Rroxscaffold_1G00041690

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002406
Physical Location & Seq
Forward (+)
59607465 .. 59610162
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041690.1

Sequence Viewer

Length: 1116 bp
ATGGATAGCGGCTTGAGTGAGAGCTTGATCGGAGTAGCACCACCACCACCACTATCGTCGGAGACGGCTAGCCAGAGATGGAGGAGATTGTGTGGCCTCGTCAAGAACCGCAAGCCAAGCAACTTTAGCTTATTCAATCCTAATCTCTCTAACCGTTACGACCAACAAGCTCCAGCAGCTATTATCAACCAGGAGAAGTTCCGTGTTGCAGTGCTGACTATATATGCACAATACCAATTTATTAACGGAATAGACAATCTAGATCTAGATAGTCAGGACGAGGAAAAAACAGAGACAACAGAGGAAGCAAATGTTCCTAGGAGGTATTACTGTTATCAGTGTCGTCAAGTGGTCAGCGTCACTGTGGAAGTCGAAATTACATGCCCTCTCTGCCATGGCGGCTGGATACAAGAATGTGATGGCGTTAGCATACTCTATCTTGCTATGCCATCAGAATCCGAGCATTCGGCAAGTGATTCTGATGGACAGGGCACCATTCTGATCCATCCTTCCAGTCAAACTGTCCTGGACAGTGTCGAGGACAGCTATAGTTCAGATCCCAACCCCAACACGTCCCCTTTTCATAGCACTGCTTCCCATGATTACGACAACTATGTCGAGTCACACCAGGCAACGGGCCATGATGATTCGAGTTTGGAACAGGGATTACTAACACTTAGCATTGATGCAAATATTGTTGCTAACCAGCCTCATCATGAGACAAGGGTTACTGCATCGGTCTGGATCTTTTCAGCCACTGGTTTAGCCTTTGAGGCTGTATCGGCATTTTTCGATCAGGTTTCCACCCCAGGGAAGCCTCAGTACTCGTTGTATGGCATGCTATTGTCCATCGTGGCTGTACTTGTTTGCATCTTGGATCTCATTCACAATGCCCTGATACAAAGACAAAGGGGACTTGCATCTGCGAGTAACATTATTTGGGGTATCTTGCCTGAAGTTTTTGGATTAGCTCTCGCATGTATTCAATGCATTAGCTCGGCACTGCAGTTTTCATTTCTCTGTAGGCATGCTGCTAATCCTGTGAAAATATCGCTTGTGCCTTTCTTATTTTTCTTCGTTTTGTTTGCTTTCAAATATAGAGAAGGAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

371

Amino Acids

40.99

Weight (kDa)

4.95

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 108 - 138 2.2e-06 zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 491
AciI CCGC 3 cut(s) 9, 109, 399
AclWI GGATC 4 cut(s) 496, 551, 752, 885
AcuI CTGAAG 1 cut(s) 975
AfaI GTAC 2 cut(s) 824, 861
AfiI CCNNNNNNNGG 2 cut(s) 634, 810
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 3 cut(s) 136, 986, 1093
AjiI CACGTC 1 cut(s) 573
AjnI CCWGG 4 cut(s) 189, 525, 627, 808
AluBI AGCT 7 cut(s) 24, 129, 170, 179, 546, 971, 996
AluI AGCT 7 cut(s) 24, 129, 170, 179, 546, 971, 996
Alw26I GTCTC 3 cut(s) 56, 287, 713
AlwI GGATC 4 cut(s) 496, 551, 752, 885
AlwNI CAGNNNCTG 1 cut(s) 758
AoxI GGCC 2 cut(s) 94, 637
ApeKI GCWGC 2 cut(s) 176, 1031
AspA2I CCTAGG 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 637
AsuNHI GCTAGC 1 cut(s) 68
AvrII CCTAGG 1 cut(s) 317
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 1 cut(s) 491
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BbvI GCAGC 2 cut(s) 188, 1018
BccI CCATC 6 cut(s) 72, 413, 457, 476, 513, 857
BceAI ACGGC 1 cut(s) 81
BciT130I CCWGG 4 cut(s) 191, 527, 629, 810
BciVI GTATCC 1 cut(s) 399
BcoDI GTCTC 3 cut(s) 56, 287, 713
BfaI CTAG 4 cut(s) 69, 260, 266, 318
BfmI CTRYAG 3 cut(s) 547, 1004, 1021
BfuI GTATCC 1 cut(s) 399
BglI GCCNNNNNGGC 2 cut(s) 399, 773
BglII AGATCT 1 cut(s) 262
BisI GCNGC 4 cut(s) 10, 177, 400, 1032
BlnI CCTAGG 1 cut(s) 317
BlsI GCNGC 4 cut(s) 11, 178, 401, 1033
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 4 cut(s) 191, 527, 629, 810
BmgBI CACGTC 1 cut(s) 573
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 1 cut(s) 493
BmrFI CCNGG 4 cut(s) 191, 527, 629, 810
BmsI GCATC 4 cut(s) 676, 743, 879, 929
BmtI GCTAGC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 34
BsaJI CCNNGG 4 cut(s) 317, 394, 808, 809
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 2 cut(s) 634, 810
Bse1I ACTGG 2 cut(s) 513, 763
BseBI CCWGG 4 cut(s) 191, 527, 629, 810
BseDI CCNNGG 4 cut(s) 317, 394, 808, 809
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 2 cut(s) 634, 810
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 2 cut(s) 513, 763
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 2 cut(s) 188, 1018
BshFI GGCC 2 cut(s) 96, 639
BshNI GGYRCC 1 cut(s) 491
BslFI GGGAC 2 cut(s) 559, 927
BslI CCNNNNNNNGG 2 cut(s) 634, 810
BsmAI GTCTC 3 cut(s) 56, 287, 713
BsmBI CGTCTC 1 cut(s) 56
BsmFI GGGAC 2 cut(s) 559, 927
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 2 cut(s) 96, 639
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 3 cut(s) 9, 109, 399
BspANI GGCC 2 cut(s) 96, 639
BspCNI CTCAG 1 cut(s) 832
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 493
BspMAI CTGCAG 1 cut(s) 1008
BspOI GCTAGC 1 cut(s) 72
BspPI GGATC 4 cut(s) 496, 551, 752, 885
BspT107I GGYRCC 1 cut(s) 491
BsrI ACTGG 2 cut(s) 513, 763
BssECI CCNNGG 4 cut(s) 317, 394, 808, 809
BssMI GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
BssT1I CCWWGG 2 cut(s) 317, 394
Bst2UI CCWGG 4 cut(s) 191, 527, 629, 810
Bst4CI ACNGT 5 cut(s) 155, 332, 364, 523, 533
BstC8I GCNNGC 4 cut(s) 70, 113, 839, 1029
BstDEI CTNAG 2 cut(s) 677, 819
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 505
BstKTI GATC 7 cut(s) 30, 265, 504, 559, 747, 796, 880
BstMAI GTCTC 3 cut(s) 56, 287, 713
BstMBI GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
BstMWI GCNNNNNNNGC 7 cut(s) 117, 126, 176, 390, 399, 773, 782
BstNI CCWGG 4 cut(s) 191, 527, 629, 810
BstNSI RCATGY 4 cut(s) 384, 841, 981, 1031
BstSCI CCNGG 4 cut(s) 189, 525, 627, 808
BstSFI CTRYAG 3 cut(s) 547, 1004, 1021
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 2 cut(s) 188, 1018
BstX2I RGATCY 4 cut(s) 262, 556, 744, 877
BstYI RGATCY 4 cut(s) 262, 556, 744, 877
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 2 cut(s) 96, 639
BtgI CCRYGG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 573
BtsCI GGATG 1 cut(s) 505
BtsI GCAGTG 3 cut(s) 216, 588, 1001
BtsIMutI CAGTG 7 cut(s) 216, 344, 360, 538, 588, 756, 1001
Cac8I GCNNGC 4 cut(s) 70, 113, 839, 1029
CaiI CAGNNNCTG 1 cut(s) 758
CciI TCATGA 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 637
CseI GACGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 823, 860
CviAII CATG 8 cut(s) 381, 395, 599, 641, 716, 838, 978, 1028
CviQI GTAC 2 cut(s) 823, 860
DdeI CTNAG 2 cut(s) 677, 819
DpnI GATC 7 cut(s) 29, 264, 503, 558, 746, 795, 879
DpnII GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
Eco130I CCWWGG 2 cut(s) 317, 394
Eco57I CTGAAG 1 cut(s) 975
EcoRII CCWGG 4 cut(s) 189, 525, 627, 808
EcoT14I CCWWGG 2 cut(s) 317, 394
EcoT22I ATGCAT 1 cut(s) 992
ErhI CCWWGG 2 cut(s) 317, 394
Esp3I CGTCTC 1 cut(s) 56
FaeI CATG 8 cut(s) 384, 398, 602, 644, 719, 841, 981, 1031
FaqI GGGAC 2 cut(s) 559, 927
FatI CATG 8 cut(s) 380, 394, 598, 640, 715, 837, 977, 1027
Fnu4HI GCNGC 4 cut(s) 10, 177, 400, 1032
FokI GGATG 1 cut(s) 492
Fsp4HI GCNGC 4 cut(s) 10, 177, 400, 1032
FspBI CTAG 4 cut(s) 69, 260, 266, 318
GluI GCNGC 4 cut(s) 10, 177, 400, 1032
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 2 cut(s) 96, 639
HgaI GACGC 1 cut(s) 346
Hin1II CATG 8 cut(s) 384, 398, 602, 644, 719, 841, 981, 1031
HinfI GANTC 4 cut(s) 455, 476, 620, 647
Hpy188I TCNGA 7 cut(s) 32, 61, 454, 460, 481, 501, 556
Hpy188III TCNNGA 6 cut(s) 103, 260, 266, 275, 716, 742
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 3 cut(s) 519, 1097, 1101
HpyCH4III ACNGT 5 cut(s) 155, 332, 364, 523, 533
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 8 cut(s) 209, 227, 689, 734, 870, 920, 990, 1006
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 126, 176, 390, 399, 773, 782
HpyF3I CTNAG 2 cut(s) 677, 819
HpySE526I ACGT 1 cut(s) 572
Hsp92II CATG 8 cut(s) 384, 398, 602, 644, 719, 841, 981, 1031
Kzo9I GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
LmnI GCTCC 1 cut(s) 175
Lsp1109I GCAGC 2 cut(s) 188, 1018
LweI GCATC 4 cut(s) 676, 743, 879, 929
MaeI CTAG 4 cut(s) 69, 260, 266, 318
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 5 cut(s) 155, 358, 621, 727, 929
MalI GATC 7 cut(s) 29, 264, 503, 558, 746, 795, 879
MboI GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
MboII GAAGA 1 cut(s) 1066
MflI RGATCY 4 cut(s) 262, 556, 744, 877
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 3 cut(s) 236, 375, 1111
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 1 cut(s) 39
Mph1103I ATGCAT 1 cut(s) 992
MseI TTAA 1 cut(s) 243
MspR9I CCNGG 4 cut(s) 191, 527, 629, 810
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 4 cut(s) 191, 527, 629, 810
MwoI GCNNNNNNNGC 7 cut(s) 117, 126, 176, 390, 399, 773, 782
NcoI CCATGG 1 cut(s) 394
NdeII GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
NheI GCTAGC 1 cut(s) 68
NlaIII CATG 8 cut(s) 384, 398, 602, 644, 719, 841, 981, 1031
NlaIV GGNNCC 1 cut(s) 493
NmeAIII GCCGAG 1 cut(s) 977
NmuCI GTSAC 2 cut(s) 358, 621
NsiI ATGCAT 1 cut(s) 992
NspI RCATGY 4 cut(s) 384, 841, 981, 1031
PaeI GCATGC 2 cut(s) 841, 1031
PagI TCATGA 1 cut(s) 715
PasI CCCWGGG 1 cut(s) 809
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 3 cut(s) 455, 476, 647
PflFI GACNNNGTC 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 525
PkrI GCNGC 4 cut(s) 11, 178, 401, 1033
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
Psp6I CCWGG 4 cut(s) 189, 525, 627, 808
PspGI CCWGG 4 cut(s) 189, 525, 627, 808
PspN4I GGNNCC 1 cut(s) 493
PspPI GGNCC 1 cut(s) 637
PsrI GAACNNNNNNTAC 2 cut(s) 651, 683
PstI CTGCAG 1 cut(s) 1008
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 4 cut(s) 262, 556, 744, 877
PsyI GACNNNGTC 1 cut(s) 533
RsaI GTAC 2 cut(s) 824, 861
RsaNI GTAC 2 cut(s) 823, 860
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 10, 177, 400, 1032
Sau3AI GATC 7 cut(s) 27, 262, 501, 556, 744, 793, 877
Sau96I GGNCC 1 cut(s) 637
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 4 cut(s) 191, 527, 629, 810
SduI GDGCHC 1 cut(s) 494
SfaNI GCATC 4 cut(s) 676, 743, 879, 929
SfcI CTRYAG 3 cut(s) 547, 1004, 1021
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
SphI GCATGC 2 cut(s) 841, 1031
Sse9I AATT 3 cut(s) 236, 375, 1111
SsiI CCGC 3 cut(s) 9, 109, 399
SspI AATATT 1 cut(s) 694
SspMI CTAG 4 cut(s) 69, 260, 266, 318
StyD4I CCNGG 4 cut(s) 189, 525, 627, 808
StyI CCWWGG 2 cut(s) 317, 394
TaaI ACNGT 5 cut(s) 155, 332, 364, 523, 533
TaiI ACGT 1 cut(s) 575
TaqI TCGA 5 cut(s) 372, 537, 618, 650, 792
TaqII GACCGA 1 cut(s) 727
TasI AATT 3 cut(s) 236, 375, 1111
TatI WGTACW 2 cut(s) 822, 859
TauI GCSGC 2 cut(s) 12, 402
TfiI GAWTC 3 cut(s) 455, 476, 647
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 7 cut(s) 216, 344, 367, 538, 595, 763, 1008
TseFI GTSAC 2 cut(s) 358, 621
TseI GCWGC 2 cut(s) 176, 1031
Tsp45I GTSAC 2 cut(s) 358, 621
TspDTI ATGAA 2 cut(s) 572, 1002
TspGWI ACGGA 2 cut(s) 191, 261
TspRI CASTG 7 cut(s) 216, 344, 367, 538, 595, 763, 1008
Tth111I GACNNNGTC 1 cut(s) 533
XbaI TCTAGA 2 cut(s) 259, 265
XceI RCATGY 4 cut(s) 384, 841, 981, 1031
XmaJI CCTAGG 1 cut(s) 317
XspI CTAG 4 cut(s) 69, 260, 266, 318
ZrmI AGTACT 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.