RchiOBHm_Chr3g0466011

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000256
Physical Location & Seq
Forward (+)
12620565 .. 12623032
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43212

Sequence Viewer

Length: 2022 bp
ATGTTTGTCAAGCTTTCTGTTCGACTAGTACTACTAGTAAGTCTGGTAGCAGCAGAAGCCCAAGACCTCAATTTCATCTACAATGATGGTTTCGATGGCCGTTCTGGTCTTAATCTCAGTCTAGACGGCATAGCAGAGATCACACCGAAAGGTCTCTTAAAGCTTACAAACGACGCCAAACAGGAAAGTGGCCACGCCTTCTACCCTAACCCAGTAACCTTCAAGAACTCAGAGAACGACACCGCTTTCTCCTTCTCCACCATCTTTGTCTTTGCCATCCGATCAAAGTACACTACTCTCAGCGGCCATGGAATGGCCTTTGTCATTGCTCCGACGAGAAGCCTCCACGGAGCTCTGCCGAGCAATTACTTGGGCCTGTTGAACGAGTTTAACAATGGGAATTTCACCAATCATGTTTTTGCTGTCGAGCTTGACACGATCCAGACTCCGCAATTCAGTGACATCAATGACAACCATGTTGGAATCGACATCAATGGCTTGCACTCTGTCAGAGCTGCTCCAGCTGGTTTATTTGATGGTCAGTTCAAGAACCTGACTCTTAACAGTGGTAAAGAAATGCGAGTTTGGGTTGAATATGATGGTACCAAGAAGCAAATAGAAGTCACTATGGCTCCAATTGGTGTTGCAACTAAATCCCCAACTCCACTTTTGTCTTTGAAATATGACCTTTCCCCAATTCTCAACAAAACCATGTATGTTGGTTTTTCCTCTGCAACTGGTTCAGTGCCCACCTCCCATTATGTAGTGGGTTGGAGCTTTAGGATGAATGGCCAAGCTCAAGACCTTATAACTTCCAAACTTCCCAAGTTGCCCAGCATTGCAGGTAAAAAGAGGTCCATGCTTTTCACCTTTGGTGTGCCTCTGATTTCAGTGAGTTTGGTTTTGCTAGTGGTTTCTGGTGTGCTTTATGTCATAAGAAGGAAGAGGAAGTTTGCAGAAGTGCTTGAAGATTGGGAGCTAGAGTATGGTCCTCAGAGGTTTAAGTACAAAGAACTGTATATAGCCACCAAAGGGTTTAGGGAAAAGGAGCTTTTGGGAACTGGGGGATTTGGTAAAGTTTATAGAGGTTTATTACCCTCCTCTGAAGTTGAGATTGCAGTGAAGAGGGTATCACATGAATCAAGACAGGGGATGAAGGAATTTGTAGCAGAAATTGTTAGTATTGGCCGGCTTCGTCACCGGAATTTAGTACAACTGTTGGGATATTGCAGGCGAAAAGGGGAGCTGCTTTTGGTCTATGACTACATGCCTAATGGAAGCTTGGACAAGTACCTCTATGATCAACCTGAGGTGACCCTTAATTGGAGCCAGAGGTTTAAAGTCATCAAAGGTGTGGCTTCAGGGCTGTTCTATCTTCATGAAGAATGGGAACAGGTTGTGATTCATAGGGATGTGAAGGCCAGTAATGTGTTGGTAGATGGGGAATTGAATGGAAAGCTAGGAGATTTCGGGCTTGCAAGATTATACGACCATGGAACAGACCCTCAAACAACTCATATAGTTGGAACACTAGGGTACCTAGCCCCAGAGCACACAAGAACAGGTTGGGCCACCACGAGCACCGACGTGTTTGCTTTTGGGGCATTTTTGCTCGAAGTTGCTTGCGGAAAAAGGCCTATTGAGAGACAAGGTCCAGAAGCTGAGATTTTGCTTGATTGGGTATTTTCTTGTTGGAATAGAAGTAACATCCTTGAGGCAAGAGATCAAAGGTTTGGTACGGATTTTGTAGCCGAGGAATTGGAGTTGGTGTTGAAGCTTGGGCTTTTGTGCTCTCATTCGGAGCCAGCGGCAAGGCCAAGCATGCGACAAGTCGTTCAGTACTTGGCTGGTGATCTTGCTTTGCCGGAAGTGTCACTTCTCGGGCTTTCTTCTAGTGGCTTAGCGGTTGGACACCGTGAAGGTTTTGATGACTATGCTATGTCGTATCAGTCTTCCTTAGGCAAGGGGTCCTCGCATTCATCATATATTGCAGAGTCGGCATTACTTTCAGGTGGTCGTTGA

Protein Analysis

673

Amino Acids

74.47

Weight (kDa)

7.01

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 23 - 275 1.5e-82 Legume lectin domain
Lectin_L-type_dom PF18483 42 - 249 4.3e-07 Legume lectin beta-barrel domain
Pkinase PF00069 346 - 613 2.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 349 - 615 7.6e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07460 AT1G07460
fragaria_vesca FvH4_6g13200
malus_domestica MD12G1146600.v1.1 MD12G1147100.v1.1 MD12G1147300.v1.1 MD12G1147500.v1.1
prunus_persica Prupe.6G259500_v2.0.a1 Prupe.6G259800_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261500_v2.0.a1
pyrus_communis pycom04g12150 pycom12g14040 pycom12g14060 pycom12g14080 pycom12g14110
rosa_chinensis RchiOBHm_Chr3g0460311 RchiOBHm_Chr3g0465181 RchiOBHm_Chr3g0465701 RchiOBHm_Chr3g0465801 RchiOBHm_Chr3g0465861 RchiOBHm_Chr3g0465911 RchiOBHm_Chr3g0465931 RchiOBHm_Chr3g0465991 RchiOBHm_Chr3g0466011 RchiOBHm_Chr4g0404151 RchiOBHm_Chr6g0253601 RchiOBHm_Chr6g0259671 RchiOBHm_Chr7g0189621 RchiOBHm_Chr7g0189631 RchiOBHm_Chr7g0216481
rosa_laevigata RLG00000014514 RLG00000014515 RLG00000014999 RLG00000024609 RLG00000024614 RLG00000024616 RLG00000024620 RLG00000024624 RLG00000024627 RLG00000024650 RLG00000025025
rosa_multiflora Rmu_co8340115.1_g000001 Rmu_sc0000362.1_g000006 Rmu_sc0000449.1_g000013 Rmu_sc0000536.1_g000003 Rmu_sc0000536.1_g000006 Rmu_sc0002405.1_g000009 Rmu_sc0003391.1_g000002 Rmu_sc0003391.1_g000003 Rmu_sc0003391.1_g000013 Rmu_sc0003492.1_g000011 Rmu_sc0004305.1_g000015 Rmu_sc0004305.1_g000025 Rmu_sc0007705.1_g000012 Rmu_sc0007705.1_g000014 Rmu_sc0007705.1_g000015 Rmu_sc0007705.1_g000018 Rmu_sc0009386.1_g000006 Rmu_sc0009386.1_g000008 Rmu_sc0010030.1_g000001 Rmu_sc0010217.1_g000002 Rmu_sc0034485.1_g000001 Rmu_ssc0000454.1_g000025
rosa_roxburghii Rroxscaffold_3G00243190 Rroxscaffold_6G00413130 Rroxscaffold_6G00414850 Rroxscaffold_6G00414890 Rroxscaffold_6G00414910 Rroxscaffold_6G00414930 Rroxscaffold_6G00419650 Rroxscaffold_7G00206940
rosa_rugosa Rorug03G0041500 Rorug03G0078300 Rorug03G0078500 Rorug03G0078600 Rorug03G0078800 Rorug03G0078900 Rorug07G0160500
rosa_samantha Rh3BG103300 Rh3BG139800 Rh3BG143800 Rh3BG144300 Rh3BG145000 Rh3BG145500 Rh3BG145600 Rh3BG146000 Rh3CG104900 Rh3CG141500 Rh3CG145100 Rh3CG145800 Rh3CG146300 Rh3CG146500 Rh3CG146700 Rh3CG147000 Rh3CG149900 Rh3DG104600 Rh3DG141000 Rh3DG145100 Rh3DG145600 Rh3DG146100 Rh3DG146400 Rh3DG146600 Rh3DG146900 Rh4AG123200 Rh4BG116400 Rh6AG060500 Rh6BG101000 Rh7AG299800 Rh7BG291300 Rh7DG106700 Rh7DG106900
rosa_wichuraiana Rw3G008440 Rw3G011450 Rw3G011670 Rw3G011710 Rw3G011720 Rw3G011750 Rw3G011770 Rw3G011810 Rw6G005390 Rw7G009000 Rw7G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
Acc36I ACCTGC 1 cut(s) 833
Acc65I GGTACC 2 cut(s) 602, 1536
AccB1I GGYRCC 2 cut(s) 602, 1536
AccB7I CCANNNNNTGG 1 cut(s) 313
AciI CCGC 6 cut(s) 243, 303, 449, 1626, 1808, 1904
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 5 cut(s) 97, 190, 304, 790, 1186
AcsI RAATTY 3 cut(s) 400, 1160, 1204
AcuI CTGAAG 2 cut(s) 1125, 1344
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 9 cut(s) 30, 290, 604, 1007, 1212, 1292, 1538, 1738, 1841
AfiI CCNNNNNNNGG 3 cut(s) 313, 1032, 1323
AflIII ACRYGT 1 cut(s) 1587
AgsI TTSAA 8 cut(s) 223, 382, 547, 593, 679, 968, 1450, 1774
AhlI ACTAGT 2 cut(s) 25, 34
AjiI CACGTC 1 cut(s) 1588
AleI CACNNNNGTG 1 cut(s) 1586
Alw21I GWGCWC 4 cut(s) 355, 1554, 1583, 1793
Alw26I GTCTC 2 cut(s) 158, 1639
AlwI GGATC 1 cut(s) 433
AlwNI CAGNNNCTG 1 cut(s) 1661
Ama87I CYCGRG 1 cut(s) 1880
ApeKI GCWGC 3 cut(s) 50, 515, 1246
ApoI RAATTY 3 cut(s) 400, 1160, 1204
Asp718I GGTACC 2 cut(s) 602, 1536
AspS9I GGNCC 6 cut(s) 373, 855, 989, 1569, 1652, 1968
AsuHPI GGTGA 5 cut(s) 397, 859, 1190, 1324, 1862
AvaI CYCGRG 1 cut(s) 1880
AvaII GGWCC 4 cut(s) 855, 989, 1652, 1968
AxyI CCTNAGG 2 cut(s) 1308, 1957
BaeGI GKGCMC 1 cut(s) 750
BaeI ACNNNNGTAYC 2 cut(s) 1520, 1553
BalI TGGCCA 2 cut(s) 192, 792
BanI GGYRCC 2 cut(s) 602, 1536
BanII GRGCYC 1 cut(s) 355
BauI CACGAG 1 cut(s) 1576
BbsI GAAGAC 1 cut(s) 1944
Bbv12I GWGCWC 4 cut(s) 355, 1554, 1583, 1793
BbvI GCAGC 3 cut(s) 62, 502, 1233
BccI CCATC 7 cut(s) 80, 89, 269, 284, 530, 593, 1433
BceAI ACGGC 2 cut(s) 84, 142
BcgI CGANNNNNNTGC 2 cut(s) 1574, 1608
BclI TGATCA 1 cut(s) 1300
BcoDI GTCTC 2 cut(s) 158, 1639
BcuI ACTAGT 2 cut(s) 25, 34
BfaI CTAG 9 cut(s) 26, 35, 122, 908, 980, 1460, 1532, 1541, 1893
BfuAI ACCTGC 1 cut(s) 833
BisI GCNGC 5 cut(s) 51, 304, 516, 1247, 1809
BlpI GCTNAGC 1 cut(s) 1900
BlsI GCNGC 5 cut(s) 52, 305, 517, 1248, 1810
BmcAI AGTACT 2 cut(s) 30, 1841
Bme18I GGWCC 4 cut(s) 855, 989, 1652, 1968
BmeT110I CYCGRG 1 cut(s) 1880
BmgBI CACGTC 1 cut(s) 1588
BmgT120I GGNCC 6 cut(s) 373, 855, 989, 1569, 1652, 1968
BmiI GGNNCC 6 cut(s) 604, 633, 1328, 1538, 1803, 1969
BmrI ACTGGG 2 cut(s) 206, 1071
BmuI ACTGGG 2 cut(s) 206, 1071
BpiI GAAGAC 1 cut(s) 1944
BpmI CTGGAG 1 cut(s) 504
Bpu1102I GCTNAGC 1 cut(s) 1900
BpuEI CTTGAG 2 cut(s) 783, 1733
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 4 cut(s) 307, 346, 1492, 1752
BsaWI WCCGGW 1 cut(s) 1200
BsaXI ACNNNNNCTCC 6 cut(s) 616, 646, 968, 998, 1754, 1784
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 1032, 1323
Bse118I RCCGGY 1 cut(s) 1188
Bse1I ACTGG 4 cut(s) 212, 742, 1066, 1422
Bse21I CCTNAGG 2 cut(s) 1308, 1957
Bse3DI GCAATG 2 cut(s) 324, 837
BseDI CCNNGG 4 cut(s) 307, 346, 1492, 1752
BseGI GGATG 5 cut(s) 276, 789, 1158, 1417, 1707
BseLI CCNNNNNNNGG 3 cut(s) 313, 1032, 1323
BseMI GCAATG 2 cut(s) 324, 837
BseMII CTCAG 6 cut(s) 130, 243, 313, 1007, 1299, 1653
BseNI ACTGG 4 cut(s) 212, 742, 1066, 1422
BseRI GAGGAG 1 cut(s) 1090
BseSI GKGCMC 1 cut(s) 750
BseXI GCAGC 3 cut(s) 62, 502, 1233
BseYI CCCAGC 1 cut(s) 833
BshNI GGYRCC 2 cut(s) 602, 1536
BsiHKAI GWGCWC 4 cut(s) 355, 1554, 1583, 1793
BsiHKCI CYCGRG 1 cut(s) 1880
BsiSI CCGG 3 cut(s) 1189, 1201, 1865
BslI CCNNNNNNNGG 3 cut(s) 313, 1032, 1323
BsmAI GTCTC 2 cut(s) 158, 1639
BsmI GAATGC 1 cut(s) 1975
Bso31I GGTCTC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 1880
Bsp1286I GDGCHC 5 cut(s) 355, 750, 1554, 1583, 1793
Bsp143I GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
Bsp1720I GCTNAGC 1 cut(s) 1900
Bsp19I CCATGG 2 cut(s) 307, 1492
BspACI CCGC 6 cut(s) 243, 303, 449, 1626, 1808, 1904
BspCNI CTCAG 6 cut(s) 129, 242, 312, 1006, 1300, 1654
BspHI TCATGA 1 cut(s) 1378
BspLI GGNNCC 6 cut(s) 604, 633, 1328, 1538, 1803, 1969
BspMI ACCTGC 1 cut(s) 833
BspPI GGATC 1 cut(s) 433
BspT107I GGYRCC 2 cut(s) 602, 1536
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 324, 837
BsrFI RCCGGY 1 cut(s) 1188
BsrI ACTGG 4 cut(s) 212, 742, 1066, 1422
BssAI RCCGGY 1 cut(s) 1188
BssECI CCNNGG 4 cut(s) 307, 346, 1492, 1752
BssMI GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 1576
BssT1I CCWWGG 2 cut(s) 307, 1492
Bst2BI CACGAG 1 cut(s) 1576
Bst4CI ACNGT 4 cut(s) 566, 1017, 1218, 1916
Bst6I CTCTTC 2 cut(s) 938, 1118
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 7 cut(s) 500, 1190, 1232, 1476, 1624, 1806, 1823
BstDEI CTNAG 8 cut(s) 116, 229, 299, 993, 1308, 1662, 1900, 1957
BstDSI CCRYGG 3 cut(s) 307, 346, 1492
BstEII GGTNACC 1 cut(s) 1312
BstF5I GGATG 5 cut(s) 276, 789, 1158, 1417, 1707
BstKTI GATC 6 cut(s) 141, 284, 441, 1303, 1726, 1855
BstMAI GTCTC 2 cut(s) 158, 1639
BstMBI GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
BstMWI GCNNNNNNNGC 5 cut(s) 56, 521, 1601, 1822, 1997
BstNSI RCATGY 2 cut(s) 1270, 1825
BstPI GGTNACC 1 cut(s) 1312
BstSLI GKGCMC 1 cut(s) 750
BstV1I GCAGC 3 cut(s) 62, 502, 1233
BstV2I GAAGAC 1 cut(s) 1944
Bsu36I CCTNAGG 2 cut(s) 1308, 1957
BtgI CCRYGG 3 cut(s) 307, 346, 1492
BtrI CACGTC 1 cut(s) 1588
BtsCI GGATG 5 cut(s) 276, 789, 1158, 1417, 1707
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 5 cut(s) 463, 571, 750, 897, 1125
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 7 cut(s) 500, 1190, 1232, 1476, 1624, 1806, 1823
CaiI CAGNNNCTG 1 cut(s) 1661
CciI TCATGA 1 cut(s) 1378
Cfr10I RCCGGY 1 cut(s) 1188
Cfr13I GGNCC 6 cut(s) 373, 855, 989, 1569, 1652, 1968
CseI GACGC 1 cut(s) 182
Csp6I GTAC 9 cut(s) 29, 289, 603, 1006, 1211, 1291, 1537, 1737, 1840
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviQI GTAC 9 cut(s) 29, 289, 603, 1006, 1211, 1291, 1537, 1737, 1840
DdeI CTNAG 8 cut(s) 116, 229, 299, 993, 1308, 1662, 1900, 1957
DpnI GATC 6 cut(s) 140, 283, 440, 1302, 1725, 1854
DpnII GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
DraI TTTAAA 1 cut(s) 1339
EaeI YGGCCR 5 cut(s) 97, 190, 304, 790, 1186
Eam1104I CTCTTC 2 cut(s) 938, 1118
EarI CTCTTC 2 cut(s) 938, 1118
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 307, 1492
Eco147I AGGCCT 1 cut(s) 1636
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 4 cut(s) 855, 989, 1652, 1968
Eco53kI GAGCTC 1 cut(s) 353
Eco57I CTGAAG 2 cut(s) 1125, 1344
Eco81I CCTNAGG 2 cut(s) 1308, 1957
Eco88I CYCGRG 1 cut(s) 1880
Eco91I GGTNACC 1 cut(s) 1312
EcoICRI GAGCTC 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 1968
EcoO65I GGTNACC 1 cut(s) 1312
EcoT14I CCWWGG 2 cut(s) 307, 1492
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 2 cut(s) 307, 1492
FalI AAGNNNNNCTT 2 cut(s) 1860, 1892
FbaI TGATCA 1 cut(s) 1300
Fnu4HI GCNGC 5 cut(s) 51, 304, 516, 1247, 1809
FokI GGATG 5 cut(s) 263, 796, 1165, 1424, 1694
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 5 cut(s) 51, 304, 516, 1247, 1809
FspBI CTAG 9 cut(s) 26, 35, 122, 908, 980, 1460, 1532, 1541, 1893
GluI GCNGC 5 cut(s) 51, 304, 516, 1247, 1809
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 1 cut(s) 504
HapII CCGG 3 cut(s) 1189, 1201, 1865
HgaI GACGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 174
HindIII AAGCTT 4 cut(s) 11, 161, 1279, 1775
HinfI GANTC 6 cut(s) 445, 483, 556, 1139, 1402, 1994
HpaII CCGG 3 cut(s) 1189, 1201, 1865
HphI GGTGA 5 cut(s) 397, 859, 1190, 1324, 1862
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 8 cut(s) 232, 281, 333, 512, 885, 996, 1105, 1801
Hpy188III TCNNGA 8 cut(s) 122, 223, 442, 547, 800, 1143, 1379, 1655
Hpy8I GTNNAC 1 cut(s) 291
Hpy99I CGWCG 3 cut(s) 176, 337, 1589
HpyAV CCTTC 7 cut(s) 208, 229, 262, 933, 1150, 1411, 1913
HpyCH4III ACNGT 4 cut(s) 566, 1017, 1218, 1916
HpyCH4IV ACGT 1 cut(s) 1587
HpyCH4V TGCA 9 cut(s) 502, 647, 734, 842, 956, 1118, 1230, 1478, 1991
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 521, 1601, 1822, 1997
HpyF3I CTNAG 8 cut(s) 116, 229, 299, 993, 1308, 1662, 1900, 1957
HpySE526I ACGT 1 cut(s) 1587
Hsp92I GRCGYC 1 cut(s) 174
KpnI GGTACC 2 cut(s) 606, 1540
KroI GCCGGC 1 cut(s) 1188
KroNI GCCGGC 1 cut(s) 1190
Ksp22I TGATCA 1 cut(s) 1300
Kzo9I GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
Lsp1109I GCAGC 3 cut(s) 62, 502, 1233
MaeI CTAG 9 cut(s) 26, 35, 122, 908, 980, 1460, 1532, 1541, 1893
MaeII ACGT 1 cut(s) 1587
MaeIII GTNAC 7 cut(s) 214, 458, 622, 1196, 1312, 1703, 1872
MalI GATC 6 cut(s) 140, 283, 440, 1302, 1725, 1854
MboI GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
MboII GAAGA 7 cut(s) 955, 980, 1135, 1367, 1394, 1881, 1944
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 5 cut(s) 355, 750, 1554, 1583, 1793
MlsI TGGCCA 2 cut(s) 192, 792
MluNI TGGCCA 2 cut(s) 192, 792
MlyI GAGTC 3 cut(s) 439, 550, 2003
MmeI TCCRAC 6 cut(s) 356, 460, 752, 1504, 1673, 1888
Mox20I TGGCCA 2 cut(s) 192, 792
MroNI GCCGGC 1 cut(s) 1188
MscI TGGCCA 2 cut(s) 192, 792
MseI TTAA 7 cut(s) 111, 158, 390, 561, 1002, 1320, 1338
MslI CAYNNNNRTG 2 cut(s) 1410, 1586
Msp20I TGGCCA 2 cut(s) 192, 792
MspA1I CMGCKG 3 cut(s) 303, 524, 1808
MspI CCGG 3 cut(s) 1189, 1201, 1865
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 1975
MwoI GCNNNNNNNGC 5 cut(s) 56, 521, 1601, 1822, 1997
NaeI GCCGGC 1 cut(s) 1190
NcoI CCATGG 2 cut(s) 307, 1492
NdeII GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
NgoMIV GCCGGC 1 cut(s) 1188
NlaIV GGNNCC 6 cut(s) 604, 633, 1328, 1538, 1803, 1969
NmeAIII GCCGAG 2 cut(s) 384, 1777
NmuCI GTSAC 5 cut(s) 458, 622, 1196, 1312, 1872
NspI RCATGY 2 cut(s) 1270, 1825
OliI CACNNNNGTG 1 cut(s) 1586
PaeI GCATGC 1 cut(s) 1825
PagI TCATGA 1 cut(s) 1378
PceI AGGCCT 1 cut(s) 1636
PctI GAATGC 1 cut(s) 1975
PdiI GCCGGC 1 cut(s) 1190
PfeI GAWTC 3 cut(s) 483, 1139, 1402
PflFI GACNNNGTC 1 cut(s) 1650
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 5 cut(s) 52, 305, 517, 1248, 1810
PleI GAGTC 3 cut(s) 439, 550, 2002
PpsI GAGTC 3 cut(s) 439, 550, 2002
PpuMI RGGWCCY 1 cut(s) 1968
PsiI TTATAA 1 cut(s) 809
Psp124BI GAGCTC 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 1968
PspEI GGTNACC 1 cut(s) 1312
PspFI CCCAGC 1 cut(s) 833
PspN4I GGNNCC 6 cut(s) 604, 633, 1328, 1538, 1803, 1969
PspPI GGNCC 6 cut(s) 373, 855, 989, 1569, 1652, 1968
PspPPI RGGWCCY 1 cut(s) 1968
PstNI CAGNNNCTG 1 cut(s) 1661
PsyI GACNNNGTC 1 cut(s) 1650
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 9 cut(s) 30, 290, 604, 1007, 1212, 1292, 1538, 1738, 1841
RsaNI GTAC 9 cut(s) 29, 289, 603, 1006, 1211, 1291, 1537, 1737, 1840
RseI CAYNNNNRTG 2 cut(s) 1410, 1586
SacI GAGCTC 1 cut(s) 355
SaqAI TTAA 7 cut(s) 111, 158, 390, 561, 1002, 1320, 1338
SatI GCNGC 5 cut(s) 51, 304, 516, 1247, 1809
Sau3AI GATC 6 cut(s) 138, 281, 438, 1300, 1723, 1852
Sau96I GGNCC 6 cut(s) 373, 855, 989, 1569, 1652, 1968
ScaI AGTACT 2 cut(s) 30, 1841
SchI GAGTC 3 cut(s) 439, 550, 2003
SduI GDGCHC 5 cut(s) 355, 750, 1554, 1583, 1793
SinI GGWCC 4 cut(s) 855, 989, 1652, 1968
SmiMI CAYNNNNRTG 2 cut(s) 1410, 1586
SmlI CTYRAG 2 cut(s) 798, 1712
SmoI CTYRAG 2 cut(s) 798, 1712
SpeI ACTAGT 2 cut(s) 25, 34
SphI GCATGC 1 cut(s) 1825
SseBI AGGCCT 1 cut(s) 1636
SsiI CCGC 6 cut(s) 243, 303, 449, 1626, 1808, 1904
SspMI CTAG 9 cut(s) 26, 35, 122, 908, 980, 1460, 1532, 1541, 1893
SstI GAGCTC 1 cut(s) 355
StuI AGGCCT 1 cut(s) 1636
StyI CCWWGG 2 cut(s) 307, 1492
TaaI ACNGT 4 cut(s) 566, 1017, 1218, 1916
TaiI ACGT 1 cut(s) 1590
TaqI TCGA 5 cut(s) 22, 93, 426, 486, 1614
TatI WGTACW 5 cut(s) 28, 288, 1005, 1210, 1839
TauI GCSGC 2 cut(s) 306, 1811
TfiI GAWTC 3 cut(s) 483, 1139, 1402
Tru1I TTAA 7 cut(s) 111, 158, 390, 561, 1002, 1320, 1338
Tru9I TTAA 7 cut(s) 111, 158, 390, 561, 1002, 1320, 1338
TscAI CASTG 5 cut(s) 463, 571, 750, 897, 1125
TseFI GTSAC 5 cut(s) 458, 622, 1196, 1312, 1872
TseI GCWGC 3 cut(s) 50, 515, 1246
Tsp45I GTSAC 5 cut(s) 458, 622, 1196, 1312, 1872
TspDTI ATGAA 8 cut(s) 64, 800, 1152, 1169, 1367, 1394, 1395, 1968
TspGWI ACGGA 2 cut(s) 363, 1754
TspRI CASTG 5 cut(s) 463, 571, 750, 897, 1125
Tth111I GACNNNGTC 1 cut(s) 1650
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 4 cut(s) 855, 989, 1652, 1968
XapI RAATTY 3 cut(s) 400, 1160, 1204
XbaI TCTAGA 1 cut(s) 121
XceI RCATGY 2 cut(s) 1270, 1825
XcmI CCANNNNNNNNNTGG 1 cut(s) 1429
XspI CTAG 9 cut(s) 26, 35, 122, 908, 980, 1460, 1532, 1541, 1893
ZrmI AGTACT 2 cut(s) 30, 1841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.