Belongs to the SKP1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 17, 80, 311, 365 |
| AcoI | YGGCCR | 1 cut(s) | 84 |
| AdeI | CACNNNGTG | 1 cut(s) | 115 |
| AfaI | GTAC | 1 cut(s) | 191 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 178, 307 |
| AflIII | ACRYGT | 1 cut(s) | 350 |
| AgsI | TTSAA | 2 cut(s) | 25, 412 |
| AjiI | CACGTC | 1 cut(s) | 351 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 251, 283 |
| AluBI | AGCT | 1 cut(s) | 48 |
| AluI | AGCT | 1 cut(s) | 48 |
| Alw21I | GWGCWC | 1 cut(s) | 50 |
| Ama87I | CYCGRG | 1 cut(s) | 434 |
| AoxI | GGCC | 3 cut(s) | 3, 84, 334 |
| ApeKI | GCWGC | 1 cut(s) | 227 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 47, 79 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 396, 425, 450 |
| AspLEI | GCGC | 1 cut(s) | 167 |
| AspS9I | GGNCC | 1 cut(s) | 334 |
| AvaI | CYCGRG | 1 cut(s) | 434 |
| BalI | TGGCCA | 1 cut(s) | 86 |
| BanII | GRGCYC | 1 cut(s) | 50 |
| BbsI | GAAGAC | 1 cut(s) | 389 |
| Bbv12I | GWGCWC | 1 cut(s) | 50 |
| BbvI | GCAGC | 1 cut(s) | 214 |
| BccI | CCATC | 2 cut(s) | 110, 176 |
| BceAI | ACGGC | 1 cut(s) | 250 |
| BclI | TGATCA | 1 cut(s) | 372 |
| BfmI | CTRYAG | 1 cut(s) | 193 |
| BfoI | RGCGCY | 1 cut(s) | 168 |
| BisI | GCNGC | 2 cut(s) | 228, 312 |
| BlsI | GCNGC | 2 cut(s) | 229, 313 |
| BmcAI | AGTACT | 1 cut(s) | 191 |
| BmeT110I | CYCGRG | 1 cut(s) | 434 |
| BmgBI | CACGTC | 1 cut(s) | 351 |
| BmgT120I | GGNCC | 1 cut(s) | 334 |
| BmsI | GCATC | 2 cut(s) | 211, 250 |
| BpiI | GAAGAC | 1 cut(s) | 389 |
| BsaJI | CCNNGG | 2 cut(s) | 87, 435 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 452, 482 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 178, 307 |
| Bse118I | RCCGGY | 1 cut(s) | 131 |
| Bse1I | ACTGG | 1 cut(s) | 466 |
| Bse3DI | GCAATG | 1 cut(s) | 142 |
| BseDI | CCNNGG | 2 cut(s) | 87, 435 |
| BseGI | GGATG | 3 cut(s) | 131, 168, 265 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 178, 307 |
| BseMI | GCAATG | 1 cut(s) | 142 |
| BseNI | ACTGG | 1 cut(s) | 466 |
| BseXI | GCAGC | 1 cut(s) | 214 |
| BshFI | GGCC | 3 cut(s) | 5, 86, 336 |
| BsiHKAI | GWGCWC | 1 cut(s) | 50 |
| BsiHKCI | CYCGRG | 1 cut(s) | 434 |
| BsiSI | CCGG | 1 cut(s) | 132 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 178, 307 |
| BsnI | GGCC | 3 cut(s) | 5, 86, 336 |
| BsoBI | CYCGRG | 1 cut(s) | 434 |
| Bsp1286I | GDGCHC | 1 cut(s) | 50 |
| Bsp143I | GATC | 2 cut(s) | 276, 372 |
| Bsp19I | CCATGG | 1 cut(s) | 87 |
| BspACI | CCGC | 4 cut(s) | 17, 80, 311, 365 |
| BspANI | GGCC | 3 cut(s) | 5, 86, 336 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspMAI | CTGCAG | 1 cut(s) | 197 |
| BsrDI | GCAATG | 1 cut(s) | 142 |
| BsrFI | RCCGGY | 1 cut(s) | 131 |
| BsrI | ACTGG | 1 cut(s) | 466 |
| BssAI | RCCGGY | 1 cut(s) | 131 |
| BssECI | CCNNGG | 2 cut(s) | 87, 435 |
| BssMI | GATC | 2 cut(s) | 276, 372 |
| BssT1I | CCWWGG | 1 cut(s) | 87 |
| Bst4CI | ACNGT | 1 cut(s) | 361 |
| BstC8I | GCNNGC | 2 cut(s) | 133, 338 |
| BstDSI | CCRYGG | 1 cut(s) | 87 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 176 |
| BstF5I | GGATG | 3 cut(s) | 131, 168, 265 |
| BstH2I | RGCGCY | 1 cut(s) | 168 |
| BstHHI | GCGC | 1 cut(s) | 167 |
| BstKTI | GATC | 2 cut(s) | 279, 375 |
| BstMBI | GATC | 2 cut(s) | 276, 372 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 137, 173, 227 |
| BstSFI | CTRYAG | 1 cut(s) | 193 |
| BstV1I | GCAGC | 1 cut(s) | 214 |
| BstV2I | GAAGAC | 1 cut(s) | 389 |
| BsuRI | GGCC | 3 cut(s) | 5, 86, 336 |
| BtgI | CCRYGG | 1 cut(s) | 87 |
| BtrI | CACGTC | 1 cut(s) | 351 |
| BtsCI | GGATG | 3 cut(s) | 131, 168, 265 |
| BtsIMutI | CAGTG | 1 cut(s) | 473 |
| Cac8I | GCNNGC | 2 cut(s) | 133, 338 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 1 cut(s) | 167 |
| Cfr10I | RCCGGY | 1 cut(s) | 131 |
| Cfr13I | GGNCC | 1 cut(s) | 334 |
| Csp6I | GTAC | 1 cut(s) | 190 |
| CviAII | CATG | 4 cut(s) | 88, 112, 271, 370 |
| CviJI | RGCY | 6 cut(s) | 5, 48, 86, 221, 336, 473 |
| CviKI_1 | RGCY | 6 cut(s) | 5, 48, 86, 221, 336, 473 |
| CviQI | GTAC | 1 cut(s) | 190 |
| DpnI | GATC | 2 cut(s) | 278, 374 |
| DpnII | GATC | 2 cut(s) | 276, 372 |
| DraIII | CACNNNGTG | 1 cut(s) | 115 |
| EaeI | YGGCCR | 1 cut(s) | 84 |
| EciI | GGCGGA | 1 cut(s) | 380 |
| Ecl136II | GAGCTC | 1 cut(s) | 48 |
| Eco130I | CCWWGG | 1 cut(s) | 87 |
| Eco24I | GRGCYC | 1 cut(s) | 50 |
| Eco53kI | GAGCTC | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 1 cut(s) | 434 |
| EcoICRI | GAGCTC | 1 cut(s) | 48 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 176 |
| EcoO109I | RGGNCCY | 1 cut(s) | 334 |
| EcoT14I | CCWWGG | 1 cut(s) | 87 |
| EcoT38I | GRGCYC | 1 cut(s) | 50 |
| ErhI | CCWWGG | 1 cut(s) | 87 |
| FaeI | CATG | 4 cut(s) | 91, 115, 274, 373 |
| FaiI | YATR | 6 cut(s) | 89, 113, 272, 285, 297, 371 |
| FatI | CATG | 4 cut(s) | 87, 111, 270, 369 |
| FbaI | TGATCA | 1 cut(s) | 372 |
| Fnu4HI | GCNGC | 2 cut(s) | 228, 312 |
| FokI | GGATG | 3 cut(s) | 138, 155, 272 |
| FriOI | GRGCYC | 1 cut(s) | 50 |
| Fsp4HI | GCNGC | 2 cut(s) | 228, 312 |
| GlaI | GCGC | 1 cut(s) | 166 |
| GluI | GCNGC | 2 cut(s) | 228, 312 |
| HaeII | RGCGCY | 1 cut(s) | 168 |
| HaeIII | GGCC | 3 cut(s) | 5, 86, 336 |
| HapII | CCGG | 1 cut(s) | 132 |
| HhaI | GCGC | 1 cut(s) | 167 |
| Hin1II | CATG | 4 cut(s) | 91, 115, 274, 373 |
| Hin6I | GCGC | 1 cut(s) | 165 |
| HinP1I | GCGC | 1 cut(s) | 165 |
| HinfI | GANTC | 3 cut(s) | 92, 397, 451 |
| HpaII | CCGG | 1 cut(s) | 132 |
| Hpy188I | TCNGA | 2 cut(s) | 52, 357 |
| Hpy188III | TCNNGA | 6 cut(s) | 206, 250, 271, 323, 418, 434 |
| Hpy99I | CGWCG | 2 cut(s) | 56, 59 |
| HpyAV | CCTTC | 2 cut(s) | 172, 418 |
| HpyCH4III | ACNGT | 1 cut(s) | 361 |
| HpyCH4IV | ACGT | 1 cut(s) | 350 |
| HpyCH4V | TGCA | 1 cut(s) | 195 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 137, 173, 227 |
| HpySE526I | ACGT | 1 cut(s) | 350 |
| Hsp92II | CATG | 4 cut(s) | 91, 115, 274, 373 |
| HspAI | GCGC | 1 cut(s) | 165 |
| KroI | GCCGGC | 1 cut(s) | 131 |
| KroNI | GCCGGC | 1 cut(s) | 133 |
| Ksp22I | TGATCA | 1 cut(s) | 372 |
| Kzo9I | GATC | 2 cut(s) | 276, 372 |
| LmnI | GCTCC | 1 cut(s) | 53 |
| LpnPI | CCDG | 7 cut(s) | 62, 145, 181, 350, 359, 402, 479 |
| Lsp1109I | GCAGC | 1 cut(s) | 214 |
| LweI | GCATC | 2 cut(s) | 211, 250 |
| MaeII | ACGT | 1 cut(s) | 350 |
| MaeIII | GTNAC | 1 cut(s) | 157 |
| MalI | GATC | 2 cut(s) | 278, 374 |
| MboI | GATC | 2 cut(s) | 276, 372 |
| MboII | GAAGA | 6 cut(s) | 43, 221, 286, 394, 452, 455 |
| MhlI | GDGCHC | 1 cut(s) | 50 |
| MlsI | TGGCCA | 1 cut(s) | 86 |
| MluNI | TGGCCA | 1 cut(s) | 86 |
| MlyI | GAGTC | 1 cut(s) | 101 |
| MmeI | TCCRAC | 1 cut(s) | 75 |
| MnlI | CCTC | 5 cut(s) | 16, 61, 112, 208, 430 |
| Mox20I | TGGCCA | 1 cut(s) | 86 |
| MroNI | GCCGGC | 1 cut(s) | 131 |
| MroXI | GAANNNNTTC | 3 cut(s) | 396, 425, 450 |
| MscI | TGGCCA | 1 cut(s) | 86 |
| Msp20I | TGGCCA | 1 cut(s) | 86 |
| MspA1I | CMGCKG | 1 cut(s) | 17 |
| MspI | CCGG | 1 cut(s) | 132 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 137, 173, 227 |
| NaeI | GCCGGC | 1 cut(s) | 133 |
| NcoI | CCATGG | 1 cut(s) | 87 |
| NdeII | GATC | 2 cut(s) | 276, 372 |
| NgoMIV | GCCGGC | 1 cut(s) | 131 |
| NlaIII | CATG | 4 cut(s) | 91, 115, 274, 373 |
| NmeAIII | GCCGAG | 1 cut(s) | 289 |
| NmuCI | GTSAC | 1 cut(s) | 157 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 63 |
| PdiI | GCCGGC | 1 cut(s) | 133 |
| PdmI | GAANNNNTTC | 3 cut(s) | 396, 425, 450 |
| PfeI | GAWTC | 2 cut(s) | 397, 451 |
| PkrI | GCNGC | 2 cut(s) | 229, 313 |
| PleI | GAGTC | 1 cut(s) | 100 |
| PpsI | GAGTC | 1 cut(s) | 100 |
| Psp124BI | GAGCTC | 1 cut(s) | 50 |
| PspPI | GGNCC | 1 cut(s) | 334 |
| PstI | CTGCAG | 1 cut(s) | 197 |
| RsaI | GTAC | 1 cut(s) | 191 |
| RsaNI | GTAC | 1 cut(s) | 190 |
| SacI | GAGCTC | 1 cut(s) | 50 |
| SatI | GCNGC | 2 cut(s) | 228, 312 |
| Sau3AI | GATC | 2 cut(s) | 276, 372 |
| Sau96I | GGNCC | 1 cut(s) | 334 |
| ScaI | AGTACT | 1 cut(s) | 191 |
| SchI | GAGTC | 1 cut(s) | 101 |
| SduI | GDGCHC | 1 cut(s) | 50 |
| SetI | ASST | 8 cut(s) | 50, 72, 183, 303, 348, 353, 391, 410 |
| SfaNI | GCATC | 2 cut(s) | 211, 250 |
| SfcI | CTRYAG | 1 cut(s) | 193 |
| SsiI | CCGC | 4 cut(s) | 17, 80, 311, 365 |
| SstI | GAGCTC | 1 cut(s) | 50 |
| StyI | CCWWGG | 1 cut(s) | 87 |
| TaaI | ACNGT | 1 cut(s) | 361 |
| TaiI | ACGT | 1 cut(s) | 353 |
| TaqI | TCGA | 4 cut(s) | 66, 279, 324, 477 |
| TatI | WGTACW | 1 cut(s) | 189 |
| TauI | GCSGC | 1 cut(s) | 314 |
| TfiI | GAWTC | 2 cut(s) | 397, 451 |
| TscAI | CASTG | 1 cut(s) | 473 |
| TseFI | GTSAC | 1 cut(s) | 157 |
| TseI | GCWGC | 1 cut(s) | 227 |
| Tsp45I | GTSAC | 1 cut(s) | 157 |
| TspDTI | ATGAA | 2 cut(s) | 259, 287 |
| TspGWI | ACGGA | 1 cut(s) | 138 |
| TspRI | CASTG | 1 cut(s) | 473 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 176 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 109 |
| XmnI | GAANNNNTTC | 3 cut(s) | 396, 425, 450 |
| ZrmI | AGTACT | 1 cut(s) | 191 |