Rorug02G0108700

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001461
Physical Location & Seq
Forward (+)
9375089 .. 9378073
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0108700.1

Sequence Viewer

Length: 1182 bp
ATGATGGCTTCAGGATTTGAAATCTCGCCTCAGGTGACCGGATTTGTTAATGGGTGCTTAAAGGACACTGCTGCAATGTCTGCCCCTGCCCCTACCCCTCCCCCTGCCATGGAAGATCACAATGGTGATACTTTGGTCAATTCCAATCAAGTTGCTATTGGAAAGCCTCCGCGGAACATTTCGGTTATGAGGCATTGCACTAGCTCTGCATTCTTGTGTGAATCAGATTCAGATCTTGGAAATTTTGGCTTGAAGTCGCCTTCAATTGAGACATCCGAGTTTGTTTCTGTATTCCGTTCGGGAAGCTGTTCTGAAAAAGGACCTAAACAGTATATGGAAGATGAGTATATTTGTGTAGATGATCTTGAGGAACATCTTGGTGCTTCAGTAAACTTGCCTTCTCATGGGGCATTTTATGGGGTCTTTGATGGACACGGTGGTGTTGATGCAGCATCATTCATAAAAAAGAATCTCCTTAATTTCATTGTTCAAGACACTCATTTCCCAAATGGCCCTAAAAAAGCAATTAGGAGCGCTTTTGTGAAGGCTGATCATGCATTTGCAGATGCTACCTCTCTTGATAAGTCCTCTGGTACTACTGCTTTGACTGCCCTTATCTTAGGAAGGAACATGCTAATCGCTAATGCTGGTGATTGTCGAGCTGTATTGGGGAAGCGCGGAAGAGCAATTGAACTATCTAAAGATCACAAACCTAATTGCACCTCTGAAAGATTAAGAATCGAGAAGCTGGGTGGTGTCATCTATGATGGATACCTCAATGGCCAACTATCAGTGGCGCGTGCTCTTGGAGACTGGCATATCAAGGGTTCTAAAGGTTCAAACTGCCCCTTAAGCTCTGAGCCAGAGTTGGAGGAAATTGTCCTAACCGAGGAGGATGAATTCTTGATAATGGGATGTGATGGCTTGTGGGATGTGATGAGCAGCCAGTGTGCTGTCACAATGGTAAGAAAGGAGCTCATGCAGCATAATGATCCTGAGAGATGTTCGAAAGTACTTGTCAAGGAGGCGCTCCAACGAAACACATGTGACAACCTGACTGTCGTGGTGGTGTGTTTCTCCGAAGACCCTCCTCCTAAGACCGAAATTCCTAAATCCCACAAGAGGAGGAGCATTTCAGCTGAAGGGTTGGACCTTCTCAAGGGTGTCTTGAGCAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

393

Amino Acids

42.42

Weight (kDa)

5.52

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 100 - 351 2e-71 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 172, 678, 799
AciI CCGC 3 cut(s) 170, 172, 678
AclWI GGATC 1 cut(s) 986
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 3 cut(s) 241, 899, 1104
AcuI CTGAAG 2 cut(s) 369, 1161
AfaI GTAC 2 cut(s) 595, 1014
AfeI AGCGCT 1 cut(s) 535
AfiI CCNNNNNNNGG 5 cut(s) 109, 404, 889, 1122, 1159
AflII CTTAAG 1 cut(s) 850
AflIII ACRYGT 1 cut(s) 1043
AgsI TTSAA 6 cut(s) 20, 253, 264, 491, 692, 840
AleI CACNNNNGTG 2 cut(s) 123, 438
AluBI AGCT 7 cut(s) 204, 306, 662, 748, 855, 976, 1139
AluI AGCT 7 cut(s) 204, 306, 662, 748, 855, 976, 1139
Alw21I GWGCWC 2 cut(s) 805, 978
Alw26I GTCTC 2 cut(s) 263, 804
AlwI GGATC 1 cut(s) 986
Aor51HI AGCGCT 1 cut(s) 535
AoxI GGCC 2 cut(s) 511, 781
ApeKI GCWGC 5 cut(s) 71, 449, 942, 982, 1173
ApoI RAATTY 3 cut(s) 241, 899, 1104
Asp700I GAANNNNTTC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 536, 678, 799, 1030
AspS9I GGNCC 3 cut(s) 320, 512, 1150
AsuHPI GGTGA 3 cut(s) 46, 137, 662
AsuII TTCGAA 1 cut(s) 1007
AvaII GGWCC 2 cut(s) 320, 1150
AxyI CCTNAGG 1 cut(s) 30
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BalI TGGCCA 1 cut(s) 783
BanII GRGCYC 1 cut(s) 978
BbsI GAAGAC 1 cut(s) 1089
Bbv12I GWGCWC 2 cut(s) 805, 978
BbvI GCAGC 4 cut(s) 58, 461, 954, 994
BccI CCATC 3 cut(s) 422, 761, 914
BciVI GTATCC 1 cut(s) 764
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 263, 804
BfaI CTAG 1 cut(s) 201
BfoI RGCGCY 2 cut(s) 537, 1031
BfrI CTTAAG 1 cut(s) 850
BfuI GTATCC 1 cut(s) 764
BglII AGATCT 1 cut(s) 232
BisI GCNGC 5 cut(s) 72, 450, 943, 983, 1174
BlsI GCNGC 5 cut(s) 73, 451, 944, 984, 1175
BmcAI AGTACT 1 cut(s) 1014
Bme18I GGWCC 2 cut(s) 320, 1150
BmgT120I GGNCC 3 cut(s) 320, 512, 1150
BmsI GCATC 3 cut(s) 436, 461, 556
BpiI GAAGAC 1 cut(s) 1089
Bpu14I TTCGAA 1 cut(s) 1007
BpuEI CTTGAG 2 cut(s) 386, 1142
BsaBI GATNNNNATC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 108, 170, 888
BsaWI WCCGGW 1 cut(s) 38
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 404, 889, 1122, 1159
Bse1I ACTGG 2 cut(s) 818, 946
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 2 cut(s) 81, 193
Bse8I GATNNNNATC 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 108, 170, 888
BseGI GGATG 4 cut(s) 272, 901, 920, 937
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 5 cut(s) 109, 404, 889, 1122, 1159
BseMI GCAATG 2 cut(s) 81, 193
BseMII CTCAG 3 cut(s) 44, 849, 987
BseNI ACTGG 2 cut(s) 818, 946
BseRI GAGGAG 4 cut(s) 905, 1080, 1138, 1141
BseXI GCAGC 4 cut(s) 58, 461, 954, 994
BseYI CCCAGC 1 cut(s) 748
Bsh1236I CGCG 3 cut(s) 172, 678, 799
BshFI GGCC 2 cut(s) 513, 783
BsiHKAI GWGCWC 2 cut(s) 805, 978
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 5 cut(s) 109, 404, 889, 1122, 1159
BsmAI GTCTC 2 cut(s) 263, 804
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 2 cut(s) 513, 783
Bsp119I TTCGAA 1 cut(s) 1007
Bsp1286I GDGCHC 2 cut(s) 805, 978
Bsp143I GATC 6 cut(s) 115, 232, 361, 550, 703, 991
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 3 cut(s) 170, 172, 678
BspANI GGCC 2 cut(s) 513, 783
BspCNI CTCAG 3 cut(s) 43, 850, 988
BspFNI CGCG 3 cut(s) 172, 678, 799
BspPI GGATC 1 cut(s) 986
BspQI GCTCTTC 1 cut(s) 676
BspT104I TTCGAA 1 cut(s) 1007
BspTI CTTAAG 1 cut(s) 850
BsrDI GCAATG 2 cut(s) 81, 193
BsrI ACTGG 2 cut(s) 818, 946
BssECI CCNNGG 3 cut(s) 108, 170, 888
BssMI GATC 6 cut(s) 115, 232, 361, 550, 703, 991
BssT1I CCWWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 330, 437, 1060
Bst6I CTCTTC 1 cut(s) 676
BstAFI CTTAAG 1 cut(s) 850
BstAPI GCANNNNNTGC 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 1007
BstC8I GCNNGC 1 cut(s) 801
BstDEI CTNAG 5 cut(s) 30, 619, 858, 996, 1095
BstDSI CCRYGG 2 cut(s) 108, 170
BstEII GGTNACC 1 cut(s) 34
BstENI CCTNNNNNAGG 2 cut(s) 887, 1157
BstF5I GGATG 4 cut(s) 272, 901, 920, 937
BstFNI CGCG 3 cut(s) 172, 678, 799
BstH2I RGCGCY 2 cut(s) 537, 1031
BstHHI GCGC 4 cut(s) 536, 678, 799, 1030
BstKTI GATC 6 cut(s) 118, 235, 364, 553, 706, 994
BstMAI GTCTC 2 cut(s) 263, 804
BstMBI GATC 6 cut(s) 115, 232, 361, 550, 703, 991
BstMWI GCNNNNNNNGC 5 cut(s) 80, 554, 608, 852, 982
BstNSI RCATGY 2 cut(s) 634, 1047
BstPI GGTNACC 1 cut(s) 34
BstUI CGCG 3 cut(s) 172, 678, 799
BstV1I GCAGC 4 cut(s) 58, 461, 954, 994
BstV2I GAAGAC 1 cut(s) 1089
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 30
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 2 cut(s) 513, 783
BtgI CCRYGG 2 cut(s) 108, 170
BtsCI GGATG 4 cut(s) 272, 901, 920, 937
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 3 cut(s) 66, 798, 953
Cac8I GCNNGC 1 cut(s) 801
CfoI GCGC 4 cut(s) 536, 678, 799, 1030
Cfr13I GGNCC 3 cut(s) 320, 512, 1150
Cfr42I CCGCGG 1 cut(s) 173
Csp6I GTAC 2 cut(s) 594, 1013
CviAII CATG 6 cut(s) 109, 404, 554, 631, 979, 1044
CviQI GTAC 2 cut(s) 594, 1013
DdeI CTNAG 5 cut(s) 30, 619, 858, 996, 1095
DpnI GATC 6 cut(s) 117, 234, 363, 552, 705, 993
DpnII GATC 6 cut(s) 115, 232, 361, 550, 703, 991
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 1 cut(s) 676
EarI CTCTTC 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 976
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 978
Eco47I GGWCC 2 cut(s) 320, 1150
Eco47III AGCGCT 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 2 cut(s) 369, 1161
Eco81I CCTNAGG 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 976
EcoNI CCTNNNNNAGG 2 cut(s) 887, 1157
EcoO109I RGGNCCY 1 cut(s) 320
EcoO65I GGTNACC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 899
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 978
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 6 cut(s) 112, 407, 557, 634, 982, 1047
FalI AAGNNNNNCTT 1 cut(s) 1151
FatI CATG 6 cut(s) 108, 403, 553, 630, 978, 1043
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 5 cut(s) 72, 450, 943, 983, 1174
FokI GGATG 4 cut(s) 259, 908, 927, 944
FriOI GRGCYC 1 cut(s) 978
Fsp4HI GCNGC 5 cut(s) 72, 450, 943, 983, 1174
FspBI CTAG 1 cut(s) 201
GlaI GCGC 4 cut(s) 535, 677, 798, 1029
GluI GCNGC 5 cut(s) 72, 450, 943, 983, 1174
GsaI CCCAGC 1 cut(s) 752
HaeII RGCGCY 2 cut(s) 537, 1031
HaeIII GGCC 2 cut(s) 513, 783
HapII CCGG 1 cut(s) 39
HhaI GCGC 4 cut(s) 536, 678, 799, 1030
Hin1II CATG 6 cut(s) 112, 407, 557, 634, 982, 1047
Hin6I GCGC 4 cut(s) 534, 676, 797, 1028
HinP1I GCGC 4 cut(s) 534, 676, 797, 1028
HinfI GANTC 4 cut(s) 221, 227, 469, 738
HpaII CCGG 1 cut(s) 39
HphI GGTGA 3 cut(s) 46, 137, 662
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 7 cut(s) 226, 232, 277, 313, 727, 859, 1081
Hpy188III TCNNGA 9 cut(s) 12, 300, 365, 491, 578, 742, 904, 995, 1168
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 6 cut(s) 270, 408, 538, 618, 1136, 1163
HpyCH4III ACNGT 3 cut(s) 330, 437, 1060
HpyCH4V TGCA 8 cut(s) 74, 198, 209, 449, 557, 563, 720, 982
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 554, 608, 852, 982
HpyF3I CTNAG 5 cut(s) 30, 619, 858, 996, 1095
Hsp92II CATG 6 cut(s) 112, 407, 557, 634, 982, 1047
HspAI GCGC 4 cut(s) 534, 676, 797, 1028
Ksp22I TGATCA 1 cut(s) 550
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 6 cut(s) 115, 232, 361, 550, 703, 991
LguI GCTCTTC 1 cut(s) 676
LmnI GCTCC 4 cut(s) 531, 973, 1035, 1128
Lsp1109I GCAGC 4 cut(s) 58, 461, 954, 994
LweI GCATC 3 cut(s) 436, 461, 556
MaeI CTAG 1 cut(s) 201
MaeIII GTNAC 3 cut(s) 34, 955, 1046
MalI GATC 6 cut(s) 117, 234, 363, 552, 705, 993
MboI GATC 6 cut(s) 115, 232, 361, 550, 703, 991
MboII GAAGA 4 cut(s) 125, 350, 693, 1094
MfeI CAATTG 2 cut(s) 264, 687
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 805, 978
MlsI TGGCCA 1 cut(s) 783
MluNI TGGCCA 1 cut(s) 783
MmeI TCCRAC 3 cut(s) 849, 1057, 1128
Mox20I TGGCCA 1 cut(s) 783
Mph1103I ATGCAT 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 307
MscI TGGCCA 1 cut(s) 783
MseI TTAA 5 cut(s) 48, 59, 477, 734, 851
MslI CAYNNNNRTG 4 cut(s) 123, 214, 378, 438
Msp20I TGGCCA 1 cut(s) 783
MspA1I CMGCKG 2 cut(s) 172, 1139
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 1 cut(s) 39
MunI CAATTG 2 cut(s) 264, 687
Mva1269I GAATGC 1 cut(s) 209
MvnI CGCG 3 cut(s) 172, 678, 799
MwoI GCNNNNNNNGC 5 cut(s) 80, 554, 608, 852, 982
NcoI CCATGG 1 cut(s) 108
NdeII GATC 6 cut(s) 115, 232, 361, 550, 703, 991
NlaIII CATG 6 cut(s) 112, 407, 557, 634, 982, 1047
NmuCI GTSAC 3 cut(s) 34, 955, 1046
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 2 cut(s) 634, 1047
NspV TTCGAA 1 cut(s) 1007
OliI CACNNNNGTG 2 cut(s) 123, 438
PciI ACATGT 1 cut(s) 1043
PciSI GCTCTTC 1 cut(s) 676
PctI GAATGC 1 cut(s) 209
PdmI GAANNNNTTC 1 cut(s) 307
PfeI GAWTC 4 cut(s) 221, 227, 469, 738
PkrI GCNGC 5 cut(s) 73, 451, 944, 984, 1175
PpuMI RGGWCCY 1 cut(s) 320
PscI ACATGT 1 cut(s) 1043
Psp124BI GAGCTC 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 320
PspEI GGTNACC 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 748
PspPI GGNCC 3 cut(s) 320, 512, 1150
PspPPI RGGWCCY 1 cut(s) 320
PsuI RGATCY 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 1139
RsaI GTAC 2 cut(s) 595, 1014
RsaNI GTAC 2 cut(s) 594, 1013
RseI CAYNNNNRTG 4 cut(s) 123, 214, 378, 438
SacI GAGCTC 1 cut(s) 978
SacII CCGCGG 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 676
SaqAI TTAA 5 cut(s) 48, 59, 477, 734, 851
SatI GCNGC 5 cut(s) 72, 450, 943, 983, 1174
Sau3AI GATC 6 cut(s) 115, 232, 361, 550, 703, 991
Sau96I GGNCC 3 cut(s) 320, 512, 1150
ScaI AGTACT 1 cut(s) 1014
SduI GDGCHC 2 cut(s) 805, 978
SfaNI GCATC 3 cut(s) 436, 461, 556
Sfr303I CCGCGG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 1007
SgrBI CCGCGG 1 cut(s) 173
SinI GGWCC 2 cut(s) 320, 1150
SmiMI CAYNNNNRTG 4 cut(s) 123, 214, 378, 438
SmlI CTYRAG 4 cut(s) 365, 850, 1157, 1168
SmoI CTYRAG 4 cut(s) 365, 850, 1157, 1168
SsiI CCGC 3 cut(s) 170, 172, 678
SspMI CTAG 1 cut(s) 201
SstI GAGCTC 1 cut(s) 978
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 330, 437, 1060
TaqI TCGA 3 cut(s) 658, 741, 1007
TaqII GACCGA 1 cut(s) 1115
TatI WGTACW 1 cut(s) 1012
TfiI GAWTC 4 cut(s) 221, 227, 469, 738
Tru1I TTAA 5 cut(s) 48, 59, 477, 734, 851
Tru9I TTAA 5 cut(s) 48, 59, 477, 734, 851
TscAI CASTG 3 cut(s) 73, 798, 953
TseFI GTSAC 3 cut(s) 34, 955, 1046
TseI GCWGC 5 cut(s) 71, 449, 942, 982, 1173
Tsp45I GTSAC 3 cut(s) 34, 955, 1046
TspDTI ATGAA 3 cut(s) 448, 472, 912
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 3 cut(s) 73, 798, 953
Vha464I CTTAAG 1 cut(s) 850
VpaK11BI GGWCC 2 cut(s) 320, 1150
XagI CCTNNNNNAGG 2 cut(s) 887, 1157
XapI RAATTY 3 cut(s) 241, 899, 1104
XceI RCATGY 2 cut(s) 634, 1047
XmnI GAANNNNTTC 1 cut(s) 307
XspI CTAG 1 cut(s) 201
ZrmI AGTACT 1 cut(s) 1014
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.