Rorug01G0380500

conserved protein UCP012943

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002211
Physical Location & Seq
Reverse (-)
49308963 .. 49309744
782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0380500.1

Sequence Viewer

Length: 531 bp
ATGCCCTCGGTCGGAACCGCCATCCACATGACGGCCCTGGACGGCATCGTCACCGTGAACTCCCTCTTCACCCTCGCCGTCTTCATCGGCCTTACATGGGACCCCAATGACCCCACCAACACCCTCATCGAGCCAGAAGACTCCGCCGACTGCAGCCCCGGCCCCTCCGTCGCCGAGGACCTCGTGGCCTTCCACGTGTACTCCTTCTCCTCCTTCCTCTTCTCCAGCCTCGTCGCCCTCGCCCTCAAGCAGGGGATCCGCCTCACCCGGACCCCCTCCTACCACCCCGCCGAGATTCTCGCCCGCGTCAACAGCACCCTCCTCCGCGCCGGGATGCTCGTCTCCGGTGTCGGCTCCGTCTCCGGCTGCGTCTTCCTCATGCTCGCCTTGGTCAACGTCGTCCAGATCAAGCTCGGCACTCCCGCCTGCGGGAGCTACCACACTCTCGCCGCCATCGTTCCTCTCGTCATCTTCGTCCCCGTGGGCCTCGTCATCTATGTCGTCACTGTTGTCTATGCTTTCACTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

18.6

Weight (kDa)

5.67

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AccII CGCG 2 cut(s) 306, 327
AciI CCGC 9 cut(s) 18, 144, 259, 288, 304, 325, 423, 429, 450
AclWI GGATC 2 cut(s) 250, 263
AcvI CACGTG 1 cut(s) 196
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 6 cut(s) 11, 31, 97, 250, 428, 429
AflIII ACRYGT 1 cut(s) 195
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 412, 435
AluI AGCT 2 cut(s) 412, 435
Alw26I GTCTC 2 cut(s) 346, 364
AlwI GGATC 2 cut(s) 250, 263
AoxI GGCC 5 cut(s) 33, 88, 160, 186, 484
ApeKI GCWGC 2 cut(s) 153, 366
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 6 cut(s) 34, 100, 161, 178, 270, 484
AsuC2I CCSGG 3 cut(s) 159, 268, 331
AsuHPI GGTGA 3 cut(s) 43, 61, 256
AvaII GGWCC 3 cut(s) 100, 178, 270
BamHI GGATCC 1 cut(s) 255
BauI CACGAG 1 cut(s) 182
BbrPI CACGTG 1 cut(s) 196
BbsI GAAGAC 3 cut(s) 73, 144, 364
BbvI GCAGC 2 cut(s) 165, 353
BccI CCATC 2 cut(s) 29, 461
BceAI ACGGC 3 cut(s) 48, 58, 62
BciT130I CCWGG 1 cut(s) 38
BcnI CCSGG 3 cut(s) 159, 268, 331
BcoDI GTCTC 2 cut(s) 346, 364
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 3 cut(s) 154, 367, 450
BlsI GCNGC 3 cut(s) 155, 368, 451
Bme1390I CCNGG 4 cut(s) 38, 159, 268, 331
Bme18I GGWCC 3 cut(s) 100, 178, 270
BmgT120I GGNCC 6 cut(s) 34, 100, 161, 178, 270, 484
BmiI GGNNCC 7 cut(s) 16, 101, 102, 163, 257, 272, 355
BmrFI CCNGG 4 cut(s) 38, 159, 268, 331
BmsI GCATC 2 cut(s) 54, 324
BpiI GAAGAC 3 cut(s) 73, 144, 364
BpmI CTGGAG 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 3 cut(s) 159, 268, 331
BsaAI YACGTR 1 cut(s) 196
BsaJI CCNNGG 6 cut(s) 6, 36, 157, 174, 387, 480
BsaWI WCCGGW 1 cut(s) 344
BsaXI ACNNNNNCTCC 4 cut(s) 185, 191, 215, 221
Bsc4I CCNNNNNNNGG 6 cut(s) 11, 31, 97, 250, 428, 429
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 6 cut(s) 6, 36, 157, 174, 387, 480
BseGI GGATG 2 cut(s) 21, 339
BseLI CCNNNNNNNGG 6 cut(s) 11, 31, 97, 250, 428, 429
BseRI GAGGAG 2 cut(s) 199, 311
BseXI GCAGC 2 cut(s) 165, 353
Bsh1236I CGCG 2 cut(s) 306, 327
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 5 cut(s) 35, 90, 162, 188, 486
BsiEI CGRYCG 1 cut(s) 12
BsiSI CCGG 5 cut(s) 159, 268, 330, 345, 363
BslFI GGGAC 2 cut(s) 113, 461
BslI CCNNNNNNNGG 6 cut(s) 11, 31, 97, 250, 428, 429
BsmAI GTCTC 2 cut(s) 346, 364
BsmBI CGTCTC 2 cut(s) 346, 364
BsmFI GGGAC 2 cut(s) 113, 461
BsnI GGCC 5 cut(s) 35, 90, 162, 188, 486
Bsp143I GATC 2 cut(s) 255, 405
BspACI CCGC 9 cut(s) 18, 144, 259, 288, 304, 325, 423, 429, 450
BspANI GGCC 5 cut(s) 35, 90, 162, 188, 486
BspFNI CGCG 2 cut(s) 306, 327
BspLI GGNNCC 7 cut(s) 16, 101, 102, 163, 257, 272, 355
BspMAI CTGCAG 1 cut(s) 155
BspPI GGATC 2 cut(s) 250, 263
BssECI CCNNGG 6 cut(s) 6, 36, 157, 174, 387, 480
BssMI GATC 2 cut(s) 255, 405
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 55, 508
Bst6I CTCTTC 2 cut(s) 71, 224
BstBAI YACGTR 1 cut(s) 196
BstC8I GCNNGC 3 cut(s) 304, 384, 427
BstDSI CCRYGG 1 cut(s) 480
BstENI CCTNNNNNAGG 1 cut(s) 248
BstF5I GGATG 2 cut(s) 21, 339
BstFNI CGCG 2 cut(s) 306, 327
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 2 cut(s) 258, 408
BstMAI GTCTC 2 cut(s) 346, 364
BstMBI GATC 2 cut(s) 255, 405
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 159, 312
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 4 cut(s) 36, 157, 266, 329
BstSFI CTRYAG 1 cut(s) 151
BstUI CGCG 2 cut(s) 306, 327
BstV1I GCAGC 2 cut(s) 165, 353
BstV2I GAAGAC 3 cut(s) 73, 144, 364
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BsuRI GGCC 5 cut(s) 35, 90, 162, 188, 486
BtgI CCRYGG 1 cut(s) 480
BtsCI GGATG 2 cut(s) 21, 339
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 3 cut(s) 304, 384, 427
CfoI GCGC 1 cut(s) 329
Cfr13I GGNCC 6 cut(s) 34, 100, 161, 178, 270, 484
CseI GACGC 2 cut(s) 295, 358
Csp6I GTAC 1 cut(s) 199
CviAII CATG 3 cut(s) 28, 96, 379
CviQI GTAC 1 cut(s) 199
DpnI GATC 2 cut(s) 257, 407
DpnII GATC 2 cut(s) 255, 405
DrdI GACNNNNNNGTC 1 cut(s) 47
DseDI GACNNNNNNGTC 1 cut(s) 47
Eam1104I CTCTTC 2 cut(s) 71, 224
EarI CTCTTC 2 cut(s) 71, 224
EciI GGCGGA 2 cut(s) 133, 248
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 3 cut(s) 100, 178, 270
Eco72I CACGTG 1 cut(s) 196
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoO109I RGGNCCY 2 cut(s) 100, 178
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
Esp3I CGTCTC 2 cut(s) 346, 364
FaeI CATG 3 cut(s) 31, 99, 382
FaiI YATR 5 cut(s) 29, 97, 380, 498, 516
FaqI GGGAC 2 cut(s) 113, 461
FatI CATG 3 cut(s) 27, 95, 378
FauI CCCGC 4 cut(s) 295, 311, 422, 430
Fnu4HI GCNGC 3 cut(s) 154, 367, 450
FokI GGATG 2 cut(s) 8, 346
Fsp4HI GCNGC 3 cut(s) 154, 367, 450
GlaI GCGC 1 cut(s) 328
GluI GCNGC 3 cut(s) 154, 367, 450
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 5 cut(s) 35, 90, 162, 188, 486
HapII CCGG 5 cut(s) 159, 268, 330, 345, 363
HgaI GACGC 2 cut(s) 295, 358
HhaI GCGC 1 cut(s) 329
Hin1II CATG 3 cut(s) 31, 99, 382
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 2 cut(s) 310, 394
HindII GTYRAC 2 cut(s) 310, 394
HinfI GANTC 2 cut(s) 140, 295
HpaII CCGG 5 cut(s) 159, 268, 330, 345, 363
HphI GGTGA 3 cut(s) 43, 61, 256
Hpy166II GTNNAC 4 cut(s) 58, 199, 310, 394
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 4 cut(s) 58, 199, 310, 394
Hpy99I CGWCG 3 cut(s) 173, 236, 401
HpyAV CCTTC 3 cut(s) 199, 214, 223
HpyCH4III ACNGT 2 cut(s) 55, 508
HpyCH4IV ACGT 2 cut(s) 195, 396
HpyCH4V TGCA 1 cut(s) 153
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 312
HpySE526I ACGT 2 cut(s) 195, 396
Hsp92II CATG 3 cut(s) 31, 99, 382
HspAI GCGC 1 cut(s) 327
KflI GGGWCCC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 255, 405
LmnI GCTCC 2 cut(s) 359, 432
Lsp1109I GCAGC 2 cut(s) 165, 353
LweI GCATC 2 cut(s) 54, 324
MaeII ACGT 2 cut(s) 195, 396
MaeIII GTNAC 2 cut(s) 49, 502
MalI GATC 2 cut(s) 257, 407
MboI GATC 2 cut(s) 255, 405
MboII GAAGA 6 cut(s) 58, 73, 149, 211, 364, 463
MflI RGATCY 1 cut(s) 255
MlyI GAGTC 1 cut(s) 134
MslI CAYNNNNRTG 1 cut(s) 26
MspI CCGG 5 cut(s) 159, 268, 330, 345, 363
MspR9I CCNGG 4 cut(s) 38, 159, 268, 331
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 2 cut(s) 306, 327
MwoI GCNNNNNNNGC 2 cut(s) 159, 312
NciI CCSGG 3 cut(s) 159, 268, 331
NdeII GATC 2 cut(s) 255, 405
NlaIII CATG 3 cut(s) 31, 99, 382
NlaIV GGNNCC 7 cut(s) 16, 101, 102, 163, 257, 272, 355
NmeAIII GCCGAG 3 cut(s) 199, 316, 393
NmuCI GTSAC 2 cut(s) 49, 502
PcsI WCGNNNNNNNCGW 3 cut(s) 453, 462, 471
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 3 cut(s) 155, 368, 451
PleI GAGTC 1 cut(s) 134
PmaCI CACGTG 1 cut(s) 196
PmlI CACGTG 1 cut(s) 196
PpsI GAGTC 1 cut(s) 134
Ppu21I YACGTR 1 cut(s) 196
PpuMI RGGWCCY 2 cut(s) 100, 178
Psp5II RGGWCCY 2 cut(s) 100, 178
Psp6I CCWGG 1 cut(s) 36
PspCI CACGTG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 7 cut(s) 16, 101, 102, 163, 257, 272, 355
PspPI GGNCC 6 cut(s) 34, 100, 161, 178, 270, 484
PspPPI RGGWCCY 2 cut(s) 100, 178
PstI CTGCAG 1 cut(s) 155
PsuI RGATCY 1 cut(s) 255
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 26
SatI GCNGC 3 cut(s) 154, 367, 450
Sau3AI GATC 2 cut(s) 255, 405
Sau96I GGNCC 6 cut(s) 34, 100, 161, 178, 270, 484
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 4 cut(s) 38, 159, 268, 331
SetI ASST 5 cut(s) 183, 198, 399, 414, 437
SfaNI GCATC 2 cut(s) 54, 324
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 3 cut(s) 100, 178, 270
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SsiI CCGC 9 cut(s) 18, 144, 259, 288, 304, 325, 423, 429, 450
StyD4I CCNGG 4 cut(s) 36, 157, 266, 329
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 55, 508
TaiI ACGT 2 cut(s) 198, 399
TaqI TCGA 2 cut(s) 129, 526
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 1 cut(s) 295
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 2 cut(s) 49, 502
TseI GCWGC 2 cut(s) 153, 366
Tsp45I GTSAC 2 cut(s) 49, 502
TspDTI ATGAA 1 cut(s) 73
TspGWI ACGGA 2 cut(s) 157, 346
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 3 cut(s) 100, 178, 270
XagI CCTNNNNNAGG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.