MD13G1031300.v1.1

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002199
Physical Location & Seq
Reverse (-)
2243247 .. 2246055
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1031300.v1.1.491

Sequence Viewer

Length: 888 bp
ATGTCACTTGCAATCTTCCAACCCGCATCGGTGCCCCTCCGCTCCCATGCGCACCTTAGCTCCCCCCTCCCTCCCATGTCTATCCTACGCCGCCTCAACCCCCACCACCTAAAACTTCGCCGCCACCGCTTCGCCAGCGTTGCCGCAGCCGTACGCCAAGACACCGCTCTCTGGTCCCCGGCCCCTCTCTCCGAGATAGAACCGGCAGCAGAGTCGCTCTTCCACGTCCGCGTCGACCTGTCCGACGCCCCCGACCTCGCTTCGTCCCACACGCGAGCCGGCCAGTACCTCCAGCTTCGGGTCCCTGACGAATCGAAGCCCTCGTTTCTGGCCATCGCGTCGCCTCCATCGTTGGCGTCCGCAAAAGGCGTGTTTGAGTTTTTGGTGAAGAGCGTGGCAGGGTCCACGGCGGAGCTTCTGTGCAGGCTCAAGAGAGGGGACGTGGTGGAGCTCAGCCAGGTTATGGGGAAAGGCTTCGACATTGATCGGATCGAACCGCCGGAGAATTACCCTACGGTTCTCATATTCGCCACAGGATCTGGAATCAGTCCAATCCGGTCTCTGGTCGAATCGGGGTTTAGCGCCGATAAGAGATCCGCTGTGAAGCTCTTTTATGGCGCTAGAAACCTTGACAGAATGGCGTATCAGGATAGATTTAAGGACTGGGAATCTTCAGGTGTTGAGATTGTGCCGGTATTATCACAACCACATGGCGGTTGGACGGGACAGAGTGGTTATGTGCAGGCTGCTTTTTCCAGAGCCAAGCAAATTTATAATCCTCTCTCCACAGCTGCTGTGTTATGCGGGCAGAAGCAAATGACTGAGGAGGTTACCTCAATTCTTGTAGCAGACGGTGTATCAACTGAAAACATACTGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

296

Amino Acids

32.12

Weight (kDa)

8.85

Isoelectric Point (pI)

61.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 177 - 277 2.6e-15 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
Acc16I TGCGCA 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 463
AccBSI CCGCTC 2 cut(s) 42, 167
AccI GTMKAC 1 cut(s) 234
AccII CGCG 3 cut(s) 231, 274, 338
AclWI GGATC 3 cut(s) 497, 544, 588
AcoI YGGCCR 2 cut(s) 280, 330
AcsI RAATTY 2 cut(s) 768, 880
AcuI CTGAAG 1 cut(s) 657
AcyI GRCGYC 2 cut(s) 246, 356
AfaI GTAC 2 cut(s) 153, 287
AfiI CCNNNNNNNGG 5 cut(s) 171, 298, 463, 562, 713
AjiI CACGTC 2 cut(s) 226, 442
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 6 cut(s) 60, 295, 415, 451, 607, 791
AluI AGCT 6 cut(s) 60, 295, 415, 451, 607, 791
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 3 cut(s) 497, 544, 588
AlwNI CAGNNNCTG 2 cut(s) 539, 794
AoxI GGCC 3 cut(s) 180, 280, 330
ApeKI GCWGC 4 cut(s) 146, 206, 746, 791
ApoI RAATTY 2 cut(s) 768, 880
Asp700I GAANNNNTTC 2 cut(s) 473, 881
AspLEI GCGC 3 cut(s) 52, 584, 620
AspS9I GGNCC 4 cut(s) 174, 181, 301, 402
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 397
AvaII GGWCC 3 cut(s) 174, 301, 402
BaeGI GKGCMC 1 cut(s) 36
BalI TGGCCA 1 cut(s) 332
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 4 cut(s) 158, 218, 733, 778
BccI CCATC 2 cut(s) 341, 355
BceAI ACGGC 2 cut(s) 134, 423
BcgI CGANNNNNNTGC 2 cut(s) 195, 229
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 1 cut(s) 621
BfoI RGCGCY 2 cut(s) 585, 621
BisI GCNGC 7 cut(s) 91, 121, 144, 147, 207, 747, 792
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 7 cut(s) 92, 122, 145, 148, 208, 748, 793
Bme1390I CCNGG 2 cut(s) 179, 458
Bme18I GGWCC 3 cut(s) 174, 301, 402
BmgBI CACGTC 2 cut(s) 226, 442
BmgT120I GGNCC 4 cut(s) 174, 181, 301, 402
BmiI GGNNCC 6 cut(s) 33, 176, 183, 302, 303, 403
BmrFI CCNGG 2 cut(s) 179, 458
BmrI ACTGGG 1 cut(s) 673
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 673
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 56
Bpu1102I GCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 179
BsaHI GRCGYC 2 cut(s) 246, 356
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 2 cut(s) 177, 405
BsaWI WCCGGW 1 cut(s) 555
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 404, 434
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 298, 463, 562, 713
Bse118I RCCGGY 3 cut(s) 202, 278, 691
Bse1I ACTGG 2 cut(s) 283, 668
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 177, 405
BseLI CCNNNNNNNGG 5 cut(s) 171, 298, 463, 562, 713
BseMII CTCAG 2 cut(s) 466, 813
BseNI ACTGG 2 cut(s) 283, 668
BseRI GAGGAG 1 cut(s) 839
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 4 cut(s) 158, 218, 733, 778
BsgI GTGCAG 2 cut(s) 442, 761
Bsh1236I CGCG 3 cut(s) 231, 274, 338
BshFI GGCC 3 cut(s) 182, 282, 332
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 6 cut(s) 179, 203, 279, 500, 556, 692
BsiWI CGTACG 1 cut(s) 151
BslFI GGGAC 5 cut(s) 160, 250, 287, 452, 738
BslI CCNNNNNNNGG 5 cut(s) 171, 298, 463, 562, 713
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 5 cut(s) 160, 250, 287, 452, 738
BsnI GGCC 3 cut(s) 182, 282, 332
Bso31I GGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 2 cut(s) 36, 453
Bsp143I GATC 4 cut(s) 484, 489, 536, 593
Bsp1720I GCTNAGC 1 cut(s) 452
BspANI GGCC 3 cut(s) 182, 282, 332
BspCNI CTCAG 2 cut(s) 465, 814
BspFNI CGCG 3 cut(s) 231, 274, 338
BspLI GGNNCC 6 cut(s) 33, 176, 183, 302, 303, 403
BspPI GGATC 3 cut(s) 497, 544, 588
BspQI GCTCTTC 2 cut(s) 224, 383
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 564
BsrBI CCGCTC 2 cut(s) 42, 167
BsrFI RCCGGY 3 cut(s) 202, 278, 691
BsrI ACTGG 2 cut(s) 283, 668
BssAI RCCGGY 3 cut(s) 202, 278, 691
BssECI CCNNGG 2 cut(s) 177, 405
BssMI GATC 4 cut(s) 484, 489, 536, 593
BssNI GRCGYC 2 cut(s) 246, 356
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 517, 854
Bst6I CTCTTC 2 cut(s) 224, 383
BstACI GRCGYC 2 cut(s) 246, 356
BstC8I GCNNGC 6 cut(s) 136, 276, 280, 425, 744, 806
BstDEI CTNAG 3 cut(s) 56, 452, 822
BstDSI CCRYGG 1 cut(s) 405
BstEII GGTNACC 1 cut(s) 829
BstFNI CGCG 3 cut(s) 231, 274, 338
BstH2I RGCGCY 2 cut(s) 585, 621
BstHHI GCGC 3 cut(s) 52, 584, 620
BstKTI GATC 4 cut(s) 487, 492, 539, 596
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 4 cut(s) 484, 489, 536, 593
BstMWI GCNNNNNNNGC 3 cut(s) 126, 135, 140
BstNI CCWGG 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 829
BstSCI CCNGG 2 cut(s) 177, 456
BstSLI GKGCMC 1 cut(s) 36
BstUI CGCG 3 cut(s) 231, 274, 338
BstV1I GCAGC 4 cut(s) 158, 218, 733, 778
BstX2I RGATCY 2 cut(s) 536, 593
BstYI RGATCY 2 cut(s) 536, 593
BsuRI GGCC 3 cut(s) 182, 282, 332
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 319
BtrI CACGTC 2 cut(s) 226, 442
Cac8I GCNNGC 6 cut(s) 136, 276, 280, 425, 744, 806
CaiI CAGNNNCTG 2 cut(s) 539, 794
CfoI GCGC 3 cut(s) 52, 584, 620
Cfr10I RCCGGY 3 cut(s) 202, 278, 691
Cfr13I GGNCC 4 cut(s) 174, 181, 301, 402
CseI GACGC 4 cut(s) 220, 254, 327, 345
Csp6I GTAC 2 cut(s) 152, 286
CviAII CATG 3 cut(s) 47, 76, 710
CviQI GTAC 2 cut(s) 152, 286
DdeI CTNAG 3 cut(s) 56, 452, 822
DpnI GATC 4 cut(s) 486, 491, 538, 595
DpnII GATC 4 cut(s) 484, 489, 536, 593
EaeI YGGCCR 2 cut(s) 280, 330
Eam1104I CTCTTC 2 cut(s) 224, 383
EarI CTCTTC 2 cut(s) 224, 383
EciI GGCGGA 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 564
Eco47I GGWCC 3 cut(s) 174, 301, 402
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 657
Eco91I GGTNACC 1 cut(s) 829
EcoICRI GAGCTC 1 cut(s) 451
EcoO109I RGGNCCY 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 829
EcoRII CCWGG 1 cut(s) 456
EcoT38I GRGCYC 1 cut(s) 453
FaeI CATG 3 cut(s) 50, 79, 713
FaqI GGGAC 5 cut(s) 160, 250, 287, 452, 738
FatI CATG 3 cut(s) 46, 75, 709
FauI CCCGC 2 cut(s) 31, 797
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 7 cut(s) 91, 121, 144, 147, 207, 747, 792
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 7 cut(s) 91, 121, 144, 147, 207, 747, 792
FspAI RTGCGCAY 1 cut(s) 51
FspBI CTAG 1 cut(s) 621
FspI TGCGCA 1 cut(s) 51
GlaI GCGC 3 cut(s) 51, 583, 619
GluI GCNGC 7 cut(s) 91, 121, 144, 147, 207, 747, 792
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 2 cut(s) 585, 621
HaeIII GGCC 3 cut(s) 182, 282, 332
HapII CCGG 6 cut(s) 179, 203, 279, 500, 556, 692
HgaI GACGC 4 cut(s) 220, 254, 327, 345
HhaI GCGC 3 cut(s) 52, 584, 620
Hin1I GRCGYC 2 cut(s) 246, 356
Hin1II CATG 3 cut(s) 50, 79, 713
Hin6I GCGC 3 cut(s) 50, 582, 618
HinP1I GCGC 3 cut(s) 50, 582, 618
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 5 cut(s) 212, 311, 543, 569, 668
HpaII CCGG 6 cut(s) 179, 203, 279, 500, 556, 692
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 2 cut(s) 235, 405
Hpy188I TCNGA 3 cut(s) 193, 244, 489
Hpy188III TCNNGA 4 cut(s) 430, 540, 647, 756
Hpy8I GTNNAC 2 cut(s) 235, 405
Hpy99I CGWCG 3 cut(s) 236, 248, 343
HpyCH4III ACNGT 2 cut(s) 517, 854
HpyCH4IV ACGT 2 cut(s) 225, 441
HpyCH4V TGCA 3 cut(s) 11, 423, 742
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 135, 140
HpyF3I CTNAG 3 cut(s) 56, 452, 822
HpySE526I ACGT 2 cut(s) 225, 441
Hsp92I GRCGYC 2 cut(s) 246, 356
Hsp92II CATG 3 cut(s) 50, 79, 713
HspAI GCGC 3 cut(s) 50, 582, 618
KflI GGGWCCC 1 cut(s) 301
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Kzo9I GATC 4 cut(s) 484, 489, 536, 593
LguI GCTCTTC 2 cut(s) 224, 383
LmnI GCTCC 4 cut(s) 47, 65, 412, 448
Lsp1109I GCAGC 4 cut(s) 158, 218, 733, 778
LweI GCATC 1 cut(s) 35
MaeI CTAG 1 cut(s) 621
MaeII ACGT 2 cut(s) 225, 441
MaeIII GTNAC 2 cut(s) 3, 829
MalI GATC 4 cut(s) 486, 491, 538, 595
MbiI CCGCTC 2 cut(s) 42, 167
MboI GATC 4 cut(s) 484, 489, 536, 593
MboII GAAGA 4 cut(s) 7, 211, 400, 663
MflI RGATCY 2 cut(s) 536, 593
MhlI GDGCHC 2 cut(s) 36, 453
MlsI TGGCCA 1 cut(s) 332
MluCI AATT 4 cut(s) 505, 768, 837, 880
MluNI TGGCCA 1 cut(s) 332
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 3 cut(s) 43, 267, 698
Mox20I TGGCCA 1 cut(s) 332
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 2 cut(s) 473, 881
MscI TGGCCA 1 cut(s) 332
MseI TTAA 1 cut(s) 657
Msp20I TGGCCA 1 cut(s) 332
MspA1I CMGCKG 2 cut(s) 599, 791
MspI CCGG 6 cut(s) 179, 203, 279, 500, 556, 692
MspR9I CCNGG 2 cut(s) 179, 458
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 3 cut(s) 231, 274, 338
MwoI GCNNNNNNNGC 3 cut(s) 126, 135, 140
NaeI GCCGGC 1 cut(s) 280
NciI CCSGG 1 cut(s) 179
NdeII GATC 4 cut(s) 484, 489, 536, 593
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 3 cut(s) 50, 79, 713
NlaIV GGNNCC 6 cut(s) 33, 176, 183, 302, 303, 403
NmuCI GTSAC 1 cut(s) 3
NsbI TGCGCA 1 cut(s) 51
PciSI GCTCTTC 2 cut(s) 224, 383
PcsI WCGNNNNNNNCGW 4 cut(s) 231, 240, 320, 347
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 2 cut(s) 473, 881
PfeI GAWTC 4 cut(s) 311, 543, 569, 668
Pfl23II CGTACG 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 463
PkrI GCNGC 7 cut(s) 92, 122, 145, 148, 208, 748, 793
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 301
PsiI TTATAA 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 456
PspEI GGTNACC 1 cut(s) 829
PspGI CCWGG 1 cut(s) 456
PspLI CGTACG 1 cut(s) 151
PspN4I GGNNCC 6 cut(s) 33, 176, 183, 302, 303, 403
PspPI GGNCC 4 cut(s) 174, 181, 301, 402
PspPPI RGGWCCY 1 cut(s) 301
PstNI CAGNNNCTG 2 cut(s) 539, 794
PsuI RGATCY 2 cut(s) 536, 593
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 2 cut(s) 153, 287
RsaNI GTAC 2 cut(s) 152, 286
SacI GAGCTC 1 cut(s) 453
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 2 cut(s) 224, 383
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 7 cut(s) 91, 121, 144, 147, 207, 747, 792
Sau3AI GATC 4 cut(s) 484, 489, 536, 593
Sau96I GGNCC 4 cut(s) 174, 181, 301, 402
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 179, 458
SduI GDGCHC 2 cut(s) 36, 453
SfaNI GCATC 1 cut(s) 35
SinI GGWCC 3 cut(s) 174, 301, 402
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 4 cut(s) 505, 768, 837, 880
SspMI CTAG 1 cut(s) 621
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 2 cut(s) 177, 456
TaaI ACNGT 2 cut(s) 517, 854
TaiI ACGT 2 cut(s) 228, 444
TaqI TCGA 5 cut(s) 234, 314, 477, 492, 567
TasI AATT 4 cut(s) 505, 768, 837, 880
TauI GCSGC 3 cut(s) 93, 123, 146
TfiI GAWTC 4 cut(s) 311, 543, 569, 668
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 146, 206, 746, 791
Tsp45I GTSAC 1 cut(s) 3
Van91I CCANNNNNTGG 1 cut(s) 463
VpaK11BI GGWCC 3 cut(s) 174, 301, 402
XapI RAATTY 2 cut(s) 768, 880
XcmI CCANNNNNNNNNTGG 1 cut(s) 714
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 2 cut(s) 473, 881
XspI CTAG 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.