AT1G15140

Oxidoreductase NAD-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002199
Physical Location & Seq
Reverse (-)
5210181 .. 5212349
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15140.2

Sequence Viewer

Length: 888 bp
ATGTCCACTCTTCCTTTCGCGCCTTCTGTCACCCATGCGCACTTTAGCCACTCCCTCTCCCCCATGTTTATCCTGCGCCATCTCCCTCTTACACGCCATCTCCGTTTATCCCGAAATAACCGCGTCGCTTCCGTTGTATCCGCCGCCGCCGTTCGCCAGGACGCTTCCCTCTGGACTCCAGCTCCTCTCTCTCTAATCGAATCAGCTGCAGAATCGCTTTTCCACATCTCAATCGACATTTCAAACGCTCCGGATCTCGTAGCTTCGTACACGAGACCCGGTCAGTATCTCCAGATCCGTGTTCCTGATGTTGAGAAGCCTTCTTTTATGGCAATCGCTTCTCCTCCTTCCTTGGCGTCTTCTCGTGGTGCTTTCGAATTCTTGGTCAAGAGCATCGCTGGTTCTACCGCGGAGATTCTCTGCGGGTTGAAGAAAGGAGAGACCGTTGAGCTTAGCTCTGTGATGGGTAATGGTTTCAATATCGATCTGATTGATCCTCCTGAGGAATATCCCACCGTTTTGATTTTCGCCACTGGATCTGGAATTAGCCCCATCCGCTCACTAATCGAATCAGGATTTGGCGCTGATAGAAGATCTGACGTAAGACTCTATTACGGGGCTAGGAACCTGAATAGGATGGCTTACCAGGAAAAGTTTAAAGAGTGGGAATCAGCGGGTGTCAAAGTTGTGCCGGTATTATCACAGCCAGATGATGGGTGGAAAGGGGAAACTGGATATGTGCAGGCTGCTTTTGCAAGGGCTAAACAGCTATCAGCTCCCAAGGCCACGGGAGCGGTGCTGTGCGGACAGAAACAAATGGCTGAGGAGATAACTTCAATGCTTGTAGCTGATGGAGTCTCAAATGACAAGCTGCTCAAAAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

295

Amino Acids

31.92

Weight (kDa)

7.79

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 177 - 256 7.2e-14 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 39
AccB7I CCANNNNNTGG 1 cut(s) 713
AccBSI CCGCTC 2 cut(s) 558, 794
AccII CGCG 3 cut(s) 20, 123, 410
AccIII TCCGGA 1 cut(s) 250
AclWI GGATC 4 cut(s) 261, 289, 488, 544
AcsI RAATTY 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 356
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 713
AgsI TTSAA 4 cut(s) 243, 430, 478, 837
AjnI CCWGG 2 cut(s) 156, 645
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 9 cut(s) 182, 206, 263, 451, 456, 769, 776, 848, 871
AluI AGCT 9 cut(s) 182, 206, 263, 451, 456, 769, 776, 848, 871
Alw26I GTCTC 3 cut(s) 268, 434, 862
AlwI GGATC 4 cut(s) 261, 289, 488, 544
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 3 cut(s) 206, 746, 871
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AspLEI GCGC 4 cut(s) 22, 40, 78, 584
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 1 cut(s) 22
AsuII TTCGAA 1 cut(s) 375
AxyI CCTNAGG 1 cut(s) 501
BauI CACGAG 2 cut(s) 271, 363
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 3 cut(s) 193, 733, 858
BccI CCATC 7 cut(s) 87, 105, 457, 560, 631, 707, 845
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 2 cut(s) 158, 647
BciVI GTATCC 1 cut(s) 148
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 268, 434, 862
BfaI CTAG 1 cut(s) 621
BfmI CTRYAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 585
BfuI GTATCC 1 cut(s) 148
BglII AGATCT 1 cut(s) 593
BisI GCNGC 5 cut(s) 144, 147, 207, 747, 872
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 5 cut(s) 145, 148, 208, 748, 873
Bme1390I CCNGG 3 cut(s) 158, 279, 647
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 3 cut(s) 158, 279, 647
BmsI GCATC 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 2 cut(s) 162, 275
Bpu10I CCTNAGC 1 cut(s) 822
Bpu1102I GCTNAGC 1 cut(s) 452
Bpu14I TTCGAA 1 cut(s) 375
BpuMI CCSGG 1 cut(s) 279
Bsa29I ATCGAT 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 356
BsaI GGTCTC 2 cut(s) 268, 434
BsaJI CCNNGG 4 cut(s) 351, 408, 780, 786
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 8 cut(s) 41, 71, 84, 114, 166, 196, 429, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 713
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 2 cut(s) 538, 736
Bse21I CCTNAGG 1 cut(s) 501
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 2 cut(s) 158, 647
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 4 cut(s) 351, 408, 780, 786
BseGI GGATG 2 cut(s) 552, 642
BseLI CCNNNNNNNGG 1 cut(s) 713
BseMII CTCAG 2 cut(s) 492, 813
BseNI ACTGG 2 cut(s) 538, 736
BseRI GAGGAG 3 cut(s) 174, 333, 839
BseXI GCAGC 3 cut(s) 193, 733, 858
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 3 cut(s) 20, 123, 410
BshFI GGCC 1 cut(s) 785
BshVI ATCGAT 1 cut(s) 483
BsiSI CCGG 3 cut(s) 251, 279, 692
BslI CCNNNNNNNGG 1 cut(s) 713
BsmAI GTCTC 3 cut(s) 268, 434, 862
BsnI GGCC 1 cut(s) 785
Bso31I GGTCTC 2 cut(s) 268, 434
Bsp119I TTCGAA 1 cut(s) 375
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 6 cut(s) 253, 294, 484, 493, 536, 593
Bsp1720I GCTNAGC 1 cut(s) 452
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 2 cut(s) 493, 814
BspDI ATCGAT 1 cut(s) 483
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 3 cut(s) 20, 123, 410
BspLI GGNNCC 1 cut(s) 626
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 4 cut(s) 261, 289, 488, 544
BspT104I TTCGAA 1 cut(s) 375
BspTNI GGTCTC 2 cut(s) 268, 434
BsrBI CCGCTC 2 cut(s) 558, 794
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 2 cut(s) 538, 736
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 4 cut(s) 351, 408, 780, 786
BssMI GATC 6 cut(s) 253, 294, 484, 493, 536, 593
BssNI GRCGYC 1 cut(s) 356
BssSI CACGAG 2 cut(s) 271, 363
BssT1I CCWWGG 2 cut(s) 351, 780
Bst2BI CACGAG 2 cut(s) 271, 363
Bst2UI CCWGG 2 cut(s) 158, 647
Bst4CI ACNGT 2 cut(s) 445, 517
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 744
BstDEI CTNAG 3 cut(s) 452, 501, 822
BstDSI CCRYGG 2 cut(s) 408, 786
BstF5I GGATG 2 cut(s) 552, 642
BstFNI CGCG 3 cut(s) 20, 123, 410
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 4 cut(s) 22, 40, 78, 584
BstKTI GATC 6 cut(s) 256, 297, 487, 496, 539, 596
BstMAI GTCTC 3 cut(s) 268, 434, 862
BstMBI GATC 6 cut(s) 253, 294, 484, 493, 536, 593
BstMWI GCNNNNNNNGC 4 cut(s) 555, 752, 782, 791
BstNI CCWGG 2 cut(s) 158, 647
BstSCI CCNGG 3 cut(s) 156, 277, 645
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 3 cut(s) 20, 123, 410
BstV1I GCAGC 3 cut(s) 193, 733, 858
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 4 cut(s) 253, 294, 536, 593
BstYI RGATCY 4 cut(s) 253, 294, 536, 593
Bsu15I ATCGAT 1 cut(s) 483
Bsu36I CCTNAGG 1 cut(s) 501
BsuI GTATCC 1 cut(s) 148
BsuRI GGCC 1 cut(s) 785
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 2 cut(s) 408, 786
BtgZI GCGATG 1 cut(s) 379
BtsCI GGATG 2 cut(s) 552, 642
BtsIMutI CAGTG 1 cut(s) 531
Cac8I GCNNGC 1 cut(s) 744
CfoI GCGC 4 cut(s) 22, 40, 78, 584
Cfr10I RCCGGY 1 cut(s) 691
Cfr42I CCGCGG 1 cut(s) 411
ClaI ATCGAT 1 cut(s) 483
CseI GACGC 3 cut(s) 112, 170, 345
Csp6I GTAC 1 cut(s) 268
CviAII CATG 2 cut(s) 35, 64
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 452, 501, 822
DpnI GATC 6 cut(s) 255, 296, 486, 495, 538, 595
DpnII GATC 6 cut(s) 253, 294, 484, 493, 536, 593
DraI TTTAAA 1 cut(s) 658
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 351, 780
Eco31I GGTCTC 2 cut(s) 268, 434
Eco81I CCTNAGG 1 cut(s) 501
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 2 cut(s) 156, 645
EcoT14I CCWWGG 2 cut(s) 351, 780
ErhI CCWWGG 2 cut(s) 351, 780
FaeI CATG 2 cut(s) 38, 67
FaiI YATR 4 cut(s) 36, 65, 329, 738
FatI CATG 2 cut(s) 34, 63
FauI CCCGC 2 cut(s) 416, 667
Fnu4HI GCNGC 5 cut(s) 144, 147, 207, 747, 872
FokI GGATG 2 cut(s) 539, 649
Fsp4HI GCNGC 5 cut(s) 144, 147, 207, 747, 872
FspAI RTGCGCAY 1 cut(s) 39
FspBI CTAG 1 cut(s) 621
FspI TGCGCA 1 cut(s) 39
GlaI GCGC 4 cut(s) 21, 39, 77, 583
GluI GCNGC 5 cut(s) 144, 147, 207, 747, 872
GsuI CTGGAG 2 cut(s) 162, 275
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 1 cut(s) 785
HapII CCGG 3 cut(s) 251, 279, 692
HgaI GACGC 3 cut(s) 112, 170, 345
HhaI GCGC 4 cut(s) 22, 40, 78, 584
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 2 cut(s) 38, 67
Hin6I GCGC 4 cut(s) 20, 38, 76, 582
HinP1I GCGC 4 cut(s) 20, 38, 76, 582
HinfI GANTC 8 cut(s) 175, 200, 212, 415, 569, 606, 668, 855
HpaII CCGG 3 cut(s) 251, 279, 692
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 6, 270
Hpy188I TCNGA 2 cut(s) 489, 598
Hpy188III TCNNGA 9 cut(s) 111, 172, 251, 292, 305, 388, 500, 540, 573
Hpy8I GTNNAC 2 cut(s) 6, 270
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 33, 330, 357
HpyCH4III ACNGT 2 cut(s) 445, 517
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 3 cut(s) 209, 742, 755
HpyF10VI GCNNNNNNNGC 4 cut(s) 555, 752, 782, 791
HpyF3I CTNAG 3 cut(s) 452, 501, 822
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 2 cut(s) 38, 67
HspAI GCGC 4 cut(s) 20, 38, 76, 582
Kpn2I TCCGGA 1 cut(s) 250
KspI CCGCGG 1 cut(s) 411
Kzo9I GATC 6 cut(s) 253, 294, 484, 493, 536, 593
LmnI GCTCC 4 cut(s) 187, 253, 781, 791
Lsp1109I GCAGC 3 cut(s) 193, 733, 858
LweI GCATC 1 cut(s) 402
MaeI CTAG 1 cut(s) 621
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 1 cut(s) 28
MalI GATC 6 cut(s) 255, 296, 486, 495, 538, 595
MbiI CCGCTC 2 cut(s) 558, 794
MboI GATC 6 cut(s) 253, 294, 484, 493, 536, 593
MboII GAAGA 3 cut(s) 351, 442, 603
MflI RGATCY 4 cut(s) 253, 294, 536, 593
MluCI AATT 2 cut(s) 377, 543
MlyI GAGTC 3 cut(s) 169, 600, 864
MnlI CCTC 8 cut(s) 65, 96, 179, 195, 354, 496, 507, 817
MroI TCCGGA 1 cut(s) 250
MseI TTAA 1 cut(s) 657
MspA1I CMGCKG 3 cut(s) 206, 410, 674
MspI CCGG 3 cut(s) 251, 279, 692
MspR9I CCNGG 3 cut(s) 158, 279, 647
MvaI CCWGG 2 cut(s) 158, 647
MvnI CGCG 3 cut(s) 20, 123, 410
MwoI GCNNNNNNNGC 4 cut(s) 555, 752, 782, 791
NciI CCSGG 1 cut(s) 279
NdeII GATC 6 cut(s) 253, 294, 484, 493, 536, 593
NlaIII CATG 2 cut(s) 38, 67
NlaIV GGNNCC 1 cut(s) 626
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 39
NspV TTCGAA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 100
PfeI GAWTC 5 cut(s) 200, 212, 415, 569, 668
PflFI GACNNNGTC 1 cut(s) 279
PflMI CCANNNNNTGG 1 cut(s) 713
PkrI GCNGC 5 cut(s) 145, 148, 208, 748, 873
PleI GAGTC 3 cut(s) 169, 600, 863
PpsI GAGTC 3 cut(s) 169, 600, 863
Psp6I CCWGG 2 cut(s) 156, 645
PspGI CCWGG 2 cut(s) 156, 645
PspN4I GGNNCC 1 cut(s) 626
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 4 cut(s) 253, 294, 536, 593
PsyI GACNNNGTC 1 cut(s) 279
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacII CCGCGG 1 cut(s) 411
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 5 cut(s) 144, 147, 207, 747, 872
Sau3AI GATC 6 cut(s) 253, 294, 484, 493, 536, 593
SchI GAGTC 3 cut(s) 169, 600, 864
ScrFI CCNGG 3 cut(s) 158, 279, 647
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 207
Sfr303I CCGCGG 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 375
SgrBI CCGCGG 1 cut(s) 411
Sse9I AATT 2 cut(s) 377, 543
SspMI CTAG 1 cut(s) 621
StyD4I CCNGG 3 cut(s) 156, 277, 645
StyI CCWWGG 2 cut(s) 351, 780
TaaI ACNGT 2 cut(s) 445, 517
TaiI ACGT 1 cut(s) 603
TaqI TCGA 5 cut(s) 198, 234, 375, 483, 567
TasI AATT 2 cut(s) 377, 543
TauI GCSGC 2 cut(s) 146, 149
TfiI GAWTC 5 cut(s) 200, 212, 415, 569, 668
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TscAI CASTG 1 cut(s) 538
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 206, 746, 871
Tsp45I GTSAC 1 cut(s) 28
TspGWI ACGGA 3 cut(s) 92, 121, 287
TspRI CASTG 1 cut(s) 538
Tth111I GACNNNGTC 1 cut(s) 279
Van91I CCANNNNNTGG 1 cut(s) 713
XapI RAATTY 1 cut(s) 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 714
XspI CTAG 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.