Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000256

Physical Location & Seq

Genomic Coordinates Forward (+)

8420054 .. 8422380

Gene Structure 2327 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G009000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGTTTTTCAAGCTTGTAATAATTCTCGTAGTTATTGTCGTAGCAGCAGCTGAAGATCTCAGCTTCATTTACAATGGTTTCGAGTCTGGTGAAGATATTTGGCAAGATGGCCCCGGAGGGGGAGTCATGGTTGATGGTTTATTGAGAATAACGAGTCATACCTTCTACGCCAAGCCAGTAACCTTCAAAAATGACTCATCATCCGGTTCAGAAGTTTTCTCCTTCTCTACCACTTTCGTCTTCGCTATCAGATCAGAATACAAAACTCTCAGCGGCCATGGACTCGCCTTTGTTATCTCTCCAACTCTAGGACGTCCCGGAGCTCTTCCAAGCCAGTACTTGGGCCTTTTCAATGCCTCCAACAATGGGAATATGAGCAATCATGTTTTTGCTGTGGAGCTGGACACGATTCAGAGCGCTGAGTTCAATGACATCAATGAAAATCATGTTGGGATCGACATCAATGGATTGGACTCTGTAAAGTCAGCAACAGCAGGATACTACGTTAAGGACGGAAGGTACCCAAGGAAATTGAGCCTCATCAGTGGAAATCCAATGAAAGTTTGGGTCGAATATGATGGTACCCGGAAGCAAATTAATGTTACTTTGGCTCCGATTAATGTTGGTAAGAAACCCCGAATTCCACTCTTGTCTTTAAAATATGACCTTTCCCCAGTTCTTAACAAAATTATGTATGTTGGCTTCTCCGCATCCACCGGCTCAATTAAAACATTCCATTATATTTTGGGCTGGAGCTTTAGGATGAATGGCCAAGCTCAAGAACTTGTTCCTTCACAGCTTCCCAAATTGCCTAGTATAGGAGACAAGAAAGTGTCTAAACTTCTGACCTTTGGTGTGCCTGTGATGGCTGTGAGTTTAGTTTTGCTAGCGATTTGTCTTGTGGTTTTTGTCATGAGAAGGAAGAAGTACGCAGAACTACTTGAAGATTGGGAGCTCGAGTATGGGCCTCAAAGGTTTAAATACGAAGAATTGTATAAAGCCACTAAAGGGTTTGGAAAGGAAGTGCTCTTGGGTGTTGGGGGATTTGGTGAGGTCTATAGAGGTATCTTACCCACCTCTAAGATTGAGATCGCTGTGAAGAGGGTTTCGCATAAATCAAGACAGGGAATAAAGGAATTTGTAGCCGAAATTGTTAGCCTTGGCCGGCTTCGTCACCGGAATTTAGTACAACTCTTGGGCTATTGCAGGCGAAATGGTGAACTACTTTTGGTTTATGACTACATGCCTAATGGTAGCTTGGAAAAGTACCTCTATAACCAACCGAAGATTACTCTAAACTGGAGTCGGAGGTTTAGAGTCATCAAAGGTGTGGCTTCAGGGTTGTTTTATCTTCATGAAGAGTGGGAACAAGTAGTAATTCACAGAGATGTGAAGGCTAGCAATGTGCTACTAGACCAGGAATTGAATGGAAGACTAGGAGATTTTGGACTTGCAAGATTATGTGCCCATGGGACAGACCATCAAACTACTCATGTAGCTGGCACACTTGGGTATTTAGCCCCGGAGCATGCAAGAACTGGTCGGGCCACCACGAGCACAGACGTGTTTGCTTTCGGGGCATTTTTGCTCGAAGTTGCCTGTGGAAGAAGGCCAATAGAGACACAAGGACCAGAGGAGGATATCATTTTGGCTGATCGGGTTTTCTTGTGTTGGAATAAAGGTAATATTCTAGAGGCAAGAGATCAAAACTTGGGTAACGATTTTGTAGTTGAGGAAGTGGAATTGGTGCTGAAGCTTGCGTTGTTGTGCTCTCGTTCACCGCCTGTAGCTAGGCCAAGCATGCGACAAGTGATGCAGTATTTGGACGGTGATATTGCTCTCCCGGAGTTGTCACTTCTTCGGCTCTCTTCAAGTGGTTCAGCATTTGCGCAAGTTGAAGGTTCTGAGGATGTTGAAATGGCATATAAGTGTTCATACGTTCCAGAGTCGACCCTCCTCTCAGGTGGTCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0010033 GO:0014070 GO:0016020 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0044464 GO:0046677 GO:0048229 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

657

Amino Acids

72.9

Weight (kDa)

8.25

Isoelectric Point (pI)

39.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	20 - 268	9.4e-75	Legume lectin domain
Pkinase	PF00069	341 - 606	1.2e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	343 - 546	5.8e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000256)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07460 AT1G07460
fragaria_vesca	FvH4_6g13200
malus_domestica	MD12G1146600.v1.1 MD12G1147100.v1.1 MD12G1147300.v1.1 MD12G1147500.v1.1
prunus_persica	Prupe.6G259500_v2.0.a1 Prupe.6G259800_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G259900_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G260000_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261400_v2.0.a1 Prupe.6G261500_v2.0.a1
pyrus_communis	pycom04g12150 pycom12g14040 pycom12g14060 pycom12g14080 pycom12g14110
rosa_chinensis	RchiOBHm_Chr3g0460311 RchiOBHm_Chr3g0465181 RchiOBHm_Chr3g0465701 RchiOBHm_Chr3g0465801 RchiOBHm_Chr3g0465861 RchiOBHm_Chr3g0465911 RchiOBHm_Chr3g0465931 RchiOBHm_Chr3g0465991 RchiOBHm_Chr3g0466011 RchiOBHm_Chr4g0404151 RchiOBHm_Chr6g0253601 RchiOBHm_Chr6g0259671 RchiOBHm_Chr7g0189621 RchiOBHm_Chr7g0189631 RchiOBHm_Chr7g0216481
rosa_laevigata	RLG00000014514 RLG00000014515 RLG00000014999 RLG00000024609 RLG00000024614 RLG00000024616 RLG00000024620 RLG00000024624 RLG00000024627 RLG00000024650 RLG00000025025
rosa_multiflora	Rmu_co8340115.1_g000001 Rmu_sc0000362.1_g000006 Rmu_sc0000449.1_g000013 Rmu_sc0000536.1_g000003 Rmu_sc0000536.1_g000006 Rmu_sc0002405.1_g000009 Rmu_sc0003391.1_g000002 Rmu_sc0003391.1_g000003 Rmu_sc0003391.1_g000013 Rmu_sc0003492.1_g000011 Rmu_sc0004305.1_g000015 Rmu_sc0004305.1_g000025 Rmu_sc0007705.1_g000012 Rmu_sc0007705.1_g000014 Rmu_sc0007705.1_g000015 Rmu_sc0007705.1_g000018 Rmu_sc0009386.1_g000006 Rmu_sc0009386.1_g000008 Rmu_sc0010030.1_g000001 Rmu_sc0010217.1_g000002 Rmu_sc0034485.1_g000001 Rmu_ssc0000454.1_g000025
rosa_roxburghii	Rroxscaffold_3G00243190 Rroxscaffold_6G00413130 Rroxscaffold_6G00414850 Rroxscaffold_6G00414890 Rroxscaffold_6G00414910 Rroxscaffold_6G00414930 Rroxscaffold_6G00419650 Rroxscaffold_7G00206940
rosa_rugosa	Rorug03G0041500 Rorug03G0078300 Rorug03G0078500 Rorug03G0078600 Rorug03G0078800 Rorug03G0078900 Rorug07G0160500
rosa_samantha	Rh3BG103300 Rh3BG139800 Rh3BG143800 Rh3BG144300 Rh3BG145000 Rh3BG145500 Rh3BG145600 Rh3BG146000 Rh3CG104900 Rh3CG141500 Rh3CG145100 Rh3CG145800 Rh3CG146300 Rh3CG146500 Rh3CG146700 Rh3CG147000 Rh3CG149900 Rh3DG104600 Rh3DG141000 Rh3DG145100 Rh3DG145600 Rh3DG146100 Rh3DG146400 Rh3DG146600 Rh3DG146900 Rh4AG123200 Rh4BG116400 Rh6AG060500 Rh6BG101000 Rh7AG299800 Rh7BG291300 Rh7DG106700 Rh7DG106900
rosa_wichuraiana	Rw3G008440 Rw3G011450 Rw3G011670 Rw3G011710 Rw3G011720 Rw3G011750 Rw3G011770 Rw3G011810 Rw6G005390 Rw7G009000 Rw7G025480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	316
Acc16I	TGCGCA	1 cut(s)	1890
Acc65I	GGTACC	2 cut(s)	519, 581
AccB1I	GGYRCC	2 cut(s)	519, 581
AccB7I	CCANNNNNTGG	1 cut(s)	340
AccI	GTMKAC	1 cut(s)	1949
AciI	CCGC	3 cut(s)	273, 708, 1781
AclWI	GGATC	1 cut(s)	461
AcoI	YGGCCR	3 cut(s)	274, 769, 1162
AcsI	RAATTY	3 cut(s)	639, 1136, 1180
AcuI	CTGAAG	3 cut(s)	72, 1320, 1772
AcyI	GRCGYC	1 cut(s)	313
AfaI	GTAC	6 cut(s)	338, 521, 583, 929, 1188, 1268
AfeI	AGCGCT	1 cut(s)	418
AfiI	CCNNNNNNNGG	7 cut(s)	118, 119, 308, 340, 366, 818, 1008
AflIII	ACRYGT	1 cut(s)	1563
AgsI	TTSAA	9 cut(s)	10, 187, 352, 427, 944, 1426, 1872, 1898, 1916
AjiI	CACGTC	1 cut(s)	1564
AjnI	CCWGG	1 cut(s)	1416
AjuI	GAANNNNNNNTTGG	2 cut(s)	432, 464
AleI	CACNNNNGTG	1 cut(s)	1562
Alw21I	GWGCWC	5 cut(s)	325, 957, 1029, 1559, 1772
Alw26I	GTCTC	2 cut(s)	816, 1613
AlwI	GGATC	1 cut(s)	461
AlwNI	CAGNNNCTG	1 cut(s)	50
Ama87I	CYCGRG	1 cut(s)	956
Aor51HI	AGCGCT	1 cut(s)	418
AoxI	GGCC	9 cut(s)	109, 274, 343, 769, 965, 1162, 1545, 1610, 1793
ApeKI	GCWGC	2 cut(s)	44, 47
ApoI	RAATTY	3 cut(s)	639, 1136, 1180
AseI	ATTAAT	2 cut(s)	597, 618
Asp700I	GAANNNNTTC	1 cut(s)	786
Asp718I	GGTACC	2 cut(s)	519, 581
AspLEI	GCGC	2 cut(s)	419, 1891
AspS9I	GGNCC	5 cut(s)	110, 343, 965, 1545, 1628
AsuC2I	CCSGG	5 cut(s)	114, 318, 586, 1523, 1844
AsuHPI	GGTGA	6 cut(s)	101, 1061, 1166, 1229, 1770, 1841
AsuNHI	GCTAGC	2 cut(s)	886, 1397
AvaI	CYCGRG	1 cut(s)	956
AvaII	GGWCC	1 cut(s)	1628
BaeGI	GKGCMC	1 cut(s)	1468
BalI	TGGCCA	1 cut(s)	771
BanI	GGYRCC	2 cut(s)	519, 581
BanII	GRGCYC	2 cut(s)	325, 957
BarI	GAAGNNNNNNTAC	2 cut(s)	686, 718
BauI	CACGAG	1 cut(s)	1552
BbsI	GAAGAC	2 cut(s)	232, 1438
Bbv12I	GWGCWC	5 cut(s)	325, 957, 1029, 1559, 1772
BbvI	GCAGC	2 cut(s)	56, 59
BccI	CCATC	5 cut(s)	101, 128, 572, 859, 1488
BciT130I	CCWGG	1 cut(s)	1418
BciVI	GTATCC	1 cut(s)	491
BcnI	CCSGG	5 cut(s)	114, 318, 586, 1523, 1844
BcoDI	GTCTC	2 cut(s)	816, 1613
BfaI	CTAG	8 cut(s)	308, 813, 887, 1398, 1412, 1436, 1691, 1791
BfmI	CTRYAG	2 cut(s)	1057, 1785
BfoI	RGCGCY	1 cut(s)	420
BfuI	GTATCC	1 cut(s)	491
BglII	AGATCT	1 cut(s)	55
BisI	GCNGC	3 cut(s)	45, 48, 274
BlsI	GCNGC	3 cut(s)	46, 49, 275
BmcAI	AGTACT	1 cut(s)	338
Bme1390I	CCNGG	6 cut(s)	114, 318, 586, 1418, 1523, 1844
Bme18I	GGWCC	1 cut(s)	1628
BmeT110I	CYCGRG	1 cut(s)	956
BmgBI	CACGTC	1 cut(s)	1564
BmgT120I	GGNCC	5 cut(s)	110, 343, 965, 1545, 1628
BmiI	GGNNCC	4 cut(s)	112, 521, 583, 612
BmrFI	CCNGG	6 cut(s)	114, 318, 586, 1418, 1523, 1844
BmrI	ACTGGG	1 cut(s)	668
BmsI	GCATC	2 cut(s)	719, 1803
BmtI	GCTAGC	2 cut(s)	890, 1401
BmuI	ACTGGG	1 cut(s)	668
BpiI	GAAGAC	2 cut(s)	232, 1438
BpmI	CTGGAG	2 cut(s)	772, 1321
BpuEI	CTTGAG	1 cut(s)	762
BpuMI	CCSGG	5 cut(s)	114, 318, 586, 1523, 1844
BsaBI	GATNNNNATC	2 cut(s)	458, 1088
BsaHI	GRCGYC	1 cut(s)	313
BsaJI	CCNNGG	6 cut(s)	112, 277, 524, 1159, 1468, 1521
BsaWI	WCCGGW	2 cut(s)	203, 1176
BsaXI	ACNNNNNCTCC	8 cut(s)	108, 114, 138, 144, 595, 625, 944, 974
Bsc4I	CCNNNNNNNGG	7 cut(s)	118, 119, 308, 340, 366, 818, 1008
Bse118I	RCCGGY	2 cut(s)	716, 1164
Bse1I	ACTGG	5 cut(s)	176, 334, 674, 1304, 1543
Bse3DI	GCAATG	1 cut(s)	1408
Bse8I	GATNNNNATC	2 cut(s)	458, 1088
BseBI	CCWGG	1 cut(s)	1418
BseDI	CCNNGG	6 cut(s)	112, 277, 524, 1159, 1468, 1521
BseGI	GGATG	4 cut(s)	200, 710, 768, 1915
BseJI	GATNNNNATC	2 cut(s)	458, 1088
BseLI	CCNNNNNNNGG	7 cut(s)	118, 119, 308, 340, 366, 818, 1008
BseMI	GCAATG	1 cut(s)	1408
BseMII	CTCAG	5 cut(s)	73, 283, 411, 1896, 1974
BseNI	ACTGG	5 cut(s)	176, 334, 674, 1304, 1543
BseRI	GAGGAG	2 cut(s)	1649, 1946
BseSI	GKGCMC	1 cut(s)	1468
BseXI	GCAGC	2 cut(s)	56, 59
BshFI	GGCC	9 cut(s)	111, 276, 345, 771, 967, 1164, 1547, 1612, 1795
BshNI	GGYRCC	2 cut(s)	519, 581
BsiHKAI	GWGCWC	5 cut(s)	325, 957, 1029, 1559, 1772
BsiHKCI	CYCGRG	1 cut(s)	956
BsiSI	CCGG	9 cut(s)	114, 204, 318, 586, 717, 1165, 1177, 1523, 1844
BslFI	GGGAC	2 cut(s)	300, 1486
BslI	CCNNNNNNNGG	7 cut(s)	118, 119, 308, 340, 366, 818, 1008
BsmAI	GTCTC	2 cut(s)	816, 1613
BsmFI	GGGAC	2 cut(s)	300, 1486
BsnI	GGCC	9 cut(s)	111, 276, 345, 771, 967, 1164, 1547, 1612, 1795
BsoBI	CYCGRG	1 cut(s)	956
Bsp1286I	GDGCHC	6 cut(s)	325, 957, 1029, 1468, 1559, 1772
Bsp143I	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
Bsp19I	CCATGG	2 cut(s)	277, 1468
BspACI	CCGC	3 cut(s)	273, 708, 1781
BspANI	GGCC	9 cut(s)	111, 276, 345, 771, 967, 1164, 1547, 1612, 1795
BspCNI	CTCAG	5 cut(s)	72, 282, 412, 1897, 1973
BspHI	TCATGA	2 cut(s)	912, 1354
BspLI	GGNNCC	4 cut(s)	112, 521, 583, 612
BspOI	GCTAGC	2 cut(s)	890, 1401
BspPI	GGATC	1 cut(s)	461
BspQI	GCTCTTC	1 cut(s)	330
BspT107I	GGYRCC	2 cut(s)	519, 581
BsrDI	GCAATG	1 cut(s)	1408
BsrFI	RCCGGY	2 cut(s)	716, 1164
BsrI	ACTGG	5 cut(s)	176, 334, 674, 1304, 1543
BssAI	RCCGGY	2 cut(s)	716, 1164
BssECI	CCNNGG	6 cut(s)	112, 277, 524, 1159, 1468, 1521
BssMI	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
BssNI	GRCGYC	1 cut(s)	313
BssSI	CACGAG	1 cut(s)	1552
BssT1I	CCWWGG	4 cut(s)	277, 524, 1159, 1468
Bst2BI	CACGAG	1 cut(s)	1552
Bst2UI	CCWGG	1 cut(s)	1418
Bst4CI	ACNGT	1 cut(s)	1829
Bst6I	CTCTTC	4 cut(s)	330, 1094, 1353, 1873
BstACI	GRCGYC	1 cut(s)	313
BstC8I	GCNNGC	8 cut(s)	888, 1166, 1208, 1399, 1501, 1530, 1758, 1802
BstDEI	CTNAG	6 cut(s)	59, 269, 420, 1080, 1905, 1960
BstDSI	CCRYGG	2 cut(s)	277, 1468
BstENI	CCTNNNNNAGG	1 cut(s)	816
BstF5I	GGATG	4 cut(s)	200, 710, 768, 1915
BstH2I	RGCGCY	1 cut(s)	420
BstHHI	GCGC	2 cut(s)	419, 1891
BstKTI	GATC	6 cut(s)	58, 254, 456, 1092, 1657, 1705
BstMAI	GTCTC	2 cut(s)	816, 1613
BstMBI	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
BstMWI	GCNNNNNNNGC	2 cut(s)	1577, 1801
BstNI	CCWGG	1 cut(s)	1418
BstNSI	RCATGY	3 cut(s)	1246, 1532, 1804
BstSCI	CCNGG	6 cut(s)	112, 316, 584, 1416, 1521, 1842
BstSFI	CTRYAG	2 cut(s)	1057, 1785
BstSLI	GKGCMC	1 cut(s)	1468
BstV1I	GCAGC	2 cut(s)	56, 59
BstV2I	GAAGAC	2 cut(s)	232, 1438
BstX2I	RGATCY	1 cut(s)	55
BstYI	RGATCY	1 cut(s)	55
BsuI	GTATCC	1 cut(s)	491
BsuRI	GGCC	9 cut(s)	111, 276, 345, 771, 967, 1164, 1547, 1612, 1795
BtgI	CCRYGG	2 cut(s)	277, 1468
BtrI	CACGTC	1 cut(s)	1564
BtsCI	GGATG	4 cut(s)	200, 710, 768, 1915
BtsIMutI	CAGTG	1 cut(s)	550
Cac8I	GCNNGC	8 cut(s)	888, 1166, 1208, 1399, 1501, 1530, 1758, 1802
CaiI	CAGNNNCTG	1 cut(s)	50
CciI	TCATGA	2 cut(s)	912, 1354
CfoI	GCGC	2 cut(s)	419, 1891
Cfr10I	RCCGGY	2 cut(s)	716, 1164
Cfr13I	GGNCC	5 cut(s)	110, 343, 965, 1545, 1628
Csp6I	GTAC	6 cut(s)	337, 520, 582, 928, 1187, 1267
CviQI	GTAC	6 cut(s)	337, 520, 582, 928, 1187, 1267
DdeI	CTNAG	6 cut(s)	59, 269, 420, 1080, 1905, 1960
DpnI	GATC	6 cut(s)	57, 253, 455, 1091, 1656, 1704
DpnII	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
DraI	TTTAAA	2 cut(s)	657, 979
EaeI	YGGCCR	3 cut(s)	274, 769, 1162
Eam1104I	CTCTTC	4 cut(s)	330, 1094, 1353, 1873
EarI	CTCTTC	4 cut(s)	330, 1094, 1353, 1873
Ecl136II	GAGCTC	2 cut(s)	323, 955
Eco130I	CCWWGG	4 cut(s)	277, 524, 1159, 1468
Eco24I	GRGCYC	2 cut(s)	325, 957
Eco32I	GATATC	1 cut(s)	1642
Eco47I	GGWCC	1 cut(s)	1628
Eco47III	AGCGCT	1 cut(s)	418
Eco53kI	GAGCTC	2 cut(s)	323, 955
Eco57I	CTGAAG	3 cut(s)	72, 1320, 1772
Eco88I	CYCGRG	1 cut(s)	956
EcoICRI	GAGCTC	2 cut(s)	323, 955
EcoNI	CCTNNNNNAGG	1 cut(s)	816
EcoRI	GAATTC	1 cut(s)	639
EcoRII	CCWGG	1 cut(s)	1416
EcoRV	GATATC	1 cut(s)	1642
EcoT14I	CCWWGG	4 cut(s)	277, 524, 1159, 1468
EcoT38I	GRGCYC	2 cut(s)	325, 957
ErhI	CCWWGG	4 cut(s)	277, 524, 1159, 1468
FaqI	GGGAC	2 cut(s)	300, 1486
FblI	GTMKAC	1 cut(s)	1949
Fnu4HI	GCNGC	3 cut(s)	45, 48, 274
FokI	GGATG	4 cut(s)	187, 697, 775, 1922
FriOI	GRGCYC	2 cut(s)	325, 957
Fsp4HI	GCNGC	3 cut(s)	45, 48, 274
FspBI	CTAG	8 cut(s)	308, 813, 887, 1398, 1412, 1436, 1691, 1791
FspI	TGCGCA	1 cut(s)	1890
GlaI	GCGC	2 cut(s)	418, 1890
GluI	GCNGC	3 cut(s)	45, 48, 274
GsuI	CTGGAG	2 cut(s)	772, 1321
HaeII	RGCGCY	1 cut(s)	420
HaeIII	GGCC	9 cut(s)	111, 276, 345, 771, 967, 1164, 1547, 1612, 1795
HapII	CCGG	9 cut(s)	114, 204, 318, 586, 717, 1165, 1177, 1523, 1844
HhaI	GCGC	2 cut(s)	419, 1891
Hin1I	GRCGYC	1 cut(s)	313
Hin6I	GCGC	2 cut(s)	417, 1889
HinP1I	GCGC	2 cut(s)	417, 1889
HincII	GTYRAC	1 cut(s)	1950
HindII	GTYRAC	1 cut(s)	1950
HindIII	AAGCTT	2 cut(s)	11, 1754
HpaII	CCGG	9 cut(s)	114, 204, 318, 586, 717, 1165, 1177, 1523, 1844
HphI	GGTGA	6 cut(s)	101, 1061, 1166, 1229, 1770, 1841
Hpy166II	GTNNAC	3 cut(s)	1220, 1778, 1950
Hpy188I	TCNGA	8 cut(s)	211, 251, 256, 414, 615, 846, 1308, 1906
Hpy188III	TCNNGA	6 cut(s)	779, 913, 1119, 1355, 1691, 1943
Hpy8I	GTNNAC	3 cut(s)	1220, 1778, 1950
HpyAV	CCTTC	9 cut(s)	172, 193, 232, 510, 801, 912, 1387, 1602, 1892
HpyCH4III	ACNGT	1 cut(s)	1829
HpyCH4IV	ACGT	4 cut(s)	313, 504, 1563, 1938
HpyCH4V	TGCA	4 cut(s)	1206, 1454, 1532, 1816
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1577, 1801
HpyF3I	CTNAG	6 cut(s)	59, 269, 420, 1080, 1905, 1960
HpySE526I	ACGT	4 cut(s)	313, 504, 1563, 1938
Hsp92I	GRCGYC	1 cut(s)	313
HspAI	GCGC	2 cut(s)	417, 1889
KpnI	GGTACC	2 cut(s)	523, 585
KroI	GCCGGC	1 cut(s)	1164
KroNI	GCCGGC	1 cut(s)	1166
Kzo9I	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
LguI	GCTCTTC	1 cut(s)	330
LmnI	GCTCC	6 cut(s)	320, 397, 616, 753, 952, 1525
Lsp1109I	GCAGC	2 cut(s)	56, 59
LweI	GCATC	2 cut(s)	719, 1803
MaeI	CTAG	8 cut(s)	308, 813, 887, 1398, 1412, 1436, 1691, 1791
MaeII	ACGT	4 cut(s)	313, 504, 1563, 1938
MaeIII	GTNAC	5 cut(s)	178, 601, 1172, 1715, 1851
MalI	GATC	6 cut(s)	57, 253, 455, 1091, 1656, 1704
MboI	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
MflI	RGATCY	1 cut(s)	55
MhlI	GDGCHC	6 cut(s)	325, 957, 1029, 1468, 1559, 1772
MlsI	TGGCCA	1 cut(s)	771
MluNI	TGGCCA	1 cut(s)	771
MlyI	GAGTC	9 cut(s)	92, 132, 163, 188, 276, 467, 1312, 1326, 1955
MmeI	TCCRAC	4 cut(s)	326, 384, 1286, 1652
Mox20I	TGGCCA	1 cut(s)	771
MroNI	GCCGGC	1 cut(s)	1164
MroXI	GAANNNNTTC	1 cut(s)	786
MscI	TGGCCA	1 cut(s)	771
MseI	TTAA	7 cut(s)	507, 597, 618, 656, 681, 726, 978
MslI	CAYNNNNRTG	3 cut(s)	1386, 1562, 1927
Msp20I	TGGCCA	1 cut(s)	771
MspA1I	CMGCKG	2 cut(s)	50, 273
MspI	CCGG	9 cut(s)	114, 204, 318, 586, 717, 1165, 1177, 1523, 1844
MspR9I	CCNGG	6 cut(s)	114, 318, 586, 1418, 1523, 1844
MvaI	CCWGG	1 cut(s)	1418
MwoI	GCNNNNNNNGC	2 cut(s)	1577, 1801
NaeI	GCCGGC	1 cut(s)	1166
NciI	CCSGG	5 cut(s)	114, 318, 586, 1523, 1844
NcoI	CCATGG	2 cut(s)	277, 1468
NdeII	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
NgoMIV	GCCGGC	1 cut(s)	1164
NheI	GCTAGC	2 cut(s)	886, 1397
NlaIV	GGNNCC	4 cut(s)	112, 521, 583, 612
NmuCI	GTSAC	2 cut(s)	1172, 1851
NsbI	TGCGCA	1 cut(s)	1890
NspI	RCATGY	3 cut(s)	1246, 1532, 1804
OliI	CACNNNNGTG	1 cut(s)	1562
PaeI	GCATGC	2 cut(s)	1532, 1804
PaeR7I	CTCGAG	1 cut(s)	956
PagI	TCATGA	2 cut(s)	912, 1354
PciSI	GCTCTTC	1 cut(s)	330
PdiI	GCCGGC	1 cut(s)	1166
PdmI	GAANNNNTTC	1 cut(s)	786
PfeI	GAWTC	1 cut(s)	409
PflMI	CCANNNNNTGG	1 cut(s)	340
PfoI	TCCNGGA	2 cut(s)	316, 1842
PkrI	GCNGC	3 cut(s)	46, 49, 275
PleI	GAGTC	9 cut(s)	91, 131, 162, 188, 276, 467, 1311, 1325, 1954
PpsI	GAGTC	9 cut(s)	91, 131, 162, 188, 276, 467, 1311, 1325, 1954
PshBI	ATTAAT	2 cut(s)	597, 618
Psp124BI	GAGCTC	2 cut(s)	325, 957
Psp6I	CCWGG	1 cut(s)	1416
PspGI	CCWGG	1 cut(s)	1416
PspN4I	GGNNCC	4 cut(s)	112, 521, 583, 612
PspPI	GGNCC	5 cut(s)	110, 343, 965, 1545, 1628
PspXI	VCTCGAGB	1 cut(s)	956
PstNI	CAGNNNCTG	1 cut(s)	50
PsuI	RGATCY	1 cut(s)	55
PvuII	CAGCTG	1 cut(s)	50
RsaI	GTAC	6 cut(s)	338, 521, 583, 929, 1188, 1268
RsaNI	GTAC	6 cut(s)	337, 520, 582, 928, 1187, 1267
RseI	CAYNNNNRTG	3 cut(s)	1386, 1562, 1927
SacI	GAGCTC	2 cut(s)	325, 957
SalI	GTCGAC	1 cut(s)	1948
SapI	GCTCTTC	1 cut(s)	330
SaqAI	TTAA	7 cut(s)	507, 597, 618, 656, 681, 726, 978
SatI	GCNGC	3 cut(s)	45, 48, 274
Sau3AI	GATC	6 cut(s)	55, 251, 453, 1089, 1654, 1702
Sau96I	GGNCC	5 cut(s)	110, 343, 965, 1545, 1628
ScaI	AGTACT	1 cut(s)	338
SchI	GAGTC	9 cut(s)	92, 132, 163, 188, 276, 467, 1312, 1326, 1955
ScrFI	CCNGG	6 cut(s)	114, 318, 586, 1418, 1523, 1844
SduI	GDGCHC	6 cut(s)	325, 957, 1029, 1468, 1559, 1772
SfaNI	GCATC	2 cut(s)	719, 1803
SfcI	CTRYAG	2 cut(s)	1057, 1785
Sfr274I	CTCGAG	1 cut(s)	956
SinI	GGWCC	1 cut(s)	1628
SlaI	CTCGAG	1 cut(s)	956
SmiMI	CAYNNNNRTG	3 cut(s)	1386, 1562, 1927
SmlI	CTYRAG	2 cut(s)	777, 956
SmoI	CTYRAG	2 cut(s)	777, 956
SphI	GCATGC	2 cut(s)	1532, 1804
SsiI	CCGC	3 cut(s)	273, 708, 1781
SspI	AATATT	1 cut(s)	1687
SspMI	CTAG	8 cut(s)	308, 813, 887, 1398, 1412, 1436, 1691, 1791
SstI	GAGCTC	2 cut(s)	325, 957
StyD4I	CCNGG	6 cut(s)	112, 316, 584, 1416, 1521, 1842
StyI	CCWWGG	4 cut(s)	277, 524, 1159, 1468
TaaI	ACNGT	1 cut(s)	1829
TaiI	ACGT	4 cut(s)	316, 507, 1566, 1941
TaqI	TCGA	6 cut(s)	81, 456, 570, 957, 1590, 1949
TatI	WGTACW	2 cut(s)	336, 1186
TauI	GCSGC	1 cut(s)	276
TfiI	GAWTC	1 cut(s)	409
Tru1I	TTAA	7 cut(s)	507, 597, 618, 656, 681, 726, 978
Tru9I	TTAA	7 cut(s)	507, 597, 618, 656, 681, 726, 978
TscAI	CASTG	1 cut(s)	550
TseFI	GTSAC	2 cut(s)	1172, 1851
TseI	GCWGC	2 cut(s)	44, 47
Tsp45I	GTSAC	2 cut(s)	1172, 1851
TspDTI	ATGAA	7 cut(s)	55, 453, 572, 779, 1343, 1371, 1923
TspGWI	ACGGA	1 cut(s)	528
TspRI	CASTG	1 cut(s)	550
Van91I	CCANNNNNTGG	1 cut(s)	340
VpaK11BI	GGWCC	1 cut(s)	1628
VspI	ATTAAT	2 cut(s)	597, 618
XagI	CCTNNNNNAGG	1 cut(s)	816
XapI	RAATTY	3 cut(s)	639, 1136, 1180
XbaI	TCTAGA	1 cut(s)	1690
XceI	RCATGY	3 cut(s)	1246, 1532, 1804
XcmI	CCANNNNNNNNNTGG	2 cut(s)	561, 1424
XhoI	CTCGAG	1 cut(s)	956
XmiI	GTMKAC	1 cut(s)	1949
XmnI	GAANNNNTTC	1 cut(s)	786
XspI	CTAG	8 cut(s)	308, 813, 887, 1398, 1412, 1436, 1691, 1791
ZraI	GACGTC	1 cut(s)	314
ZrmI	AGTACT	1 cut(s)	338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.