Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0000256

Physical Location & Seq

Genomic Coordinates Reverse (-)

37106441 .. 37110297

Gene Structure 3857 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00414850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGTTTGTCAAGCTTTCTGTTCTACTAGTACTACTTGTAAGTCTGGTACCAGCAGAAGCTCAAGACCTCAATTTCATCTACAATGATGGTTTCAATGGCCGTTCTGGTCTTAATCTCAGTCTAGACGGCATAGCAGAGATCACACCGAAAGTACGCTACTCTCAGCGGCCATGGAATCGCCTTTGTCATCGCTCCGACGAGAGGCCTCCCCGGAGCTCTGGGAACCAGTACCTGAGCCTGTTCAACGTCTACAACAACGGGAATTTCACCAATCATGTTTTTGCTGTGGAGCTTGACACGCTCCAGAACCCGGAGTTCAATGACATCAATGAAAACCATGTTGGGATAGACATCAATGGCTTGCGCTCTGTCAAAGCTGCTCCAGCTGGTTATTTTGATAGTCAGTACTTCAAGAACCTGAGTCTCAGCAGTGGTAAAGAAATGAGAGTTTGGGTTGAATATGATGGTACCAAGAAGCAGATTGAAGTTTCTATGGCTCCAATTGCTGATGAATGGCCAAGCTCAAGACCTTATAGCTTCCAACTTCCAAAGTTGCCTAACGTTGCAGGTAAAAAGAGGTCCATGCTTTTCACCTTTGGTGTGCCTCTGATTTCAGTGAGTTTGGTATTGCTAGTGGTTTCTGGGGTACTTTATGTCATGAGAAGGAAGAGGAAGTTTGCAGAAGTGCTTGAAGATTGGGAGCTAGAGTATGGTCCTCAGAGGTTTAAGTACAAAGAACTGTATATAGCCACCAAAGGGTTTAGGGAAAAGGAGCTTTTGGGAACTGGGGGATTTGGTAAAGTTTATAGAGAAATTGTTAGTATTGGCCGGCTTCGTCACCGGAATTTAGTACAACTGGTGGGATATTGCAGGCGAAAAGGGGAGTTGCTTTTGGTCTATCACTACATGCCTAATGGAAGCTTGGACAAATACCTCTTTGATCAACCTGAGGTGACCCTTAATTGGAGCCATAGGTTTAAAGTTATCAAAGGTGTGGCTTCAGGGCTGTTCTATCTTCATGAAGAATGGGAACAGGTTGTGATTCATAGAGATGTGAAGGCCAGTAATGTGTTGCTAGATGGGGAATTCAATGGAAGGCTAGGAGACTTCGGGCTTGCAAGATTATACGACCATGGAACAGACCCTCAAACTACTCATATAGTTGGAACACTCGGGCCAATCAACACACAGGGTCCAGAAGATGTGATTTTGGTTGATTGTGTGTTTTCTTGTTGGAATCGAAGTAACATCCTTGAGGCAAGAGATCAGAGCTTTGGTACGGATTTTGTAGCCGAGGAAGTGGAGTTGGTGTTGAAGCTTGGGCTTTTGTGCTCTCATTCGGAGCCAGCGGCAAGGCCAAGCATGCGACAAGTCGTTCAGTACTTGGCTGGTGATGTTGCATTGCCGGAAGTGACTAGTGGCTTAGCGGTTGGACACCGTGAAGGTTTTGATGACTATGCTATGTCGTATCAGTCTTCCTTCGGCAGGCGCAGACAGGAACTTACTGGCCGCAAGCTCAGAGCGATATCGATTAGAATTAGAAGAGTGGTAAGAAAATACTACTACTACTCTGATTTAAAGACAGAGGAGAGGTGGTCTAATGGTCTCCCACAAGAAATCTTGCTATTGATTTTGCAAAGGCTATGCATATCGGATTACCTTGTGTTTCCAAAAGTATGTGTTTCTTGGCGAGCTGCTGTGGAGATGGGTATTGCTACCAAGAGTTGCCTTCCTGCTCCTCAACTTCCACGGCTTTTGCTCATCTCTCCTGCCCACCATTATATCAGTTCCTTCCACATTAAAGATCAAACTTGCTTTGTGAGTGATGGTGATCGGAAACTTGTTTCATTTGAATCATATGCCAGTGACATTTTATATTTCCTTAAGAAAGATCAGTCGTGGTTTTGGGGGATTTATTGTCTTGGTTCTACTGAGAGGTGGCTGATCATGGCTGAAGGTTATCCTATTGTCAACTTTTTTCCTCAACCCAGTATGAGTCATGCTCCCATCCCTACCAGACTGCACAGCTTCAAGAAAGTGGTAGCATCTTCAGTACCAACAAGGCTGCAGCATTGTCCTGTGGCTAGCATTACATCAGAAAGTCGTTTGGCTTTCTACAGGCCTACTGATACATCATGGACCCTCATCGAGCAGGCTGAGGCTGCTGCTGCCGAGGGGGAGGAGGAGGAGGCTCTCAAATTTTATGATATAGAAATACGTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0010033 GO:0014070 GO:0016020 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0044464 GO:0046677 GO:0048229 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

741

Amino Acids

84.47

Weight (kDa)

8.13

Isoelectric Point (pI)

49.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	68 - 180	1.5e-31	Legume lectin domain
Pkinase	PF00069	250 - 391	1.5e-22	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	267 - 378	7.3e-22	Protein tyrosine and serine/threonine kinase
F-box	PF00646	536 - 568	5.2e-06	F-box domain
Beta-prop_KIB1-4	PF03478	636 - 720	1e-06	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000256)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07460 AT1G07460
fragaria_vesca	FvH4_6g13200
malus_domestica	MD12G1146600.v1.1 MD12G1147100.v1.1 MD12G1147300.v1.1 MD12G1147500.v1.1
prunus_persica	Prupe.6G259500_v2.0.a1 Prupe.6G259800_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261500_v2.0.a1
pyrus_communis	pycom04g12150 pycom12g14040 pycom12g14060 pycom12g14080 pycom12g14110
rosa_chinensis	RchiOBHm_Chr3g0460311 RchiOBHm_Chr3g0465181 RchiOBHm_Chr3g0465701 RchiOBHm_Chr3g0465801 RchiOBHm_Chr3g0465861 RchiOBHm_Chr3g0465911 RchiOBHm_Chr3g0465931 RchiOBHm_Chr3g0465991 RchiOBHm_Chr3g0466011 RchiOBHm_Chr4g0404151 RchiOBHm_Chr6g0253601 RchiOBHm_Chr6g0259671 RchiOBHm_Chr7g0189621 RchiOBHm_Chr7g0189631 RchiOBHm_Chr7g0216481
rosa_laevigata	RLG00000014514 RLG00000014515 RLG00000014999 RLG00000024609 RLG00000024614 RLG00000024616 RLG00000024620 RLG00000024624 RLG00000024627 RLG00000024650 RLG00000025025
rosa_multiflora	Rmu_co8340115.1_g000001 Rmu_sc0000362.1_g000006 Rmu_sc0000449.1_g000013 Rmu_sc0000536.1_g000003 Rmu_sc0000536.1_g000006 Rmu_sc0002405.1_g000009 Rmu_sc0003391.1_g000002 Rmu_sc0003391.1_g000003 Rmu_sc0003391.1_g000013 Rmu_sc0003492.1_g000011 Rmu_sc0004305.1_g000015 Rmu_sc0004305.1_g000025 Rmu_sc0007705.1_g000012 Rmu_sc0007705.1_g000014 Rmu_sc0007705.1_g000015 Rmu_sc0007705.1_g000018 Rmu_sc0009386.1_g000006 Rmu_sc0009386.1_g000008 Rmu_sc0010030.1_g000001 Rmu_sc0010217.1_g000002 Rmu_sc0034485.1_g000001 Rmu_ssc0000454.1_g000025
rosa_roxburghii	Rroxscaffold_3G00243190 Rroxscaffold_6G00413130 Rroxscaffold_6G00414850 Rroxscaffold_6G00414890 Rroxscaffold_6G00414910 Rroxscaffold_6G00414930 Rroxscaffold_6G00419650 Rroxscaffold_7G00206940
rosa_rugosa	Rorug03G0041500 Rorug03G0078300 Rorug03G0078500 Rorug03G0078600 Rorug03G0078800 Rorug03G0078900 Rorug07G0160500
rosa_samantha	Rh3BG103300 Rh3BG139800 Rh3BG143800 Rh3BG144300 Rh3BG145000 Rh3BG145500 Rh3BG145600 Rh3BG146000 Rh3CG104900 Rh3CG141500 Rh3CG145100 Rh3CG145800 Rh3CG146300 Rh3CG146500 Rh3CG146700 Rh3CG147000 Rh3CG149900 Rh3DG104600 Rh3DG141000 Rh3DG145100 Rh3DG145600 Rh3DG146100 Rh3DG146400 Rh3DG146600 Rh3DG146900 Rh4AG123200 Rh4BG116400 Rh6AG060500 Rh6BG101000 Rh7AG299800 Rh7BG291300 Rh7DG106700 Rh7DG106900
rosa_wichuraiana	Rw3G008440 Rw3G011450 Rw3G011670 Rw3G011710 Rw3G011720 Rw3G011750 Rw3G011770 Rw3G011810 Rw6G005390 Rw7G009000 Rw7G025480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	557
Acc65I	GGTACC	2 cut(s)	46, 467
AccB1I	GGYRCC	2 cut(s)	46, 467
AccI	GTMKAC	1 cut(s)	249
AciI	CCGC	4 cut(s)	166, 1349, 1427, 1510
AclI	AACGTT	1 cut(s)	561
AcoI	YGGCCR	5 cut(s)	97, 167, 515, 826, 1507
AcsI	RAATTY	4 cut(s)	262, 844, 1085, 2198
AcuI	CTGAAG	3 cut(s)	984, 1974, 2034
AfiI	CCNNNNNNNGG	5 cut(s)	201, 310, 756, 963, 1485
AflII	CTTAAG	1 cut(s)	1883
AhlI	ACTAGT	2 cut(s)	25, 1415
AjuI	GAANNNNNNNTTGG	2 cut(s)	324, 356
Alw21I	GWGCWC	2 cut(s)	218, 1334
Alw26I	GTCTC	3 cut(s)	428, 1098, 1610
AlwNI	CAGNNNCTG	1 cut(s)	232
Ama87I	CYCGRG	1 cut(s)	1172
ApeKI	GCWGC	7 cut(s)	377, 1694, 2065, 2068, 2162, 2165, 2168
ApoI	RAATTY	4 cut(s)	262, 844, 1085, 2198
Asp718I	GGTACC	2 cut(s)	46, 467
AspLEI	GCGC	2 cut(s)	366, 1491
AspS9I	GGNCC	5 cut(s)	579, 713, 1175, 1193, 2139
AsuC2I	CCSGG	2 cut(s)	211, 311
AsuHPI	GGTGA	6 cut(s)	259, 583, 830, 964, 1403, 1841
AsuNHI	GCTAGC	1 cut(s)	2084
AvaI	CYCGRG	1 cut(s)	1172
AvaII	GGWCC	4 cut(s)	579, 713, 1193, 2139
AxyI	CCTNAGG	1 cut(s)	948
BalI	TGGCCA	1 cut(s)	517
BanI	GGYRCC	2 cut(s)	46, 467
BanII	GRGCYC	1 cut(s)	218
BbsI	GAAGAC	1 cut(s)	1467
Bbv12I	GWGCWC	2 cut(s)	218, 1334
BbvCI	CCTCAGC	1 cut(s)	2157
BbvI	GCAGC	7 cut(s)	364, 1681, 2052, 2080, 2149, 2152, 2155
BccI	CCATC	6 cut(s)	80, 458, 1073, 1699, 1820, 2015
BceAI	ACGGC	3 cut(s)	84, 142, 1766
BclI	TGATCA	2 cut(s)	940, 1944
BcnI	CCSGG	2 cut(s)	211, 311
BcoDI	GTCTC	3 cut(s)	428, 1098, 1610
BcuI	ACTAGT	2 cut(s)	25, 1415
BfaI	CTAG	8 cut(s)	26, 122, 632, 704, 1076, 1100, 1416, 2085
BfmI	CTRYAG	2 cut(s)	2066, 2116
BfrI	CTTAAG	1 cut(s)	1883
BfuAI	ACCTGC	1 cut(s)	557
BlpI	GCTNAGC	1 cut(s)	1423
BmcAI	AGTACT	3 cut(s)	30, 407, 1382
Bme1390I	CCNGG	2 cut(s)	211, 311
Bme18I	GGWCC	4 cut(s)	579, 713, 1193, 2139
BmeT110I	CYCGRG	1 cut(s)	1172
BmgT120I	GGNCC	5 cut(s)	579, 713, 1175, 1193, 2139
BmiI	GGNNCC	8 cut(s)	48, 224, 469, 498, 968, 1194, 1344, 2141
BmrFI	CCNGG	2 cut(s)	211, 311
BmrI	ACTGGG	2 cut(s)	795, 1983
BmsI	GCATC	1 cut(s)	2054
BmtI	GCTAGC	1 cut(s)	2088
BmuI	ACTGGG	2 cut(s)	795, 1983
BpiI	GAAGAC	1 cut(s)	1467
BplI	GAGNNNNNCTC	2 cut(s)	1987, 2019
BpmI	CTGGAG	2 cut(s)	287, 366
Bpu10I	CCTNAGC	2 cut(s)	233, 2157
Bpu1102I	GCTNAGC	1 cut(s)	1423
BpuEI	CTTGAG	3 cut(s)	45, 508, 1274
BpuMI	CCSGG	2 cut(s)	211, 311
Bsa29I	ATCGAT	1 cut(s)	1529
BsaAI	YACGTR	1 cut(s)	2219
BsaBI	GATNNNNATC	2 cut(s)	350, 1830
BsaI	GGTCTC	1 cut(s)	1610
BsaJI	CCNNGG	6 cut(s)	170, 209, 1132, 1293, 1748, 2172
BsaWI	WCCGGW	1 cut(s)	840
BsaXI	ACNNNNNCTCC	6 cut(s)	692, 722, 1295, 1325, 1580, 1610
Bsc4I	CCNNNNNNNGG	5 cut(s)	201, 310, 756, 963, 1485
Bse118I	RCCGGY	1 cut(s)	828
Bse1I	ACTGG	7 cut(s)	226, 790, 861, 1062, 1510, 1863, 1989
Bse21I	CCTNAGG	1 cut(s)	948
Bse3DI	GCAATG	1 cut(s)	1400
Bse8I	GATNNNNATC	2 cut(s)	350, 1830
BseCI	ATCGAT	1 cut(s)	1529
BseDI	CCNNGG	6 cut(s)	170, 209, 1132, 1293, 1748, 2172
BseGI	GGATG	2 cut(s)	1248, 2007
BseJI	GATNNNNATC	2 cut(s)	350, 1830
BseLI	CCNNNNNNNGG	5 cut(s)	201, 310, 756, 963, 1485
BseMI	GCAATG	1 cut(s)	1400
BseNI	ACTGG	7 cut(s)	226, 790, 861, 1062, 1510, 1863, 1989
BseRI	GAGGAG	5 cut(s)	1601, 1728, 2195, 2198, 2201
BseXI	GCAGC	7 cut(s)	364, 1681, 2052, 2080, 2149, 2152, 2155
BsgI	GTGCAG	1 cut(s)	2006
BshNI	GGYRCC	2 cut(s)	46, 467
BshVI	ATCGAT	1 cut(s)	1529
BsiHKAI	GWGCWC	2 cut(s)	218, 1334
BsiHKCI	CYCGRG	1 cut(s)	1172
BsiSI	CCGG	5 cut(s)	211, 311, 829, 841, 1406
BslI	CCNNNNNNNGG	5 cut(s)	201, 310, 756, 963, 1485
BsmAI	GTCTC	3 cut(s)	428, 1098, 1610
Bso31I	GGTCTC	1 cut(s)	1610
BsoBI	CYCGRG	1 cut(s)	1172
Bsp1286I	GDGCHC	2 cut(s)	218, 1334
Bsp143I	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
Bsp1720I	GCTNAGC	1 cut(s)	1423
Bsp19I	CCATGG	2 cut(s)	170, 1132
BspACI	CCGC	4 cut(s)	166, 1349, 1427, 1510
BspDI	ATCGAT	1 cut(s)	1529
BspHI	TCATGA	2 cut(s)	657, 1018
BspLI	GGNNCC	8 cut(s)	48, 224, 469, 498, 968, 1194, 1344, 2141
BspMAI	CTGCAG	1 cut(s)	2070
BspMI	ACCTGC	1 cut(s)	557
BspOI	GCTAGC	1 cut(s)	2088
BspT107I	GGYRCC	2 cut(s)	46, 467
BspTI	CTTAAG	1 cut(s)	1883
BspTNI	GGTCTC	1 cut(s)	1610
BsrDI	GCAATG	1 cut(s)	1400
BsrFI	RCCGGY	1 cut(s)	828
BsrI	ACTGG	7 cut(s)	226, 790, 861, 1062, 1510, 1863, 1989
BssAI	RCCGGY	1 cut(s)	828
BssECI	CCNNGG	6 cut(s)	170, 209, 1132, 1293, 1748, 2172
BssMI	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
BssT1I	CCWWGG	2 cut(s)	170, 1132
Bst4CI	ACNGT	2 cut(s)	741, 1439
Bst6I	CTCTTC	2 cut(s)	662, 1537
BstAFI	CTTAAG	1 cut(s)	1883
BstBAI	YACGTR	1 cut(s)	2219
BstDSI	CCRYGG	3 cut(s)	170, 1132, 1748
BstEII	GGTNACC	1 cut(s)	952
BstENI	CCTNNNNNAGG	1 cut(s)	1483
BstF5I	GGATG	2 cut(s)	1248, 2007
BstHHI	GCGC	2 cut(s)	366, 1491
BstKTI	GATC	7 cut(s)	141, 943, 1267, 1807, 1834, 1894, 1947
BstMAI	GTCTC	3 cut(s)	428, 1098, 1610
BstMBI	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
BstMWI	GCNNNNNNNGC	6 cut(s)	298, 383, 503, 1363, 2162, 2168
BstNSI	RCATGY	2 cut(s)	910, 1366
BstPI	GGTNACC	1 cut(s)	952
BstSCI	CCNGG	2 cut(s)	209, 309
BstSFI	CTRYAG	2 cut(s)	2066, 2116
BstSNI	TACGTA	1 cut(s)	2219
BstV1I	GCAGC	7 cut(s)	364, 1681, 2052, 2080, 2149, 2152, 2155
BstV2I	GAAGAC	1 cut(s)	1467
Bsu15I	ATCGAT	1 cut(s)	1529
Bsu36I	CCTNAGG	1 cut(s)	948
BsuTUI	ATCGAT	1 cut(s)	1529
BtgI	CCRYGG	3 cut(s)	170, 1132, 1748
BtgZI	GCGATG	1 cut(s)	173
BtsCI	GGATG	2 cut(s)	1248, 2007
BtsI	GCAGTG	1 cut(s)	436
BtsIMutI	CAGTG	3 cut(s)	436, 621, 1870
BveI	ACCTGC	1 cut(s)	557
CaiI	CAGNNNCTG	1 cut(s)	232
CciI	TCATGA	2 cut(s)	657, 1018
CfoI	GCGC	2 cut(s)	366, 1491
Cfr10I	RCCGGY	1 cut(s)	828
Cfr13I	GGNCC	5 cut(s)	579, 713, 1175, 1193, 2139
ClaI	ATCGAT	1 cut(s)	1529
CspCI	CAANNNNNGTGG	2 cut(s)	1737, 1772
DpnI	GATC	7 cut(s)	140, 942, 1266, 1806, 1833, 1893, 1946
DpnII	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
DraI	TTTAAA	2 cut(s)	979, 1578
EaeI	YGGCCR	5 cut(s)	97, 167, 515, 826, 1507
Eam1104I	CTCTTC	2 cut(s)	662, 1537
EarI	CTCTTC	2 cut(s)	662, 1537
Ecl136II	GAGCTC	1 cut(s)	216
Eco105I	TACGTA	1 cut(s)	2219
Eco130I	CCWWGG	2 cut(s)	170, 1132
Eco147I	AGGCCT	2 cut(s)	205, 2122
Eco24I	GRGCYC	1 cut(s)	218
Eco31I	GGTCTC	1 cut(s)	1610
Eco32I	GATATC	1 cut(s)	1527
Eco47I	GGWCC	4 cut(s)	579, 713, 1193, 2139
Eco53kI	GAGCTC	1 cut(s)	216
Eco57I	CTGAAG	3 cut(s)	984, 1974, 2034
Eco81I	CCTNAGG	1 cut(s)	948
Eco88I	CYCGRG	1 cut(s)	1172
Eco91I	GGTNACC	1 cut(s)	952
EcoICRI	GAGCTC	1 cut(s)	216
EcoNI	CCTNNNNNAGG	1 cut(s)	1483
EcoO65I	GGTNACC	1 cut(s)	952
EcoRI	GAATTC	1 cut(s)	1085
EcoRV	GATATC	1 cut(s)	1527
EcoT14I	CCWWGG	2 cut(s)	170, 1132
EcoT22I	ATGCAT	1 cut(s)	1649
EcoT38I	GRGCYC	1 cut(s)	218
ErhI	CCWWGG	2 cut(s)	170, 1132
FauNDI	CATATG	1 cut(s)	1858
FbaI	TGATCA	2 cut(s)	940, 1944
FblI	GTMKAC	1 cut(s)	249
FokI	GGATG	2 cut(s)	1235, 1994
FriOI	GRGCYC	1 cut(s)	218
FspBI	CTAG	8 cut(s)	26, 122, 632, 704, 1076, 1100, 1416, 2085
GlaI	GCGC	2 cut(s)	365, 1490
GsuI	CTGGAG	2 cut(s)	287, 366
HapII	CCGG	5 cut(s)	211, 311, 829, 841, 1406
HhaI	GCGC	2 cut(s)	366, 1491
Hin6I	GCGC	2 cut(s)	364, 1489
HinP1I	GCGC	2 cut(s)	364, 1489
HincII	GTYRAC	1 cut(s)	1972
HindII	GTYRAC	1 cut(s)	1972
HindIII	AAGCTT	3 cut(s)	11, 919, 1316
HinfI	GANTC	6 cut(s)	175, 421, 1042, 1237, 1853, 1996
HpaII	CCGG	5 cut(s)	211, 311, 829, 841, 1406
HphI	GGTGA	6 cut(s)	259, 583, 830, 964, 1403, 1841
Hpy166II	GTNNAC	2 cut(s)	250, 1972
Hpy188III	TCNNGA	9 cut(s)	62, 122, 304, 412, 525, 658, 1019, 1196, 2032
Hpy8I	GTNNAC	2 cut(s)	250, 1972
Hpy99I	CGWCG	1 cut(s)	200
HpyAV	CCTTC	8 cut(s)	657, 1051, 1089, 1436, 1489, 1739, 1801, 1949
HpyCH4III	ACNGT	2 cut(s)	741, 1439
HpyCH4IV	ACGT	3 cut(s)	246, 561, 2218
HpyCH4V	TGCA	9 cut(s)	566, 680, 870, 1118, 1400, 1636, 1647, 2023, 2068
HpyF10VI	GCNNNNNNNGC	6 cut(s)	298, 383, 503, 1363, 2162, 2168
HpySE526I	ACGT	3 cut(s)	246, 561, 2218
HspAI	GCGC	2 cut(s)	364, 1489
KpnI	GGTACC	2 cut(s)	50, 471
KroI	GCCGGC	1 cut(s)	828
KroNI	GCCGGC	1 cut(s)	830
Ksp22I	TGATCA	2 cut(s)	940, 1944
Kzo9I	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
Lsp1109I	GCAGC	7 cut(s)	364, 1681, 2052, 2080, 2149, 2152, 2155
LweI	GCATC	1 cut(s)	2054
MaeI	CTAG	8 cut(s)	26, 122, 632, 704, 1076, 1100, 1416, 2085
MaeII	ACGT	3 cut(s)	246, 561, 2218
MaeIII	GTNAC	5 cut(s)	836, 952, 1244, 1411, 1865
MalI	GATC	7 cut(s)	140, 942, 1266, 1806, 1833, 1893, 1946
MboI	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
MboII	GAAGA	8 cut(s)	679, 704, 1007, 1034, 1211, 1467, 1554, 2040
MfeI	CAATTG	1 cut(s)	501
MhlI	GDGCHC	2 cut(s)	218, 1334
MlsI	TGGCCA	1 cut(s)	517
MluCI	AATT	9 cut(s)	70, 262, 501, 813, 844, 961, 1085, 1536, 2198
MluNI	TGGCCA	1 cut(s)	517
MlyI	GAGTC	2 cut(s)	430, 2005
MmeI	TCCRAC	5 cut(s)	219, 565, 1144, 1214, 1411
Mox20I	TGGCCA	1 cut(s)	517
Mph1103I	ATGCAT	1 cut(s)	1649
MroNI	GCCGGC	1 cut(s)	828
MscI	TGGCCA	1 cut(s)	517
MseI	TTAA	7 cut(s)	111, 726, 960, 978, 1577, 1800, 1884
MslI	CAYNNNNRTG	1 cut(s)	1050
Msp20I	TGGCCA	1 cut(s)	517
MspA1I	CMGCKG	3 cut(s)	166, 386, 1349
MspCI	CTTAAG	1 cut(s)	1883
MspI	CCGG	5 cut(s)	211, 311, 829, 841, 1406
MspR9I	CCNGG	2 cut(s)	211, 311
MunI	CAATTG	1 cut(s)	501
MwoI	GCNNNNNNNGC	6 cut(s)	298, 383, 503, 1363, 2162, 2168
NaeI	GCCGGC	1 cut(s)	830
NciI	CCSGG	2 cut(s)	211, 311
NcoI	CCATGG	2 cut(s)	170, 1132
NdeI	CATATG	1 cut(s)	1858
NdeII	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
NgoMIV	GCCGGC	1 cut(s)	828
NheI	GCTAGC	1 cut(s)	2084
NlaIV	GGNNCC	8 cut(s)	48, 224, 469, 498, 968, 1194, 1344, 2141
NmeAIII	GCCGAG	2 cut(s)	1318, 2197
NmuCI	GTSAC	4 cut(s)	836, 952, 1411, 1865
NsiI	ATGCAT	1 cut(s)	1649
NspI	RCATGY	2 cut(s)	910, 1366
PaeI	GCATGC	1 cut(s)	1366
PagI	TCATGA	2 cut(s)	657, 1018
PceI	AGGCCT	2 cut(s)	205, 2122
PdiI	GCCGGC	1 cut(s)	830
PfeI	GAWTC	4 cut(s)	175, 1042, 1237, 1853
PleI	GAGTC	2 cut(s)	429, 2004
PpsI	GAGTC	2 cut(s)	429, 2004
Ppu21I	YACGTR	1 cut(s)	2219
Psp124BI	GAGCTC	1 cut(s)	218
Psp1406I	AACGTT	1 cut(s)	561
PspEI	GGTNACC	1 cut(s)	952
PspN4I	GGNNCC	8 cut(s)	48, 224, 469, 498, 968, 1194, 1344, 2141
PspPI	GGNCC	5 cut(s)	579, 713, 1175, 1193, 2139
PstI	CTGCAG	1 cut(s)	2070
PstNI	CAGNNNCTG	1 cut(s)	232
PvuII	CAGCTG	1 cut(s)	386
RseI	CAYNNNNRTG	1 cut(s)	1050
SacI	GAGCTC	1 cut(s)	218
SaqAI	TTAA	7 cut(s)	111, 726, 960, 978, 1577, 1800, 1884
Sau3AI	GATC	7 cut(s)	138, 940, 1264, 1804, 1831, 1891, 1944
Sau96I	GGNCC	5 cut(s)	579, 713, 1175, 1193, 2139
ScaI	AGTACT	3 cut(s)	30, 407, 1382
SchI	GAGTC	2 cut(s)	430, 2005
ScrFI	CCNGG	2 cut(s)	211, 311
SduI	GDGCHC	2 cut(s)	218, 1334
SfaNI	GCATC	1 cut(s)	2054
SfcI	CTRYAG	2 cut(s)	2066, 2116
SinI	GGWCC	4 cut(s)	579, 713, 1193, 2139
SmiMI	CAYNNNNRTG	1 cut(s)	1050
SmlI	CTYRAG	4 cut(s)	60, 523, 1253, 1883
SmoI	CTYRAG	4 cut(s)	60, 523, 1253, 1883
SnaBI	TACGTA	1 cut(s)	2219
SpeI	ACTAGT	2 cut(s)	25, 1415
SphI	GCATGC	1 cut(s)	1366
Sse9I	AATT	9 cut(s)	70, 262, 501, 813, 844, 961, 1085, 1536, 2198
SseBI	AGGCCT	2 cut(s)	205, 2122
SsiI	CCGC	4 cut(s)	166, 1349, 1427, 1510
SspMI	CTAG	8 cut(s)	26, 122, 632, 704, 1076, 1100, 1416, 2085
SstI	GAGCTC	1 cut(s)	218
StuI	AGGCCT	2 cut(s)	205, 2122
StyD4I	CCNGG	2 cut(s)	209, 309
StyI	CCWWGG	2 cut(s)	170, 1132
TaaI	ACNGT	2 cut(s)	741, 1439
TaiI	ACGT	3 cut(s)	249, 564, 2221
TaqI	TCGA	3 cut(s)	1240, 1529, 2148
TasI	AATT	9 cut(s)	70, 262, 501, 813, 844, 961, 1085, 1536, 2198
TatI	WGTACW	5 cut(s)	28, 405, 729, 850, 1380
TauI	GCSGC	3 cut(s)	169, 1352, 1512
TfiI	GAWTC	4 cut(s)	175, 1042, 1237, 1853
Tru1I	TTAA	7 cut(s)	111, 726, 960, 978, 1577, 1800, 1884
Tru9I	TTAA	7 cut(s)	111, 726, 960, 978, 1577, 1800, 1884
TscAI	CASTG	3 cut(s)	436, 621, 1870
TseFI	GTSAC	4 cut(s)	836, 952, 1411, 1865
TseI	GCWGC	7 cut(s)	377, 1694, 2065, 2068, 2162, 2165, 2168
Tsp45I	GTSAC	4 cut(s)	836, 952, 1411, 1865
TspDTI	ATGAA	7 cut(s)	64, 345, 525, 1007, 1034, 1035, 1836
TspGWI	ACGGA	1 cut(s)	1295
TspRI	CASTG	3 cut(s)	436, 621, 1870
Vha464I	CTTAAG	1 cut(s)	1883
VpaK11BI	GGWCC	4 cut(s)	579, 713, 1193, 2139
XagI	CCTNNNNNAGG	1 cut(s)	1483
XapI	RAATTY	4 cut(s)	262, 844, 1085, 2198
XbaI	TCTAGA	1 cut(s)	121
XceI	RCATGY	2 cut(s)	910, 1366
XmiI	GTMKAC	1 cut(s)	249
XspI	CTAG	8 cut(s)	26, 122, 632, 704, 1076, 1100, 1416, 2085
ZrmI	AGTACT	3 cut(s)	30, 407, 1382
Zsp2I	ATGCAT	1 cut(s)	1649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.