RchiOBHm_Chr2g0151011

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000915
Physical Location & Seq
Reverse (-)
68665912 .. 68668477
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1023 bp
ATGACCATGAAGCTTTCGTTGCTCTCATGTTTGCTCCTTCTATCTGTATCATGCAACAGCGCTAACCAAGTTGGCAACCTCAACAAGTTGATTAAGTCTCACAGAAATCAAAAGCCTCTTCAGTCTGAGTCGCAGAATGGCTTAGATCAGGTTGGAGATAACACTTATTCTCCGGTGTATGTTGGATCCCAAGATGGGTCAAAGGAAGCCGATAAGGTTGAGGCTTTGCCCGGACAACCAACCGAAGGAGTGGATTTTAATCAATATGCAGGATATGTTACGGTTGACGCTGACGCTGGGAGAGCATTGTTCTACTACTTTGTAGAGTCGCCCCAGAACTCTTCAACAAGACCTCTGGTGCTATGGCTAAATGGAGGACCAGGATGCTCGTCTCTTGGATATGGAGCCATGGAAGAACTGGGACCTTTCAGAGTCAACAGCGATGGGAACTCACTATACAAGAATGGTTATGCATGGAACAATGAGGCAAATGTCCTCTTCTTGGAGTCTCCGGCAGGAGTTGGGTTCTCATATTCAAACACAACATCTGACTATCAAAACGTGGGTGACAACAAAACTGCCTCTGACTCTTACAAGTTTCTTATAAACTGGCTAGAAAAATTTCCTGAATACAAAACCAGAGACTTCTTCATCACTGGAGAGAGTTACGCTGGTCACTACGTACCTCAGCTTGCATACACCATTGTCTCTGAGAACAAAATCACAAATCAAACGAAAATCAACCTCAAAGGAATTGCAATTGGGAATGCCTGGATTGATGCCAGTACCGGTACACAGGGTGGTTATGATTATTTGTGGACACATGCTTTGAATTCTGACGAAACCAGTGCAGGAATCAATAAATATTGCGATTTCGTTACTTTTAATATCACAAGCACATGTGATAAATATATGAGTCAGTCAGAGAATGAGGTTGGAGAAATTGATCCTTACAACATATACGCTCCACTTTGCAATACAGAATCCAAACCTGCAGCCTCAACTGTTTCAGTAAGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

340

Amino Acids

37.2

Weight (kDa)

4.62

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 80 - 329 2.9e-97 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
Acc36I ACCTGC 1 cut(s) 1000
AclWI GGATC 3 cut(s) 180, 193, 941
AcsI RAATTY 2 cut(s) 620, 832
AcuI CTGAAG 1 cut(s) 104
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 3 cut(s) 684, 787, 793
AfeI AGCGCT 1 cut(s) 61
AfiI CCNNNNNNNGG 3 cut(s) 195, 245, 502
AflIII ACRYGT 1 cut(s) 899
AgeI ACCGGT 1 cut(s) 788
AgsI TTSAA 3 cut(s) 345, 537, 832
AjnI CCWGG 2 cut(s) 379, 770
AluBI AGCT 2 cut(s) 13, 691
AluI AGCT 2 cut(s) 13, 691
Alw26I GTCTC 5 cut(s) 102, 396, 513, 636, 712
AlwI GGATC 3 cut(s) 180, 193, 941
Aor51HI AGCGCT 1 cut(s) 61
ApeKI GCWGC 1 cut(s) 995
ApoI RAATTY 2 cut(s) 620, 832
ArsI GACNNNNNNTTYG 2 cut(s) 811, 843
AsiGI ACCGGT 1 cut(s) 788
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 377, 422
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 578
AvaII GGWCC 2 cut(s) 377, 422
BamHI GGATCC 1 cut(s) 185
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 1 cut(s) 1007
BccI CCATC 2 cut(s) 188, 437
BcgI CGANNNNNNTGC 2 cut(s) 830, 864
BciT130I CCWGG 2 cut(s) 381, 772
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 5 cut(s) 102, 396, 513, 636, 712
BfaI CTAG 1 cut(s) 614
BfmI CTRYAG 1 cut(s) 993
BfoI RGCGCY 1 cut(s) 63
BfuAI ACCTGC 1 cut(s) 1000
BisI GCNGC 1 cut(s) 996
BlsI GCNGC 1 cut(s) 997
Bme1390I CCNGG 3 cut(s) 231, 381, 772
Bme18I GGWCC 2 cut(s) 377, 422
BmgT120I GGNCC 2 cut(s) 377, 422
BmiI GGNNCC 3 cut(s) 187, 406, 423
BmrFI CCNGG 3 cut(s) 231, 381, 772
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 374, 769
BmuI ACTGGG 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 1 cut(s) 687
BpuMI CCSGG 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 682
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 2 cut(s) 172, 788
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 245, 502
Bse118I RCCGGY 1 cut(s) 788
Bse1I ACTGG 5 cut(s) 423, 614, 661, 783, 846
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 2 cut(s) 381, 772
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 1 cut(s) 389
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 195, 245, 502
BseMII CTCAG 3 cut(s) 117, 701, 702
BseNI ACTGG 5 cut(s) 423, 614, 661, 783, 846
BseXI GCAGC 1 cut(s) 1007
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 870
BshTI ACCGGT 1 cut(s) 788
BsiSI CCGG 4 cut(s) 173, 231, 512, 789
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 3 cut(s) 195, 245, 502
BsmAI GTCTC 5 cut(s) 102, 396, 513, 636, 712
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 772
Bsp143I GATC 3 cut(s) 145, 185, 946
Bsp19I CCATGG 1 cut(s) 408
BspCNI CTCAG 3 cut(s) 118, 700, 703
BspLI GGNNCC 3 cut(s) 187, 406, 423
BspMAI CTGCAG 1 cut(s) 997
BspMI ACCTGC 1 cut(s) 1000
BspPI GGATC 3 cut(s) 180, 193, 941
BsrFI RCCGGY 1 cut(s) 788
BsrI ACTGG 5 cut(s) 423, 614, 661, 783, 846
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 3 cut(s) 145, 185, 946
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 381, 772
Bst4CI ACNGT 2 cut(s) 283, 1006
Bst6I CTCTTC 3 cut(s) 123, 346, 503
BstBAI YACGTR 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 4 cut(s) 126, 142, 687, 711
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 389
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 3 cut(s) 148, 188, 949
BstMAI GTCTC 5 cut(s) 102, 396, 513, 636, 712
BstMBI GATC 3 cut(s) 145, 185, 946
BstMWI GCNNNNNNNGC 2 cut(s) 19, 302
BstNI CCWGG 2 cut(s) 381, 772
BstNSI RCATGY 2 cut(s) 827, 903
BstSCI CCNGG 3 cut(s) 229, 379, 770
BstSFI CTRYAG 1 cut(s) 993
BstSNI TACGTA 1 cut(s) 682
BstV1I GCAGC 1 cut(s) 1007
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 1 cut(s) 389
BtsIMutI CAGTG 2 cut(s) 654, 853
BveI ACCTGC 1 cut(s) 1000
Cac8I GCNNGC 1 cut(s) 693
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 2 cut(s) 377, 422
CseI GACGC 2 cut(s) 296, 302
Csp6I GTAC 3 cut(s) 683, 786, 792
CspAI ACCGGT 1 cut(s) 788
CviAII CATG 7 cut(s) 7, 27, 51, 409, 474, 824, 900
CviQI GTAC 3 cut(s) 683, 786, 792
DdeI CTNAG 4 cut(s) 126, 142, 687, 711
DpnI GATC 3 cut(s) 147, 187, 948
DpnII GATC 3 cut(s) 145, 185, 946
DraIII CACNNNGTG 1 cut(s) 800
Eam1104I CTCTTC 3 cut(s) 123, 346, 503
EarI CTCTTC 3 cut(s) 123, 346, 503
Eco105I TACGTA 1 cut(s) 682
Eco130I CCWWGG 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 377, 422
Eco47III AGCGCT 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 832
EcoRII CCWGG 2 cut(s) 379, 770
EcoT14I CCWWGG 1 cut(s) 408
EcoT22I ATGCAT 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 7 cut(s) 10, 30, 54, 412, 477, 827, 903
FaqI GGGAC 1 cut(s) 435
FatI CATG 7 cut(s) 6, 26, 50, 408, 473, 823, 899
Fnu4HI GCNGC 1 cut(s) 996
FokI GGATG 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 996
FspBI CTAG 1 cut(s) 614
GlaI GCGC 1 cut(s) 61
GluI GCNGC 1 cut(s) 996
GsaI CCCAGC 1 cut(s) 300
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 63
HapII CCGG 4 cut(s) 173, 231, 512, 789
HgaI GACGC 2 cut(s) 296, 302
HhaI GCGC 1 cut(s) 62
Hin1II CATG 7 cut(s) 10, 30, 54, 412, 477, 827, 903
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 2 cut(s) 286, 436
HindII GTYRAC 2 cut(s) 286, 436
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 8 cut(s) 128, 326, 432, 506, 587, 855, 916, 983
HpaII CCGG 4 cut(s) 173, 231, 512, 789
HphI GGTGA 1 cut(s) 578
Hpy166II GTNNAC 4 cut(s) 286, 436, 794, 819
Hpy188I TCNGA 7 cut(s) 127, 431, 550, 586, 712, 838, 925
Hpy188III TCNNGA 1 cut(s) 626
Hpy8I GTNNAC 4 cut(s) 286, 436, 794, 819
HpyAV CCTTC 2 cut(s) 47, 239
HpyCH4III ACNGT 2 cut(s) 283, 1006
HpyCH4IV ACGT 2 cut(s) 561, 681
HpyCH4V TGCA 8 cut(s) 54, 269, 473, 695, 758, 851, 975, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 302
HpyF3I CTNAG 4 cut(s) 126, 142, 687, 711
HpySE526I ACGT 2 cut(s) 561, 681
Hsp92II CATG 7 cut(s) 10, 30, 54, 412, 477, 827, 903
HspAI GCGC 1 cut(s) 60
Kzo9I GATC 3 cut(s) 145, 185, 946
LmnI GCTCC 3 cut(s) 39, 404, 970
Lsp1109I GCAGC 1 cut(s) 1007
LweI GCATC 2 cut(s) 374, 769
MaeI CTAG 1 cut(s) 614
MaeII ACGT 2 cut(s) 561, 681
MaeIII GTNAC 5 cut(s) 277, 566, 665, 674, 877
MalI GATC 3 cut(s) 147, 187, 948
MboI GATC 3 cut(s) 145, 185, 946
MboII GAAGA 5 cut(s) 110, 333, 425, 490, 640
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 1 cut(s) 185
MluCI AATT 5 cut(s) 620, 753, 759, 832, 942
MlyI GAGTC 6 cut(s) 137, 335, 441, 515, 581, 925
MmeI TCCRAC 3 cut(s) 133, 163, 916
Mph1103I ATGCAT 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 3 cut(s) 93, 258, 885
MspI CCGG 4 cut(s) 173, 231, 512, 789
MspR9I CCNGG 3 cut(s) 231, 381, 772
MunI CAATTG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 2 cut(s) 381, 772
MwoI GCNNNNNNNGC 2 cut(s) 19, 302
NciI CCSGG 1 cut(s) 231
NcoI CCATGG 1 cut(s) 408
NdeII GATC 3 cut(s) 145, 185, 946
NlaIII CATG 7 cut(s) 10, 30, 54, 412, 477, 827, 903
NlaIV GGNNCC 3 cut(s) 187, 406, 423
NmuCI GTSAC 2 cut(s) 566, 674
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 2 cut(s) 827, 903
PciI ACATGT 1 cut(s) 899
PctI GAATGC 1 cut(s) 772
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 2 cut(s) 855, 983
PinAI ACCGGT 1 cut(s) 788
PkrI GCNGC 1 cut(s) 997
PleI GAGTC 6 cut(s) 136, 334, 440, 514, 581, 924
PpsI GAGTC 6 cut(s) 136, 334, 440, 514, 581, 924
Ppu21I YACGTR 1 cut(s) 682
PpuMI RGGWCCY 1 cut(s) 422
PscI ACATGT 1 cut(s) 899
PsiI TTATAA 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 379, 770
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 2 cut(s) 379, 770
PspN4I GGNNCC 3 cut(s) 187, 406, 423
PspPI GGNCC 2 cut(s) 377, 422
PspPPI RGGWCCY 1 cut(s) 422
PsrI GAACNNNNNNTAC 2 cut(s) 440, 472
PstI CTGCAG 1 cut(s) 997
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 3 cut(s) 684, 787, 793
RsaNI GTAC 3 cut(s) 683, 786, 792
SaqAI TTAA 3 cut(s) 93, 258, 885
SatI GCNGC 1 cut(s) 996
Sau3AI GATC 3 cut(s) 145, 185, 946
Sau96I GGNCC 2 cut(s) 377, 422
SchI GAGTC 6 cut(s) 137, 335, 441, 515, 581, 925
ScrFI CCNGG 3 cut(s) 231, 381, 772
SfaNI GCATC 2 cut(s) 374, 769
SfcI CTRYAG 1 cut(s) 993
SinI GGWCC 2 cut(s) 377, 422
SnaBI TACGTA 1 cut(s) 682
Sse9I AATT 5 cut(s) 620, 753, 759, 832, 942
SspI AATATT 1 cut(s) 866
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 3 cut(s) 229, 379, 770
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 2 cut(s) 283, 1006
TaiI ACGT 2 cut(s) 564, 684
TasI AATT 5 cut(s) 620, 753, 759, 832, 942
TfiI GAWTC 2 cut(s) 855, 983
Tru1I TTAA 3 cut(s) 93, 258, 885
Tru9I TTAA 3 cut(s) 93, 258, 885
TscAI CASTG 2 cut(s) 661, 853
TseFI GTSAC 2 cut(s) 566, 674
TseI GCWGC 1 cut(s) 995
Tsp45I GTSAC 2 cut(s) 566, 674
TspDTI ATGAA 2 cut(s) 23, 640
TspRI CASTG 2 cut(s) 661, 853
VpaK11BI GGWCC 2 cut(s) 377, 422
XapI RAATTY 2 cut(s) 620, 832
XceI RCATGY 2 cut(s) 827, 903
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.