut(s) 454, 748, 792, 933, 1032, 1048, 1352
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 4 cut(s) 364, 762, 918, 1339
BssMI GATC 2 cut(s) 445, 793
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 2 cut(s) 364, 762
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 966
Bst4CI ACNGT 3 cut(s) 582, 808, 1366
Bst6I CTCTTC 1 cut(s) 614
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 396
BstC8I GCNNGC 4 cut(s) 466, 592, 651, 1176
BstDEI CTNAG 6 cut(s) 145, 155, 273, 309, 702, 938
BstDSI CCRYGG 1 cut(s) 762
BstF5I GGATG 4 cut(s) 48, 103, 1243, 1264
BstFNI CGCG 2 cut(s) 714, 945
BstHHI GCGC 2 cut(s) 209, 945
BstKTI GATC 2 cut(s) 448, 796
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 2 cut(s) 445, 793
BstMWI GCNNNNNNNGC 4 cut(s) 596, 940, 1268, 1333
BstNI CCWGG 1 cut(s) 966
BstNSI RCATGY 2 cut(s) 830, 1129
BstSCI CCNGG 3 cut(s) 964, 1076, 1106
BstUI CGCG 2 cut(s) 714, 945
BstV1I GCAGC 2 cut(s) 526, 1255
BstX2I RGATCY 1 cut(s) 793
BstYI RGATCY 1 cut(s) 793
Bsu36I CCTNAGG 1 cut(s) 938
BsuRI GGCC 3 cut(s) 524, 964, 1274
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 695
BtrI CACGTC 1 cut(s) 883
BtsCI GGATG 4 cut(s) 48, 103, 1243, 1264
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 190, 1358
BtuMI TCGCGA 1 cut(s) 714
Cac8I GCNNGC 4 cut(s) 466, 592, 651, 1176
CciI TCATGA 1 cut(s) 615
CfoI GCGC 2 cut(s) 209, 945
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 3 cut(s) 266, 368, 451
Csp6I GTAC 5 cut(s) 361, 839, 1025, 1081, 1347
CviAII CATG 9 cut(s) 286, 402, 497, 616, 667, 763, 827, 1126, 1234
CviQI GTAC 5 cut(s) 361, 839, 1025, 1081, 1347
DdeI CTNAG 6 cut(s) 145, 155, 273, 309, 702, 938
DpnI GATC 2 cut(s) 447, 795
DpnII GATC 2 cut(s) 445, 793
DraI TTTAAA 1 cut(s) 36
DraIII CACNNNGTG 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 1272
Eam1104I CTCTTC 1 cut(s) 614
EarI CTCTTC 1 cut(s) 614
Ecl136II GAGCTC 1 cut(s) 1327
Eco130I CCWWGG 2 cut(s) 364, 762
Eco24I GRGCYC 1 cut(s) 1329
Eco32I GATATC 2 cut(s) 112, 161
Eco47I GGWCC 3 cut(s) 266, 368, 451
Eco53kI GAGCTC 1 cut(s) 1327
Eco57I CTGAAG 1 cut(s) 1233
Eco81I CCTNAGG 1 cut(s) 938
Eco88I CYCGRG 1 cut(s) 917
EcoICRI GAGCTC 1 cut(s) 1327
EcoO109I RGGNCCY 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 964
EcoRV GATATC 2 cut(s) 112, 161
EcoT14I CCWWGG 2 cut(s) 364, 762
EcoT22I ATGCAT 1 cut(s) 711
EcoT38I GRGCYC 1 cut(s) 1329
ErhI CCWWGG 2 cut(s) 364, 762
FaeI CATG 9 cut(s) 289, 405, 500, 619, 670, 766, 830, 1129, 1237
FaqI GGGAC 2 cut(s) 401, 1161
FatI CATG 9 cut(s) 285, 401, 496, 615, 666, 762, 826, 1125, 1233
Fnu4HI GCNGC 3 cut(s) 540, 1269, 1272
FokI GGATG 4 cut(s) 35, 110, 1250, 1271
FriOI GRGCYC 1 cut(s) 1329
Fsp4HI GCNGC 3 cut(s) 540, 1269, 1272
FspBI CTAG 3 cut(s) 53, 180, 1208
GlaI GCGC 2 cut(s) 208, 944
GluI GCNGC 3 cut(s) 540, 1269, 1272
GsaI CCCAGC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 1335
HaeIII GGCC 3 cut(s) 524, 964, 1274
HapII CCGG 4 cut(s) 95, 269, 1077, 1108
HhaI GCGC 2 cut(s) 209, 945
Hin1II CATG 9 cut(s) 289, 405, 500, 619, 670, 766, 830, 1129, 1237
Hin6I GCGC 2 cut(s) 207, 943
HinP1I GCGC 2 cut(s) 207, 943
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 2 cut(s) 588, 647
HinfI GANTC 4 cut(s) 398, 716, 1097, 1162
HpaII CCGG 4 cut(s) 95, 269, 1077, 1108
HphI GGTGA 6 cut(s) 56, 136, 227, 442, 844, 1334
Hpy166II GTNNAC 2 cut(s) 361, 880
Hpy188I TCNGA 3 cut(s) 194, 412, 1340
Hpy188III TCNNGA 8 cut(s) 374, 386, 449, 616, 713, 820, 917, 1116
Hpy8I GTNNAC 2 cut(s) 361, 880
HpyAV CCTTC 3 cut(s) 115, 886, 902
HpyCH4III ACNGT 3 cut(s) 582, 808, 1366
HpyCH4IV ACGT 4 cut(s) 117, 882, 1023, 1345
HpyCH4V TGCA 5 cut(s) 101, 542, 599, 709, 1262
HpyF10VI GCNNNNNNNGC 4 cut(s) 596, 940, 1268, 1333
HpyF3I CTNAG 6 cut(s) 145, 155, 273, 309, 702, 938
HpySE526I ACGT 4 cut(s) 117, 882, 1023, 1345
Hsp92II CATG 9 cut(s) 289, 405, 500, 619, 670, 766, 830, 1129, 1237
HspAI GCGC 2 cut(s) 207, 943
KpnI GGTACC 1 cut(s) 1084
Kzo9I GATC 2 cut(s) 445, 793
LmnI GCTCC 6 cut(s) 822, 835, 939, 1156, 1332, 1341
Lsp1109I GCAGC 2 cut(s) 526, 1255
LweI GCATC 7 cut(s) 88, 198, 239, 246, 696, 718, 1249
MaeI CTAG 3 cut(s) 53, 180, 1208
MaeII ACGT 4 cut(s) 117, 882, 1023, 1345
MaeIII GTNAC 6 cut(s) 44, 215, 350, 430, 808, 1354
MalI GATC 2 cut(s) 447, 795
MboI GATC 2 cut(s) 445, 793
MboII GAAGA 5 cut(s) 27, 92, 631, 748, 913
MflI RGATCY 1 cut(s) 793
MhlI GDGCHC 2 cut(s) 936, 1329
MluCI AATT 5 cut(s) 37, 389, 507, 1071, 1297
MmeI TCCRAC 1 cut(s) 244
Mph1103I ATGCAT 1 cut(s) 711
MroXI GAANNNNTTC 1 cut(s) 912
MseI TTAA 5 cut(s) 9, 35, 62, 545, 1381
MslI CAYNNNNRTG 2 cut(s) 671, 1280
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 4 cut(s) 95, 269, 1077, 1108
MspR9I CCNGG 3 cut(s) 966, 1078, 1108
MvaI CCWGG 1 cut(s) 966
MvnI CGCG 2 cut(s) 714, 945
MwoI GCNNNNNNNGC 4 cut(s) 596, 940, 1268, 1333
NciI CCSGG 2 cut(s) 1078, 1108
NcoI CCATGG 1 cut(s) 762
NdeII GATC 2 cut(s) 445, 793
NlaIII CATG 9 cut(s) 289, 405, 500, 619, 670, 766, 830, 1129, 1237
NlaIV GGNNCC 4 cut(s) 306, 818, 1082, 1374
NmuCI GTSAC 4 cut(s) 44, 215, 350, 430
NruI TCGCGA 1 cut(s) 714
NsiI ATGCAT 1 cut(s) 711
NspI RCATGY 2 cut(s) 830, 1129
NspV TTCGAA 1 cut(s) 396
PagI TCATGA 1 cut(s) 615
PciI ACATGT 1 cut(s) 1125
PdmI GAANNNNTTC 1 cut(s) 912
PfeI GAWTC 4 cut(s) 398, 716, 1097, 1162
PkrI GCNGC 3 cut(s) 541, 1270, 1273
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 368
PscI ACATGT 1 cut(s) 1125
PshBI ATTAAT 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 1329
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 964
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 1 cut(s) 964
PspN4I GGNNCC 4 cut(s) 306, 818, 1082, 1374
PspPI GGNCC 3 cut(s) 266, 368, 451
PspPPI RGGWCCY 1 cut(s) 368
PsuI RGATCY 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 539
RruI TCGCGA 1 cut(s) 714
RsaI GTAC 5 cut(s) 362, 840, 1026, 1082, 1348
RsaNI GTAC 5 cut(s) 361, 839, 1025, 1081, 1347
RseI CAYNNNNRTG 2 cut(s) 671, 1280
SacI GAGCTC 1 cut(s) 1329
SaqAI TTAA 5 cut(s) 9, 35, 62, 545, 1381
SatI GCNGC 3 cut(s) 540, 1269, 1272
Sau3AI GATC 2 cut(s) 445, 793
Sau96I GGNCC 3 cut(s) 266, 368, 451
ScrFI CCNGG 3 cut(s) 966, 1078, 1108
SduI GDGCHC 2 cut(s) 936, 1329
SfaNI GCATC 7 cut(s) 88, 198, 239, 246, 696, 718, 1249
SfuI TTCGAA 1 cut(s) 396
SinI GGWCC 3 cut(s) 266, 368, 451
SmiMI CAYNNNNRTG 2 cut(s) 671, 1280
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 1207
Sse9I AATT 5 cut(s) 37, 389, 507, 1071, 1297
SsiI CCGC 3 cut(s) 341, 1199, 1271
SspI AATATT 1 cut(s) 1230
SspMI CTAG 3 cut(s) 53, 180, 1208
SstI GAGCTC 1 cut(s) 1329
StyD4I CCNGG 3 cut(s) 964, 1076, 1106
StyI CCWWGG 2 cut(s) 364, 762
TaaI ACNGT 3 cut(s) 582, 808, 1366
TaiI ACGT 4 cut(s) 120, 885, 1026, 1348
TaqI TCGA 3 cut(s) 13, 396, 800
TasI AATT 5 cut(s) 37, 389, 507, 1071, 1297
TauI GCSGC 1 cut(s) 1274
TfiI GAWTC 4 cut(s) 398, 716, 1097, 1162
Tru1I TTAA 5 cut(s) 9, 35, 62, 545, 1381
Tru9I TTAA 5 cut(s) 9, 35, 62, 545, 1381
TscAI CASTG 2 cut(s) 190, 1365
TseFI GTSAC 4 cut(s) 44, 215, 350, 430
TseI GCWGC 2 cut(s) 539, 1268
Tsp45I GTSAC 4 cut(s) 44, 215, 350, 430
TspDTI ATGAA 9 cut(s) 274, 478, 513, 632, 876, 914, 921, 1222, 1254
TspGWI ACGGA 3 cut(s) 822, 865, 1155
TspRI CASTG 2 cut(s) 190, 1365
VpaK11BI GGWCC 3 cut(s) 266, 368, 451
VspI ATTAAT 1 cut(s) 9
XapI RAATTY 2 cut(s) 389, 1297
XceI RCATGY 2 cut(s) 830, 1129
XcmI CCANNNNNNNNNTGG 1 cut(s) 1231
XmnI GAANNNNTTC 1 cut(s) 912
XspI CTAG 3 cut(s) 53, 180, 1208
Zsp2I ATGCAT 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.