Rorug01G0249500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000506
Physical Location & Seq
Forward (+)
35772224 .. 35781739
9516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0249500.1

Sequence Viewer

Length: 5322 bp
ATGGCTTCTTCGGAAGCCGATTCCCGTCTCCGACAGGTCGTTTCTCCCGCCCTAGAGAAGATCATCAAGAACGCCTCATGGCGCAAGCACGCCAAGCTCGCCAACGAATGCAAGGCCGTCATCGAGCGCCTCTCCAACCCGGACAAGGCGGCCCAGCCCGCCGGCCCGGATTCCGAACCCGACGCCTCCGAGCCCGGCCCGCTCCACGACGGAGGCTCCGGTGAGTACTCCCTCGCCGACGCCGAGTCGATTCTCAGCCCTCTCATCGGCGCCGCCGCATCGGGAGTGCTGAAGATCGCCGATCCCGCCGTCGACTGCATCCAGAAGCTCATCGCGCATGGCTACTTGCGCGGCGAGGCCGACCCCTCCGGTGGCGCCGAGGCGAAGTTGCTGACGAAATTGATCGAGTCCGTTTGTAAATGCCACGATTTGGGGGACGATCAGATGGAGCTGCTGGTGCTGAAGACGCTGCTGTCAGCAGTGACGTCGATTTCGCTGCGGATTCACGGCGATTGCTTGCTTCAGATAGTGAGGACTTGCTATGATATCTATTTGGGGAGTAAGAACATAGTGAATCAGACGACGGCGAAGGCCTCGCTGATCCAGATGCTGGTGATTGTGTTCCGGCGAATGGAGGCGGACTCTTCTACGGTGCCGATTAATCCCATTGTGGTGGCGGAATTGATGGATCCCATTGAGAAATCTGATGCTGATGGGTCCATGACTATGTTCGTACAAGGGTTTATTACCAAAATTATGTCTGACATTGATGGGGTGTTGAATCCGACGACGCCTACTAAGCTTTCCGGGCATGATGGGGCGTTTGAGACCACCACGGTGGAGACCACGAATCCGGCGGACTTGCTGGACTCGACTGATAAGGATATGTTGGATGCCAAGTACTGGGAGATTAGTATGTACAAGACGGCATTGGAGGGCCGGAAGGGAGAGCTGGCGGATGGGGAAGTAGAGAGAGATGAAGATTTGGAGGTTCAGATTGGCAATAAGTTGCGCAGAGATGCTTTTTTGGTGTTTAGAGCTCTTTGTAAGCTGTCTATGAAGACCCCGCCCAAGGAGGCATTGGCGGATCCGGAGTTGATGAAGGGAAAGATTGTGGCATTGGAGCTTTTGAAGATTTTGTTGGAGAATGCTGGTGCTGTGTTTAGGACCAGCGACAGGTTTTTAGGTGCAATTAAGCAATACTTATGTCTGTCATTGTTAAAGAACAGCGCGTCAACTCTTATGATAGTTTTTCAGCTATCTTGTTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTCTTTCCTATGATTGTTCTCAGAGTTTTAGAGAATGTTGCACAACCTAATTTTCAGCAGAAGATGATAGTGCTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTCGACATATTTATCAATTATGATTGCGATGTCAGTTCATCAAACATATTTGAGAGGATGGTAAATGGCCTTCTTAAAACTGCCCAAGGTGTCCCTCCTGGTGTTGCCACCACGTTATTACCTCCTCAAGAGGCAAATATGAAACTTGAAGCCATGAGGTGTTTAGTTGCTATTCTGAGGTCAATTGGGGACTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTACCAAGAAATCTGAAGTAAATGAGAATAGTTCTGAACCAGGAGCTCTTACCATGGCAAATGGGAATGGTGAGGAGTCTGTTGAAGGATCAGATTCTCAATCTGAAGCCTCCAGTGAAGCCTCTGATGCTTTGACAATCGAGCAACGCCGAGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAGAAAGGAATTGAGTTTCTTATCAATGCAAATAAGGTGGGCAATTCACCCGAGGAGATAGCTGCTTTTCTAAAAAATGCATCTGGTTTGAACAAGACTTTGATTGGAGACTACCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGATTTTCAAGACTTGGAGTTTGATGAGGCAATCAGGTCCTTTCTTCAAGGCTTTAGGTTGCCTGGTGAGGCACAGAAGATTGACAGAATAATGGAAAAGTTTGCTGAGCGCTATTGCAAGTGCAATCCAAAGGCTTTTACTAGTGCTGATACAGCCTATGTCCTTGCTTACTCCGTGATACTGCTCAATACTGATGCTCATAACCCGATGGTGAAGAGCAAGATGTCAGCTGATGATTTTATAAGAAACAATCGTGGCATAGATGATGGAAAAGATCTACCTGAGCAGTACTTGAGGTCCTTGTATGAACGAATATCAAGAAAGGAGATAAAAATGAAAGAATATGATTTGGCTCCTCAGCAGATACAGTCGGTGAACCCTAACCGTCTTTTGGGCTTGGATAGTATCTTGAATATTGTGATCCGTAAACGGGGGGATAGTCAATTGGAGACCAGTGATGATCTTATCAAGCATATGCAAGAGCAATTCCAAGAAAAAGCTCGGAAATCTGAGTCAGTTTACTATGCTGCAACAGATGTGGTAATTCTTAGATTCATGGTTGAAGTCTGCTGGGCTCCTATGCTGGCTGCCTTCAGTGTTCCACTTGACCAAACTGATGATGAAGTAGTTATTTCTCTGTGTCTTGAAGGGATTCGTTATGCTATCCATGTAACTGCCGCAATGTCCATGAAGACCCATAGAGATGCTTTTGTGACTTCACTGGCGAAGTTTACTTCCCTCCACTCTCCTGCGGATATCAAGCAGAAAAACATAGAAGCAATCAAGGCAATAGTGACAATTGCAGATGAAGATGGGAACTACCTGCAAGAAGCTTGGGAGCATATTTTGACATGTGTTTCCCGCTTTGAACATCTTCATCTCCTGGGTGAGGGTGCTCCTCCAGATGCTACTTTCTTTGCTTTTCCTCAGAATGAATCAGAAAAATCAAAGCAAGCCAAGTCAACTGTCCTTCCTGTTTTAAAAAAGAAGGGCCAAGGGAAGATGCAGTATGCTGCTGCTGCCGTGTTGAGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATTCTTCTGGGATGGTCACATCAGAACAGATGAACAATTTGGTTTCTAACTTAAATATGTTAGAACAAGTTGGAGACATGAGCCGCATATTCACACGGAGTCAAAAGCTGAACAGTGAGGCAATTATAGATTTTGTTAAGGCTCTTTGCAAGGTCTCTATGGAGGAATTGCGATCTGCATCTGATCCTCGGGTTTTCAGCCTTACGAAGATGGTTGAGATTGCGCATTATAACATGAACCGCATCAGGCTTGTGTGGTCTAGCATCTGGCATGTACTCTCTAATTTCTTCGTGACAATTGGCTGTTCTGAAAACCTGTCTATTGCAATTTTTGCTATGGATTCTCTACGCCAATTGTCAATGAAATTCTTGGAGCGAGAAGAGCTGGCTAATTATAATTTTCAGAATGAGTTTATGAAACCTTTTGTCATTGTTATGCGTAAAAGTAGTGCAGTTGAGATACGAGAATTAATCATCAGATGTGTCTCCCAAATGGTTTTATCTCGTGTGAATAGAGTCAAATCTGGATGGAAGAGCATGTTCATGGTCTTCACTACGGCAGCTTATGATGACCACAAAAATATTGTACTCTTGGCCTTTGAAATAATTGAGAAGATTATTCGCGACTACTTTCCGTACATCACCGAGACTGAAACCACTACTTTTACTGATTGCGTAAATTGTCTAATTGCATTCACCAATAGTAGATTTAACAAAGACATTAGCCTCAATGCTATTGCTTTTCTTCGGTTCTGTGCAACAAAACTGGCTGAGGGAGGTCTTAGTTCCTCATCAAGAAATAAGGACAAGGATGCATCTGGAAAAATATCTCCAACGTCACCTCAAGGATGGAAAGAAGGAAGACAAGATAATGGGGGAATGCCAGACAAGGATGACCATCTTTATTTCTGGTTCCCTTTATTGGCTGGTTTGTCCGAACTTAGCTTTGATCCTAGGCCTGAAATCAGGAAGAGTGCCTTACAAGTGCTGTTTGAAACTCTACGTAACCATGGTCACCTTTTCTCTCTACCTTTGTGGGAAAAGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGCCATGCTATTGATCCTTCTGGGGAAAGTTCACCAGAGCAAGGGATTGACAGTGAAACAGGCGAGCTTGATCAAGATGCCTGGATGTACGAGACATGCACATTGGCACTCCAATTAGTTGTTGATCTTTTTGTCAAGTTTTATGACACTGTCAATCCTCTTTTGAAGAAAGTTTTGGATCTTCTAGTCAGTTTTATTGGACGACCTCACCAAAGCCTAGCTGGCATAGGTATTGCTGCATTTGTTCGATTGATGAGCAATGCAGGGGATCTCTTTTCTGATGAGAAGTGGCTTGAAGTGGTTTCATCATTAAAAGAAGCTGCCAATTCAACTCTTCCTGATTTTTCTTTCATTCTTAACGGAGATAGCATCATGGGAATCCTAGACTCTTCTTCTAGTAGAGAAGATCATGGAGGGTCTGGGAGGCCTGATGATGATTCAGAGAGACTAAGGACAAACCATCTTTATGCTGGCTTGGCTGACGTTAAGTGCCGAGCTGCTGTTCAGCTATTATTGATTCAGGCAGTGATGGAGATTTACACCATGTATCGTTCTCACCTTTCATCGAATAACACATTAATCCTCTTCAATGCTCTGCATGATTTGGCATCCCATGCTCACAAAGTTAACACTGATACCACTTTACGGGCAAGGCTACAAGAGTTTGGCTCCATGACGCAAATGCAAGACCCTCCACTGTTACGTATTGAGAACGAATCTTACCAAATCTGCCTTACATTCTTACAGAATCTTGTAGAGGACAGGCCTCCAAGTTATGATGAGGTGGAGGTAGAGTCCCACATTGTGGACCTTTGCAAGGAGGTTTTACAGTTCTATATTGAAGCCGCAAGCTCTGGGAAGATATCTGAATCATCTAATGGGCAGCAGCACCATTGGCTGATCCCTCTAGGTTCTGGGCGACGGAGAGAATTGGCTGCACGTGCCCCGCTTATTGTGGCGACTCTCCAGGCCATCTGTTGCTTGGGAGACACTTCATTTGAAAATAACTTGTCTCATTTCTTCCCGCTGCTTGCAACCTTGATAACTTGTGAACATGGGTCCGATGAGGTCCAGGCTGCCCTTAGTGACATGCTCAGCTCATCTGTGGGTCCTGTTTTGCTTCGATCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1773

Amino Acids

197.18

Weight (kDa)

5.32

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 6 - 214 1.2e-37 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 338 - 496 2.6e-47 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 610 - 791 6.1e-78 Sec7 domain
Sec7-like_HDS PF09324 1157 - 1239 1.7e-29 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1329 - 1417 2.6e-12 C-terminal region of Mon2 protein
BIG2_C PF20252 1563 - 1762 4.9e-43 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45990 AT2G45990 AT2G45990 AT2G45990
fragaria_vesca FvH4_3g44250 FvH4_3g44250 FvH4_3g44250 FvH4_3g44250 FvH4_3g44250 FvH4_7g14540 FvH4_7g14540 FvH4_7g14540 FvH4_7g14540 FvH4_7g14540 FvH4_7g14540 FvH4_7g14540 FvH4_7g14540 FvH4_7g14571 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620 FvH4_7g14620
malus_domestica MD01G1065200.v1.1 MD09G1245400.v1.1
prunus_persica Prupe.2G172200_v2.0.a1
pyrus_communis pycom01g09450
rosa_chinensis RchiOBHm_Chr1g0355961 RchiOBHm_Chr1g0356011 RchiOBHm_Chr1g0356041 RchiOBHm_Chr1g0356051 RchiOBHm_Chr1g0356101 RchiOBHm_Chr1g0356141
rosa_laevigata RLG00000028102 RLG00000028107 RLG00000028108 RLG00000028110 RLG00000028112
rosa_multiflora Rmu_sc0002569.1_g000001 Rmu_sc0002569.1_g000022 Rmu_sc0003229.1_g000002 Rmu_sc0004705.1_g000007 Rmu_sc0007127.1_g000003 Rmu_sc0007127.1_g000007 Rmu_sc0011003.1_g000003 Rmu_sc0014206.1_g000012
rosa_roxburghii Rroxscaffold_4G00299780 Rroxscaffold_4G00299790 Rroxscaffold_4G00299810 Rroxscaffold_4G00299820 Rroxscaffold_4G00299870 Rroxscaffold_4G00299890
rosa_rugosa Rorug01G0249000 Rorug01G0249200 Rorug01G0249300 Rorug01G0249400 Rorug01G0249400 Rorug01G0249500 Rorug01G0249600
rosa_samantha Rh1BG230700 Rh1BG230800 Rh1BG230900 Rh1BG231200 Rh1BG231300 Rh1CG041500 Rh1CG244900 Rh1CG245500 Rh1CG245600 Rh1CG246100 Rh1CG246200 Rh1DG028400 Rh1DG258800 Rh1DG258900 Rh1DG259000
rosa_wichuraiana Rw1G023130 Rw1G023160 Rw1G023170 Rw1G023180 Rw1G023220 Rw1G023230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2285, 3336, 3501
AasI GACNNNNNNGTC 1 cut(s) 472
AatII GACGTC 1 cut(s) 488
Acc16I TGCGCA 3 cut(s) 1013, 1652, 3330
Acc36I ACCTGC 1 cut(s) 2874
AccB1I GGYRCC 3 cut(s) 269, 374, 652
AccB7I CCANNNNNTGG 5 cut(s) 430, 610, 903, 1438, 4993
AccBSI CCGCTC 1 cut(s) 202
AccI GTMKAC 2 cut(s) 312, 1443
AccII CGCG 4 cut(s) 335, 351, 1232, 3729
AccIII TCCGGA 1 cut(s) 1090
AcsI RAATTY 2 cut(s) 3106, 3470
AcuI CTGAAG 7 cut(s) 311, 482, 506, 1704, 1794, 1823, 2620
AcvI CACGTG 1 cut(s) 5129
AcyI GRCGYC 6 cut(s) 183, 240, 270, 375, 485, 791
AdeI CACNNNGTG 1 cut(s) 4993
AfaI GTAC 9 cut(s) 227, 735, 902, 920, 2333, 3381, 3693, 3743, 4277
AfeI AGCGCT 1 cut(s) 2153
AflIII ACRYGT 1 cut(s) 2894
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 2183
AjnI CCWGG 7 cut(s) 1537, 1708, 2104, 2925, 4268, 5154, 5259
AjuI GAANNNNNNNTTGG 4 cut(s) 1124, 1156, 4346, 4378
AleI CACNNNNGTG 1 cut(s) 836
Alw21I GWGCWC 3 cut(s) 1042, 1717, 2941
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 2 cut(s) 1922, 3294
Aor13HI TCCGGA 1 cut(s) 1090
Aor51HI AGCGCT 1 cut(s) 2153
ApoI RAATTY 2 cut(s) 3106, 3470
ArsI GACNNNNNNTTYG 6 cut(s) 475, 507, 2045, 2077, 5168, 5200
AseI ATTAAT 3 cut(s) 660, 3575, 4766
Asp700I GAANNNNTTC 2 cut(s) 2694, 2916
AspA2I CCTAGG 1 cut(s) 4058
AsuC2I CCSGG 4 cut(s) 140, 167, 195, 808
AvaI CYCGRG 2 cut(s) 1922, 3294
AvaII GGWCC 8 cut(s) 717, 1167, 2079, 2340, 4996, 5247, 5257, 5297
AvrII CCTAGG 1 cut(s) 4058
BaeGI GKGCMC 1 cut(s) 5134
BamHI GGATCC 2 cut(s) 688, 1087
BanI GGYRCC 3 cut(s) 269, 374, 652
BanII GRGCYC 4 cut(s) 195, 1042, 1717, 2620
BarI GAAGNNNNNNTAC 2 cut(s) 1494, 1526
BauI CACGAG 1 cut(s) 3609
BbrPI CACGTG 1 cut(s) 5129
BbsI GAAGAC 6 cut(s) 470, 1067, 2007, 2741, 3646, 3973
Bbv12I GWGCWC 3 cut(s) 1042, 1717, 2941
BbvCI CCTCAGC 2 cut(s) 2400, 3876
BceAI ACGGC 7 cut(s) 101, 293, 523, 600, 942, 3050, 3678
BcgI CGANNNNNNTGC 4 cut(s) 468, 478, 502, 512
BciT130I CCWGG 7 cut(s) 1539, 1710, 2106, 2927, 4270, 5156, 5261
BclI TGATCA 1 cut(s) 4258
BcnI CCSGG 4 cut(s) 140, 167, 195, 808
BcuI ACTAGT 1 cut(s) 2183
BfaI CTAG 9 cut(s) 53, 2184, 3366, 4059, 4373, 4406, 4571, 4584, 5096
BfoI RGCGCY 4 cut(s) 130, 273, 378, 2155
BfuAI ACCTGC 1 cut(s) 2874
BglI GCCNNNNNGGC 1 cut(s) 4410
BglII AGATCT 2 cut(s) 1434, 2317
BlnI CCTAGG 1 cut(s) 4058
BlpI GCTNAGC 2 cut(s) 2148, 5282
BmcAI AGTACT 3 cut(s) 227, 902, 2333
Bme18I GGWCC 8 cut(s) 717, 1167, 2079, 2340, 4996, 5247, 5257, 5297
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 2 cut(s) 1922, 3294
BmrI ACTGGG 1 cut(s) 913
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 6 cut(s) 470, 1067, 2007, 2741, 3646, 3973
BplI GAGNNNNNCTC 6 cut(s) 116, 148, 626, 658, 4841, 4873
BpmI CTGGAG 3 cut(s) 1765, 2928, 5138
Bpu10I CCTNAGC 3 cut(s) 2325, 2400, 3876
Bpu1102I GCTNAGC 2 cut(s) 2148, 5282
BpuEI CTTGAG 4 cut(s) 1430, 1551, 2356, 3933
BpuMI CCSGG 4 cut(s) 140, 167, 195, 808
BsaAI YACGTR 3 cut(s) 4109, 4892, 5129
BsaBI GATNNNNATC 2 cut(s) 3283, 4002
BsaHI GRCGYC 6 cut(s) 183, 240, 270, 375, 485, 791
BsaI GGTCTC 4 cut(s) 821, 836, 2486, 3265
BsaWI WCCGGW 3 cut(s) 218, 368, 1090
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 4967, 4997
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 3876
Bse3DI GCAATG 2 cut(s) 2730, 4453
Bse8I GATNNNNATC 2 cut(s) 3283, 4002
BseAI TCCGGA 1 cut(s) 1090
BseBI CCWGG 7 cut(s) 1539, 1710, 2106, 2927, 4270, 5156, 5261
BseJI GATNNNNATC 2 cut(s) 3283, 4002
BseMI GCAATG 2 cut(s) 2730, 4453
BseNI ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 3876
BseRI GAGGAG 5 cut(s) 1554, 1757, 1940, 2388, 2931
BseSI GKGCMC 1 cut(s) 5134
BseYI CCCAGC 2 cut(s) 153, 2613
BsgI GTGCAG 2 cut(s) 3576, 5109
Bsh1236I CGCG 4 cut(s) 335, 351, 1232, 3729
BshNI GGYRCC 3 cut(s) 269, 374, 652
BsiHKAI GWGCWC 3 cut(s) 1042, 1717, 2941
BsiHKCI CYCGRG 2 cut(s) 1922, 3294
BslFI GGGAC 4 cut(s) 449, 1517, 1643, 4969
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 4 cut(s) 449, 1517, 1643, 4969
BsmI GAATGC 5 cut(s) 113, 1153, 1653, 3797, 3990
Bso31I GGTCTC 4 cut(s) 821, 836, 2486, 3265
BsoBI CYCGRG 2 cut(s) 1922, 3294
Bsp1286I GDGCHC 6 cut(s) 195, 1042, 1717, 2620, 2941, 5134
Bsp13I TCCGGA 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 918
Bsp1720I GCTNAGC 2 cut(s) 2148, 5282
Bsp19I CCATGG 2 cut(s) 1722, 4114
Bsp68I TCGCGA 1 cut(s) 3729
BspEI TCCGGA 1 cut(s) 1090
BspFNI CGCG 4 cut(s) 335, 351, 1232, 3729
BspMI ACCTGC 1 cut(s) 2874
BspQI GCTCTTC 3 cut(s) 2252, 3480, 3632
BspT107I GGYRCC 3 cut(s) 269, 374, 652
BspTNI GGTCTC 4 cut(s) 821, 836, 2486, 3265
BsrBI CCGCTC 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 2730, 4453
BsrFI RCCGGY 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 3876
BssAI RCCGGY 1 cut(s) 161
BssNI GRCGYC 6 cut(s) 183, 240, 270, 375, 485, 791
BssSI CACGAG 1 cut(s) 3609
BssT1I CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
Bst2BI CACGAG 1 cut(s) 3609
Bst2UI CCWGG 7 cut(s) 1539, 1710, 2106, 2927, 4270, 5156, 5261
Bst6I CTCTTC 8 cut(s) 649, 2252, 3480, 3632, 4070, 4527, 4582, 4778
BstACI GRCGYC 6 cut(s) 183, 240, 270, 375, 485, 791
BstAPI GCANNNNNTGC 2 cut(s) 3437, 4802
BstAUI TGTACA 1 cut(s) 918
BstBAI YACGTR 3 cut(s) 4109, 4892, 5129
BstDSI CCRYGG 3 cut(s) 834, 1722, 4114
BstEII GGTNACC 1 cut(s) 4118
BstENI CCTNNNNNAGG 3 cut(s) 1875, 2930, 5003
BstFNI CGCG 4 cut(s) 335, 351, 1232, 3729
BstH2I RGCGCY 4 cut(s) 130, 273, 378, 2155
BstNI CCWGG 7 cut(s) 1539, 1710, 2106, 2927, 4270, 5156, 5261
BstNSI RCATGY 6 cut(s) 2027, 2898, 3380, 3646, 4287, 5281
BstPI GGTNACC 1 cut(s) 4118
BstSLI GKGCMC 1 cut(s) 5134
BstSNI TACGTA 2 cut(s) 4109, 4892
BstUI CGCG 4 cut(s) 335, 351, 1232, 3729
BstV2I GAAGAC 6 cut(s) 470, 1067, 2007, 2741, 3646, 3973
BstX2I RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
BstXI CCANNNNNNTGG 2 cut(s) 673, 838
BstYI RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
BtgI CCRYGG 3 cut(s) 834, 1722, 4114
BtgZI GCGATG 2 cut(s) 316, 1482
BtsI GCAGTG 2 cut(s) 486, 4719
BtuMI TCGCGA 1 cut(s) 3729
BveI ACCTGC 1 cut(s) 2874
CaiI CAGNNNCTG 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 161
CseI GACGC 6 cut(s) 191, 248, 475, 799, 1221, 4873
Csp6I GTAC 9 cut(s) 226, 734, 901, 919, 2332, 3380, 3692, 3742, 4276
CspCI CAANNNNNGTGG 4 cut(s) 1890, 1925, 2562, 2597
CviQI GTAC 9 cut(s) 226, 734, 901, 919, 2332, 3380, 3692, 3742, 4276
DinI GGCGCC 2 cut(s) 271, 376
DraI TTTAAA 1 cut(s) 3024
DraIII CACNNNGTG 1 cut(s) 4993
DrdI GACNNNNNNGTC 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 8 cut(s) 649, 2252, 3480, 3632, 4070, 4527, 4582, 4778
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 8 cut(s) 649, 2252, 3480, 3632, 4070, 4527, 4582, 4778
EciI GGCGGA 5 cut(s) 653, 692, 872, 971, 1100
Ecl136II GAGCTC 2 cut(s) 1040, 1715
Eco105I TACGTA 2 cut(s) 4109, 4892
Eco130I CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
Eco147I AGGCCT 4 cut(s) 593, 4063, 4615, 4954
Eco24I GRGCYC 4 cut(s) 195, 1042, 1717, 2620
Eco31I GGTCTC 4 cut(s) 821, 836, 2486, 3265
Eco32I GATATC 3 cut(s) 547, 2800, 5052
Eco47I GGWCC 8 cut(s) 717, 1167, 2079, 2340, 4996, 5247, 5257, 5297
Eco47III AGCGCT 1 cut(s) 2153
Eco53kI GAGCTC 2 cut(s) 1040, 1715
Eco57I CTGAAG 7 cut(s) 311, 482, 506, 1704, 1794, 1823, 2620
Eco72I CACGTG 1 cut(s) 5129
Eco88I CYCGRG 2 cut(s) 1922, 3294
Eco91I GGTNACC 1 cut(s) 4118
EcoICRI GAGCTC 2 cut(s) 1040, 1715
EcoNI CCTNNNNNAGG 3 cut(s) 1875, 2930, 5003
EcoO109I RGGNCCY 3 cut(s) 2079, 2340, 5297
EcoO65I GGTNACC 1 cut(s) 4118
EcoRI GAATTC 1 cut(s) 3106
EcoRII CCWGG 7 cut(s) 1537, 1708, 2104, 2925, 4268, 5154, 5259
EcoRV GATATC 3 cut(s) 547, 2800, 5052
EcoT14I CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
EcoT22I ATGCAT 3 cut(s) 1954, 2025, 3922
EcoT38I GRGCYC 4 cut(s) 195, 1042, 1717, 2620
EgeI GGCGCC 2 cut(s) 271, 376
EheI GGCGCC 2 cut(s) 271, 376
ErhI CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
Esp3I CGTCTC 1 cut(s) 32
FalI AAGNNNNNCTT 4 cut(s) 1187, 1219, 4067, 4099
FaqI GGGAC 4 cut(s) 449, 1517, 1643, 4969
FauI CCCGC 8 cut(s) 55, 166, 207, 313, 1074, 2912, 5142, 5220
FauNDI CATATG 2 cut(s) 2517, 3082
FbaI TGATCA 1 cut(s) 4258
FblI GTMKAC 2 cut(s) 312, 1443
FriOI GRGCYC 4 cut(s) 195, 1042, 1717, 2620
FspBI CTAG 9 cut(s) 53, 2184, 3366, 4059, 4373, 4406, 4571, 4584, 5096
FspI TGCGCA 3 cut(s) 1013, 1652, 3330
GsaI CCCAGC 2 cut(s) 157, 2617
GsuI CTGGAG 3 cut(s) 1765, 2928, 5138
HaeII RGCGCY 4 cut(s) 130, 273, 378, 2155
HgaI GACGC 6 cut(s) 191, 248, 475, 799, 1221, 4873
Hin1I GRCGYC 6 cut(s) 183, 240, 270, 375, 485, 791
HincII GTYRAC 5 cut(s) 313, 1236, 1444, 3006, 4816
HindII GTYRAC 5 cut(s) 313, 1236, 1444, 3006, 4816
HindIII AAGCTT 2 cut(s) 800, 2874
HpaI GTTAAC 1 cut(s) 4816
Hpy99I CGWCG 9 cut(s) 185, 212, 242, 314, 490, 586, 790, 793, 5112
HpyCH4IV ACGT 7 cut(s) 485, 1553, 3941, 4108, 4671, 4891, 5128
HpySE526I ACGT 7 cut(s) 485, 1553, 3941, 4108, 4671, 4891, 5128
Hsp92I GRCGYC 6 cut(s) 183, 240, 270, 375, 485, 791
KasI GGCGCC 2 cut(s) 269, 374
Kpn2I TCCGGA 1 cut(s) 1090
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 4258
KspAI GTTAAC 1 cut(s) 4816
LguI GCTCTTC 3 cut(s) 2252, 3480, 3632
MaeI CTAG 9 cut(s) 53, 2184, 3366, 4059, 4373, 4406, 4571, 4584, 5096
MaeII ACGT 7 cut(s) 485, 1553, 3941, 4108, 4671, 4891, 5128
MbiI CCGCTC 1 cut(s) 202
MfeI CAATTG 6 cut(s) 1623, 1646, 2486, 2841, 3402, 3458
MflI RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
MhlI GDGCHC 6 cut(s) 195, 1042, 1717, 2620, 2941, 5134
Mly113I GGCGCC 2 cut(s) 270, 375
MmeI TCCRAC 7 cut(s) 55, 159, 809, 870, 1122, 3157, 3962
Mph1103I ATGCAT 3 cut(s) 1954, 2025, 3922
MroI TCCGGA 1 cut(s) 1090
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 2 cut(s) 2694, 2916
MslI CAYNNNNRTG 8 cut(s) 671, 725, 836, 2028, 2745, 3539, 3647, 4653
MspA1I CMGCKG 2 cut(s) 2273, 5215
MunI CAATTG 6 cut(s) 1623, 1646, 2486, 2841, 3402, 3458
Mva1269I GAATGC 5 cut(s) 113, 1153, 1653, 3797, 3990
MvaI CCWGG 7 cut(s) 1539, 1710, 2106, 2927, 4270, 5156, 5261
MvnI CGCG 4 cut(s) 335, 351, 1232, 3729
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 2 cut(s) 270, 375
NciI CCSGG 4 cut(s) 140, 167, 195, 808
NcoI CCATGG 2 cut(s) 1722, 4114
NdeI CATATG 2 cut(s) 2517, 3082
NgoMIV GCCGGC 1 cut(s) 161
NmeAIII GCCGAG 4 cut(s) 268, 403, 1844, 4706
NmuCI GTSAC 8 cut(s) 481, 2755, 2836, 3121, 3397, 3942, 4118, 5273
NruI TCGCGA 1 cut(s) 3729
NsbI TGCGCA 3 cut(s) 1013, 1652, 3330
NsiI ATGCAT 3 cut(s) 1954, 2025, 3922
NspI RCATGY 6 cut(s) 2027, 2898, 3380, 3646, 4287, 5281
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 2027
PceI AGGCCT 4 cut(s) 593, 4063, 4615, 4954
PciI ACATGT 1 cut(s) 2894
PciSI GCTCTTC 3 cut(s) 2252, 3480, 3632
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 5 cut(s) 113, 1153, 1653, 3797, 3990
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 2694, 2916
PflFI GACNNNGTC 1 cut(s) 4337
PflMI CCANNNNNTGG 5 cut(s) 430, 610, 903, 1438, 4993
PluTI GGCGCC 2 cut(s) 273, 378
PmaCI CACGTG 1 cut(s) 5129
PmlI CACGTG 1 cut(s) 5129
Ppu21I YACGTR 3 cut(s) 4109, 4892, 5129
PpuMI RGGWCCY 3 cut(s) 2079, 2340, 5297
PscI ACATGT 1 cut(s) 2894
PshBI ATTAAT 3 cut(s) 660, 3575, 4766
PsiI TTATAA 3 cut(s) 2285, 3336, 3501
Psp124BI GAGCTC 2 cut(s) 1042, 1717
Psp5II RGGWCCY 3 cut(s) 2079, 2340, 5297
Psp6I CCWGG 7 cut(s) 1537, 1708, 2104, 2925, 4268, 5154, 5259
PspCI CACGTG 1 cut(s) 5129
PspEI GGTNACC 1 cut(s) 4118
PspFI CCCAGC 2 cut(s) 153, 2613
PspGI CCWGG 7 cut(s) 1537, 1708, 2104, 2925, 4268, 5154, 5259
PspPPI RGGWCCY 3 cut(s) 2079, 2340, 5297
PsrI GAACNNNNNNTAC 2 cut(s) 4892, 4924
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
PsyI GACNNNGTC 1 cut(s) 4337
PvuII CAGCTG 1 cut(s) 2273
RruI TCGCGA 1 cut(s) 3729
RsaI GTAC 9 cut(s) 227, 735, 902, 920, 2333, 3381, 3693, 3743, 4277
RsaNI GTAC 9 cut(s) 226, 734, 901, 919, 2332, 3380, 3692, 3742, 4276
RseI CAYNNNNRTG 8 cut(s) 671, 725, 836, 2028, 2745, 3539, 3647, 4653
SacI GAGCTC 2 cut(s) 1042, 1717
SalI GTCGAC 2 cut(s) 311, 1442
SapI GCTCTTC 3 cut(s) 2252, 3480, 3632
ScaI AGTACT 3 cut(s) 227, 902, 2333
SduI GDGCHC 6 cut(s) 195, 1042, 1717, 2620, 2941, 5134
SfoI GGCGCC 2 cut(s) 271, 376
SinI GGWCC 8 cut(s) 717, 1167, 2079, 2340, 4996, 5247, 5257, 5297
SmiMI CAYNNNNRTG 8 cut(s) 671, 725, 836, 2028, 2745, 3539, 3647, 4653
SmlI CTYRAG 4 cut(s) 1409, 1566, 2335, 3948
SmoI CTYRAG 4 cut(s) 1409, 1566, 2335, 3948
SnaBI TACGTA 2 cut(s) 4109, 4892
SpeI ACTAGT 1 cut(s) 2183
SphI GCATGC 1 cut(s) 2027
SseBI AGGCCT 4 cut(s) 593, 4063, 4615, 4954
SspDI GGCGCC 2 cut(s) 269, 374
SspI AATATT 3 cut(s) 2458, 3688, 4176
SspMI CTAG 9 cut(s) 53, 2184, 3366, 4059, 4373, 4406, 4571, 4584, 5096
SstI GAGCTC 2 cut(s) 1042, 1717
StuI AGGCCT 4 cut(s) 593, 4063, 4615, 4954
StyI CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
TaiI ACGT 7 cut(s) 488, 1556, 3944, 4111, 4674, 4894, 5131
TatI WGTACW 6 cut(s) 225, 900, 918, 2331, 3379, 3691
TauI GCSGC 7 cut(s) 152, 275, 278, 354, 2723, 3192, 5036
TseFI GTSAC 8 cut(s) 481, 2755, 2836, 3121, 3397, 3942, 4118, 5273
Tsp45I GTSAC 8 cut(s) 481, 2755, 2836, 3121, 3397, 3942, 4118, 5273
TspGWI ACGGA 8 cut(s) 225, 400, 2206, 2456, 3217, 3729, 4563, 5125
Tth111I GACNNNGTC 1 cut(s) 4337
Van91I CCANNNNNTGG 5 cut(s) 430, 610, 903, 1438, 4993
VpaK11BI GGWCC 8 cut(s) 717, 1167, 2079, 2340, 4996, 5247, 5257, 5297
VspI ATTAAT 3 cut(s) 660, 3575, 4766
XagI CCTNNNNNAGG 3 cut(s) 1875, 2930, 5003
XapI RAATTY 2 cut(s) 3106, 3470
XceI RCATGY 6 cut(s) 2027, 2898, 3380, 3646, 4287, 5281
XcmI CCANNNNNNNNNTGG 4 cut(s) 1078, 4406, 4655, 5167
XmaJI CCTAGG 1 cut(s) 4058
XmiI GTMKAC 2 cut(s) 312, 1443
XmnI GAANNNNTTC 2 cut(s) 2694, 2916
XspI CTAG 9 cut(s) 53, 2184, 3366, 4059, 4373, 4406, 4571, 4584, 5096
ZraI GACGTC 1 cut(s) 486
ZrmI AGTACT 3 cut(s) 227, 902, 2333
Zsp2I ATGCAT 3 cut(s) 1954, 2025, 3922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.