Rw3G011770

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000256
Physical Location & Seq
Forward (+)
11599589 .. 11602047
2459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011770.1

Sequence Viewer

Length: 2037 bp
ATGTTTGTCAAGGTTTCTGTTCTCCTAGTACTACTAGTACTTCTGGCACCAGCAGAAGCCCAAGACCTCAATTTCATCTACAATGATGGTTTCACTGGCCGTTCTGGTCTTAATCTCAGTTTAGACGGCATAGCAGAGATCACACCAACAGGTCTCTTGAAGCTTACAAACGACACCAAACTGAAAAGTGGTCATGCCTTCTACCCTAACCCAGTAACCTTCAAGAACTCAGAGAACGACACCGCTTTCTCCTTCTCCACCAACTTTGTTTTTGCAATCCGATCAGAGTACACTACTCTCAGCGCCCATGGAATCGCCTTTGTCATCGCTCCGACGAGAGGCCTCCCCGGAGCTCTGCCGAGCCAGTACTTGAGCCTGTTCAACGGGTCCAACAATGGGAATTTCTCCAATCATGTTTTTGCTGTGGAGCTTGACACGATCCAGAACACGGAATTCAGTGACATCAATGAAAACCATGTTGGGATCGACATCAATGGCTTGCACTCTGTCAAAGCTGCTGCAGCTGGTTATTTTGATGGTCAGTTCAAGAACCTGAGTCTTATCAGTGGTAAAGAAATGAGAGTTTGGGTTGAATATGATGGTACCAAGAAGCAAATTGAAGTTACTATTGCTCCAATTGCTGTTGCAACGAAACCCACAACTCCACTTTTGTCTTTGAAATATGACCTTTCCCCAATTCTCAACAAAACCATGTATGTTGGCTTTTCTTCTTCAACTGGTTCATTATTCACATCCCATTATGTAGTGGGTTGGAGCTTTAGGATGAATGGCCAGCTCAAGACCATAGCTTCCAAAAGGCAGCATGCAAGCTTCCACCGCGGTGGCCATAGCATTGGTAAAAAGAGGTCCATGCTTTTCACCTTTGGTGTGCCTCTGATTTCAGTGAATTTGTTTTTGCTGGTGGTTTCTGGGGTGCTTTATGTCATAAGAAGGAAGAGGAAGTTTGCAGAAGTGCTTGAAGATTGGGAGCTAGAGTATGGTCCTCAGAGGTTTAAGTACAAAGAACTGTATATAGCCACCAAAGGGTTTCGGGAAAAGGGGCTTTTGGGAACTGGGGGATTTGGTAAAGTTTATAGAGGTTTATTACCCTCCTCTAAAATTGAGATTGCAGTGAAGAGGGTATCACATGAATCAAGACAGGGGATGAAGGAATTTGTAGCAGAAATTGCTAGTATTGGCAGGCTTCGTCACCGGAATTTAGTACAACTGTTAGGATATTGTAGGCGAAAAGGGGAGCTGCTTTTGGTCTATGACTACATGCCTAATGGAAGCTTGGACAAATACCTCTATGATCAACCTCAGGTGACCCTTAATTGGAGCCAGAGGTTTAAAGTCATCAAAGGTGTGGCTTCTGGACTGTTATATCTTCATGAAGAATGGGAACAGGTTGTGATTCATAGAGATGTGAAGGCCAGTAATGTGTTGCTAGATGGGGAAATGAATGGAAGGCTAGGAGATTTCGGGCTTGCAAGATTATACGACCATGGAACAGACCCTCAAACTACTCATATAGTTGGAACACTTGGGTACCTAGCCCCAGAGCACACAAGAACAGGTCGGGCCACCACGAGCACCGACGTGTTTGCTTTTGGGGCATTTTTGCTCGAAGTTGCTTGTGGAAAAAGGCCAATTAAGACACAGGGTCCAGAAGATGTGATTTTGGTTGATTGGGTGTTTTCTTGTTGGAATAGGAGTAACATCCTTGAGGCAAGAGATCAGAGCATTGGTATGGATTTTGTAGCCGAGGAAGTGGAGTTGGTGTTGAAGCTTGGGCTTTTGTGCTCTCATTCGGAGCCAGCGGCAAGGCCTAGCATGCGACAAGTCGTTCAGTACTTGGCCAGTGATGCTGCTTTGCCGGAAGTGTCACTTCTCGGGCTTTCTTCTAGTGGCTTAACAGGTGGACACCGTGATGGTTTTGATGACTATACTTTGTCGTATCAGTCTTCCTTAGGCAATAAGTTCTCGCATCATTCATCATATGTTGCAGAGTCGGCACTACTTTCAGGTGGTCGTTGA

Protein Analysis

678

Amino Acids

74.96

Weight (kDa)

8.78

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 23 - 271 7.4e-82 Legume lectin domain
Lectin_L-type_dom PF18483 41 - 253 6.1e-10 Legume lectin beta-barrel domain
Pkinase PF00069 351 - 617 1.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 355 - 619 1.6e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07460 AT1G07460
fragaria_vesca FvH4_6g13200
malus_domestica MD12G1146600.v1.1 MD12G1147100.v1.1 MD12G1147300.v1.1 MD12G1147500.v1.1
prunus_persica Prupe.6G259500_v2.0.a1 Prupe.6G259800_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261500_v2.0.a1
pyrus_communis pycom04g12150 pycom12g14040 pycom12g14060 pycom12g14080 pycom12g14110
rosa_chinensis RchiOBHm_Chr3g0460311 RchiOBHm_Chr3g0465181 RchiOBHm_Chr3g0465701 RchiOBHm_Chr3g0465801 RchiOBHm_Chr3g0465861 RchiOBHm_Chr3g0465911 RchiOBHm_Chr3g0465931 RchiOBHm_Chr3g0465991 RchiOBHm_Chr3g0466011 RchiOBHm_Chr4g0404151 RchiOBHm_Chr6g0253601 RchiOBHm_Chr6g0259671 RchiOBHm_Chr7g0189621 RchiOBHm_Chr7g0189631 RchiOBHm_Chr7g0216481
rosa_laevigata RLG00000014514 RLG00000014515 RLG00000014999 RLG00000024609 RLG00000024614 RLG00000024616 RLG00000024620 RLG00000024624 RLG00000024627 RLG00000024650 RLG00000025025
rosa_multiflora Rmu_co8340115.1_g000001 Rmu_sc0000362.1_g000006 Rmu_sc0000449.1_g000013 Rmu_sc0000536.1_g000003 Rmu_sc0000536.1_g000006 Rmu_sc0002405.1_g000009 Rmu_sc0003391.1_g000002 Rmu_sc0003391.1_g000003 Rmu_sc0003391.1_g000013 Rmu_sc0003492.1_g000011 Rmu_sc0004305.1_g000015 Rmu_sc0004305.1_g000025 Rmu_sc0007705.1_g000012 Rmu_sc0007705.1_g000014 Rmu_sc0007705.1_g000015 Rmu_sc0007705.1_g000018 Rmu_sc0009386.1_g000006 Rmu_sc0009386.1_g000008 Rmu_sc0010030.1_g000001 Rmu_sc0010217.1_g000002 Rmu_sc0034485.1_g000001 Rmu_ssc0000454.1_g000025
rosa_roxburghii Rroxscaffold_3G00243190 Rroxscaffold_6G00413130 Rroxscaffold_6G00414850 Rroxscaffold_6G00414890 Rroxscaffold_6G00414910 Rroxscaffold_6G00414930 Rroxscaffold_6G00419650 Rroxscaffold_7G00206940
rosa_rugosa Rorug03G0041500 Rorug03G0078300 Rorug03G0078500 Rorug03G0078600 Rorug03G0078800 Rorug03G0078900 Rorug07G0160500
rosa_samantha Rh3BG103300 Rh3BG139800 Rh3BG143800 Rh3BG144300 Rh3BG145000 Rh3BG145500 Rh3BG145600 Rh3BG146000 Rh3CG104900 Rh3CG141500 Rh3CG145100 Rh3CG145800 Rh3CG146300 Rh3CG146500 Rh3CG146700 Rh3CG147000 Rh3CG149900 Rh3DG104600 Rh3DG141000 Rh3DG145100 Rh3DG145600 Rh3DG146100 Rh3DG146400 Rh3DG146600 Rh3DG146900 Rh4AG123200 Rh4BG116400 Rh6AG060500 Rh6BG101000 Rh7AG299800 Rh7BG291300 Rh7DG106700 Rh7DG106900
rosa_wichuraiana Rw3G008440 Rw3G011450 Rw3G011670 Rw3G011710 Rw3G011720 Rw3G011750 Rw3G011770 Rw3G011810 Rw6G005390 Rw7G009000 Rw7G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 602, 1548
AccB1I GGYRCC 3 cut(s) 46, 602, 1548
AccII CGCG 1 cut(s) 840
AciI CCGC 4 cut(s) 243, 838, 840, 1820
AclWI GGATC 2 cut(s) 433, 491
AcoI YGGCCR 4 cut(s) 97, 790, 844, 1857
AcsI RAATTY 5 cut(s) 400, 452, 907, 1172, 1216
AfaI GTAC 9 cut(s) 30, 39, 290, 368, 604, 1019, 1224, 1550, 1853
AfiI CCNNNNNNNGG 5 cut(s) 338, 396, 448, 1044, 1335
AflIII ACRYGT 1 cut(s) 1599
AhdI GACNNNNNGTC 1 cut(s) 1662
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 1600
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AleI CACNNNNGTG 2 cut(s) 840, 1598
Alw21I GWGCWC 4 cut(s) 355, 1566, 1595, 1805
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 2 cut(s) 433, 491
Ama87I CYCGRG 1 cut(s) 1892
AoxI GGCC 9 cut(s) 97, 340, 790, 844, 1431, 1581, 1646, 1826, 1857
ApeKI GCWGC 6 cut(s) 515, 518, 521, 820, 1258, 1868
ApoI RAATTY 5 cut(s) 400, 452, 907, 1172, 1216
Asp718I GGTACC 2 cut(s) 602, 1548
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 5 cut(s) 387, 867, 1001, 1581, 1664
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 3 cut(s) 871, 1202, 1336
AvaI CYCGRG 1 cut(s) 1892
AvaII GGWCC 4 cut(s) 387, 867, 1001, 1664
AxyI CCTNAGG 2 cut(s) 1320, 1969
BaeI ACNNNNGTAYC 2 cut(s) 1532, 1565
BalI TGGCCA 3 cut(s) 792, 846, 1859
BanI GGYRCC 3 cut(s) 46, 602, 1548
BanII GRGCYC 1 cut(s) 355
BauI CACGAG 1 cut(s) 1588
BbsI GAAGAC 1 cut(s) 1956
Bbv12I GWGCWC 4 cut(s) 355, 1566, 1595, 1805
BbvI GCAGC 6 cut(s) 502, 505, 533, 832, 1245, 1855
BccI CCATC 5 cut(s) 80, 530, 593, 1445, 1925
BceAI ACGGC 2 cut(s) 84, 142
BcgI CGANNNNNNTGC 2 cut(s) 1586, 1620
BclI TGATCA 1 cut(s) 1312
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 158
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 9 cut(s) 26, 35, 992, 1191, 1448, 1472, 1553, 1830, 1905
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 1 cut(s) 306
BisI GCNGC 7 cut(s) 516, 519, 522, 821, 1259, 1821, 1869
BlsI GCNGC 7 cut(s) 517, 520, 523, 822, 1260, 1822, 1870
BmcAI AGTACT 4 cut(s) 30, 39, 368, 1853
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 4 cut(s) 387, 867, 1001, 1664
BmeRI GACNNNNNGTC 1 cut(s) 1662
BmeT110I CYCGRG 1 cut(s) 1892
BmgBI CACGTC 1 cut(s) 1600
BmgT120I GGNCC 5 cut(s) 387, 867, 1001, 1581, 1664
BmiI GGNNCC 7 cut(s) 48, 388, 604, 1340, 1550, 1665, 1815
BmrFI CCNGG 1 cut(s) 348
BmrI ACTGGG 2 cut(s) 206, 1083
BmsI GCATC 2 cut(s) 1855, 1996
BmuI ACTGGG 2 cut(s) 206, 1083
BpiI GAAGAC 1 cut(s) 1956
BpuEI CTTGAG 3 cut(s) 391, 782, 1745
BpuMI CCSGG 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 5 cut(s) 307, 346, 838, 1504, 1764
BsaWI WCCGGW 1 cut(s) 1212
BsaXI ACNNNNNCTCC 6 cut(s) 616, 646, 980, 1010, 1766, 1796
Bsc4I CCNNNNNNNGG 5 cut(s) 338, 396, 448, 1044, 1335
Bse1I ACTGG 7 cut(s) 100, 212, 364, 742, 1078, 1434, 1860
Bse21I CCTNAGG 2 cut(s) 1320, 1969
Bse8I GATNNNNATC 1 cut(s) 488
BseDI CCNNGG 5 cut(s) 307, 346, 838, 1504, 1764
BseGI GGATG 4 cut(s) 752, 789, 1170, 1719
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 5 cut(s) 338, 396, 448, 1044, 1335
BseMII CTCAG 6 cut(s) 130, 243, 313, 545, 1019, 1334
BseNI ACTGG 7 cut(s) 100, 212, 364, 742, 1078, 1434, 1860
BseRI GAGGAG 1 cut(s) 1102
BseXI GCAGC 6 cut(s) 502, 505, 533, 832, 1245, 1855
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 9 cut(s) 99, 342, 792, 846, 1433, 1583, 1648, 1828, 1859
BshNI GGYRCC 3 cut(s) 46, 602, 1548
BsiHKAI GWGCWC 4 cut(s) 355, 1566, 1595, 1805
BsiHKCI CYCGRG 1 cut(s) 1892
BsiSI CCGG 3 cut(s) 348, 1213, 1877
BslI CCNNNNNNNGG 5 cut(s) 338, 396, 448, 1044, 1335
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 9 cut(s) 99, 342, 792, 846, 1433, 1583, 1648, 1828, 1859
Bso31I GGTCTC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 1892
Bsp1286I GDGCHC 4 cut(s) 355, 1566, 1595, 1805
Bsp143I GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
Bsp19I CCATGG 2 cut(s) 307, 1504
BspACI CCGC 4 cut(s) 243, 838, 840, 1820
BspANI GGCC 9 cut(s) 99, 342, 792, 846, 1433, 1583, 1648, 1828, 1859
BspCNI CTCAG 6 cut(s) 129, 242, 312, 546, 1018, 1333
BspFNI CGCG 1 cut(s) 840
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 7 cut(s) 48, 388, 604, 1340, 1550, 1665, 1815
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 2 cut(s) 433, 491
BspT107I GGYRCC 3 cut(s) 46, 602, 1548
BspTNI GGTCTC 1 cut(s) 158
BsrI ACTGG 7 cut(s) 100, 212, 364, 742, 1078, 1434, 1860
BssECI CCNNGG 5 cut(s) 307, 346, 838, 1504, 1764
BssMI GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
BssSI CACGAG 1 cut(s) 1588
BssT1I CCWWGG 2 cut(s) 307, 1504
Bst2BI CACGAG 1 cut(s) 1588
Bst4CI ACNGT 4 cut(s) 1029, 1230, 1380, 1928
Bst6I CTCTTC 2 cut(s) 950, 1130
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstC8I GCNNGC 8 cut(s) 500, 794, 825, 829, 1202, 1488, 1818, 1835
BstDEI CTNAG 7 cut(s) 116, 229, 299, 554, 1005, 1320, 1969
BstDSI CCRYGG 3 cut(s) 307, 838, 1504
BstEII GGTNACC 1 cut(s) 1324
BstF5I GGATG 4 cut(s) 752, 789, 1170, 1719
BstFNI CGCG 1 cut(s) 840
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 6 cut(s) 141, 284, 441, 486, 1315, 1738
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
BstMWI GCNNNNNNNGC 8 cut(s) 521, 638, 837, 1187, 1613, 1834, 1865, 2012
BstNSI RCATGY 3 cut(s) 827, 1282, 1837
BstPI GGTNACC 1 cut(s) 1324
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 6 cut(s) 502, 505, 533, 832, 1245, 1855
BstV2I GAAGAC 1 cut(s) 1956
BstXI CCANNNNNNTGG 2 cut(s) 842, 854
Bsu36I CCTNAGG 2 cut(s) 1320, 1969
BsuRI GGCC 9 cut(s) 99, 342, 792, 846, 1433, 1583, 1648, 1828, 1859
BtgI CCRYGG 3 cut(s) 307, 838, 1504
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 1600
BtsCI GGATG 4 cut(s) 752, 789, 1170, 1719
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 6 cut(s) 93, 463, 571, 909, 1137, 1867
Cac8I GCNNGC 8 cut(s) 500, 794, 825, 829, 1202, 1488, 1818, 1835
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 5 cut(s) 387, 867, 1001, 1581, 1664
Cfr42I CCGCGG 1 cut(s) 841
Csp6I GTAC 9 cut(s) 29, 38, 289, 367, 603, 1018, 1223, 1549, 1852
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviQI GTAC 9 cut(s) 29, 38, 289, 367, 603, 1018, 1223, 1549, 1852
DdeI CTNAG 7 cut(s) 116, 229, 299, 554, 1005, 1320, 1969
DpnI GATC 6 cut(s) 140, 283, 440, 485, 1314, 1737
DpnII GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
DraI TTTAAA 1 cut(s) 1351
DriI GACNNNNNGTC 1 cut(s) 1662
EaeI YGGCCR 4 cut(s) 97, 790, 844, 1857
Eam1104I CTCTTC 2 cut(s) 950, 1130
Eam1105I GACNNNNNGTC 1 cut(s) 1662
EarI CTCTTC 2 cut(s) 950, 1130
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 307, 1504
Eco147I AGGCCT 2 cut(s) 342, 1828
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 4 cut(s) 387, 867, 1001, 1664
Eco53kI GAGCTC 1 cut(s) 353
Eco81I CCTNAGG 2 cut(s) 1320, 1969
Eco88I CYCGRG 1 cut(s) 1892
Eco91I GGTNACC 1 cut(s) 1324
EcoICRI GAGCTC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 1324
EcoRI GAATTC 1 cut(s) 452
EcoT14I CCWWGG 2 cut(s) 307, 1504
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 2 cut(s) 307, 1504
FalI AAGNNNNNCTT 2 cut(s) 1872, 1904
FauNDI CATATG 1 cut(s) 1999
FbaI TGATCA 1 cut(s) 1312
Fnu4HI GCNGC 7 cut(s) 516, 519, 522, 821, 1259, 1821, 1869
FokI GGATG 4 cut(s) 739, 796, 1177, 1706
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 7 cut(s) 516, 519, 522, 821, 1259, 1821, 1869
FspBI CTAG 9 cut(s) 26, 35, 992, 1191, 1448, 1472, 1553, 1830, 1905
GlaI GCGC 1 cut(s) 304
GluI GCNGC 7 cut(s) 516, 519, 522, 821, 1259, 1821, 1869
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 9 cut(s) 99, 342, 792, 846, 1433, 1583, 1648, 1828, 1859
HapII CCGG 3 cut(s) 348, 1213, 1877
HhaI GCGC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HindIII AAGCTT 4 cut(s) 161, 829, 1291, 1787
HinfI GANTC 5 cut(s) 312, 556, 1151, 1414, 2009
HpaII CCGG 3 cut(s) 348, 1213, 1877
HphI GGTGA 3 cut(s) 871, 1202, 1336
Hpy166II GTNNAC 2 cut(s) 291, 1922
Hpy188I TCNGA 8 cut(s) 232, 281, 286, 333, 897, 1008, 1740, 1813
Hpy8I GTNNAC 2 cut(s) 291, 1922
Hpy99I CGWCG 2 cut(s) 337, 1601
HpyAV CCTTC 7 cut(s) 208, 229, 262, 945, 1162, 1423, 1461
HpyCH4III ACNGT 4 cut(s) 1029, 1230, 1380, 1928
HpyCH4IV ACGT 1 cut(s) 1599
HpyCH4V TGCA 9 cut(s) 275, 502, 521, 647, 827, 968, 1130, 1490, 2006
HpyF10VI GCNNNNNNNGC 8 cut(s) 521, 638, 837, 1187, 1613, 1834, 1865, 2012
HpyF3I CTNAG 7 cut(s) 116, 229, 299, 554, 1005, 1320, 1969
HpySE526I ACGT 1 cut(s) 1599
HspAI GCGC 1 cut(s) 303
KpnI GGTACC 2 cut(s) 606, 1552
Ksp22I TGATCA 1 cut(s) 1312
KspI CCGCGG 1 cut(s) 841
Kzo9I GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
LmnI GCTCC 9 cut(s) 334, 350, 427, 637, 774, 988, 1255, 1338, 1813
Lsp1109I GCAGC 6 cut(s) 502, 505, 533, 832, 1245, 1855
LweI GCATC 2 cut(s) 1855, 1996
MaeI CTAG 9 cut(s) 26, 35, 992, 1191, 1448, 1472, 1553, 1830, 1905
MaeII ACGT 1 cut(s) 1599
MaeIII GTNAC 7 cut(s) 214, 458, 622, 1208, 1324, 1715, 1884
MalI GATC 6 cut(s) 140, 283, 440, 485, 1314, 1737
MboI GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 4 cut(s) 355, 1566, 1595, 1805
MlsI TGGCCA 3 cut(s) 792, 846, 1859
MluNI TGGCCA 3 cut(s) 792, 846, 1859
MlyI GAGTC 2 cut(s) 565, 2018
MmeI TCCRAC 5 cut(s) 356, 414, 752, 1516, 1685
Mox20I TGGCCA 3 cut(s) 792, 846, 1859
MscI TGGCCA 3 cut(s) 792, 846, 1859
MseI TTAA 6 cut(s) 111, 1014, 1332, 1350, 1653, 1913
MslI CAYNNNNRTG 5 cut(s) 840, 1422, 1598, 1748, 1929
Msp20I TGGCCA 3 cut(s) 792, 846, 1859
MspA1I CMGCKG 3 cut(s) 524, 840, 1820
MspI CCGG 3 cut(s) 348, 1213, 1877
MspR9I CCNGG 1 cut(s) 348
MunI CAATTG 1 cut(s) 636
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 8 cut(s) 521, 638, 837, 1187, 1613, 1834, 1865, 2012
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 2 cut(s) 307, 1504
NdeI CATATG 1 cut(s) 1999
NdeII GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
NlaIV GGNNCC 7 cut(s) 48, 388, 604, 1340, 1550, 1665, 1815
NmeAIII GCCGAG 2 cut(s) 384, 1789
NmuCI GTSAC 4 cut(s) 458, 1208, 1324, 1884
NspI RCATGY 3 cut(s) 827, 1282, 1837
OliI CACNNNNGTG 2 cut(s) 840, 1598
PaeI GCATGC 2 cut(s) 827, 1837
PagI TCATGA 1 cut(s) 1390
PceI AGGCCT 2 cut(s) 342, 1828
PfeI GAWTC 3 cut(s) 312, 1151, 1414
PkrI GCNGC 7 cut(s) 517, 520, 523, 822, 1260, 1822, 1870
PleI GAGTC 2 cut(s) 564, 2017
PpsI GAGTC 2 cut(s) 564, 2017
Psp124BI GAGCTC 1 cut(s) 355
PspEI GGTNACC 1 cut(s) 1324
PspN4I GGNNCC 7 cut(s) 48, 388, 604, 1340, 1550, 1665, 1815
PspPI GGNCC 5 cut(s) 387, 867, 1001, 1581, 1664
PstI CTGCAG 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 9 cut(s) 30, 39, 290, 368, 604, 1019, 1224, 1550, 1853
RsaNI GTAC 9 cut(s) 29, 38, 289, 367, 603, 1018, 1223, 1549, 1852
RseI CAYNNNNRTG 5 cut(s) 840, 1422, 1598, 1748, 1929
SacI GAGCTC 1 cut(s) 355
SacII CCGCGG 1 cut(s) 841
SaqAI TTAA 6 cut(s) 111, 1014, 1332, 1350, 1653, 1913
SatI GCNGC 7 cut(s) 516, 519, 522, 821, 1259, 1821, 1869
Sau3AI GATC 6 cut(s) 138, 281, 438, 483, 1312, 1735
Sau96I GGNCC 5 cut(s) 387, 867, 1001, 1581, 1664
ScaI AGTACT 4 cut(s) 30, 39, 368, 1853
SchI GAGTC 2 cut(s) 565, 2018
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 4 cut(s) 355, 1566, 1595, 1805
SfaNI GCATC 2 cut(s) 1855, 1996
SfcI CTRYAG 1 cut(s) 519
Sfr303I CCGCGG 1 cut(s) 841
SgrBI CCGCGG 1 cut(s) 841
SinI GGWCC 4 cut(s) 387, 867, 1001, 1664
SmiMI CAYNNNNRTG 5 cut(s) 840, 1422, 1598, 1748, 1929
SmlI CTYRAG 3 cut(s) 370, 797, 1724
SmoI CTYRAG 3 cut(s) 370, 797, 1724
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 2 cut(s) 827, 1837
SseBI AGGCCT 2 cut(s) 342, 1828
SsiI CCGC 4 cut(s) 243, 838, 840, 1820
SspMI CTAG 9 cut(s) 26, 35, 992, 1191, 1448, 1472, 1553, 1830, 1905
SstI GAGCTC 1 cut(s) 355
StuI AGGCCT 2 cut(s) 342, 1828
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 2 cut(s) 307, 1504
TaaI ACNGT 4 cut(s) 1029, 1230, 1380, 1928
TaiI ACGT 1 cut(s) 1602
TaqI TCGA 2 cut(s) 486, 1626
TatI WGTACW 7 cut(s) 28, 37, 288, 366, 1017, 1222, 1851
TauI GCSGC 1 cut(s) 1823
TfiI GAWTC 3 cut(s) 312, 1151, 1414
Tru1I TTAA 6 cut(s) 111, 1014, 1332, 1350, 1653, 1913
Tru9I TTAA 6 cut(s) 111, 1014, 1332, 1350, 1653, 1913
TscAI CASTG 6 cut(s) 100, 463, 571, 909, 1137, 1867
TseFI GTSAC 4 cut(s) 458, 1208, 1324, 1884
TseI GCWGC 6 cut(s) 515, 518, 521, 820, 1258, 1868
Tsp45I GTSAC 4 cut(s) 458, 1208, 1324, 1884
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 6 cut(s) 100, 463, 571, 909, 1137, 1867
VpaK11BI GGWCC 4 cut(s) 387, 867, 1001, 1664
XapI RAATTY 5 cut(s) 400, 452, 907, 1172, 1216
XceI RCATGY 3 cut(s) 827, 1282, 1837
XspI CTAG 9 cut(s) 26, 35, 992, 1191, 1448, 1472, 1553, 1830, 1905
ZrmI AGTACT 4 cut(s) 30, 39, 368, 1853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.