pycom16g14880

1-deoxy-D-xylulose-5-phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000901
Physical Location & Seq
Reverse (-)
10886467 .. 10890057
3591 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1896 bp
ATGGCTCTCTGCGCATTCTCATTTCCTGCACATATCAGCAAACCAGTAGCATCTGCAGATGCTCAGAAATCCCACTTCTCTGTGGGAACACCTCTTCCATGCCCATCTCAGCAGAAGCTCAATAAGGTGATGAGAAAACGGCCAAGTGGGATTTGTGCATCACTGTCAGAGAGAGGGGAGTATCACTCACAGAGACCTCCCACTCCTCTCTTGGACACCATCAACTATCCAATTCACATGAAAAATCTTTCTGTCAAGGAGCTTCAACAACTAGCAGATGAGCTGAGGTCTGATGTGATCTTCAATGTTTCAAAAACTGGAGGCCACCTCGGTTCAAGCCTTGGTGTTGTGGAGCCCTCTGTGGCTCTTCACTATGTCTTCAATGCTCCTCAGGACAGAATCTTGTGGGATGTTGGCCATCAGTCTTACCCACACAAAATCTTGACTGGGAGGAGAGAAAAGATGCACACCATGAGACAGACAAATGGATTAGCCGGCTTCACTAAGCGCTCTGAGAGCGAATACGATTGCTTTGGCACTGGTCACAGTTCTACCACTATCTCTGCAGGCTTGGGGATGGCAGTAGGCAGGGATCTAAAGGGAGGAAACAATCATGTAGTTGCTGTAATAGGTGATGGTGCCATGACAGCAGGGCAAGCGTATGAAGCCATGAACAATGCGGGGTACCTCGATTCTGACATGATTATTATTCTTAATGACAATAAACAGGTTTCTTTACCTACCGCTAGTCTTGACGGACCCATCCCGCCGGTAGGAGCTTTGAGTAGTGCTCTCAGTAGGTTGCAATCAAACAGGCCGCTCAGAGAATTAAGAGAGGTCGCCAAAGGAGTTACTAAACAAATTGGTGGCCCTGTGCATAATCTGGCAGCGAAAGTTGACGAGTATGCTCGAGGAATGATCAATGGTACTGAAAAAGCGGCAGACAAGTACCATGGAGTGGCCAAATTTGATCCGGCAACTGGAAAGCAATTCAAAGCAAGTGCTAGTACACAATCTTACACAACATACTTTGCAGAGGCTTTGATTGCAGAAGCTGAAGCGGACAAAGATATTGTTGCTATCCATGCTGCAATGGGAGGTGGAACAGGGATGAATCTCTTCCTCCGCCGGTTTCCAACAAGATGCTTTGATGTTGGAATAGCAGAACAGCATGCGGTTACTTTTGCTGCTGGTTTGGCCTGTGAAGGCCTAAAACCTTTCTGTGCAATTTATTCATCTTTCTTGCAGAGAGCTTATGACCAGGTAGTACATGACGTAGATTTGCAAAAGCTTCCGGTTAGATTTGCTATGGACAGAGCTGGGCTGGTCGGAGCTGATGGTCCTACGCATTCCGGGTCTTTTGATGTCACTTTCATGGCATGCCTTCCTAACATGGTGGTGATGGCCCCTTCTGATGAGGCAGAGCTTTTCGATATGGTAGCCACTGCTGCTGCCATAGATGACCGACCAAGTTGTTTCCGATACCCAAGAGGAAATGGGGTTGGTGTTGAGCTGCCACTAGGGAATAAAGGCACTCCTCTCGAGGTAGGAAAAGGAAGAATATTGATCGAAGGGGAAAGAGTTGCACTTTTGGGTTATGGAGCAGCAGTACAGAGCTGTTTGGCTGCCTCCTCTTTGGTGGAATCCCATGGTTTTCGCCTTACAGTTGCTGATGCACGATTTTGCAAACCACTGGACCGAGCTCTCATTCGCAGCCTTGCGAAGTCACATGAGTTTCTGATAACTGTGGAAGAAGGATCCATCGGAGGGTTTGGTTCTCACGTTGCTCATTTCCTGGCCCTTGATGGCCTTCTTGATGGAAAACTCAAGTGGAGACCGATTGTTCTTCCTGACCGGTACATTGACCACGGTTCCCCTGATCGATCAATTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

632

Amino Acids

67.69

Weight (kDa)

7.33

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 71 - 309 7.5e-90 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 338 - 499 1.7e-49 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 517 - 627 3e-26 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 13
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 2 cut(s) 638, 684
AccB7I CCANNNNNTGG 1 cut(s) 958
AccBSI CCGCTC 1 cut(s) 820
AciI CCGC 8 cut(s) 680, 744, 767, 818, 938, 1061, 1126, 1175
AclWI GGATC 4 cut(s) 600, 965, 1752, 1765
AcoI YGGCCR 3 cut(s) 140, 415, 960
AcsI RAATTY 1 cut(s) 965
AcuI CTGAAG 1 cut(s) 1077
AfaI GTAC 7 cut(s) 686, 928, 950, 1009, 1269, 1611, 1859
AfeI AGCGCT 1 cut(s) 509
AfiI CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
AgeI ACCGGT 1 cut(s) 1854
AgsI TTSAA 6 cut(s) 266, 304, 312, 336, 382, 994
AjnI CCWGG 2 cut(s) 1260, 1794
AloI GAACNNNNNNTCC 2 cut(s) 79, 111
Alw21I GWGCWC 2 cut(s) 793, 1705
Alw26I GTCTC 3 cut(s) 187, 469, 1828
AlwI GGATC 4 cut(s) 600, 965, 1752, 1765
AlwNI CAGNNNCTG 2 cut(s) 1055, 1670
Ama87I CYCGRG 2 cut(s) 909, 1541
Aor51HI AGCGCT 1 cut(s) 509
ApeKI GCWGC 9 cut(s) 887, 1088, 1187, 1448, 1451, 1513, 1604, 1625, 1713
ApoI RAATTY 1 cut(s) 965
AsiGI ACCGGT 1 cut(s) 1854
Asp700I GAANNNNTTC 1 cut(s) 1118
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 2 cut(s) 14, 510
AspS9I GGNCC 6 cut(s) 758, 869, 1340, 1405, 1696, 1798
AsuC2I CCSGG 1 cut(s) 1354
AsuHPI GGTGA 3 cut(s) 139, 644, 1411
AvaI CYCGRG 2 cut(s) 909, 1541
AvaII GGWCC 3 cut(s) 758, 1340, 1696
AxyI CCTNAGG 1 cut(s) 390
BalI TGGCCA 2 cut(s) 417, 962
BamHI GGATCC 1 cut(s) 1757
BanI GGYRCC 2 cut(s) 638, 684
BanII GRGCYC 2 cut(s) 357, 1705
BbsI GAAGAC 1 cut(s) 370
Bbv12I GWGCWC 2 cut(s) 793, 1705
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 9 cut(s) 899, 1075, 1174, 1435, 1438, 1500, 1612, 1616, 1725
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 1522, 1556
BciT130I CCWGG 2 cut(s) 1262, 1796
BclI TGATCA 1 cut(s) 918
BcnI CCSGG 1 cut(s) 1354
BcoDI GTCTC 3 cut(s) 187, 469, 1828
BfaI CTAG 4 cut(s) 272, 747, 1005, 1520
BfmI CTRYAG 2 cut(s) 54, 564
BfoI RGCGCY 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 1262, 1354, 1796
Bme18I GGWCC 3 cut(s) 758, 1340, 1696
BmeT110I CYCGRG 2 cut(s) 909, 1541
BmgT120I GGNCC 6 cut(s) 758, 869, 1340, 1405, 1696, 1798
BmiI GGNNCC 7 cut(s) 354, 640, 686, 760, 1407, 1759, 1873
BmrFI CCNGG 3 cut(s) 1262, 1354, 1796
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 6 cut(s) 49, 59, 167, 453, 1133, 1663
BmuI ACTGGG 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 370
BplI GAGNNNNNCTC 6 cut(s) 170, 202, 312, 344, 775, 807
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 1811
BpuMI CCSGG 1 cut(s) 1354
Bsa29I ATCGAT 1 cut(s) 1882
BsaI GGTCTC 2 cut(s) 187, 1828
BsaJI CCNNGG 5 cut(s) 328, 340, 952, 1648, 1867
BsaWI WCCGGW 2 cut(s) 1294, 1854
BsaXI ACNNNNNCTCC 2 cut(s) 1593, 1623
Bsc4I CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
Bse118I RCCGGY 4 cut(s) 494, 769, 1128, 1854
Bse1I ACTGG 6 cut(s) 44, 322, 451, 544, 985, 1698
Bse21I CCTNAGG 1 cut(s) 390
Bse3DI GCAATG 1 cut(s) 1098
BseBI CCWGG 2 cut(s) 1262, 1796
BseCI ATCGAT 1 cut(s) 1882
BseDI CCNNGG 5 cut(s) 328, 340, 952, 1648, 1867
BseGI GGATG 4 cut(s) 415, 582, 762, 1116
BseLI CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 7 cut(s) 77, 122, 275, 404, 504, 808, 835
BseNI ACTGG 6 cut(s) 44, 322, 451, 544, 985, 1698
BseRI GAGGAG 5 cut(s) 195, 378, 466, 1527, 1621
BseXI GCAGC 9 cut(s) 899, 1075, 1174, 1435, 1438, 1500, 1612, 1616, 1725
BseYI CCCAGC 1 cut(s) 1319
BsgI GTGCAG 1 cut(s) 12
BshNI GGYRCC 2 cut(s) 638, 684
BshTI ACCGGT 1 cut(s) 1854
BshVI ATCGAT 1 cut(s) 1882
BsiHKAI GWGCWC 2 cut(s) 793, 1705
BsiHKCI CYCGRG 2 cut(s) 909, 1541
BsiSI CCGG 7 cut(s) 495, 770, 974, 1129, 1295, 1353, 1855
BslI CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
BsmAI GTCTC 3 cut(s) 187, 469, 1828
BsmI GAATGC 2 cut(s) 14, 1348
Bso31I GGTCTC 2 cut(s) 187, 1828
BsoBI CYCGRG 2 cut(s) 909, 1541
Bsp1286I GDGCHC 3 cut(s) 357, 793, 1705
Bsp143I GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
Bsp19I CCATGG 2 cut(s) 952, 1648
BspACI CCGC 8 cut(s) 680, 744, 767, 818, 938, 1061, 1126, 1175
BspCNI CTCAG 7 cut(s) 76, 121, 276, 403, 505, 807, 834
BspDI ATCGAT 1 cut(s) 1882
BspLI GGNNCC 7 cut(s) 354, 640, 686, 760, 1407, 1759, 1873
BspMAI CTGCAG 2 cut(s) 58, 568
BspPI GGATC 4 cut(s) 600, 965, 1752, 1765
BspQI GCTCTTC 1 cut(s) 372
BspT107I GGYRCC 2 cut(s) 638, 684
BspTNI GGTCTC 2 cut(s) 187, 1828
BsrBI CCGCTC 1 cut(s) 820
BsrDI GCAATG 1 cut(s) 1098
BsrFI RCCGGY 4 cut(s) 494, 769, 1128, 1854
BsrI ACTGG 6 cut(s) 44, 322, 451, 544, 985, 1698
BssAI RCCGGY 4 cut(s) 494, 769, 1128, 1854
BssECI CCNNGG 5 cut(s) 328, 340, 952, 1648, 1867
BssMI GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
BssT1I CCWWGG 3 cut(s) 340, 952, 1648
Bst2UI CCWGG 2 cut(s) 1262, 1796
Bst4CI ACNGT 5 cut(s) 165, 548, 1666, 1747, 1871
Bst6I CTCTTC 3 cut(s) 99, 372, 1124
BstC8I GCNNGC 5 cut(s) 496, 568, 657, 1173, 1381
BstDEI CTNAG 8 cut(s) 63, 108, 284, 390, 504, 513, 794, 821
BstDSI CCRYGG 3 cut(s) 952, 1648, 1867
BstF5I GGATG 4 cut(s) 415, 582, 762, 1116
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 2 cut(s) 14, 510
BstKTI GATC 8 cut(s) 300, 595, 921, 973, 1569, 1760, 1882, 1886
BstMAI GTCTC 3 cut(s) 187, 469, 1828
BstMBI GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
BstMWI GCNNNNNNNGC 9 cut(s) 11, 516, 647, 656, 665, 1046, 1085, 1196, 1448
BstNI CCWGG 2 cut(s) 1262, 1796
BstNSI RCATGY 2 cut(s) 1175, 1383
BstSCI CCNGG 3 cut(s) 1260, 1352, 1794
BstSFI CTRYAG 2 cut(s) 54, 564
BstV1I GCAGC 9 cut(s) 899, 1075, 1174, 1435, 1438, 1500, 1612, 1616, 1725
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 2 cut(s) 592, 1757
BstYI RGATCY 2 cut(s) 592, 1757
Bsu15I ATCGAT 1 cut(s) 1882
Bsu36I CCTNAGG 1 cut(s) 390
BsuTUI ATCGAT 1 cut(s) 1882
BtgI CCRYGG 3 cut(s) 952, 1648, 1867
BtsCI GGATG 4 cut(s) 415, 582, 762, 1116
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 4 cut(s) 161, 537, 1443, 1691
Cac8I GCNNGC 5 cut(s) 496, 568, 657, 1173, 1381
CaiI CAGNNNCTG 2 cut(s) 1055, 1670
CfoI GCGC 2 cut(s) 14, 510
Cfr10I RCCGGY 4 cut(s) 494, 769, 1128, 1854
Cfr13I GGNCC 6 cut(s) 758, 869, 1340, 1405, 1696, 1798
ClaI ATCGAT 1 cut(s) 1882
CsiI ACCWGGT 1 cut(s) 1260
Csp6I GTAC 7 cut(s) 685, 927, 949, 1008, 1268, 1610, 1858
CspAI ACCGGT 1 cut(s) 1854
CviQI GTAC 7 cut(s) 685, 927, 949, 1008, 1268, 1610, 1858
DdeI CTNAG 8 cut(s) 63, 108, 284, 390, 504, 513, 794, 821
DpnI GATC 8 cut(s) 299, 594, 920, 972, 1568, 1759, 1881, 1885
DpnII GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
EaeI YGGCCR 3 cut(s) 140, 415, 960
Eam1104I CTCTTC 3 cut(s) 99, 372, 1124
EarI CTCTTC 3 cut(s) 99, 372, 1124
EciI GGCGGA 1 cut(s) 1115
Ecl136II GAGCTC 1 cut(s) 1703
Eco130I CCWWGG 3 cut(s) 340, 952, 1648
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 2 cut(s) 357, 1705
Eco31I GGTCTC 2 cut(s) 187, 1828
Eco47I GGWCC 3 cut(s) 758, 1340, 1696
Eco47III AGCGCT 1 cut(s) 509
Eco53kI GAGCTC 1 cut(s) 1703
Eco57I CTGAAG 1 cut(s) 1077
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 909, 1541
EcoICRI GAGCTC 1 cut(s) 1703
EcoRII CCWGG 2 cut(s) 1260, 1794
EcoT14I CCWWGG 3 cut(s) 340, 952, 1648
EcoT38I GRGCYC 2 cut(s) 357, 1705
ErhI CCWWGG 3 cut(s) 340, 952, 1648
FauI CCCGC 2 cut(s) 673, 774
FbaI TGATCA 1 cut(s) 918
FokI GGATG 4 cut(s) 422, 589, 749, 1123
FriOI GRGCYC 2 cut(s) 357, 1705
FspBI CTAG 4 cut(s) 272, 747, 1005, 1520
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 2 cut(s) 13, 509
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 511
HapII CCGG 7 cut(s) 495, 770, 974, 1129, 1295, 1353, 1855
HhaI GCGC 2 cut(s) 14, 510
Hin6I GCGC 2 cut(s) 12, 508
HinP1I GCGC 2 cut(s) 12, 508
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 1 cut(s) 1289
HinfI GANTC 4 cut(s) 399, 692, 1114, 1643
HpaII CCGG 7 cut(s) 495, 770, 974, 1129, 1295, 1353, 1855
HphI GGTGA 3 cut(s) 139, 644, 1411
Hpy166II GTNNAC 2 cut(s) 898, 1010
Hpy188III TCNNGA 6 cut(s) 392, 442, 752, 1541, 1814, 1850
Hpy8I GTNNAC 2 cut(s) 898, 1010
HpyAV CCTTC 6 cut(s) 1199, 1394, 1419, 1565, 1748, 1820
HpyCH4III ACNGT 5 cut(s) 165, 548, 1666, 1747, 1871
HpyCH4IV ACGT 2 cut(s) 1275, 1782
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 516, 647, 656, 665, 1046, 1085, 1196, 1448
HpyF3I CTNAG 8 cut(s) 63, 108, 284, 390, 504, 513, 794, 821
HpySE526I ACGT 2 cut(s) 1275, 1782
HspAI GCGC 2 cut(s) 12, 508
KpnI GGTACC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 494
KroNI GCCGGC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 6 cut(s) 259, 352, 391, 776, 1331, 1601
Lsp1109I GCAGC 9 cut(s) 899, 1075, 1174, 1435, 1438, 1500, 1612, 1616, 1725
LweI GCATC 6 cut(s) 49, 59, 167, 453, 1133, 1663
MabI ACCWGGT 1 cut(s) 1260
MaeI CTAG 4 cut(s) 272, 747, 1005, 1520
MaeII ACGT 2 cut(s) 1275, 1782
MaeIII GTNAC 5 cut(s) 542, 850, 1177, 1366, 1725
MalI GATC 8 cut(s) 299, 594, 920, 972, 1568, 1759, 1881, 1885
MbiI CCGCTC 1 cut(s) 820
MboI GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
MboII GAAGA 8 cut(s) 86, 292, 359, 370, 1111, 1569, 1763, 1838
MfeI CAATTG 1 cut(s) 1887
MflI RGATCY 2 cut(s) 592, 1757
MhlI GDGCHC 3 cut(s) 357, 793, 1705
MlsI TGGCCA 2 cut(s) 417, 962
MluCI AATT 7 cut(s) 231, 827, 861, 965, 989, 1227, 1887
MluNI TGGCCA 2 cut(s) 417, 962
MmeI TCCRAC 3 cut(s) 1135, 1160, 1309
Mox20I TGGCCA 2 cut(s) 417, 962
MroNI GCCGGC 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 1118
MscI TGGCCA 2 cut(s) 417, 962
MseI TTAA 2 cut(s) 714, 830
MslI CAYNNNNRTG 2 cut(s) 1373, 1397
Msp20I TGGCCA 2 cut(s) 417, 962
MspI CCGG 7 cut(s) 495, 770, 974, 1129, 1295, 1353, 1855
MspR9I CCNGG 3 cut(s) 1262, 1354, 1796
MunI CAATTG 1 cut(s) 1887
Mva1269I GAATGC 2 cut(s) 14, 1348
MvaI CCWGG 2 cut(s) 1262, 1796
MwoI GCNNNNNNNGC 9 cut(s) 11, 516, 647, 656, 665, 1046, 1085, 1196, 1448
NaeI GCCGGC 1 cut(s) 496
NciI CCSGG 1 cut(s) 1354
NcoI CCATGG 2 cut(s) 952, 1648
NdeII GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
NgoMIV GCCGGC 1 cut(s) 494
NlaIV GGNNCC 7 cut(s) 354, 640, 686, 760, 1407, 1759, 1873
NmuCI GTSAC 3 cut(s) 542, 1366, 1725
NsbI TGCGCA 1 cut(s) 13
NspI RCATGY 2 cut(s) 1175, 1383
PaeI GCATGC 2 cut(s) 1175, 1383
PaeR7I CTCGAG 2 cut(s) 909, 1541
PceI AGGCCT 1 cut(s) 1209
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 2 cut(s) 14, 1348
PdiI GCCGGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 4 cut(s) 399, 692, 1114, 1643
PflMI CCANNNNNTGG 1 cut(s) 958
PinAI ACCGGT 1 cut(s) 1854
Psp124BI GAGCTC 1 cut(s) 1705
Psp6I CCWGG 2 cut(s) 1260, 1794
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 2 cut(s) 1260, 1794
PspN4I GGNNCC 7 cut(s) 354, 640, 686, 760, 1407, 1759, 1873
PspPI GGNCC 6 cut(s) 758, 869, 1340, 1405, 1696, 1798
PspXI VCTCGAGB 1 cut(s) 909
PstI CTGCAG 2 cut(s) 58, 568
PstNI CAGNNNCTG 2 cut(s) 1055, 1670
PsuI RGATCY 2 cut(s) 592, 1757
RsaI GTAC 7 cut(s) 686, 928, 950, 1009, 1269, 1611, 1859
RsaNI GTAC 7 cut(s) 685, 927, 949, 1008, 1268, 1610, 1858
RseI CAYNNNNRTG 2 cut(s) 1373, 1397
SacI GAGCTC 1 cut(s) 1705
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 2 cut(s) 714, 830
Sau3AI GATC 8 cut(s) 297, 592, 918, 970, 1566, 1757, 1879, 1883
Sau96I GGNCC 6 cut(s) 758, 869, 1340, 1405, 1696, 1798
ScrFI CCNGG 3 cut(s) 1262, 1354, 1796
SduI GDGCHC 3 cut(s) 357, 793, 1705
SexAI ACCWGGT 1 cut(s) 1260
SfaNI GCATC 6 cut(s) 49, 59, 167, 453, 1133, 1663
SfcI CTRYAG 2 cut(s) 54, 564
Sfr274I CTCGAG 2 cut(s) 909, 1541
SinI GGWCC 3 cut(s) 758, 1340, 1696
SlaI CTCGAG 2 cut(s) 909, 1541
SmiMI CAYNNNNRTG 2 cut(s) 1373, 1397
SmlI CTYRAG 3 cut(s) 909, 1541, 1826
SmoI CTYRAG 3 cut(s) 909, 1541, 1826
SphI GCATGC 2 cut(s) 1175, 1383
Sse9I AATT 7 cut(s) 231, 827, 861, 965, 989, 1227, 1887
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 8 cut(s) 680, 744, 767, 818, 938, 1061, 1126, 1175
SspI AATATT 1 cut(s) 1563
SspMI CTAG 4 cut(s) 272, 747, 1005, 1520
SstI GAGCTC 1 cut(s) 1705
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 3 cut(s) 1260, 1352, 1794
StyI CCWWGG 3 cut(s) 340, 952, 1648
TaaI ACNGT 5 cut(s) 165, 548, 1666, 1747, 1871
TaiI ACGT 2 cut(s) 1278, 1785
TaqI TCGA 6 cut(s) 690, 910, 1431, 1542, 1569, 1882
TaqII GACCGA 3 cut(s) 1479, 1713, 1852
TasI AATT 7 cut(s) 231, 827, 861, 965, 989, 1227, 1887
TatI WGTACW 3 cut(s) 1007, 1267, 1609
TauI GCSGC 2 cut(s) 820, 941
TfiI GAWTC 4 cut(s) 399, 692, 1114, 1643
Tru1I TTAA 2 cut(s) 714, 830
Tru9I TTAA 2 cut(s) 714, 830
TscAI CASTG 4 cut(s) 168, 544, 1450, 1698
TseFI GTSAC 3 cut(s) 542, 1366, 1725
TseI GCWGC 9 cut(s) 887, 1088, 1187, 1448, 1451, 1513, 1604, 1625, 1713
Tsp45I GTSAC 3 cut(s) 542, 1366, 1725
TspDTI ATGAA 6 cut(s) 254, 678, 686, 1127, 1224, 1363
TspGWI ACGGA 1 cut(s) 771
TspRI CASTG 4 cut(s) 168, 544, 1450, 1698
Van91I CCANNNNNTGG 1 cut(s) 958
VpaK11BI GGWCC 3 cut(s) 758, 1340, 1696
XapI RAATTY 1 cut(s) 965
XceI RCATGY 2 cut(s) 1175, 1383
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XhoI CTCGAG 2 cut(s) 909, 1541
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 4 cut(s) 272, 747, 1005, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.