Rw0G005680

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00224
OG0002406
Physical Location & Seq
Reverse (-)
43470 .. 45394
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G005680.1

Sequence Viewer

Length: 945 bp
ATGGCACCACCACCAGCAGCTATTTTCAACCAGGAGAAGTTGCGTGTTGCACTGCTGAGTATCTGTGCACGATACCAATTTATTAACGACAATCTAGATCTAGATAGTCAGGACGAGGAACATACAGAGACAACAGAGCAAGCAAATGTTCCTAGCAGGTATTACTGCTATCAGTGTCGTCAAGTGGTCGGTGTCACTGTGGACGTCGAAATTACATGCCCTCTCTGCCATGGCGGCTGGATACAGGAATATGGTAGCGCTAACTTACTCTATGTTAGTGTGCCATCAGAATCCAAGCATTCTGCTAGTGATTCTGATGGGCAAGGCACCATTCCGGTCAATCCTTCCGGTCAAACCTCCAACAGCTATGGTTCAGATCCCACCCTCAACAGGTCCCCTTTTCATATCACTGCGAACGGTTACAGTAACGTTCAAATTTCCCATGCTTCCCATGCCGAGTCACACCAGGCAACGGGCCATTCTGATTCGAGTTTGGAACAGGGATTTCTATCTGTTGATGCTAACCAGCGGACAAGGGTAACTGCATCAGACTGGATCTGTTCAGCCGTTGGTTTAGCCATTGAGGCTTTATCGGCATTTTTCGATCAGGTTTCCACCCCAGAGACGCCTCAATACTCATTGTATGGCATGCTGTTGTCCATCACATCTGTACCTGTTTGCTGCTTGGACCTCATTTACAATGCTAATGCCCTGATACAAAGAGAGAGAGAGAGAGAGAGAGAACTCGCATCTGCAAGTAACATTCTTTGGGGTATCTTGCCTGAAATTTTTGGATTAGCTCTGGCATGTATTCAATGCATTAGCTCGGCACTGCAGTTTTCATTTCTCTGTAGGCATTCTGCTAATCCTGTGAAAATATCGCTTGTGCCTTTCTTATTTTTCTTCGTTTTGTTTGCTTTCAAATATAGAGAAAGAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
FNIP LRR_8 NB-ARC

Protein Analysis

314

Amino Acids

34.66

Weight (kDa)

5.13

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 52 - 84 3.1e-07 zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 147
AccB1I GGYRCC 2 cut(s) 4, 326
AciI CCGC 2 cut(s) 234, 529
AclI AACGTT 1 cut(s) 429
AclWI GGATC 2 cut(s) 371, 563
AcsI RAATTY 2 cut(s) 435, 786
AcyI GRCGYC 2 cut(s) 204, 626
AfaI GTAC 1 cut(s) 672
AfeI AGCGCT 1 cut(s) 259
AfiI CCNNNNNNNGG 2 cut(s) 390, 472
AgsI TTSAA 4 cut(s) 28, 434, 815, 922
AjnI CCWGG 2 cut(s) 30, 465
AluBI AGCT 4 cut(s) 20, 366, 800, 825
AluI AGCT 4 cut(s) 20, 366, 800, 825
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 2 cut(s) 122, 617
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 2 cut(s) 371, 563
Aor51HI AGCGCT 1 cut(s) 259
AoxI GGCC 1 cut(s) 475
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 17, 681
ApoI RAATTY 2 cut(s) 435, 786
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 3 cut(s) 393, 475, 688
AvaII GGWCC 2 cut(s) 393, 688
BaeGI GKGCMC 1 cut(s) 70
BaeI ACNNNNGTAYC 2 cut(s) 654, 687
BanI GGYRCC 2 cut(s) 4, 326
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 29, 668
BccI CCATC 3 cut(s) 292, 311, 668
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 2 cut(s) 32, 467
BciVI GTATCC 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 122, 617
BfaI CTAG 4 cut(s) 95, 101, 153, 306
BfmI CTRYAG 2 cut(s) 833, 850
BfoI RGCGCY 1 cut(s) 261
BfuAI ACCTGC 1 cut(s) 147
BfuI GTATCC 1 cut(s) 234
BglI GCCNNNNNGGC 2 cut(s) 234, 584
BglII AGATCT 1 cut(s) 97
BisI GCNGC 3 cut(s) 18, 235, 682
BlsI GCNGC 3 cut(s) 19, 236, 683
Bme1390I CCNGG 2 cut(s) 32, 467
Bme18I GGWCC 2 cut(s) 393, 688
BmgT120I GGNCC 3 cut(s) 393, 475, 688
BmiI GGNNCC 3 cut(s) 6, 328, 395
BmrFI CCNGG 2 cut(s) 32, 467
BmsI GCATC 3 cut(s) 508, 554, 758
BsaBI GATNNNNATC 1 cut(s) 508
BsaHI GRCGYC 2 cut(s) 204, 626
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 2 cut(s) 334, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 472
Bse1I ACTGG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 508
BseBI CCWGG 2 cut(s) 32, 467
BseDI CCNNGG 1 cut(s) 229
BseJI GATNNNNATC 1 cut(s) 508
BseLI CCNNNNNNNGG 2 cut(s) 390, 472
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 557
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 2 cut(s) 29, 668
BshFI GGCC 1 cut(s) 477
BshNI GGYRCC 2 cut(s) 4, 326
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 2 cut(s) 335, 348
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 390, 472
BsmAI GTCTC 2 cut(s) 122, 617
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 2 cut(s) 298, 856
BsnI GGCC 1 cut(s) 477
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 4 cut(s) 97, 376, 555, 604
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 2 cut(s) 234, 529
BspANI GGCC 1 cut(s) 477
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 6, 328, 395
BspMAI CTGCAG 1 cut(s) 837
BspMI ACCTGC 1 cut(s) 147
BspPI GGATC 2 cut(s) 371, 563
BspT107I GGYRCC 2 cut(s) 4, 326
BsrI ACTGG 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 4 cut(s) 97, 376, 555, 604
BssNI GRCGYC 2 cut(s) 204, 626
BssT1I CCWWGG 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 32, 467
Bst4CI ACNGT 3 cut(s) 199, 419, 425
BstACI GRCGYC 2 cut(s) 204, 626
BstC8I GCNNGC 2 cut(s) 141, 650
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 4 cut(s) 100, 379, 558, 607
BstMAI GTCTC 2 cut(s) 122, 617
BstMBI GATC 4 cut(s) 97, 376, 555, 604
BstMWI GCNNNNNNNGC 5 cut(s) 225, 234, 452, 584, 593
BstNI CCWGG 2 cut(s) 32, 467
BstNSI RCATGY 3 cut(s) 219, 652, 810
BstSCI CCNGG 2 cut(s) 30, 465
BstSFI CTRYAG 2 cut(s) 833, 850
BstSLI GKGCMC 1 cut(s) 70
BstV1I GCAGC 2 cut(s) 29, 668
BstX2I RGATCY 3 cut(s) 97, 376, 555
BstYI RGATCY 3 cut(s) 97, 376, 555
BsuI GTATCC 1 cut(s) 234
BsuRI GGCC 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 229
BtsI GCAGTG 3 cut(s) 50, 408, 830
BtsIMutI CAGTG 5 cut(s) 50, 179, 195, 408, 830
BveI ACCTGC 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 141, 650
CfoI GCGC 1 cut(s) 260
Cfr13I GGNCC 3 cut(s) 393, 475, 688
CseI GACGC 1 cut(s) 634
Csp6I GTAC 1 cut(s) 671
CviAII CATG 6 cut(s) 216, 230, 443, 452, 649, 807
CviJI RGCY 9 cut(s) 20, 237, 366, 477, 566, 578, 587, 800, 825
CviKI_1 RGCY 9 cut(s) 20, 237, 366, 477, 566, 578, 587, 800, 825
CviQI GTAC 1 cut(s) 671
DdeI CTNAG 1 cut(s) 56
DpnI GATC 4 cut(s) 99, 378, 557, 606
DpnII GATC 4 cut(s) 97, 376, 555, 604
Eco130I CCWWGG 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 393, 688
Eco47III AGCGCT 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 393
EcoRII CCWGG 2 cut(s) 30, 465
EcoT14I CCWWGG 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 821
ErhI CCWWGG 1 cut(s) 229
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 6 cut(s) 219, 233, 446, 455, 652, 810
FaqI GGGAC 1 cut(s) 379
FatI CATG 6 cut(s) 215, 229, 442, 451, 648, 806
Fnu4HI GCNGC 3 cut(s) 18, 235, 682
Fsp4HI GCNGC 3 cut(s) 18, 235, 682
FspBI CTAG 4 cut(s) 95, 101, 153, 306
GlaI GCGC 1 cut(s) 259
GluI GCNGC 3 cut(s) 18, 235, 682
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 1 cut(s) 477
HapII CCGG 2 cut(s) 335, 348
HgaI GACGC 1 cut(s) 634
HhaI GCGC 1 cut(s) 260
Hin1I GRCGYC 2 cut(s) 204, 626
Hin1II CATG 6 cut(s) 219, 233, 446, 455, 652, 810
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HinfI GANTC 4 cut(s) 290, 311, 458, 485
HpaII CCGG 2 cut(s) 335, 348
Hpy166II GTNNAC 2 cut(s) 68, 202
Hpy188I TCNGA 5 cut(s) 289, 316, 376, 484, 550
Hpy188III TCNNGA 3 cut(s) 95, 101, 110
Hpy8I GTNNAC 2 cut(s) 68, 202
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 354, 930
HpyCH4III ACNGT 3 cut(s) 199, 419, 425
HpyCH4IV ACGT 2 cut(s) 204, 429
HpyCH4V TGCA 6 cut(s) 50, 68, 545, 755, 819, 835
HpyF10VI GCNNNNNNNGC 5 cut(s) 225, 234, 452, 584, 593
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 2 cut(s) 204, 429
Hsp92I GRCGYC 2 cut(s) 204, 626
Hsp92II CATG 6 cut(s) 219, 233, 446, 455, 652, 810
HspAI GCGC 1 cut(s) 258
Kzo9I GATC 4 cut(s) 97, 376, 555, 604
Lsp1109I GCAGC 2 cut(s) 29, 668
LweI GCATC 3 cut(s) 508, 554, 758
MaeI CTAG 4 cut(s) 95, 101, 153, 306
MaeII ACGT 2 cut(s) 204, 429
MaeIII GTNAC 6 cut(s) 193, 419, 425, 459, 538, 758
MalI GATC 4 cut(s) 99, 378, 557, 606
MboI GATC 4 cut(s) 97, 376, 555, 604
MboII GAAGA 1 cut(s) 895
MflI RGATCY 3 cut(s) 97, 376, 555
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 5 cut(s) 77, 210, 435, 786, 940
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 7 cut(s) 109, 231, 367, 395, 577, 639, 701
Mph1103I ATGCAT 1 cut(s) 821
MseI TTAA 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 529
MspI CCGG 2 cut(s) 335, 348
MspR9I CCNGG 2 cut(s) 32, 467
Mva1269I GAATGC 2 cut(s) 298, 856
MvaI CCWGG 2 cut(s) 32, 467
MwoI GCNNNNNNNGC 5 cut(s) 225, 234, 452, 584, 593
NcoI CCATGG 1 cut(s) 229
NdeII GATC 4 cut(s) 97, 376, 555, 604
NlaIII CATG 6 cut(s) 219, 233, 446, 455, 652, 810
NlaIV GGNNCC 3 cut(s) 6, 328, 395
NmeAIII GCCGAG 2 cut(s) 481, 806
NmuCI GTSAC 2 cut(s) 193, 459
NsiI ATGCAT 1 cut(s) 821
NspI RCATGY 3 cut(s) 219, 652, 810
PaeI GCATGC 1 cut(s) 652
PctI GAATGC 2 cut(s) 298, 856
PfeI GAWTC 3 cut(s) 290, 311, 485
PkrI GCNGC 3 cut(s) 19, 236, 683
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PpuMI RGGWCCY 1 cut(s) 393
Psp1406I AACGTT 1 cut(s) 429
Psp5II RGGWCCY 1 cut(s) 393
Psp6I CCWGG 2 cut(s) 30, 465
PspGI CCWGG 2 cut(s) 30, 465
PspN4I GGNNCC 3 cut(s) 6, 328, 395
PspPI GGNCC 3 cut(s) 393, 475, 688
PspPPI RGGWCCY 1 cut(s) 393
PstI CTGCAG 1 cut(s) 837
PsuI RGATCY 3 cut(s) 97, 376, 555
RsaI GTAC 1 cut(s) 672
RsaNI GTAC 1 cut(s) 671
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 3 cut(s) 18, 235, 682
Sau3AI GATC 4 cut(s) 97, 376, 555, 604
Sau96I GGNCC 3 cut(s) 393, 475, 688
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 2 cut(s) 32, 467
SduI GDGCHC 1 cut(s) 70
SfaNI GCATC 3 cut(s) 508, 554, 758
SfcI CTRYAG 2 cut(s) 833, 850
SinI GGWCC 2 cut(s) 393, 688
SphI GCATGC 1 cut(s) 652
Sse9I AATT 5 cut(s) 77, 210, 435, 786, 940
SsiI CCGC 2 cut(s) 234, 529
SspMI CTAG 4 cut(s) 95, 101, 153, 306
StyD4I CCNGG 2 cut(s) 30, 465
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 3 cut(s) 199, 419, 425
TaiI ACGT 2 cut(s) 207, 432
TaqI TCGA 3 cut(s) 207, 488, 603
TasI AATT 5 cut(s) 77, 210, 435, 786, 940
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 290, 311, 485
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 5 cut(s) 57, 179, 202, 415, 837
TseFI GTSAC 2 cut(s) 193, 459
TseI GCWGC 2 cut(s) 17, 681
Tsp45I GTSAC 2 cut(s) 193, 459
TspDTI ATGAA 2 cut(s) 392, 831
TspRI CASTG 5 cut(s) 57, 179, 202, 415, 837
VneI GTGCAC 1 cut(s) 66
VpaK11BI GGWCC 2 cut(s) 393, 688
XapI RAATTY 2 cut(s) 435, 786
XbaI TCTAGA 2 cut(s) 94, 100
XceI RCATGY 3 cut(s) 219, 652, 810
XspI CTAG 4 cut(s) 95, 101, 153, 306
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.