Rw4G028330

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002199
Physical Location & Seq
Reverse (-)
55055404 .. 55057822
2419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028330.1

Sequence Viewer

Length: 780 bp
ATGTCATTTACAATCTGCCAACCCCATGCGCACCTTAGCCCACCCCTCCCTCCCATGTCTATCCTACGCCGCCTCAACCCCTCCCACCTAACCCTCCACCGCCACCGCCGCCGCCTCGCCAGCGTCGCAGCCGCCGTACGCCAGGACACCACTCTCTGGACCCCGGCCCCGCTCTCCCGGTACCTCCAGCTCCGGGTCCCGGACGTTGAAAAGCCCTCGTTCTTGGCCATCGCGTCGCCGCCGGCCCTGGCGGCGAAGGGCGTGTTCGAGTTCTTGGTGAAGAGCGTGGAGGGGTCCATCGCTGAGCTGCTGTGCGGGTTGAAGAAGGGGGATGTTGTGGAGCTGACCCAGGCCATGGGGAAAGGCTTCGAGATTGATCAGATCGAGCCGCCGGAGAATTATCCTACGGTTCTAATTTTCGCCACGGGATCTGGAATCAGTCCAATCAGGTCTCTGATTGAATCAGGGTTTAACGCCGATAAGCGATCCGACGTGAAGCTCTTCTATGGGGCCAGAAACCTTGACAGAATGGCTTATCAGGATAGATTTAAAGATTGGGAAGCTTCAGGTGTAAAGATAGTTCCTGTATTGTCACAACCACACGACAGCTGGTCTGGACAAAGTGGTTATGTGCAGGCTGCTTTTACTAGAGCCAAGCAAGTTTATAACCCTCTCTCCACCGCTGCTGTTCTCTGTGGGCAGAAACAGATGGCTGAGGAAGTTACCTCAATTCTTTTAGCAGATGGAGTTGCAAGTGAAAAAATACTGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

259

Amino Acids

28.45

Weight (kDa)

9.11

Isoelectric Point (pI)

54.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 141 - 241 3.6e-16 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
Acc16I TGCGCA 1 cut(s) 30
Acc65I GGTACC 1 cut(s) 180
AccB1I GGYRCC 1 cut(s) 180
AccB7I CCANNNNNTGG 2 cut(s) 156, 355
AccBSI CCGCTC 1 cut(s) 172
AccII CGCG 1 cut(s) 233
AclWI GGATC 2 cut(s) 436, 480
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 1 cut(s) 772
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 2 cut(s) 138, 182
AfiI CCNNNNNNNGG 4 cut(s) 156, 193, 199, 355
AgsI TTSAA 3 cut(s) 209, 322, 461
AhdI GACNNNNNGTC 1 cut(s) 610
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 3 cut(s) 141, 246, 348
AluBI AGCT 6 cut(s) 190, 307, 343, 499, 563, 609
AluI AGCT 6 cut(s) 190, 307, 343, 499, 563, 609
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 2 cut(s) 436, 480
AoxI GGCC 5 cut(s) 165, 225, 243, 351, 510
ApeKI GCWGC 4 cut(s) 128, 307, 638, 683
ApoI RAATTY 1 cut(s) 772
Asp700I GAANNNNTTC 3 cut(s) 365, 500, 773
Asp718I GGTACC 1 cut(s) 180
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 6 cut(s) 159, 166, 196, 244, 294, 510
AsuC2I CCSGG 4 cut(s) 164, 178, 194, 200
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 3 cut(s) 159, 196, 294
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 1 cut(s) 180
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 4 cut(s) 140, 294, 625, 670
BccI CCATC 4 cut(s) 236, 305, 703, 737
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 3 cut(s) 143, 248, 350
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 4 cut(s) 164, 178, 194, 200
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 251
BlpI GCTNAGC 1 cut(s) 303
Bme1390I CCNGG 7 cut(s) 143, 164, 178, 194, 200, 248, 350
Bme18I GGWCC 3 cut(s) 159, 196, 294
BmeRI GACNNNNNGTC 1 cut(s) 610
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 6 cut(s) 159, 166, 196, 244, 294, 510
BmiI GGNNCC 7 cut(s) 161, 168, 182, 197, 198, 295, 511
BmrFI CCNGG 7 cut(s) 143, 164, 178, 194, 200, 248, 350
BpmI CTGGAG 1 cut(s) 170
Bpu10I CCTNAGC 2 cut(s) 35, 714
Bpu1102I GCTNAGC 1 cut(s) 303
BpuMI CCSGG 4 cut(s) 164, 178, 194, 200
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 5 cut(s) 162, 246, 348, 354, 423
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 193, 199, 355
Bse118I RCCGGY 1 cut(s) 241
BseBI CCWGG 3 cut(s) 143, 248, 350
BseDI CCNNGG 5 cut(s) 162, 246, 348, 354, 423
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 4 cut(s) 156, 193, 199, 355
BseMII CTCAG 2 cut(s) 294, 705
BseXI GCAGC 4 cut(s) 140, 294, 625, 670
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 5 cut(s) 167, 227, 245, 353, 512
BshNI GGYRCC 1 cut(s) 180
BsiSI CCGG 6 cut(s) 164, 178, 193, 200, 242, 392
BsiWI CGTACG 1 cut(s) 136
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 156, 193, 199, 355
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 5 cut(s) 167, 227, 245, 353, 512
Bso31I GGTCTC 1 cut(s) 456
Bsp143I GATC 4 cut(s) 376, 381, 428, 485
Bsp1720I GCTNAGC 1 cut(s) 303
Bsp19I CCATGG 1 cut(s) 354
BspANI GGCC 5 cut(s) 167, 227, 245, 353, 512
BspCNI CTCAG 2 cut(s) 295, 706
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 7 cut(s) 161, 168, 182, 197, 198, 295, 511
BspPI GGATC 2 cut(s) 436, 480
BspQI GCTCTTC 2 cut(s) 275, 506
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 5 cut(s) 162, 246, 348, 354, 423
BssMI GATC 4 cut(s) 376, 381, 428, 485
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 3 cut(s) 143, 248, 350
Bst4CI ACNGT 1 cut(s) 409
Bst6I CTCTTC 2 cut(s) 275, 506
BstC8I GCNNGC 3 cut(s) 121, 243, 636
BstDEI CTNAG 3 cut(s) 35, 303, 714
BstDSI CCRYGG 2 cut(s) 354, 423
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 233
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 4 cut(s) 379, 384, 431, 488
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 4 cut(s) 376, 381, 428, 485
BstMWI GCNNNNNNNGC 4 cut(s) 108, 120, 125, 251
BstNI CCWGG 3 cut(s) 143, 248, 350
BstSCI CCNGG 7 cut(s) 141, 162, 176, 192, 198, 246, 348
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 4 cut(s) 140, 294, 625, 670
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 5 cut(s) 167, 227, 245, 353, 512
BtgI CCRYGG 2 cut(s) 354, 423
BtgZI GCGATG 2 cut(s) 214, 283
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 1 cut(s) 337
Cac8I GCNNGC 3 cut(s) 121, 243, 636
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 6 cut(s) 159, 166, 196, 244, 294, 510
CseI GACGC 2 cut(s) 112, 222
Csp6I GTAC 2 cut(s) 137, 181
CviAII CATG 3 cut(s) 26, 55, 355
CviQI GTAC 2 cut(s) 137, 181
DdeI CTNAG 3 cut(s) 35, 303, 714
DpnI GATC 4 cut(s) 378, 383, 430, 487
DpnII GATC 4 cut(s) 376, 381, 428, 485
DraI TTTAAA 1 cut(s) 550
DriI GACNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 275, 506
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 2 cut(s) 275, 506
Eco130I CCWWGG 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 456
Eco47I GGWCC 3 cut(s) 159, 196, 294
Eco57I CTGAAG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 3 cut(s) 141, 246, 348
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 3 cut(s) 29, 58, 358
FaiI YATR 6 cut(s) 27, 56, 356, 507, 630, 666
FaqI GGGAC 1 cut(s) 182
FatI CATG 3 cut(s) 25, 54, 354
FauI CCCGC 2 cut(s) 177, 308
FbaI TGATCA 1 cut(s) 376
FokI GGATG 1 cut(s) 344
FspAI RTGCGCAY 1 cut(s) 30
FspBI CTAG 1 cut(s) 648
FspI TGCGCA 1 cut(s) 30
GlaI GCGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 5 cut(s) 167, 227, 245, 353, 512
HapII CCGG 6 cut(s) 164, 178, 193, 200, 242, 392
HgaI GACGC 2 cut(s) 112, 222
HhaI GCGC 1 cut(s) 31
Hin1II CATG 3 cut(s) 29, 58, 358
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 2 cut(s) 435, 461
HpaII CCGG 6 cut(s) 164, 178, 193, 200, 242, 392
HphI GGTGA 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 381, 456, 490
Hpy188III TCNNGA 5 cut(s) 157, 370, 432, 539, 615
Hpy99I CGWCG 3 cut(s) 128, 238, 494
HpyAV CCTTC 2 cut(s) 250, 319
HpyCH4III ACNGT 1 cut(s) 409
HpyCH4IV ACGT 2 cut(s) 204, 492
HpyCH4V TGCA 2 cut(s) 634, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 120, 125, 251
HpyF3I CTNAG 3 cut(s) 35, 303, 714
HpySE526I ACGT 2 cut(s) 204, 492
Hsp92II CATG 3 cut(s) 29, 58, 358
HspAI GCGC 1 cut(s) 29
KflI GGGWCCC 1 cut(s) 196
KpnI GGTACC 1 cut(s) 184
KroI GCCGGC 1 cut(s) 241
KroNI GCCGGC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 376, 381, 428, 485
LguI GCTCTTC 2 cut(s) 275, 506
LmnI GCTCC 2 cut(s) 195, 340
Lsp1109I GCAGC 4 cut(s) 140, 294, 625, 670
MaeI CTAG 1 cut(s) 648
MaeII ACGT 2 cut(s) 204, 492
MaeIII GTNAC 2 cut(s) 591, 721
MalI GATC 4 cut(s) 378, 383, 430, 487
MbiI CCGCTC 1 cut(s) 172
MboI GATC 4 cut(s) 376, 381, 428, 485
MboII GAAGA 3 cut(s) 292, 334, 493
MflI RGATCY 1 cut(s) 428
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 4 cut(s) 397, 414, 729, 772
MluNI TGGCCA 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 513
Mox20I TGGCCA 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 241
MroXI GAANNNNTTC 3 cut(s) 365, 500, 773
MscI TGGCCA 1 cut(s) 227
MseI TTAA 2 cut(s) 471, 549
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 2 cut(s) 609, 683
MspI CCGG 6 cut(s) 164, 178, 193, 200, 242, 392
MspR9I CCNGG 7 cut(s) 143, 164, 178, 194, 200, 248, 350
MvaI CCWGG 3 cut(s) 143, 248, 350
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 108, 120, 125, 251
NaeI GCCGGC 1 cut(s) 243
NciI CCSGG 4 cut(s) 164, 178, 194, 200
NcoI CCATGG 1 cut(s) 354
NdeII GATC 4 cut(s) 376, 381, 428, 485
NgoMIV GCCGGC 1 cut(s) 241
NlaIII CATG 3 cut(s) 29, 58, 358
NlaIV GGNNCC 7 cut(s) 161, 168, 182, 197, 198, 295, 511
NmuCI GTSAC 1 cut(s) 591
NsbI TGCGCA 1 cut(s) 30
PciSI GCTCTTC 2 cut(s) 275, 506
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdiI GCCGGC 1 cut(s) 243
PdmI GAANNNNTTC 3 cut(s) 365, 500, 773
PfeI GAWTC 2 cut(s) 435, 461
Pfl23II CGTACG 1 cut(s) 136
PflMI CCANNNNNTGG 2 cut(s) 156, 355
PfoI TCCNGGA 1 cut(s) 198
PpuMI RGGWCCY 1 cut(s) 196
PsiI TTATAA 1 cut(s) 666
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 3 cut(s) 141, 246, 348
PspGI CCWGG 3 cut(s) 141, 246, 348
PspLI CGTACG 1 cut(s) 136
PspN4I GGNNCC 7 cut(s) 161, 168, 182, 197, 198, 295, 511
PspPI GGNCC 6 cut(s) 159, 166, 196, 244, 294, 510
PspPPI RGGWCCY 1 cut(s) 196
PsrI GAACNNNNNNTAC 2 cut(s) 564, 596
PsuI RGATCY 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 609
RsaI GTAC 2 cut(s) 138, 182
RsaNI GTAC 2 cut(s) 137, 181
SapI GCTCTTC 2 cut(s) 275, 506
SaqAI TTAA 2 cut(s) 471, 549
Sau3AI GATC 4 cut(s) 376, 381, 428, 485
Sau96I GGNCC 6 cut(s) 159, 166, 196, 244, 294, 510
ScrFI CCNGG 7 cut(s) 143, 164, 178, 194, 200, 248, 350
SinI GGWCC 3 cut(s) 159, 196, 294
Sse9I AATT 4 cut(s) 397, 414, 729, 772
SspMI CTAG 1 cut(s) 648
StyD4I CCNGG 7 cut(s) 141, 162, 176, 192, 198, 246, 348
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 409
TaiI ACGT 2 cut(s) 207, 495
TaqI TCGA 3 cut(s) 267, 369, 384
TasI AATT 4 cut(s) 397, 414, 729, 772
TauI GCSGC 7 cut(s) 72, 111, 114, 134, 241, 254, 391
TfiI GAWTC 2 cut(s) 435, 461
Tru1I TTAA 2 cut(s) 471, 549
Tru9I TTAA 2 cut(s) 471, 549
TseFI GTSAC 1 cut(s) 591
TseI GCWGC 4 cut(s) 128, 307, 638, 683
Tsp45I GTSAC 1 cut(s) 591
Van91I CCANNNNNTGG 2 cut(s) 156, 355
VpaK11BI GGWCC 3 cut(s) 159, 196, 294
XapI RAATTY 1 cut(s) 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 606
XmnI GAANNNNTTC 3 cut(s) 365, 500, 773
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.