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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 6 |
| AcsI | RAATTY | 1 cut(s) | 134 |
| AflII | CTTAAG | 1 cut(s) | 127 |
| AluBI | AGCT | 2 cut(s) | 109, 118 |
| AluI | AGCT | 2 cut(s) | 109, 118 |
| AoxI | GGCC | 1 cut(s) | 90 |
| ApoI | RAATTY | 1 cut(s) | 134 |
| BccI | CCATC | 1 cut(s) | 17 |
| BfaI | CTAG | 2 cut(s) | 119, 154 |
| BfrI | CTTAAG | 1 cut(s) | 127 |
| Bse3DI | GCAATG | 1 cut(s) | 27 |
| BseGI | GGATG | 1 cut(s) | 148 |
| BseMI | GCAATG | 1 cut(s) | 27 |
| BshFI | GGCC | 1 cut(s) | 92 |
| BsnI | GGCC | 1 cut(s) | 92 |
| BspANI | GGCC | 1 cut(s) | 92 |
| BspTI | CTTAAG | 1 cut(s) | 127 |
| BsrDI | GCAATG | 1 cut(s) | 27 |
| BstAFI | CTTAAG | 1 cut(s) | 127 |
| BstC8I | GCNNGC | 1 cut(s) | 111 |
| BstDEI | CTNAG | 1 cut(s) | 10 |
| BstF5I | GGATG | 1 cut(s) | 148 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 110, 115 |
| BsuRI | GGCC | 1 cut(s) | 92 |
| BtsCI | GGATG | 1 cut(s) | 148 |
| Cac8I | GCNNGC | 1 cut(s) | 111 |
| CviAII | CATG | 1 cut(s) | 94 |
| CviJI | RGCY | 4 cut(s) | 92, 104, 109, 118 |
| CviKI_1 | RGCY | 4 cut(s) | 92, 104, 109, 118 |
| DdeI | CTNAG | 1 cut(s) | 10 |
| FaeI | CATG | 1 cut(s) | 97 |
| FaiI | YATR | 4 cut(s) | 59, 61, 95, 148 |
| FatI | CATG | 1 cut(s) | 93 |
| FblI | GTMKAC | 1 cut(s) | 6 |
| FspBI | CTAG | 2 cut(s) | 119, 154 |
| HaeIII | GGCC | 1 cut(s) | 92 |
| Hin1II | CATG | 1 cut(s) | 97 |
| HindIII | AAGCTT | 1 cut(s) | 107 |
| HinfI | GANTC | 1 cut(s) | 122 |
| Hpy166II | GTNNAC | 1 cut(s) | 7 |
| Hpy188III | TCNNGA | 1 cut(s) | 47 |
| Hpy8I | GTNNAC | 1 cut(s) | 7 |
| HpyAV | CCTTC | 1 cut(s) | 28 |
| HpyCH4V | TGCA | 2 cut(s) | 63, 113 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 110, 115 |
| HpyF3I | CTNAG | 1 cut(s) | 10 |
| Hsp92II | CATG | 1 cut(s) | 97 |
| LpnPI | CCDG | 1 cut(s) | 32 |
| MaeI | CTAG | 2 cut(s) | 119, 154 |
| MluCI | AATT | 1 cut(s) | 134 |
| MlyI | GAGTC | 1 cut(s) | 116 |
| MnlI | CCTC | 1 cut(s) | 82 |
| MseI | TTAA | 1 cut(s) | 128 |
| MspCI | CTTAAG | 1 cut(s) | 127 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 110, 115 |
| NlaIII | CATG | 1 cut(s) | 97 |
| PleI | GAGTC | 1 cut(s) | 116 |
| PpsI | GAGTC | 1 cut(s) | 116 |
| SaqAI | TTAA | 1 cut(s) | 128 |
| SchI | GAGTC | 1 cut(s) | 116 |
| SetI | ASST | 4 cut(s) | 39, 77, 111, 120 |
| SgeI | CNNG | 7 cut(s) | 27, 59, 84, 106, 118, 122, 131 |
| SmlI | CTYRAG | 1 cut(s) | 127 |
| SmoI | CTYRAG | 1 cut(s) | 127 |
| Sse9I | AATT | 1 cut(s) | 134 |
| SspMI | CTAG | 2 cut(s) | 119, 154 |
| TasI | AATT | 1 cut(s) | 134 |
| Tru1I | TTAA | 1 cut(s) | 128 |
| Tru9I | TTAA | 1 cut(s) | 128 |
| TspDTI | ATGAA | 1 cut(s) | 110 |
| Vha464I | CTTAAG | 1 cut(s) | 127 |
| XapI | RAATTY | 1 cut(s) | 134 |
| XmiI | GTMKAC | 1 cut(s) | 6 |
| XspI | CTAG | 2 cut(s) | 119, 154 |