Rroxscaffold_3G00233550

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000490
Physical Location & Seq
Reverse (-)
19592208 .. 19595434
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233550.1

Sequence Viewer

Length: 1374 bp
ATGGCAAGGTTTTATGGTCCAATTTACAAACTCCAACTTGGTACCAAGTTGGGCATTGTGGTGAGCTCACCTGAGCTTATGAAACAAGTGGTTCGTGACCATGACATAACATTTTCCAACCGTGACTCTACAATTGTTGGTCTAGTCGGGTCATATGGAGCATCAGACATTGCATTTGGATCGTATGGTTCAGATTGGAGGAAGCTGCGCAAGGTGTTTGTGGGTCAGATGCTAAGCAAAATCAACCTTGATGATGGCTATGCCCTGAGAAAAGAGGAGGTGCACAAGTCGATCAGTCATATTTATCATGACAAGATTGGAACCCAAACTGATTTGAGCGAGTTTTCATTTTCCACAGTATATAACGCAACTTTGCGTATGCTATGGGGTGAGAGTCAGCTATGGGGTGAGAGACAAGGGACTGATTTTGGTGAAGATTATAGGAAACTGGTGGCAGAAATTGTATATCTATTTGGGAAACCAAACATTTCGGACTATTTTCCTGTGCTTGCAAGGTTTGACATACAAGGAATTGAGAGGCAAGCAAAGAAAGTTCAATCCGCGATTGACAAGATTCTAAGTTGTGCCATAGAAGAACGGATGAAGATGCTGGCCTCAGACAAAAATGGGGGAATACAACAAAAACATGAAAGGAAGGACTTTCTGCAGTTCCTCTTGGAGAACAATAATAATCATGAAGATGGTTCAACATCCTTGACAGTGCAACAACTGAAGGGCTTGCTCATAGATATCGTGGTGGGTGGAACTGACACCACGGCAACACTAGTGGAATGGGTTCTGACTGAGCTGATGCAGCACCCAGAAGAAATGATAAGAGTTCAAGAAGAACTAACACAAATTGTGGGGTTGAACAACTTGGTTGAAGAGTTTCATTTCCCGAAATTACATCACTTAGATGCTGTGATCAAGGAGTCATTTCGCTTGCACCCTCCAGCGCCCCTTCTAGTGCCCCGGCGTTCAAGCCAACCTGCCATCATTGGTGGCTATTACATACCAAAAGGTTCCACTGTTTTTCTCAATGCTTGGGCCATACACAGAGATCCAAATGTTTGGGATGATCCCTTGGAATTTAGACCCCAGAGGTTCCTAAATCCAAGCAATAGCTTTGATTACCAGGGCAACAAATTTCAGTTTCTTCCATTTGGTTCTGGGAGGAGAATATGTGCCGGGATTCCCTTAGCAGAGAGGATGTTGTTCTATGCGTTGGCTTCATTCTTGCATTCGTTTGATTGGAAATTGCCTAATGACGAAAAGCTTGACGTTTCAGACAAATTTGGGATTGTGACAAAGAAAAACACTCCACTTATTGCTGTACCAACACCCAGGTTATCTAATTTGGAGCTCTATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

457

Amino Acids

52.0

Weight (kDa)

6.51

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 440 2.6e-95 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000490)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10240 FvH4_5g00571 FvH4_5g00571 FvH4_5g00571 FvH4_5g00572 FvH4_5g00572 FvH4_5g00572 FvH4_5g00573 FvH4_5g37440 FvH4_5g37450
prunus_persica Prupe.5G078400_v2.0.a1 Prupe.5G078500_v2.0.a1 Prupe.5G078600_v2.0.a1 Prupe.5G078800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0419931 RchiOBHm_Chr4g0419951 RchiOBHm_Chr4g0420051 RchiOBHm_Chr7g0201681 RchiOBHm_Chr7g0201771 RchiOBHm_Chr7g0201781 RchiOBHm_Chr7g0201791 RchiOBHm_Chr7g0225701 RchiOBHm_Chr7g0225731 RchiOBHm_Chr7g0225761 RchiOBHm_Chr7g0225791 RchiOBHm_Chr7g0225861
rosa_laevigata RLG00000001786 RLG00000001795 RLG00000003649 RLG00000003650 RLG00000003651 RLG00000003652 RLG00000003655 RLG00000003656 RLG00000003657
rosa_multiflora Rmu_sc0001030.1_g000001 Rmu_sc0001030.1_g000005 Rmu_sc0001030.1_g000006 Rmu_sc0006133.1_g000002 Rmu_sc0006637.1_g000005 Rmu_sc0012416.1_g000003 Rmu_sc0016902.1_g000001 Rmu_sc0025481.1_g000003
rosa_roxburghii Rroxscaffold_3G00233490 Rroxscaffold_3G00233550 Rroxscaffold_3G00233560 Rroxscaffold_3G00255100 Rroxscaffold_3G00255110
rosa_rugosa Rorug07G0065300 Rorug07G0065400 Rorug07G0065500 Rorug07G0065600 Rorug07G0065600 Rorug07G0065600 Rorug07G0065700 Rorug07G0065800 Rorug07G0065900 Rorug07G0232900
rosa_samantha Rh3AG071400 Rh3BG286400 Rh3CG072700 Rh4BG225100 Rh4CG235600 Rh5CG585000 Rh7AG192600 Rh7AG193000 Rh7AG193100 Rh7AG193300 Rh7AG371100 Rh7AG387700 Rh7BG194700 Rh7BG194900 Rh7BG195000 Rh7BG366600 Rh7CG203800 Rh7CG204000 Rh7CG204300 Rh7CG389700 Rh7CG407300 Rh7DG198100 Rh7DG198900 Rh7DG199100 Rh7DG377600
rosa_wichuraiana Rw0G020990 Rw7G016880 Rw7G016890 Rw7G028030 Rw7G031840 Rw7G031890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 997
Acc65I GGTACC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 41
AccII CGCG 1 cut(s) 563
AciI CCGC 1 cut(s) 561
AclWI GGATC 3 cut(s) 187, 1055, 1073
AcsI RAATTY 3 cut(s) 1088, 1145, 1292
AcuI CTGAAG 1 cut(s) 752
AfaI GTAC 2 cut(s) 43, 1335
AgsI TTSAA 6 cut(s) 557, 708, 842, 871, 884, 981
AhlI ACTAGT 1 cut(s) 784
AjnI CCWGG 2 cut(s) 1134, 1343
AluBI AGCT 8 cut(s) 66, 76, 205, 400, 808, 1125, 1276, 1363
AluI AGCT 8 cut(s) 66, 76, 205, 400, 808, 1125, 1276, 1363
Alw21I GWGCWC 3 cut(s) 68, 285, 1365
Alw26I GTCTC 1 cut(s) 406
Alw44I GTGCAC 1 cut(s) 281
AlwI GGATC 3 cut(s) 187, 1055, 1073
AoxI GGCC 2 cut(s) 612, 1047
ApaLI GTGCAC 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 205, 814
ApoI RAATTY 3 cut(s) 1088, 1145, 1292
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
Asp700I GAANNNNTTC 2 cut(s) 795, 888
Asp718I GGTACC 1 cut(s) 41
AspLEI GCGC 2 cut(s) 210, 958
AspS9I GGNCC 2 cut(s) 17, 1047
AsuC2I CCSGG 2 cut(s) 973, 1189
AsuHPI GGTGA 5 cut(s) 60, 73, 401, 419, 443
AvaII GGWCC 1 cut(s) 17
BaeGI GKGCMC 2 cut(s) 285, 972
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 2 cut(s) 68, 1365
Bbv12I GWGCWC 3 cut(s) 68, 285, 1365
BbvI GCAGC 2 cut(s) 192, 826
BccI CCATC 3 cut(s) 248, 695, 1001
BceAI ACGGC 1 cut(s) 792
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 2 cut(s) 1136, 1345
BclI TGATCA 1 cut(s) 924
BcnI CCSGG 2 cut(s) 973, 1189
BcoDI GTCTC 1 cut(s) 406
BcuI ACTAGT 1 cut(s) 784
BfaI CTAG 3 cut(s) 143, 785, 965
BfmI CTRYAG 1 cut(s) 665
BfoI RGCGCY 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 997
BisI GCNGC 2 cut(s) 206, 815
BlpI GCTNAGC 1 cut(s) 233
BlsI GCNGC 2 cut(s) 207, 816
Bme1390I CCNGG 4 cut(s) 973, 1136, 1189, 1345
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 17, 1047
BmiI GGNNCC 4 cut(s) 43, 322, 1024, 1106
BmrFI CCNGG 4 cut(s) 973, 1136, 1189, 1345
BmsI GCATC 5 cut(s) 170, 219, 597, 801, 907
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 2 cut(s) 72, 1198
Bpu1102I GCTNAGC 1 cut(s) 233
BpuMI CCSGG 2 cut(s) 973, 1189
BsaJI CCNNGG 5 cut(s) 774, 971, 1083, 1135, 1343
BsaXI ACNNNNNCTCC 2 cut(s) 1168, 1198
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 2 cut(s) 1136, 1345
BseDI CCNNGG 5 cut(s) 774, 971, 1083, 1135, 1343
BseGI GGATG 4 cut(s) 606, 710, 1081, 1215
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 4 cut(s) 63, 257, 630, 795
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 2 cut(s) 290, 1189
BseSI GKGCMC 2 cut(s) 285, 972
BseXI GCAGC 2 cut(s) 192, 826
Bsh1236I CGCG 1 cut(s) 563
BshFI GGCC 2 cut(s) 614, 1049
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 3 cut(s) 68, 285, 1365
BsiSI CCGG 2 cut(s) 973, 1188
BslFI GGGAC 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 433
BsmI GAATGC 1 cut(s) 1240
BsnI GGCC 2 cut(s) 614, 1049
Bsp1286I GDGCHC 4 cut(s) 68, 285, 972, 1365
Bsp143I GATC 5 cut(s) 179, 291, 924, 1060, 1078
Bsp1720I GCTNAGC 1 cut(s) 233
BspACI CCGC 1 cut(s) 561
BspANI GGCC 2 cut(s) 614, 1049
BspCNI CTCAG 4 cut(s) 64, 258, 629, 796
BspFNI CGCG 1 cut(s) 563
BspHI TCATGA 2 cut(s) 307, 694
BspLI GGNNCC 4 cut(s) 43, 322, 1024, 1106
BspMAI CTGCAG 1 cut(s) 669
BspMI ACCTGC 1 cut(s) 997
BspPI GGATC 3 cut(s) 187, 1055, 1073
BspT107I GGYRCC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 1 cut(s) 453
BssECI CCNNGG 5 cut(s) 774, 971, 1083, 1135, 1343
BssMI GATC 5 cut(s) 179, 291, 924, 1060, 1078
BssT1I CCWWGG 1 cut(s) 1083
Bst2UI CCWGG 2 cut(s) 1136, 1345
Bst4CI ACNGT 4 cut(s) 122, 358, 721, 1030
Bst6I CTCTTC 1 cut(s) 879
BstC8I GCNNGC 5 cut(s) 510, 543, 612, 740, 944
BstDEI CTNAG 8 cut(s) 72, 233, 266, 578, 616, 804, 913, 1198
BstDSI CCRYGG 1 cut(s) 774
BstF5I GGATG 4 cut(s) 606, 710, 1081, 1215
BstFNI CGCG 1 cut(s) 563
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 2 cut(s) 210, 958
BstKTI GATC 5 cut(s) 182, 294, 927, 1063, 1081
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 5 cut(s) 179, 291, 924, 1060, 1078
BstMWI GCNNNNNNNGC 1 cut(s) 814
BstNI CCWGG 2 cut(s) 1136, 1345
BstSCI CCNGG 4 cut(s) 971, 1134, 1187, 1343
BstSFI CTRYAG 1 cut(s) 665
BstSLI GKGCMC 2 cut(s) 285, 972
BstUI CGCG 1 cut(s) 563
BstV1I GCAGC 2 cut(s) 192, 826
BstX2I RGATCY 1 cut(s) 1060
BstXI CCANNNNNNTGG 1 cut(s) 1071
BstYI RGATCY 1 cut(s) 1060
BsuRI GGCC 2 cut(s) 614, 1049
BtgI CCRYGG 1 cut(s) 774
BtsCI GGATG 4 cut(s) 606, 710, 1081, 1215
BtsIMutI CAGTG 2 cut(s) 726, 1026
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 5 cut(s) 510, 543, 612, 740, 944
CciI TCATGA 2 cut(s) 307, 694
CfoI GCGC 2 cut(s) 210, 958
Cfr13I GGNCC 2 cut(s) 17, 1047
Csp6I GTAC 2 cut(s) 42, 1334
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviAII CATG 4 cut(s) 101, 308, 647, 695
CviQI GTAC 2 cut(s) 42, 1334
DdeI CTNAG 8 cut(s) 72, 233, 266, 578, 616, 804, 913, 1198
DpnI GATC 5 cut(s) 181, 293, 926, 1062, 1080
DpnII GATC 5 cut(s) 179, 291, 924, 1060, 1078
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Ecl136II GAGCTC 2 cut(s) 66, 1363
Eco130I CCWWGG 1 cut(s) 1083
Eco24I GRGCYC 2 cut(s) 68, 1365
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 17
Eco53kI GAGCTC 2 cut(s) 66, 1363
Eco57I CTGAAG 1 cut(s) 752
EcoICRI GAGCTC 2 cut(s) 66, 1363
EcoRII CCWGG 2 cut(s) 1134, 1343
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 1083
EcoT38I GRGCYC 2 cut(s) 68, 1365
ErhI CCWWGG 1 cut(s) 1083
FaeI CATG 4 cut(s) 104, 311, 650, 698
FaqI GGGAC 1 cut(s) 433
FatI CATG 4 cut(s) 100, 307, 646, 694
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 924
Fnu4HI GCNGC 2 cut(s) 206, 815
FokI GGATG 4 cut(s) 613, 697, 1088, 1222
FriOI GRGCYC 2 cut(s) 68, 1365
Fsp4HI GCNGC 2 cut(s) 206, 815
FspBI CTAG 3 cut(s) 143, 785, 965
FspI TGCGCA 1 cut(s) 209
GlaI GCGC 2 cut(s) 209, 957
GluI GCNGC 2 cut(s) 206, 815
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 2 cut(s) 614, 1049
HapII CCGG 2 cut(s) 973, 1188
HhaI GCGC 2 cut(s) 210, 958
Hin1II CATG 4 cut(s) 104, 311, 650, 698
Hin6I GCGC 2 cut(s) 208, 956
HinP1I GCGC 2 cut(s) 208, 956
HindIII AAGCTT 1 cut(s) 1274
HinfI GANTC 5 cut(s) 125, 394, 574, 932, 1192
HpaII CCGG 2 cut(s) 973, 1188
HphI GGTGA 5 cut(s) 60, 73, 401, 419, 443
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 7 cut(s) 166, 193, 228, 493, 619, 801, 1288
Hpy188III TCNNGA 5 cut(s) 95, 308, 695, 842, 898
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 3 cut(s) 649, 727, 971
HpyCH4III ACNGT 4 cut(s) 122, 358, 721, 1030
HpyCH4IV ACGT 1 cut(s) 1281
HpyCH4V TGCA 8 cut(s) 173, 283, 512, 667, 724, 814, 946, 1240
HpyF10VI GCNNNNNNNGC 1 cut(s) 814
HpyF3I CTNAG 8 cut(s) 72, 233, 266, 578, 616, 804, 913, 1198
HpySE526I ACGT 1 cut(s) 1281
Hsp92II CATG 4 cut(s) 104, 311, 650, 698
HspAI GCGC 2 cut(s) 208, 956
KpnI GGTACC 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 924
Kzo9I GATC 5 cut(s) 179, 291, 924, 1060, 1078
LmnI GCTCC 2 cut(s) 158, 1360
Lsp1109I GCAGC 2 cut(s) 192, 826
LweI GCATC 5 cut(s) 170, 219, 597, 801, 907
MaeI CTAG 3 cut(s) 143, 785, 965
MaeII ACGT 1 cut(s) 1281
MaeIII GTNAC 3 cut(s) 95, 122, 1303
MalI GATC 5 cut(s) 181, 293, 926, 1062, 1080
MboI GATC 5 cut(s) 179, 291, 924, 1060, 1078
MboII GAAGA 8 cut(s) 446, 605, 616, 710, 836, 857, 896, 1148
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 1060
MhlI GDGCHC 4 cut(s) 68, 285, 972, 1365
MlyI GAGTC 3 cut(s) 119, 403, 941
MmeI TCCRAC 2 cut(s) 58, 141
MroXI GAANNNNTTC 2 cut(s) 795, 888
MseI TTAA 1 cut(s) 1372
MslI CAYNNNNRTG 3 cut(s) 59, 699, 915
MspI CCGG 2 cut(s) 973, 1188
MspR9I CCNGG 4 cut(s) 973, 1136, 1189, 1345
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 1240
MvaI CCWGG 2 cut(s) 1136, 1345
MvnI CGCG 1 cut(s) 563
MwoI GCNNNNNNNGC 1 cut(s) 814
NciI CCSGG 2 cut(s) 973, 1189
NdeI CATATG 1 cut(s) 154
NdeII GATC 5 cut(s) 179, 291, 924, 1060, 1078
NlaIII CATG 4 cut(s) 104, 311, 650, 698
NlaIV GGNNCC 4 cut(s) 43, 322, 1024, 1106
NmuCI GTSAC 3 cut(s) 95, 122, 1303
NsbI TGCGCA 1 cut(s) 209
PagI TCATGA 2 cut(s) 307, 694
PctI GAATGC 1 cut(s) 1240
PdmI GAANNNNTTC 2 cut(s) 795, 888
PfeI GAWTC 2 cut(s) 574, 1192
PkrI GCNGC 2 cut(s) 207, 816
PleI GAGTC 3 cut(s) 119, 402, 940
PpsI GAGTC 3 cut(s) 119, 402, 940
Psp124BI GAGCTC 2 cut(s) 68, 1365
Psp6I CCWGG 2 cut(s) 1134, 1343
PspGI CCWGG 2 cut(s) 1134, 1343
PspN4I GGNNCC 4 cut(s) 43, 322, 1024, 1106
PspPI GGNCC 2 cut(s) 17, 1047
PstI CTGCAG 1 cut(s) 669
PsuI RGATCY 1 cut(s) 1060
RsaI GTAC 2 cut(s) 43, 1335
RsaNI GTAC 2 cut(s) 42, 1334
RseI CAYNNNNRTG 3 cut(s) 59, 699, 915
SacI GAGCTC 2 cut(s) 68, 1365
SaqAI TTAA 1 cut(s) 1372
SatI GCNGC 2 cut(s) 206, 815
Sau3AI GATC 5 cut(s) 179, 291, 924, 1060, 1078
Sau96I GGNCC 2 cut(s) 17, 1047
SchI GAGTC 3 cut(s) 119, 403, 941
ScrFI CCNGG 4 cut(s) 973, 1136, 1189, 1345
SduI GDGCHC 4 cut(s) 68, 285, 972, 1365
SfaNI GCATC 5 cut(s) 170, 219, 597, 801, 907
SfcI CTRYAG 1 cut(s) 665
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 3 cut(s) 59, 699, 915
SpeI ACTAGT 1 cut(s) 784
SsiI CCGC 1 cut(s) 561
SspMI CTAG 3 cut(s) 143, 785, 965
SstI GAGCTC 2 cut(s) 68, 1365
StyD4I CCNGG 4 cut(s) 971, 1134, 1187, 1343
StyI CCWWGG 1 cut(s) 1083
TaaI ACNGT 4 cut(s) 122, 358, 721, 1030
TaiI ACGT 1 cut(s) 1284
TaqI TCGA 1 cut(s) 290
TfiI GAWTC 2 cut(s) 574, 1192
Tru1I TTAA 1 cut(s) 1372
Tru9I TTAA 1 cut(s) 1372
TscAI CASTG 2 cut(s) 726, 1033
TseFI GTSAC 3 cut(s) 95, 122, 1303
TseI GCWGC 2 cut(s) 205, 814
Tsp45I GTSAC 3 cut(s) 95, 122, 1303
TspDTI ATGAA 7 cut(s) 95, 336, 617, 663, 711, 881, 1221
TspGWI ACGGA 1 cut(s) 613
TspRI CASTG 2 cut(s) 726, 1033
VneI GTGCAC 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 3 cut(s) 1088, 1145, 1292
XmnI GAANNNNTTC 2 cut(s) 795, 888
XspI CTAG 3 cut(s) 143, 785, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.