Rmu_ssc0000008.1_g000032

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000008.1
OG0002218
Physical Location & Seq
Reverse (-)
105711 .. 108196
2486 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
atggcggtcgctaccttttccaatttctccactttcaccactcgcaacccctctctctcttcttcttcaccaaatcaccactctttcccgcgcaccacaaacttcactttcactactaccctcaccaaacctcccacctccctctctctcgccacgtcaccgcgcctcccccgctcctctttcgccacgtgtttctcctccggcggcccaccttcctccggcggaggcggcgggggaggagatcacggaaatggaggcggtgacggagagggaggggaggacagagagaggaacaaaaaggaggcggcgatggcgattgcggaggcgggaaggacggcggagagtctgccgaaggacttggcggcggcggtggaggcgggtcgggtccccgggttgattatgaagaggttctttgagctggagaagtcggtgatcttcaggtggctgcttcagtttgatggattcaaggagcggttgctggccgacgacttgttcttgaccaaagttggcatcgagtgcggtgttggaattttcaccaagactgctgcagagttggagcggcgcagagaaaaatttaccaaggagctggattttgtgtttgcggatgtggtgatggccattatagctgattttatgcttgtctggcttcctgctcctactgtttcacttcgaaaacctcttgttgtcagtgctgggcgtgttgctaagttcttctatggctgtcctgacaatgcatttcaggtagctttgtctggaacatcttactcagtcttacagagaataggcgcaatcttgagaaatggagcaaaactattcgtggttgggactggtgcatctctgattggtactggtgtaacaaatgcattgattgctgcaagaaaagctttggacaaatcctttcttggtgaagcagaagatgttcccatattatcaactagtgcagcctatggggtctatatggcagtttctagcaatctaaggtaccaagttctcgctggaataatcgaacaacgtattctagaacccttgctacaccaacacaagcttgcactgagtgcaatttgctttgctgttcgaactggaaacacatttttggggtccttgatgtgggttgattatgcacgctggattggaattcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

380

Amino Acids

40.75

Weight (kDa)

9.24

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 157 - 334 5.9e-66 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 981
AccB1I GGYRCC 1 cut(s) 981
AccBSI CCGCTC 3 cut(s) 174, 472, 559
AccII CGCG 2 cut(s) 91, 163
AcoI YGGCCR 2 cut(s) 480, 615
AcsI RAATTY 3 cut(s) 528, 572, 1134
AcuI CTGAAG 2 cut(s) 421, 434
AcvI CACGTG 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 1055
AfaI GTAC 2 cut(s) 847, 983
AfiI CCNNNNNNNGG 2 cut(s) 218, 1107
AflIII ACRYGT 1 cut(s) 188
AgsI TTSAA 2 cut(s) 466, 1139
AhlI ACTAGT 1 cut(s) 935
AjiI CACGTC 1 cut(s) 156
AluBI AGCT 6 cut(s) 418, 586, 626, 746, 884, 1045
AluI AGCT 6 cut(s) 418, 586, 626, 746, 884, 1045
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 3 cut(s) 205, 480, 615
ApeKI GCWGC 4 cut(s) 445, 545, 872, 941
ApoI RAATTY 3 cut(s) 528, 572, 1134
Asp700I GAANNNNTTC 2 cut(s) 407, 918
Asp718I GGTACC 1 cut(s) 981
AspLEI GCGC 4 cut(s) 93, 165, 564, 788
AspS9I GGNCC 3 cut(s) 206, 385, 1098
AsuC2I CCSGG 2 cut(s) 390, 391
AsuII TTCGAA 2 cut(s) 670, 1075
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 2 cut(s) 385, 1098
BaeI ACNNNNGTAYC 2 cut(s) 837, 870
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 981
BbrPI CACGTG 1 cut(s) 189
BbvI GCAGC 4 cut(s) 432, 532, 859, 953
BccI CCATC 3 cut(s) 304, 452, 607
BceAI ACGGC 1 cut(s) 351
BcnI CCSGG 2 cut(s) 390, 391
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 3 cut(s) 936, 969, 1019
BfmI CTRYAG 1 cut(s) 546
Bme1390I CCNGG 2 cut(s) 390, 391
Bme18I GGWCC 2 cut(s) 385, 1098
BmeT110I CYCGRG 1 cut(s) 389
BmgBI CACGTC 1 cut(s) 156
BmgT120I GGNCC 3 cut(s) 206, 385, 1098
BmiI GGNNCC 4 cut(s) 386, 387, 983, 1099
BmrFI CCNGG 2 cut(s) 390, 391
BmsI GCATC 2 cut(s) 519, 842
BpmI CTGGAG 1 cut(s) 440
Bpu14I TTCGAA 2 cut(s) 670, 1075
BpuEI CTTGAG 1 cut(s) 814
BpuMI CCSGG 2 cut(s) 390, 391
BsaAI YACGTR 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 388, 389, 579
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 150, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 1107
Bse1I ACTGG 3 cut(s) 832, 853, 1084
BseDI CCNNGG 3 cut(s) 388, 389, 579
BseGI GGATG 1 cut(s) 610
BseLI CCNNNNNNNGG 2 cut(s) 218, 1107
BseMII CTCAG 2 cut(s) 780, 1043
BseNI ACTGG 3 cut(s) 832, 853, 1084
BseRI GAGGAG 3 cut(s) 166, 187, 252
BseXI GCAGC 4 cut(s) 432, 532, 859, 953
BseYI CCCAGC 1 cut(s) 692
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 2 cut(s) 91, 163
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 3 cut(s) 207, 482, 617
BshNI GGYRCC 1 cut(s) 981
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 3 cut(s) 201, 219, 390
BslFI GGGAC 2 cut(s) 371, 838
BslI CCNNNNNNNGG 2 cut(s) 218, 1107
BsmFI GGGAC 2 cut(s) 371, 838
BsnI GGCC 3 cut(s) 207, 482, 617
BsoBI CYCGRG 1 cut(s) 389
Bsp119I TTCGAA 2 cut(s) 670, 1075
Bsp143I GATC 2 cut(s) 241, 432
BspANI GGCC 3 cut(s) 207, 482, 617
BspCNI CTCAG 2 cut(s) 779, 1044
BspFNI CGCG 2 cut(s) 91, 163
BspLI GGNNCC 4 cut(s) 386, 387, 983, 1099
BspMAI CTGCAG 1 cut(s) 550
BspT104I TTCGAA 2 cut(s) 670, 1075
BspT107I GGYRCC 1 cut(s) 981
BsrBI CCGCTC 3 cut(s) 174, 472, 559
BsrI ACTGG 3 cut(s) 832, 853, 1084
BssECI CCNNGG 3 cut(s) 388, 389, 579
BssMI GATC 2 cut(s) 241, 432
BssT1I CCWWGG 1 cut(s) 579
Bst4CI ACNGT 1 cut(s) 661
Bst6I CTCTTC 2 cut(s) 64, 398
BstAPI GCANNNNNTGC 3 cut(s) 516, 869, 1055
BstBAI YACGTR 1 cut(s) 189
BstBI TTCGAA 2 cut(s) 670, 1075
BstC8I GCNNGC 3 cut(s) 480, 1047, 1123
BstDEI CTNAG 4 cut(s) 705, 766, 977, 1052
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 2 cut(s) 91, 163
BstHHI GCGC 4 cut(s) 93, 165, 564, 788
BstKTI GATC 2 cut(s) 244, 435
BstMBI GATC 2 cut(s) 241, 432
BstMCI CGRYCG 1 cut(s) 9
BstSCI CCNGG 2 cut(s) 388, 389
BstSFI CTRYAG 1 cut(s) 546
BstUI CGCG 2 cut(s) 91, 163
BstV1I GCAGC 4 cut(s) 432, 532, 859, 953
BstXI CCANNNNNNTGG 1 cut(s) 586
BsuRI GGCC 3 cut(s) 207, 482, 617
BtgZI GCGATG 1 cut(s) 323
BtrI CACGTC 1 cut(s) 156
BtsCI GGATG 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 694, 1049
Cac8I GCNNGC 3 cut(s) 480, 1047, 1123
CfoI GCGC 4 cut(s) 93, 165, 564, 788
Cfr13I GGNCC 3 cut(s) 206, 385, 1098
Cfr9I CCCGGG 1 cut(s) 389
Csp6I GTAC 2 cut(s) 846, 982
CviQI GTAC 2 cut(s) 846, 982
DdeI CTNAG 4 cut(s) 705, 766, 977, 1052
DpnI GATC 2 cut(s) 243, 434
DpnII GATC 2 cut(s) 241, 432
DraIII CACNNNGTG 1 cut(s) 1055
EaeI YGGCCR 2 cut(s) 480, 615
Eam1104I CTCTTC 2 cut(s) 64, 398
EarI CTCTTC 2 cut(s) 64, 398
EciI GGCGGA 2 cut(s) 237, 353
Eco130I CCWWGG 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 385, 1098
Eco57I CTGAAG 2 cut(s) 421, 434
Eco72I CACGTG 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 389
EcoO109I RGGNCCY 2 cut(s) 385, 1098
EcoRI GAATTC 1 cut(s) 1134
EcoT14I CCWWGG 1 cut(s) 579
EcoT22I ATGCAT 2 cut(s) 736, 865
ErhI CCWWGG 1 cut(s) 579
FaiI YATR 9 cut(s) 401, 623, 635, 717, 926, 948, 957, 959, 1119
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 868, 900
FaqI GGGAC 2 cut(s) 371, 838
FauI CCCGC 5 cut(s) 96, 179, 224, 319, 370
FokI GGATG 1 cut(s) 617
FspBI CTAG 3 cut(s) 936, 969, 1019
GlaI GCGC 4 cut(s) 92, 164, 563, 787
GsaI CCCAGC 1 cut(s) 696
GsuI CTGGAG 1 cut(s) 440
HaeIII GGCC 3 cut(s) 207, 482, 617
HapII CCGG 3 cut(s) 201, 219, 390
HhaI GCGC 4 cut(s) 93, 165, 564, 788
Hin6I GCGC 4 cut(s) 91, 163, 562, 786
HinP1I GCGC 4 cut(s) 91, 163, 562, 786
HindIII AAGCTT 2 cut(s) 882, 1043
HinfI GANTC 2 cut(s) 343, 462
HpaII CCGG 3 cut(s) 201, 219, 390
Hpy188I TCNGA 1 cut(s) 840
Hpy188III TCNNGA 5 cut(s) 496, 725, 753, 793, 1019
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 3 cut(s) 222, 324, 346
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4IV ACGT 3 cut(s) 155, 188, 1012
HpyCH4V TGCA 9 cut(s) 548, 734, 833, 863, 875, 941, 1049, 1058, 1121
HpyF3I CTNAG 4 cut(s) 705, 766, 977, 1052
HpySE526I ACGT 3 cut(s) 155, 188, 1012
HspAI GCGC 4 cut(s) 91, 163, 562, 786
KflI GGGWCCC 1 cut(s) 385
KpnI GGTACC 1 cut(s) 985
Kzo9I GATC 2 cut(s) 241, 432
LmnI GCTCC 6 cut(s) 179, 469, 556, 583, 658, 803
Lsp1109I GCAGC 4 cut(s) 432, 532, 859, 953
LweI GCATC 2 cut(s) 519, 842
MaeI CTAG 3 cut(s) 936, 969, 1019
MaeII ACGT 3 cut(s) 155, 188, 1012
MaeIII GTNAC 3 cut(s) 156, 260, 853
MalI GATC 2 cut(s) 243, 434
MbiI CCGCTC 3 cut(s) 174, 472, 559
MboI GATC 2 cut(s) 241, 432
MboII GAAGA 7 cut(s) 51, 54, 57, 415, 427, 703, 926
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 5 cut(s) 22, 528, 572, 1059, 1134
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 2 cut(s) 505, 534
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 2 cut(s) 736, 865
MroXI GAANNNNTTC 2 cut(s) 407, 918
MscI TGGCCA 1 cut(s) 617
MslI CAYNNNNRTG 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 617
MspI CCGG 3 cut(s) 201, 219, 390
MspR9I CCNGG 2 cut(s) 390, 391
MvnI CGCG 2 cut(s) 91, 163
NciI CCSGG 2 cut(s) 390, 391
NdeII GATC 2 cut(s) 241, 432
NlaIV GGNNCC 4 cut(s) 386, 387, 983, 1099
NmuCI GTSAC 2 cut(s) 156, 260
NsiI ATGCAT 2 cut(s) 736, 865
NspV TTCGAA 2 cut(s) 670, 1075
PdmI GAANNNNTTC 2 cut(s) 407, 918
PfeI GAWTC 1 cut(s) 462
PleI GAGTC 1 cut(s) 351
PmaCI CACGTG 1 cut(s) 189
PmlI CACGTG 1 cut(s) 189
PpsI GAGTC 1 cut(s) 351
Ppu21I YACGTR 1 cut(s) 189
PpuMI RGGWCCY 2 cut(s) 385, 1098
Psp5II RGGWCCY 2 cut(s) 385, 1098
PspCI CACGTG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 692
PspN4I GGNNCC 4 cut(s) 386, 387, 983, 1099
PspPI GGNCC 3 cut(s) 206, 385, 1098
PspPPI RGGWCCY 2 cut(s) 385, 1098
PsrI GAACNNNNNNTAC 2 cut(s) 1014, 1046
PstI CTGCAG 1 cut(s) 550
RsaI GTAC 2 cut(s) 847, 983
RsaNI GTAC 2 cut(s) 846, 982
RseI CAYNNNNRTG 1 cut(s) 249
Sau3AI GATC 2 cut(s) 241, 432
Sau96I GGNCC 3 cut(s) 206, 385, 1098
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 390, 391
SfaNI GCATC 2 cut(s) 519, 842
SfcI CTRYAG 1 cut(s) 546
SfuI TTCGAA 2 cut(s) 670, 1075
SinI GGWCC 2 cut(s) 385, 1098
SmaI CCCGGG 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 793
SmoI CTYRAG 1 cut(s) 793
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 5 cut(s) 22, 528, 572, 1059, 1134
SspMI CTAG 3 cut(s) 936, 969, 1019
StyD4I CCNGG 2 cut(s) 388, 389
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 1 cut(s) 661
TaiI ACGT 3 cut(s) 158, 191, 1015
TaqI TCGA 4 cut(s) 513, 670, 1005, 1075
TasI AATT 5 cut(s) 22, 528, 572, 1059, 1134
TauI GCSGC 6 cut(s) 207, 231, 308, 365, 368, 562
TfiI GAWTC 1 cut(s) 462
TscAI CASTG 2 cut(s) 694, 1056
TseFI GTSAC 2 cut(s) 156, 260
TseI GCWGC 4 cut(s) 445, 545, 872, 941
Tsp45I GTSAC 2 cut(s) 156, 260
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 2 cut(s) 261, 279
TspMI CCCGGG 1 cut(s) 389
TspRI CASTG 2 cut(s) 694, 1056
VpaK11BI GGWCC 2 cut(s) 385, 1098
XapI RAATTY 3 cut(s) 528, 572, 1134
XbaI TCTAGA 1 cut(s) 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 992
XmaI CCCGGG 1 cut(s) 389
XmnI GAANNNNTTC 2 cut(s) 407, 918
XspI CTAG 3 cut(s) 936, 969, 1019
Zsp2I ATGCAT 2 cut(s) 736, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.