Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0002556

Physical Location & Seq

Genomic Coordinates Forward (+)

67513760 .. 67518045

Gene Structure 4286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015526

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGAAGCTCACTTTCGCGACGAAAGAGATTGATGTAACGGAATGGAAAGGGGGCCTGCTTGCGTTTGGTGTTACAGAGAAGGACTTGAGGAAAGATCAGAACTCGAAATTCGAAAATCTGATATTGAAGAAGCTGGATTCTTATTTGGGTGGGGTGTTGGCTGAGGTCTCGACTAAGGAGGACTACACAGGAAAGTCTGGCCAGTCTACGGTTATTAGGCTTCCTGGTCTTGGCTCGAAGAGGGTCGGGCTTGCCCAAGCAACGGAGCCCGCTGCTGTTCGGTTCACGGCAATGGAAACAGTTGCAGGCGGAGGATCCGAGAGGCGGAGAAGGTCGGGGTTCAACAAGTCTGAGTCAGCGATCGAAATCTGGGTTTGGGTCGAAGGACGCCGGAGATTGACGTCAGCTTCTGTTCTGTCACGATCCGATTCGGATGTCTTGGTACTGAAGTTGGAATCGATCACTGCCGGAATGGTGAGAAAGGAATCAAGTGGTGGCTACAACATCAAGACAGGACCTGGCTGTTCAATTGAGCTTATGAAGGTTGATATGAGAGGTTCAGCAAAAGCCATTGGTCAAATCAAACCTTCTGGTGTAGAGGCGCATTTCATTGTCGTAGCTTGTGAGAATATGATAAGTGGAACAGGGATGAGGCCTGGGGACATTGTCACAGCTTCTAATGGAAAGACCATTGAGGTCAATAACACAGATGTTGAAGGCAGACTGACTTTGGCAGATGCATTAGTATATGCATCAGGGATTCCGCATGTCAGGCAAATGTTTTATCATGCATTTAATGGTTACATGGACCATGTTTTCCCACTTGATGAATTAAGGCCTCATTCTTGTGGGGGTGAAGATAGCCTTGGTGGATATGCCCCAACTTTGATTGATGCTTTAGAGACACTAGTTTTACTTGGTGATCGGGGCGGCTTTGCTACCTCTGATGAATGGATTGGTAAAAACCTTCAGTTTAAAATAAATAAGACGAAGAGTTTTCTGACCCTATCAATGGCGTTACATCCCCTGTTTAGATACCTATTATTAGAAAGATGGCTCTGCAAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006508 GO:0006807 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0010035 GO:0010038 GO:0010150 GO:0016787 GO:0016805 GO:0019538 GO:0030145 GO:0032501 GO:0032502 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0070011 GO:0071704 GO:0090693 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko00480 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map00480 map01100 map03020 map05016 map05169

Pfam Domains

Protein Families

Peptidase_M17 Peptidase_M17_N

Protein Analysis

357

Amino Acids

38.82

Weight (kDa)

8.73

Isoelectric Point (pI)

43.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M17	PF00883	164 - 252	3.1e-28	Cytosol aminopeptidase family, catalytic domain
Glyco_hydro_47	PF01532	260 - 330	1.4e-15	Glycosyl hydrolase family 47

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002556)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr4g0410691 RchiOBHm_Chr4g0410701 RchiOBHm_Chr5g0035291
rosa_laevigata	RLG00000001607 RLG00000015526 RLG00000023019 RLG00000036605
rosa_multiflora	Rmu_co8131160.1_g000001 Rmu_sc0001785.1_g000010 Rmu_sc0001814.1_g000016 Rmu_sc0001826.1_g000001 Rmu_sc0002000.1_g000002 Rmu_sc0003651.1_g000005 Rmu_sc0011831.1_g000005 Rmu_sc0012973.1_g000002
rosa_roxburghii	Rroxscaffold_4G00299710 Rroxscaffold_7G00196790 Rroxscaffold_7G00217370
rosa_rugosa	Rorug03G0290600 Rorug03G0290700
rosa_samantha	Rh1DG080800 Rh2AG462100 Rh2DG335900 Rh2DG385700 Rh4AG012500 Rh4AG162400 Rh4AG162500 Rh4CG268800 Rh4CG268900 Rh5AG233100 Rh5AG277300 Rh6AG324000 Rh6AG324100 Rh6BG111500 Rh6BG138200 Rh6BG138300 Rh6CG109200 Rh6CG109300 Rh6CG135800
rosa_wichuraiana	Rw4G004860 Rw6G028100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	404
AccI	GTMKAC	1 cut(s)	206
AccII	CGCG	1 cut(s)	17
AciI	CCGC	5 cut(s)	270, 309, 325, 764, 930
AclWI	GGATC	3 cut(s)	309, 322, 417
AcoI	YGGCCR	1 cut(s)	199
AcsI	RAATTY	1 cut(s)	107
AcuI	CTGAAG	2 cut(s)	467, 953
AcyI	GRCGYC	2 cut(s)	388, 401
AfaI	GTAC	1 cut(s)	444
AfiI	CCNNNNNNNGG	5 cut(s)	208, 230, 262, 324, 1012
AgsI	TTSAA	4 cut(s)	127, 343, 528, 716
AhlI	ACTAGT	1 cut(s)	907
AjnI	CCWGG	3 cut(s)	223, 517, 655
AjuI	GAANNNNNNNTTGG	2 cut(s)	849, 881
AluBI	AGCT	6 cut(s)	7, 133, 407, 535, 620, 674
AluI	AGCT	6 cut(s)	7, 133, 407, 535, 620, 674
Alw26I	GTCTC	2 cut(s)	172, 896
AlwI	GGATC	3 cut(s)	309, 322, 417
AlwNI	CAGNNNCTG	2 cut(s)	410, 518
AoxI	GGCC	4 cut(s)	52, 199, 653, 836
ApeKI	GCWGC	1 cut(s)	272
ApoI	RAATTY	1 cut(s)	107
AspLEI	GCGC	1 cut(s)	604
AspS9I	GGNCC	3 cut(s)	52, 515, 808
AsuHPI	GGTGA	3 cut(s)	487, 866, 932
AsuII	TTCGAA	1 cut(s)	111
AvaII	GGWCC	2 cut(s)	515, 808
BaeI	ACNNNNGTAYC	2 cut(s)	434, 467
BalI	TGGCCA	1 cut(s)	201
BamHI	GGATCC	1 cut(s)	314
BanII	GRGCYC	1 cut(s)	270
BbvCI	CCTCAGC	1 cut(s)	162
BbvI	GCAGC	1 cut(s)	259
BccI	CCATC	1 cut(s)	1047
BceAI	ACGGC	1 cut(s)	303
BciT130I	CCWGG	3 cut(s)	225, 519, 657
BcoDI	GTCTC	2 cut(s)	172, 896
BcuI	ACTAGT	1 cut(s)	907
BfaI	CTAG	1 cut(s)	908
BisI	GCNGC	2 cut(s)	273, 931
BlsI	GCNGC	2 cut(s)	274, 932
Bme1390I	CCNGG	3 cut(s)	225, 519, 657
Bme18I	GGWCC	2 cut(s)	515, 808
BmgT120I	GGNCC	3 cut(s)	52, 515, 808
BmiI	GGNNCC	3 cut(s)	53, 267, 316
BmrFI	CCNGG	3 cut(s)	225, 519, 657
BmsI	GCATC	3 cut(s)	727, 761, 883
Bpu10I	CCTNAGC	1 cut(s)	162
Bpu14I	TTCGAA	1 cut(s)	111
BpuEI	CTTGAG	1 cut(s)	106
Bsa29I	ATCGAT	1 cut(s)	458
BsaBI	GATNNNNATC	1 cut(s)	365
BsaHI	GRCGYC	2 cut(s)	388, 401
BsaI	GGTCTC	1 cut(s)	172
BsaJI	CCNNGG	2 cut(s)	656, 865
Bsc4I	CCNNNNNNNGG	5 cut(s)	208, 230, 262, 324, 1012
Bse1I	ACTGG	1 cut(s)	202
Bse3DI	GCAATG	1 cut(s)	297
Bse8I	GATNNNNATC	1 cut(s)	365
BseBI	CCWGG	3 cut(s)	225, 519, 657
BseCI	ATCGAT	1 cut(s)	458
BseDI	CCNNGG	2 cut(s)	656, 865
BseGI	GGATG	3 cut(s)	439, 654, 1021
BseJI	GATNNNNATC	1 cut(s)	365
BseLI	CCNNNNNNNGG	5 cut(s)	208, 230, 262, 324, 1012
BseMI	GCAATG	1 cut(s)	297
BseMII	CTCAG	2 cut(s)	153, 342
BseNI	ACTGG	1 cut(s)	202
BseXI	GCAGC	1 cut(s)	259
Bsh1236I	CGCG	1 cut(s)	17
Bsh1285I	CGRYCG	1 cut(s)	363
BshFI	GGCC	4 cut(s)	54, 201, 655, 838
BshVI	ATCGAT	1 cut(s)	458
BsiEI	CGRYCG	1 cut(s)	363
BsiSI	CCGG	2 cut(s)	391, 468
BslFI	GGGAC	1 cut(s)	674
BslI	CCNNNNNNNGG	5 cut(s)	208, 230, 262, 324, 1012
BsmAI	GTCTC	2 cut(s)	172, 896
BsmFI	GGGAC	1 cut(s)	674
BsnI	GGCC	4 cut(s)	54, 201, 655, 838
Bso31I	GGTCTC	1 cut(s)	172
Bsp119I	TTCGAA	1 cut(s)	111
Bsp1286I	GDGCHC	1 cut(s)	270
Bsp143I	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
Bsp68I	TCGCGA	1 cut(s)	17
BspACI	CCGC	5 cut(s)	270, 309, 325, 764, 930
BspANI	GGCC	4 cut(s)	54, 201, 655, 838
BspCNI	CTCAG	2 cut(s)	154, 343
BspDI	ATCGAT	1 cut(s)	458
BspFNI	CGCG	1 cut(s)	17
BspLI	GGNNCC	3 cut(s)	53, 267, 316
BspPI	GGATC	3 cut(s)	309, 322, 417
BspT104I	TTCGAA	1 cut(s)	111
BspTNI	GGTCTC	1 cut(s)	172
BsrDI	GCAATG	1 cut(s)	297
BsrI	ACTGG	1 cut(s)	202
BssECI	CCNNGG	2 cut(s)	656, 865
BssMI	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
BssNI	GRCGYC	2 cut(s)	388, 401
BssT1I	CCWWGG	1 cut(s)	865
Bst2UI	CCWGG	3 cut(s)	225, 519, 657
Bst4CI	ACNGT	2 cut(s)	211, 301
Bst6I	CTCTTC	2 cut(s)	233, 986
BstACI	GRCGYC	2 cut(s)	388, 401
BstBI	TTCGAA	1 cut(s)	111
BstC8I	GCNNGC	5 cut(s)	56, 60, 252, 270, 307
BstDEI	CTNAG	3 cut(s)	162, 174, 351
BstF5I	GGATG	3 cut(s)	439, 654, 1021
BstFNI	CGCG	1 cut(s)	17
BstHHI	GCGC	1 cut(s)	604
BstKTI	GATC	6 cut(s)	97, 317, 363, 425, 462, 925
BstMAI	GTCTC	2 cut(s)	172, 896
BstMBI	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
BstMCI	CGRYCG	1 cut(s)	363
BstMWI	GCNNNNNNNGC	1 cut(s)	772
BstNI	CCWGG	3 cut(s)	225, 519, 657
BstNSI	RCATGY	1 cut(s)	770
BstSCI	CCNGG	3 cut(s)	223, 517, 655
BstUI	CGCG	1 cut(s)	17
BstV1I	GCAGC	1 cut(s)	259
BstX2I	RGATCY	1 cut(s)	314
BstYI	RGATCY	1 cut(s)	314
Bsu15I	ATCGAT	1 cut(s)	458
BsuRI	GGCC	4 cut(s)	54, 201, 655, 838
BsuTUI	ATCGAT	1 cut(s)	458
BtsCI	GGATG	3 cut(s)	439, 654, 1021
BtsI	GCAGTG	1 cut(s)	462
BtsIMutI	CAGTG	1 cut(s)	462
BtuMI	TCGCGA	1 cut(s)	17
Cac8I	GCNNGC	5 cut(s)	56, 60, 252, 270, 307
CaiI	CAGNNNCTG	2 cut(s)	410, 518
CfoI	GCGC	1 cut(s)	604
Cfr13I	GGNCC	3 cut(s)	52, 515, 808
ClaI	ATCGAT	1 cut(s)	458
CseI	GACGC	1 cut(s)	396
Csp6I	GTAC	1 cut(s)	443
CviAII	CATG	4 cut(s)	767, 788, 805, 812
CviQI	GTAC	1 cut(s)	443
DdeI	CTNAG	3 cut(s)	162, 174, 351
DpnI	GATC	6 cut(s)	96, 316, 362, 424, 461, 924
DpnII	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
DraI	TTTAAA	1 cut(s)	976
EaeI	YGGCCR	1 cut(s)	199
Eam1104I	CTCTTC	2 cut(s)	233, 986
EarI	CTCTTC	2 cut(s)	233, 986
EciI	GGCGGA	2 cut(s)	324, 340
Eco130I	CCWWGG	1 cut(s)	865
Eco147I	AGGCCT	2 cut(s)	655, 838
Eco24I	GRGCYC	1 cut(s)	270
Eco31I	GGTCTC	1 cut(s)	172
Eco47I	GGWCC	2 cut(s)	515, 808
Eco57I	CTGAAG	2 cut(s)	467, 953
EcoO109I	RGGNCCY	2 cut(s)	52, 515
EcoRII	CCWGG	3 cut(s)	223, 517, 655
EcoT14I	CCWWGG	1 cut(s)	865
EcoT22I	ATGCAT	3 cut(s)	742, 754, 793
EcoT38I	GRGCYC	1 cut(s)	270
ErhI	CCWWGG	1 cut(s)	865
FaeI	CATG	4 cut(s)	770, 791, 808, 815
FalI	AAGNNNNNCTT	2 cut(s)	849, 881
FaqI	GGGAC	1 cut(s)	674
FatI	CATG	4 cut(s)	766, 787, 804, 811
FauI	CCCGC	1 cut(s)	277
FblI	GTMKAC	1 cut(s)	206
Fnu4HI	GCNGC	2 cut(s)	273, 931
FokI	GGATG	3 cut(s)	446, 661, 1008
FriOI	GRGCYC	1 cut(s)	270
Fsp4HI	GCNGC	2 cut(s)	273, 931
FspBI	CTAG	1 cut(s)	908
GlaI	GCGC	1 cut(s)	603
GluI	GCNGC	2 cut(s)	273, 931
HaeIII	GGCC	4 cut(s)	54, 201, 655, 838
HapII	CCGG	2 cut(s)	391, 468
HgaI	GACGC	1 cut(s)	396
HhaI	GCGC	1 cut(s)	604
Hin1I	GRCGYC	2 cut(s)	388, 401
Hin1II	CATG	4 cut(s)	770, 791, 808, 815
Hin6I	GCGC	1 cut(s)	602
HinP1I	GCGC	1 cut(s)	602
HinfI	GANTC	6 cut(s)	137, 353, 428, 455, 485, 760
HpaII	CCGG	2 cut(s)	391, 468
HphI	GGTGA	3 cut(s)	487, 866, 932
Hpy166II	GTNNAC	2 cut(s)	207, 285
Hpy188I	TCNGA	8 cut(s)	99, 120, 319, 352, 427, 433, 946, 1002
Hpy188III	TCNNGA	4 cut(s)	16, 169, 420, 508
Hpy8I	GTNNAC	2 cut(s)	207, 285
Hpy99I	CGWCG	1 cut(s)	22
HpyAV	CCTTC	7 cut(s)	73, 324, 377, 535, 597, 710, 977
HpyCH4III	ACNGT	2 cut(s)	211, 301
HpyCH4IV	ACGT	1 cut(s)	401
HpyCH4V	TGCA	5 cut(s)	305, 740, 752, 791, 1062
HpyF10VI	GCNNNNNNNGC	1 cut(s)	772
HpyF3I	CTNAG	3 cut(s)	162, 174, 351
HpySE526I	ACGT	1 cut(s)	401
Hsp92I	GRCGYC	2 cut(s)	388, 401
Hsp92II	CATG	4 cut(s)	770, 791, 808, 815
HspAI	GCGC	1 cut(s)	602
Kzo9I	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
LmnI	GCTCC	1 cut(s)	265
Lsp1109I	GCAGC	1 cut(s)	259
LweI	GCATC	3 cut(s)	727, 761, 883
MaeI	CTAG	1 cut(s)	908
MaeII	ACGT	1 cut(s)	401
MaeIII	GTNAC	6 cut(s)	34, 70, 417, 667, 800, 1017
MalI	GATC	6 cut(s)	96, 316, 362, 424, 461, 924
MboI	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
MboII	GAAGA	4 cut(s)	139, 250, 869, 1003
MfeI	CAATTG	1 cut(s)	528
MflI	RGATCY	1 cut(s)	314
MhlI	GDGCHC	1 cut(s)	270
MlsI	TGGCCA	1 cut(s)	201
MluCI	AATT	3 cut(s)	107, 528, 830
MluNI	TGGCCA	1 cut(s)	201
MlyI	GAGTC	1 cut(s)	362
MmeI	TCCRAC	1 cut(s)	432
Mox20I	TGGCCA	1 cut(s)	201
Mph1103I	ATGCAT	3 cut(s)	742, 754, 793
MscI	TGGCCA	1 cut(s)	201
MseI	TTAA	3 cut(s)	795, 833, 975
MslI	CAYNNNNRTG	2 cut(s)	290, 846
Msp20I	TGGCCA	1 cut(s)	201
MspA1I	CMGCKG	1 cut(s)	272
MspI	CCGG	2 cut(s)	391, 468
MspR9I	CCNGG	3 cut(s)	225, 519, 657
MunI	CAATTG	1 cut(s)	528
MvaI	CCWGG	3 cut(s)	225, 519, 657
MvnI	CGCG	1 cut(s)	17
MwoI	GCNNNNNNNGC	1 cut(s)	772
NdeII	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
NlaIII	CATG	4 cut(s)	770, 791, 808, 815
NlaIV	GGNNCC	3 cut(s)	53, 267, 316
NmuCI	GTSAC	2 cut(s)	417, 667
NruI	TCGCGA	1 cut(s)	17
NsiI	ATGCAT	3 cut(s)	742, 754, 793
NspI	RCATGY	1 cut(s)	770
NspV	TTCGAA	1 cut(s)	111
PceI	AGGCCT	2 cut(s)	655, 838
PfeI	GAWTC	5 cut(s)	137, 428, 455, 485, 760
PflFI	GACNNNGTC	1 cut(s)	665
PkrI	GCNGC	2 cut(s)	274, 932
Ple19I	CGATCG	1 cut(s)	363
PleI	GAGTC	1 cut(s)	361
PpsI	GAGTC	1 cut(s)	361
PpuMI	RGGWCCY	1 cut(s)	515
Psp5II	RGGWCCY	1 cut(s)	515
Psp6I	CCWGG	3 cut(s)	223, 517, 655
PspGI	CCWGG	3 cut(s)	223, 517, 655
PspN4I	GGNNCC	3 cut(s)	53, 267, 316
PspPI	GGNCC	3 cut(s)	52, 515, 808
PspPPI	RGGWCCY	1 cut(s)	515
PstNI	CAGNNNCTG	2 cut(s)	410, 518
PsuI	RGATCY	1 cut(s)	314
PsyI	GACNNNGTC	1 cut(s)	665
PvuI	CGATCG	1 cut(s)	363
RruI	TCGCGA	1 cut(s)	17
RsaI	GTAC	1 cut(s)	444
RsaNI	GTAC	1 cut(s)	443
RseI	CAYNNNNRTG	2 cut(s)	290, 846
SaqAI	TTAA	3 cut(s)	795, 833, 975
SatI	GCNGC	2 cut(s)	273, 931
Sau3AI	GATC	6 cut(s)	94, 314, 360, 422, 459, 922
Sau96I	GGNCC	3 cut(s)	52, 515, 808
SchI	GAGTC	1 cut(s)	362
ScrFI	CCNGG	3 cut(s)	225, 519, 657
SduI	GDGCHC	1 cut(s)	270
SfaNI	GCATC	3 cut(s)	727, 761, 883
SfuI	TTCGAA	1 cut(s)	111
SinI	GGWCC	2 cut(s)	515, 808
SmiMI	CAYNNNNRTG	2 cut(s)	290, 846
SmlI	CTYRAG	1 cut(s)	85
SmoI	CTYRAG	1 cut(s)	85
SpeI	ACTAGT	1 cut(s)	907
Sse9I	AATT	3 cut(s)	107, 528, 830
SseBI	AGGCCT	2 cut(s)	655, 838
SsiI	CCGC	5 cut(s)	270, 309, 325, 764, 930
SspMI	CTAG	1 cut(s)	908
StuI	AGGCCT	2 cut(s)	655, 838
StyD4I	CCNGG	3 cut(s)	223, 517, 655
StyI	CCWWGG	1 cut(s)	865
TaaI	ACNGT	2 cut(s)	211, 301
TaiI	ACGT	1 cut(s)	404
TaqI	TCGA	7 cut(s)	104, 111, 170, 236, 363, 381, 458
TasI	AATT	3 cut(s)	107, 528, 830
TauI	GCSGC	1 cut(s)	933
TfiI	GAWTC	5 cut(s)	137, 428, 455, 485, 760
Tru1I	TTAA	3 cut(s)	795, 833, 975
Tru9I	TTAA	3 cut(s)	795, 833, 975
TscAI	CASTG	1 cut(s)	469
TseFI	GTSAC	2 cut(s)	417, 667
TseI	GCWGC	1 cut(s)	272
Tsp45I	GTSAC	2 cut(s)	417, 667
TspDTI	ATGAA	5 cut(s)	17, 554, 598, 843, 963
TspGWI	ACGGA	2 cut(s)	53, 278
TspRI	CASTG	1 cut(s)	469
Tth111I	GACNNNGTC	1 cut(s)	665
VpaK11BI	GGWCC	2 cut(s)	515, 808
XapI	RAATTY	1 cut(s)	107
XceI	RCATGY	1 cut(s)	770
XmiI	GTMKAC	1 cut(s)	206
XspI	CTAG	1 cut(s)	908
ZraI	GACGTC	1 cut(s)	402
Zsp2I	ATGCAT	3 cut(s)	742, 754, 793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.