Rh4BG225100

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000490
Physical Location & Seq
Reverse (-)
38874099 .. 38878499
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG225100.1

Sequence Viewer

Length: 984 bp
ATGTGGGATGCTAGTAATGAAAAACATGGAGATCTCAAAGCAGCTCTCACCATCTTTGTCGTCATCATGACAGCTATTGGTTTCTGGTTCCTCCAGACTTGGAATAAGCACTCGAGGAACCCGAAACAAGCTACATTGCCGCCTGGCCCTCGTGGTGTTCCACTACTCGGTTACCTTCCATTTTTCGGTACGAACCATCACCACCTGTTGACAGACTTGTCAAGGGTTTATGGCCCAATTTACAGGATCCAACTTGGTACCAAACTAGGCATTGTGGTCAGCTCACCATCGTTAGTGAAAGAAGTGGTTCGTGACAAAGACACAATATTTTTCAACCGCGACCTTACAGTGGCACAACAAATTCTCTCAAATGGAGGATCCGATATAATCTTTGGGACTGACGTTCCAAAGTGGAGGAAGATGCGCAAGATGCTCGTGAGTCAAATACTAAGCAAAACCAACCTTAACGATTGTTATGCTCTGAGAAAACAAGAGGTTGTGAAGTCAATTAGGCATGTTTATAATGAAAAAATTGGGACCCCAATTGATTTTGGGCAGTTTGCAATGTCCACAACCTTCAACACATTCTTGCGCATGTTATGGGGTGGGACTTTACAAGAAGAGAAGGCATTTGATGTTGGATCTGAGTTTAGAAAGGTAGCGGGGGAAACCCTCTGGTTACTTCAGAAAGGAAACTTCTCGGACTTTTTCCCTGTGCTTGCTAGGTTTGATATACAGGGAATTAGGAGCCGAGCAAAGAAGCTTTCGTCAGTAACTGAAAACATGCTCAATTCGATCATAGAAACTCAGATGAATAAGGATCAAAATGAGGGAGTACCAAAAAAAGATGAAGGGAAGGGCTTTCTGCAGCTCCTCTTGGAGAATACTATAAATCAAGATAGTGCAACATCACTTACAATGAAACAAGTGAAGGCCTTGCTCTCGATGATGCACATCTTGAGTGCAGGTTCTGTTAGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

327

Amino Acids

36.87

Weight (kDa)

9.68

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 316 4.4e-27 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000490)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10240 FvH4_5g00571 FvH4_5g00571 FvH4_5g00571 FvH4_5g00572 FvH4_5g00572 FvH4_5g00572 FvH4_5g00573 FvH4_5g37440 FvH4_5g37450
prunus_persica Prupe.5G078400_v2.0.a1 Prupe.5G078500_v2.0.a1 Prupe.5G078600_v2.0.a1 Prupe.5G078800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0419931 RchiOBHm_Chr4g0419951 RchiOBHm_Chr4g0420051 RchiOBHm_Chr7g0201681 RchiOBHm_Chr7g0201771 RchiOBHm_Chr7g0201781 RchiOBHm_Chr7g0201791 RchiOBHm_Chr7g0225701 RchiOBHm_Chr7g0225731 RchiOBHm_Chr7g0225761 RchiOBHm_Chr7g0225791 RchiOBHm_Chr7g0225861
rosa_laevigata RLG00000001786 RLG00000001795 RLG00000003649 RLG00000003650 RLG00000003651 RLG00000003652 RLG00000003655 RLG00000003656 RLG00000003657
rosa_multiflora Rmu_sc0001030.1_g000001 Rmu_sc0001030.1_g000005 Rmu_sc0001030.1_g000006 Rmu_sc0006133.1_g000002 Rmu_sc0006637.1_g000005 Rmu_sc0012416.1_g000003 Rmu_sc0016902.1_g000001 Rmu_sc0025481.1_g000003
rosa_roxburghii Rroxscaffold_3G00233490 Rroxscaffold_3G00233550 Rroxscaffold_3G00233560 Rroxscaffold_3G00255100 Rroxscaffold_3G00255110
rosa_rugosa Rorug07G0065300 Rorug07G0065400 Rorug07G0065500 Rorug07G0065600 Rorug07G0065600 Rorug07G0065600 Rorug07G0065700 Rorug07G0065800 Rorug07G0065900 Rorug07G0232900
rosa_samantha Rh3AG071400 Rh3BG286400 Rh3CG072700 Rh4BG225100 Rh4CG235600 Rh5CG585000 Rh7AG192600 Rh7AG193000 Rh7AG193100 Rh7AG193300 Rh7AG371100 Rh7AG387700 Rh7BG194700 Rh7BG194900 Rh7BG195000 Rh7BG366600 Rh7CG203800 Rh7CG204000 Rh7CG204300 Rh7CG389700 Rh7CG407300 Rh7DG198100 Rh7DG198900 Rh7DG199100 Rh7DG377600
rosa_wichuraiana Rw0G020990 Rw7G016880 Rw7G016890 Rw7G028030 Rw7G031840 Rw7G031890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AasI GACNNNNNNGTC 1 cut(s) 217
Acc16I TGCGCA 2 cut(s) 425, 593
Acc36I ACCTGC 1 cut(s) 956
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 257
AccII CGCG 1 cut(s) 339
AciI CCGC 3 cut(s) 140, 337, 662
AclWI GGATC 6 cut(s) 241, 254, 372, 385, 649, 828
AcsI RAATTY 1 cut(s) 360
AcuI CTGAAG 1 cut(s) 668
AfaI GTAC 3 cut(s) 190, 259, 837
AfiI CCNNNNNNNGG 3 cut(s) 167, 185, 349
AgsI TTSAA 2 cut(s) 334, 580
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 6 cut(s) 44, 74, 131, 282, 763, 871
AluI AGCT 6 cut(s) 44, 74, 131, 282, 763, 871
AlwI GGATC 6 cut(s) 241, 254, 372, 385, 649, 828
AlwNI CAGNNNCTG 2 cut(s) 776, 971
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 3 cut(s) 145, 232, 933
ApeKI GCWGC 2 cut(s) 41, 868
ApoI RAATTY 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 257
AspLEI GCGC 2 cut(s) 426, 594
AspS9I GGNCC 3 cut(s) 146, 233, 537
AsuHPI GGTGA 3 cut(s) 40, 191, 276
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 537
BamHI GGATCC 2 cut(s) 246, 377
BanI GGYRCC 1 cut(s) 257
BauI CACGAG 2 cut(s) 150, 434
BbvI GCAGC 2 cut(s) 53, 880
BccI CCATC 3 cut(s) 59, 204, 295
BcgI CGANNNNNNTGC 4 cut(s) 415, 449, 458, 492
BciT130I CCWGG 1 cut(s) 144
BfaI CTAG 3 cut(s) 12, 266, 723
BfmI CTRYAG 1 cut(s) 866
BfuAI ACCTGC 1 cut(s) 956
BglII AGATCT 1 cut(s) 31
BisI GCNGC 3 cut(s) 42, 140, 869
BlsI GCNGC 3 cut(s) 43, 141, 870
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 3 cut(s) 146, 233, 537
BmiI GGNNCC 8 cut(s) 89, 119, 248, 259, 379, 538, 539, 749
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 3 cut(s) 411, 420, 939
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 979
BsaBI GATNNNNATC 1 cut(s) 953
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 185, 349
Bse3DI GCAATG 2 cut(s) 134, 570
Bse8I GATNNNNATC 1 cut(s) 953
BseBI CCWGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 13
BseJI GATNNNNATC 1 cut(s) 953
BseLI CCNNNNNNNGG 3 cut(s) 167, 185, 349
BseMI GCAATG 2 cut(s) 134, 570
BseMII CTCAG 3 cut(s) 473, 636, 821
BseRI GAGGAG 1 cut(s) 863
BseXI GCAGC 2 cut(s) 53, 880
BsgI GTGCAG 1 cut(s) 984
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 147, 234, 935
BshNI GGYRCC 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 112
BslFI GGGAC 3 cut(s) 409, 550, 622
BslI CCNNNNNNNGG 3 cut(s) 167, 185, 349
BsmFI GGGAC 3 cut(s) 409, 550, 622
BsnI GGCC 3 cut(s) 147, 234, 935
BsoBI CYCGRG 1 cut(s) 112
Bsp143I GATC 6 cut(s) 31, 246, 377, 641, 795, 820
BspACI CCGC 3 cut(s) 140, 337, 662
BspANI GGCC 3 cut(s) 147, 234, 935
BspCNI CTCAG 3 cut(s) 474, 637, 820
BspFNI CGCG 1 cut(s) 339
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 8 cut(s) 89, 119, 248, 259, 379, 538, 539, 749
BspMAI CTGCAG 1 cut(s) 870
BspMI ACCTGC 1 cut(s) 956
BspPI GGATC 6 cut(s) 241, 254, 372, 385, 649, 828
BspT107I GGYRCC 1 cut(s) 257
BsrDI GCAATG 2 cut(s) 134, 570
BssMI GATC 6 cut(s) 31, 246, 377, 641, 795, 820
BssSI CACGAG 2 cut(s) 150, 434
Bst2BI CACGAG 2 cut(s) 150, 434
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 615
BstC8I GCNNGC 1 cut(s) 720
BstDEI CTNAG 4 cut(s) 449, 482, 645, 807
BstEII GGTNACC 1 cut(s) 170
BstF5I GGATG 1 cut(s) 13
BstFNI CGCG 1 cut(s) 339
BstHHI GCGC 2 cut(s) 426, 594
BstKTI GATC 6 cut(s) 34, 249, 380, 644, 798, 823
BstMBI GATC 6 cut(s) 31, 246, 377, 641, 795, 820
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 3 cut(s) 518, 598, 787
BstPI GGTNACC 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 866
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 53, 880
BstX2I RGATCY 4 cut(s) 31, 246, 377, 641
BstYI RGATCY 4 cut(s) 31, 246, 377, 641
BsuRI GGCC 3 cut(s) 147, 234, 935
BtsCI GGATG 1 cut(s) 13
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 956
Cac8I GCNNGC 1 cut(s) 720
CaiI CAGNNNCTG 2 cut(s) 776, 971
CciI TCATGA 1 cut(s) 66
CfoI GCGC 2 cut(s) 426, 594
Cfr13I GGNCC 3 cut(s) 146, 233, 537
Csp6I GTAC 3 cut(s) 189, 258, 836
CviAII CATG 5 cut(s) 26, 67, 515, 595, 784
CviQI GTAC 3 cut(s) 189, 258, 836
DdeI CTNAG 4 cut(s) 449, 482, 645, 807
DpnI GATC 6 cut(s) 33, 248, 379, 643, 797, 822
DpnII GATC 6 cut(s) 31, 246, 377, 641, 795, 820
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco147I AGGCCT 1 cut(s) 935
Eco47I GGWCC 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 537
EcoO65I GGTNACC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 142
FaeI CATG 5 cut(s) 29, 70, 518, 598, 787
FaqI GGGAC 3 cut(s) 409, 550, 622
FatI CATG 5 cut(s) 25, 66, 514, 594, 783
FauI CCCGC 1 cut(s) 655
Fnu4HI GCNGC 3 cut(s) 42, 140, 869
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 42, 140, 869
FspBI CTAG 3 cut(s) 12, 266, 723
FspI TGCGCA 2 cut(s) 425, 593
GlaI GCGC 2 cut(s) 425, 593
GluI GCNGC 3 cut(s) 42, 140, 869
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 3 cut(s) 147, 234, 935
HhaI GCGC 2 cut(s) 426, 594
Hin1II CATG 5 cut(s) 29, 70, 518, 598, 787
Hin6I GCGC 2 cut(s) 424, 592
HinP1I GCGC 2 cut(s) 424, 592
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 1 cut(s) 439
HphI GGTGA 3 cut(s) 40, 191, 276
Hpy166II GTNNAC 2 cut(s) 210, 570
Hpy188I TCNGA 6 cut(s) 382, 483, 646, 687, 703, 810
Hpy188III TCNNGA 7 cut(s) 67, 94, 311, 436, 896, 943, 958
Hpy8I GTNNAC 2 cut(s) 210, 570
HpyAV CCTTC 6 cut(s) 185, 586, 619, 845, 850, 925
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 563, 868, 905, 952, 965
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 4 cut(s) 449, 482, 645, 807
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 5 cut(s) 29, 70, 518, 598, 787
HspAI GCGC 2 cut(s) 424, 592
KflI GGGWCCC 1 cut(s) 537
KpnI GGTACC 1 cut(s) 261
Kzo9I GATC 6 cut(s) 31, 246, 377, 641, 795, 820
LmnI GCTCC 2 cut(s) 747, 876
Lsp1109I GCAGC 2 cut(s) 53, 880
LweI GCATC 3 cut(s) 411, 420, 939
MaeI CTAG 3 cut(s) 12, 266, 723
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 4 cut(s) 170, 311, 678, 772
MalI GATC 6 cut(s) 33, 248, 379, 643, 797, 822
MboI GATC 6 cut(s) 31, 246, 377, 641, 795, 820
MboII GAAGA 2 cut(s) 430, 632
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 4 cut(s) 31, 246, 377, 641
MluCI AATT 7 cut(s) 237, 360, 507, 531, 543, 741, 790
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 2 cut(s) 274, 619
MnlI CCTC 9 cut(s) 101, 108, 159, 368, 408, 487, 683, 823, 884
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 1 cut(s) 465
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 430
NdeII GATC 6 cut(s) 31, 246, 377, 641, 795, 820
NlaIII CATG 5 cut(s) 29, 70, 518, 598, 787
NlaIV GGNNCC 8 cut(s) 89, 119, 248, 259, 379, 538, 539, 749
NmeAIII GCCGAG 1 cut(s) 776
NmuCI GTSAC 1 cut(s) 311
NsbI TGCGCA 2 cut(s) 425, 593
NspI RCATGY 3 cut(s) 518, 598, 787
PaeR7I CTCGAG 1 cut(s) 112
PagI TCATGA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 306
PkrI GCNGC 3 cut(s) 43, 141, 870
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
PpuMI RGGWCCY 1 cut(s) 537
PsiI TTATAA 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 8 cut(s) 89, 119, 248, 259, 379, 538, 539, 749
PspPI GGNCC 3 cut(s) 146, 233, 537
PspPPI RGGWCCY 1 cut(s) 537
PspXI VCTCGAGB 1 cut(s) 112
PstI CTGCAG 1 cut(s) 870
PstNI CAGNNNCTG 2 cut(s) 776, 971
PsuI RGATCY 4 cut(s) 31, 246, 377, 641
RsaI GTAC 3 cut(s) 190, 259, 837
RsaNI GTAC 3 cut(s) 189, 258, 836
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 3 cut(s) 42, 140, 869
Sau3AI GATC 6 cut(s) 31, 246, 377, 641, 795, 820
Sau96I GGNCC 3 cut(s) 146, 233, 537
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 144
SfaNI GCATC 3 cut(s) 411, 420, 939
SfcI CTRYAG 1 cut(s) 866
Sfr274I CTCGAG 1 cut(s) 112
SinI GGWCC 1 cut(s) 537
SlaI CTCGAG 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 112, 958
SmoI CTYRAG 2 cut(s) 112, 958
Sse9I AATT 7 cut(s) 237, 360, 507, 531, 543, 741, 790
SseBI AGGCCT 1 cut(s) 935
SsiI CCGC 3 cut(s) 140, 337, 662
SspI AATATT 1 cut(s) 327
SspMI CTAG 3 cut(s) 12, 266, 723
StuI AGGCCT 1 cut(s) 935
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 113, 794, 944
TasI AATT 7 cut(s) 237, 360, 507, 531, 543, 741, 790
TauI GCSGC 1 cut(s) 142
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 41, 868
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 5 cut(s) 33, 540, 827, 864, 935
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 360
XceI RCATGY 3 cut(s) 518, 598, 787
XhoI CTCGAG 1 cut(s) 112
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 12, 266, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.