Rorug05G0179200

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002406
Physical Location & Seq
Forward (+)
17060052 .. 17060526
475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0179200.1

Sequence Viewer

Length: 357 bp
ATGTCACCTGAGTATGTCTTTGGAGGGACCTTCTCCGTAAAGTTCGATGTGTACAGTTTTGGAGTCTTAATGCTTGAAATCATAAGTGGTAGGAGAAACAATAAAAACTTCCACGATGATGATTGCACATGGGAGCTATGGAAAAAAGGTGCAGCGCTAGAGCTGATCGATCCAATACTAGGCAATTCCTGTGATGAGGATCAATTGTTAAGATGCATCCATGCCGGTCTGCTCTGTGTGGAAGAGAATGCAACCGATCGGTTGAACATGTCGGATCTCATATCTATGTTGACAAATAAAAGCGTGCCTTTACCTAAACCAACAAAGCCACCATTTTGTACAAAGAGAAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.33

Weight (kDa)

5.3

Isoelectric Point (pI)

58.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 96 2.5e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 164, 207, 282
AfaI GTAC 2 cut(s) 53, 340
AfeI AGCGCT 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 2 cut(s) 77, 265
AluBI AGCT 2 cut(s) 136, 163
AluI AGCT 2 cut(s) 136, 163
AlwI GGATC 3 cut(s) 164, 207, 282
Aor51HI AGCGCT 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 27
AvaII GGWCC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 164
BfaI CTAG 2 cut(s) 158, 179
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 1 cut(s) 28
BmsI GCATC 2 cut(s) 203, 225
Bsa29I ATCGAT 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 198
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse118I RCCGGY 1 cut(s) 224
Bse8I GATNNNNATC 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 168
BseGI GGATG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 259
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 259
BsiSI CCGG 1 cut(s) 225
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 253
Bsp1407I TGTACA 2 cut(s) 51, 338
Bsp143I GATC 5 cut(s) 165, 169, 199, 256, 274
BspDI ATCGAT 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 28
BspPI GGATC 3 cut(s) 164, 207, 282
BsrFI RCCGGY 1 cut(s) 224
BsrGI TGTACA 2 cut(s) 51, 338
BssAI RCCGGY 1 cut(s) 224
BssMI GATC 5 cut(s) 165, 169, 199, 256, 274
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 237
BstAUI TGTACA 2 cut(s) 51, 338
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 216
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 5 cut(s) 168, 172, 202, 259, 277
BstMBI GATC 5 cut(s) 165, 169, 199, 256, 274
BstMCI CGRYCG 1 cut(s) 259
BstNSI RCATGY 1 cut(s) 271
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 168
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 305
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 27
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 2 cut(s) 52, 339
CviAII CATG 3 cut(s) 129, 221, 268
CviJI RGCY 3 cut(s) 136, 163, 328
CviKI_1 RGCY 3 cut(s) 136, 163, 328
CviQI GTAC 2 cut(s) 52, 339
DdeI CTNAG 1 cut(s) 9
DpnI GATC 5 cut(s) 167, 171, 201, 258, 276
DpnII GATC 5 cut(s) 165, 169, 199, 256, 274
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 27
Eco47III AGCGCT 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 218
FaeI CATG 3 cut(s) 132, 224, 271
FaiI YATR 8 cut(s) 15, 83, 130, 139, 222, 269, 281, 287
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FaqI GGGAC 1 cut(s) 40
FatI CATG 3 cut(s) 128, 220, 267
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 2 cut(s) 158, 179
GlaI GCGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 153
HaeII RGCGCY 1 cut(s) 158
HapII CCGG 1 cut(s) 225
HhaI GCGC 1 cut(s) 157
Hin1II CATG 3 cut(s) 132, 224, 271
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 1 cut(s) 63
HpaII CCGG 1 cut(s) 225
Hpy166II GTNNAC 2 cut(s) 52, 291
Hpy188I TCNGA 2 cut(s) 274, 356
Hpy8I GTNNAC 2 cut(s) 52, 291
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 4 cut(s) 126, 152, 216, 251
HpyF3I CTNAG 1 cut(s) 9
Hsp92II CATG 3 cut(s) 132, 224, 271
HspAI GCGC 1 cut(s) 155
Kzo9I GATC 5 cut(s) 165, 169, 199, 256, 274
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 3 cut(s) 21, 202, 238
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 2 cut(s) 203, 225
MaeI CTAG 2 cut(s) 158, 179
MaeIII GTNAC 1 cut(s) 3
MalI GATC 5 cut(s) 167, 171, 201, 258, 276
MboI GATC 5 cut(s) 165, 169, 199, 256, 274
MboII GAAGA 1 cut(s) 254
MfeI CAATTG 1 cut(s) 203
MflI RGATCY 1 cut(s) 274
MluCI AATT 2 cut(s) 184, 203
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 2 cut(s) 17, 190
Mph1103I ATGCAT 1 cut(s) 218
MseI TTAA 2 cut(s) 68, 209
MslI CAYNNNNRTG 2 cut(s) 117, 284
MspI CCGG 1 cut(s) 225
MunI CAATTG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 253
NdeII GATC 5 cut(s) 165, 169, 199, 256, 274
NlaIII CATG 3 cut(s) 132, 224, 271
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 1 cut(s) 218
NspI RCATGY 1 cut(s) 271
PciI ACATGT 1 cut(s) 267
PctI GAATGC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 154
Ple19I CGATCG 1 cut(s) 259
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PpuMI RGGWCCY 1 cut(s) 27
PscI ACATGT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 27
PsuI RGATCY 1 cut(s) 274
PvuI CGATCG 1 cut(s) 259
RsaI GTAC 2 cut(s) 53, 340
RsaNI GTAC 2 cut(s) 52, 339
RseI CAYNNNNRTG 2 cut(s) 117, 284
SaqAI TTAA 2 cut(s) 68, 209
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 5 cut(s) 165, 169, 199, 256, 274
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 1 cut(s) 72
SetI ASST 6 cut(s) 10, 32, 138, 151, 165, 316
SfaNI GCATC 2 cut(s) 203, 225
SinI GGWCC 1 cut(s) 27
SmiMI CAYNNNNRTG 2 cut(s) 117, 284
Sse9I AATT 2 cut(s) 184, 203
SspMI CTAG 2 cut(s) 158, 179
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 2 cut(s) 45, 168
TasI AATT 2 cut(s) 184, 203
TatI WGTACW 2 cut(s) 51, 338
Tru1I TTAA 2 cut(s) 68, 209
Tru9I TTAA 2 cut(s) 68, 209
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 3
TspGWI ACGGA 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 27
XceI RCATGY 1 cut(s) 271
XspI CTAG 2 cut(s) 158, 179
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.