Rmu_sc0001365.1_g000022

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001365.1
OG0002284
Physical Location & Seq
Reverse (-)
76010 .. 78434
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001365.1_g000022.1.cds

Sequence Viewer

Length: 1065 bp
atggggatagaagcgaagattttggaagcggccctgcactctaatcaagcaaatttaaagaagaagaaaatagatggtaaagagaaagatgtggcggatgagggtgagcaagaaataaggagattgagctggttatggaagttccggcaggcaagtctcaggaggagactacatttgtcattggagaaccggatgtggcagacaaaggtgaagaggaaagttggtgcgtcggccattccccttgaccacacgaaaagagtaaagatttctaaaaagaatgtggcggttggcagtagtcaaggttccgagggaggcgggttttgtttccaacaatcctgttcttgggttggtgtagagtgcacccactggtcaatagggtttctgtacaagggttcattggaggagcatcagttgaagaaagagtttctacaaacgcaacatgacttaatgatagagagagagagaaaaaaaacaactaaggggaacattgccaagctcgatggtgcctttcttgacgttaaggaaaagttcaagcgtgcggttgacaaaactatcgccgataccaccaccatctctaacctgaactttgagttggcggctttgaaaacagagaaagctgatagggaggatactatctcccaactgcaggaggcagttatcgccaaagaaaaggagttggcggacttccagtctgcaaggcgagaggaggtgacgaagatggtagaagatgagaaaacaagagttggcctggaagcagtggaggccttcaaaaagttcgcggagttcacagagcttttgacaagacagtttgatatcgagcttcgccaagtcccttggcaggcgaagaaccgacccagtcgacctgacccgacgctagcggcgccgtcgcgcgtcctccggccacgacccggcgcttcctactttcacctagagccgcccagacgctttgtggcgtcgccttgcccagccgccggccccagtcttggatcgaagacacccagccaccgatcggctgacccggacggatttccgacttccggcgacttgccgacgaatttattctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

354

Amino Acids

40.01

Weight (kDa)

9.49

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 503, 880
AccI GTMKAC 1 cut(s) 859
AccII CGCG 3 cut(s) 779, 889, 891
AclWI GGATC 1 cut(s) 994
AcoI YGGCCR 2 cut(s) 231, 899
AcsI RAATTY 2 cut(s) 52, 1054
AcyI GRCGYC 2 cut(s) 881, 953
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 8 cut(s) 342, 646, 838, 908, 971, 983, 1009, 1037
AgsI TTSAA 4 cut(s) 415, 532, 604, 769
AhdI GACNNNNNGTC 1 cut(s) 688
AjnI CCWGG 1 cut(s) 747
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 5 cut(s) 129, 496, 617, 793, 820
AluI AGCT 5 cut(s) 129, 496, 617, 793, 820
Alw21I GWGCWC 1 cut(s) 362
Alw26I GTCTC 2 cut(s) 160, 161
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 1 cut(s) 994
AoxI GGCC 6 cut(s) 30, 231, 745, 762, 899, 973
ApaLI GTGCAC 1 cut(s) 358
ApoI RAATTY 2 cut(s) 52, 1054
Asp700I GAANNNNTTC 1 cut(s) 1058
AspLEI GCGC 3 cut(s) 883, 891, 914
AspS9I GGNCC 2 cut(s) 31, 974
AsuC2I CCSGG 2 cut(s) 909, 1019
AsuHPI GGTGA 4 cut(s) 116, 220, 721, 917
AsuNHI GCTAGC 1 cut(s) 874
BaeGI GKGCMC 1 cut(s) 362
BanI GGYRCC 2 cut(s) 503, 880
BbsI GAAGAC 1 cut(s) 998
Bbv12I GWGCWC 1 cut(s) 362
BccI CCATC 4 cut(s) 68, 494, 578, 712
BceAI ACGGC 1 cut(s) 868
BciT130I CCWGG 1 cut(s) 749
BciVI GTATCC 1 cut(s) 622
BcnI CCSGG 2 cut(s) 909, 1019
BcoDI GTCTC 2 cut(s) 160, 161
BfaI CTAG 3 cut(s) 875, 929, 1063
BfmI CTRYAG 1 cut(s) 644
BfoI RGCGCY 2 cut(s) 884, 915
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 5 cut(s) 30, 597, 879, 935, 969
BlsI GCNGC 5 cut(s) 31, 598, 880, 936, 970
Bme1390I CCNGG 3 cut(s) 749, 909, 1019
BmeRI GACNNNNNGTC 1 cut(s) 688
BmgT120I GGNCC 2 cut(s) 31, 974
BmiI GGNNCC 4 cut(s) 304, 505, 882, 976
BmrFI CCNGG 3 cut(s) 749, 909, 1019
BmrI ACTGGG 2 cut(s) 849, 972
BmsI GCATC 1 cut(s) 415
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 2 cut(s) 849, 972
BpiI GAAGAC 1 cut(s) 998
BpuMI CCSGG 2 cut(s) 909, 1019
BsaHI GRCGYC 2 cut(s) 881, 953
BsaJI CCNNGG 2 cut(s) 306, 833
BsaWI WCCGGW 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 8 cut(s) 342, 646, 838, 908, 971, 983, 1009, 1037
Bse118I RCCGGY 1 cut(s) 971
Bse1I ACTGG 4 cut(s) 371, 688, 855, 978
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 749
BseDI CCNNGG 2 cut(s) 306, 833
BseGI GGATG 2 cut(s) 103, 198
BseLI CCNNNNNNNGG 8 cut(s) 342, 646, 838, 908, 971, 983, 1009, 1037
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 172
BseNI ACTGG 4 cut(s) 371, 688, 855, 978
BseRI GAGGAG 3 cut(s) 178, 416, 719
BseSI GKGCMC 1 cut(s) 362
BseYI CCCAGC 2 cut(s) 964, 998
BsgI GTGCAG 1 cut(s) 20
Bsh1236I CGCG 3 cut(s) 779, 889, 891
Bsh1285I CGRYCG 1 cut(s) 1010
BshFI GGCC 6 cut(s) 32, 233, 747, 764, 901, 975
BshNI GGYRCC 2 cut(s) 503, 880
BsiEI CGRYCG 1 cut(s) 1010
BsiHKAI GWGCWC 1 cut(s) 362
BsiSI CCGG 7 cut(s) 145, 190, 898, 909, 972, 1019, 1038
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 8 cut(s) 342, 646, 838, 908, 971, 983, 1009, 1037
BsmAI GTCTC 2 cut(s) 160, 161
BsmFI GGGAC 1 cut(s) 815
BsnI GGCC 6 cut(s) 32, 233, 747, 764, 901, 975
Bsp1286I GDGCHC 1 cut(s) 362
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 2 cut(s) 986, 1007
BspANI GGCC 6 cut(s) 32, 233, 747, 764, 901, 975
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 3 cut(s) 779, 889, 891
BspLI GGNNCC 4 cut(s) 304, 505, 882, 976
BspMAI CTGCAG 1 cut(s) 648
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 1 cut(s) 994
BspT107I GGYRCC 2 cut(s) 503, 880
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 971
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 4 cut(s) 371, 688, 855, 978
BssAI RCCGGY 1 cut(s) 971
BssECI CCNNGG 2 cut(s) 306, 833
BssMI GATC 2 cut(s) 986, 1007
BssNI GRCGYC 2 cut(s) 881, 953
BssT1I CCWWGG 1 cut(s) 833
Bst2UI CCWGG 1 cut(s) 749
Bst4CI ACNGT 1 cut(s) 807
Bst6I CTCTTC 1 cut(s) 206
BstACI GRCGYC 2 cut(s) 881, 953
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 5 cut(s) 150, 537, 840, 876, 973
BstDEI CTNAG 2 cut(s) 158, 477
BstF5I GGATG 2 cut(s) 103, 198
BstFNI CGCG 3 cut(s) 779, 889, 891
BstH2I RGCGCY 2 cut(s) 884, 915
BstHHI GCGC 3 cut(s) 883, 891, 914
BstKTI GATC 2 cut(s) 989, 1010
BstMAI GTCTC 2 cut(s) 160, 161
BstMBI GATC 2 cut(s) 986, 1007
BstMCI CGRYCG 1 cut(s) 1010
BstMWI GCNNNNNNNGC 3 cut(s) 659, 761, 880
BstNI CCWGG 1 cut(s) 749
BstSCI CCNGG 3 cut(s) 747, 907, 1017
BstSFI CTRYAG 1 cut(s) 644
BstSLI GKGCMC 1 cut(s) 362
BstUI CGCG 3 cut(s) 779, 889, 891
BstV2I GAAGAC 1 cut(s) 998
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 6 cut(s) 32, 233, 747, 764, 901, 975
BtsCI GGATG 2 cut(s) 103, 198
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 2 cut(s) 364, 762
Cac8I GCNNGC 5 cut(s) 150, 537, 840, 876, 973
CfoI GCGC 3 cut(s) 883, 891, 914
Cfr10I RCCGGY 1 cut(s) 971
Cfr13I GGNCC 2 cut(s) 31, 974
CseI GACGC 5 cut(s) 216, 880, 880, 942, 951
Csp6I GTAC 1 cut(s) 385
CviAII CATG 1 cut(s) 440
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 158, 477
DinI GGCGCC 1 cut(s) 882
DpnI GATC 2 cut(s) 988, 1009
DpnII GATC 2 cut(s) 986, 1007
DraI TTTAAA 1 cut(s) 57
DriI GACNNNNNGTC 1 cut(s) 688
EaeI YGGCCR 2 cut(s) 231, 899
Eam1104I CTCTTC 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 688
EarI CTCTTC 1 cut(s) 206
EciI GGCGGA 2 cut(s) 110, 695
Eco130I CCWWGG 1 cut(s) 833
Eco147I AGGCCT 1 cut(s) 764
Eco32I GATATC 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 747
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 833
EgeI GGCGCC 1 cut(s) 882
EheI GGCGCC 1 cut(s) 882
ErhI CCWWGG 1 cut(s) 833
FaeI CATG 1 cut(s) 443
FaiI YATR 2 cut(s) 136, 441
FaqI GGGAC 1 cut(s) 815
FatI CATG 1 cut(s) 439
FauI CCCGC 1 cut(s) 308
FblI GTMKAC 1 cut(s) 859
Fnu4HI GCNGC 5 cut(s) 30, 597, 879, 935, 969
FokI GGATG 2 cut(s) 110, 205
Fsp4HI GCNGC 5 cut(s) 30, 597, 879, 935, 969
FspBI CTAG 3 cut(s) 875, 929, 1063
GlaI GCGC 3 cut(s) 882, 890, 913
GluI GCNGC 5 cut(s) 30, 597, 879, 935, 969
GsaI CCCAGC 2 cut(s) 968, 1002
HaeII RGCGCY 2 cut(s) 884, 915
HaeIII GGCC 6 cut(s) 32, 233, 747, 764, 901, 975
HapII CCGG 7 cut(s) 145, 190, 898, 909, 972, 1019, 1038
HgaI GACGC 5 cut(s) 216, 880, 880, 942, 951
HhaI GCGC 3 cut(s) 883, 891, 914
Hin1I GRCGYC 2 cut(s) 881, 953
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 3 cut(s) 881, 889, 912
HinP1I GCGC 3 cut(s) 881, 889, 912
HincII GTYRAC 2 cut(s) 544, 860
HindII GTYRAC 2 cut(s) 544, 860
HpaII CCGG 7 cut(s) 145, 190, 898, 909, 972, 1019, 1038
HphI GGTGA 4 cut(s) 116, 220, 721, 917
Hpy166II GTNNAC 4 cut(s) 360, 544, 786, 860
Hpy188I TCNGA 2 cut(s) 307, 1032
Hpy188III TCNNGA 2 cut(s) 160, 512
Hpy8I GTNNAC 4 cut(s) 360, 544, 786, 860
Hpy99I CGWCG 5 cut(s) 232, 874, 889, 958, 1054
HpyAV CCTTC 1 cut(s) 775
HpyCH4III ACNGT 1 cut(s) 807
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 4 cut(s) 37, 360, 646, 695
HpyF10VI GCNNNNNNNGC 3 cut(s) 659, 761, 880
HpyF3I CTNAG 2 cut(s) 158, 477
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 2 cut(s) 881, 953
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 3 cut(s) 881, 889, 912
KasI GGCGCC 1 cut(s) 880
KroI GCCGGC 1 cut(s) 971
KroNI GCCGGC 1 cut(s) 973
Kzo9I GATC 2 cut(s) 986, 1007
LmnI GCTCC 1 cut(s) 403
LweI GCATC 1 cut(s) 415
MaeI CTAG 3 cut(s) 875, 929, 1063
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 709
MalI GATC 2 cut(s) 988, 1009
MboI GATC 2 cut(s) 986, 1007
MboII GAAGA 9 cut(s) 28, 73, 76, 223, 427, 727, 737, 856, 1003
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 2 cut(s) 52, 1054
Mly113I GGCGCC 1 cut(s) 881
MmeI TCCRAC 2 cut(s) 352, 1055
MroNI GCCGGC 1 cut(s) 971
MroXI GAANNNNTTC 1 cut(s) 1058
MseI TTAA 3 cut(s) 56, 446, 519
MspI CCGG 7 cut(s) 145, 190, 898, 909, 972, 1019, 1038
MspR9I CCNGG 3 cut(s) 749, 909, 1019
MvaI CCWGG 1 cut(s) 749
MvnI CGCG 3 cut(s) 779, 889, 891
MwoI GCNNNNNNNGC 3 cut(s) 659, 761, 880
NaeI GCCGGC 1 cut(s) 973
NarI GGCGCC 1 cut(s) 881
NciI CCSGG 2 cut(s) 909, 1019
NdeII GATC 2 cut(s) 986, 1007
NgoMIV GCCGGC 1 cut(s) 971
NheI GCTAGC 1 cut(s) 874
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 4 cut(s) 304, 505, 882, 976
NmuCI GTSAC 1 cut(s) 709
PceI AGGCCT 1 cut(s) 764
PdiI GCCGGC 1 cut(s) 973
PdmI GAANNNNTTC 1 cut(s) 1058
PflFI GACNNNGTC 1 cut(s) 855
PkrI GCNGC 5 cut(s) 31, 598, 880, 936, 970
Ple19I CGATCG 1 cut(s) 1010
PluTI GGCGCC 1 cut(s) 884
Psp6I CCWGG 1 cut(s) 747
PspFI CCCAGC 2 cut(s) 964, 998
PspGI CCWGG 1 cut(s) 747
PspN4I GGNNCC 4 cut(s) 304, 505, 882, 976
PspPI GGNCC 2 cut(s) 31, 974
PsrI GAACNNNNNNTAC 2 cut(s) 286, 318
PstI CTGCAG 1 cut(s) 648
PsyI GACNNNGTC 1 cut(s) 855
PvuI CGATCG 1 cut(s) 1010
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SalI GTCGAC 1 cut(s) 858
SaqAI TTAA 3 cut(s) 56, 446, 519
SatI GCNGC 5 cut(s) 30, 597, 879, 935, 969
Sau3AI GATC 2 cut(s) 986, 1007
Sau96I GGNCC 2 cut(s) 31, 974
ScrFI CCNGG 3 cut(s) 749, 909, 1019
SduI GDGCHC 1 cut(s) 362
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 1 cut(s) 644
SfoI GGCGCC 1 cut(s) 882
Sse9I AATT 2 cut(s) 52, 1054
SseBI AGGCCT 1 cut(s) 764
SspDI GGCGCC 1 cut(s) 880
SspMI CTAG 3 cut(s) 875, 929, 1063
StuI AGGCCT 1 cut(s) 764
StyD4I CCNGG 3 cut(s) 747, 907, 1017
StyI CCWWGG 1 cut(s) 833
TaaI ACNGT 1 cut(s) 807
TaiI ACGT 1 cut(s) 519
TaqI TCGA 4 cut(s) 498, 816, 859, 989
TasI AATT 2 cut(s) 52, 1054
TatI WGTACW 1 cut(s) 384
TauI GCSGC 5 cut(s) 32, 599, 881, 937, 971
Tru1I TTAA 3 cut(s) 56, 446, 519
Tru9I TTAA 3 cut(s) 56, 446, 519
TscAI CASTG 2 cut(s) 371, 762
TseFI GTSAC 1 cut(s) 709
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 1038
TspRI CASTG 2 cut(s) 371, 762
Tth111I GACNNNGTC 1 cut(s) 855
VneI GTGCAC 1 cut(s) 358
XapI RAATTY 2 cut(s) 52, 1054
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XmiI GTMKAC 1 cut(s) 859
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 3 cut(s) 875, 929, 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.