Rroxscaffold_1G00040670

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002406
Physical Location & Seq
Reverse (-)
58436362 .. 58438066
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040670.1

Sequence Viewer

Length: 894 bp
ATGGAAGGGTGCAATCTTCTCCTTAGAATAGACAATCTAGATCTAGATAGTCAGGACGAGGAAAAAACAGAGACAACAGAGGAAGCAAATGTTCCTAGGAGGTATTACTGTTATCAGTGTCGTCAAGTGGTCAGCGTCACTGTGGAAGTCGAAATTACATGCCCTCTCTGCCATGGCGGCTGGATACAAGAATGTGATGGCGTTAGCATACTCTATCTTGCTATGCCATCAGAATCCGAGCATTCGGCAAGTGATTCTGATGGACAGGGCACCATTCTGATCCATCCTTCCAGTCAAACTGTCCTGGACAGTGTCGAGGACAGCTATAGTTCAGATCCCAACCCCAACACGTCCCCTTTTCATAGCACTGCTTCCCATGATTACGACAACTATGTCGAGTCACACCAGGCAACGGGCCATGATGATTCGAGTTTGGAACAGGGATTACTAACACTTAGCATTGATGCAAATATTGTTGCTAACCAGCCTCATCATGAGACAAGGGTTACTGCATCGGTCTGGATCTTTTCAGCCACTGGTTTAGCCTTTGAGGCTGTATCGGCATTTTTCGATCAGGTTTCCACCCCAGGGAAGCCTCAGTACTCGTTGTATGGCATGCTATTGTCCATCGTGGCTGTACTTGTTTGCATCTTGGATCTCATTCACAATGCCCTGATACAAAGACAAAGGGGACTTGCATCTGCGAGTAACATTATTTGGGGTATCTTGCCTGAAGTTTTTGGATTAGCTCTCGCATGTATTCAATGCATTAGCTCGGCACTGCAGTTTTCATTTCTCTGTAGGCATGCTGCTAATCCTGTGAAAATATCGCTTGTGCCTTTCTTATTTTTCTTCGTTTTGTTTGCTTTCAAATATAGAGAAGGAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

32.76

Weight (kDa)

4.65

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 34 - 64 1.6e-06 zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 269
AciI CCGC 1 cut(s) 177
AclWI GGATC 4 cut(s) 274, 329, 530, 663
AcuI CTGAAG 1 cut(s) 753
AfaI GTAC 2 cut(s) 602, 639
AfiI CCNNNNNNNGG 2 cut(s) 412, 588
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 2 cut(s) 764, 871
AjiI CACGTC 1 cut(s) 351
AjnI CCWGG 3 cut(s) 303, 405, 586
AluBI AGCT 3 cut(s) 324, 749, 774
AluI AGCT 3 cut(s) 324, 749, 774
Alw26I GTCTC 2 cut(s) 65, 491
AlwI GGATC 4 cut(s) 274, 329, 530, 663
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 809
AspA2I CCTAGG 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 415
AvrII CCTAGG 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 269
BarI GAAGNNNNNNTAC 2 cut(s) 584, 616
BbvI GCAGC 1 cut(s) 796
BccI CCATC 5 cut(s) 191, 235, 254, 291, 635
BciT130I CCWGG 3 cut(s) 305, 407, 588
BciVI GTATCC 1 cut(s) 177
BcoDI GTCTC 2 cut(s) 65, 491
BfaI CTAG 3 cut(s) 38, 44, 96
BfmI CTRYAG 3 cut(s) 325, 782, 799
BfuI GTATCC 1 cut(s) 177
BglI GCCNNNNNGGC 2 cut(s) 177, 551
BglII AGATCT 1 cut(s) 40
BisI GCNGC 2 cut(s) 178, 810
BlnI CCTAGG 1 cut(s) 95
BlsI GCNGC 2 cut(s) 179, 811
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 3 cut(s) 305, 407, 588
BmgBI CACGTC 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 3 cut(s) 305, 407, 588
BmsI GCATC 4 cut(s) 454, 521, 657, 707
BsaJI CCNNGG 4 cut(s) 95, 172, 586, 587
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 588
Bse1I ACTGG 2 cut(s) 291, 541
BseBI CCWGG 3 cut(s) 305, 407, 588
BseDI CCNNGG 4 cut(s) 95, 172, 586, 587
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 412, 588
BseMII CTCAG 1 cut(s) 611
BseNI ACTGG 2 cut(s) 291, 541
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 1 cut(s) 796
BshFI GGCC 1 cut(s) 417
BshNI GGYRCC 1 cut(s) 269
BslFI GGGAC 2 cut(s) 337, 705
BslI CCNNNNNNNGG 2 cut(s) 412, 588
BsmAI GTCTC 2 cut(s) 65, 491
BsmFI GGGAC 2 cut(s) 337, 705
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 6 cut(s) 40, 279, 334, 522, 571, 655
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 1 cut(s) 177
BspANI GGCC 1 cut(s) 417
BspCNI CTCAG 1 cut(s) 610
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 786
BspPI GGATC 4 cut(s) 274, 329, 530, 663
BspT107I GGYRCC 1 cut(s) 269
BsrI ACTGG 2 cut(s) 291, 541
BssECI CCNNGG 4 cut(s) 95, 172, 586, 587
BssMI GATC 6 cut(s) 40, 279, 334, 522, 571, 655
BssT1I CCWWGG 2 cut(s) 95, 172
Bst2UI CCWGG 3 cut(s) 305, 407, 588
Bst4CI ACNGT 4 cut(s) 110, 142, 301, 311
BstC8I GCNNGC 2 cut(s) 617, 807
BstDEI CTNAG 3 cut(s) 23, 455, 597
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 6 cut(s) 43, 282, 337, 525, 574, 658
BstMAI GTCTC 2 cut(s) 65, 491
BstMBI GATC 6 cut(s) 40, 279, 334, 522, 571, 655
BstMWI GCNNNNNNNGC 4 cut(s) 168, 177, 551, 560
BstNI CCWGG 3 cut(s) 305, 407, 588
BstNSI RCATGY 4 cut(s) 162, 619, 759, 809
BstSCI CCNGG 3 cut(s) 303, 405, 586
BstSFI CTRYAG 3 cut(s) 325, 782, 799
BstSLI GKGCMC 1 cut(s) 272
BstV1I GCAGC 1 cut(s) 796
BstX2I RGATCY 4 cut(s) 40, 334, 522, 655
BstYI RGATCY 4 cut(s) 40, 334, 522, 655
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 1 cut(s) 417
BtgI CCRYGG 1 cut(s) 172
BtrI CACGTC 1 cut(s) 351
BtsCI GGATG 1 cut(s) 283
BtsI GCAGTG 2 cut(s) 366, 779
BtsIMutI CAGTG 6 cut(s) 122, 138, 316, 366, 534, 779
Cac8I GCNNGC 2 cut(s) 617, 807
CaiI CAGNNNCTG 1 cut(s) 536
CciI TCATGA 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 124
Csp6I GTAC 2 cut(s) 601, 638
CviAII CATG 8 cut(s) 159, 173, 377, 419, 494, 616, 756, 806
CviQI GTAC 2 cut(s) 601, 638
DdeI CTNAG 3 cut(s) 23, 455, 597
DpnI GATC 6 cut(s) 42, 281, 336, 524, 573, 657
DpnII GATC 6 cut(s) 40, 279, 334, 522, 571, 655
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 95, 172
Eco57I CTGAAG 1 cut(s) 753
EcoRII CCWGG 3 cut(s) 303, 405, 586
EcoT14I CCWWGG 2 cut(s) 95, 172
EcoT22I ATGCAT 1 cut(s) 770
ErhI CCWWGG 2 cut(s) 95, 172
FaeI CATG 8 cut(s) 162, 176, 380, 422, 497, 619, 759, 809
FaqI GGGAC 2 cut(s) 337, 705
FatI CATG 8 cut(s) 158, 172, 376, 418, 493, 615, 755, 805
Fnu4HI GCNGC 2 cut(s) 178, 810
FokI GGATG 1 cut(s) 270
Fsp4HI GCNGC 2 cut(s) 178, 810
FspBI CTAG 3 cut(s) 38, 44, 96
GluI GCNGC 2 cut(s) 178, 810
HaeIII GGCC 1 cut(s) 417
HgaI GACGC 1 cut(s) 124
Hin1II CATG 8 cut(s) 162, 176, 380, 422, 497, 619, 759, 809
HinfI GANTC 4 cut(s) 233, 254, 398, 425
Hpy188I TCNGA 5 cut(s) 232, 238, 259, 279, 334
Hpy188III TCNNGA 5 cut(s) 38, 44, 53, 494, 520
HpyAV CCTTC 3 cut(s) 297, 875, 879
HpyCH4III ACNGT 4 cut(s) 110, 142, 301, 311
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 7 cut(s) 12, 467, 512, 648, 698, 768, 784
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 177, 551, 560
HpyF3I CTNAG 3 cut(s) 23, 455, 597
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 8 cut(s) 162, 176, 380, 422, 497, 619, 759, 809
Kzo9I GATC 6 cut(s) 40, 279, 334, 522, 571, 655
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 4 cut(s) 454, 521, 657, 707
MaeI CTAG 3 cut(s) 38, 44, 96
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 4 cut(s) 136, 399, 505, 707
MalI GATC 6 cut(s) 42, 281, 336, 524, 573, 657
MboI GATC 6 cut(s) 40, 279, 334, 522, 571, 655
MboII GAAGA 2 cut(s) 8, 844
MflI RGATCY 4 cut(s) 40, 334, 522, 655
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 2 cut(s) 153, 889
MlyI GAGTC 1 cut(s) 407
MnlI CCTC 8 cut(s) 52, 73, 93, 174, 310, 498, 544, 606
Mph1103I ATGCAT 1 cut(s) 770
MspR9I CCNGG 3 cut(s) 305, 407, 588
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 3 cut(s) 305, 407, 588
MwoI GCNNNNNNNGC 4 cut(s) 168, 177, 551, 560
NcoI CCATGG 1 cut(s) 172
NdeII GATC 6 cut(s) 40, 279, 334, 522, 571, 655
NlaIII CATG 8 cut(s) 162, 176, 380, 422, 497, 619, 759, 809
NlaIV GGNNCC 1 cut(s) 271
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 2 cut(s) 136, 399
NsiI ATGCAT 1 cut(s) 770
NspI RCATGY 4 cut(s) 162, 619, 759, 809
PaeI GCATGC 2 cut(s) 619, 809
PagI TCATGA 1 cut(s) 493
PasI CCCWGGG 1 cut(s) 587
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 3 cut(s) 233, 254, 425
PflFI GACNNNGTC 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 2 cut(s) 179, 811
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Psp6I CCWGG 3 cut(s) 303, 405, 586
PspGI CCWGG 3 cut(s) 303, 405, 586
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 415
PsrI GAACNNNNNNTAC 2 cut(s) 429, 461
PstI CTGCAG 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 4 cut(s) 40, 334, 522, 655
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 2 cut(s) 602, 639
RsaNI GTAC 2 cut(s) 601, 638
SatI GCNGC 2 cut(s) 178, 810
Sau3AI GATC 6 cut(s) 40, 279, 334, 522, 571, 655
Sau96I GGNCC 1 cut(s) 415
ScaI AGTACT 1 cut(s) 602
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 3 cut(s) 305, 407, 588
SduI GDGCHC 1 cut(s) 272
SetI ASST 6 cut(s) 104, 326, 353, 579, 751, 776
SfaNI GCATC 4 cut(s) 454, 521, 657, 707
SfcI CTRYAG 3 cut(s) 325, 782, 799
SphI GCATGC 2 cut(s) 619, 809
Sse9I AATT 2 cut(s) 153, 889
SsiI CCGC 1 cut(s) 177
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 38, 44, 96
StyD4I CCNGG 3 cut(s) 303, 405, 586
StyI CCWWGG 2 cut(s) 95, 172
TaaI ACNGT 4 cut(s) 110, 142, 301, 311
TaiI ACGT 1 cut(s) 353
TaqI TCGA 5 cut(s) 150, 315, 396, 428, 570
TaqII GACCGA 1 cut(s) 505
TasI AATT 2 cut(s) 153, 889
TatI WGTACW 2 cut(s) 600, 637
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 3 cut(s) 233, 254, 425
TscAI CASTG 6 cut(s) 122, 145, 316, 373, 541, 786
TseFI GTSAC 2 cut(s) 136, 399
TseI GCWGC 1 cut(s) 809
Tsp45I GTSAC 2 cut(s) 136, 399
TspDTI ATGAA 2 cut(s) 350, 780
TspRI CASTG 6 cut(s) 122, 145, 316, 373, 541, 786
Tth111I GACNNNGTC 1 cut(s) 311
XbaI TCTAGA 2 cut(s) 37, 43
XceI RCATGY 4 cut(s) 162, 619, 759, 809
XmaJI CCTAGG 1 cut(s) 95
XspI CTAG 3 cut(s) 38, 44, 96
ZrmI AGTACT 1 cut(s) 602
Zsp2I ATGCAT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.